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Long non-coding RNA (lncRNA) LINC00891 knockdown is associated with poor prognosis of lung adenocarcinoma, but the underlying mechanism remains to be further explored. Here, we found that LINC00891 expression is downregulated in lung cancer tissues and cell lines compared with that in adjacent normal tissues and normal lung epithelial cells. LINC00891 overexpression impedes cell proliferation, invasion, migration and epithelial-to-mesenchymal transition (EMT) process in lung cancer cells. Mechanistic research showed that GATA2 directly binds to LINC00891 promoter and transcriptionally regulates LINC00891 expression. Meanwhile, GATA2 was identified as a target of miR-128-3p, and it is negatively regulated by miR-128-3p. Moreover, overexpression of GATA2 suppresses lung cancer cell proliferation, invasion, migration, and EMT process. Furthermore, LINC00891 restrains the RhoA pathway activity, and treatment with CCG-1423 (a specific RhoA pathway inhibitor) antagonizes the promoting effect of LINC00891 knockdown on cell malignant behaviors. Additionally, silencing of LINC00891 promotes xenograft tumor growth, which can be reversed by administration with CCG-1423. In summary, LINC00891 regulated by the miR-128-3p/GATA2 axis restrains lung cancer cell malignant progression and hinders xenograft tumor growth by suppressing the RhoA pathway.

Recent studies have highlighted the crucial role of abiotic processes, such as photodegradation and microclimatic fluctuation, in accelerating dryland litter decomposition. In grasslands, substantial amounts of dead plant material persist upright above the soil surface after senescence, experiencing distinct microclimatic conditions compared to surface litter. However, our understanding of how ultraviolet (UV) exposure and microclimatic conditions influence their decomposition is limited. To address this knowledge gap, we conducted a field experiment manipulating UV radiation for both soil surface litter and standing litter and monitored their microclimatic conditions in a semi‐arid grassland. Our findings indicate that UV exposure enhanced the decomposition of soil surface litter by alleviating the constraint of lignin on litter decomposition, while having no significant influence on standing litter. Although the mean levels of thermal‐hydric conditions were lower, more intense fluctuation of temperature and air humidity was detected in standing litter. These higher‐level microclimatic fluctuations facilitated the release of dissolved organic carbon, potentially increasing the availability of labile substrates to microbes. Meanwhile, standing litter released more photo‐sensitive phenols, leading to decreased sensitivity to UV exposure. Consequently, while UV exposure initially increased standing litter decomposition during the early stage, its influence eventually diminished. These findings underscore the critical yet differing roles of microclimatic conditions and UV exposure in the decomposition of standing and surface litter. Relying solely on knowledges derived from surface litter decomposition and microclimate conditions may not accurately capture the patterns of grassland litter degradation. Plain Language Summary: The limited precipitation in drylands is widely believed to restrict litter decomposition. However, the observed litter decomposition rates in these regions often exceed model predictions based on climatic conditions. In arid and semi‐arid steppes, the sparse vegetation cover results in intense ultraviolet (UV) radiation reaching soil surface, thereby triggering photodegradation of the recalcitrant compounds in litter. Additionally, substantial dead plant materials persist standing for months to years, and undergoes more intense warm‐cold and wet‐dry cycles. However, there have been limited studies assessing the intricate interplay between UV radiation and microclimate fluctuations on litter decomposition in drylands. In this study, we found that UV radiation facilitated the degradation of recalcitrant compounds and enhanced litter degradability of soil surface litter. More intense temperature and humidity fluctuations increased the concentration of dissolved organic carbon in standing litter. This increase could provide more labile substrate to decomposers, consequently accelerating litter decomposition. Moreover, microclimatic fluctuation enhances the loss of photo‐sensitive compounds in litter, reducing litter's sensitivity to UV exposure. Our study highlighted the significant role played by the interaction between climatic conditions and UV radiation in driving litter decomposition in water‐limited steppes, contributing to a better understanding of carbon turnover in dryland ecosystem. Key Points: The degradation of soil surface litter was mainly driven by warmer and wetter microclimate and ultraviolet (UV) exposureGreater microclimatic fluctuation in standing litter enhanced the release of dissolved labile carbon, thereby accelerating its decompositionGreater microclimatic fluctuation increased the loss of photo‐sensitive compound and weakened sensitivity of standing litter to UV radiation [ABSTRACT FROM AUTHOR]

In proton acceleration from laser-irradiated thin foil targets, adding foams on the front surface or connecting a helical coil on the rear surface of the foil has proven to be an effective scheme to enhance proton energy. In this paper, we make the first attempt to incorporate the above two enhancement schemes for laser-proton acceleration by simultaneously adding foams and connecting a helical coil to a thin foil target. By utilizing such integrated targets in the experiment, focused beams were generated. The maximum proton energy and the number of energetic protons are apparently enhanced. Moreover, quasi-monoenergetic peaks were formed at the high-energy end of the spectra. Particle-in-cell plasma simulations and electromagnetic beam dynamics simulations show that the double-layer target not only enhances the energy of protons but also leads to a multiple-fold increase in the number of escaped electrons, which results in an enhanced post-acceleration in helical coil subsequently. [ABSTRACT FROM AUTHOR]

The associations of arbuscular mycorrhizal (AM) or ectomycorrhiza (EcM) fungi with plants have sequentially evolved and significantly contributed to enhancing plant nutrition. Nonetheless, how evolutionary and ecological forces drive nutrient acquisition strategies of AM and EcM woody plants remains poorly understood. Our global analysis of woody species revealed that, over divergence time, AM woody plants evolved faster nitrogen mineralization rates without changes in nitrogen resorption. However, EcM woody plants exhibited an increase in nitrogen mineralization but a decrease in nitrogen resorption, indicating a shift towards a more inorganic nutrient economy. Despite this alteration, when evaluating present‐day woody species, AM woody plants still display faster nitrogen mineralization and lower nitrogen resorption than EcM woody plants. This inorganic nutrient economy allows AM woody plants to thrive in warm environments with a faster litter decomposition rate. Our findings indicate that the global pattern of nutrient acquisition strategies in mycorrhizal plants is shaped by the interplay between phylogeny and climate. [ABSTRACT FROM AUTHOR]

Nitrogen (N) retention is a critical ecosystem function for maintaining soil fertility and mitigating pollution caused by anthropogenic N input. However, it has not yet been elucidated how responses of plant and soil regulate ecosystem N retention. Here, we combined a 14‐year N addition experiment in a temperate steppe with a global meta‐analysis in grasslands, to assess changes in carbon (C) pool size and stoichiometric C:N ratio of plant and soil components and evaluate the contribution of each component to grassland N retention under increasing N levels. We found that N addition increased N storage in the plant pool by stimulating biomass production and reducing tissue C:N at the community level. However, the non‐random loss of forbs and legumes associated with a low C:N ratio partially offset the decline in community‐level C:N ratio, thereby diminishing the positive net effect of N enrichment on plant N storage. The observed increase in soil N storage was predominantly determined by the decrease in C:N ratio of topsoil, while no changes were detected in the subsoil. On 14‐year time scale, the upper limitation of N retention capacity in our study site was 167.02 g N/m2. Global meta‐analysis further indicated that a decade's N addition significantly increased the N storage in shoot, root and topsoil through enhancing the C pool and decreasing the C:N ratio, while did not affect those of subsoil. However, the positive correlation between the response of subsoil N storage and treatment duration further indicates that, though the accumulation of added N lagged behind that of topsoil, subsoil could play an important role in N retention on a longer time scale. Our study demonstrated that the enhanced plant productivity and altered physiological metabolism indicated by the decreased C:N ratio jointly determined grassland ecosystem N retention. The capacity of the grassland ecosystem to retain exogenous N input is limited, especially for a large amount of N input that occurs in a short period. However, in the context of chronically rising N deposition, the long‐term N retention capacity of grasslands should largely depend on the response of subsoil, especially after topsoil N is saturated. [ABSTRACT FROM AUTHOR]

Increased atmospheric nitrogen (N) deposition significantly disturbs ecosystem N cycle. Although foliar interception and uptake of N deposition can provide an important alternative N supply to forest ecosystems, the mechanisms regulating foliar N uptake from wet deposition are not fully understood. Here, we selected 19 woody species with a wide range of plant traits from different functional groups and conducted a 15N isotope labelling experiment through brushing 15NH4+ and 15NO3− solution on canopy leaves. Our findings demonstrate that leaves can directly absorb N from wet deposition within a few hours. The average leaf 15N recoveries were 10% and 28% under 15NH4+ and 15NO3− treatments across species, respectively, while twig N recoveries were only 1%–7% of leaf N recoveries. Differences in foliar N uptake efficiency among species were closely associated with leaf traits but were little influenced by meteorological conditions or soil nutrient status. Specifically, plants with higher leaf N concentration, larger specific leaf area and lower wax concentration exhibited higher leaf N recovery. Our results indicated that tree canopies could directly absorb N from atmospheric deposition. We highlight the critical role of leaf traits in determining canopy foliar N uptake, which may consequently influence plant competition under elevated N deposition. [ABSTRACT FROM AUTHOR]