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Your search keyword '"Perla R. Ardiles"' showing total 2 results
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2 results on '"Perla R. Ardiles"'

1. Preparation and Study of the Antibacterial Applications and Oxidative Stress Induction of Copper Maleamate-Functionalized Mesoporous Silica Nanoparticles

Author

Diana Díaz-García, Perla R. Ardiles, Sanjiv Prashar, Antonio Rodríguez-Diéguez, Paulina L. Páez, and Santiago Gómez-Ruiz

Subjects
mesoporous silica nanoparticles, maleamates, copper, antibacterial, ROS, oxidation reactions, Pharmacy and materia medica, RS1-441
Abstract

Mesoporous silica nanoparticles (MSNs) are an interesting class of nanomaterials with potential applications in different therapeutic areas and that have been extensively used as drug carriers in different fields of medicine. The present work is focused on the synthesis of MSNs containing a maleamato ligand (MSN-maleamic) and the subsequent coordination of copper(II) ions (MSN-maleamic-Cu) for the exploration of their potential application as antibacterial agents. The Cu-containing nanomaterials have been characterized by different techniques and the preliminary antibacterial effect of the supported maleamato-copper(II) complexes has been tested against two types of bacteria (Gram positive and Gram negative) in different assays to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biological results showed a moderate antibacterial activity against Escherichia coli which motivated a more detailed study of the antibacterial mechanism of action of the synthesized maleamate-containing nanosystems and whose findings showed oxidative stress generation in bacterial cells. All the prepared nanomaterials were also tested as catalysts in the “solvent free” selective oxidation of benzyl alcohol, to observe if there is a potential correlation between the catalytic oxidation capacity of the materials and the observed oxidative stress in bacteria. This may help in the future, for a more accurate rational design of antibacterial nanosystems, based on their observed catalytic oxidation activity.

Published
2019
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2. Copper-functionalized nanostructured silica-based systems: Study of the antimicrobial applications and ROS generation against gram positive and gram negative bacteria

Author

Paulina Laura Páez, Miguel Díaz-Sánchez, Sanjiv Prashar, Diana Díaz-García, Irene Mena-Palomo, Isabel del Hierro, Antonio Rodríguez-Diéguez, Perla R. Ardiles, and Santiago Gómez-Ruiz

Subjects
Gram-negative bacteria, Tripeptide, 010402 general chemistry, Gram-Positive Bacteria, 01 natural sciences, Biochemistry, Inorganic Chemistry, Minimum inhibitory concentration, Gram-Negative Bacteria, Organometallic Compounds, Minimum bactericidal concentration, biology, 010405 organic chemistry, Chemistry, Mesoporous silica, biology.organism_classification, Antimicrobial, Silicon Dioxide, 0104 chemical sciences, Anti-Bacterial Agents, Oxidative Stress, Nanoparticles, Antibacterial activity, Mesoporous material, Reactive Oxygen Species, Copper, Nuclear chemistry
Abstract

A series of copper-functionalized SBA-15 (Santa Barbara Amorphous) materials containing the ligands triethoxysilylpropylmaleamic acid (maleamic) or triethoxy-3-(2-imidazolin-1-yl)propylsilane (imidazoline) have been prepared. The nanostructured silica-based systems SBA-maleamic, SBA-imidazoline, SBA-maleamic-Cu and SBA-imidazoline-Cu were characterized by several methods observing that the functionalization took place mainly inside the pores of the mesoporous system. The antimicrobial behaviour of the synthesized materials against Staphylococcus aureus and Escherichia coli was tested observing a very potent activity of the copper-functionalized systems (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for SBA-maleamic-Cu of ca. 31.25 μg/mL, which correspond with ca. 1.13 μg/mL of Cu). A study of the oxidative stress promoted by the synthesized materials showed that the SBA-maleamic-Cu and the SBA-imidazoline-Cu were able to increase the reactive oxygen species (ROS) production in S. aureus by 427% and 373%, respectively, while this increase was slightly lower in E. coli (387 and 324%, respectively). Furthermore, an electrochemical study was carried out in order to determine if these materials interact with lysine or alanine to validate a potential antimicrobial mechanism based on the inhibition of the synthesis of the peptidoglycan of the bacterial wall. Finally, these studies were also performed to determine the potential interaction of the copper-containing materials with glutathione in order to assess if they are able to perturb the metabolism of this tripeptide.

Published
2019

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