Search

The kinetics of change in peroxide value and weight gain were simultaneously studied during the peroxidation of three vegetable oils of various chemical compositions. The initiation and propagation oxidizability parameters Oi (kg mEq−1 h2) and rn (h−1) of the canola, corn, and peanut oils were calculated to be 412 and 0.0894, 160 and 0.0641, and 36.1 and 0.0304, respectively. The weight-based parameter Oiw ranked the oils (103,000, 51,000, and 8000% h2, respectively) as the parameter Oi did. However, the equivalent parameter rnw (0.0680, 0.1198, and 0.1875 h−1, respectively) provided a reverse order than that of the parameter rn. This was attributed to the fact that the value of rn represents the formation and decomposition of lipid hydroperoxides in general, whereas the value of rnw actually represents the overall tendency of an oil to the formation and polymerization of lipid hydroperoxides. [ABSTRACT FROM AUTHOR]

Phospholipid (PL) peroxidation determines ferroptosis sensitivity, which is primarily regulated by the substrates, process, and detoxification of PL peroxidation. B-ring polyunsaturated sterols can directly inhibit the PL peroxidation process as radical-trapping antioxidants (RTAs), and are highly conserved in many species. Sterol metabolism regulates ferroptosis through indirect mechanisms such as regulating PL remodeling and regulating RTA content. Targeting the relevant factors involved in sterol regulation provides promising insights into the treatments of ferroptosis-associated diseases. Ferroptosis, a novel cell death mode driven by iron-dependent phospholipid (PL) peroxidation, has emerged as a promising therapeutic strategy for the treatments of cancer, cardiovascular diseases, and ischemic-reperfusion injury (IRI). PL peroxidation, the key process of ferroptosis, requires polyunsaturated fatty acid (PUFA)-containing PLs (PL-PUFAs) as substrates, undergoing a chain reaction with iron and oxygen. Cells prevent ferroptosis by maintaining a homeostatic equilibrium among substrates, processes, and detoxification of PL peroxidation. Sterols, lipids abundant in cell membranes, directly participate in PL peroxidation and influence ferroptosis sensitivity. Sterol metabolism also plays a key role in ferroptosis, and targeting sterols presents significant potential for treating numerous ferroptosis-associated disorders. This review elucidates the fundamental mechanisms of ferroptosis, emphasizing how sterols modulate this process and their therapeutic potential. [ABSTRACT FROM AUTHOR]

Lipid peroxidation plays an important role in physiological and pathophysiological conditions; it occurs mainly in the vicinity of the membrane. Glycerophospholipids are indispensable for normal structure of membranes. Lipid mediators are final products of lipid peroxidation. Glycerophospholipids as a source of lipid mediators are closely related with lipid mediators in lipid peroxidation process. This study developed a strategy for simultaneous analysis of glycerophospholipids and lipid mediators in a sample based on a secondary extraction scheme. Due to differences in physicochemical properties of glycerophospholipids and lipid mediators, we used different solvents and methods to extract glycerophospholipids and lipid mediators based on a sample. In addition, we compared three methods of extracting glycerophospholipids to select the best extraction method. In this study, a UPLC-MRM absolute quantification strategy for three deuterated internal standards of lipid mediator was established. The calibration range of all standards is linear, and the correlation coefficients are greater than 0.999. Quantitative range, precision, and accuracy of all analytes–based analysis method meet requirements of method validation. And the accuracy and precision of this method for glycerophospholipids meet the requirements of method verification. It is satisfactory to apply this method in the analysis of the changes of enzymes in lipid peroxidation during the treatment of asthma with dexamethasone. The quantitative results of phospholipids and lipid mediators in mouse lung tissue were obtained by this method, indicating that dexamethasone may inhibit the activity of phospholipase As enzyme and thus slow down the lipid peroxidation process. This work may be of great significance for exploring the changes of enzymes in lipid peroxidation metabolism. [ABSTRACT FROM AUTHOR]

Hypoxia/reoxygenation (HR) often occurs under cardiac pathological conditions, and HR-induced oxidative stress usually leads to cardiomyocyte damage. Carvedilol, a non-selective β-blocker, is used clinically to treat cardiac ischemia diseases. Moreover, Carvedilol has also been reported to have an antioxidant ability by reducing lipid peroxidation. However, the mechanism of Carvedilol to inhibit lipid peroxidation is still elusive. To explore the protective mechanism of Carvedilol to resist lipid peroxidation on cardiomyocytes, HL-1 cells were cultured under normoxia, hypoxia, and HR and treated with Carvedilol to investigate the alteration on metabolism, protein expression, and mRNA level to explain its oxidative mechanism. The study found that Carvedilol upregulated glutathione peroxidase 4 (GPX4) protein expression to resist HR-induced lipid peroxidation by metabolic remodeling under HR. Also, Carvedilol promoted ferroptosis-related genes, ferritin heavy chain 1 (FTH1) and ferritin light chain 1 (FTL1) mRNA levels, to reduce lipid peroxidation under both hypoxia and HR. In conclusion, our study explores a mechanism by which Carvedilol inhibits ferroptosis by upregulating GPX4, FTH1, and FTL1 levels to downregulate lipid peroxidation under HR. The study provides a potential strategy for using Carvedilol in clinical applications, inspiring further research and development in the area of heart diseases. [ABSTRACT FROM AUTHOR]

The human body contains ~1014 cells—each of which is separated by a lipid bilayer, along with its organeller. Unsaturated fatty acids are located on the external layer and, as a result, are particularly exposed to harmful factors, including xenobiotics and ionising radiation. During this activity, lipid peroxidation products are generated, e.g., 4-hydroxy-2-nonenal (HNA), 4-oxo-2(E)-nonenal (ONE), and malondialdehyde (MDA). The mentioned aldehydes can react with cytosolic 2′-deoxynucleosides via Michael addition. In this paper, the following adducts have been taken into theoretical consideration: ε-dCyt, H-ε-dAde, ε-dCyt, H-ε-dAde, H-ε-dGua, R/S-OH-PdGua, N2,3-ε-dGua, M1-dGua, N1-ε-dGua, and HNE-dGua. The presence of the above molecules can alter a cell's antioxidant pool. With this in mind, the adiabatic ionisation potential (AIP) and vertical ionisation potential (VIP), as well as the spin and charge distributions, are discussed. For this purpose, DFT studies were performed at the M06-2x/6-31++G** level of theory in the aqueous phase (both non-equilibrated (NE) and equilibrated (EQ) solvent–solute interaction modes), together with a Hirshfeld charge and spin distribution analysis. The obtained results indicate that the AIPs of all the investigated molecules fell within a range of 5.72 and 5.98 eV, which is consistent with the reference value of 7,8-dihydro-8-oxo-2′-deoxyguanosine (OXOdGua), 5.78 eV. N2,3-ε-dGua and M1-dGua were the only exceptions, whose VIP and AIP were noted as higher. The electronic properties analysis of 2′-deoxynucleoside adducts with lipid peroxidation products reveals their potential influence on the cells' antioxidant pool, whereby they can affect the communication process between proteins, lipids, and nucleotides. [ABSTRACT FROM AUTHOR]

Ferroptosis, an iron-dependent form of regulated cell death, has recently emerged as a crucial process in the pathogenesis of Pyricularia oryzae , the causal agent of the devastating rice blast disease, which causes billions of dollars in annual losses. This mini review explores the potential of antioxidants in suppressing ferroptosis in P. oryzae to promote sustainable rice production, with significant implications for global food security and nutrition. We critically analyze the current literature on the mechanisms of ferroptosis in P. oryzae , including iron metabolism and lipid peroxidation, the role of different antioxidants in inhibiting this cell death pathway, and the potential applications of antioxidant-based strategies for the management of rice blast disease. Recent discoveries, such as the efficacy of the natural flavonoid tangeretin in inhibiting fungal ferroptosis by interfering with the accumulation of iron and reactive oxygen species, highlight the promise of natural and nature-inspired compounds for disease management. The use of antioxidants to modulate ferroptosis in P. oryzae offers several advantages over traditional fungicide-based approaches, including improved safety, sustainability, and potential nutritional benefits through antioxidant-enriched rice varieties. However, challenges such as optimizing delivery methods, managing potential resistance, and ensuring efficacy under different environmental conditions need to be addressed. To achieve these goals, future research should focus on identifying the most effective antioxidant compounds, exploring synergistic combinations, and developing sustainable application methods. [ABSTRACT FROM AUTHOR]

High-concentration oxygen inhalation is the primary intervention to prevent perioperative hypoxemia. However, there are concerns that this may induce an imbalance in oxidation‒reduction processes, particularly in pediatric patients with compromised antioxidant defenses. This study aimed to evaluate the impact of varying intraoperative concentrations of oxygen inhalation on oxidative stress in children by analyzing plasma biomarkers, oxygenation indices, and the duration of surgery and oxygen inhalation. Forty-five children scheduled for laparoscopic pyeloplasty under general anesthesia were randomly assigned to three groups, each receiving different fractions of inspired oxygen during surgery: 30%, 50%, or 80%. The primary outcome was the plasma concentration of oxidative stress markers, and the other measurements included the surgical duration and duration of oxygen exposure. Thirty-five children completed the study, with 11 in the low group, 12 in the medium group and 12 in the high group. The levels of superoxide dismutase at immediately post-tracheal intubation, hydrogen peroxide at 1 hour post-intubation, and 8-isoprostane at immediately post-surgical procedure were significantly higher in the high group than in the low group. The S100B levels at immediately post-surgical procedure were higher than those at immediately post-tracheal intubation and 1 hour post-intubation within the low group. Therefore, we conclude that inhaling a high concentration of oxygen during laparoscopic pyeloplasty under general anesthesia, for a duration of less than 3 hours, does not significantly increase oxidative stress in pediatric patients. This study was registered at the Chinese Clinical Trial Registry (registration No. ChiCTR2400083143). [ABSTRACT FROM AUTHOR]

Organic peroxides have become sought-after functionalities, particularly following the multi-tone consumption in polymer production and success in medicinal chemistry. The selective introduction of a peroxide fragment at different positions on the target molecule is a priority in the modern reaction design. The pioneering Kharasch–Sosnovsky peroxidation became the basic universal platform for the development of peroxidation methods, with its great potential for rapid generation of complexity due to the ability to couple the resulting free radicals with a wide range of partners. This review discusses the recent advances in the radical Kharasch-type functionalization of organic molecules with OOR fragment including free-component radical couplings. The discussion has been structured by the type of the substrate of radical peroxidation: C(sp3)–H substrates; aromatic systems; compounds with unsaturated C–C or C–Het bonds.

Macrocycles bridge the gap between conventional small molecules and polymers. Drawing inspiration from successful carbon heteroatom-containing macrocycles, peroxide-containing macrocycles are gaining attention for enhanced bioactivity, potential chelating properties, and applications in energetic materials. This review presents the following strategies for the construction of cyclic peroxides with 10- to 36-membered frameworks: (1) the intramolecular iodocyclization of hydroperoxides, (2) the intermolecular cyclization of hydroperoxides with alkyl dihalides or carbonyls, (3) the acid-catalyzed rearrangements of ozonides or 11-membered cyclic triperoxides via oxy- or peroxycarbenium ions, and (4) the peroxidation of carbonyls targeting macrocyclic peroxides. The specific agents that allow for the selective construction of the medium and large cycles are also analyzed.

Phenols play a crucial role as core structural motifs in natural products and also serve as fundamental building blocks in synthetic chemistry. Apart from the known protocols for the conversion of aryl precursors to phenols (i. e., decarboxylative oxygenation), we report here the efficient synthesis of phenols from the stable and readily available benzylic carboxylic acids under mild reaction conditions. The photocatalytic conversion of carboxylic acids to peroxides is a crucial step in this strategy, allowing the subsequent C−O bond formation via Hock rearrangement. [ABSTRACT FROM AUTHOR]

Flooding causes severe yield losses worldwide, making it urgent to enhance crop tolerance to this stress. Since natural flooding often involves physical flow, we hypothesized that the effects of submergence on plants could change when combined with physical flow. In this study, we analyzed the growth and transcriptome of Arabidopsis thaliana exposed to submergence or flooding with physical flow. Plants exposed to flooding with physical flow had smaller rosette diameters, especially at faster flow rates. Transcriptome analysis revealed that "defense response" transcripts were highly up-regulated in response to flooding with physical flow. In addition, up-regulation of transcripts encoding ROS-producing enzymes, SA synthesis, JA synthesis, and ethylene signaling was more pronounced under flooding with physical flow when compared to submergence. Although H2O2 accumulation changed in response to submergence or flooding with physical flow, it did not lead to lipid peroxidation, suggesting a role for ROS as signaling molecules under these conditions. Multiple regression analysis indicated possible links between rosette diameter under flooding with physical flow and the expression of Rbohs and SA synthesis transcripts. These findings suggest that pathogen defense responses, regulated by SA and ROS signaling, play crucial roles in plant responses to flooding with physical flow. [ABSTRACT FROM AUTHOR]

New 4-hydroxycoumarins and coumestans with tert-butyl and isobornyl substituents were synthesized. The cytotoxic profile and the antioxidant status of the synthesized compounds were assessed using in vitro models. The promise of the coumestans studied as potential antitumor agents is demonstrated. A possible mechanism of the action of the title compounds is associated with the antioxidant activity expressed in the ability to inhibit the process of lipid peroxidation and with the direct antiradical activity in the ORAC and DPPH assays. [ABSTRACT FROM AUTHOR]

The experiment on mung bean (Vigna radiata) in 2023 commenced at the laboratory of the University of Kerbala, Kerbala, Iraq. It aimed to determine the role of trehalose and glutathione in reducing cadmium (Cd) toxicity. The mung bean cuttings served as an experimental material to evaluate the best method of applying trehalose and glutathione in reducing Cd toxicity. Root response, antioxidant defense, and peroxidation were the indicators used. With pre- and post-treatment of trehalose and glutathione and in combination with Cd, significant differences occurred for these treatments. By treating with Cd, trehalose and glutathione were able to remove Cd toxicity and showed an increase in rooting response. By using trehalose and glutathione together in mung bean, and using Cd during the first 24 hours, their effect was inhibitory. However, when treated together with the toxic Cd, the trehalose and glutathione removed the Cd toxicity in mung bean, improved the rooting response, and raised the levels of GSH, ASA, and CAT, and SOD activity. In addition, they decreased the level of MDA and the lipoxygenase activity compared with the control treatment. The study concluded that the combination of trehalose and glutathione showed a promising protection for mung bean plants from Cd toxicity. [ABSTRACT FROM AUTHOR]

Summary: This study aimed to investigate the effects of different oxidation modes on the functional and gel properties of hazelnut proteins. The solubility of hazelnut protein showed a small trend when the oxidation level was low, and it decreased significantly with the increase in concentration. The maximum value of water‐holding capacity (WHC), emulsifying activity index (EAI), and emulsion stability index (ESI) was 343.33%, 56.00 m2 g−1, and 75.85 min at AAPH concentration of 1.0 mmol L−1, respectively. The functional properties of hazelnut proteins, except oil‐holding capacity (OHC), gradually reduced with the increase in malondialdehyde (MDA) concentration. H2O2 modified improved OHC, foaming capacity, foam stability, and EAI of hazelnut proteins, whereas WHC and ESI gradually decreased. The results of the secondary structure of hazelnut protein gels indicated a high ratio of α‐helix and β‐fold of the gels. Also, the increase in oxidation led to an increase in the gels' voids. Also, oxidation led to a rough and loose network structure of hazelnut protein gels. These results suggested that peroxyl radical, hydroxyl radical, and MDA produced during lipid peroxidation affected the interfacial properties of hazelnut protein and disrupted the formation of hazelnut protein gel, thus affecting the quality of hazelnut protein. [ABSTRACT FROM AUTHOR]

In this study, the effects of black carrot juice on acutely exercised rat liver tissues were investigated. Rats were formed into Control (C), Black carrot juice (S), Acute exercise (E) and Black carrot juice + Acute exercise (SE) groups. The acute exercise program was applied on a treadmill device. Malondialdehyde (MDA) and Glutathione (GSH) levels were examined in the rat liver tissues obtained at the end of the experimental process, while Glutathione reductase (GR), glutathione-S-transferase (GST) and Carboxyl esterase (Ces) enzyme activity levels were measured. While MDA, GST and Ces enzyme activity levels in the E group increased compared to the K group, GR and GSH levels decreased compared to the K group. GST and Ces enzyme activities in the S group increased compared to the K group. While GSH level in the SE group increased compared to the E group, GST enzyme activity and MDA levels decreased. As a result, we think that black carrot juice prevents lipid peroxidation and has a supportive effect on the antioxidant enzyme system on the liver tissues of rats subjected to acute exercise. [ABSTRACT FROM AUTHOR]

This review highlights the progress made in recent years on biosensors aimed at detecting relevant analytes/markers of food peroxidation. Starting from the basic definition of biosensors and the chemical features of peroxidation, here we describe the different approaches that can be used to obtain information about the progress of peroxidation and the efficacy of antioxidants. Aptamers, metal–organic frameworks, nanomaterials, and supported enzymes, in conjunction with electrochemical methods, can provide fast and cost-effective detection of analytes related to peroxidation, like peroxides, aldehydes, and metals. The determination of (poly)phenols concentrations by biosensors, which can be easily obtained by using immobilized enzymes (like laccase), provides an indirect measure of peroxidation. The rationale for developing new biosensors, with a special focus on food applications, is also discussed. [ABSTRACT FROM AUTHOR]

It is well known, that antitumor drug cisplatin is an antineoplastic drug which widely used in chemotherapy. However, the usage has been limited due to cisplatin-caused various side effects. It has been established that toxic effects of cisplatin are the result of oxidative stress. Oxidative stress is the result of the excessive formation of reactive oxygen species, that can be induced by cisplatin. The reactive oxygen species in turn can interact with DNA, lipids and proteins, leading to lipid peroxidation and DNA damage. Dexamethasone is being used in chemotherapy practice as concomitant agent to mitigate the side effects of antitumor drug cisplatin. It is known that both cisplatin and dexamethasone are capable of stimulating the production of reactive oxygen species, which in turn target various biomolecules, including lipids. The aim of this study was to evaluate the quantitative alterations in lipid peroxidation products within the nuclei of cells from various rat tissues following the separate and combined administration of cisplatin and dexamethasone. The amount of lipid peroxidation products was determined using a spectrophotometric method, following extraction with a heptane-isopropanol mixture. When administered individually, cisplatin and dexamethasone increase the formation of lipid peroxidation products in the examined tissues of rats to varying degrees. As a result, these alterations led to corresponding changes in the oxidation index values of the analyzed nuclear preparations. During the combined administration of cisplatin and dexamethasone, some antagonistic effects were observed in the actions of these agents. Contrary to the expected synergistic enhancement of lipid peroxidation processes, a reduction in cisplatin's effect by dexamethasone was observed. Thus, it is hypothesized that such antagonistic effect of dexamethasone together with its antiinflammatory and immunomodulatory properties allows to mitigate the side effects of cisplatin. [ABSTRACT FROM AUTHOR]

The common bean (Phaseolus vulgaris L.) is an important source of protein, fiber, vitamins, and minerals, making it essential for food programs in Botswana. Prioritizing its integration into diversified farming is crucial for achieving social, environmental, and economic benefits. Previous studies primarily focused on performance under rainfed, while the effect of drought stress remains unclear. The study aimed at evaluating the effect of drought stress on four (4) genotypes: DAB541, DAB515, CAL96, and GK011, while Tepary serves as the control. The study identifies CAL96 and DAB541 genotypes as the most promising genotypes for drought tolerance as demonstrated by their increased biomass production. The increase in biomass production may be due to the overexpression of the Phaseolus vulgaris dehydration-responsive element binding (PvDREB) genes, namely PvDREB1F and PvDREB5A. Higher proline and lower malondialdehyde (MDA) levels correlated with increased catalase (CAT) and ascorbate peroxidase (APX), which are linked to hydrogen peroxide (H2O2) scavenging activity. Conversely, GK011 and DAB514 exhibited decreased dry biomass, downregulated PvDREB1F, PvDREB5A, and Pv- DREB6B, along with greater levels of MDA and H2O2 and a steady activity of APX and CAT. This suggested an enhanced membrane lipid peroxidation and a loss of membrane integrity. Navadni fižol (Phaseolus vulgaris L.) je pomemben vir beljakovin, vlaknin, vitaminov in mineralov, zaradi česar je bistven v programih prehane v Botswani. Njegovo prednostno vključevanje pri povečavanju raznolikosti kmetijske pridelave je bistveno za doseganje socialnih, okoljskih in ekonomskih ciljev. Predhodne raziskave so se prvenstveno usmerjale na njegovo uspevanje v razmerah namakanja z dežjem med tem, ko je ostajal učinek sušnega stresa nepojasnjen. V tej raziskavi so bili ovrenoteni učinki sušnega stresa na štiri genotipe in sicer DAB541, DAB515, CAL96 in GK011, pri čemer je 'Tepary' služil kot kontrola. V raziskavi sta bila genotipa CAL96 in DAB541 prepoznana kot najbolj obetajoča glede tolerance na sušo, kar se je pokazalo v njuni povečani izgradnji biomase. Povečana tvorba biomase bi lahko bila zaradi močno povečanega izražanja genov v fižolu, odzivnih na dehidracijo (PvDREB), kot sta gena PvDREB1F in PvDREB5A. Večja vsebnost prolina in manjša vsebnost malondialdehida (MDA) sta soupadali v povečanjem aktivnosti katalaze (CAT) in askorbat peroksidaze (APX), kar je povezano z odstranjevanje vodikovega peroksida (H2O2). Nasprotno sta genotipa GK011 in DAB514 pokazala zmanjšanje suhe biomase, zmanjšano izražanje PvDREB1F, PvDREB5A, in PvDREB6B genov s hkratnim povečanjem vsebnosti MDA in H2O2 in enakomerno aktivnostjo APX in CAT. To nakazuje povečano peroksidacijo membranskih lipidov in izgubo delovanja membran. [ABSTRACT FROM AUTHOR]

5α,8α-peroxyergosteryl divaricatinate (1) is isolated for the first time from acetone extract of the thallus of Cladonia cf. didyma together with condidymic (2), isodidymic (3) and barbatic (4) acids. Their UVB/UVA photoprotective and antioxidant activities were determined along with their cyto- and DNA-protecting actions against OH• damage. Compound 1 showed high UVA, cyto- (%cell viability ca. 82 at 15 µM vs 71 for OH•) and DNA (%DNA-integrity ca. 74 at 100 µM vs 22 for OH•) protecting properties. Compounds 2 and 3 exhibited high UVB (SPF ca. 40) and antioxidant activities (free radical scavenging potency EC50 = 0.6694 and 1.700 mol compound/mol DPPH•; ferric reducing power 0.392 and 0.546, at 500 ppm and inhibited lipid peroxidation); protecting cells (%cell viability ca. 76 at 15 µM) and DNA (%DNA-integrity ca. 57 at 100 µM). Compounds 1 to 3 fulfilled some physicochemical properties to act topically on the skin. [ABSTRACT FROM AUTHOR]

As a newly defined type of programmed cell death, ferroptosis is considered a potent weapon against tumors due to its distinct mechanism from other types of programmed cell death. Ferroptosis is triggered by the uncontrolled accumulation of hydroperoxyl polyunsaturated fatty acid-containing phospholipids, also called lipid peroxidation. The lipid peroxidation, generated through enzymatic and non-enzymatic mechanisms, drives changes in cell morphology and the destruction of membrane integrity. Here, we dissect the mechanisms of ferroptosis induced enzymatically or non-enzymatically, summarize the major metabolism pathways in modulating lipid peroxidation, and provide insights into the relationship between ferroptosis and tumor suppression. In this review, we discuss the recent advances of ferroptosis in tumor microenvironments and the prospect of potential therapeutic application. A review summarizes the recent advances in ferroptosis and tumor suppression, including the metabolic regulation of lipid peroxidation, the role of ferroptosis in tumor immunity response, and ferroptosis-based tumor therapeutics and clinical trials. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

As an important mollusk in reef ecosystems, Tridacna squamosa forms pro-survival symbiotic relationships that hinge on an exquisite redox equilibrium between the host and the photosynthetic symbiont, zooxanthellae. The exact regulatory mechanisms thereof remain poorly understood. In this study, a novel Nfe2-like transcription factor in T. squamosa was identified and characterized with respect to its antioxidant and cytoprotective roles. Gene structure and phylogenetic analysis reveal that T. squamosa possesses a single transcription factor TsNfe2l in contrast to mammalian Nfe2l1 (Nrf1) and Nfe2l2 (Nrf2), belonging to protein members of the bZIP-NFE2 subfamily and functionally resembling the mammalian Nfe2l1. A conserved bZIP domain permits its binding to the antioxidant response element (ARE) in vitro and in HEK293T cells. Further analyses such as promoter prediction suggest that TsNfe2l target genes engage mainly in the regulation of multiple enzymes involved in antioxidation and allied pathways. Notably, TsNfe2l transcriptionally upregulates carnosine synthase (TsATPGD), which subsequently produces L-carnosine abundantly to shield cells from oxidative damage. Moreover, the blockage of TsNfe2l nucleic acid binding reduced the expression of TsATPGD and L-carnosine content in the gill, resulting in elevated lipid peroxidation. Collectively, our findings establish novel molecular insight into TsNfe2l as a critical regulator of redox homeostasis in T. squamosa through carnosine synthesis. [ABSTRACT FROM AUTHOR]

Ischemic stroke is a devastating condition that occurs due to the interruption of blood flow to the brain, resulting in a range of cellular and molecular changes. In recent years, there has been growing interest in the role of ferroptosis, a newly identified form of regulated cell death, in ischemic stroke. Ferroptosis is driven by the accumulation of lipid peroxides and is characterized by the loss of membrane integrity. Additionally, oxidative stress, which refers to an imbalance between prooxidants and antioxidants, is a hallmark of ischemic stroke and significantly contributes to the pathogenesis of the disease. In this review, we explore the interactions between ferroptosis and oxidative stress in ischemic stroke. We examine the underlying mechanisms through which oxidative stress induces ferroptosis and how ferroptosis, in turn, exacerbates oxidative stress. Furthermore, we discuss potential therapeutic strategies that target both ferroptosis and oxidative stress in the treatment of ischemic stroke. Overall, this review highlights the complex interplay between ferroptosis and oxidative stress in ischemic stroke and underscores the need for further research to identify novel therapeutic targets for this condition. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The antioxidant activity of carnosine and of carnosine dinitrosyl iron complexes (DNICs) was studied. A system with metmyoglobin (metMb) or hemin in combination with tert-butyl hydroperoxide (t-BOOH) was used as an experimental model. Using the luminol-dependent chemiluminescence method, it was shown that carnosine and carnosine DNICs effectively diminished the level of prooxidants formed by the interaction of heme groups with t-BOOH. In addition, carnosine and carnosine DNICs inhibited the formation of diene conjugates arising during the oxidation of arachidonic acid in the metMb–t-BOOH system. In the reaction systems used, the antioxidant effect of carnosine DNICs was higher than that of carnosine. The antioxidant effect of carnosine also depended on the presence of bivalent iron ions added at a concentration equivalent to their content in DNICs. These results show that the insertion of carnosine as a ligand to nitrosyl iron complexes enhances its antioxidant properties. [ABSTRACT FROM AUTHOR]

Macrocycles bridge the gap between conventional small molecules and polymers. Drawing inspiration from successful carbon heteroatom-containing macrocycles, peroxide-containing macrocycles are gaining attention for enhanced bioactivity, potential chelating properties, and applications in energetic materials. This review presents the following strategies for the construction of cyclic peroxides with 10- to 36-membered frameworks: (1) the intramolecular iodocyclization of hydroperoxides, (2) the intermolecular cyclization of hydroperoxides with alkyl dihalides or carbonyls, (3) the acid-catalyzed rearrangements of ozonides or 11-membered cyclic triperoxides via oxy- or peroxycarbenium ions, and (4) the peroxidation of carbonyls targeting macrocyclic peroxides. The specific agents that allow for the selective construction of the medium and large cycles are also analyzed. [ABSTRACT FROM AUTHOR]

Cryopreservation is a pivotal technique in safeguarding genetic material across diverse species, despite its inherent challenges linked to induced spermatozoa damage, notably apoptosis and lipid peroxidation (LPO). Given the insufficient antioxidant defense of spermatozoa against LPO, there is a rising interest in integrating additional additives into extenders to ameliorate mammalian semen quality. Among these additives, flavonoids have garnered considerable attention due to their potent antioxidative properties. Hence, our study aimed to assess the efficacy of flavone (FL) and 3-hydroxyflavone (3-OH =) supplementation in the cryopreservation medium to protect canine sperm against the damaging impacts of freezing and ensure the preservation of their reproductive potential. Semen was collected from five Beagle stud dogs and then pooled. Then, the sample was divided into 7 groups, each treated with 1) 0 mM, 2) 0.1 mM FL, 3) 0.2 mM FL, 4) 0.4 mM FL, 5) 0.1 mM 3-OH = , 6) 0.2 mM 3-OH = , 7) 0.4 mM 3-OH =. Semen samples were subjected to cryopreservation in French straws and glycerol as a cryoprotectant. In the frozen thawed semen, sperm motility parameters by CASA system and sperm membrane integrity, acrosome status, mitochondrial activity, DNA fragmentation, early apoptosis with capacitation, and LPO were assessed using flow cytometry just after thawing (0 h) and 4 h post thaw. Results reveal significant increase in the proportion of live spermatozoa with undamaged acrosomes in the FL 0.1 and 3-OH = 0.2 groups at 0 h post thaw. At this time point, 3-OH = 0.1 significantly reduced the DNA fragmentation index (DFI) compared to the FL 0.1 and 0.2 groups. However, after the next 4 h, 3-OH = 0.4 exhibited the lowest (P < 0.05) DFI compared to FL 0.2 and 3-OH = 0.1. Additionally, 3-OH = 0.4 showed the highest (P < 0.05) proportion of non apoptotic and non capacitated spermatozoa compared to FL 0.1 0 h post-thaw. Simultaneously, the same group demonstrated significant reduction in apoptotic and capacitated sperm cells, at 0 h and 4 h post-thaw. Moreover, 3-OH = at 0.1 (0 h and 4 h) and 0.2 mM (4 h) significantly enhances the proportion of live sperm without LPO post thaw. Whitin the FL groups, only 0.4 FL significantly increased the percentage of live sperm without LPO. No significant effect of the tested substances was observed on sperm motility, cell membrane integrity, or mitochondrial activity. These findings highlight the promising role of flavone and 3-hydroxyflavone in enhancing sperm resilience during cryopreservation, suggesting their protective function against acrosome damages, capacitation, apoptosis and lipid peroxidation. [Display omitted] • lFlavone (FL) and 3-hydroxyflavone (3-OH =) were used to supplement the extender for canine semen cryopreservation for the first time. • Using FL 0.1 mM and 3-OH = 0.2 mM increased (P < 0.05) the proportion of live spermatozoa with undamaged acrosomes. • Using 3-OH = 0.1 and 0.4 mM significantly reduced the DNA fragmentation index of spermatozoa. • Using 3-OH = 0.4 mM significantly reduced apoptosis and capacitation of sperm cells. • Using FL 0.4 mM and 3-OH = 0.1 and 0.2 mM significantly protected the sperm against lipid peroxidation. [ABSTRACT FROM AUTHOR]

Ferroptosis is a form of programmed cell death characterized by iron metabolic imbalance and lipid peroxidation. Alzheimer disease (AD) is a neurodegenerative condition. Studies have shown that lipid peroxidation through ferroptosis plays a significant role in the progression of AD. Ferroptosis suppressor protein 1 (FSP1 )/coenzyme Q10 (CoQ10) signaling axis serves as a crucial regulatory element in the process of lipid peroxidation associated with ferroptosis. This review explores the mechanisms of the FSP1/CoQ10 signaling axis in AD, aiming to present a novel therapeutic approach for AD treatment. [ABSTRACT FROM AUTHOR]

Ferroptosis is implicated in viral neuropathogenesis and may underlie HIV-associated neurocognitive impairment (NCI). Emerging data also suggest differences in brain iron transport by sex. We hypothesized that circulating ferritins that inhibit ferroptosis associate with neurocognitive function and NCI in people with HIV (PWH) in a sex-biased manner. Serum ferritin heavy-chain-1 (FTH1), ferritin light-chain (FTL), and urinary F2-isoprostanes (uF2-isoPs, specific lipid peroxidation marker) were quantified in 324 PWH (including 61 women) with serial global (NPZ-4) and domain-specific neurocognitive testing. Biomarker associations with neurocognitive test scores and NCIs were evaluated by multivariable regression; correlations with uF2-isoPs were also assessed. Higher FTL and FTH1 levels were associated with less NCI in all PWH (adjusted odds ratios 0.53, 95% confidence interval (95% CI) 0.36–0.79 and 0.66, 95% CI 0.45–0.97, respectively). In women, higher FTL and FTH1 were also associated with better NPZ-4 (FTL adjusted beta (β) = 0.15, 95% CI 0.02–0.29; FTL-by-sex βinteraction = 0.32, p = 0.047) and domain-specific neurocognitive test scores. Effects on neurocognitive performance persisted for up to 5 years. Levels of both ferritins correlated inversely with uF2-isoPs in women (FTL: rho = −0.47, p < 0.001). Circulating FTL and FTH1 exert sustained, sex-biased neuroprotective effects in PWH, possibly by protecting against iron-mediated lipid peroxidation (ferroptosis). Larger studies are needed to confirm the observed sex differences and further delineate the underlying mechanisms. [ABSTRACT FROM AUTHOR]

Cisplatin (CDDP) is a platinum-based drug with anti-cancer activity and is widely used as a standard therapy for bladder cancer. It is well known that CDDP causes cell death by increasing the generation of reactive oxygen species (ROS) and lipid peroxidation, but the mechanism of its anti-cancer effects has not been fully elucidated. There are still some problems such as chemoresistance in CDDP therapy. In the present study, we found the expression of Ca2+-independent phospholipase A 2 γ (iPLA 2 γ), which has been reported to regulate cellular redox homeostasis by inhibiting lipid peroxide accumulation, in human bladder cancer tissues. Thus, we investigated the effect of iPLA 2 γ knockdown on CDDP-induced bladder cancer cell death. As a result, we found that iPLA 2 γ knockdown significantly enhanced CDDP-induced apoptosis, intracellular and mitochondrial ROS production, cytochrome c release and caspase activation in bladder cancer cells. Moreover, mitochondrial membrane potential was decreased and peroxidation of mitochondrial phospholipids was increased by iPLA 2 γ knockdown. It was also shown that co-treatment of bromoenol lactone, an iPLA 2 inhibitor, increased CDDP-induced apoptosis. These results indicated that iPLA 2 γ plays an important role in protecting bladder cancer cells from CDDP-induced apoptosis, and that iPLA 2 γ inhibitors might represent a novel strategy in CDDP-based multi-drug therapy. [Display omitted] • iPLA 2 γ knockdown enhanced CDDP-induced apoptosis of bladder cancer cells. • iPLA 2 γ knockdown accelerated CDDP-induced mitochondrial oxidative stress. • iPLA 2 γ knockdown increased CDDP-induced peroxidation of cardiolipin. • Inhibition of iPLA 2 γ might represent a novel strategy in CDDP-based drug therapy. [ABSTRACT FROM AUTHOR]

Disruption of the thyroid hormone (TH) system is connected with diverse adverse health outcomes in wildlife and humans. It is crucial to develop and validate suitable in vitro assays capable of measuring the disruption of the thyroid hormone (TH) system. These assays are also essential to comply with the 3R principles, aiming to replace the ex vivo tests often utilised in the chemical assessment. We compared the two commonly used assays applicable for high throughput screening [Luminol and Amplex UltraRed (AUR)] for the assessment of inhibition of thyroid peroxidase (TPO, a crucial enzyme in TH synthesis) using several cell lines and 21 compounds from different use categories. As the investigated cell lines derived from human and rat thyroid showed low or undetectable TPO expression, we developed a series of novel cell lines overexpressing human TPO protein. The HEK-TPOA7 model was prioritised for further research based on the high and stable TPO gene and protein expression. Notably, the Luminol assay detected significant peroxidase activity and signal inhibition even in Nthy-ori 3-1 and HEK293T cell lines without TPO expression, revealing its lack of specificity. Conversely, the AUR assay was specific to TPO activity. Nevertheless, despite the different specificity, both assays identified similar peroxidation inhibitors. Over half of the tested chemicals with diverse structures and from different use groups caused TPO inhibition, including some widespread environmental contaminants suggesting a potential impact of environmental chemicals on TH synthesis. Furthermore, in silico SeqAPASS analysis confirmed the high similarity of human TPO across mammals and other vertebrate classes, suggesting the applicability of HEK-TPOA7 model findings to other vertebrates. [ABSTRACT FROM AUTHOR]

Onions contain valuable phytochemical compounds, including quercetin derivatives. This study explores the potential of onion extract as a natural additive in chicken patties. The optimized conditions involved sonication at 80% for 5 min with a 75% ethanol concentration. The onion extract exhibited total phenolic and flavonoid compound values of 255.63 mg GAE g−1 DR and 196.87 mg QE g−1 DR, respectively. The antioxidant activity of the onion extract was characterized by an IC50 of 12.74 µg/mL. This onion extract was dominated by quercetin derivatives (quercetin 4’-O-β-glycoside and quercetin-3-O-β-glycoside and quercetin-3,4’-O-β-diglycoside). Chicken patties treated with 2% onion extract exhibited superior pH stability, lowest thiobarbituric acid reactive substances level (0.40 mg/kg) and peroxide index (0.77 mEq O2/kg meat) and maintained color stability. Comparative analysis with BHT demonstrated the efficacy of onion extract in reducing lipid oxidation. These findings highlight the potential of a 2% onion extract as effective ingredient for enhancing the quality of chicken products. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The dynamics of water physicochemical properties and hydrochemical characteristics in the Dubna River was studied from April 18, 2021 to July 4, 2021. The study showed an increase in pH with increasing water temperature and considerable variation in the concentrations of ammonium, nitrite, and phosphate ions in it. Promising approaches were shown to be the use of mathematical processing of UV-spectra of water samples by Gauss method and model biological systems based on natural lipids (low-temperature oxidation of lecithin, and its ability to spontaneously aggregate in a polar environment) for assessing the quality of natural water and its effect on the regulation of oxidation processes in biological objects. Thus, analysis of UV-spectra revealed the presence in water of hydrophobic organic compounds throughout the observation period, compounds with conjugated double bonds and N-containing compounds in late April, free fatty acids, organic compounds with a carbonyl group and P-containing compounds at the end of May and an increase in the diversity of components in water samples in late June. Water samples taken in summer, increased the rate of lecithin oxidation by a factor of more than 1.8, and, throughout the observation period, they caused staged changes in the size of aggregates formed by it and produced a considerable effect on their dzeta-potential. [ABSTRACT FROM AUTHOR]

This research aimed to study the long-term effects of soccer training on platelet membrane fatty acid levels and antioxidant vitamins. Forty-four subjects divided into soccer players (SP; n = 22; 20.86 ± 0.36 years) and a control group (CG; n = 22; 21.23 ± 0.49 years) participated in the study. The fatty acids of the platelet membrane, the rates of desaturation, lipid peroxidation indexes and intra-platelet levels of vitamins C and E were assessed. SP obtained lower values in polyunsaturated fatty acids 18:3:3 (alpha-linolenic acid), 20:5:3 (eicosapentaenoic acid) and 22:6:3 (docosahexaenoic acid) (p < 0.05). The desaturation index ∆5 was higher in SP (p < 0.05), and they had a higher lipid peroxidation index 20:4:6 (arachidonic acid)/16:0 (palmitic acid) (p < 0.05). Vitamin E and C platelet values were also higher in SP (p < 0.01). There were positive correlations in the ω6/ω3 index (p < 0.05), desaturation index ∆5 (p < 0.05), lipid peroxidation index 20:4:6/16:0 and intra-platelet vitamins E and C (p < 0.01) with the level of physical activity. In addition, there were inverse correlations in fatty acids 24:0 (lignoceric acid), 16:1 (palmitoleic acid), 20:3:6 (eicosadienoic acid) and 18:3:3 (alpha-linolenic acid) (p < 0.05) depending on the degree of physical activity. Regular long-term soccer training could modify the concentration of fatty acids such as 24:0, 16:1, 18:6, 20:3:6, 18:3:3:3, 20:5:3, 26:6:3 and ω3 PUFAs in the platelet membrane. [ABSTRACT FROM AUTHOR]

Epithelial–Mesenchymal Transition (EMT) of retinal pigment epithelial (RPE) cells is recognized as pivotal in various retinal diseases. Previous studies have suggested a reciprocal regulation between reactive oxygen species (ROS) and EMT, though the involvement of peroxidized lipids or the effects of reducing them has remained unclear. The present study disclosed that EMT of ARPE-19 cells induced by TGF-β2 and TNF-α involves increased lipid peroxidation, and Ferrostatin-1 (Fer-1), a lipophilic antioxidative agent, successfully inhibited the increase in lipid peroxidation. Fer-1 suppressed the formation of EMT-associated fibrotic deposits, while EMT induction or Fer-1 treatment did not influence the cell viability or proliferation. Functionally, Fer-1 impeded EMT-driven cell migration and reduction in transepithelial electrical resistance. It demonstrated regulatory prowess by downregulating the mesenchymal marker fibronectin, upregulating the epithelial marker ZO-1, and inhibiting the EMT-associated transcriptional factor ZEB1. Additionally, VEGF, a major pathogenic cytokine in various retinal diseases, is also upregulated during EMT, and Fer-1 significantly mitigated the effect. The present study disclosed the involvement of lipid peroxidation in EMT of RPE cells, and suggests the suppression of lipid peroxidation may be a potential therapeutic target in retinal diseases in which EMT is implicated. [ABSTRACT FROM AUTHOR]

Ferroptosis is a type of nonapoptotic cell death that is characteristically caused by phospholipid peroxidation promoted by radical reactions involving iron. Researchers have identified many of the protein factors that are encoded by genes that promote ferroptosis. Glutathione peroxidase 4 (GPX4) is a key enzyme that protects phospholipids from peroxidation and suppresses ferroptosis in a glutathione-dependent manner. Thus, the dysregulation of genes involved in cysteine and/or glutathione metabolism is closely associated with ferroptosis. From the perspective of cell dynamics, actively proliferating cells are more prone to ferroptosis than quiescent cells, which suggests that radical species generated during oxygen-involved metabolism are responsible for lipid peroxidation. Herein, we discuss the initial events involved in ferroptosis that dominantly occur in the process of energy metabolism, in association with cysteine deficiency. Accordingly, dysregulation of the tricarboxylic acid cycle coupled with the respiratory chain in mitochondria are the main subjects here, and this suggests that mitochondria are the likely source of both radical electrons and free iron. Since not only carbohydrates, but also amino acids, especially glutamate, are major substrates for central metabolism, dealing with nitrogen derived from amino groups also contributes to lipid peroxidation and is a subject of this discussion. [ABSTRACT FROM AUTHOR]

Nitrosyl iron complexes are remarkably multifactorial pharmacological agents. These compounds have been proven to be particularly effective in treating cardiovascular and oncological diseases. We evaluated and compared the antioxidant activity of tetranitrosyl iron complexes (TNICs) with thiosulfate ligands and dinitrosyl iron complexes (DNICs) with glutathione (DNIC-GS) or phosphate (DNIC-PO4−) ligands in hemoglobin-containing systems. The studied effects included the production of free radical intermediates during hemoglobin (Hb) oxidation by tert-butyl hydroperoxide, oxidative modification of Hb, and antioxidant properties of nitrosyl iron complexes. Measuring luminol chemiluminescence revealed that the antioxidant effect of TNICs was higher compared to DNIC-PO4−. DNIC-GS either did not exhibit antioxidant activity or exerted prooxidant effects at certain concentrations, which might have resulted from thiyl radical formation. TNICs and DNIC-PO4− efficiently protected the Hb heme group from decomposition by organic hydroperoxides. DNIC-GS did not exert any protective effects on the heme group; however, it abolished oxoferrylHb generation. TNICs inhibited the formation of Hb multimeric forms more efficiently than DNICs. Thus, TNICs had more pronounced antioxidant activity than DNICs in Hb-containing systems. [ABSTRACT FROM AUTHOR]

Copyright of Retos: Nuevas Perspectivas de Educación Física, Deporte y Recreación is the property of Federacion Espanola de Asociaciones de Docentes de Educacion Fisica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ferroptosis, an iron-dependent form of regulated cell death, has recently emerged as a crucial process in the pathogenesis of Pyricularia oryzae, the causal agent of the devastating rice blast disease, which causes billions of dollars in annual losses. This mini review explores the potential of antioxidants in suppressing ferroptosis in P. oryzae to promote sustainable rice production, with significant implications for global food security and nutrition. We critically analyze the current literature on the mechanisms of ferroptosis in P. oryzae, including iron metabolism and lipid peroxidation, the role of different antioxidants in inhibiting this cell death pathway, and the potential applications of antioxidant-based strategies for the management of rice blast disease. Recent discoveries, such as the efficacy of the natural flavonoid tangeretin in inhibiting fungal ferroptosis by interfering with the accumulation of iron and reactive oxygen species, highlight the promise of natural and nature-inspired compounds for disease management. The use of antioxidants to modulate ferroptosis in P. oryzae offers several advantages over traditional fungicide-based approaches, including improved safety, sustainability, and potential nutritional benefits through antioxidant-enriched rice varieties. However, challenges such as optimizing delivery methods, managing potential resistance, and ensuring efficacy under different environmental conditions need to be addressed. To achieve these goals, future research should focus on identifying the most effective antioxidant compounds, exploring synergistic combinations, and developing sustainable application methods.

The kinetics of change in peroxide value and weight gain were simultaneously studied during the peroxidation of three vegetable oils of various chemical compositions. The initiation and propagation oxidizability parameters Oi (kg mEq−1 h2) and rn (h−1) of the canola, corn, and peanut oils were calculated to be 412 and 0.0894, 160 and 0.0641, and 36.1 and 0.0304, respectively. The weight-based parameter Oiw ranked the oils (103,000, 51,000, and 8000% h2, respectively) as the parameter Oi did. However, the equivalent parameter rnw (0.0680, 0.1198, and 0.1875 h−1, respectively) provided a reverse order than that of the parameter rn. This was attributed to the fact that the value of rn represents the formation and decomposition of lipid hydroperoxides in general, whereas the value of rnw actually represents the overall tendency of an oil to the formation and polymerization of lipid hydroperoxides.

Critical seagrass ecosystems are predicted to be impacted by droughts and floods of increasing severity and frequency as the climate changes. Hyposalinity events alter seagrass composition and challenge resilience, yet understanding of the response mechanisms is incomplete but necessary for predicting outcomes. Hyposalinity stress response mechanisms in Halodule wrightii (shoal grass), a seagrass with wide salinity tolerance, were examined in mesocosm experiments by measuring monovalent and divalent ions, free amino acid (FAA) concentrations, and the Thiobarbituric Acid Reactive Substances (TBARS) indicator of lipid peroxidation. Two progressive, 3-step treatments, to moderate and extreme hyposalinity endpoints S15 and S5 were compared. Concentrations of Na+, K+, Ca2+, and Cl− decreased two to threefold with greater hyposalinity. Proline, the most abundant FAA initially, progressively decreased with increasing hyposalinity as asparagine became dominant. Glutamine and serine increased significantly, and all four FAA exhibited consistent patterns of change. Results are consistent with proline's role as osmolyte and regulator, and suggest that asparagine mobilizes or stores N, possibly due to another limiting nutrient. TBARS changed transiently at intermediate salinities, but were not significantly different at hyposalinity endpoints indicating that H. wrightii may have already reached stress equilibrium. Prolonged hyposalinity events may have consequences for seagrass resilience. [ABSTRACT FROM AUTHOR]

Excessive fat deposition due to impaired fat metabolism in chickens is a major problem in the poultry industry. Nutritional interventions are effective solutions, but current options are limited. A safe phytochemical, rutin, has shown positive effects in animals, but its effect on lipid metabolism in poultry remains unknown. Hence, this study is to investigate the effects of rutin on egg quality, serum biochemistry, fat deposition, lipid peroxidation and hepatic lipid metabolism in post-peak laying hens. A total of 360 Taihang laying hens (49-week-old) were randomly divided into five groups and fed a basal diet (control group, 0%) and a basal diet supplemented with 300 (0.03%), 600 (0.06%), 900 (0.09%), and 1,200 (0.12%) mg rutin/kg feed, respectively. The results showed that eggshell strength was significantly (p < 0.05) higher in the dietary rutin groups, whereas yolk percentage (p < 0.05), total cholesterol (TC) (p < 0.01) and yolk fat ratio (p < 0.01) decreased linearly (p < 0.05) in the dietary rutin groups. Importantly, dietary rutin reduced serum triglyceride (TG) and TC levels, decreased abdominal lipid deposition and liver index (p < 0.05), and which concomitantly decreased hepatic lipid (TG, TC, and free fatty acid) accumulation (p < 0.05). An increase (p < 0.05) in total antioxidant capacity and superoxide dismutase activity and a decrease (p < 0.05) in malondialdehyde levels were also found. At the same time, the activities of hepatic lipase, acetyl-CoA carboxylase and malic enzyme in the liver were decreased (p < 0.05). Dietary rutin also increased (p < 0.05) the expression of fatty acid oxidation-related genes (carnitine palmitoyl transferase 1, peroxisome proliferator-activated receptor α, farnesoid X receptor). Additionally, it decreased fatty acid synthesis genes (sterol regulatory element binding protein-1c, acetyl-CoA carboxylase α, stearoyl-CoA desaturase 1) (p < 0.05). In conclusion, the addition of rutin (0.06-0.12%) to the diet improved the fat metabolism and increased liver antioxidant capacity in post-peak laying hens, and these positive changes improved egg quality to some extent. [ABSTRACT FROM AUTHOR]