234 results on '"Perrin, Florence"'
Search Results
2. Advances in spinal cord injury: insights from non-human primates
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Poulen, Gaetan, primary and Perrin, Florence E., additional
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- 2024
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3. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord
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Bauchet, Luc, primary, Poulen, Gaetan, additional, Lonjon, Nicolas, additional, Vachiery-Lahaye, Florence, additional, Bourinet, Emmanuel, additional, Perrin, Florence Evelyne, additional, and Hugnot, Jean-Philippe, additional
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- 2021
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4. CRISPR, Prime Editing, Optogenetics, and DREADDs: New Therapeutic Approaches Provided by Emerging Technologies in the Treatment of Spinal Cord Injury
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Paschon, Vera, Correia, Felipe Fernandes, Morena, Beatriz Cintra, da Silva, Victor Allisson, dos Santos, Gustavo Bispo, da Silva, Maria Cristina Carlan, Cristante, Alexandre Fogaça, Willerth, Stephanie Michelle, Perrin, Florence Evelyne, and Kihara, Alexandre Hiroaki
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- 2020
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5. CSF1R inhibition at chronic stage after spinal cord injury modulates microglia proliferation.
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Perez, Jean‐Christophe, Poulen, Gaetan, Cardoso, Maida, Boukhaddaoui, Hassan, Gazard, Chloé Marie, Courtand, Gilles, Bertrand, Sandrine Sylvie, Gerber, Yannick Nicolas, and Perrin, Florence Evelyne
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- 2023
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6. In vivo astrocyte reprogramming following spinal cord injury
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Gerber, Yannick N., primary and Perrin, Florence E., additional
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- 2023
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7. A Novel Translational Model of Spinal Cord Injury in Nonhuman Primate
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Le Corre, Marine, Noristani, Harun N., Mestre-Frances, Nadine, Saint-Martin, Guillaume P., Coillot, Christophe, Goze-Bac, Christophe, Lonjon, Nicolas, and Perrin, Florence E.
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- 2018
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8. Up-Regulation of Astrocytic Fgfr4 Expression in Adult Mice after Spinal Cord Injury
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Bringuier, Claire Mathilde, primary, Noristani, Harun Najib, additional, Perez, Jean-Christophe, additional, Cardoso, Maida, additional, Goze-Bac, Christophe, additional, Gerber, Yannick Nicolas, additional, and Perrin, Florence Evelyne, additional
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- 2023
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9. Organization of Collagen I Fibers and Tissue Hardening: Markers of Fibrotic Scarring after Spinal Cord Injury in Mice Revealed by Multiphoton-Atomic Force Microscopy Imaging
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Manesco, Clara, primary, Saavedra-Villanueva, Oscar, additional, Martin, Marta, additional, de Lizaraga, Joshua, additional, Varga, Bela, additional, Cloitre, Thierry, additional, Gerber, Yannick Nicolas, additional, Perrin, Florence Evelyne, additional, and Gergely, Csilla, additional
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- 2023
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10. UV degradation of clay-reinforced polypropylene nanocomposites
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Salah, Hend Ben Hadj, Daly, Hachmi Ben, Denault, Johanne, and Perrin, Florence
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Ultraviolet radiation -- Usage ,Photodegradation -- Observations ,Polypropylene -- Chemical properties -- Optical properties ,Reinforced plastics -- Chemical properties -- Optical properties ,Engineering and manufacturing industries ,Science and technology - Abstract
The aim of this work is to experimentally characterize the UV-degradation process at both the surface and at different layers across the thickness of injection-molded polypropylene (PP) matrix containing different amounts of nanosized montmorillonite (MMT) clay particles. These nanocomposite materials have been exposed to UV irradiations (λ = 320 nm) at different preset temperatures (25, 45, and 65°C) in the presence of oxygen and during different exposure times. The extent of such process at these layers was determined using both the FTIR spectroscopy and the wide-angle X-ray diffraction analyses. The micromechanical properties across the thickness have been characterized using the nanoindentation technique. The obtained results have indicated that the UV-degradation process for the nanocomposite materials is much more intense than the one observed for the neat PP. Moreover, it has been noted that such degradation process is not uniform across the thickness of the exposed materials. Results obtained from the X-ray analysis have shown an increase of the crystallinity of the polymer molecules at only the external surface of the exposed materials. This was confirmed using the nanoindentation test as an increase of the Young's modulus at this layer was noted., INTRODUCTION Polymer nanocomposites are polymers that have been reinforced with small quantities (less than 5% by weight) of nanosized particles (nanofillers) having high aspect ratios (L/D > 300) [1]. These [...]
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- 2016
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11. Monitoring spinal cord injury in mice with a multimodal label-free imaging approach
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Clara Manesco, Joshua de Lizaraga, Béla Varga, cloitre thierry, Marta Martin, Yannick Gerber, Perrin, Florence E., and Csilla Gergely
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- 2022
12. Monitoring spinal cord injury in mice with a multimodal label-free imaging approach
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Manesco, Clara, primary, de Lizaraga, Joshua, additional, Varga, Bela, additional, Cloitre, Thierry, additional, Martin, Marta, additional, Gerber, Yannick, additional, Perrin, Florence, additional, and Gergely, Csilla, additional
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- 2022
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13. Chemicals from agricultural biomass: chemoenzymatic approach for production of vinylphenols and polyvinylphenols from phenolic acids
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Leisch Hannes, Grosse Stephan, Morley Krista, Abokitse Kofi, Perrin Florence, Denault Johanne, and Lau Peter C.K.
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biorefinery ,biotransformation ,chemoenzymatic synthesis ,green polymers ,phenolic acids ,Chemistry ,QD1-999 - Abstract
A two-step chemoenzymatic process for the preparation of polyvinylphenols from phenolic acids (PAs), being abundant aromatic constituents found in agricultural biomass, was developed. In the first step, conversion of 4-hydroxycinnamic acid derivatives to the corresponding vinylphenols, mediated by a recombinant phenolic acid decarboxylase, was evaluated using a variety of bioprocessing technologies that include biphasic whole cell and cell free extract biotransformations, a combination of biocatalyst with adsorbent resins for in situ product recovery, and fixed bed reactors using immobilized whole cells. The best yield (90%) with a high space time yield of 4.83 g/l/h was the result of a combination of crude enzyme extracts of the recombinant Escherichia coli (E. coli) with water immiscible organic solvents such as toluene. In the second step, cationic and radical polymerizations were tested to produce polyvinylguaiacol (PVG) from vinyl phenols. Characterization of PVG by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and nanoindentation test are reported here for the first time. The feasibility of the chemoenzymatic process for the production of aromatic polymers from biomass was demonstrated by the production of polymers from a mixture of ferulic acid (FA) and p-coumaric acid (pCA), obtained from alkaline hydrolysis of corn bran. Interestingly, nanoindentation tests showed that both PVG and “mixed” PVG polymers showed significantly higher performances than a commercial polystyrene polymer.
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- 2013
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14. Comparing behavioral responses across multiple assays of stress and anxiety in zebrafish (Danio rerio)
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Wong, Ryan Y., Perrin, Florence, Oxendine, Sarah E., Kezios, Zachary D., Sawyer, Susanna, Zhou, Linran, Dereje, Simone, and Godwin, John
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- 2012
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15. Astrocyte‐targeting RNA interference against mutated superoxide dismutase 1 induces motoneuron plasticity and protects fast‐fatigable motor units in a mouse model of amyotrophic lateral sclerosis
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Rochat, Cylia, primary, Bernard‐Marissal, Nathalie, additional, Källstig, Emma, additional, Pradervand, Sylvain, additional, Perrin, Florence E., additional, Aebischer, Patrick, additional, Raoul, Cédric, additional, and Schneider, Bernard L., additional
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- 2022
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16. Unlike Brief Inhibition of Microglia Proliferation after Spinal Cord Injury, Long-Term Treatment Does Not Improve Motor Recovery
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Poulen, Gaëtan, primary, Bartolami, Sylvain, additional, Noristani, Harun N., additional, Perrin, Florence E., additional, and Gerber, Yannick N., additional
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- 2021
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17. Multimodal label-free imaging approach to monitor spinal cord injury in mice
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Manesco, Clara, de Lizaraga, Joshua, Varga, Béla, Cloitre, Thierry, MARTIN FERNANDEZ, Marta, Gerber, Yannick, Perrin, Florence, Gergely, Csilla, Laboratoire Charles Coulomb (L2C), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
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[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] - Abstract
International audience; Aim: With the objective to investigate the mechanisms underlying absence of spontaneous axonal regeneration following spinal cord injury (SCI) we employ a multimodal label-free imaging approach to monitor glial scar in a mice SCI model. Method: To determine the relevant structural signature, and the nanobiomechanical behavior of healthy and injured spinal cord tissue we combine the non-linear, multiphoton microscopy (MPM) technique with force measurements via atomic force microscopy (AFM). The glial scar at different post lesion time-points is investigated with these two techniques to monitor structural and elasticity (Young modulus) changes of the tissue.Results: 2-photon excited fluorescence (2PEF) and second harmonic generation (SHG) signals of excised mice SC injured tissues were recorded in MPM at 72h, 1week and 6 weeks post-lesion. The MPM images revealed the apparition of a strong SHG signal at 1week post injury, due to the formation of fibrillar collagen fibers (collagen type I) by the injury site in the glial scar. At 6 weeks’post-injury, the SHG signal is more intense and a higher number of fibers are detected in average. We further assessed the preferential orientation of the collagen bundles performing polarization dependent measurements of the SHG signal. The AFM based force spectroscopy measurements have been performed at the same post-lesion time-points to map the elastic properties of the healthy (grey and white matters) and injured (lesion) parts in the spinal cord tissue. The results suggested an increase of the lesion area stiffness over time that could be correlated with the apparition of fibrillar collagen observed in MPM, indicating the presence of a fibrotic process seven days after injury, that develops in time. As tissue stiffness is a regulator of neuronal growth, such kind of measurements might help to understand why adult mammalian axons do not regenerate after an injury. Our next step is to investigate the effect of a treatment (pharmacological transient depletion of microglia/macrophage in mice that underwent SCI) on the structure and mechanical properties of the lesion site at 1 week and 6 weeks post injury.
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- 2021
18. Non-isothermal crystallization behavior of clay-reinforced polypropylene nanocomposites
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Salah Hend Ben Hadj, Ben Daly Hachmi, Perrin Florence, and Denault Johanne
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activation energy ,montmorillonite ,nanocomposite ,non-isothermal crystallization ,polypropylene ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
In this study, the non-isothermal crystallization kinetics of polypropylene (PP) in the presence of nanoclay particles were investigated using differential scanning calorimetry (DSC) with various cooling rates varying from 0.5°C/min to 80°C/min. Such kinetics were compared with those obtained for the pure PP matrix. The modified Avrami analysis was used to describe the non-isothermal crystallization process. The results obtained indicate that the presence of nanoclay significantly affected the crystallization rate of the PP resin, since an increase of the crystallization temperature as the nanoclay content increased was observed. This was attributed to the nucleating ability of these particles. Moreover, it was seen that the increase of both the cooling rate and the nanoclay content decreased the Avrami exponent n, suggesting a change in the obtained crystalline shape. For the nanocomposite materials, as well as for neat PP, the mechanism of crystallization was found to undergo two transitions, at about 5°C/min and 40°C/min. This suggests that the surface-induced nucleation at the clay particles follows the same mechanisms as those of the complete spherulitic structure. However, a lower value of activation energy for crystallization was obtained as the clay content increased, confirming the nucleating effect of clay particles.
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- 2011
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19. RNA Profiling of Mouse Ependymal Cells after Spinal Cord Injury Identifies the Oncostatin Pathway as a Potential Key Regulator of Spinal Cord Stem Cell Fate
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Chevreau, Robert, primary, Ghazale, Hussein, additional, Ripoll, Chantal, additional, Chalfouh, Chaima, additional, Delarue, Quentin, additional, Hemonnot-Girard, Anne Laure, additional, Mamaeva, Daria, additional, Hirbec, Helene, additional, Rothhut, Bernard, additional, Wahane, Shalaka, additional, Perrin, Florence Evelyne, additional, Noristani, Harun Najib, additional, Guerout, Nicolas, additional, and Hugnot, Jean Philippe, additional
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- 2021
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20. Dynamic Diversity of Glial Response Among Species in Spinal Cord Injury
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Perez, Jean-Christophe, primary, Gerber, Yannick N., additional, and Perrin, Florence E., additional
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- 2021
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21. 662 Dissecting the CD226 immune axis in the tumor microenvironment using CyTOF-based high-dimensional immunophenotyping
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Vowell, Katie, primary, Conner, Michael, additional, Perrin, Florence, additional, Bojczuk, Paul, additional, Hance, Kenneth, additional, Roth, Iris, additional, Donahue, Christine, additional, Smothers, James, additional, and Waight, Jeremy, additional
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- 2021
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22. Coherent Anti-Stokes Raman Scattering Microscopy: A Label-Free Method to Compare Spinal Cord Myelin in Different Species
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Poulen, Gaëtan, primary, Gerber, Yannick N, additional, Perez, Jean-Christophe, additional, Oubarrahou, Khadidja, additional, Lonjon, Nicolas, additional, Vachiery-Lahaye, Florence, additional, Boukhaddaoui, Hassan, additional, and Perrin, Florence E., additional
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- 2021
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23. Label-free imaging techniques for monitoring spinal cord injury: from pathophysiology to therapeutic strategies in mice
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Manesco, Clara, de Lizaraga, Joshua, Varga, Béla, Cloitre, Thierry, Martin-Fernandez, Marta, Gerber, Yannick, Perrin, Florence, Gergely, Csilla, Laboratoire Charles Coulomb (L2C), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
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[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] - Abstract
International audience; Spinal cord injuries (SCI) affect between 2.5 and 4 million patients worldwide yielding major handicaps and inducing high economical costs. A scar, called glial scar and composed of two main cellular populations i.e. astrocytes and microglia, inhibits axonal regeneration by forming a physical and chemical barrier. Currently, there is no curative treatment on any symptoms associated with SCI. In this context, with the objective to investigate the mechanisms underlying absence of spontaneous axonal regeneration following SCI, we employ a multimodal label-free imaging approach to monitor glial scar in a mice SCI model. Method: To determine the relevant structural signature and the nanobiomechanical behavior of healthy and injured spinal cord tissue, we combine the non-linear, multiphoton microscopy (MPM) technique with force measurements via atomic force microscopy (AFM). The glial scar at different key post lesion time-points is investigated with these two techniques to monitor structural and elasticity (Young modulus) changes of the tissue.Results: 2-photon excited fluorescence (2PEF) and second harmonic generation (SHG) signals of excised mice SC injured tissues were recorded in MPM at 72h,1 week and 6 weeks post-lesion. The MPM images revealed the apparition of a strong SHG signal at 1week post injury, due to the formation of fibrillar collagen fibers (collagen type I) by the injury site in the glial scar. At 6 weeks post-injury, the SHG signal is more intense and a higher number of fibers are detected in average. We further assessed the preferential orientation of the collagen bundles performing polarization dependent measurements of the SHG signal. The AFM based force spectroscopy measurements have been performed at the same post-lesion time-points to map the elastic properties of the healthy (grey and white matters) and injured (lesion) parts in the spinal cord tissue. The results suggested an increase of the lesion area stiffness over time that could be correlated with the apparition of fibrillar collagen observed in MPM, indicating the presence of a fibrotic process seven days after injury, that develops in time. As tissue stiffness is a regulator of neuronal growth, such kind of measurements might help to understand why adult mammalian axons do not regenerate after an injury. Our next step is to investigate the effect of a treatment (pharmacological transient depletion of microglia/macrophage in mice that underwent SCI) on the structure and mechanical properties of the lesion site at 2 weeks and 6 weeks post injury.
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- 2021
24. An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
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Bonsignori, Mattia, Wiehe, Kevin, Grimm, Sebastian K., Lynch, Rebecca, Yang, Guang, Kozink, Daniel M., Perrin, Florence, Cooper, Abby J., Hwang, Kwan-Ki, Chen, Xi, Liu, Mengfei, McKee, Krisha, Parks, Robert J., Eudailey, Joshua, Wang, Minyue, Clowse, Megan, Criscione-Schreiber, Lisa G., Moody, M. Anthony, Ackerman, Margaret E., Boyd, Scott D., Gao, Feng, Kelsoe, Garnett, Verkoczy, Laurent, Tomaras, Georgia D., Liao, Hua-Xin, Kepler, Thomas B., Montefiori, David C., Mascola, John R., and Haynes, Barton F.
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Autoantibodies -- Identification and classification -- Physiological aspects -- Research ,Systemic lupus erythematosus -- Genetic aspects -- Research ,Immunological tolerance -- Research ,HIV infection -- Genetic aspects -- Research ,Health care industry - Abstract
Broadly HIV-1-neutralizing antibodies (BnAbs) display one or more unusual traits, including a long heavy chain complementarity-determining region 3 (HCDR3), polyreactivity, and high levels of somatic mutations. These shared characteristics suggest that BnAb development might be limited by immune tolerance controls. It has been postulated that HIV-1-infected individuals with autoimmune disease and defective immune tolerance mechanisms may produce BnAbs more readily than those without autoimmune diseases. In this study, we identified an HIV-1-infected individual with SLE who exhibited controlled viral load (< 5,000 copies/ml) in the absence of controlling HLA phenotypes and developed plasma HIV-1 neutralization breadth. We collected memory B cells from this individual and isolated a BnAb, CH98, that targets the CD4 binding site (CD4bs) of HIV-1 envelope glycoprotein 120 (gp120). CH98 bound to human antigens including dsDNA, which is specifically associated with SLE. Anti-dsDNA reactivity was also present in the patient's plasma. CH98 had a mutation frequency of 25% and 15% nt somatic mutations in the heavy and light chain variable domains, respectively, a long HCDR3, and a deletion in the light chain CDR1. The occurrence of anti-dsDNA reactivity by a HIV-1 CD4bs BnAb in an individual with SLE raises the possibility that some BnAbs and SLE-associated autoantibodies arise from similar pools of B cells., Introduction Broadly HIV-1-neutralizing antibodies (BnAbs) have been isolated that bind to multiple epitopes on the envelope glycoproteins gp120 and gp41 (reviewed in ref. 1). 2G12 recognizes a posttranslational glycan epitope [...]
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- 2014
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25. Multimodal imaging approach for optical label-free readout of spinal cord injury
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Manesco, Clara, Varga, Béla, Cloitre, Thierry, MARTIN FERNANDEZ, Marta, Gerber, Yannick, Perrin, Florence, Gergely, Csilla, Laboratoire Charles Coulomb (L2C), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
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[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] - Abstract
International audience; Spinal cord injuries (SCI) affect between 2.5 and 4 million patients worldwide (40 000 in France). Handicaps induced by SCI range from minimal sensory motor deficits to complete tetraplegia. Currently, there is no curative treatment on any symptoms associated with SCI. A scar, called glial and composed mainly of astrocytes and microglia, appears at the the injury site and inhibits axonal regeneration. This mechanism is poorly understood. Better monitoring of animal models of SCI in particular through the development of bioimaging translational tools are therefore mandatory.Our main objectives are to pursue and extend a multimodal imaging approach combining multiphoton spectroscopy (MPM), Atomic Force Microscopy (AFM) and Broadband coherent anti-Stokes Raman Spectroscopy (BCARS), a novel technique [1] based on Raman spectroscopy with faster image acquisitions [2], to better understand mechanisms that underlie the glial scar formation and the subsequent absence of spontaneous axonal regeneration following SCI, as well as to evaluate outcomes of therapeutic strategies that favor axonal regeneration in mice and non-human primates. MPM, conceived to specifically reveal non centro-symmetric structures with second harmonic generation (SHG) [3], provided a first evidence of the presence of collagen fibers as a biomarker of the injury site. AFM showed significant differences in Young Modulus between healthy and injured areas. These results must be supplemented with a deeper collagen characterization and axons imaging using MPM, as well as a treatment monitoring by co-localizing drugs within healthy/diseased tissues using BCARS [4].[1]. C. H. Camp Jr, Y. J. Lee, J. M. Heddleston, C. M. Hartshorn, A. R. Hight Walker, J. N. Rich, J. D. Lathia, M. T. Cicerone, Nat. Photonics 2014, 8, 627.[2]. C. M. Hartshorn, Y. J. Lee, C. H. Camp Jr, Z. Liu, J. M. Heddleston, N. Canfield, T. A. Rhodes, A. R. Hight Walker, P. J. Marsac, M. T. Cicerone, Anal. Chem. 2013, 85, 8102[3]. L. Mostaço-Guidolin, N.L. Rosin, T.L. Hackett, Int. J. Mol. 2017, 18(8), 1772. [4]. C. Zhang, D. Zhang, J.-X. Cheng, Annu. Rev. Biomed. Eng. 2015, 17, 415. [4]. C. Zhang, D. Zhang, J.-X. Cheng, Annu. Rev. Biomed. Eng. 2015, 17, 415.
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- 2021
26. Non-linear optical imaging and atomic force measurements to monitor spinal cord injury in mice and non-human primates
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Manesco, Clara, Varga, Béla, Gerber, Yannick, Cloitre, Thierry, MARTIN FERNANDEZ, Marta, Gergely, Csilla, Perrin, Florence, Laboratoire Charles Coulomb (L2C), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
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[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] - Abstract
International audience; Aim: Our main objective is to implement a multimodal (bio)physical approach to decipher mechanisms that underlie absence of spontaneous axonal regeneration following spinal cord injury (SCI) in mice and nonhuman primates. Method: A multimodal imaging strategy is employed combining state-of-the-art non-linear microscopy techniques (multiphoton - MPM and BCARS microscopy) with force spectroscopy measurements via atomic force microscopy (AFM). This approach enables to assess the relevant structural, morphological and spectral signature as well as the nanobiomechanical behavior of healthy and injured spinal cord tissue at cellular level. Results: We have first imaged excised mice SC tissues in 2-photon excited fluorescence and second harmonic generation (SHG). These MPM studies revealed the apparition of a strong SHG signal due to the formation of collagen I fibers by the injury site in the glial scar. AFM based force spectroscopy measurements have been also performed to map the elastic properties of the healthy and injured spinal cord tissue. Therefore, MPM data could be correlated with Young modulus elasticity results, indicating the presence of a fibrotic process 7 days after injury. As tissue stiffness is a regulator of neuronal growth, such kind of measurements will enable to understand why adult mammalian axons do not regenerate spontaneously after injury. Our next step will be to use a novel, emerging imaging technology that is the broadband coherent anti-Stokes Raman scattering (BCARS) microscopy for fast, label-free, highly selective and specific screening of spinal cord injury and potentially of drugs uptake into excised tissues. Combination of BCARS and MPM will first enable to retrieve information on the biological state of the cells/tissues that may underly the absence of spontaneous axonal regeneration following SCI (Raman neuronal markers: myelin, acetylcholine neurotransmitter, glutamate; MPM markers: collagen, neural axons, mitochondria,…). Second, it will permit to further correlate modification of these structural, morphological and spectral signatures in the context of therapeutic strategies.
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- 2021
27. Morphological aspects of injected polypropylene/clay nanocomposite materials
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Salah, Hend Ben Hadj, Daly, Hachmi Ben, Denault, Johanne, and Perrin, Florence
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Polypropylene -- Mechanical properties ,Polymeric composites -- Mechanical properties ,Polymers -- Rheology ,Clay -- Mechanical properties ,Engineering and manufacturing industries ,Science and technology - Abstract
Polypropylene (PP) clay nanocomposites were injection-molded using two different coupling agents based on maleic anhydride-grafted PP (MA-g-PP) and two clay loadings. The morphological aspects of these materials were studied by depth profiling. Molecular chain and clay orientations were characterized using attenuated total reflectance-infrared analysis and transmission electron microscopy (TEM). Both clay platelets and PP molecular chain orientations were found to decrease from the surface toward the core of the injection-molded specimens. Clay intercalation, characterized by both complementary X-ray diffraction and TEM, was found to be significantly influenced by both the characteristics of the coupling agent used and the type of residual stresses generated at each layer across the thickness of the injection-molded parts. The use of low-molecular weight ([M.sub.w]) MA-g-PP led to a uniform intercalation but with no further exfoliation. The use of higher molecular weight MA-g-PP led to a heterogeneous intercalation with some signs of exfoliation. The crystallization behavior of PP clay nanocomposites studied by differential scanning calorimetry showed an increase in the level of crystallinity from the surface to the core of the specimens; these results were also confirmed by scanning electron microscopy. POLYM. ENG. SCI., 53:905-913, 2013. © 2012 Society of Plastics Engineers, INTRODUCTION Polymer nanocomposites are polymers that have been reinforced with small quantities (less than 5% by weight) of nanosized particles (nanofillers), having high-aspect ratios (L/D > 300) (1). These materials [...]
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- 2013
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28. Inhibiting microglia proliferation after spinal cord injury improves recovery in mice and nonhuman primates
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Poulen, Gaëtan, primary, Aloy, Emilie, additional, Bringuier, Claire M., additional, Mestre-Francés, Nadine, additional, Artus, Emaëlle V.F., additional, Cardoso, Maïda, additional, Perez, Jean-Christophe, additional, Goze-Bac, Christophe, additional, Boukhaddaoui, Hassan, additional, Lonjon, Nicolas, additional, Gerber, Yannick N., additional, and Perrin, Florence E., additional
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- 2021
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29. RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells
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Ghazale, Hussein, Ripoll, Chantal, Leventoux, Nicolas, Jacob, Laurent, Azar, Safa, Mamaeva, Daria, Glasson, Yael, Calvo, Charles-Félix, Thomas, Jean-Léon, Meneceur, Sarah, Lallemand, Yvan, Rigau, Valérie, Perrin, Florence, Noristani, Harun, Rocamonde, Brenda, Huillard, Emmanuelle, Bauchet, Luc, Hugnot, Jean-Philippe, Hôpital Saint Eloi (CHRU Montpellier), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Sorbonne Université (SU), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Yale School of Medicine [New Haven, Connecticut] (YSM), Institut Pasteur [Paris] (IP), Cellules Souches et Développement / Stem Cells and Development, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), CHU Montpellier, Université de Montpellier (UM), This work was supported by grants from IRP (Switzerland), IRME (France), AFM (France), ANR EU ERANET Neuroniche (J.-P.H.), and ANR Brainwash (J.-L.T., L.J.). H.G. was supported by an AFM PhD fellowship, We thank all Montpellier biocampus facilities for help (RHEM, MRI RIO, RAM) and excellent technical work. We are very grateful to Dr. H Boukhaddaoui (imaging), Dr. C Duperray (cytometry), M. Maistre (laser microdissection facilities, Bordeaux), V. Pantesco (Affymetrix facilities), and M. Goussard/P. Guigue (animals) for providing technical expertise in this work. We warmly thank Prof. Morohashi (Kyushu University, Japan) for anti-Arx antibody. The authors declare that there is no conflict of interest regarding the publication of this article., ANR-17-CE14-0005,BrainWash,Drainage lymphatique, recirculation immunitaire et réparation neuronale après un accident cérébrovasculaire(2017), ANR-16-NEU3-0001,NEURONICHE,Spinal cord repair from endogenous stem cells in the spinal niche(2016), Gestionnaire, Hal Sorbonne Université, Drainage lymphatique, recirculation immunitaire et réparation neuronale après un accident cérébrovasculaire - - BrainWash2017 - ANR-17-CE14-0005 - AAPG2017 - VALID, Spinal cord repair from endogenous stem cells in the spinal niche - - NEURONICHE2016 - ANR-16-NEU3-0001 - ERANET NEURON III - VALID, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Perrin, Florence, Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), CHU Saint-Eloi, Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Yale University School of Medicine, Institut Pasteur [Paris], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
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Resource ,Male ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,Ependymoglial Cells ,radial glial cells ,floor plate ,Mice, Transgenic ,Mice ,Young Adult ,transcription factors ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Animals ,Humans ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Stem Cell Niche ,lcsh:QH301-705.5 ,ComputingMilieux_MISCELLANEOUS ,Embryonic Stem Cells ,ependyma ,neural stem cells ,MSX1 Transcription Factor ,lcsh:R5-920 ,roof plate ,Gene Expression Profiling ,Stem Cells ,ependymal cells ,[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,Gene Expression Regulation, Developmental ,spinal cord ,Middle Aged ,niche ,lcsh:Biology (General) ,Microscopy, Fluorescence ,regionalization ,RNA ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Female ,lcsh:Medicine (General) ,Msx1 - Abstract
Summary: Anamniotes, rodents, and young humans maintain neural stem cells in the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120 transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental transcription factors (ARX, FOXA2, MSX1, and PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and floor plates. The dorsal EZ expresses a high level of Bmp6 and Gdf10 genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4 transcription factors. : A niche of stem cells is present around the central canal of the adult spinal cord. A better description of cell diversity and genes expressed in this niche may help to use it to promote spinal cord regeneration after lesions. In this article, based on several techniques, Ghazale and colleagues provide a cellular and molecular resource for the adult human and mouse stem cell niches. Keywords: spinal cord, niche, neural stem cells, regionalization, ependyma, ependymal cells, radial glial cells, transcription factors, Msx1, roof plate, floor plate
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- 2019
30. Negative Impact of Sigma-1 Receptor Agonist Treatment on Tissue Integrity and Motor Function Following Spinal Cord Injury
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Lattard, Alise, primary, Poulen, Gaëtan, additional, Bartolami, Sylvain, additional, Gerber, Yannick N., additional, and Perrin, Florence E., additional
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- 2021
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31. The seaweeds of South Australia /
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Lucas, A H S (Arthur Henry Shakespeare), 1853-1936, British Science Guild. South Australian Branch. Handbooks Committee, Perrin, Florence, State Botanical Collection, Royal Botanic Gardens Victoria, Lucas, A H S (Arthur Henry Shakespeare), 1853-1936, British Science Guild. South Australian Branch. Handbooks Committee, and Perrin, Florence
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Marine Algae -- South Australia - Published
- 1936
32. The Seaweeds of South Australia
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Lucas, A H S (Arthur Henry Shakespeare) 1853-1936, British Science Guild. South Australian Branch. Handbooks Committee, Perrin, Florence, State Botanical Collection, Royal Botanic Gardens Victoria, Lucas, A H S (Arthur Henry Shakespeare) 1853-1936, British Science Guild. South Australian Branch. Handbooks Committee, and Perrin, Florence
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Marine Algae -- South Australia - Published
- 1936
33. Adapting the Open Field Test to Assess Anxiety-Related Behavior in Zebrafish
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Godwin, John, primary, Sawyer, Susanna, additional, Perrin, Florence, additional, Oxendine, Sarah E., additional, and Kezios, Zachary D., additional
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- 2012
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34. Inhibiting microglia proliferation after spinal cord injury improves recovery in mice and nonhuman primates.
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Poulen, Gaëtan, Aloy, Emilie, Bringuier, Claire M., Mestre-Francés, Nadine, Artus, Emaëlle V. F., Cardoso, Maïda, Perez, Jean-Christophe, Goze-Bac, Christophe, Boukhaddaoui, Hassan, Lonjon, Nicolas, Gerber, Yannick N., and Perrin, Florence E.
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- 2021
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35. Axonal involvement in the Wlds neuroprotective effect: analysis of pure motoneurons in a mouse model protected from motor neuron disease at a pre-symptomatic age
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Simonin, Yannick, Perrin, Florence E., and Kato, Ann C.
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- 2007
36. Cell death pathways differ in several mouse models with motoneurone disease: analysis of pure motoneurone populations at a presymptomatic age
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Perrin, Florence E., Boisset, Gaelle, Lathuilière, Aurélien, and Kato, Ann C.
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- 2006
37. No widespread induction of cell death genes occurs in pure motoneurons in an amyotrophic lateral sclerosis mouse model
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Perrin, Florence E., Boisset, Gaelle, Docquier, Mylene, Schaad, Olivier, Descombes, Patrick, and Kato, Ann C.
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- 2005
38. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1α and interleukin-1β in Wallerian degeneration
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Perrin, Florence E., Lacroix, Steve, Avilés-Trigueros, Marcelino, and David, Samuel
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- 2005
39. C57BL/6 and Swiss Webster Mice Display Differences in Mobility, Gliosis, Microcavity Formation and Lesion Volume After Severe Spinal Cord Injury
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Noristani, Harun Najib, They, Laetitia, Perrin, Florence Evelyne, Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), and Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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microcavity ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,protection ,mobility ,spinal cord injury ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,ComputingMilieux_MISCELLANEOUS ,Neuroscience ,Original Research ,glial cells - Abstract
International audience; Spinal cord injuries (SCI) are neuropathologies causing enormous physical and emotional anguish as well as irreversibly disabilities with great socio/economic burdens to our society. The availability of multiple mouse strains is important for studying the underlying pathophysiological response after SCI. Although strain differences have been shown to directly affect spontaneous functional recovery following incomplete SCI, its influence after complete lesion of the spinal cord is unclear. To study the influence of mouse strain on recovery after severe SCI, we first carried out behavioral analyses up to 6 weeks following complete transection of the spinal cord in mice with two different genetic backgrounds namely, C57BL/6 and Swiss Webster. Using immunohistochemistry, we then analyzed glial cell reactivity not only at different time-points after injury but also at different distances from the lesion epicenter. Behavioral assessments using CatWalk™ and open field analyses revealed increased mobility (measured using average speed) and differential forelimb gross sensory response in Swiss Webster compared to C57BL/6 mice after complete transection of the spinal cord. Comprehensive histological assessment revealed elevated microglia/macrophage reactivity and a moderate increase in astrogliosis in Swiss Webster that was associated with reduced microcavity formation and reduced lesion volume after spinal cord transection compared to C57BL/6 mice. Our results thus suggest that increased mobility correlates with enhanced gliosis and better tissue protection after complete transection of the spinal cord.
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- 2018
40. Serotonergic mechanisms in spinal cord injury
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Perrin, Florence Evelyne, primary and Noristani, Harun Najib, additional
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- 2019
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41. Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord
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Guillon Hélène, Rigau Valérie, Privat Alain, Lidereau Rosette, Bieche Ivan, Rothhut Bernard, Perrin Florence E, Teigell Marisa, Bony Claire, Ripoll Chantal, Mamaeva Daria, Vachiery-Lahaye Florence, Noel Daniele, Bauchet Luc, and Hugnot Jean-Philippe
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human central nervous system ,spinal cord ,stem cells ,vascular muscle cells ,osteogenesis ,Nkx6.1 ,calcification ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurophysiology and neuropsychology ,QP351-495 - Abstract
Abstract Background The adult central nervous system (CNS) contains different populations of immature cells that could possibly be used to repair brain and spinal cord lesions. The diversity and the properties of these cells in the human adult CNS remain to be fully explored. We previously isolated Nestin+ Sox2+ neural multipotential cells from the adult human spinal cord using the neurosphere method (i.e. non adherent conditions and defined medium). Results Here we report the isolation and long term propagation of another population of Nestin+ cells from this tissue using adherent culture conditions and serum. QPCR and immunofluorescence indicated that these cells had mesenchymal features as evidenced by the expression of Snai2 and Twist1 and lack of expression of neural markers such as Sox2, Olig2 or GFAP. Indeed, these cells expressed markers typical of smooth muscle vascular cells such as Calponin, Caldesmone and Acta2 (Smooth muscle actin). These cells could not differentiate into chondrocytes, adipocytes, neuronal and glial cells, however they readily mineralized when placed in osteogenic conditions. Further characterization allowed us to identify the Nkx6.1 transcription factor as a marker for these cells. Nkx6.1 was expressed in vivo by CNS vascular muscular cells located in the parenchyma and the meninges. Conclusion Smooth muscle cells expressing Nestin and Nkx6.1 is the main cell population derived from culturing human spinal cord cells in adherent conditions with serum. Mineralization of these cells in vitro could represent a valuable model for studying calcifications of CNS vessels which are observed in pathological situations or as part of the normal aging. In addition, long term propagation of these cells will allow the study of their interaction with other CNS cells and their implication in scar formation during spinal cord injury.
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- 2011
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42. Longitudinal Magnetic Resonance Imaging Analysis and Histological Characterization after Spinal Cord Injury in Two Mouse Strains with Different Functional Recovery: Gliosis as a Key Factor
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Noristani, Harun N., primary, Saint-Martin, Guillaume P., additional, Cardoso, Maïda, additional, Sidiboulenouar, Rahima, additional, Catteau, Matthias, additional, Coillot, Christophe, additional, Goze-Bac, Christophe, additional, and Perrin, Florence E., additional
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- 2018
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43. CSF1R Inhibition Reduces Microglia Proliferation, Promotes Tissue Preservation and Improves Motor Recovery After Spinal Cord Injury
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Gerber, Yannick Nicolas, primary, Saint-Martin, Guillaume Patrick, additional, Bringuier, Claire Mathilde, additional, Bartolami, Sylvain, additional, Goze-Bac, Christophe, additional, Noristani, Harun Najib, additional, and Perrin, Florence Evelyne, additional
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- 2018
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44. A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury
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Noristani, Harun n., Boukhaddaoui, Hassan, Saint-martin, Guillaume, Auzer, Pauline, Sidiboulenouar, Rahima, Lonjon, Nicolas, ALIBERT, Eric, Tricaud, Nicolas, GOZE-BAC, Christophe, COILLOT, Christophe, Perrin, Florence e., Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and Aigle, L2c
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[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging ,tissue clearing ,ex vivo diffusion MRI ,nervous system ,microglia/monocytes ,[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Technology Report ,spinal cord injury ,Neuroscience ,two-photon - Abstract
Central nervous system (CNS) injury has been observed to lead to microglia activation and monocytes infiltration at the lesion site. Ex vivo diffusion magnetic resonance imaging (diffusion MRI or DWI) allows detailed examination of CNS tissues, and recent advances in clearing procedures allow detailed imaging of fluorescent-labeled cells at high resolution. No study has yet combined ex vivo diffusion MRI and clearing procedures to establish a possible link between microglia/monocytes response and diffusion coefficient in the context of spinal cord injury (SCI). We carried out ex vivo MRI of the spinal cord at different time-points after spinal cord transection followed by tetrahydrofuran based clearing and examined the density and morphology of microglia/monocytes using two-photon microscopy. Quantitative analysis revealed an early marked increase in microglial/monocytes density that is associated with an increase in the extension of the lesion measured using diffusion MRI. Morphological examination of microglia/monocytes somata at the lesion site revealed a significant increase in their surface area and volume as early as 72 hours post-injury. Time-course analysis showed differential microglial/monocytes response rostral and caudal to the lesion site. Microglia/monocytes showed a decrease in reactivity over time caudal to the lesion site, but an increase was observed rostrally. Direct comparison of microglia/monocytes morphology, obtained through multiphoton, and the longitudinal apparent diffusion coefficient (ADC), measured with diffusion MRI, highlighted that axonal integrity does not correlate with the density of microglia/monocytes or their somata morphology. We emphasize that differential microglial/monocytes reactivity rostral and caudal to the lesion site may thus coincide, at least partially, with reported temporal differences in debris clearance. Our study demonstrates that the combination of ex vivo diffusion MRI and two-photon microscopy may be used to follow structural tissue alteration. Lesion extension coincides with microglia/monocytes density; however, a direct relationship between ADC and microglia/monocytes density and morphology was not observed. We highlighted a differential rostro-caudal microglia/monocytes reactivity that may correspond to a temporal difference in debris clearance and axonal integrity. Thus, potential therapeutic strategies targeting microglia/monocytes after SCI may need to be adjusted not only with the time after injury but also relative to the location to the lesion site.
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- 2017
45. Le tutorat entre pairs dans une école de soins infirmiers
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Hirschy Perrin, Florence
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- 2017
46. RNA-Seq Analysis of Microglia Reveals Time-Dependent Activation of Specific Genetic Programs following Spinal Cord Injury
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Noristani, Harun N., Gerber, Yannick N., Sabourin, Jean-Charles, Le Corre, Marine, Lonjon, Nicolas, Mestre-Frances, Nadine, Hirbec, Hélène E., Perrin, Florence E., Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Neurochirurgie [Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-CHU Gui de Chauliac [Montpellier], Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
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nervous system ,DNA damage ,microglia ,RNA sequencing ,non-human primate ,spinal cord injuries ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,BRCA1 ,ComputingMilieux_MISCELLANEOUS ,Neuroscience ,Original Research - Abstract
International audience; Neurons have inherent competence to regrow following injury, although not spontaneously. Spinal cord injury (SCI) induces a pronounced neuroinflammation driven by resident microglia and infiltrating peripheral macrophages. Microglia are the first reactive glial population after SCI and participate in recruitment of monocyte-derived macrophages to the lesion site. Both positive and negative influence of microglia and macrophages on axonal regeneration had been reported after SCI, raising the issue whether their response depends on time post-lesion or different lesion severity. We analyzed molecular alterations in microglia at several time-points after different SCI severities using RNA-sequencing. We demonstrate that activation of microglia is time-dependent post-injury but is independent of lesion severity. Early transcriptomic response of microglia after SCI involves proliferation and neuroprotection, which is then switched to neuroinflammation at later stages. Moreover, SCI induces an autologous microglial expression of astrocytic markers with over 6% of microglia expressing glial fibrillary acidic protein and vimentin from as early as 72 h post-lesion and up to 6 weeks after injury. We also identified the potential involvement of DNA damage and in particular tumor suppressor genebreast cancer susceptibility gene 1(Brca1) in microglia after SCI. Finally, we established that BRCA1 protein is specifically expressed in non-human primate spinal microglia and is upregulated after SCI. Our data provide the first transcriptomic analysis of microglia at multiple stages after different SCI severities. Injury-induced microglia expression of astrocytic markers at RNA and protein levels demonstrates novel insights into microglia plasticity. Finally, increased microglia expression of BRCA1 in rodents and non-human primate model of SCI, suggests the involvement of oncogenic proteins after CNS lesion.
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- 2017
47. Deleterious effect of Usutu virus on human neural cells
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Constant, Orianne, Desmetz, Caroline, Barthelemy, Jonathan, Lemaitre, Jean-Marc, Milhavet, Ivier, Nagot, Nicolas, Foulongne, Vincent, Perrin, Florence E., Saiz, Juan-Carlos, Lecollinet, Sylvie, Van de Perre, Philippe, Salinas, Sara, and Simonin, Yannick
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west-nile-virus ,japanese encephalitis ,human brain ,infection ,flavivirus ,mice ,induction ,emergence ,tropism ,croatia - Abstract
In the last decade, the number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu virus (USUV) are African mosquitoborne viruses that recently emerged. Recently, ZIKV has been intensely studied due to major outbreaks associated with neonatal death and birth defects, as well as neurological symptoms. USUV pathogenesis remains largely unexplored, despite significant human and veterinary associated disorders. Circulation of USUV in Africa was documented more than 50 years ago, and it emerged in Europe two decades ago, causing massive bird mortality. More recently, USUV has been described to be associated with neurological disorders in humans such as encephalitis and meningoencephalitis, highlighting USUV as a potential health threat. The aim of this study was to evaluate the ability of USUV to infect neuronal cells. Our results indicate that USUV efficiently infects neurons, astrocytes, microglia and IPSc-derived human neuronal stem cells. When compared to ZIKV, USUV led to a higher infection rate, viral production, as well as stronger cell death and anti-viral response. Our results highlight the need to better characterize the physiopathology related to USUV infection in order to anticipate the potential threat of USUV emergence.
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- 2017
48. Use of longitudinal magnetic resonance imaging in preclinical models of spinal cord injury
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Noristani, Harun Najib, Perrin, Florence Evelyne, and Université de Montpellier (UM)
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0303 health sciences ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,[SCCO.NEUR]Cognitive science/Neuroscience ,Magnetic resonance imaging ,medicine.disease ,lcsh:RC346-429 ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Developmental Neuroscience ,Perspective ,medicine ,Radiology ,business ,Spinal cord injury ,lcsh:Neurology. Diseases of the nervous system ,ComputingMilieux_MISCELLANEOUS ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
International audience
- Published
- 2019
49. Brca1 is expressed in human microglia and is dysregulated in human and animal model of ALS
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Noristani, Harun Najib, Sabourin, Jean Charles, Gerber, Yannick Nicolas, Teigell, Marisa, Sommacal, Andreas, dM Vivanco, Maria, Weber, Markus, Perrin, Florence Evelyne, Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Science and Neuroscience Department [Bilbao, Spain], Integrative Biology of Neurodegeneration [Bilbao, Spain], Fundación Ikerbasque [Bilbao]-University of the Basque Country-Fundación Ikerbasque [Bilbao]-University of the Basque Country, Deutsches Forschungszentrum für Künstliche Intelligenz GmbH = German Research Center for Artificial Intelligence (DFKI), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Ikerbasque - Basque Foundation for Science-University of the Basque Country-Ikerbasque - Basque Foundation for Science-University of the Basque Country, École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and Perrin, Florence
- Subjects
hSOD1G93Amice ,DNA-repair ,hSOD1(G93A) mice ,mouse model ,[SCCO.NEUR]Cognitive science/Neuroscience ,[SCCO.NEUR] Cognitive science/Neuroscience ,MOLECULAR BIOLOGY ,Clinical Neurology ,CELLULAR AND MOLECULAR NEUROSCIENCE ,microglia ,amyotophic-lateral-sclerosis ,motor-neuron disease ,gene-expression ,transcriptomics ,ALS patients ,responses ,renal-cell carcinoma ,Microglia ,alzheimers-disease ,protein ,Transcriptomics ,NEUROLOGY ,breast-cancer ,Brca1 - Abstract
Background: There is growing evidence that microglia are key players in the pathological process of amyotrophic lateral sclerosis (ALS). It is suggested that microglia have a dual role in motoneurone degeneration through the release of both neuroprotective and neurotoxic factors. Results: To identify candidate genes that may be involved in ALS pathology we have analysed at early symptomatic age (P90), the molecular signature of microglia from the lumbar region of the spinal cord of hSOD1(G93A) mice, the most widely used animal model of ALS. We first identified unique hSOD1(G93A) microglia transcriptomic profile that, in addition to more classical processes such as chemotaxis and immune response, pointed toward the potential involvement of the tumour suppressor gene breast cancer susceptibility gene 1 (Brca1). Secondly, comparison with our previous data on hSOD1(G93A) motoneurone gene profile substantiated the putative contribution of Brca1 in ALS. Finally, we established that Brca1 protein is specifically expressed in human spinal microglia and is up-regulated in ALS patients. Conclusions: Overall, our data provide new insights into the pathogenic concept of a non-cell-autonomous disease and the involvement of microglia in ALS. Importantly, the identification of Brca1 as a novel microglial marker and as possible contributor in both human and animal model of ALS may represent a valid therapeutic target. Moreover, our data points toward novel research strategies such as investigating the role of oncogenic proteins in neurodegenerative diseases. We are grateful to ALS patients and their relatives that donate their tissues. We acknowledge the New York Brain Bank-The Taub Institute, Columbia University (NYBB). The hybridoma CD11b antibody developed by Timothy A. Springer was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology, Iowa city, IA 52242. We thank the iGE3Genomics Platform, University of Geneva Switzerland for their assistance in transcriptomic and qPCR analysis. This work was supported by the Spanish Government, Plan Nacional de I+D+I 2008-2011 and ISCIII-Subdireccion General de Evaluacion y Fomento de la investigacion (PI10/00709) [to FEP], the Government of the Basque Country grant (Proyectos de Investigacion Sanitaria and Fondo Comun de Cooperacion Aquitania-Euskadi) [to FEP], the "Fondation pour la Recherche Medicale" [to FEP] and the French Government, ANR-FNS grant, GliALS (No ANR-14-CE36-0009-01) [to FEP], the patient organisations "Demain Debout Aquitaine" [to YNG and HNN] and "Verticale" [to FEP and HNN].
- Published
- 2015
50. A Novel Translational Model of Spinal Cord Injury in Nonhuman Primate
- Author
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Le Corre, Marine, primary, Noristani, Harun N., additional, Mestre-Frances, Nadine, additional, Saint-Martin, Guillaume P., additional, Coillot, Christophe, additional, Goze-Bac, Christophe, additional, Lonjon, Nicolas, additional, and Perrin, Florence E., additional
- Published
- 2017
- Full Text
- View/download PDF
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