Search

The current study examined the characteristics of physiological synchrony between grandmothers and grandchildren in Chinese three-generation families, and the associations between physiological synchrony and child emotion regulation. The participants included 92 children (age 8-10-year old) and their grandmothers. Respiratory sinus arrhythmia (RSA) was collected from both grandmothers and their grandchildren throughout a collaborative drawing task and a conflict discussion task. Child emotion regulation was measured using the Children's Emotional Management Scale. We found no evidence for an overall pattern of concordant or discordant synchrony within dyads. Instead, there was great variability in patterns of synchrony across dyads. During the collaborative drawing task, concordance in grandmother's RSA and grandchildren's subsequent RSA was linked with better emotion regulation. During the conflict discussion, concordance in grandmother's RSA and grandchildren's simultaneous RSA was linked with poorer emotion regulation. These results suggest that grandmother-grandchild synchrony in different directions, time lags, and contexts has different influences on children's emotion regulation. The findings of this study highlight the importance of contextual physiological co-regulation between Chinese children and their grandmothers for children's social-emotional development., (© 2024 Wiley Periodicals LLC.)

Longitudinal multimethod data across three time points were examined to explore the associations between previously institutionalized toddlers' ( N = 71; 59% female) socioemotional skills (Time Point 1: 18 months to 3-years-old), executive functioning (i.e., attention, working memory, inhibitory control) in the preschool years (Time Point 2: 2-4-years-old), and adjustment in kindergarten (5-6-years-old). Children were from multiple regions (35% Eastern European, 31% Southeast Asian, 25% African, and 9% Latin American), and 90% of adoptive parents were White from the Midwestern United States. Socioemotional competency at Time Point 1 was associated with fewer attention problems and greater inhibitory control at Time Point 2, which were subsequently associated with more observed social competence, greater observed classroom competence, and less teacher-reported teacher-child conflict in kindergarten. Indirect effects from socioemotional competencies in toddlerhood to kindergarten adjustment via executive functioning during the preschool period emerged. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Using a multimethod, multiinformant longitudinal design, we examined associations between specific forms of positive and negative emotional reactivity at age 5, children's effortful control (EC), emotion regulation, and social skills at age 7, and adolescent functioning across psychological, academic, and physical health domains at ages 15/16 ( N = 383). We examined how distinct components of childhood emotional reactivity directly and indirectly predict domain-specific forms of adolescent adjustment, thereby identifying developmental pathways between specific types of emotional reactivity and adjustment above and beyond the propensity to express other forms of emotional reactivity. Age 5 high-intensity positivity was associated with lower age 7 EC and more adolescent risk-taking; age 5 low-intensity positivity was associated with better age 7 EC and adolescent cardiovascular health, providing evidence for the heterogeneity of positive emotional reactivity. Indirect effects indicated that children's age 7 social skills partially explain several associations between age 5 fear and anger reactivity and adolescent adjustment. Moreover, age 5 anger reactivity, low-, and high-intensity positivity were associated with adolescent adjustment via age 7 EC. The findings from this interdisciplinary, long-term longitudinal study have significant implications for prevention and intervention work aiming to understand the role of emotional reactivity in the etiology of adjustment and psychopathology.

The present study examined the influence of fathers' parenting quality during infancy on children's emotion regulation during toddlerhood and, subsequently, attention-deficit/hyperactivity disorder (ADHD) symptoms in middle childhood. Fathers and their 8-month-old infants ( N = 124) were followed over time to obtain home observations of parenting quality at 8 and 24 months, laboratory observations of children's emotion regulation at 24 months, and teacher reports of children's ADHD symptoms at 7 years. A path analysis revealed that fathers' emotional disengagement in infancy and minimizing responses to children's distress in toddlerhood forecast children's development of ADHD symptoms in middle childhood. Further, a significant indirect effect was found such that fathers' parenting at 8 and 24 months predicted subsequent development of ADHD symptoms at age 7 through toddlers' difficulty regulating emotion. Implications of this study for early intervention and directions for future research are discussed.

Longitudinal trajectories of psychopathology in previously institutionalized (PI) youth were identified and biobehavioral emotion regulation processes were examined as developmental mechanisms that predict these trajectories. Mental health data were collected from PI ( N = 132) and nonadopted (NA; N = 175) youth across four time points (participant age ranged from 7- to 21-year-old). Using semiparametric group-based methods, the probability that each individual belonged to a distinct group that followed a specific pattern of behavior across time was estimated. We then tested whether unique aspects of emotion regulation (global, observed, and biological) were differentially associated with membership in externalizing and internalizing trajectory groups using multinomial logistic regression models. Four externalizing trajectories were identified for the PI and NA groups. For PI youth, global, observed, and biological emotion regulation processes were uniquely predictive of more adaptive externalizing trajectories. For NA youth, only parent-reported global emotion regulation was predictive of externalizing patterns. Three internalizing trajectories were identified for PI and NA youth. Generally, only parent-reported global emotion regulation predicted internalizing group membership for both PI and NA youth. Results suggest that biobehavioral emotion regulation processes may be particularly important predictors and potential points of intervention when targeting trajectories of externalizing behaviors in PI children.

Flavonoids acylated on their core phenolic groups are rare. The Aotearoa New Zealand endemic alpine daisy Celmisia viscosa is widespread, but its flavonoids have not previously been identified. Leaf extracts yielded a series of 8- O -acylated flavones with combinations of 3-methylbutanoate, 2-methylbutanoate, and 2-methylpropanoate groups and one, two, or three O -methyls, all previously unreported. Regiochemistries of 8-(3″-methylbutanoyl)-5-hydroxy-6,7,4'-trimethoxyflavone ( 5 ) and 8-(2″-methylbutanoyl)-5,7,4'-trihydroxy-6-methoxyflavone ( 10 ) were defined by X-ray crystallography. LC analyses of leaf extracts from the full geographic range of C. viscosa showed intraspecific variation of these flavones: most had high concentrations of trimethoxy 8- O -acylated flavones, but dimethoxy 8- O -acylated flavones were the most abundant flavonoids in two individuals. Three other viscid (sticky leaved) Celmisa species also contained these rare flavones, but four nonviscid Celmisa had none detectable.

The methyl-migrated bicyclic skeleton of the halimane diterpenes has been found in a wide range of organisms, including flowering plants, liverworts, marine animals, and bacteria. The discovery of halima-1(10),14-dien-13-ol ( 3 ) from the Aotearoa New Zealand endemic alpine daisy Celmisia viscosa is now reported. The full configuration was assigned for the first time by X-ray crystallography, enantiomeric to that of a liverwort isolate. The absolute configuration at C-5 of the halimane is opposite to that at C-5 of the labdane epimanool ( 1 ) found in some C. viscosa specimens. Two new 2,6-dideoxyhexopyran-3-uloside halimane derivatives ( 4 and 5 ) were also found, and the absolute configuration of 5 was determined by 1 H NMR analysis of the Mosher esters. Line broadening in the 13 C NMR spectra of these halim-1(10)-enes was due to conformational exchange in the decalin ring A, as shown by molecular modeling and DFT calculations. 1 H NMR and GC analyses of leaf extracts of individual plants from across the full geographic range of C. viscosa revealed intraspecific variation of diterpenes: 37 samples had halimadienol as the main diterpene in large amounts and 2 specimens had predominantly epimanool, again in large amounts. Three other viscid (sticky leaved) Celmisia species also contained diterpenes, but none was detectable in four nonviscid Celmisia species.

Introduction: Hydrogen is the most efficient and economical carrier gas for gas chromatography (GC). However, there are rare reports of artefact formation by hydrogenation of unsaturated compounds on GC. Head space solid-phase microextraction (HS-SPME) GC conditions for hydrogenation were studied., Methodology: HS-SPME-GC-mass spectrometry (MS) analyses of common classes of plant volatiles were carried out using hydrogen (H 2 ) and helium (He) carrier gases with different SPME fibre coatings, GC inlet temperatures, and desorption times., Results: Common phenylpropanoids, monoterpenes, and green leaf volatiles were hydrogenated to varying degrees on HS-SPME-GC with H 2 carrier gas and SPME fibres coated with polydimethylsiloxane (PDMS)/Carboxen (CAR), PDMS/divinylbenzene (DVB), and PDMS/CAR/DVB. No artefacts were detected using PDMS-only coated fibres or He carrier gas., Conclusion: Unsaturated plant volatiles may be hydrogenated on HS-SPME-GC when using H 2 carrier gas with SPME fibre coatings containing DVB polymer or CAR porous particles. Parallel analyses with He and H 2 carrier gases are recommended when developing HS-SPME-GC methods for plant volatiles, or use of PDMS-only coated fibres., (© 2021 John Wiley & Sons, Ltd.)

Kunzea (Myrtaceae) trees and shrubs, generally called kānuka, grow across most of Aotearoa/New Zealand (NZ). With the exception of K. sinclairii, an offshore island endemic, kānuka had been treated as an Australasian species K. ericoides. However, a 2014 taxonomic revision recognized ten species, all endemic to NZ. Kānuka chemistry is less studied than that of its closest relative in NZ, mānuka (Leptospermum scoparium), which shows very distinct regional foliage chemotypes. We have used a miniaturized method with GC and 1 H NMR to analyze foliage chemistry of voucher specimens from across the geographic ranges of the ten NZ Kunzea species. We found common mono- and sesquiterpenes, with α-pinene dominant in all samples, but only traces of antimicrobial triketones. Two unusual flavanones, with unsubstituted B-rings and known bioactivity against Phytophthora, did distinguish some of the samples. 5,7-Dihydroxy-6,8-dimethyl flavanone was only found at high concentrations in the three K. sinclairii samples in this study's sample set, but this compound has separately been reported in K. robusta samples from a nearby region. Therefore none of the NZ Kunzea species was distinguished by the chemistry analyzed in this study, but there is a possibility of regional flavonoid chemotypes cutting across the species boundaries., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The current study investigated the association between cortisol stress reactivity to a social stressor and observed socially anxious behaviors both concurrently and over time among previously institutionalized (PI) (N = 132; ages 7-17) youth and a comparison non-adopted (NA) sample (N = 176). Cortisol reactivity was captured during the Trier Social Stress Test for Children (TSST-C; Yim et al., 2015) and youths' social anxiety behaviors were coded during the speech portion of the TSST-C. Autoregressive cross-lagged panel models with structured residuals showed that for PI youth, greater cortisol reactivity predicted increases in socially anxious behavior during the TSST-C across three sessions. However, greater cortisol reactivity was negatively associated with concurrent social anxiety behavior. Thus, increases in cortisol reactivity across adolescence may aid in behavioral control in social situations in the short-term but may exacerbate PI youths' socially anxious behavior over time. No significant associations emerged for NA youth., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The current study assessed the associations between pandemic-related stressors and physiological stress, as indexed by hair cortisol concentration (HCC), for mothers and their children (N = 180) aged 5-14-years old (M = 8.91). The associations between maternal HCC and children's HCC and children's behavioral adjustment were also examined. Mothers reported on COVID-19-related behaviors and children's adjustment, and both mother and child participants collected and mailed hair samples between August and November of 2020. Results indicated that higher maternal HCC was correlated with living in a more urban environment, job loss, working from home, exposure to pandemic-related news, and social isolation. Child HCC was correlated with family job loss and social isolation. Mother HCC and child HCC were significantly associated, and this association was moderated by child age; younger children's HCC was more strongly associated with mothers' HCC than older children's HCC. Finally, maternal HCC was associated with greater child internalizing symptoms, but was not associated with children's externalizing symptoms. Child HCC was not associated with child behavior., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The current study investigated whether recalibration of the hypothalamic-pituitary -adrenocortical (HPA) axis stress response in youth who had previously experienced early life stress (ELS) would predict socioemotional adjustment in a follow-up assessment approximately 2-4 years later when youth were 12- to 21-years old. The sample consisted of previously institutionalized (PI) (N = 96) youth and a comparison non-adopted (NA) group (N = 117). Youth were 16 years old on average at the time of the follow-up assessment. Parent and youth-reported measures were used to assess youth's internalizing symptoms and emotion regulation. Parent-reported measures were used to assess youth's externalizing symptoms. We tested whether showing cortisol increases (vs. not) across the peripubertal period was associated with later social and emotional adjustment differently for PI and NA youth. Significant interactions emerged showing that for PI youth only, increases in cortisol reactivity across the peripubertal period was associated with poorer subsequent socioemotional functioning., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Objective: Sleep is vital for healthy development, yet most adolescents do not meet recommended nightly hours. Although racial/ethnic minorities often experience relatively worse sleep outcomes compared with White peers, little is known about how the sleep-mental health relationship holds across diverse groups or how family relationships affect this association., Method: Using data on 8th, 9th, and 11th grade public school respondents to the 2016 Minnesota Student Survey (N = 113,834), we conducted univariate, bivariate, and multivariate analyses to examine whether sleep duration was associated with depressive symptoms, suicide ideation, and suicide attempt, adjusting for sociodemographic covariates. Furthermore, we examined the effect of the parent-child connectedness by sleep interaction on these relationships. Analyses were conducted for 9 racial/ethnic groups collectively and separately., Results: Overall, youth sleep duration and parent-child connectedness were independently associated with reduced rates of depressive symptoms, suicide ideation, and suicide attempt. There was significant interaction between parent-child connectedness and sleep, demonstrating that connectedness magnifies the benefits of the sleep-mental health relationship. Main effects of sleep and parent-child connectedness for mental health were similar for most individual racial/ethnic groups, although magnitudes varied. The connectedness-sleep interaction only remained significant for White and Asian youth on select suicide-related outcomes., Conclusion: Despite racial/ethnic differences, adolescent sleep and parent-child connectedness both seem to buffer youth from poor mental health in a large, multiethnic sample. On the whole, these factors demonstrate a synergistic protective effect and reflect promising intervention targets. The extent to which their interactive benefit translates across diverse populations requires additional study., Competing Interests: Disclosure: The authors declare no conflict of interest., (Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.)

The Early Emotion Regulation Behavior Questionnaire (EERBQ) assesses children's emotion regulation (ER) behavioral strategies in both positive and negative emotional contexts. Psychometric properties and factor structure were tested in a sample of caregivers across the United States ( N = 362) with children ages 2-6 years-old (56% male; 73% White). Findings suggest that the EERBQ is psychometrically sound and correlates with other well-established measures of children's socioemotional functioning. Previously, researchers have only been able to assess children's emotional behavioral regulatory strategies in a laboratory setting. Thus, use of the EERBQ addresses a critical gap in the current literature by providing researchers and practitioners with an instrument to measure young children's early emotional functioning outside of a laboratory context. This is particularly salient because early difficulty regulating emotions is often a precursor to persistent adverse developmental outcomes. Thus, the ability to easily to collect rich and predictive behavioral regulation data is imperative for early identification and treatment of youths' emotional and behavioral problems.

2-O-β-d-Glucopyranosyl l-ascorbic acid (AA-2βG) is a stable, bioavailable vitamin C (AA) derivative. We report the distribution and seasonal variation of AA-2βG in apples and its occurrence in other domesticated crops and in wild harvested Ma̅ori foods. Liquid chromatography-mass spectrometry analyses showed high AA-2βG concentrations in crab apples ( Malus sylvestris ) but low concentrations in domesticated apples. Leaves of crab and domesticated apple cultivars contained similar intermediate AA-2βG concentrations. Fruits and leaves of other crops were analyzed: mainly Rosaceae but also Actinidiaceae and Ericaceae. AA-2βG was detected in all leaves (0.5-6.1 mg/100 g fr. wt.) but was at lower concentrations in most fruits (0.0-0.5 mg/100 g fr. wt.) except for crab apples (79.4 mg/100 g fr. wt.). Ma̅ori foods from Solanaceae, Piperaceae, Asteraceae, and a fern of Aspleniaceae also contained AA-2βG. This extensive occurrence suggests a general role in AA metabolism for AA-2βG.

In this study (N=160), we observed children's private (i.e., self-directed) speech (PS) during a challenging puzzle task at age 3 and assessed whether the amount and maturity of their PS predicted their inhibitory control (IC) at age 4 and indirectly emotion regulation at age 9. Additionally, we examined whether the direct and indirect effects of PS were moderated by children's temperament. As expected, the maturity of children's PS was positively associated with IC and this association was stronger when children were reported as higher in anger reactivity by mothers (the interaction accounting for 11% of the explained variance). Children low in temperamental anger tend to have good IC and may not need to use PS. When children were at or above the mean on anger reactivity, PS maturity was indirectly associated with better emotion regulation at age 9 through an influence on IC at age 4 (index of moderated mediation =1.03 [.10, 3.60]). Findings suggest that PS is an important self-regulatory tool for 3-year-olds who typically experience and express anger.

Megan Gunnar's pubertal stress recalibration hypothesis was supported in a recent study of previously institutionalized (PI) youth such that increases in pubertal stage were associated with increases in cortisol stress reactivity. This work provides evidence that puberty may open up a window of recalibration for PI youth, resulting in a shift from a blunted to a more typical cortisol stress response. Using the same sample (N = 132), the current study aimed to elucidate whether increases in cortisol are associated with increases in adaptive functioning or whether they further underlie potential links to developmental psychopathology. Specifically, we examined the bidirectional associations between cortisol stress reactivity and both internalizing and externalizing symptoms across three timepoints during the pubertal period. Youth reported on their own internalizing symptoms and parents reported on youths' externalizing symptoms. Cortisol reactivity was assessed during the Trier social stress test. Analyses revealed no associations between cortisol reactivity and externalizing symptoms across puberty for PI youth. However, longitudinal bidirectional associations did emerge for internalizing symptoms such that increases in cortisol reactivity predicted increases in internalizing symptoms and increases in internalizing symptoms predicted increases in cortisol reactivity. Findings suggest that recalibrating to more normative levels of cortisol reactivity may not always be associated with adaptive outcomes for PI youth.

Maturation of the prefrontal cortex (PFC) across the first few years of life is thought to underlie the emergence of inhibitory control (IC) abilities, which may play an important role in children's early academic success. In this growth curve modeling study (N = 364), we assessed developmental change in children's resting-state electroencephalogram (EEG) activity (6-9 Hz 'alpha' power) from 10 months to 4 years and examined whether the initial levels or amount of change in frontal alpha power were associated with children's IC at age 4 and indirectly academic skills at age 6. Results indicated that greater increases in frontal alpha power across the study period were associated with better IC, and indirectly with better performance on Woodcock-Johnson tests of reading and math achievement at age 6. Similar associations between change in EEG and age 4 vocabulary were observed but did not mediate an association with academic skills. Similar analyses with posterior alpha power showed no associations with IC. Findings underscore the significance of frontal lobe maturation from infancy to early childhood for children's intellectual development., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Non-targeted LC-MS metabolomics on fruit of three wild and domesticated apple species (Malus sylvestris, M. sieversii and M. domestica) showed that two crab apple (M. sylvestris) accessions were distinguished by high concentrations of an ascorbic acid glycoside (AAG). This was partly purified, but key NMR signals were masked by inseparable sucrose. Reference samples of 2-O-β-D-glucopyranosyl L-ascorbic acid and 2-O-β-D-galactopyranosyl L-ascorbic acid were synthesised, but both coincided with the crab apple AAG on LC-MS. Peracetylation of the crab apple extract allowed both purification and characterisation, and the AAG was proven to be 2-O-β-D-glucopyranosyl L-ascorbic acid by comparison of 1 H NMR, HRMS and HPLC data with synthesised peracetylated ascorbyl glycoside standards. The stability of the natural AA 2-β-glycoside was similar to synthetic 2-O-α-D-glucopyranosyl L-ascorbic acid, used widely in cosmetic and pharmaceutical products. This discovery in crab apples (Rosaceae) is only the fourth reported occurrence of any ascorbyl glycoside from plants, the others being from Cucurbitaceae, Solanaceae and Brassicaceae. It is hypothesised that AAGs may be more widespread in plants than currently realised., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Parasympathetic nervous system functioning as indexed by respiratory sinus arrhythmia (RSA) is widely used as a measure of physiological regulation. We examined developmental patterns of children's resting RSA and RSA reactivity from 2 to 15 years of age, a period of time that is marked by considerable advances in children's regulatory abilities. Physiological data were collected from a community sample of 270 children (116 males) during a resting period and during a frustration laboratory task when the children were 2, 4, 5, 7, 10, and 15 years old. We examined both stability and continuity in resting RSA and RSA reactivity across time. We found stability in resting RSA but not RSA reactivity from toddlerhood to adolescence. Separate multilevel models were used to examine changes in resting RSA and RSA reactivity from Age 2 to Age 15. The rate of change in resting RSA slowed from Age 2 to Age 15 with a plateau around Age 10. A splined growth model indicated that the rate of RSA reactivity increased from Age 2 to Age 7 and a modest slowing and leveling off from Age 7 to Age 15. Understanding the developmental characteristics of RSA across childhood and adolescence is important to understanding the larger constructs of self- and emotion regulation. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are commercially important omega-3 fatty acids found in fish oils. Here we demonstrate that a handheld Raman spectrometer can be used to quantitate these compounds in intact fish oil capsules, avoiding oxidizing risk. Partial least squares regression models were prepared by relating Raman spectral variance to EPA and DHA concentrations determined using gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters in 15 commercial samples containing 145-473 mg g -1 EPA and 101-260 mg·g -1 DHA. Handheld Fourier transform (FT)-Raman models had root mean square errors of cross-validation of 38 mg g -1 , 24 mg g -1 , and 32 mg·g -1 for EPA, DHA, and EPA+DHA, respectively. Models generated from a benchtop FT-Raman spectrometer had corresponding errors of 32 mg·g -1 , 22 mg·g -1 , and 26 mg·g -1 . By comparison, average standard deviations from triplicate GC-MS analyses were 11 mg·g -1 for EPA and 9 mg·g -1 for DHA.

A fundamental question in developmental science is how parental emotion socialization processes are associated with children's subsequent adaptation. Few extant studies have examined this question across multiple developmental periods and levels of analysis. Here, we tested whether mothers' supportive and nonsupportive reactions to their 5-year-old children's negative emotions were associated with teacher and adolescent self-reported adjustment at age 15 via children's physiological and behavioral emotion regulation at age 10 (N = 404). Results showed that maternal supportive reactions to their children's negative emotions were associated with children's greater emotion regulation in a laboratory task and also a composite of mother and teacher reports of emotion regulation at age 10. Maternal nonsupportive reactions to their children's negative emotions were uncorrelated with supportive reactions, but were associated with poorer child physiological regulation and also poorer mother- and teacher-reported emotion regulation at age 10. In turn, better physiological regulation at age 10 was associated with more adolescent-reported social competence at age 15. Furthermore, teacher and mother reports of emotion regulation at age 10 were associated with increased adolescent adjustment across all domains. Mediational effects from nonsupportive and supportive reactions to adolescent adjustment tested via bootstrapping were significant. Our findings suggest that mothers' reactions to their children's negative emotions in early childhood may play a role in their children's ability to regulate their arousal both physiologically and behaviorally in middle childhood, which in turn may play a role in their ability to manage their emotions and behaviors and to navigate increasingly complex social contexts in adolescence. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

This study examined cognitive-affective strategies as predictors of hypothalamic-pituitary-adrenal (HPA) axis responses to a social-evaluative stressor in adolescence as compared to late childhood as a function of early life experiences. Participants included 159 children (9-10 years) and adolescents (15-16 years) divided into two groups based on early care experiences: non-adopted youth raised in their birth families (n = 81) and post-institutionalized youth internationally adopted from orphanage care (n = 78). Youth completed a version of the Trier Social Stress Test modified for use with children and reported on their trait emotion regulation and coping strategies. Children reported more use of suppression and disengagement than adolescents, while adolescents reported more engagement coping strategies. Non-adopted and post-institutionalized youth did not differ in reported strategies. Cognitive reappraisal predicted higher cortisol reactivity in non-adopted children and adolescents, and was not associated with reactivity in the post-institutionalized group. This study has implications for efforts aimed at promoting self-regulation and adaptive stress responses during the transition to adolescence for both typically developing children and children who experienced adverse early care., (© 2019 Wiley Periodicals, Inc.)

The current study compared behavioral and adrenocortical functioning of maltreated ( N = 91) and comparably aged (1.5-3 years) institutionally-reared children soon after (1.5-2.5 months) placement in foster care or adoptive homes, respectively. Foster and adoptive parents reported on the children's socioemotional competence and behavior problems, experimenters scored fear and positive affect to laboratory tasks, and diurnal cortisol measures were obtained. We sought to address whether these early contexts, characterized by different types of neglect and deprivation, have differential effects on young children's development. We found little support for the argument that either context results in uniformly poorer functioning soon after removal from adversity. Maltreated children were less fearful and more positive when exposed to both positive and novel events than institutionally-reared children. However, maltreated children were reported to have more behavior problems than did institutionally-reared children, and institutionally-reared children showed more typical declines in cortisol throughout the day than the maltreated children. These findings increase our ability to construct more targeted and effective interventions for these populations.

Strigolactones (SLs) are multifunctional plant hormones regulating essential physiological processes affecting growth and development. In vascular plants, SLs are recognized by α/β hydrolase-fold proteins from the D14/DAD2 (Dwarf14/Decreased Apical Dominance 2) family in the initial step of the signaling pathway. We have previously discovered that N -phenylanthranilic acid derivatives (e.g. tolfenamic acid) are potent antagonists of SL receptors, prompting us to design quinazolinone and quinazolinedione derivatives (QADs and QADDs, respectively) as second-generation antagonists. Initial in silico docking studies suggested that these compounds would bind to DAD2, the petunia SL receptor, with higher affinity than the first-generation compounds. However, only one of the QADs/QADDs tested in in vitro assays acted as a competitive antagonist of SL receptors, with reduced affinity and potency compared with its N -phenylanthranilic acid 'parent'. X-ray crystal structure analysis revealed that the binding mode of the active QADD inside DAD2's cavity was not that predicted in silico , highlighting a novel inhibition mechanism for SL receptors. Despite a ∼10-fold difference in potency in vitro , the QADD and tolfenamic acid had comparable activity in planta , suggesting that the QADD compensates for lower potency with increased bioavailability. Altogether, our results establish this QADD as a novel lead compound towards the development of potent and bioavailable antagonists of SL receptors., (© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)

In the current study, we compared emotion regulation abilities between post-institutionalized (PI; N = 124) and never-institutionalized non-adopted (NA; N = 172) children and adolescents (7-15 years). We assessed cortisol reactivity and coded emotion regulation during the speech portion of Trier Social Stress Test (TSST-M). Parents reported on their children's social, academic, and behavioral adjustment. Results suggest that emotion regulation abilities increased with age, but this increase was greater for NA than PI youth. With regard to cortisol, piecewise growth modeling revealed that at higher levels of emotion regulation PI youth had greater baseline values (after a period of time allowing for acclimation to the laboratory) and had steeper recovery slopes than NA youth. There was also a main effect of emotion regulation on the reactivity slope suggesting that for both groups, as emotion regulation increased, the cortisol reactivity slope decreased. Finally, greater emotion regulation predicted fewer internalizing behavior problems for PI youth but not for NA youth., (© 2019 Wiley Periodicals, Inc.)

An empirical model of temperament that assessed transactional and cascade associations between respiratory sinus arrhythmia (RSA), negative affectivity, and the caregiving environment (i.e., maternal intrusiveness) across three time points during infancy (N = 388) was examined. Negative affectivity at 5 months was associated positively with maternal intrusiveness at 10 months, which in turn predicted increased negative affectivity at 24 months. RSA at 5 months was associated positively with negative affectivity at 10 months, which subsequently predicted greater RSA at 24 months. Finally, greater RSA at 5 months predicted greater negative affectivity at 10 months, which in turn predicted greater maternal intrusiveness at 24 months. Results are discussed from a biopsychosocial perspective of development., (© 2017 The Authors. Child Development © 2017 Society for Research in Child Development, Inc.)

We examined longitudinal associations across an 8-year time span between overcontrolling parenting during toddlerhood, self-regulation during early childhood, and social, emotional, and academic adjustment in preadolescence (N = 422). Overcontrolling parenting, emotion regulation (ER), and inhibitory control (IC) were observed in the laboratory; preadolescent adjustment was teacher-reported and child self-reported. Results from path analysis indicated that overcontrolling parenting at age 2 was associated negatively with ER and IC at age 5, which, in turn, were associated with more child-reported emotional and school problems, fewer teacher-reported social skills, and less teacher-reported academic productivity at age 10. These effects held even when controlling for prior levels of adjustment at age 5, suggesting that ER and IC in early childhood may be associated with increases and decreases in social, emotional, and academic functioning from childhood to preadolescence. Finally, indirect effects from overcontrolling parenting at age 2 to preadolescent outcomes at age 10 were significant, both through IC and ER at age 5. These results support the notion that parenting during toddlerhood is associated with child adjustment into adolescence through its relation with early developing self-regulatory skills. (PsycINFO Database Record, ((c) 2018 APA, all rights reserved).)

Parasympathetic nervous system functioning in infancy may serve a foundational role in the development of cognitive and socioemotional skills (Calkins, 2007). In this study (N = 297), we investigated the potential indirect effects of cardiac vagal regulation in infancy on children's executive functioning and social competence in preschool via expressive and receptive language in toddlerhood. Vagal regulation was assessed at 10 months during two attention conditions (social, nonsocial) via task-related changes in respiratory sinus arrhythmia (RSA). A path analysis revealed that decreased RSA from baseline in the nonsocial condition and increased RSA in the social condition were related to larger vocabularies in toddlerhood. Additionally, children's vocabulary sizes were positively related to their executive function and social competence in preschool. Indirect effects from vagal regulation in both contexts to both 4-year outcomes were significant, suggesting that early advances in language may represent a mechanism through which biological functioning in infancy impacts social and cognitive functioning in childhood., (© 2018 Wiley Periodicals, Inc.)

We examined associations between specific self-regulatory mechanisms and externalizing behavior patterns from ages 2 to 15 (N = 443). The relation between multiple self-regulatory indicators across multiple domains (i.e., physiological, attentional, emotional, and behavioral) at age 2 and at age 5 and group membership in four distinct externalizing trajectories was examined. By examining each of these self-regulatory processes in combination with one another, and therefore accounting for their shared variance, we aimed to better understand which specific self-regulatory skills were associated most strongly with externalizing behavioral patterns. Findings suggest that behavioral inhibitory control and emotion regulation are particularly important in distinguishing between children who show normative declines in externalizing behaviors across early childhood and those who demonstrate high levels through adolescence.

Some honeys contain the neurotoxin tutin (1) plus hyenanchin (2), 2-(β-d-glucopyranosyl)tutin (3), and 2-[6'-(α-d-glucopyranosyl)-β-d-glucopyranosyl]tutin (4). These honeys are made by bees collecting honeydew from passionvine hoppers feeding on the sap of tutu plants ( Coriaria spp.). We report a LC-MS study showing that all these picrotoxanes are of plant, not insect, origin. Hyenanchin was barely detectable and the diglucoside was not detectable in C. arborea leaves, but tutu phloem sap contained all four compounds at concentrations up to the highest found in honeydew. It is proposed that the diglucoside may function as a transport form of tutin, analogous to sucrose transport in phloem.

The strigolactone (SL) family of plant hormones regulates a broad range of physiological processes affecting plant growth and development and also plays essential roles in controlling interactions with parasitic weeds and symbiotic fungi. Recent progress elucidating details of SL biosynthesis, signaling, and transport offers many opportunities for discovering new plant-growth regulators via chemical interference. Here, using high-throughput screening and downstream biochemical assays, we identified N -phenylanthranilic acid derivatives as potent inhibitors of the SL receptors from petunia (DAD2), rice (OsD14), and Arabidopsis (AtD14). Crystal structures of DAD2 and OsD14 in complex with inhibitors further provided detailed insights into the inhibition mechanism, and in silico modeling of 19 other plant strigolactone receptors suggested that these compounds are active across a large range of plant species. Altogether, these results provide chemical tools for investigating SL signaling and further define a framework for structure-based approaches to design and validate optimized inhibitors of SL receptors for specific plant targets., (© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.)

The New Zealand glowworm, Arachnocampa luminosa, is well-known for displays of blue-green bioluminescence, but details of its bioluminescent chemistry have been elusive. The glowworm is evolutionarily distant from other bioluminescent creatures studied in detail, including the firefly. We have isolated and characterised the molecular components of the glowworm luciferase-luciferin system using chromatography, mass spectrometry and 1 H NMR spectroscopy. The purified luciferase enzyme is in the same protein family as firefly luciferase (31% sequence identity). However, the luciferin substrate of this enzyme is produced from xanthurenic acid and tyrosine, and is entirely different to that of the firefly and known luciferins of other glowing creatures. A candidate luciferin structure is proposed, which needs to be confirmed by chemical synthesis and bioluminescence assays. These findings show that luciferases can evolve independently from the same family of enzymes to produce light using structurally different luciferins.

Research Findings: This study examines whether the development of social skills during childhood serves as a mechanism through which temperamental anger and positive reactivity in toddlerhood influences children's academic competence during preadolescence (N = 406). Temperamental anger at age 2 was negatively associated with children's social skills at age 7; in turn, children's social skills at age 7 were negatively associated with teacher report of academic competence and child and teacher report of school problems at age 10. All three indirect effects were significant suggesting that children's social skills at age 7 is one mechanism through which temperamental anger at age 2 is associated with age 10 child- and teacher-reported school problems. Temperamental positive reactivity was not associated with children's social skills or academic competence., Practice or Policy: Results provide support for early entry points to teach toddlers, especially those high in anger reactivity, the skills to engage in socially appropriate interactions with classmates and teachers, which may lessen subsequent academic challenges.

Fish oils are the primary dietary source of ω-3 polyunsaturated fatty acids (PUFA), but these compounds are prone to oxidation, and commercial fish oil supplements sometimes contain less PUFA than claimed. These supplements are predominantly sold in softgel capsules. In this work, we show that Fourier transform (FT)-Raman spectra of fish oils (n = 5) and ω-3 PUFA concentrates (n = 6) can be acquired directly through intact softgel (gelatin) capsules. These spectra could be used to rapidly distinguish supplements containing ethyl esters from those containing triacylglyceride oils. Raman spectroscopy calibrated with partial least-squares regression against traditional fatty acid methyl ester analyses by gas chromatography-mass spectrometry could be used to rapidly and nondestructively quantitate PUFA and other fatty acid classes directly though capsules. We also show that FT-Raman spectroscopy can noninvasively detect oxidation with high sensitivity. Oils with peroxide values of as low as 10 mequiv kg -1 , which are on the cusp of falling outside of specification, could be readily distinguished from oils that were within specification (7 mequiv kg -1 ).

The genera Bulbine, Bulbinella and Kniphofia produce phenylanthraquinones and are mostly found in southern Africa, although a disjunct group of Bulbinella species endemic to New Zealand also contain phenylanthraquinones as reported herein. The sub-Antarctic megaherb B. rossii yielded sulphated phenylanthraquinones, including a phenylanthraquinone found to carry a sulphated glycoside substituent, 4'-O-demethylknipholone-4'-β-D-xylopyranosyl-3″-sulphate. A sensitive HPLC method was used to analyse 5 of the 6 New Zealand Bulbinella species, all of which contained phenylanthraquinones. Leaves and roots had different profiles, but species were not distinct. Roots were rich in sulphated and free phenylanthraquinones (0.27 ± 0.09% dry wt), whereas leaves typically only contained free knipholone (0.14 ± 0.01%). Localisation of phenylanthraquinones to the stele and peel was observed in roots. Two flavone-C-glucosides were found in leaves of Bulbinella., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

We apply a biopsychosocial conceptualization to attention development in the 1st year and examine the role of neurophysiological and social processes on the development of early attention processes. We tested whether maternal behavior measured during 2 mother-child interaction tasks when infants (N = 388) were 5 months predicted infant medial frontal (F3/F4) EEG power and observed attention behavior during an attention task at 10 months. After controlling for infant attention behavior and EEG power in the same task measured at an earlier 5-month time point, results indicated a significant direct and positive association from 5-month maternal positive affect to infant attention behavior at 10 months. However, maternal positive affect was not related to medial frontal EEG power. In contrast, 5-month maternal intrusive behavior was associated with infants' task-related EEG power change at the left frontal location, F3, at 10 months of age. The test of indirect effects from 5-month maternal intrusiveness to 10-month infant attention behavior via infants' EEG power change at F3 was significant. These findings suggest that the development of neural networks serving attention processes may be 1 mechanism through which early maternal behavior is related to infant attention development in the 1st year and that intrusive maternal behavior may have a particularly disruptive effect on this process. (PsycINFO Database Record, ((c) 2016 APA, all rights reserved).)

Introduction: The valuable secondary metabolites in hops (bitter acids, xanthohumol, volatile monoterpenes and sesquiterpenes) are sequestered in lupulin glands (extracellular trichomes) which can be collected and analysed with little or no sample preparation., Objectives: To determine whether high throughput screening of lupulin glands composition, by fast analyses and chemometrics, could be used for breeder selection of hops with key flavour attributes., Methods: Lupulin glands from 139 plants (39 cultivars/advanced selections) were analysed by Raman and 1 H NMR spectroscopy, and head-space solid-phase microextraction (HS-SPME) GC-FID. The digital X,Y-data were subjected to principal component analysis (PCA) and the results compared with conventional analyses of extracts of whole hops from the same plants. Quantitative 1 H NMR analyses were also done for the bitter acids., Results: Raman spectroscopy rapidly identified hops cultivars with high xanthohumol concentrations and high α:β bitter acid ratios. 1 H NMR spectroscopy was slower, requiring a solvent extraction, but distinguished cultivars by cohumulone content as well as α:β acid ratios. HS-SPME-GC rapidly distinguished aroma hops with high myrcene and farnesene contents, and pinpointed a novel selection with unusual sesquiterpenes. The quantitative NMR analyses showed correlations between bitter acid concentrations related to biosynthetic pathways., Conclusions: Analysis of lupulin glands gave reliable results for the main quality indicators used by hops breeders, potentially avoiding harvesting, drying and solvent extracting whole hops. PCA of digital X,Y-data rapidly discriminated different hops chemotypes, and highlighted plants with potential for new flavourcultivars. Copyright © 2016 John Wiley & Sons, Ltd., (Copyright © 2016 John Wiley & Sons, Ltd.)

A growing body of literature indicates that childhood emotion regulation predicts later success with peers, yet little is known about the processes through which this association occurs. The current study examined mechanisms through which emotion regulation was associated with later peer acceptance and peer rejection, controlling for earlier acceptance and rejection. Data included mother-, teacher-, and peer-reports on 338 children (55% girls, 68% European American) at ages 7 and 10. A path analysis was conducted to test the indirect effects of emotion regulation at age 7 on peer acceptance and peer rejection at age 10 via positive social behaviors of cooperation and leadership, and negative social behaviors of indirect and direct aggression. Results indicated numerous significant indirect pathways. Taken together, findings suggest cooperation, leadership, and direct and indirect aggression are all mechanisms by which earlier emotion regulation contributes to later peer status during childhood.

Theoretical perspectives of cognitive development have maintained that functional integration of the prefrontal cortex across infancy underlies the emergence of attentional control and higher cognitive abilities in early childhood. To investigate these proposed relations, we tested whether functional integration of prefrontal regions across the second half of the first year predicted observed cognitive performance in early childhood 1 year prior indirectly through observed attentional control (N = 300). Results indicated that greater change in left-but not right-frontal EEG coherence between 5 and 10 months was positively associated with attentional control, cognitive flexibility, receptive language, and behavioral inhibitory control. Specifically, a larger increase in coherence between left frontal regions was positively associated with accuracy on a visual search task at Age 2, and visual search accuracy was positively associated with receptive vocabulary, performance on a set-shifting task (DCCS), and delay of gratification at Age 3. Finally, the indirect effects from the change in left frontal EEG coherence to 3-year cognitive flexibility, receptive language, and behavioral inhibitory control were significant, suggesting that internally controlled attention is a mechanism through which early neural maturation influences children's cognitive development. (PsycINFO Database Record, ((c) 2016 APA, all rights reserved).)

Four trimethylated acylphloroglucinols (5-8) have been isolated from ma̅nuka (Leptospermum scoparium) foliage. Apart from myrigalone A (8), which has previously been isolated from European bog myrtle (Myrica gale), these compounds have not been characterized before. The nortriketones are structurally similar to the bioactive tetramethylated β-triketones from ma̅nuka, but have one less ring methyl group. Two oxidized trimethylated compounds, 9 and 10, were also isolated, but these are likely isolation artifacts. When evaluated for antibacterial activity against Gram-positive bacteria, myrigalone A (8) was slightly less potent (MIC 64 μg/mL) than the corresponding tetramethylated compound, grandiflorone (4) (MIC 16-32 μg/mL). Unlike their tetramethylated analogues, the nortriketones were inactive against the herbicide target enzyme p-hydroxyphenylpyruvate dioxygenase. The Raman spectra of leaf oil glands in different ma̅nuka varieties can be used to distinguish plants that contain nortriketones from those that accumulate triketones.

The potential indirect effect of observed maternal sensitivity at 5 months on the development of infants' behavioral regulation of emotion from 5 to 10 months (i.e., distraction and maternal-orientation behaviors) via infant's vagal withdrawal was investigated ( N = 230). Results indicated that maternal sensitivity at 5 months was not directly associated with behavioral regulation at 10 months. However, greater maternal sensitivity at 5 months was associated with greater vagal withdrawal at 10 months, after controlling for vagal withdrawal at 5 months. Further, vagal withdrawal at 10 months was associated with greater orientation toward the mother at 10 months, after controlling for 5-month orientation behaviors. The indirect effect of maternal sensitivity on maternal-orientation behaviors was significant, suggesting that infant's vagal withdrawal may be one potential mechanism through which maternal sensitivity is related to behavioral regulation.

Current theoretical conceptualizations of regulatory development suggest that attention processes and emotion regulation processes share common neurophysiological underpinnings and behavioral antecedents such that emotion regulation abilities may build on early attentional skills. To further elucidate this proposed relationship, we tested whether early neurophysiological processes measured during an attention task in infancy predicted in-task attention behavior and whether infants' attention behavior was subsequently associated with their ability to regulate emotion during early childhood (N=388). Results indicated that greater electroencephalogram (EEG) power change (from baseline to task) at medial frontal locations (F3 and F4) during an attention task at 10months of age was associated with concurrent observed behavioral attention. Specifically, greater change in EEG power at the right frontal location (F4) was associated with more attention and greater EEG power at the left frontal location (F3) was associated with less attention, indicating a potential right hemisphere specialization for attention processes already present during the first year of life. In addition, after controlling for 5-month attention behavior, increased behavioral attention at 10months was negatively associated with children's observed frustration to emotional challenge at 3years of age. Finally, the indirect effects from 10-month EEG power change at F3 and F4 to 3-year emotion regulation via infants' 10-month behavioral attention were significant, suggesting that infants' attention behavior is one mechanism through which early neurophysiological activity is related to emotion regulation abilities during childhood., (Copyright © 2015 Elsevier Inc. All rights reserved.)