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1. CT Imaging Assessment of Response to Treatment in Allergic Bronchopulmonary Aspergillosis in Adults With Bronchial Asthma

Author

Mal, Hervé, Le Guen, Pierre, Dupin, Clairelyne, Meurice, Jean Claude, Verdaguer, Marion, de Keizer, Joe, Delétage-Métreau, Céline, Le Mao, Raphael, Tromer, Cécile, Fajole, Gaëlle, Rayez, Mélanie, Raymond, Christel Saint, Gheerbrant, Hubert, Badatcheff, Anne, Person, Christine, Macey, Julie, Dermant, Xavier, Boitiaux, Jean-François, Gosset-Woimant, Marine, Metz-Favre, Carine, Degot, Tristan, Poulet, Claire, Popin, Elisabeth, Gamez, Anne Sophie, Boissin, Clément, Prevotat, Anne, Mangiapan, Gilles, Brouquières, Danielle, Le Floch, Hervé, Morisse-Pradier, Hélène, Sattler, Caroline, Muti, Daniela, Germaud, Patrick, Dirou, Stéphanie, Paris, Audrey, Melloni, Boris, Ballouhey, Julia, Bondeelle, Louise, Laurent, Lucie, Belleguic, Chantal, Kerjouan, Mallorie, Deslée, Gaëtan, Dury, Sandra, Bergot, Emmanuel, Magnier, Romain, Morel, Hugues, Lemaire, Bertrand, Tumino, Cécile, Sénéchal, Agathe, Choinier, Pascaline, Parrot, Antoine, Godet, Cendrine, Brun, Anne-Laure, Couturaud, Francis, Laurent, François, Frat, Jean-Pierre, Marchand-Adam, Sylvain, Gagnadoux, Frédéric, Blanchard, Elodie, Taillé, Camille, Philippe, Bruno, Hirschi, Sandrine, Andréjak, Claire, Bourdin, Arnaud, Chenivesse, Cécile, Dominique, Stéphane, Murris-Espin, Marlène, Rivière, Frédéric, Garcia, Gilles, Blanc, François-Xavier, Goupil, François, Bergeron, Anne, Flament, Thomas, Priou, Pascaline, Ragot, Stéphanie, and Cadranel, Jacques

Published
2024
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2. Association Between Nocturnal Hypoxemia and Cancer Incidence in Patients Investigated for OSA: Data From a Large Multicenter French Cohort

Author

Person, Christine, Priou, Pascaline, Molinier, Olivier, Paris, Audrey, Caby, Isabelle, Bellier, Maël, Langelot-Richard, Marie, Jaffre, Sandrine, Corne, Frédéric, Normand de la Tranchade, Marc, Rouault, Béatrice, Justeau, Grégoire, Gervès-Pinquié, Chloé, Le Vaillant, Marc, Trzepizur, Wojciech, Meslier, Nicole, Goupil, François, Pigeanne, Thierry, Launois, Sandrine, Leclair-Visonneau, Laurene, Masson, Philippe, Bizieux-Thaminy, Acya, Humeau, Marie-Pierre, Gosselin, Christelle, Blanchard, Margaux, Urban, Thierry, and Gagnadoux, Frédéric

Published
2020
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3. CT Imaging Assessment of Response to Treatment in Allergic Bronchopulmonary Aspergillosis in Adults With Bronchial Asthma

Author

Godet, Cendrine, primary, Brun, Anne-Laure, additional, Couturaud, Francis, additional, Laurent, François, additional, Frat, Jean-Pierre, additional, Marchand-Adam, Sylvain, additional, Gagnadoux, Frédéric, additional, Blanchard, Elodie, additional, Taillé, Camille, additional, Philippe, Bruno, additional, Hirschi, Sandrine, additional, Andréjak, Claire, additional, Bourdin, Arnaud, additional, Chenivesse, Cécile, additional, Dominique, Stéphane, additional, Mangiapan, Gilles, additional, Murris-Espin, Marlène, additional, Rivière, Frédéric, additional, Garcia, Gilles, additional, Blanc, François-Xavier, additional, Goupil, François, additional, Bergeron, Anne, additional, Flament, Thomas, additional, Priou, Pascaline, additional, Mal, Hervé, additional, de Keizer, Joe, additional, Ragot, Stéphanie, additional, Cadranel, Jacques, additional, Le Guen, Pierre, additional, Dupin, Clairelyne, additional, Meurice, Jean Claude, additional, Verdaguer, Marion, additional, Delétage-Métreau, Céline, additional, Le Mao, Raphael, additional, Tromer, Cécile, additional, Fajole, Gaëlle, additional, Rayez, Mélanie, additional, Raymond, Christel Saint, additional, Gheerbrant, Hubert, additional, Badatcheff, Anne, additional, Person, Christine, additional, Macey, Julie, additional, Dermant, Xavier, additional, Boitiaux, Jean-François, additional, Gosset-Woimant, Marine, additional, Metz-Favre, Carine, additional, Degot, Tristan, additional, Poulet, Claire, additional, Popin, Elisabeth, additional, Gamez, Anne Sophie, additional, Boissin, Clément, additional, Prevotat, Anne, additional, Brouquières, Danielle, additional, Le Floch, Hervé, additional, Morisse-Pradier, Hélène, additional, Sattler, Caroline, additional, Muti, Daniela, additional, Germaud, Patrick, additional, Dirou, Stéphanie, additional, Paris, Audrey, additional, Melloni, Boris, additional, Ballouhey, Julia, additional, Bondeelle, Louise, additional, Laurent, Lucie, additional, Belleguic, Chantal, additional, Kerjouan, Mallorie, additional, Deslée, Gaëtan, additional, Dury, Sandra, additional, Bergot, Emmanuel, additional, Magnier, Romain, additional, Morel, Hugues, additional, Lemaire, Bertrand, additional, Tumino, Cécile, additional, Sénéchal, Agathe, additional, Choinier, Pascaline, additional, and Parrot, Antoine, additional

Published
2024
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5. Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis: Results from a French Multicenter Prospective Study

Author

Coron, Noémie, Pihet, Marc, Fréalle, Emilie, Lemeille, Yolande, Pinel, Claudine, Pelloux, Hervé, Gargala, Gilles, Favennec, Loic, Accoceberry, Isabelle, Durand-Joly, Isabelle, Dalle, Frédéric, Huet, Frédéric, Fanton, Annlyse, Boldron, Amale, Loeuille, Guy-André, Domblides, Philippe, Coltey, Bérengère, Pin, Isabelle, Llerena, Catherine, Troussier, Françoise, Person, Christine, Marguet, Christophe, Wizla, Nathalie, Thumerelle, Caroline, Turck, Dominique, Bui, Stéphanie, Fayon, Michael, Duhamel, Alain, Prévotat, Anne, Wallaert, Benoit, Leroy, Sylvie, Bouchara, Jean-Philippe, and Delhaes, Laurence

Published
2017
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7. Relationship Between OSA Clinical Phenotypes and CPAP Treatment Outcomes

Author

Gagnadoux, Frédéric, Le Vaillant, Marc, Paris, Audrey, Pigeanne, Thierry, Leclair-Visonneau, Laurence, Bizieux-Thaminy, Acya, Alizon, Claire, Humeau, Marie-Pierre, Nguyen, Xuan-Lan, Rouault, Béatrice, Trzepizur, Wojciech, Meslier, Nicole, Person, Christine, Priou, Pascaline, Goupil, François, Molinier, Olivier, Breton, Philippe, Berkani, Kamel, Langelot-Richard, Marie, Chollet, Sylvaine, Jaffre, Sandrine, Corne, Frédéric, Humeau, Marie-Pierre, de la Tranchade, Marc Normand, Racineux, Jean-Louis, Gosselin, Christelle, and Pelletier-Fleury, Nathalie

Published
2016
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8. Overnight Oximetry–derived Pulse Rate Variability Predicts Stroke Risk in Patients with Obstructive Sleep Apnea

Author

Sabil, Abdelkebir, primary, Gervès-Pinquié, Chloé, additional, Blanchard, Margaux, additional, Feuilloy, Mathieu, additional, Trzepizur, Wojciech, additional, Goupil, François, additional, Pigeanne, Thierry, additional, Oger, Emmanuel, additional, Girault, Jean-Marc, additional, Gagnadoux, Frédéric, additional, Meslier, Nicole, additional, Person, Christine, additional, Priou, Pascaline, additional, Molinier, Olivier, additional, and Paris, Audrey, additional

Published
2021
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9. Rapid Improvement after Starting Elexacaftor–Tezacaftor–Ivacaftor in Patients with Cystic Fibrosis and Advanced Pulmonary Disease

Author

Burgel, Pierre-Régis, primary, Durieu, Isabelle, additional, Chiron, Raphaël, additional, Ramel, Sophie, additional, Danner-Boucher, Isabelle, additional, Prevotat, Anne, additional, Grenet, Dominique, additional, Marguet, Christophe, additional, Reynaud-Gaubert, Martine, additional, Macey, Julie, additional, Mely, Laurent, additional, Fanton, Annlyse, additional, Quetant, Sébastien, additional, Lemonnier, Lydie, additional, Paillasseur, Jean-Louis, additional, Da Silva, Jennifer, additional, Martin, Clémence, additional, Andrejak, Claire, additional, Becourt, Arnaud, additional, Mounard, Julie, additional, Poulet, Claire, additional, Rames, Cinthia, additional, Talleux, Marie, additional, Chevalier, Marie-Chantal, additional, Darviot, Estelle, additional, Jouvenot, Marie, additional, Marien, Caroline, additional, Paris, Audrey, additional, Pelatan, Cécile, additional, Person, Christine, additional, Priou, Pascaline, additional, Troussier, Françoise, additional, Urban, Thierry, additional, Dalphin, Marie-Laure, additional, Dalphin, Jean-Charles, additional, Ladaurade, Alice, additional, Pernet, Didier, additional, Richaud-Thiriez, Bénédicte, additional, Roux-Claude, Pauline, additional, Blanc, Nathalia, additional, Boisserie-Lacroix, Vincent, additional, Bui, Stephanie, additional, Collet, Cyrielle, additional, Debelleix, Stéphane, additional, Bergot, Emmanuel, additional, Brouard, Jacques, additional, Campbell, Karine, additional, Laurans, Muriel, additional, Ribault, Virginie, additional, Borderon, Corinne, additional, Heraud, Marie-Christine, additional, Labbe, Guillaume, additional, Montcouquiol, Sylvie, additional, Petit, Isabelle, additional, Ruivard, Marc, additional, Delestrain, Céline, additional, Douvry, Benoit, additional, Epaud, Ralph, additional, Maitre, Bernard, additional, Remus, Natascha, additional, Beltramo, Guillaume, additional, Houzel, Anne, additional, Huet, Frédéric, additional, Perez, Stéphanie, additional, Boldron-Ghaddar, Amale, additional, Scalbert, Manuela, additional, Bouzioukh, Rabah, additional, Simon, Charles, additional, Camara, Boubou, additional, Hamidfar, Rébecca, additional, Llerena, Catherine, additional, Pin, Isabelle, additional, Deschildre, Antoine, additional, Gicquello, Alice, additional, Le Rouzic, Olivier, additional, Leroy, Clara, additional, Paris, Nicolas, additional, Perez, Thierry, additional, Thumerelle, Caroline, additional, Turck, Dominique, additional, Wizla, Nathalie, additional, Dupuy-Grasset, Magali, additional, Languepin, Jane, additional, Masson-Rouchaud, Alexandra, additional, Menetrey, Céline, additional, Durupt, Stéphane, additional, L’Excellent, Sophie, additional, Nove-Josserand, Raphaele, additional, Ohlmann, Camille, additional, Reix, Phillipe, additional, Reynaud, Quitterie, additional, Werck-Gallois, Marie-Christine, additional, Baravalle, Mélissandre, additional, Coltey, Bérangère, additional, Desmazes-Dufeu, Nadine, additional, Dubus, Jean-Christophe, additional, Gautier, Clarisse, additional, Rey, Jean-Baptiste, additional, Stremler, Nathalie, additional, Caimmi, Davide, additional, Devrait, Margot, additional, Moreau, Johan, additional, Billon, Yves, additional, Blondé, Aurore, additional, Guillaumot, Anne, additional, Kiefer, Sébastien, additional, Peretti, Laura, additional, Tatopoulos, Aurélie, additional, Tiotiu, Angélica, additional, Benhamida, Myriam, additional, Bihouee, Tiphaine, additional, Eschapasse, Emmanuel, additional, Tissot, Adrien, additional, Giannantonio, Marie, additional, Leroy, Sylvie, additional, Piccini-Bailly, Carole, additional, Pradelli, Johana, additional, Messika, Jonathan, additional, Boussaud, Véronique, additional, Burgel, Pierre-Régis, additional, Carlier, Nicolas, additional, Honoré, Isabelle, additional, Hubert, Dominique, additional, Kanaan, Reem, additional, Bailly-Botuha, Céline, additional, Chedevergne, Frédérique, additional, De Blic, Jacques, additional, Delacourt, Christophe, additional, Drummond, David, additional, Fauroux, Brigitte, additional, Karila, Chantal, additional, Le Bourgeois, Muriel, additional, Sermet, Isabelle, additional, Delaisi, Bertrand, additional, Gerardin, Michèle, additional, Houdouin, Veronique, additional, Leclainche, Laurence, additional, Aubertin, Guillaume, additional, Berdah, Laura, additional, Clement, Annick, additional, Corvol, Harriet, additional, Nathan, Nadia, additional, Prevost, Blandine, additional, Richard, Nicolas, additional, Tamalet, Aline, additional, Taytard, Jessica, additional, Thouvenin, Guillaume, additional, Tourniaire, Barbara, additional, Abely, Michel, additional, Bessaci-Kabouya, Katia, additional, Dury, Sandra, additional, Ravoninjatovo, Bruno, additional, Dabadie, Alain, additional, Dagorne, Michel, additional, Deneuville, Eric, additional, Jamin, Marie, additional, Ribault, Mélanie, additional, Vigier, Clémentine, additional, Belleguic, Chantal, additional, Brinchault, Graziella, additional, Desrues, Benoit, additional, Barzic, Audrey, additional, Dirou-Prigent, Anne, additional, Le Bihan, Jean, additional, Revert, Krista, additional, Ropars, Thomas, additional, Couderc, Laure, additional, Dominique, Stéphane, additional, Morisse-Pradier, Hélène, additional, Pramil, Stéphanie, additional, Thiberville, Luc, additional, Allou, Nathalie, additional, Enaud, Laurent, additional, Gachelin, Elsa, additional, Huchot, Eric, additional, Payet, Annabelle, additional, Perisson, Caroline, additional, Piyaraly, Saguiraly, additional, Valois, Sophie, additional, Herzog, Audrey, additional, Kessler, Romain, additional, Porzio, Michele, additional, Weiss, Laurence, additional, Beaumont, Laurence, additional, Brugiere, Olivier, additional, Colin, Sylvie, additional, Verdiere, de, additional, Cuquemelle, Elise, additional, De Miranda, Sandra, additional, Monem Hamid, Adbdul, additional, Parquin, François, additional, Picard, Clément, additional, Roux, Antoine, additional, Roy, Charlotte, additional, Bremont, François, additional, Didier, Alain, additional, Dupuis, Marion, additional, Faviez, Guillaume, additional, Labouret, Géraldine, additional, Mittaine, Marie, additional, Murris-Espin, Marlène, additional, Roditis, Léa, additional, Cosson, Laure, additional, Diot, Patrice, additional, Flament, Thomas, additional, Giraut, Charlotte, additional, Mankikian, Julie, additional, Arnouat, Baptiste, additional, Mousset, Gaétane, additional, Storni, Véronique, additional, Vigneron, Philippe, additional, Coirier-Duet, Emmanuelle, additional, and Gabsi, Asma, additional

Published
2021
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10. Association Between Nocturnal Hypoxemia and Cancer Incidence in Patients Investigated for OSA

Author

Justeau, Grégoire, primary, Gervès-Pinquié, Chloé, additional, Le Vaillant, Marc, additional, Trzepizur, Wojciech, additional, Meslier, Nicole, additional, Goupil, François, additional, Pigeanne, Thierry, additional, Launois, Sandrine, additional, Leclair-Visonneau, Laurene, additional, Masson, Philippe, additional, Bizieux-Thaminy, Acya, additional, Humeau, Marie-Pierre, additional, Gosselin, Christelle, additional, Blanchard, Margaux, additional, Urban, Thierry, additional, Gagnadoux, Frédéric, additional, Person, Christine, additional, Priou, Pascaline, additional, Molinier, Olivier, additional, Paris, Audrey, additional, Caby, Isabelle, additional, Bellier, Maël, additional, Langelot-Richard, Marie, additional, Jaffre, Sandrine, additional, Corne, Frédéric, additional, Normand de la Tranchade, Marc, additional, and Rouault, Béatrice, additional

Published
2020
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11. Real-Life Safety and Effectiveness of Lumacaftor–Ivacaftor in Patients with Cystic Fibrosis

Author

Burgel, Pierre-Régis, primary, Munck, Anne, additional, Durieu, Isabelle, additional, Chiron, Raphaël, additional, Mely, Laurent, additional, Prevotat, Anne, additional, Murris-Espin, Marlene, additional, Porzio, Michele, additional, Abely, Michel, additional, Reix, Philippe, additional, Marguet, Christophe, additional, Macey, Julie, additional, Sermet-Gaudelus, Isabelle, additional, Corvol, Harriet, additional, Bui, Stéphanie, additional, Lemonnier, Lydie, additional, Dehillotte, Clémence, additional, Da Silva, Jennifer, additional, Paillasseur, Jean-Louis, additional, Hubert, Dominique, additional, Mounard, Julie, additional, Poulet, Claire, additional, Rames, Cinthia, additional, Person, Christine, additional, Troussier, Françoise, additional, Urban, Thierry, additional, Dalphin, Marie-Laure, additional, Dalphin, Jean-Claude, additional, Pernet, Didier, additional, Richaud-Thiriez, Bénédicte, additional, Fayon, Mickael, additional, Macey-Caro, Julie, additional, Campbell, Karine, additional, Laurans, Muriel, additional, Borderon, Corinne, additional, Heraud, Marie-Christine, additional, Labbé, André, additional, Montcouquiol, Sylvie, additional, Bassinet, Laurence, additional, Remus, Natascha, additional, Fanton, Annlyse, additional, Houzel-Charavel, Anne, additional, Huet, Frédéric, additional, Perez-Martin, Stéphanie, additional, Boldron-Ghaddar, Amale, additional, Scalbert, Manuela, additional, Camara, Boubou, additional, Llerena, Catherine, additional, Pin, Isabelle, additional, Quétant, Sébastien, additional, Cottereau, Aurélie, additional, Deschildre, Antoine, additional, Gicquello, Alice, additional, Perez, Thierry, additional, Stervinou-Wemeau, Lidwine, additional, Thumerelle, Caroline, additional, Wallaert, Benoit, additional, Wizla, Nathalie, additional, Languepin, Jane, additional, Ménétrey, Céline, additional, Dupuy-Grasset, Magalie, additional, Bazus, Lucie, additional, Buchs, Clelia, additional, Jubin, Virginie, additional, Werck-Gallois, Marie-Christine, additional, Mainguy, Catherine, additional, Perrin, Thomas, additional, Toutain-Rigolet, Agnès, additional, Durupt, Stéphane, additional, Reynaud, Quitterie, additional, Nove-Josserand, Raphaele, additional, Baravalle-Einaudi, Melisande, additional, Coltey, Bérangère, additional, Dufeu, Nadine, additional, Dubus, Jean-Christophe, additional, Stremler, Nathalie, additional, Caimmi, Davide, additional, Billon, Yves, additional, Derelle, Jocelyne, additional, Kieffer, Sébastien, additional, Pichon, Anne-Sophie, additional, Schweitzer, Cyril, additional, Tatopoulos, Aurélie, additional, Abbes, Sarah, additional, Bihouée, Tiphaine, additional, Danner-Boucher, Isabelle, additional, David, Valérie, additional, Haloun, Alain, additional, Tissot, Adrien, additional, Leroy, Sylvie, additional, Bailly-Piccini, Carole, additional, Clément, Annick, additional, Tamalet, Aline, additional, Burgel, Pierre-Régis, additional, Honoré, Isabelle, additional, Kanaan, Reem, additional, Martin, Clémence, additional, Bailly, Cécile, additional, Chédevergne, Frédérique, additional, De Blic, Jacques, additional, Fauroux, Brigitte, additional, Le Bourgeois, Murielle, additional, Delaisi, Bertrand, additional, Gérardin, Michèle, additional, Abély, Michel, additional, Ravoninjatovo, Bruno, additional, Belleguic, Chantal, additional, Desrues, Benoit, additional, Brinchault, Graziella, additional, Dagorne, Michel, additional, Deneuville, Eric, additional, Lefeuvre, Sylvaine, additional, Dirou, Anne, additional, Le Bihan, Jean, additional, Ramel, Sophie, additional, Dominique, Stéphane, additional, Payet, Annabelle, additional, Kessler, Romain, additional, Rosner, Vincent, additional, Weiss, Laurence, additional, de Miranda, Sandra, additional, Grenet, Dominique, additional, Hamid, Abdoul, additional, Picard, Clément, additional, Brémont, François, additional, Didier, Alain, additional, Labouret, Géraldine, additional, Mittaine, Marie, additional, Murris-Espin, Marlène, additional, Têtu, Laurent, additional, Cosson, Laure, additional, Giraut, Charlotte, additional, Henriet, Anne-Cécile, additional, Mankikian, Julie, additional, Marchand, Sophie, additional, Hugé, Sandrine, additional, Storni, Véronique, additional, and Coirier-Duet, Emmanuelle, additional

Published
2020
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12. Toward the Standardization of Mycological Examination of Sputum Samples in Cystic Fibrosis : Results from a French Multicenter Prospective Study

Author

Lafarge, Céline, Pontoire, Bruno, Cayot, Nathalie, Le Bail, Patricia, Coron, Noémie, Pihet, Marc, Fréalle, Emilie, Lemeille, Yolande, Pinel, Claudine, Pelloux, Hervé, Gargala, Gilles, Favennec, Loïc, Accoceberry, Isabelle, Durand-Joly, Isabelle, Dalle, Frédéric, Huet, Frédéric, Fanton, Annlyse, Boldron, Amale, Loeuille, Guy-André, Domblides, Philippe, Coltey, Bérengère, Pin, Isabelle, Llerena, Catherine, Troussier, Françoise, Person, Christine, Marguet, Christophe, Wizla, Nathalie, Thumerelle, Caroline, Turck, Dominique, Bui, Stéphanie, Fayon, Michael, Duhamel, Alain, Prévotat, Anne, Leroy, Sylvie, Wallaert, Benoit, Bouchara, Jean-Philippe, Delhaes, Laurence, Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ATOMycA (CRCINA-ÉQUIPE 6), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Laboratoire de Parasitologie et Mycologie (Parasito - Myco - LILLE), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université Grenoble Alpes - UFR Pharmacie (UGA UFRP), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Pôle Biologie, Institut de Biologie et Pathologie, Centre Hospitalier Universitaire de Grenoble, Laboratoire de parasitologie-mycologie, CHU Grenoble, CHU Rouen, Normandie Université (NU), Appareil Digestif Environnement Nutrition (ADEN ), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interactions Muqueuses Agents Transmissibles (LIMA), Université de Bourgogne (UB), Service de pédiatrie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre de Ressources et de Compétences de la Mucoviscidose [CHU Dijon] (CRCM ), CHU Bordeaux, Ctr Rech Mucoviscidose CRCM Adulte, CHU Bordeaux [Bordeaux], CRCM Adulte [Grenoble], Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pédiatrie générale et maladies infectieuses, Service de Pneumologie Pédiatrique [Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Centre Robert Debré, PRES Université Nantes Angers Le Mans (UNAM), Service de pédiatrie médicale et médecine de l'adolescent [Rouen], Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Clinique de Pédiatrie, Hôpital Jeanne de Flandre [Lille]-Université de Lille, Hôpital Jeanne de Flandre [Lille], Pediatrics, CHU Bordeaux, Dept Pediat CRCM, INSERM Ctr Invest Clin CIC5, Université de Bordeaux (UB)-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Cardio-thoracique de Bordeaux, U1045, Institut d'Informatique et de Mathématiques Appliquées de Grenoble (IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS), Département de Pneumologie, Hôpital A. Calmette, Centre Hospitalier Universitaire de Nice (CHU de Nice), Service de Pneumologie et Immuno-Allergologie [CHU LIlle], Pole Cardio-vasculaire et pulmonaire [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de Parasitologie et de Mycologie [Lille], Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB), Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM), French Ministry of Health and Research (PHRC N°2006/1902), the association 'Vaincre la Mucoviscidose' (Defeat Cystic Fibrosis) (MucoFong and Mucofong-ATF N8 2006/351), the Pharmaceutical Division of Pfizer France (Nu 2006/158), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), FLAveur, VIsion et Comportement du consommateur (FLAVIC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), ATIP AVENIR / ATOMycA (CRCINA - Département INCIT - Equipe 6), Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers (CRCINA), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Groupe d'Etude des Interactions Hôte-Parasite (GEIHP), Université Grenoble Alpes (UGA), Service de Pédiatrie, Centre de Ressources et de Compétences de la Mucoviscidose [Dijon], Service de Pneumologie et Immuno-Allergologie, Centre hospitalier universitaire de Nantes (CHU Nantes)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes (UN)-Université d'Angers (UA)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes (UN)-Université d'Angers (UA), Service de Parasitologie-Mycologie [CHRU LIlle], Institut de Microbiologie [CHRU Lille], Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Universitaire [Grenoble] (CHU), université de Bourgogne, PAM, Hôpital Charles Nicolle [Rouen], Université de Bordeaux (UB), CH Dunkerque, Centre de Recherche en Chimie Moléculaire (CRCM), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Groupe de Recherche sur les Antimicrobiens et les Micro-Organismes (GRAM 1.0), CHU Lille, CIC Bordeaux, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Etudes et de Recherche en Informatique Médicale [Lille] (CERIM), Hôpital Pasteur [Nice] (CHU), Centre Hospitalier Universitaire de Nice (CHU Nice), Procédés Alimentaires et Microbiologiques [Dijon] ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Unité de recherche sur les Biopolymères, Interactions Assemblages ( BIA ), Institut National de la Recherche Agronomique ( INRA ), FLAveur, VIsion et Comportement du consommateur ( FLAVIC ), Etablissement National d'Enseignement Supérieur Agronomique de Dijon ( ENESAD ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ), ATIP AVENIR / ATOMycA ( CRCINA - Département INCIT - Equipe 6 ), Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers ( CRCINA ), Université d'Angers ( UA ) -Université de Nantes ( UN ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche en Santé de l'Université de Nantes ( IRS-UN ) -Centre hospitalier universitaire de Nantes ( CHU Nantes ) -Université d'Angers ( UA ) -Université de Nantes ( UN ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche en Santé de l'Université de Nantes ( IRS-UN ) -Centre hospitalier universitaire de Nantes ( CHU Nantes ), Groupe d'Etude des Interactions Hôte-Parasite ( GEIHP ), Université d'Angers ( UA ), Laboratoire de Parasitologie et Mycologie ( Parasito - Myco - LILLE ), Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ), Université Grenoble Alpes - UFR Pharmacie ( UGA UFRP ), Université Grenoble Alpes ( UGA ), Appareil Digestif Environnement Nutrition ( ADEN ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Microbiologie cellulaire et moléculaire et pathogénicité ( MCMP ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Interactions Muqueuses Agents Transmissibles ( LIMA ), Université de Bourgogne ( UB ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Institut d'oncologie/développement Albert Bonniot de Grenoble ( INSERM U823 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Universitaire d'Angers-Centre Robert Debré, Centre Hospitalier Universitaire d'Angers ( CHU Angers ), PRES Université Nantes Angers Le Mans ( UNAM ), CHU Rouen-Université de Rouen Normandie ( UNIROUEN ), Université de Bordeaux ( UB ) -CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Universitaire de Bordeaux, Institut d'Informatique et de Mathématiques Appliquées de Grenoble ( IMAG ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National Polytechnique de Grenoble ( INPG ) -Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Nice ( CHU de Nice ), Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] ( CRCTB ), and Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale ( INSERM )

Subjects
Adult, Male, Microbiological Techniques, 0301 basic medicine, medicine.medical_specialty, Filamentous fungi, Adolescent, Veterinary (miscellaneous), Lung mycobiota, 030106 microbiology, Biology, Applied Microbiology and Biotechnology, Microbiology, Cystic fibrosis, Aspergillus fumigatus, Molds, Young Adult, 03 medical and health sciences, Medical microbiology, Exophiala, Mycological examination, medicine, Humans, Prospective Studies, Child, Candida albicans, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged, Aged, 80 and over, Aspergillus, Lung Diseases, Fungal, Fungi, Sputum, Scedosporium apiospermum, Middle Aged, biology.organism_classification, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Corpus albicans, 030104 developmental biology, Female, France, medicine.symptom, Agronomy and Crop Science
Abstract

IF 1.710; International audience; Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: “MucoFong” program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media. After mucolytic pretreatment, sputa were plated in parallel on cycloheximide-enriched (ACT37), erythritol-enriched (ERY37), benomyl dichloran–rose bengal (BENO37) and chromogenic (CAN37) media incubated at 37 °C and on Sabouraud–chloramphenicol (SAB27) and erythritol-enriched (ERY27) media incubated at 20–27 °C. Each plate was checked twice a week during 3 weeks. Fungi were conventionally identified; time for detection of fungal growth was noted for each species. Fungal prevalences and media performances were assessed; an optimal combination of media was determined using the Chi-squared automatic interaction detector method. At least one fungal species was isolated from 81% of sputa. Candida albicans was the most prevalent species (58.8%), followed by Aspergillus fumigatus (35.4%). Cultivation on CAN37, SAB27, ACT37 and ERY27 during 16 days provided an optimal combination, detecting C. albicans, A. fumigatus, Scedosporium apiospermum complex and Exophiala spp. with sensitivities of 96.5, 98.8, 100 and 100%. Combination of these four culture media is recommended to ensure the growth of key fungal pathogens in CF respiratory specimens. The use of such consensual protocol is of major interest for merging results from future epidemiological studies.

Published
2018
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13. Relationship Between OSA Clinical Phenotypes and CPAP Treatment Outcomes

Author

Person, Christine, Priou, Pascaline, Goupil, François, Molinier, Olivier, Breton, Philippe, Berkani, Kamel, Langelot-Richard, Marie, Chollet, Sylvaine, Jaffre, Sandrine, Corne, Frédéric, Humeau, Marie-Pierre, de la Tranchade, Marc Normand, Racineux, Jean-Louis, Gosselin, Christelle, Pelletier-Fleury, Nathalie, Gagnadoux, Frédéric, Le Vaillant, Marc, Paris, Audrey, Pigeanne, Thierry, Leclair-Visonneau, Laurence, Bizieux-Thaminy, Acya, Alizon, Claire, Nguyen, Xuan-Lan, Rouault, Béatrice, Trzepizur, Wojciech, and Meslier, Nicole

Published
2016
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14. Association Between Severity of Obstructive Sleep Apnea and Blood Markers of Liver Injury

Author

Trzepizur, Wojciech, primary, Boursier, Jérôme, additional, Mansour, Yasmina, additional, Le Vaillant, Marc, additional, Chollet, Sylvaine, additional, Pigeanne, Thierry, additional, Bizieux-Thaminy, Acya, additional, Humeau, Marie-Pierre, additional, Alizon, Claire, additional, Goupil, François, additional, Meslier, Nicole, additional, Priou, Pascaline, additional, Calès, Paul, additional, Gagnadoux, Frédéric, additional, Person, Christine, additional, Molinier, Olivier, additional, Paris, Audrey, additional, Caby, Isabelle, additional, Bellier, Maël, additional, Langelot-Richard, Marie, additional, Leclair-Visonneau, Laurence, additional, Jaffre, Sandrine, additional, Corne, Frédéric, additional, de la Tranchade, Marc Normand, additional, Rouault, Béatrice, additional, Racineux, Jean-Louis, additional, Gosselin, Christelle, additional, and Pelletier-Fleury, Nathalie, additional

Published
2016
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15. Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Author

Mouhajir, Abdelmounaim, primary, Matray, Olivier, additional, Giraud, Sandrine, additional, Mély, Laurent, additional, Marguet, Christophe, additional, Sermet-Gaudelus, Isabelle, additional, Le Gal, Solène, additional, Labbé, Franck, additional, Person, Christine, additional, Troussier, Françoise, additional, Ballet, Jean-Jacques, additional, Gargala, Gilles, additional, Zouhair, Rachid, additional, Bougnoux, Marie-Elisabeth, additional, Bouchara, Jean-Philippe, additional, and Favennec, Loïc, additional

Published
2016
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16. Chronic Aspergillus fumigatus colonization of respiratory tract in Cystic Fibrosis: Diagnosis, management and antifungal resistance in a French cohort of CF patients

Author

Delhaès, Laurence, Jourdain, A., d'Almeida-Fourquet, M., Fréalle, Emilie, Leroy, Sylvie, Pinel, Claudine, Coltey, Bérengère, Favennec, Loïc, Dominique, S., Vagner, O., Fanton, Annlyse, Bouchara, Jean-Philippe, Person, Christine, Durand-Joly, Isabelle, Loeuille, Guy-André, Domblides, Philippe, Accoceberry, Isabelle, Farce, A., Chavatte, P., Wallaert, Benoit, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), and Université d'Angers (UA)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Titre abrégé : Chronic Aspergillus fumigatus colonisation of respiratory tract in cystic fibrosis: diagnosis, management and antifungal resistance in a French cohort of cystic fibrosis patients; International audience; Introduction:Cystic fibrosis (CF) is the major genetic inherited diseasein the European Caucasian population, with an average of 1 in 3000 living births in France. Prognostic depend essentially on the lung impairments. While considerable attention therefore has been paid over recent decades to prevent and treat bacterial respiratory infections, we observed emergence of fungi colonization in CF respiratory tract. In particular, Aspergillus fumigatus represents the most common causative agent colonizing the airways of CF patients; it can be responsible for Allergic Bronchopulmonary Aspergillosis (ABPA). Sinceoral corticosteroids and itraconazole represent the mainstay of ABPA treatment, long-term therapy may increase the risk of acquired resistance to azoles that is mainly associated with amino acid substitutions in the CYP51A gene of A. fumigatus.Objective: Because CF patients are chronically exposed to itraconazole, our study aimed to evaluate the prevalence of azole resistance in isolates prospectively collected from CF patients followed-up in seven French hospitals involved in our national prospective study (“MucoFong” study − PHRC1902). To our knowledge, it is the first multicenter studyfocused on azole resistance of A. fumigatus in CF.Methods: A total of 87 isolates of A. fumigatus was collected in 85 patients. The MICs of azole drugs were evaluated for each isolate using the E-test ® strips. Isolates were characterized at the molecular level by targeting ITS, b-tubulin and MAT-A/a genes. The cyp51A gene as well as its promoter was sequenced.Results and Discussion:A majority of isolates (88.1%) were found sensitive to itraconazole (MIC 2 mg/ml), and 2 new mutations were identified and localized within 3-dimensional Cyp51A protein model. To obtain insight into azole resistance of A. fumigatus, the results are analyzed taking into account clinical data, itraconazole exposition, and the potential correlation between the identified CYP5IA mutations and azole resistance is discussed based on the Cyp51A protein homology model.

Published
2011
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17. Fungal colonization in Cystic Fibrosis (CF): Epidemiology and antifungal resistance in a French cohort of CF patients – Focused on Aspergillus fumigatus colonization

Author

Delhaès, Laurence, d'Almeida-Fourquet, M., Fréalle, Emilie, Leroy, Stéphane, Pinel, Claudine, Llerana, C., Favennec, Loïc, Dominique, S., Vagner, O., Fanton, Annlyse, Bouchara, Jean-Philippe, Person, Christine, Durand-Joly, Isabelle, Loeuille, Guy-André, Domblides, Philippe, Accoceberry, Isabelle, Farce, A., Chavatte, P., Wallaert, Benoit, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), and Université d'Angers (UA)

Subjects
Exophiala spp, Scedosporium apiospermum, Aspergillus fumigatus, [SDV]Life Sciences [q-bio], antifungal resistance, Cystic fibrosis, Scedosporium prolificans
Abstract

International audience; Introduction: Cystic fibrosis (CF) is the major genetic inherited disease in the European Caucasian population, with an average of 1 in 3000 living births in France. Prognostic dependessentially on the lung impairments. While considerable attention therefore has been paid over recent decades to prevent and treat bacterial respiratory infections, we observed emergence of fungi colonization in CF respiratory tract. In particular, Aspergillus fumigatus represents the most common causative agent colonizing the airways of CF patients; it can be responsible for Allergic Bronchopulmonary Aspergillosis (ABPA). Since oral corticosteroids and itraconazole represent the mainstay of ABPA treatment, long-term therapy may increase the risk of acquired resistance to azoles that is mainly associated with amino acid substitutions in the CYP51A gene of A. fumigatus.Objective: First, we managed to have exhaustive epidemiological data on species of filamentous fungi able to colonize the airway tract of 300 CF patients followed-up in our national prospective study ("MucoFong" study – PHRC1902). Second, CF patients being chronically exposed to azole (especially to itraconazole), our study aimed to evaluate the prevalence of azole resistance in isolates prospectively collected from CF patients followed-up in seven French hospitals involved in our national prospective study. Third, we focused on the most prevalent species: Aspergillus fumigatus, studying the azole resistance at molecular level. To our knowledge, it is the first multicenter study focused on azole resistance of A. fumigatus in CF.Methods:A total of 243 sputa were analyzed using the same protocol in each centre. TheMICs of antifungal drugs were evaluated for each isolate using the E-test ® strips. Focusing on A. fumigatus, a total of 87 isolates was collected in 85 patients. These isolates were characterized at the molecular level by targeting ITS, ß-tubulin and MAT-A/α genes. The CYP51A gene as well as its promoter was sequenced; a 3D Cyp51A protein homology model was built.Results and discussion:300 patients were enrolled in this study. At inclusion time, most of them were adults colonized with A. fumigatus (about 35% of the patients). Scedosporium was isolated in 5%, and Exophiala in about 2%. Regarding antifungal susceptibility, isolates of Scedosporium and Exophiala exhibited antifungal resistance comparable with published data. Regarding A. fumigatus, a majority of isolates (88.1%) were found sensitive to itraconazole (MIC≤ 2μg/ml), and 2 new mutations were identified and localized within 3-dimensional Cyp51A protein model. To obtain insight into azole resistance of A. fumigatus, the results are analyzed taking into account clinical data, itraconazole exposition, and the potential correlation between the identified CYP5IA mutations and azole resistance is discussed based on the Cyp51A protein homology model.

Published
2011

18. Role of PTX3 in Cystic Fibrosis-associated infections

Author

Hamon, Yveline, Person, Christine, Giniès, Jean-Louis, Bouchara, Jean-Philippe, Delneste, Yves, Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Innate Immunity and Immunotherapy (CRCINA-ÉQUIPE 7), and Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience; PTX3, a soluble innate immunity receptor, binds to selected microbes and facilitates theirclearance by phagocytes. PTX3 selectively binds to Pseudomonas aeruginosa and Aspergillus fumigatus, two microorganisms frequently colonizing the airways of patients with cystic fibrosis (CF), and sometimes causing true respiratory infections. PTX3 -/- mice are sensitive to A. fumigatus infection, highlighting the role of this protein in the protection against this pathogen. We thus hypothesized that PTX3 could be altered in CF patients and that this could be responsible, at least in part, to their susceptibility to some opportunistic pathogens.Serum and sputum samples from 30 CF patients (20 adults and 15 children) and 7 patientswith chronic obstructive pulmonary disease (COPD) as the control group were analyzed forPTX3 expression and integrity by ELISA and Western-blotting, respectively. The role ofendogenous or microbial proteases on recombinant human PTX3 was also analyzed.Results showed that PTX3 level was increased in CF and COPD serum, highlighting theirinfectious/inflammatory status, while, in contrast, PTX3 concentration was lower orundetectable in CF sputum than in COPD. Western-blotting showed that PTX3 is degraded in sputum samples from most of CF patients, but not in clinical specimens from COPD patients. The degradation of PTX3 was shown to be mediated by serine proteases. More precisely, both the neutrophil elastase and the alkaline proteinase from A. fumigatus have the ability to degrade in vitro PTX3.This study which shows that PTX3 is degraded in respiratory secretions from CF patients,provide new insights into the pathogenesis of microbial colonization of the airways andrespiratory infections in CF patients, since degradation of PTX3 could be responsible, at least in part, for the sensitivity of CF patients to some opportunistic infections.

Published
2011

19. Risque fongique chez les patients atteints de mucoviscidose : premier bilan de l’etude nationale multicentrique - PHRC 1902 'MUCOFONG'

Author

Delhaès, Laurence, Fréalle, Emilie, Lemeille, Yolande, Coltey, Bérengère, Gargala, Gilles, Dominique, S., Accoceberry, Isabelle, Domblides, Philippe, Durand-Joly, Isabelle, Loeuille, Guy-André, Vagner, O., Dalle, Frédéric, Fanton, Annlyse, Boldron, Amale, Pinel, Claudine, Llerena, Catherine, Pihet, Marc, Giniès, J.-L., Bouchara, Jean-Philippe, Person, Christine, Wizla, Nathalie, Thumerelle, Caroline, Favennec, Loïc, Marguet, Christophe, Bui, Stéphanie, Leroy, Sylvie, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), and Université d'Angers (UA)

Subjects
[SDV]Life Sciences [q-bio]
Published
2010

20. Exophiala dermatitidis Revealing Cystic Fibrosis in Adult Patients with Chronic Pulmonary Disease.

Author

Grenouillet, Frédéric, Cimon, Bernard, Pana-Katatali, Heloise, Person, Christine, Gainet-Brun, Marie, Malinge, Marie-Claire, Le Govic, Yohann, Richaud-Thiriez, Bénédicte, and Bouchara, Jean-Philippe

Abstract

Cystic fibrosis (CF) is a genetic inherited disease due to mutations in the gene cystic fibrosis transmembrane conductance regulator ( CFTR). Because of the huge diversity of CFTR mutations, the CF phenotypes are highly heterogeneous, varying from typical to mild form of CF, also called atypical CF. These atypical features are more frequently diagnosed at adolescence or adulthood, and among clinical signs and symptoms leading to suspect a mild form of CF, colonization or infection of the respiratory tract due to well-known CF pathogens should be a warning signal. Exophiala dermatitidis is a melanized dimorphic fungus commonly detected in respiratory specimens from CF patients, but only very rarely from respiratory specimens from non-CF patients. We described here two cases of chronic colonization of the airways by E. dermatitidis, with recurrent pneumonia and hemoptysis in one patient, which led clinicians to diagnose mild forms of CF in these elderly patients who were 68- and 87-year-old. These cases of late CF diagnosis suggest that airway colonization or respiratory infections due to E. dermatitidis in patients with bronchiectasis should led to search for a mild form of CF, regardless of the age and associated symptoms. On a broader level, in patients with chronic respiratory disease and recurrent pulmonary infections, an allergic bronchopulmonary mycosis or an airway colonization by CF-related fungi like E. dermatitidis or some Aspergillus, Scedosporium or Rasamsonia species, should be considered as potential markers of atypical CF and should led clinicians to conduct investigations for CF diagnosis. [ABSTRACT FROM AUTHOR]

Published
2018
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21. Proteolytic cleavage of the long pentraxin PTX3 in the airways of cystic fibrosis patients

Author

Hamon, Yveline, primary, Jaillon, Sébastien, additional, Person, Christine, additional, Giniès, Jean-Louis, additional, Garo, Erwan, additional, Bottazzi, Barbara, additional, Ghamrawi, Sarah, additional, Urban, Thierry, additional, Subra, Jean-François, additional, Bouchara, Jean-Philippe, additional, Mantovani, Alberto, additional, Jeannin, Pascale, additional, and Delneste, Yves, additional

Published
2013
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23. Long-Term Rasamsonia argillaceaComplex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Author

Mouhajir, Abdelmounaim, Matray, Olivier, Giraud, Sandrine, Mély, Laurent, Marguet, Christophe, Sermet-Gaudelus, Isabelle, Le Gal, Solène, Labbé, Franck, Person, Christine, Troussier, Françoise, Ballet, Jean-Jacques, Gargala, Gilles, Zouhair, Rachid, Bougnoux, Marie-Elisabeth, Bouchara, Jean-Philippe, and Favennec, Loïc

Abstract

ABSTRACTThe aim of this work was to document molecular epidemiology of Rasamsonia argillaceaspecies complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticolawas the most common species, followed by R. argillacea sensu strictoand R. piperina, while R. eburneawas not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticolaand R. argillaceaisolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillaceaspecies complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.

Published
2016
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