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1. In vitro germination and reserve mobilization of Vriesea friburgensis Mez

Author

Corredor-Prado, J. P., Santos-Amaya, O. F., and Pescador, R.

Subjects
endosperm, embrião, endosperma, plântula, embryo, cotyledon, seedling, cotilédone, semente, seed
Abstract

Studies on the germination and establishment of plants are key pieces to understanding the reproductive success of plants. This work aimed to describe in vitro germination and reserve mobilization in the bromeliad Vriesea friburgensis through morphological, histochemical, and biochemical analysis. The conditions used in this study for the in vitro germination are adequate. From the third day of in vitro inoculation, a uniform germination of 98% was obtained, exhibiting a high physiological quality of the seeds and a high potential to produce seedlings (94%). There is early reserve mobilization, which began in the imbibition phase. The accumulated reserves in the endosperm cytoplasm are degraded by hydrolytic enzymes provided by the aleurone layer. It is possible that compounds in the cell walls of the endosperm contribute to a lesser extent in mobilization. Additionally, it was observed that starch accumulation in the cotyledon increases when the seedling has formed. Results from this study provide insights for future studies on ecology, seed technology, and conservation in this species. This study contributes to the limited knowledge of the dynamics of reserves during germination and seedling establishment in Bromeliaceae. To the best of our knowledge, this is the first study with this approach in the genus Vriesea. Resumo Estudos sobre germinação e estabelecimento de plantas são peças-chave para entender o sucesso reprodutivo das plantas. Este trabalho teve como objetivo descrever a germinação in vitro e a mobilização de reservas na bromélia Vriesea friburgensis por meio de análises morfológicas, histoquímicas e bioquímicas. As condições utilizadas neste estudo para a germinação in vitro são adequadas. A partir do terceiro dia de inoculação in vitro, obteve-se germinação uniforme de 98%, apresentando alta qualidade fisiológica das sementes e alto potencial de produção de plântulas (94%). Há uma mobilização precoce de reservas, iniciada na fase de embebição. As reservas acumuladas no citoplasma do endosperma são degradadas por enzimas hidrolíticas fornecidas pela camada de aleurona. É possível que compostos nas paredes celulares do endosperma contribuam em menor grau na mobilização. Além disso, observou-se que o acúmulo de amido no cotilédone aumenta com a formação da plântula. Os resultados deste estudo fornecem informações para estudos futuros sobre ecologia, tecnologia de sementes e conservação desta espécie. Este estudo contribui para o conhecimento limitado da dinâmica das reservas durante a germinação e estabelecimento de plântulas em Bromeliaceae. Até onde sabemos, este é o primeiro estudo com esta abordagem no gênero Vriesea.

Published
2023

4. Parainfluenza-3 and bovine respiratory syncytial virus: intraherd correlation adjusted for sensitivity and specificity

Author

José Segura C., Daniel Figueroa Ch., Luis García-M., and Alfonso Pescador R.

Subjects
Bovine respiratory syncytial virus, design effect, intraclass correlation, parainfluenza-3 virus, Veterinary medicine, SF600-1100
Abstract

Objective. The purpose of this study was to compare the intra-class correlation coefficients (ICC) and design effects (D) estimates adjusted or unadjusted for sensibility (Se) and specificity (Sp) of the diagnostic tests using a Bayesian procedure. Materials and methods. Sera from 232 animals from 44 randomly selected herds, to detect antibodies against parainfluenza-3 virus (PIV3) from non-vaccinated dual-purpose cattle from Colima Mexico, were used. Only 176 animals from 33 herds were used to evaluate the presence of the bovine respiratory syncytial virus (BRSV). Results. The ICC and D values adjusted and unadjusted for PIV3 were 0.33, 2.73, 0.32, and 2.71, respectively. For BRSV the values were 0.31, 2.64, 0.28 and 2.49. Conclusions. The adjusted or unadjusted ICC and D estimates were similar because of the high Se and Sp of the diagnostic tests and the relatively high prevalence of the diseases here studied.

Published
2013

10. Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard

Author

Almeida V., Fraga H.P. de Freitas, Navarro B.B., Guerra M.P., and Pescador R.

Subjects
PLBs, micropropagation, epigenetics, orchids, in vitro culture
Abstract

Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.

Published
2017

14. Parainfluenza-3 and bovine respiratory syncytial virus: intraherd correlation adjusted for sensitivity and specificity

Author

Segura C, José, Figueroa Ch, Daniel, García-M, Luis, and Pescador R, Alfonso

Subjects
Bovine respiratory syncytial virus, virus parainfluenza-3, Correlación intraclase, efecto de diseño, design effect, parainfluenza-3 virus, intraclass correlation, virus respiratorio sincitial bovino
Abstract

Objective. The purpose of this study was to compare the intra-class correlation coefficients (ICC) and design effects (D) estimates adjusted or unadjusted for sensibility (Se) and specificity (Sp) of the diagnostic tests using a Bayesian procedure. Materials and methods. Sera from 232 animals from 44 randomly selected herds, to detect antibodies against parainfluenza-3 virus (PIV3) from non-vaccinated dual-purpose cattle from Colima Mexico, were used. Only 176 animals from 33 herds were used to evaluate the presence of the bovine respiratory syncytial virus (BRSV). Results. The ICC and D values adjusted and unadjusted for PIV3 were 0.33, 2.73, 0.32, and 2.71, respectively. For BRSV the values were 0.31, 2.64, 0.28 and 2.49. Conclusions. The adjusted or unadjusted ICC and D estimates were similar because of the high Se and Sp of the diagnostic tests and the relatively high prevalence of the diseases here studied. Objetivo. El propósito de este estudio fue comparar los valores de los coeficientes de correlación intraclase (ICC) y efecto de diseño (D) ajustados y no ajustados por la sensibilidad (Se) y especificidad (Es) de la prueba de diagnóstico usando procedimientos bayesianos. Materiales y métodos. Se utilizaron los sueros sanguíneos de 232 animales de 44 hatos seleccionados al azar, para detectar anticuerpos contra el virus de la parainfluenza-3 (VPI3) en bovinos de doble propósito, no vacunados en Colima, México. Se usaron 176 animales de 33 hatos para evaluar la presencia del virus respiratorio sincitial bovino (VRSB) Resultados. Los valores ajustados y no ajustados de ICC y D para VPI3 fueron 0.33, 2.73, 0.32 y 2.71, respectivamente. Para VRSB los valores fueron 0.31, 2.64, 0.28 y 2.49. Conclusiones. Los estimadores de ICC y D fueron similares debido a la alta Se y Es de las pruebas de diagnóstico y a la relativa alta prevalencia de las enfermedades estudiadas.

Published
2013

15. Alocação de recursos (carboidratos) no desenvolvimento inicial de plântulas de Schizolobium parahyba (Vell.) S. F. Blake (Fabaceae - Caesalpinioideae)

Author

WEIDLICH, E. W. A., PESCADOR, R. P., UHLMANN, A., EMANUELA WEHMUTH ALVES WEIDLICH, Pós graduação UFPR, ROSETE PESCADOR, UFSC, and ALEXANDRE UHLMANN, CNPF.

Subjects
Schizolobium Parahyba, Alocação de recurso, Plântula
Abstract

Utilizou-se Schizolobium parahyba, objetivando analisar a alocação dos teores de açúcares solúveis totais e de reserva (amido) e biomassas no desenvolvimento das plântulas. Após a quebra de dormência, 200 sementes foram semeadas em substrato composto por casca de arroz carbonizada e areia. Em cada coleta foram utilizadas 10 amostras para dosagem de açúcares solúveis totais e amido e 10 para mensuração das massas frescas e secas (MS e MF) no 7º, 14º, 21º, 28º, 35º e 42º dia após a germinação (DAG). As sementes apresentaram 94,5% de germinação. Os açúcares solúveis totais estão em maiores quantidades nas raízes, hipocótilo e epicótilos. Os cotilédones apresentaram maiores teores de amido, já que este é um órgão de reserva. O aumento da MF é diretamente proporcional ao incremento de açúcares solúveis e amido nas estruturas analisadas. As relações de biomassa e teores de açúcares solúveis totais e amido encontrados ressaltaram a mobilização dos compostos de reserva dos cotilédones nas outras partes da planta, à medida que a redução da biomassa e de açúcares dos cotilédones reflete o aumento na produção de biomassa de epicótilos, hipocótilos e raízes da plântula.

Published
2010

16. Enraizamento in vitro e aclimatização de mudas micropropagadas de Aloe vera L

Author

Silva, C. G., Debiasi, C. [UNESP], Pescador, R., FURB - Fundação Universidade Regional de Blumenau, and Universidade Estadual Paulista (Unesp)

Subjects
Indol-butyric acid, indolebutyric acid, in vitro study, micropropagation, pH, Acclimatization, greenhouse, sprout, plant growth, Substrate, temperature dependence, Aloe vera, photoperiodicity
Abstract

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:24Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:45:54Z : No. of bitstreams: 1 2-s2.0-34248154315.pdf: 304398 bytes, checksum: ef08ca3a7acd8a841dbd066dd331cdf7 (MD5) Made available in DSpace on 2014-05-27T11:22:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) With the objective to establish an efficient method of acclimatization of A. vera, the effect of the Indol-butíric acid (IBA) had been evaluated (T1=zero; T2=1.0 μmol L -1; T3=2.0 μmol L -1; T4=4.0 μmol L -1; T5=8.0 μmol L -1 and T2=10.0 μmol L -1) on in vitro rooting. For acclimatization the following substrates were tested: Ta=Carbonized rind of rice; Tb=Sand; Tc=Plantmax®; Td=Carbonized rind of rice + Sand in 1:1; Te=Carbonized rind of rice + Plantmax® in 1:1; e Tf=Sand + Plantmax® in 1:1 for the growth and development of the plantlets in greenhouse. One concluded that sprouts of A. vera, with average size of 1.5 cm must be taken in vitro rooting, during 30 days, in MS media culture supplemented with 1.0 μmol L -1 of IBA, sucrose (30 g L -1) and Agar (7 g L -1), pH adjusted for 5.8, kept in chamber of growth to a temperature of 25 ±2°C, with photoperiod of 16 hours light (50 μmol m -2 s -1 of intensity) and 8 hours dark. After that they must be acclimatized in substratum Plantmax®, during 60 days, in greenhouse (25 ±2°C, 80% of UR and 40% of reduction of the solar radiation). Of this, 95% of the A. vera plants will be ready to be transplanted in definitive for the production field, differentiated in that it says respect the genetic and phytosanitary quality. FURB - Fundação Universidade Regional de Blumenau, Blumenau-SC, 89010-971 CNPq UNESP - Universidade Estadual Paulista, Botucatu-SP, 18610-307 UNESP - Universidade Estadual Paulista, Botucatu-SP, 18610-307

Published
2007

19. Parainfluenza-3 and bovine respiratory syncytial virus: intraherd correlation adjusted for sensitivity and specificity

Author

Segura Correa, José C., Figueroa Ch, Daniel, García M, Luis, Pescador R, Alfonso, Segura Correa, José C., Figueroa Ch, Daniel, García M, Luis, and Pescador R, Alfonso

Abstract

Objective. The purpose of this study was to compare the intra-class correlation coefficients (ICC) and design effects (D) estimates adjusted or unadjusted for sensibility (Se) and specificity (Sp) of the diagnostic tests using a Bayesian procedure. Materials and methods. Sera from 232 animals from 44 randomly selected herds, to detect antibodies against parainfluenza-3 virus (PIV3) from non-vaccinated dual-purpose cattle from Colima Mexico, were used. Only 176 animals from 33 herds were used to evaluate the presence of the bovine respiratory syncytial virus (BRSV). Results. The ICC and D values adjusted and unadjusted for PIV3 were 0.33, 2.73, 0.32, and 2.71, respectively. For BRSV the values were 0.31, 2.64, 0.28 and 2.49. Conclusions. The adjusted or unadjusted ICC and D estimates were similar because of the high Se and Sp of the diagnostic tests and the relatively high prevalence of the diseases here studied., Objetivo. El propósito de este estudio fue comparar los valores de los coeficientes de correlación intraclase (ICC) y efecto de diseño (D) ajustados y no ajustados por la sensibilidad (Se) y especificidad (Es) de la prueba de diagnóstico usando procedimientos bayesianos. Materiales y métodos. Se utilizaron los sueros sanguíneos de 232 animales de 44 hatos seleccionados al azar, para detectar anticuerpos contra el virus de la parainfluenza-3 (VPI3) en bovinos de doble propósito, no vacunados en Colima, México. Se usaron 176 animales de 33 hatos para evaluar la presencia del virus respiratorio sincitial bovino (VRSB) Resultados. Los valores ajustados y no ajustados de ICC y D para VPI3 fueron 0.33, 2.73, 0.32 y 2.71, respectivamente. Para VRSB los valores fueron 0.31, 2.64, 0.28 y 2.49. Conclusiones. Los estimadores de ICC y D fueron similares debido a la alta Se y Es de las pruebas de diagnóstico y a la relativa alta prevalencia de las enfermedades estudiadas.

Published
2013

21. Effect of human immunodeficiency virus infection on hepatitis C virus infection in hemophiliacs

Author

From the Section of Gastroenterology and Hepatology, and Hematology and Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana; Chiron Corporation, Emeryville, California; Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Box 0362, 48109, Ann Arbor, Michigan, From the Section of Gastroenterology and Hepatology, and Hematology and Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana; Chiron Corporation, Emeryville, California, Ann Arbor, Sanchez-Pescador, R., Leissinger, C., Lagier, R., Lok, A.S.F., Ghany, Marc G., From the Section of Gastroenterology and Hepatology, and Hematology and Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana; Chiron Corporation, Emeryville, California; Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Box 0362, 48109, Ann Arbor, Michigan, From the Section of Gastroenterology and Hepatology, and Hematology and Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana; Chiron Corporation, Emeryville, California, Ann Arbor, Sanchez-Pescador, R., Leissinger, C., Lagier, R., Lok, A.S.F., and Ghany, Marc G.

Abstract

Chronic liver disease due to hepatitis C virus (HCV) infection is a major problem in hemophiliacs. Recent reports suggested that hemophiliacs coinfected with hepatitis C virus and human immunodeficiency virus (HIV) have an increased incidence of liver failure but the mechanism of accelerated liver injury is not clear. We tested plasma from 100 hemophiliacs for anti-HCV by second generation ELISA, anti-HIV by EIA, and HCV RNA and HIV RNA by branched DNA and polymerase chain reaction assays to determine if hemophiliacs coinfected with HCV and HIV have higher HCV RNA levels and more active liver disease. Seventy-nine (79%) patients were anti-HCV positive, of whom 85% were HCV RNA positive. None of the anti-HCV-negative patients had detectable HCV RNA in plasma. Forty-two (42%) patients were anti-HIV positive, of whom 47% had detectable HIV RNA. All the anti-HIV-positive patients were also anti-HCV positive. The prevalence of both anti-HCV and anti-HIV increased significantly with age. There was no difference in HCV RNA levels between anti-HIV-positive and anti-HIV-negative patients (mean: 21??4 vs 18??5 Meq/ml), although HCV RNA levels were significantly higher in anti-HIV-positive patients with CD4 counts<200/mm 3 (P =0.008). There was an inverse correlation between HCV RNA levels and CD4 counts but no correlation was found between HCV RNA and serum aminotransferase levels. We found a high prevalence of HCV and HIV coinfection in our hemophiliacs. Hepatitis C virus replication appears to be increased in patients with severe immunodeficiency secondary to progressive HIV infection. However, there was no correlation between HCV RNA and serum ALT level, suggesting that HCV is not directly cytopathic.

Published
2006

24. Induction and development of nodular cluster cultures in Vriesea reitzii (Leme and Costa), an endangered bromeliad from the Brazilian Atlantic Forest.

Author

DAL VESCO, L. L., VIEIRA, P. M., CORREDOR, J. P., PESCADOR, R., WELTER, L. J., and GUERRA, M. P.

Subjects
BROMELIACEAE, ENDANGERED plants, PLANT regulators, PLANT growing media, PLANT morphology
Abstract

The present work studied the induction, multiplication and development of nodular cluster cultures (NCC) of Vriesea reitzii, an endangered bromeliad species native to the Brazilian Atlantic Forest Biome, in different culture media under different culture conditions. Leaf basal segments of in vitrro-grown plantlets were used as explants and inoculated into Murashige and Skoog (MS) liquid medium supplemented with different types and concentrations of plant growth regulators (PGRs) in the presence or absence of light, followed by sub-culturing in or on PGR-free liquid or solidified culture media to promote multiplication. The induction of NCC, which occurred from the basal region of the explants, was observed in all culture media tested. Morphological features and regenerative efficiency were ascribed to the different culture media, based mainly on medium type, and the concentration and combination of PGRs, as well as on exposure to light or dark conditions. Green-coloured NCC with a granular texture showed a high regenerative efficiency in response to MS culture medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) plus 2 µM N6(2-isopentenyl) adenine (2-iP), or plus 2 µM 6-benzylaminopurine (BAP), in cultures incubated under a 16 h photoperiod. Throughout the multiplication stage, regenerative efficiency showed a direct correlation with the composition of the induction medium in which the NCC had originated. Thus, NCC that originated from MS culture medium supplemented with 4 µM NAA and 2 µM 2-iP showed the highest regenerative efficiency (7.2 g g-1 of NCC). NCC that originated from MS culture medium supplemented with 2 µM NAA plus 4 µM BAP showed the highest number of regenerated microshoots (140.0 microshoots g-1). Therefore, the competence of NCC for having a high regenerative efficiency was related to the culture environment and to the type, concentration, and combination of PGRs added to the basal MS culture medium. In vitro-shoot elongation was obtained on filter paper bridges over PGR-free basal MS (BMS) liquid medium. Fully-developed and acclimatised plants were obtained in an ex vitro environment. Taking together, the results of the present study provide new and useful information on an in vitro protocol for the endangered bromeliad, V. reitzii, based on regeneration from nodular cluster cultures. [ABSTRACT FROM AUTHOR]

Published
2014
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25. MORFO-HISTOLOGIA DE CULTURAS NODULARES NA MICROPROPAGAÇÃO DE Aechmea setigera MART. EX SCHULT. & SCHULT. F. (BROMELIACEAE).

Author

Fermino Júnior, P. C. P., Lando, A. P., Santos, M., and Pescador, R.

Abstract

Copyright of Evidência is the property of Evidencia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2014

34. Dynamics of free and 3H-labelled glutamine concentrations during zygotic and somatic embryogenesis of Feijoa [Acca sellowiana (0. Berg.) Burret].

Author

PESCADOR, R., KERBAUY, G. B., FRAGA, H. P. F., HAMASAKI, R. M., TAVARES, L. B. B., and GUERRA, M. P.

Subjects
GLUTAMINE, PLANT morphogenesis, SOMATIC embryogenesis, AMINO acids, CELL culture, TISSUE culture
Abstract

Amino acids play fundamental roles in plant morphogenesis. Among sources of organic nitrogen (N), glutamine has frequently been used during the establishment and maintenance of cell and tissue cultures. The aim of this study was analyse endogenous levels of glutamine during somatic and zygotic embryogenesis of Acca sellowiana (Feijoa or pineapple guava). The in vitro absorption of ³H-labelled glutamine was investigated. Zygotic embryos and embryogenic cultures (EC) were evaluated at 30 d and 70 d after explant inoculation onto the medium. Endogenous levels of glutamine were similar during zygotic and somatic embryogenesis, and showed a gradual decline until day-24 in culture. The highest rates of ³H-labelled glutamine uptake were observed during the first 2 h of incubation, resulting in values of 6.29 µmol g-1 fresh weight (FW) for zygotic embryos, 14.43 µmol g-1 FW for EC after 30 d, and 13.85 µmol g-1 FW for EC after 70 d. These results showed that the decreased levels of glutamine observed during the initial phase of development may be related to de novo protein synthesis and mobilisation during embryo maturation. The absorption of glutamine in the first 2 h of incubation also emphasises its involvement as an important source of N during morphogenesis of somatic and zygotic embryos. [ABSTRACT FROM AUTHOR]

Published
2012
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40. Parâmetros fisiológicos em videiras ‘Paulsen 1103’ (Vitis berlandieri x Vitis rupestris) inoculadas com fungos micorrízicos arbusculares em solo contaminado com cobre

Author

Rosa Daniel José, Ambrosini Vítor Gabriel, Brunetto Gustavo, Soares Cláudio Roberto Fonsêca Sousa, Borghezan Marcelo, and Pescador Rosete

Subjects
metal pesado, micorrizas arbusculares, assimilação de co2, vitis sp., Plant culture, SB1-1110
Abstract

A aplicação de fungicidas foliares em vinhedos incrementa o teor de cobre (Cu) no solo, que pode modificar parâmetros fisiológicos de videiras jovens transplantadas em solos de vinhedos erradicados. A inoculação com fungos micorrízicos arbusculares (FMA) pode reduzir a disponibilidade de Cu às videiras e, com isso, reduzir o efeito tóxico do Cu nas células de plantas jovens. O trabalho objetivou avaliar alterações fisiológicas no porta-enxerto de videira ‘P1103’ (Vitis berlandieri x Vitis rupestris) cultivado em solo com alto teor de Cu e inoculado com espécies de FMA. O experimento foi conduzido em casa de vegetação. As videiras foram acondicionadas em vasos. No momento do transplante, as raízes foram inoculadas com os FMA: Dentiscutata heterogama, Acaulospora morrowiae e Rhizophagus clarus, além de um tratamento controle, não-inoculado. O experimento foi conduzido em blocos ao acaso com sete repetições. O cultivo foi realizado durante 76 dias, ao final do qual foi avaliado a altura de planta, a área foliar, as taxas de trocas gasosas, colonização micorrízica, matéria seca de raízes, caule e folha; número de folhas, diâmetro de colmo, teores de carboidratos solúveis totais, amido total, clorofila a, b e total, carotenoides, teores de Cu e P nas plantas. Dentre os FMA avaliados, o R. clarus promoveu maior benefício para o crescimento das plantas e reduziu o teor de Cu nos tecidos. As maiores taxas de assimilação de CO2 e condutância estomática ocorreram nas plantas inoculadas com D. heterogama. Os FMAs estudados amenizaram a toxicidade do cobre às videiras jovens.

Published
2016
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48. Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.

Author

Bell, G I, Gerhard, D S, Fong, N M, Sanchez-Pescador, R, and Rall, L B

Abstract

Overlapping recombinant clones that encompass the insulin-like growth factor (IGF) I and II genes have been isolated from a human genomic DNA library. Each gene is present once per haploid genome; the IGF-I gene spans greater than 35 kilobase pairs (kbp) and the IGF-II gene is at least 15 kbp. The exon-intron organization of these genes is similar, each having four exons, which is one more than the related insulin gene. Comparison of the restriction endonuclease cleavage maps of the IGF-II and insulin genes, including their flanking regions and hybridization with an IGF-II cDNA probe, revealed that they are adjacent to one another. The IGF-II and insulin genes have the same polarity and are separated by 12.6 kbp of intergenic DNA that includes a dispersed middle repetitive Alu sequence. The order of the genes is 5'-insulin-IGF-II-3'.

Published
1985
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49. Tight linkage of genes that encode the two glutamate synthase subunits of Escherichia coli K-12

Author

Lozoya, E, Sanchez-Pescador, R, Covarrubias, A, Vichido, I, and Bolivar, F

Abstract

A hybrid deoxyribonucleic acid molecule, plasmid pRSP20, which was isolated from the Clarke and Carbon Escherichia coli gene bank, was shown to complement the gltB31 mutation, which affects the synthesis of glutamate synthase in E. coli strain PA340. We present evidence which demonstrates that plasmid pRSP20 carries an 8-megadalton E. coli chromosomal fragment, including the genes encoding the two unequal glutamate synthase subunits. Polypeptides with molecular weights of about 135,000 and 53,000, which comigrated with purified E. coli glutamate synthase subunit polypeptides and immunoprecipitated with antibodies to E. coli glutamate synthase, were synthesized by minicells carrying the pRSP20 plasmid.

Published
1980
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50. Defibrotide Affects the Anticoagulant and Lipase-Releasing Activities of Heparin

Author

Porta, R., Pescador, R., Mantovani, M., and Prino, G.

Abstract

Defibrotide (DEF), an antithrombotic drug with no anticoagulant activity, given concomitantly with heparin potentiates its effects on thrombin time and lipase-releasing activity. Unlike heparin, DEF has hardly any lipase-releasing activity of its own. Most likely DEF interferes with pharmacokinetics of heparin. It is clear from the data reported here that when used in combination with DEF heparin should be administered at low doses and the coagulation parameters carefully followed.

Published
1990
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