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24. Analyses of monoclonal antibodies reacting with porcine wCD6 Results from the Second International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Petrinec, N., and Saalmüller, A.

Subjects
Antigens, Differentiation, T-Lymphocyte, General Veterinary, Swine, T-cells, Immunology, Antibodies, Monoclonal, Flow Cytometry, Antigen-Antibody Reactions, Porcine wCD6, Antigens, CD, T-Lymphocyte Subsets, Monoclonal antibodies, porcine wCD6, Animals
Abstract

Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated from activated T-cells a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, reacts with a separate epitope on porcine wCD6.

Published
1998

27. Overview of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Author

Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., Zwart, R. J., Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., and Zwart, R. J.

Abstract

The aim of the Third International Workshop on Swine Leukocyte Differentiation Antigens (CD workshop), supported by the Veterinary Immunology Committee (VIC) of the International Union of Immunological Societies (IUIS), was to standardize the assignment of monoclonal antibodies (mAb) reactive with porcine leukocyte differentiation antigens and to define new antibody clusters, using nomenclature in accordance with human and ruminant CD nomenclature, as agreed at the summary meeting of the Second International Swine CD Workshop in Davis, 1995 only mAb with proven reactivity for the orthologous porcine gene product or cross-reactivity for the human gene products, were given the full CD nomenclature, all other allocations were prefixed with 'w'.As in previous workshops, the overall organization was entrusted to the chair and first author, with support by the chair of the previous workshop and second author.In addition to the existing 26 pig leukocyte CD/SWC determinants established in previous workshops, this workshop established/confirmed another 11 CDs for pig leukocytes, identified by a total of 21 mAb CD11R1 (2 mAb), CD11R2 (1 mAb), CD11R3 (4 mAb), wCD40 (1 mAb), wCD46 (4 mAb), wCD47 (3 mAb), wCD49d (1 mAb), CD61 (1 mAb), wCD92 (1 mAb), wCD93 (1 mAb) and CD163 (2 mAb). Copyright © 2001 Elsevier Science B.V.

Published
2001

33. Analyses of monoclonal antibodies reacting with porcine CD5:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Tomaskovic, M., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Saalmüller, A., Aasted, B., Pescovitz, M. D., Book, B. K., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Tomaskovic, M., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Saalmüller, A., and Aasted, B.

Published
1998

34. Report on the analyses of mAb reactive with porcine CD8 for the 2. International Swine CD Workshop

Author

Zuckermann, F. A., Pescovitz, M. D., Aasted, B., Dominguez, J., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W. C., Barbosa, I. R., Saalmüller, A., Zuckermann, F. A., Pescovitz, M. D., Aasted, B., Dominguez, J., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W. C., Barbosa, I. R., and Saalmüller, A.

Published
1998

35. Analyses of monoclonal antibodies reacting with porcine wCD6:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Petrinec, N., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Petrinec, N., and Saalmüller, A.

Published
1998

36. Summary of the first round analyses of the 2. International Swine CD Workshop

Author

Saalmüller, A., Pauly, T., Aasted, B., Jensen, K. T., Sachs, D. H., Nielsen, J., Arn, S., Davis, W. C., Park, Y. H., McCullough, K., Summerfield, A., Murtaugh, M., Pampusch, M. S., Burger, K. D., Laber, J., Pescovitz, M. D., Stokes, C. R., Haverson, K., Boyd, P. C., Lunney, J. K., Saalmüller, A., Pauly, T., Aasted, B., Jensen, K. T., Sachs, D. H., Nielsen, J., Arn, S., Davis, W. C., Park, Y. H., McCullough, K., Summerfield, A., Murtaugh, M., Pampusch, M. S., Burger, K. D., Laber, J., Pescovitz, M. D., Stokes, C. R., Haverson, K., Boyd, P. C., and Lunney, J. K.

Published
1998

37. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., and Saalmüller, A.

Published
1998

38. Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens

Author

Saalmüller, A., Pauly, T., Lunney, J. K., Boyd, P. C., Aasted, B., Sachs, D. H., Arn, S., Bianchi, A., Binns, R. M., Licence, S., Whyte, A., Blecha, A., Chen, Z., Chu, R. M., Davis, W. C., Denham, S., Yang, H., Whittall, T., Parkhouse, R. M. E., Dominguez, J., Ezquerra, A., Alonso, F., Horstick, G., Howard, C., Sopp, P., Kim, Y. B., Lipp, J., Mackay, C., Magyar, A., McCullough, K., Arriens, A., Summerfield, A., Murtaugh, M., Nielsen, J., Novikov, B., Pescovitz, M. D., Schuberth, H. J., Leibold, W., Schütt, C., Saalmüller, A., Pauly, T., Lunney, J. K., Boyd, P. C., Aasted, B., Sachs, D. H., Arn, S., Bianchi, A., Binns, R. M., Licence, S., Whyte, A., Blecha, A., Chen, Z., Chu, R. M., Davis, W. C., Denham, S., Yang, H., Whittall, T., Parkhouse, R. M. E., Dominguez, J., Ezquerra, A., Alonso, F., Horstick, G., Howard, C., Sopp, P., Kim, Y. B., Lipp, J., Mackay, C., Magyar, A., McCullough, K., Arriens, A., Summerfield, A., Murtaugh, M., Nielsen, J., Novikov, B., Pescovitz, M. D., Schuberth, H. J., Leibold, W., and Schütt, C.

Published
1998

39. Report on the analyses of mAb reactive with porcine CD8 for the Second International Swine CD Workshop

Author

Lee, Rogan [0000-0001-8364-9515], Zuckermann, F., Pescovitz, M. D., Aasted, B., Domínguez, Javier, Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Saalmüller, A., Lee, Rogan [0000-0001-8364-9515], Zuckermann, F., Pescovitz, M. D., Aasted, B., Domínguez, Javier, Trebichavsky, I., Novikov, B., Valpotic, I., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., and Saalmüller, A.

Abstract

Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, 090; UCP1H12-2, 139, and PG164A, 051) bind exclusively to CD8+ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 3-3 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8+ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8(low) and CD8(high) cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8(low) and CD8(high) lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4-/CD8(high) lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, 094; and SwNL554.1, 009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8+

Published
1998

40. Analyses of monoclonal antibodies reacting with porcine CD5 Results from the Second International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, Jordy, Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Tomaskovic, M., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Richard, Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, Jordy, Bullido, R., Trebichavsky, I., Novikov, B., Valpotic, I., Tomaskovic, M., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Richard, and Saalmüller, A.

Abstract

Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAbs fell within cluster T13 including the CD5a standard b53b7 (No. 174). The two new mAbs 1H6/8 (No. 058) and BB6-9G12 (No. 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAbs b53b7 to peripheral lymphocytes. These mAbs, therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAbs to eight, all of which appear to recognize the same epitope.

Published
1998

41. Summary of workshop findings for antibodies reacting with porcine T- cells and activation antigens Results from the Second International Swine CD Workshop

Author

Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., Saalmüller, A., Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., and Saalmüller, A.

Abstract

After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assigments included two CD2; one CD4; two CD5; one 7 CD and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.

Published
1998

42. Overview of the Second International Workshop to define Swine Cluster of Differentiation (CD) antigens

Author

Lee, Rogan [0000-0001-8364-9515], Mackay, Charles R. [0000-0002-6338-7340], Saalmüller, A., Pauly, T., Lunney, J. K., Boyd, P. C., Aasted, B., Sachs, David H., Arn, S., Bianchi, A. T. J., Binns, R. M., Licence, S., Whyte, A., Blecha, F., Chen, Zhang, Chu, R. M., Davis, William, Denham, S., Yang, Huaizhi, Whittall, T., Parkhouse, R. Michael, Domínguez, Javier, Ezquerra Martínez, Ángel, Alonso, Fernando, Horstick, G., Howard, C., Sopp, P., Kim, Yoon Berm, Lipp, J., Mackay, Charles R., Magyar, A., McCullough, Kenneth C., Arriens, A., Summerfield, A., Murtaugh, M., Nielsen, Jens, Novikov, B., Pescovitz, M. D., Schuberth, H. J., Leibold, W., Schütt, C., Shimizu, M., Stokes, Christopher, Haverson, K., Bailey, M., Tlaskalova, H., Trebichavsky, I., Valpotic, I., Walker, J., Lee, Rogan, Zuckermann, F., Lee, Rogan [0000-0001-8364-9515], Mackay, Charles R. [0000-0002-6338-7340], Saalmüller, A., Pauly, T., Lunney, J. K., Boyd, P. C., Aasted, B., Sachs, David H., Arn, S., Bianchi, A. T. J., Binns, R. M., Licence, S., Whyte, A., Blecha, F., Chen, Zhang, Chu, R. M., Davis, William, Denham, S., Yang, Huaizhi, Whittall, T., Parkhouse, R. Michael, Domínguez, Javier, Ezquerra Martínez, Ángel, Alonso, Fernando, Horstick, G., Howard, C., Sopp, P., Kim, Yoon Berm, Lipp, J., Mackay, Charles R., Magyar, A., McCullough, Kenneth C., Arriens, A., Summerfield, A., Murtaugh, M., Nielsen, Jens, Novikov, B., Pescovitz, M. D., Schuberth, H. J., Leibold, W., Schütt, C., Shimizu, M., Stokes, Christopher, Haverson, K., Bailey, M., Tlaskalova, H., Trebichavsky, I., Valpotic, I., Walker, J., Lee, Rogan, and Zuckermann, F.

Abstract

The aim of the Second International Swine. Cluster of Differentiation (CD) Workshop, supported by the Veterinary Immunology Committee (VIC) of the International Union of Immunological Societies (IUIS), was to standardize the assignment of monoclonal antibodies (mAb) reactive with porcine leukocyte differentation antigens and to define new antibody clusters. At the summary meeting of the workshop in July, 1995, revisions in the existing nomenclature for Swine CD were approved, so that the rules are now in accord with those for human and ruminant CD. Swine CD numbers will now be given to clusters of mAb to swine orthologues of human CD molecules when homology is proven by (1) suitable tissue distribution and lymphoid cell subset expression, (2) appropriate molecular mass of the antigen recognized by the mAbs, and (3) reactivity of mAbs with the cloned swine gene products, or cross-reactivity of the mAb on the human gene products. In some cases, this reactivity would not be fully proven, mainly due to the lack of cloned gene products; for these CD antigens, the respective clusters will be assigned by the prefix 'w' which will lead to 'wCD' antigens. As a result of the Second International Swine CD Workshop the assignment of 16 mAb to existing CD groups (CD2a, CD4a, CD5a, wCD6, wCD8, CD14, CD18a, wCD21, wCD25) was confirmed, and 2 mAb to existing swine workshop clusters (SWC). More importantly, for the work on the porcine immune system, was the definition of 5 new swine CD antigens, namely CD3 (recognized by 6 new mAb and 3 epitopes), CD16 (1 new mAb), wCD29 (2 mAb), CD45RA (3 mAb) and CD45RC (1 new mAb). Finally, the demarcation of two new SWC molecules in swine, SWC8 (2 mAb) and SWC9 (2 mAb) was confirmed.

Published
1998

43. The Second International Swine CD Workshop

Author

Saalmüller, A., Denham, S., Haverson, K., Davis, B., Domínguez, Javier, Pescovitz, M. D., Stokes, Christopher, Zuckermann, F., Lunney, J. K., Saalmüller, A., Denham, S., Haverson, K., Davis, B., Domínguez, Javier, Pescovitz, M. D., Stokes, Christopher, Zuckermann, F., and Lunney, J. K.

Published
1996

44. Analysis of monoclonal antibodies reactive with the porcine CD2 antigen

Author

Pescovitz, M. D., Aasted, B., Canals, A., Domínguez, Javier, Sánchez-Vizcaíno, J. M., Pospisil, R., Sinkora, J., Salmon, H., Valpotic, I., Davis, William, Arn, S., Sachs, David H., Lunney, J. K., Zuckermann, F., Weiland, E., Saalmüller, A., Pescovitz, M. D., Aasted, B., Canals, A., Domínguez, Javier, Sánchez-Vizcaíno, J. M., Pospisil, R., Sinkora, J., Salmon, H., Valpotic, I., Davis, William, Arn, S., Sachs, David H., Lunney, J. K., Zuckermann, F., Weiland, E., and Saalmüller, A.

Abstract

As result of the First International Swine CD Workshop, six monoclonal antibodies (mAbs) (numbers 014, 023, 024, 057, 128, and 130) clustered closely to the internal standard anti-porcine CD2 mAb, MSA4. Despite the close clustering, the cluster was split into two subgroups. To further characterize the relationship between these mAbs, they were used in flow cytometry to inhibit binding of MSA4 to porcine lymphocytes. mAbs 014 (1038-8-31), 023 (MAC83), 024 (MAC80), 057 (PG168), and 128 (MSA4) completely inhibited the binding of MSA4, mAb 130 (MSA2) failed to inhibit MSA4 binding. On dual parameter flow cytometry comparing MSA2 with MSA4, all MSA2+ cells were MSA4+, two thirds of the MSA4+ cells were MSA2-. We conclude that five of the mAbs bind to the same or a closely related epitope on porcine lymphocytes. mAb 130 appears to have aberrantly clustered with the CD2 group of mAb. © 1994.

Published
1994

45. Analyses of monoclonal antibodies reactive with porcine CD6

Author

Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pauly, T., Pescovitz, M. D., Pospisil, R., Salmon, H., Trebichavsky, I., Valpotic, I., Sánchez-Vizcaíno, J. M., Weiland, E., Zuckermann, F., Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pauly, T., Pescovitz, M. D., Pospisil, R., Salmon, H., Trebichavsky, I., Valpotic, I., Sánchez-Vizcaíno, J. M., Weiland, E., and Zuckermann, F.

Abstract

Amongst the monoclonal antibodies (mAbs) submitted to the first porcine CD workshop, two mAbs (workshop numbers 055 and 120) could be identified to recognize the porcine CD6 analogue. Both mAbs seemed to be highly T-cell specific and showed neither reactivity with cells of the myeloic lineage nor with B lymphocytes. The observed molecular mass of the antigen precipitated by mAb 120 of 110 kDa confirmed this classification. Without molecular analyses of the antigen recognized by mAb 055, but similar staining pattern in FCM compared with 120, mAb 055 was allocated to the wCD6 subcluster. © 1994.

Published
1994

46. Analysis of mAb reactive with the porcine SWC1

Author

Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Pauly, T., Pescovitz, M. D., Pospisil, R., Salmon, H., Sinkora, J., Summerfield, A., Valpotic, I., Sánchez-Vizcaíno, J. M., Zuckermann, F., Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Pauly, T., Pescovitz, M. D., Pospisil, R., Salmon, H., Sinkora, J., Summerfield, A., Valpotic, I., Sánchez-Vizcaíno, J. M., and Zuckermann, F.

Abstract

Among 54 mAb determined to be reactive with porcine T lymphocytes and/or activation antigens, eight mAb (workshop Nos. 005, 031, 080, 091, 092, 093, 094 and 110) derived from different laboratories grouped together in the T11 cluster and were ordered into the SWC1. One mAb (No. 111) which belong also to this group was lost during the workshop. The SWC1 antigen is a molecule expressed on the majority of leukocytes, resting T lymphocytes, monocytes and granulocytes, but not on B lymphocytes. On T lymphocytes it is down-regulated after activation. The molecular mass of the antigen is unknown. Epitope analyses revealed that seven out of the nine mAb recognized similar epitopes on the SWC1 molecule. © 1994.

Published
1994

47. Analyses of mAb reactive with porcine CD8

Author

Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pescovitz, M. D., Pospisil, R., Salmon, H., Trebichavsky, I., Valpotic, I., Sánchez-Vizcaíno, J. M., Zuckermann, F., Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pescovitz, M. D., Pospisil, R., Salmon, H., Trebichavsky, I., Valpotic, I., Sánchez-Vizcaíno, J. M., and Zuckermann, F.

Abstract

Among all mAb submitted to the first porcine CD workshop, based on FCM analyses six mAb could be identified to recognize the porcine CD8 analogue (workshop Nos. 004, 051, 052, 053, 108 and 109). In immunoprecipitation studies three mAb (Nos. 004, 108 and 109) recognized an antigen with an apparent molecular mass of about 35 kDa under reducing conditions and about 70 kDa under non-reducing conditions. The molecular masses of the antigens recognized by the three other mAb (Nos. 051, 052 and 053) are still unknown. Epitope analyses performed by blocking experiments led to the determination of two CD8 epitopes CD8a and CD8b. CD8a is recognized by mAb Nos. 004, 051 and 052, and CD8b by Nos. 053, 108 and 109. © 1994.

Published
1994

48. Summary of workshop findings for porcine T-lymphocyte antigens

Author

Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pescovitz, M. D., Pospisil, R., Salmon, H., Tlaskalova, H., Valpotic, I., Sánchez-Vizcaíno, J. M., Weiland, E., Zuckermann, F., Saalmüller, A., Aasted, B., Canals, A., Domínguez, Javier, Goldman, T., Lunney, J. K., Maurer, S., Pescovitz, M. D., Pospisil, R., Salmon, H., Tlaskalova, H., Valpotic, I., Sánchez-Vizcaíno, J. M., Weiland, E., and Zuckermann, F.

Abstract

Fifty-four mAb preselected in the first round of the first porcine CD workshop for their possible reactivity with T-lymphocyte specific antigens and/or activation antigens were further analysed in a second round. PBMC, thymocytes and nylon-wool purified T lymphocytes derived from peripheral blood, mesenteric lymph nodes and spleen served as target cells for flow cytometric analyses. For the classification of activation antigens several experiments were performed with activated, mitogen-stimulated T lymphocytes and long-term T-lymphocyte cultures. Out of the 54 mAb, 35 mAb could be distributed to six different CD clusters and two swine workshop clusters (SWC). Five mAb could be distributed to the porcine CD2, four mAb to the CD4. Six mAb seemed to recognize the porcine CD5 and two mAb the porcine CD6 analogue. Six mAb were directed against the porcine CD8, whereas two different epitopes could be defined. One mAb was directed against the porcine CD25 analogue. Nine mAb could be clustered to the SWC1, defining an antigen on T lymphocytes and cells of the myeloic linage. Two mAb with high T-cell specificity were clustered to the SWC2. © 1994.

Published
1994

49. Overview of the first international workshop to define swine leukocyte cluster of differentiation (CD) antigens

Author

Mackay, Charles R. [0000-0002-6338-7340], Lunney, J. K., Walker, Karen, Goldman, T., Aasted, B., Bianchi, A. T. J., Binns, R. M., Licence, S., Bischof, R., Brandon, M., Blecha, F., Kielian, T. L., McVey, D. S., Chu, R. M., Carr, M., Howard, C., Sopp, P., Davis, William, Dvorak, Pavel, Domínguez, Javier, Canals, A., Sánchez-Vizcaíno, J. M., Kim, Yoon Berm, Laude, H., Mackay, Charles R., Magnusson, U., McCullough, Kenneth C., Misfeldt, M., Murtaugh, M., Molitor, T., Choi, C., Pabst, R., Parkhouse, R. Michael, Denham, S., Yang, Huaizhi, Pescovitz, M. D., Pospisil, R., Tlaskalova, H., Saalmüller, A., Weiland, E., Salmon, H., Sachs, David H., Arn, S., Shimizu, M., Stokes, Christopher, Stevens, K., Valpotic, I., Zuckermann, F., Husmann, R., Mackay, Charles R. [0000-0002-6338-7340], Lunney, J. K., Walker, Karen, Goldman, T., Aasted, B., Bianchi, A. T. J., Binns, R. M., Licence, S., Bischof, R., Brandon, M., Blecha, F., Kielian, T. L., McVey, D. S., Chu, R. M., Carr, M., Howard, C., Sopp, P., Davis, William, Dvorak, Pavel, Domínguez, Javier, Canals, A., Sánchez-Vizcaíno, J. M., Kim, Yoon Berm, Laude, H., Mackay, Charles R., Magnusson, U., McCullough, Kenneth C., Misfeldt, M., Murtaugh, M., Molitor, T., Choi, C., Pabst, R., Parkhouse, R. Michael, Denham, S., Yang, Huaizhi, Pescovitz, M. D., Pospisil, R., Tlaskalova, H., Saalmüller, A., Weiland, E., Salmon, H., Sachs, David H., Arn, S., Shimizu, M., Stokes, Christopher, Stevens, K., Valpotic, I., Zuckermann, F., and Husmann, R.

Published
1994

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