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Um chromosomale Veränderungen zu untersuchen, die mit dem metastatischen Phänotyp assoziiert sind, zu identifizieren, wurde die Comparative Genomische Hybridisierung (CGH) an Plattenepithelkarzinomen der Lunge durchgeführt. Der statistische Vergleich zwischen metastasierten und nicht-metastasierten Tumoren legte nahe, daß Deletionen von 3p12, 4p16, 6p22-p24, 6q24-q26, 8p21-p23, 10q und 21q22 sowie DNA-Gewinne bei 1q21-q25, 8q, 9q34, 11q13 und 15q11-q13 signifikant mit der Metastasierung assoziiert waren. Ausgedehnte Allelotypisierungsstudien konnten 3 minimale Deletionsregionen auf Chromosom 10q nachweisen. Die Untersuchung der Kandidatengene PTEN/MMAC1, MXI1 und DMBT1 in diesen Bereichen war jedoch negativ. Zur Identifizierung neuer Kandidatengene in der Lungenkrebs-Progression zu identifizieren, wurde die Subtraktive Suppressions-Hybridisierung (SSH) eingesetzt. Der Expressionsvergleich einer metastasierten Adenokarzinomzellinie mit einer Primärkultur normaler Lungenepithelzellen resultierte in der Klonierung von 2 cDNA-Bibliotheken mit insgesamt 752 Klonen, welche sequenziert und im Northern Blot auf differentielle Expression untersucht wurden. In weniger als 40% zeigten sich Homologien zu bereits bekannten Genen. Die weitere Analyse eines unbekannten cDNA-Fragmentes führte zur Klonierung und Charakterisierung eines neuen Genes, welches das humane Calcyclin-bindende Protein kodiert, auf Chromosom 1q24-q25. Die Überexpression dieses Genes in Lungenkarzinomzellinien sowie die Aufdeckung intra- und interchromosomaler Rearrangements mittels Fluoreszenz- in situ-Hybridisierung (FISH) weist auf eine mögliche Bedeutung in der Progression von Lungenkarzinomen hin. To identify chromosomal imbalances associated with the metastatic phenotype Comparative Genomic Hybridization (CGH) was applied to squamous cell carcinomas of the lung. The comparison of metastatic and non-metastatic tumors suggested that deletions of 3p12, 4p16, 6p22-p24, 6q24-q26, 8p21-p23, 10q, and 21q22 as well as DNA gains of 1q21-q25, 8q, 9q34, 11q13, and 15q11-q13 were significant associated with the metastasis formation. Extensive allelotyping studies revealed 3 minimal deleted regions of chromosome 10q. The mutational analysis of the three candidate genes PTEN/MMAC1, MXI1, and DMBT1 located near these regions was negative. To isolate new candidate genes involved in lung cancer progression suppression subtractive hybridization (SSH) was used. The comparison of the expression pattern of a metastatic lung adeno carcinoma cell line with a primary culture of normal airway epithelial cells resulted in the cloning of 752 cDNA fragments which were sequenced and investigated by Northern blot hybridization. The sequence comparison showed homology to known genes in less than 40%. The further investigation led to the cloning and characterization of a new gene encoding the human Calcyclin-binding protein on chromosome 1q24-q25. The gene was overexpressed in a panel of lung cancer cell lines and showed intra- and interchromosomal reaarangements using fluorescence in situ- hybridization (FISH) suggesting the involvement of this gene in lung cancer progression.

Treball desenvolupat dins el marc del programa 'European Project Semester'. This project, TESEUS, is being carried out within the umbrella of the ongoing Urban Node study and focuses on the development of a computational system capable of handling a huge amount of data received from urban nodes in a smart city. There are already existing computer systems with computational power capable of handling the amount of data a smart city requires. Although these computers may provide all the power required to maintain and manage a smart city, they can always be made more efficient. The lack of efficiency comes from the inability to perform in a scalable way. A computer is not always required to work with its full power. The way supercomputers are designed is rather inefficient when managing lesser amounts of data than expected. The Teseus project objective is the possibility of building a low cost, scalable data server with focus on efficiency and the facilities a scalable system offers. In order to create a new computational system, research and possibilities for both hardware and software that offers scalability and its management have been carried out and explored. Given the extent of the development, as well as the complexity of the project that involves combining existing technology hardware with available software solutions, leaves further programming and research for future groups. In addition, this report includes a Business Model Canvas and Eco Design considerations regarding the future plans and environmental aspects for Teseus. The methodology used as a project management tool in order to organize the project and description of the Urban Node projects relation to Teseus, is also included.