1. Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribosomal DNA.
- Author
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Motoyama AB, Venancio EJ, Brandão GO, Petrofeza-Silva S, Pereira IS, Soares CM, and Felipe MS
- Subjects
- Base Sequence, DNA, Ribosomal analysis, Genes, rRNA, Humans, Molecular Sequence Data, RNA, Ribosomal, 28S genetics, RNA, Ribosomal, 5.8S genetics, DNA, Ribosomal genetics, Paracoccidioides classification, Paracoccidioides genetics, Paracoccidioidomycosis microbiology, Polymerase Chain Reaction methods
- Abstract
We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular epidemiological studies of P. brasiliensis in Latin America.
- Published
- 2000
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