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145 results on '"Petroutsos, Dimitris"'

1. Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging

Author

Ezzedine, Jade A., Uwizeye, Clarisse, Si Larbi, Grégory, Villain, Gaelle, Louwagie, Mathilde, Schilling, Marion, Hagenmuller, Pascal, Gallet, Benoît, Stewart, Adeline, Petroutsos, Dimitris, Devime, Fabienne, Salze, Pascal, Liger, Lucie, Jouhet, Juliette, Dumont, Marie, Ravanel, Stéphane, Amato, Alberto, Valay, Jean-Gabriel, Jouneau, Pierre-Henri, Falconet, Denis, and Maréchal, Eric

Published
2023
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3. Light-independent regulation of algal photoprotection by CO2 availability

Author

Ruiz-Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R., and Petroutsos, Dimitris

Published
2023
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4. Phototropin connects blue light perception to starch metabolism in green algae

Author

Yuan, Yizhong, primary, Iannetta, Anthony A, additional, Kim, Minjae, additional, Sadecki, Patric W., additional, Arend, Marius, additional, Tsichla, Angeliki, additional, Ruiz-Sola, M. Águila, additional, Kepesidis, Georgios, additional, Falconet, Denis, additional, Thevenon, Emmanuel, additional, Tardif, Marianne, additional, Brugière, Sabine, additional, Couté, Yohann, additional, Kleman, Jean Philippe, additional, Sizova, Irina, additional, Schilling, Marion, additional, Jouhet, Juliette, additional, Hegemann, Peter, additional, Li-Beisson, Yonghua, additional, Nikoloski, Zoran, additional, Bastien, Olivier, additional, Hicks, Leslie M., additional, and Petroutsos, Dimitris, additional

Published
2024
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6. Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Singh, Dipali, Dal Bo, Davide, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Le Monnier, Adeline, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Published
2017

7. Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging

Author

Marechal, Eric, primary, Ezzedine, Jade, additional, Uwizeye, Clarisse, additional, Larbi, Gregory Si, additional, Villain, Gaelle, additional, Louwagie, Mathilde, additional, Schilling, Marion, additional, Hagenmuller, Pascal, additional, Gallet, Benoit, additional, Stewart, Adeline, additional, Petroutsos, Dimitris, additional, Devime, Fabienne, additional, Salze, Pascal, additional, Liger, Lucie, additional, Jouhet, Juliette, additional, Dumont, Marie, additional, Ravanel, Stéphane, additional, Amato, Alberto, additional, Valay, Jean-Gabriel, additional, Jouneau, Pierre-Henri, additional, and Falconet, Denis, additional

Published
2023
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8. Light-independent regulation of algal photoprotection by CO2 availability

Author

Ruiz Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz Luque, Emanuel, Redekop, Petra, Petroutsos, Dimitris, Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Human Frontiers Science Program, French National Research Agency, Prestige Marie-Curie co-financing grant, European Union (UE). H2020, Carneige Institution for Science, Marie Curie Initial Training Network Accliphot, Japan Society for the Promotion of Science, and Deutsche Forschungsgemeinschaft / German Research Foundation (DFG)

Abstract

Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels. Human Frontiers Science Program RGP0046/2018 French National Research Agency ANR-18-CE20-0006, ANR-17-EURE-0003, ANR-15-IDEX-02 Prestige Marie-Curie co-financing grant PRESTIGE-2017-1-0028 European Union’s Horizon 2020 751039 Carnegie Institution for Science DE-SC0019417 Marie Curie Initial Training Network Accliphot FP7-PEPOPLE-2012-ITN, 316427 Japan Society for the Promotion of Science 21H04778, 21H05040 German Research Foundation HI 739/9.2

Published
2023

11. Photoprotection is regulated by light-independent CO2 availability

Author

Petroutsos, Dimitris, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience

Published
2022

12. Light-independent regulation of algal photoprotection by CO2 availability

Author

Ruiz-Sola, M. Águila, primary, Flori, Serena, additional, Yuan, Yizhong, additional, Villain, Gaelle, additional, Sanz-Luque, Emanuel, additional, Redekop, Petra, additional, Tokutsu, Ryutaro, additional, Kueken, Anika, additional, Tsichla, Angeliki, additional, Kepesidis, Georgios, additional, Allorent, Guillaume, additional, Arend, Marius, additional, Iacono, Fabrizio, additional, Finazzi, Giovanni, additional, Hippler, Michael, additional, Nikoloski, Zoran, additional, Minagawa, Jun, additional, Grossman, Arthur, additional, and Petroutsos, Dimitris, additional

Published
2022
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17. Light-independent regulation of algal photoprotection by CO2 availability.

Author

Águila Ruiz-Sola, M., Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Küken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur R., and Petroutsos, Dimitris

Subjects
CHLAMYDOMONAS reinhardtii, CHLAMYDOMONAS, ALGAE, PHOTOBIOLOGY
Abstract

Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. When light energy exceeds CO2 fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 and PSBS, and the CO2 Concentrating Mechanism (CCM). How light and CO2 signals converge to regulate these processes remains unclear. Here, we show that excess light activates photoprotection- and CCM-related genes by altering intracellular CO2 concentrations and that depletion of CO2 drives these responses, even in total darkness. High CO2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3/CCM genes while stabilizing the LHCSR1 protein. Finally, we show that the CCM regulator CIA5 also regulates photoprotection, controlling LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 protein accumulation. This work has allowed us to dissect the effect of CO2 and light on CCM and photoprotection, demonstrating that light often indirectly affects these processes by impacting intracellular CO2 levels. Photosynthetic algae have evolved to survive in suboptimal light and CO2 conditions. Here, the authors show that depletion of CO2 can drive photoprotection and carbon acquisition even in the absence of light, that was previously believed to be indispensable for the activation of these processes. [ABSTRACT FROM AUTHOR]

Published
2023
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21. A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii

Author

Allorent, Guillaume, Tokutsu, Ryutaro, Roach, Thomas, Peers, Graham, Cardol, Pierre, Girard-Bascou, Jacqueline, Seigneurin-Berny, Daphné, Petroutsos, Dimitris, Kuntz, Marcel, Breyton, Cécile, Franck, Fabrice, Wollman, Francis-André, Niyogi, Krishna K., Krieger-Liszkay, Anja, Minagawa, Jun, and Finazzi, Giovanni

Published
2013

22. Phototropin Links Blue-Light Perception with Starch Accumulation in Chlamydomonas

Author

Petroutsos, Dimitris, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience

Published
2022

24. Energetic coupling between plastids and mitochondria drives C[O.sub.2] assimilation in diatoms

Author

Bailleul, Benjamin, Berne, Nicolas, Murik, Omer, Petroutsos, Dimitris, Prihoda, Judit, Tanaka, Atsuko, Villanova, Valeria, Bligny, Richard, Flori, Serena, Falconet, Denis, Krieger-Liszkay, Anja, Santabarbara, Stefano, Rappaport, Fabrice, Joliot, Pierre, Tirichine, Leila, Falkowski, Paul G., Cardol, Pierre, Bowler, Chris, and Finazzi, Giovanni

Subjects
Diatoms -- Research, Mitochondria -- Physiological aspects, Photosynthesis -- Research, Phytochemistry -- Research, Botanical research, Plastids -- Physiological aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Diatoms are one of the most ecologically successful classes of photosynthetic marine eukaryotes in the contemporary oceans. Over the past 30 million years, they have helped to moderate Earth's climate [...]

Published
2015

28. Photoprotection is regulated by light-independent CO 2 availability

Author

Petroutsos, Dimitris, Ruiz-Sola, M. Águila, Flori, Serena, Yuan, Yizhong, Villain, Gaelle, Sanz-Luque, Emanuel, Redekop, Petra, Tokutsu, Ryutaro, Kueken, Anika, Tsichla, Angeliki, Kepesidis, Georgios, Allorent, Guillaume, Arend, Marius, Iacono, Fabrizio, Finazzi, Giovanni, Hippler, Michael, Nikoloski, Zoran, Minagawa, Jun, Grossman, Arthur, Physiologie cellulaire et végétale (LPCV), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Abstract

Photosynthetic algae cope with suboptimal levels of light and CO 2 . In low CO 2 and excess light, the green alga Chlamydomonas reinhardtii activates a CO 2 Concentrating Mechanism (CCM) and photoprotection; the latter is mediated by LHCSR1/3 and PSBS. How light and CO 2 signals converge to regulate photoprotective responses remains unclear. Here we show that excess light activates expression of photoprotection- and CCM-related genes and that depletion of CO 2 drives these responses, even in total darkness. High CO 2 levels, derived from respiration or impaired photosynthetic fixation, repress LHCSR3 and CCM genes while stabilizing the LHCSR1 protein. We also show that CIA5, which controls CCM genes, is a major regulator of photoprotection, elevating LHCSR3 and PSBS transcript accumulation while inhibiting LHCSR1 accumulation. Our work emphasizes the importance of CO 2 in regulating photoprotection and the CCM, demonstrating that the impact of light on photoprotection is often indirect and reflects intracellular CO 2 levels.

Published
2021

30. Light-independent regulation of algal photoprotection by CO2availability

Author

Ruiz-Sola, M. Águila, primary, Flori, Serena, additional, Yuan, Yizhong, additional, Villain, Gaelle, additional, Sanz-Luque, Emanuel, additional, Redekop, Petra, additional, Tokutsu, Ryutaro, additional, Kueken, Anika, additional, Tsichla, Angeliki, additional, Kepesidis, Georgios, additional, Allorent, Guillaume, additional, Arend, Marius, additional, Iacono, Fabrizio, additional, Finazzi, Giovanni, additional, Hippler, Michael, additional, Nikoloski, Zoran, additional, Minagawa, Jun, additional, Grossman, Arthur R., additional, and Petroutsos, Dimitris, additional

Published
2021
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31. Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches

Author

Bo, Davide Dal, Magneschi, Leonardo, Bedhomme, Mariette, Billey, Elodie, Deragon, Etienne, Storti, Mattia, Menneteau, Mathilde, Richard, Christelle, Rak, Camille, Lapeyre, Morgane, Lembrouk, Mehdi, Conte, Melissa, Gros, Valérie, Tourcier, Guillaume, Giustini, Cécile, Falconet, Denis, Curien, Gilles, Allorent, Guillaume, Petroutsos, Dimitris, Laeuffer, Frédéric, Fourage, Laurent, Jouhet, Juliette, Maréchal, Eric, Finazzi, Giovanni, Collin, Séverine, Light Photosynthesis & Metabolism (Photosynthesis), Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), LIPID, Total Refining Chemicals, Détection et signalisation dans les microalgues (Signal), Federation de Recherche GO-SEE, CEA-Total partnership, ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), and European Project: 833184, ChloroMito

Subjects
photosynthesis, mixotrophy, mitochondrial alternative oxidase, lipid metabolism, Microchloropsis gaditana, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, TALE nuclease
Abstract

International audience; Algae belonging to the Microchloropsis genus are promising organisms for biotech purposes, being able to accumulate large amounts of lipid reserves. These organisms adapt to different trophic conditions, thriving in strict photoautotrophic conditions, as well as in the concomitant presence of light plus reduced external carbon as energy sources (mixotrophy). In this work, we investigated the mixotrophic responses of Microchloropsis gaditana (formerly Nannochloropsis gaditana ). Using the Biolog growth test, in which cells are loaded into multiwell plates coated with different organic compounds, we could not find a suitable substrate for Microchloropsis mixotrophy. By contrast, addition of the Lysogeny broth (LB) to the inorganic growth medium had a benefit on growth, enhancing respiratory activity at the expense of photosynthetic performances. To further dissect the role of respiration in Microchloropsis mixotrophy, we focused on the mitochondrial alternative oxidase (AOX), a protein involved in energy management in other algae prospering in mixotrophy. Knocking-out the AOX1 gene by transcription activator-like effector nuclease (TALE-N) led to the loss of capacity to implement growth upon addition of LB supporting the hypothesis that the effect of this medium was related to a provision of reduced carbon. We conclude that mixotrophic growth in Microchloropsis is dominated by respiratory rather than by photosynthetic energetic metabolism and discuss the possible reasons for this behavior in relationship with fatty acid breakdown via β-oxidation in this oleaginous alga.

Published
2021

35. Supplementary Fig. 1 Quantitative analysis of P. tricornutum glycerolipids from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Dipali Singh, Bo, Davide Dal, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Monnier, Adeline Le, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Abstract

TAG profile in a total lipid extract from cells grown in replete conditions (A) and deplete conditions (B) in both mixotrophic and phototrophic mode. Glycerolipids are expressed in nmol / mg of dry cells. Each result is the average of two biological replicates ± SD. PHOT: light in N-replete condition; PHOTO-N: light in N-deplete condi-tion; MIX: light+glycerol in N-replete condition; MIX-N: light+glycerol in N-deplete condition.

Published
2017
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36. Supplementary Fig. 3 Quantification of intracellular pyruvate by a fluorescence-based method from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Dipali Singh, Bo, Davide Dal, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Monnier, Adeline Le, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Abstract

A. Pyruvate standard curve. B. Quantification of intracellular pyruvate in cells grown in phototrophy (PHOT) and mixotrophy (MIX).

Published
2017
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37. Supplementary Fig. 2 Membrane lipid composition in P. tricornutum from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Dipali Singh, Bo, Davide Dal, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Monnier, Adeline Le, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Abstract

Lipid analysis of cells grow in N-replete conditions and N-deplete conditions in both mixotrophic and phototrophic mode. Each result is the average of two biological replicates ± SD. SQDG, sulfoquinovosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; MGDG, monogalactosyldiacylglycerol; PC, phosphatidylcholine; PHOT: light in N-replete condition; PHOTO-N: light in N-deplete condition; MIX: light+glycerol in N-replete condition; MIX-N: light+glycerol in N-deplete condition.

Published
2017
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38. Supplementary Fig. 4 A Respiration and photosynthesis in P. tricornutum cells from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Dipali Singh, Bo, Davide Dal, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Monnier, Adeline Le, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Abstract

Direct assessment of oxygen consumption by a polarographic approach in both phototrophy (black bar) and mix-otrophy (red bar). B. Fluorescent based-assay to monitoring the changes in respiration using the Redox Dye A in presence of the selected compounds (see methods).

Published
2017
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39. Supplementary Fig. 5 Screening of mixotrophic efficiency by biolog and redox dye assay in P. tricornutum from Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Author

Villanova, Valeria, Fortunato, Antonio Emidio, Dipali Singh, Bo, Davide Dal, Conte, Melissa, Obata, Toshihiro, Jouhet, Juliette, Fernie, Alisdair R., Marechal, Eric, Falciatore, Angela, Pagliardini, Julien, Monnier, Adeline Le, Poolman, Mark, Curien, Gilles, Petroutsos, Dimitris, and Finazzi, Giovanni

Abstract

A. OD750 nm changes (relative to phototrophic growth) of P. tricornutum cells grown for 6 days in BiologTM plates P1 and PM2A that contains 190 carbon compounds (see methods). Each data point represents a different com-pound. B. Growth profile of P. tricornutum on few selected compounds (at 20 mM) and a phototrophic control in 100 mL flasks. C. Areas under the growth curves of Supplementary Fig. 5B normalized to the area of the curve of phototrophic growth.

Published
2017
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40. Plastid thylakoid architecture optimizes photosynthesis in diatoms

Author

Flori, Serena, Jouneau, Pierre-Henri, Bailleul, Benjamin, Gallet, Benoit, Estrozi, Leandro F, Moriscot, Christine, Bastien, Olivier, Eicke, Simona, Schober, Alexander, Bártulos, Carolina Río, Maréchal, Eric, Kroth, Peter G, Petroutsos, Dimitris, Zeeman, Samuel, Breyton, Cécile, Schoehn, Guy, Falconet, Denis, Finazzi, Giovanni, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'Etude des Matériaux par Microscopie Avancée (LEMMA), Modélisation et Exploration des Matériaux (MEM), Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Physiologie membranaire et moléculaire du chloroplaste (PMMC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de biologie structurale (IBS - UMR 5075), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS), Plant Biochemistry, Department of Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich] (ETH Zürich), Department of Biology, University of Konstanz, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Laboratoire d'Etude des Matériaux par Microscopie Avancée (LEMMA ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Graduate School of Chemical Biology (KoRS-CB), FRISBI within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-INSB-05-02], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Diatoms, Chloroplasts, Photosystem I Protein Complex, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Science, Photosystem II Protein Complex, Thylakoids, Article, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], ddc:570, Plastids, Photosynthesis, Electron microscopy
Abstract

Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in plants, we are still unlocking the mechanisms evolved by phytoplankton to achieve extremely efficient photosynthesis. Here, we combine biochemical, structural and in vivo physiological studies to unravel the structure of the plastid in diatoms, prominent marine eukaryotes. Biochemical and immunolocalization analyses reveal segregation of photosynthetic complexes in the loosely stacked thylakoid membranes typical of diatoms. Separation of photosystems within subdomains minimizes their physical contacts, as required for improved light utilization. Chloroplast 3D reconstruction and in vivo spectroscopy show that these subdomains are interconnected, ensuring fast equilibration of electron carriers for efficient optimum photosynthesis. Thus, diatoms and plants have converged towards a similar functional distribution of the photosystems although via different thylakoid architectures, which likely evolved independently in the land and the ocean., Phytoplankton and plant plastids have distinct evolutionary origins and membrane organization. Here Flori et al. show that diatom photosynthetic complexes spatially segregate into interconnected subdomains within loose thylakoid stacks enabling fast diffusion of electron carriers and efficient photosynthesis

Published
2017

42. Use of fib‐sem to address the ultrastructure of the periplastidial and internal chloroplast compartments of the diatom Phaeodactylum tricornutum

Author

Flori, Serena, primary, Jouneau, Pierre‐Henri, additional, Gallet, Benoit, additional, Moriscot, Christine, additional, Estrozi, Leandro, additional, Petroutsos, Dimitris, additional, Breyton, Cécile, additional, Schoehn, Guy, additional, Maréchal, Eric, additional, Finazzi, Giovanni, additional, and Falconet, Denis, additional

Published
2016
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44. Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast

Author

Hochmal, Ana Karina, primary, Zinzius, Karen, additional, Charoenwattanasatien, Ratana, additional, Gäbelein, Philipp, additional, Mutoh, Risa, additional, Tanaka, Hideaki, additional, Schulze, Stefan, additional, Liu, Gai, additional, Scholz, Martin, additional, Nordhues, André, additional, Offenborn, Jan Niklas, additional, Petroutsos, Dimitris, additional, Finazzi, Giovanni, additional, Fufezan, Christian, additional, Huang, Kaiyao, additional, Kurisu, Genji, additional, and Hippler, Michael, additional

Published
2016
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45. The Water to Water Cycles in Microalgae

Author

Curien, Gilles, primary, Flori, Serena, additional, Villanova, Valeria, additional, Magneschi, Leonardo, additional, Giustini, Cécile, additional, Forti, Giorgio, additional, Matringe, Michel, additional, Petroutsos, Dimitris, additional, Kuntz, Marcel, additional, and Finazzi, Giovanni, additional

Published
2016
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46. Ions channels/transporters and chloroplast regulation

Author

Finazzi, Giovanni, primary, Petroutsos, Dimitris, additional, Tomizioli, Martino, additional, Flori, Serena, additional, Sautron, Emeline, additional, Villanova, Valeria, additional, Rolland, Norbert, additional, and Seigneurin-Berny, Daphné, additional

Published
2015
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47. Membrane Glycerolipid Remodeling Triggered by Nitrogen and Phosphorus Starvation inPhaeodactylum tricornutum

Author

Abida, Heni, primary, Dolch, Lina-Juana, additional, Meï, Coline, additional, Villanova, Valeria, additional, Conte, Melissa, additional, Block, Maryse A., additional, Finazzi, Giovanni, additional, Bastien, Olivier, additional, Tirichine, Leïla, additional, Bowler, Chris, additional, Rébeillé, Fabrice, additional, Petroutsos, Dimitris, additional, Jouhet, Juliette, additional, and Maréchal, Eric, additional

Published
2014
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48. Evolution of galactoglycerolipid biosynthetic pathways – From cyanobacteria to primary plastids and from primary to secondary plastids

Author

Petroutsos, Dimitris, primary, Amiar, Souad, additional, Abida, Heni, additional, Dolch, Lina-Juana, additional, Bastien, Olivier, additional, Rébeillé, Fabrice, additional, Jouhet, Juliette, additional, Falconet, Denis, additional, Block, Maryse A., additional, McFadden, Geoffrey I., additional, Bowler, Chris, additional, Botté, Cyrille, additional, and Maréchal, Eric, additional

Published
2014
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49. Glycerolipids in photosynthesis: Composition, synthesis and trafficking

Author

Boudière, Laurence, primary, Michaud, Morgane, additional, Petroutsos, Dimitris, additional, Rébeillé, Fabrice, additional, Falconet, Denis, additional, Bastien, Olivier, additional, Roy, Sylvaine, additional, Finazzi, Giovanni, additional, Rolland, Norbert, additional, Jouhet, Juliette, additional, Block, Maryse A., additional, and Maréchal, Eric, additional

Published
2014
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50. Proton Gradient Regulation 5-Mediated Cyclic Electron Flow under ATP- or Redox-Limited Conditions: A Study of ƊATPase pgr5 and ƊrbcL pgr5 Mutants in the Green Alga Chlamydomonas reinhardtii

Author

Johnson, Xenie, primary, Steinbeck, Janina, additional, Dent, Rachel M., additional, Takahashi, Hiroko, additional, Richaud, Pierre, additional, Ozawa, Shin-Ichiro, additional, Houille-Vernes, Laura, additional, Petroutsos, Dimitris, additional, Rappaport, Fabrice, additional, Grossman, Arthur R., additional, Niyogi, Krishna K., additional, Hippler, Michael, additional, and Alric, Jean, additional

Published
2014
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