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2. Transmission of yellow fever vaccine virus through blood transfusion and organ transplantation in the USA in 2021: report of an investigation

Author

Ajaimy, Maria, Al Azzi, Yorg, Annambhotla, Pallavi, Backenson, Bryon, Belden, Katherine, Colovai, Adriana, Coppock, Dagan, Du, Lydia, Estetter, Lindsey, Le, Marie, Martines, Roosecelis B., Pettengill, Matthew, Szymczak, Wendy, Vareechon, Chairut, Gould, Carolyn V, Free, Rebecca J, Bhatnagar, Julu, Soto, Raymond A, Royer, Tricia L, Maley, Warren R, Moss, Sean, Berk, Matthew A, Craig-Shapiro, Rebecca, Kodiyanplakkal, Rosy Priya L, Westblade, Lars F, Muthukumar, Thangamani, Puius, Yoram A, Raina, Amresh, Hadi, Azam, Gyure, Kymberly A, Trief, Danielle, Pereira, Marcus, Kuehnert, Matthew J, Ballen, Vennus, Kessler, Debra A, Dailey, Kimberly, Omura, Charles, Doan, Thuy, Miller, Steve, Wilson, Michael R, Lehman, Jennifer A, Ritter, Jana M, Lee, Elizabeth, Silva-Flannery, Luciana, Reagan-Steiner, Sarah, Velez, Jason O, Laven, Janeen J, Fitzpatrick, Kelly A, Panella, Amanda, Davis, Emily H, Hughes, Holly R, Brault, Aaron C, St George, Kirsten, Dean, Amy B, Ackelsberg, Joel, Basavaraju, Sridhar V, Chiu, Charles Y, and Staples, J Erin

Published
2023
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4. Danger signals, inflammasomes, and the intricate intracellular lives of chlamydiae.

Author

Pettengill, Matthew A, Abdul-Sater, Ali, Coutinho-Silva, Robson, and Ojcius, David M

Subjects
Humans, Chlamydiaceae Infections, Signal Transduction, Inflammasomes, Chlamydia, Immunology, Infection, Inflammation, Innate immunity
Abstract

Chlamydiae are obligate intracellular bacterial pathogens, and as such are sensitive to alterations in the cellular physiology of their hosts. Chlamydial infections often cause pathologic consequences due to prolonged localized inflammation. Considerable advances have been made in the last few years regarding our understanding of how two key inflammation-associated signaling pathways influence the biology of Chlamydia infections: inflammation regulating purinergic signaling pathways significantly impact intracellular chlamydial development, and inflammasome activation modulates both chlamydial growth and infection mediated pro-inflammatory cytokine production. We review here elements of both pathways, presenting the latest developments contributing to our understanding of how chlamydial infections are influenced by inflammasomes and purinergic signaling.

Published
2016

6. Commercial Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Molecular Assays: Superior Analytical Sensitivity of cobas SARS-CoV-2 Relative to NxTAG CoV Extended Panel and ID NOW COVID-19 Test

Author

Jin, Run, Pettengill, Matthew A., Hartnett, Nicole L., Auerbach, Herbert E., Peiper, Stephen C., and Wang, Zixuan

Subjects
Molecular diagnostic techniques -- Comparative analysis, Biochemical assays -- Comparative analysis, COVID-19 -- Diagnosis, Health
Abstract

* Context.--We implemented multiple nucleic acid amplification test platforms because of the limited availability of test kits for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the early stages of the pandemic. Interpretation of results generated by different platforms and prioritization for testing algorithms required cross-comparison. Objective.--To compare the analytical sensitivity of 3 commercial SARS-CoV-2 molecular assays, selected samples were studied in parallel with Cobas SARS-CoV-2 test, NxTAG CoV Extended Panel, and ID NOW COVID-19 assays. Design.--A total of 8043 SARS-CoV-2 tests performed from March 22 to April 19, 2020, were included in this study. For all 1794 positive specimens detected by the cobas SARS-CoV-2 assay, the cycle threshold ([C.sub.t]) values were manually tracked and plotted to demonstrate the distribution of sample viral levels. Additionally, 50 and 63 low-positive specimens ([C.sub.t] values >32) as well as 50 and 61 consecutive positive specimens by the cobas assay were tested with NxTAG and ID NOW, respectively, to estimate their relative sensitivities. Results.--The [C.sub.t] values of cobas SARS-CoV-2-positive samples were evenly distributed throughout ranges of 13.32 to 39.50 (mean, 25.06) and 13.60 to 42.49 (mean, 26.45) for ORF1 and E gene targets, respectively. NxTAG reliably detected only specimens with E gene [C.sub.t] values lower than 33, and is estimated to detect 89.4% of positive specimens detected by cobas assay. ID NOW had performance variation independent of [C.sub.t] value and is estimated to detect 83.5% of cobas positives. Conclusions.--Clinical specimens exhibit a wide range of viral burden, with a significant portion at low levels. Analytical sensitivity of testing platforms is critical for reliable detection of SARS-CoV-2 and uniform care to patients. doi: 10.5858/arpa.2020-0283-SA, Severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) is an enveloped RNA virus that causes a spectrum of health outcomes ranging from asymptomatic infection to severe respiratory illness that may lead [...]

Published
2020
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10. Transmission of yellow fever vaccine virus through blood transfusion and organ transplantation in the USA in 2021: report of an investigation

Author

Gould, Carolyn V, primary, Free, Rebecca J, additional, Bhatnagar, Julu, additional, Soto, Raymond A, additional, Royer, Tricia L, additional, Maley, Warren R, additional, Moss, Sean, additional, Berk, Matthew A, additional, Craig-Schapiro, Rebecca, additional, Kodiyanplakkal, Rosy Priya L, additional, Westblade, Lars F, additional, Muthukumar, Thangamani, additional, Puius, Yoram A, additional, Raina, Amresh, additional, Hadi, Azam, additional, Gyure, Kymberly A, additional, Trief, Danielle, additional, Pereira, Marcus, additional, Kuehnert, Matthew J, additional, Ballen, Vennus, additional, Kessler, Debra A, additional, Dailey, Kimberly, additional, Omura, Charles, additional, Doan, Thuy, additional, Miller, Steve, additional, Wilson, Michael R, additional, Lehman, Jennifer A, additional, Ritter, Jana M, additional, Lee, Elizabeth, additional, Silva-Flannery, Luciana, additional, Reagan-Steiner, Sarah, additional, Velez, Jason O, additional, Laven, Janeen J, additional, Fitzpatrick, Kelly A, additional, Panella, Amanda, additional, Davis, Emily H, additional, Hughes, Holly R, additional, Brault, Aaron C, additional, St George, Kirsten, additional, Dean, Amy B, additional, Ackelsberg, Joel, additional, Basavaraju, Sridhar V, additional, Chiu, Charles Y, additional, Staples, J Erin, additional, Ajaimy, Maria, additional, Al Azzi, Yorg, additional, Annambhotla, Pallavi, additional, Backenson, Bryon, additional, Belden, Katherine, additional, Colovai, Adriana, additional, Coppock, Dagan, additional, Du, Lydia, additional, Estetter, Lindsey, additional, Le, Marie, additional, Martines, Roosecelis B., additional, Pettengill, Matthew, additional, Szymczak, Wendy, additional, and Vareechon, Chairut, additional

Published
2023
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12. Outbreak of Burkholderia stabilis Infections Associated with Contaminated Nonsterile, Multiuse Ultrasound Gel-10 States, May-September 2021

Author

Hudson, Matthew J., Park, Stacy C., Mathers, Amy, Parikh, Hardik, Glowicz, Janet, Dar, David, Nabili, Marjan, LiPuma, John J., Bumford, Amy, Pettengill, Matthew A., Sterner, Mark R., Jr., Paoline, Julie, Tressler, Stacy, Peritz, Tiina, Gould, Jane, Hutter, Stuart R., Moulton-Meissner, Heather, and Perkins, Kiran M.

Subjects
United States. Food and Drug Administration, Genomics -- Health aspects, Infection -- Health aspects, Medical colleges -- Health aspects, Hospital patients -- Health aspects, Health
Abstract

In July 2021, the Virginia Department of Health notified CDC of a cluster of eight invasive infections with Burkholderia stabilis, a bacterium in the Burkholderia cepacia complex (BCC), among hospitalized [...]

Published
2022

17. Dynamic molecular changes during the first week of human life follow a robust developmental trajectory

Author

Lee, Amy H., Shannon, Casey P., Amenyogbe, Nelly, Bennike, Tue B., Diray-Arce, Joann, Idoko, Olubukola T., Gill, Erin E., Ben-Othman, Rym, Pomat, William S., van Haren, Simon D., Cao, Kim-Anh Lê, Cox, Momoudou, Darboe, Alansana, Falsafi, Reza, Ferrari, Davide, Harbeson, Daniel J., He, Daniel, Bing, Cai, Hinshaw, Samuel J., Ndure, Jorjoh, Njie-Jobe, Jainaba, Pettengill, Matthew A., Richmond, Peter C., Ford, Rebecca, Saleu, Gerard, Masiria, Geraldine, Matlam, John Paul, Kirarock, Wendy, Roberts, Elishia, Malek, Mehrnoush, Sanchez-Schmitz, Guzmán, Singh, Amrit, Angelidou, Asimenia, Smolen, Kinga K., The EPIC Consortium, Brinkman, Ryan R., Ozonoff, Al, Hancock, Robert E. W., van den Biggelaar, Anita H. J., Steen, Hanno, Tebbutt, Scott J., Kampmann, Beate, Levy, Ofer, and Kollmann, Tobias R.

Published
2019
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18. Bacille Calmette-Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro

Author

Diray-Arce, Joann, Angelidou, Asimenia, Jensen, Kristoffer Jarlov, Conti, Maria Giulia, Kelly, Rachel S, Pettengill, Matthew A, Liu, Mark, van Haren, Simon D, McCulloch, Scott D, Michelloti, Greg, Idoko, Olubukola, EPIC Consortium, Kollmann, Tobias R, Kampmann, Beate, Steen, Hanno, Ozonoff, Al, Lasky-Su, Jessica, Benn, Christine S, and Levy, Ofer

Abstract

Vaccines have generally been developed with limited insight into their molecular impact. While systems vaccinology enables characterization of mechanisms of action, these tools have yet to be applied to infants, who are at high risk of infection and receive the most vaccines. Bacille Calmette-Guérin (BCG) protects infants against disseminated tuberculosis (TB) and TB-unrelated infections via incompletely understood mechanisms. We employ mass-spectrometry-based metabolomics of blood plasma to profile BCG-induced infant responses in Guinea-Bissau in vivo and the US in vitro. BCG-induced lysophosphatidylcholines (LPCs) correlate with both TLR-agonist- and purified protein derivative (PPD, mycobacterial antigen)-induced blood cytokine production in vitro, raising the possibility that LPCs contribute to BCG immunogenicity. Analysis of an independent newborn cohort from The Gambia demonstrates shared vaccine-induced metabolites, such as phospholipids and sphingolipids. BCG-induced changes to the plasma lipidome and LPCs may contribute to its immunogenicity and inform the development of early life vaccines.

Published
2022

27. 3D Printed Alternative to the Standard Synthetic Flocked Nasopharyngeal Swabs Used for COVID-19 testing

Author

Decker, Summer J, Goldstein, Todd A, Ford, Jonathan M, Teng, Michael N, Pugliese, Robert S, Berry, Gregory J, Pettengill, Matthew, Silbert, Suzane, Hazelton, Todd R, Wilson, Jason W, Shine, Kristy, Wang, Zi-Xuan, Hutchinson, Morgan, Castagnaro, Joseph, Bloom, Ona E, Breining, Dwayne A, Goldsmith, Barbara M, Sinnott, John T, O'Donnell, Donna Gentile, Crawford, James M, Lockwood, Charles J, and Kim, Kami

Subjects
molecular diagnostics, AcademicSubjects/MED00290, COVID-19 Testing, SARS-CoV-2, 3D-printed swabs, nasopharyngeal, Nasopharynx, Printing, Three-Dimensional, Major Article, COVID-19, Humans
Abstract

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, can be detected in respiratory samples by Real-time Reverse Transcriptase (RT)-PCR or other molecular methods. Accessibility of diagnostic testing for COVID-19 has been limited by intermittent shortages of supplies required for testing, including flocked nasopharyngeal (FLNP) swabs. Methods We developed a 3D-printed nasopharyngeal (3DP) swab as a replacement of the FLNP swab. The performance of 3DP and FLNP swabs were compared in a clinical trial of symptomatic patients at three clinical sites (n=291) using three SARS-CoV-2 EUA tests: a modified version of the CDC Real-time Reverse Transcriptase (RT)-PCR Diagnostic Panel and two commercial automated formats, Roche Cobas and NeuMoDx. Results The cycle threshold (C(t)) values from the gene targets and the RNase P gene control in the CDC assay showed no significant differences between swabs for both gene targets (p=0.152 and p=0.092), with the RNase P target performing significantly better in the 3DP swabs (p & 0.001). The C(t) values showed no significant differences between swabs for both viral gene targets in the Roche cobas assay (p=0.05 and p=0.05) as well as the NeuMoDx assay (p=0.401 and p=0.484). The overall clinical correlation of COVID-19 diagnosis between all methods was 95.88% (Kappa 0.901). Conclusions 3DP swabs were equivalent to standard FLNP in three testing platforms for SARS-CoV-2. Given the need for widespread testing, 3DP swabs printed on-site are an alternate to FLNP that can rapidly scale in response to acute needs when supply chain disruptions affect availability of collection kits.

Published
2020

33. Danger signals and the regulation of Chlamydia infection in epithelial cells

Author

Pettengill, Matthew Aaron

Subjects
Biology., Chlamydia., Epithelial cells.
Abstract

Bacterial infections of the mucosal epithelium initiate inflammation of the local tissues, which leads tochanges in the molecular profile of the extracellular milieu, including increases in extracellular ATP, andits ecto-enzymatic and hydrolytic degradation products ADP and adenosine. Extracellular ATP andadenosine function as signaling molecules at purinergic receptors which have been shown to regulateseveral key aspects of immune cell function. Information has been quite limited, however, on how thismay alter bacterial growth in the inflamed tissue. Here we demonstrate that stimulation of Chlamydiatrachomatis infected epithelial cells with micromolar extracellular ATP, ADP and adenosine significantlyimpairs growth of the bacteria. The response to adenosine is mediated by the A2b receptor, while theeffects of ATP and ADP suggest involvement of P2X4 receptors. Chlamydial growth is reversiblyinhibited, and the bacteria return to normal growth kinetics following removal of the stimulus. Thissuggests that extracellular adenosine and adenine nucleotides are important for local control of C.trachomatis at infected mucosal epithelium, and that further characterization of the intracellularmechanisms employed by the host could lead to improved methods of treating this important pathogen.

Published
2010

34. 3-Dimensional Printed Alternative to the Standard Synthetic Flocked Nasopharyngeal Swabs Used for Coronavirus Disease 2019 Testing

Author

Decker, Summer J, primary, Goldstein, Todd A, additional, Ford, Jonathan M, additional, Teng, Michael N, additional, Pugliese, Robert S, additional, Berry, Gregory J, additional, Pettengill, Matthew, additional, Silbert, Suzane, additional, Hazelton, Todd R, additional, Wilson, Jason W, additional, Shine, Kristy, additional, Wang, Zi-Xuan, additional, Hutchinson, Morgan, additional, Castagnaro, Joseph, additional, Bloom, Ona E, additional, Breining, Dwayne A, additional, Goldsmith, Barbara M, additional, Sinnott, John T, additional, O’Donnell, Donna Gentile, additional, Crawford, James M, additional, Lockwood, Charles J, additional, and Kim, Kami, additional

Published
2020
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35. Precision immunology for discovery and development of the Precision Vaccines Program (PVP)-037 small molecule series: imidazopyrimidine adjuvants identified via age-specific human in vitro modeling

Author

Soni, Dheeraj, primary, Borriello, Francesco, additional, Scott, David A, additional, Ozonoff, Al, additional, Brightman, Spencer, additional, Smith, Jennifer, additional, Shamu, Caroline, additional, Ramirez, Juan C, additional, Baden, Lindsey Robert, additional, Cheng, Wing Ki, additional, van Haren, Simon Daniel, additional, Pettengill, Matthew, additional, Dowling, David J, additional, and Levy, Ofer, additional

Published
2020
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37. Bacillus Calmette-Guérin (BCG) vaccine reprograms human neonatal metabolism in vivo and in vitro

Author

Arce, Joann Diray, primary, Jensen, Kristoffer Jarlov, additional, Angelidou, Asimenia, additional, Conti, Maria Giulia, additional, Kelly, Rachel S., additional, Pettengill, Matthew, additional, Liu, Mark, additional, van Haren, Simon D., additional, Benn, Christine Stabell, additional, Ozonoff, Al, additional, Steen, Hanno, additional, Lasky-Su, Jessica, additional, and Levy, Ofer, additional

Published
2020
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41. 3-Dimensional Printed Alternative to the Standard Synthetic Flocked Nasopharyngeal Swabs Used for Coronavirus Disease 2019 Testing.

Author

Decker, Summer J, Goldstein, Todd A, Ford, Jonathan M, Teng, Michael N, Pugliese, Robert S, Berry, Gregory J, Pettengill, Matthew, Silbert, Suzane, Hazelton, Todd R, Wilson, Jason W, Shine, Kristy, Wang, Zi-Xuan, Hutchinson, Morgan, Castagnaro, Joseph, Bloom, Ona E, Breining, Dwayne A, Goldsmith, Barbara M, Sinnott, John T, O'Donnell, Donna Gentile, and Crawford, James M

Subjects
PREDICTIVE tests, CLINICAL trials, NASOPHARYNX, COMPARATIVE studies, GENES, DESCRIPTIVE statistics, COVID-19 testing, THREE-dimensional printing, POLYMERASE chain reaction
Abstract

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019 (COVID-19), can be detected in respiratory samples by real-time reverse transcriptase polymerase chain reaction (RT-PCR) or other molecular methods. Accessibility of diagnostic testing for COVID-19 has been limited by intermittent shortages of supplies required for testing, including flocked nasopharyngeal (FLNP) swabs. Methods We developed a 3-dimensional printed nasopharyngeal (3DP) swab as a replacement of the FLNP swab. The performance of 3DP and FLNP swabs were compared in a clinical trial of symptomatic patients at 3 clinical sites (n = 291) using 3 SARS-CoV-2 emergency use authorization tests: a modified version of the Centers for Disease Control and Prevention (CDC) RT-PCR Diagnostic Panel and 2 commercial automated formats, Roche Cobas and NeuMoDx. Results The cycle threshold—C(t)—values from the gene targets and the RNase P gene control in the CDC assay showed no significant differences between swabs for both gene targets (P  = .152 and P  = .092), with the RNase P target performing significantly better in the 3DP swabs (P  < .001). The C(t) values showed no significant differences between swabs for both viral gene targets in the Roche cobas assay (P  = .05 and P  = .05) as well as the NeuMoDx assay (P  = .401 and P  = .484). The overall clinical correlation of COVID-19 diagnosis between all methods was 95.88% (Kappa 0.901). Conclusions The 3DP swabs were equivalent to standard FLNP in 3 testing platforms for SARS-CoV-2. Given the need for widespread testing, 3DP swabs printed onsite are an alternate to FLNP that can rapidly scale in response to acute needs when supply chain disruptions affect availability of collection kits. [ABSTRACT FROM AUTHOR]

Published
2021
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42. Supplemental material for Distinct TLR-mediated cytokine production and immunoglobulin secretion in human newborn naïve B cells

Author

Pettengill, Matthew A, Haren, Simon D Van, Li, Ning, Dowling, David J, Bergelson, Ilana, Jans, Jop, Ferwerda, Gerben, and Levy, Ofer

Subjects
FOS: Clinical medicine, 110306 Endocrinology
Abstract

Supplemental Material for Distinct TLR-mediated cytokine production and immunoglobulin secretion in human newborn naïve B cells by Matthew A Pettengill, Simon D van Haren, Ning Li, David J Dowling, Ilana Bergelson, Jop Jans, Gerben Ferwerda and Ofer Levy in Innate Immunity

Published
2019
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49. Correction: Human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis

Author

Pettengill, Matthew, primary, Matute, Juan D., additional, Tresenriter, Megan, additional, Hibbert, Julie, additional, Burgner, David, additional, Richmond, Peter, additional, Millán, José Luis, additional, Ozonoff, Al, additional, Strunk, Tobias, additional, Currie, Andrew, additional, and Levy, Ofer, additional

Published
2018
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50. Adjuvant Effect of Bacille Calmette–Guérin on Hepatitis B Vaccine Immunogenicity in the Preterm and Term Newborn

Author

Scheid, Annette, primary, Borriello, Francesco, additional, Pietrasanta, Carlo, additional, Christou, Helen, additional, Diray-Arce, Joann, additional, Pettengill, Matthew A., additional, Joshi, Sweta, additional, Li, Ning, additional, Bergelson, Ilana, additional, Kollmann, Tobias, additional, Dowling, David J., additional, and Levy, Ofer, additional

Published
2018
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