4,685 results on '"Phagosome"'
Search Results
2. Humoral pathways of innate immune regulation in granuloma formation.
- Author
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Santos-Ribeiro, Diana, Cunha, Cristina, and Carvalho, Agostinho
- Subjects
- *
GRANULOMA , *THERAPEUTICS , *NATURAL immunity , *COMPLEMENT inhibition , *TISSUE remodeling - Abstract
Humoral innate immunity can regulate molecular and cellular events that contribute to granulomatous inflammation. Humoral mediators coordinate fundamental physiological processes in mammalian macrophages that dictate their functionality during granuloma formation. Interference in humoral pathways, such as complement inhibition, redirects macrophage physiology towards homeostasis and the control of granuloma formation. Exploring the involvement of humoral innate immunity in granuloma formation and the pathogenesis of granulomatous diseases represents a novel research avenue. Emerging evidence highlights a crucial role for humoral innate immunity and macrophage physiology/function in modulating fundamental processes of granuloma formation. Further investigation is necessary to refine the identity of the humoral mediators and cellular mechanisms through which macrophages participate in granuloma formation and maintenance. Research is also warranted to understand how humoral innate immunity is associated with susceptibility to, and clinical courses of granulomatous diseases. The humoral arm of mammalian innate immunity regulates several molecular mechanisms involved in resistance to pathogens, inflammation, and tissue repair. Recent studies highlight the crucial role played by humoral mediators in granulomatous inflammation. However the molecular mechanisms linking the function of these soluble molecules to the initiation and maintenance of granulomas remain elusive. We propose that humoral innate immunity coordinates fundamental physiological processes in macrophages which, in turn, initiate activation and transformation events that enable granuloma formation. We discuss the involvement of humoral mediators in processes such as immune activation, phagocytosis, metabolism, and tissue remodeling, and how these can dictate macrophage functionality during granuloma formation. These advances present opportunities for discovering novel disease factors and developing targeted, more effective treatments for granulomatous diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Intraphagosomal Free Ca 2+ Changes during Phagocytosis.
- Author
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Dewitt, Sharon, Green, Joanna, Laffafian, Iraj, Lewis, Kimberly J., and Hallett, Maurice B.
- Subjects
- *
CALCIUM ions , *PHAGOCYTOSIS , *CELL communication , *CELL membranes , *ENDOCYTOSIS , *CYTOSOL - Abstract
Phagocytosis (and endocytosis) is an unusual cellular process that results in the formation of a novel subcellular organelle, the phagosome. This phagosome contains not only the internalised target of phagocytosis but also the external medium, creating a new border between extracellular and intracellular environments. The boundary at the plasma membrane is, of course, tightly controlled and exploited in ionic cell signalling events. Although there has been much work on the control of phagocytosis by ions, notably, Ca2+ ions influxing across the plasma membrane, increasing our understanding of the mechanism enormously, very little work has been done exploring the phagosome/cytosol boundary. In this paper, we explored the changes in the intra-phagosomal Ca2+ ion content that occur during phagocytosis and phagosome formation in human neutrophils. Measuring Ca2+ ion concentration in the phagosome is potentially prone to artefacts as the intra-phagosomal environment experiences changes in pH and oxidation. However, by excluding such artefacts, we conclude that there are open Ca2+ channels on the phagosome that allow Ca2+ ions to "drain" into the surrounding cytosol. This conclusion was confirmed by monitoring the translocation of the intracellularly expressed YFP-tagged C2 domain of PKC-γ. This approach marked regions of membrane at which Ca2+ influx occurred, the earliest being the phagocytic cup, and then the whole cell. This paper therefore presents data that have novel implications for understanding phagocytic Ca2+ signalling events, such as peri-phagosomal Ca2+ hotspots, and other phenomena. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. The mechanistic basis of the membrane‐permeabilizing activities of the virulence‐associated protein A (VapA) from Rhodococcus equi.
- Author
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Nehls, Christian, Schröder, Marcel, Haubenthal, Thomas, Haas, Albert, and Gutsmann, Thomas
- Subjects
- *
RHODOCOCCUS , *ATOMIC force microscopy , *MEMBRANE lipids , *BILAYER lipid membranes , *PROTEINS , *PHAGOSOMES , *LYSOSOMES - Abstract
Pathogenic Rhodococcus equi release the virulence‐associated protein A (VapA) within macrophage phagosomes. VapA permeabilizes phagosome and lysosome membranes and reduces acidification of both compartments. Using biophysical techniques, we found that VapA interacts with model membranes in four steps: (i) binding, change of mechanical properties, (ii) formation of specific membrane domains, (iii) permeabilization within the domains, and (iv) pH‐specific transformation of domains. Biosensor data revealed that VapA binds to membranes in one step at pH 6.5 and in two steps at pH 4.5 and decreases membrane fluidity. The integration of VapA into lipid monolayers was only significant at lateral pressures <20 mN m−1 indicating preferential incorporation into membrane regions with reduced integrity. Atomic force microscopy of lipid mono‐ and bilayers showed that VapA increased the surface heterogeneity of liquid disordered domains. Furthermore, VapA led to the formation of a new microstructured domain type and, at pH 4.5, to the formation of 5 nm high domains. VapA binding, its integration and lipid domain formation depended on lipid composition, pH, protein concentration and lateral membrane pressure. VapA‐mediated permeabilization is clearly distinct from that caused by classical microbial pore formers and is a key contribution to the multiplication of Rhodococcus equi in phagosomes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Proteomic lung analysis revealed hyper-activation of neutrophil extracellular trap formation in cases of fatal COVID-19
- Author
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Shu Song, Liyan Zeng, Jingjing Xu, Lei Shi, Lingqing Lu, Yun Ling, and Lijun Zhang
- Subjects
COVID-19 ,Proteomics ,Lung ,Bioinformatics ,Neutrophil extracellular trap formation ,Phagosome ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The molecular pathology of lung injury in patients with Corona Virus Disease 2019 (COVID-19) remain unclear. In this study, we performed a proteomic study of lung tissues from seven patients with COVID-19, and eight without. Lung parenchymal tissues with COVID-19 were obtained from autopsy samples, while control samples were obtained from paracancerous tissues. Proteins were extracted using phenol extraction. A tandem mass tag-based quantitative proteomic approach combined with bioinformatic analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues. A total of 6,602, and 6,549 proteins were identified in replicates 1 and 2, respectively. Of these, 307, and 278, respectively, were identified as differentially expressed proteins (DEPs). In total, 216 DEPs were identified in this study. These proteins were enriched in 189 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The downregulated proteins are mainly involved in focal adhesion (n = 5), and the PI3K-Akt signaling pathway (n = 4). The upregulated proteins were related to neutrophil extracellular trap (NET) formation (n = 16), and the phagosome pathway (n = 11). The upregulated proteins in these two pathways interact with one another. Further immunohistochemistry verified NET enrichment in the tissues with COVID-19 compared to the controls. Our results systematically outlined the proteomic profiles of the lung's response to SARS-CoV-2 infection and indicated that NET formation was hyper-activated. These results will hopefully provide new evidence for understanding the mechanism behind fatal COVID-19.
- Published
- 2024
- Full Text
- View/download PDF
6. Phagocytosis-associated genes in Acanthamoeba castellanii feeding on Escherichia coli.
- Author
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Min-Jeong Kim, Eun-Kyung Moon, Hye-Jeong Jo, Fu-Shi Quan, and Hyun-Hee Kong
- Subjects
ACANTHAMOEBA castellanii ,ESCHERICHIA coli ,PHAGOSOMES ,SMALL interfering RNA ,GENE silencing - Abstract
Acanthamoeba species are free-living amoebae those are widely distributed in the environment. They feed on various microorganisms, including bacteria, fungi, and algae. Although majority of the microbes phagocytosed by Acanthamoeba spp. are digested, some pathogenic bacteria thrive within them. Here, we identified the roles of 3 phagocytosis- associated genes (ACA1_077100, ACA1_175060, and AFD36229.1) in A. castellanii. These 3 genes were upregulated after the ingestion of Escherichia coli. However, after the ingestion of Legionella pneumophila, the expression of these 3 genes was not altered after the consumption of L. pneumophila. Furthermore, A. castellanii transfected with small interfering RNS (siRNA) targeting the 3 phagocytosis-associated genes failed to digest phagocytized E. coli. Silencing of ACA1_077100 disabled phagosome formation in the E. coli-ingesting A. castellanii. Alternatively, silencing of ACA1_175060 enabled phagosome formation; however, phagolysosome formation was inhibited. Moreover, suppression of AFD36229.1 expression prevented E. coli digestion and consequently led to the rupturing of A. castellanii. Our results demonstrated that the ACA1_077100, ACA1_175060, and AFD36229.1 genes of Acanthamoeba played crucial roles not only in the formation of phagosome and phagolysosome but also in the digestion of E. coli. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
7. Transcriptomics Meta-Analysis Reveals Phagosome and Innate Immune System Dysfunction as Potential Mechanisms in the Cortex of Alzheimer's Disease Mouse Strains.
- Author
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Widjaya, Michael Anekson, Liu, Chia-Hsin, Lee, Shin-Da, and Cheng, Wei-Chung
- Abstract
Immune-related pathways can affect the immune system directly, such as the chemokine signaling pathway, or indirectly, such as the phagosome pathway. Alzheimer's disease (AD) is reportedly associated with several immune-related pathways. However, exploring its underlying mechanism is challenging in animal studies because AD mouse strains differentially express immune-related pathway characteristics. To overcome this problem, we performed a meta-analysis to identify significant and consistent immune-related AD pathways that are expressed in different AD mouse strains. Next-generation RNA sequencing (RNA-seq) and microarray datasets for the cortex of AD mice from different strains such as APP/PSEN1, APP/PS2, 3xTg, TREM, and 5xFAD were collected from the NCBI GEO database. Each dataset's quality control and normalization were already processed from each original study source using various methods depending on the high-throughput analysis platform (FastQC, median of ratios, RMA, between array normalization). Datasets were analyzed using DESeq2 for RNA-seq and GEO2R for microarray to identify differentially expressed (DE) genes. Significantly DE genes were meta-analyzed using Stouffer's method, with significant genes further analyzed for functional enrichment. Ten datasets representing 20 conditions were obtained from the NCBI GEO database, comprising 116 control and 120 AD samples. The DE analysis identified 284 significant DE genes. The meta-analysis identified three significantly enriched immune-related AD pathways: phagosome, the complement and coagulation cascade, and chemokine signaling. Phagosomes-related genes correlated with complement and immune system. Meanwhile, phagosomes and chemokine signaling genes overlapped with B cells receptors pathway genes indicating potential correlation between phagosome, chemokines, and adaptive immune system as well. The transcriptomic meta-analysis showed that AD is associated with immune-related pathways in the brain's cortex through the phagosome, complement and coagulation cascade, and chemokine signaling pathways. Interestingly, phagosome and chemokine signaling pathways had potential correlation with B cells receptors pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
8. Revisiting the Daily Timing of POS Phagocytosis
- Author
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Paniagua, Antonio E., Sabharwal, Harjas S., Kethu, Kausalya, Chang, Andrew W., Williams, David S., Crusio, Wim E., Series Editor, Dong, Haidong, Series Editor, Radeke, Heinfried H., Series Editor, Rezaei, Nima, Series Editor, Steinlein, Ortrud, Series Editor, Xiao, Junjie, Series Editor, Ash, John D., editor, Pierce, Eric, editor, Anderson, Robert E., editor, Bowes Rickman, Catherine, editor, Hollyfield, Joe G., editor, and Grimm, Christian, editor
- Published
- 2023
- Full Text
- View/download PDF
9. Intraphagosomal Free Ca2+ Changes during Phagocytosis
- Author
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Sharon Dewitt, Joanna Green, Iraj Laffafian, Kimberly J. Lewis, and Maurice B. Hallett
- Subjects
intra-phagosomal Ca2+ ,phagocytosis ,Ca2+ channels ,phagosome ,neutrophil ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Phagocytosis (and endocytosis) is an unusual cellular process that results in the formation of a novel subcellular organelle, the phagosome. This phagosome contains not only the internalised target of phagocytosis but also the external medium, creating a new border between extracellular and intracellular environments. The boundary at the plasma membrane is, of course, tightly controlled and exploited in ionic cell signalling events. Although there has been much work on the control of phagocytosis by ions, notably, Ca2+ ions influxing across the plasma membrane, increasing our understanding of the mechanism enormously, very little work has been done exploring the phagosome/cytosol boundary. In this paper, we explored the changes in the intra-phagosomal Ca2+ ion content that occur during phagocytosis and phagosome formation in human neutrophils. Measuring Ca2+ ion concentration in the phagosome is potentially prone to artefacts as the intra-phagosomal environment experiences changes in pH and oxidation. However, by excluding such artefacts, we conclude that there are open Ca2+ channels on the phagosome that allow Ca2+ ions to “drain” into the surrounding cytosol. This conclusion was confirmed by monitoring the translocation of the intracellularly expressed YFP-tagged C2 domain of PKC-γ. This approach marked regions of membrane at which Ca2+ influx occurred, the earliest being the phagocytic cup, and then the whole cell. This paper therefore presents data that have novel implications for understanding phagocytic Ca2+ signalling events, such as peri-phagosomal Ca2+ hotspots, and other phenomena.
- Published
- 2024
- Full Text
- View/download PDF
10. Proteomics analysis of methionine enkephalin upregulated macrophages against infection by the influenza-A virus
- Author
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Wenrui Fu, Zifeng Xie, Mei Bai, Zhen Zhang, Yuanlong Zhao, and Jing Tian
- Subjects
Macrophage ,MENK ,Proteomics analysis ,Polarization ,Phagosome ,Cytology ,QH573-671 - Abstract
Abstract Macrophages have a vital role in phagocytosis and antiviral effect against invading influenza viruses. Previously, we found that methionine enkephalin (MENK) inhibited influenza virus infection by upregulating the “antiviral state” of macrophages. To investigate the immunoregulatory mechanism of action of MENK on macrophages, we employed proteomic analysis to identify differentially expressed proteins (DEPs) between macrophages infected with the influenza-A virus and cells infected with the influenza-A virus after pretreatment with MENK. A total of 215 DEPs were identified: 164 proteins had upregulated expression and 51 proteins had downregulated expression. Proteomics analysis showed that DEPs were highly enriched in “cytokine-cytokine receptor interaction”, “phagosome”, and “complement and coagulation cascades pathway”. Proteomics analysis revealed that MENK could be an immune modulator or prophylactic for the prevention and treatment of influenza. MENK promoted the polarization of M1 macrophages, activated inflammatory responses, and enhanced phagocytosis and killing function by upregulating opsonizing receptors.
- Published
- 2023
- Full Text
- View/download PDF
11. Respiration supports intraphagosomal filamentation and escape of Candida albicans from macrophages
- Author
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Nicola T. Case, Johannes Westman, Michael T. Hallett, Jonathan Plumb, Aiman Farheen, Michelle E. Maxson, Jessie MacAlpine, Sean D. Liston, Bernhard Hube, Nicole Robbins, Luke Whitesell, Sergio Grinstein, and Leah E. Cowen
- Subjects
hyphae ,morphogenesis ,fungi ,host-pathogen interactions ,phagosome ,glucose ,Microbiology ,QR1-502 - Abstract
ABSTRACTFor the human fungal pathogen Candida albicans, metabolic flexibility and the ability to transition between yeast and filamentous growth states are key virulence traits that enable disease in the host. These traits are particularly important during the interaction of C. albicans with macrophages, where the fungus must utilize multiple alternative carbon sources to survive after being phagocytosed, and filamentation is coupled to fungal escape and immune cell death. Here, we employed functional genomic screening of conditional-expression mutants covering >50% of the C. albicans genome to identify genes selectively required for filamentation inside macrophages. Through manual and machine learning-based image analyses, we uncovered a role for the mitochondrial ribosome, respiration, and the SNF1 AMP-activated kinase complex in governing filamentous growth within the phagosome, suggesting that C. albicans relies on respiration to evade the antifungal activities of macrophages. We demonstrate that downregulating the expression of these genes reduces ATP levels and impedes filamentation as well as growth under monoculture conditions in medium lacking glucose. In co-culture with physiological glucose concentration, downregulation of genes involved in mitochondrial function and respiration prevented C. albicans from expanding within the phagosome, escaping, and inducing immune cell death. Together, our work provides new insights into the impact of metabolism on the interaction between C. albicans and macrophages, highlighting respiration and the SNF1 AMP-activated kinase as key effectors of C. albicans metabolic flexibility and filamentation within phagocytes.IMPORTANCECandida albicans is a leading human fungal pathogen that often causes life-threatening infections in immunocompromised individuals. The ability of C. albicans to transition between yeast and filamentous forms is key to its virulence, and this occurs in response to many host-relevant cues, including engulfment by host macrophages. While previous efforts identified C. albicans genes required for filamentation in other conditions, the genes important for this morphological transition upon internalization by macrophages remained largely enigmatic. Here, we employed a functional genomic approach to identify genes that enable C. albicans filamentation within macrophages and uncovered a role for the mitochondrial ribosome, respiration, and the SNF1 AMP-activated kinase complex. Additionally, we showed that glucose uptake and glycolysis by macrophages support C. albicans filamentation. This work provides insights into the metabolic dueling that occurs during the interaction of C. albicans with macrophages and identifies vulnerabilities in C. albicans that could serve as promising therapeutic targets.
- Published
- 2023
- Full Text
- View/download PDF
12. Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species.
- Author
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Fantone, Kayla M., Goldberg, Joanna B., Stecenko, Arlene A., and Rada, Balázs
- Subjects
NEUTROPHILS ,METHICILLIN-resistant staphylococcus aureus ,REACTIVE oxygen species ,CYSTIC fibrosis ,SPUTUM ,LEUCOCYTE elastase - Abstract
Cystic fibrosis (CF) airway disease is characterized by chronic polymicrobial infections and an infiltration of neutrophils (PMNs). Staphylococcus aureus has been the most prevalent respiratory pathogen in CF. In particular, methicillin-resistant S. aureus (MRSA) represents a huge clinical burden in CF due to its association with lung disease and increased resistance to antibiotics. In CF, PMNs are unable to kill and clear MRSA. The reason for this remains largely unknown. Our study found that CF PMNs are as equally capable of killing MRSA as healthy PMNs. We show that the CF sputum, however, significantly impairs the ability of human PMNs to kill CF MRSA isolates. In the absence of CF sputum, PMNs kill MRSA via intracellular mechanisms mediated by phagocytosis, rather than extracellular mechanisms via NET formation. CF sputum does not affect the phagocytosis of MRSA via healthy or CF PMNs. Our results demonstrate that CF sputum exposure impairs phagosomal levels of reactive oxygen species (ROS) in MRSA-phagocytosing PMNs. While phagosomal co-localizations of MRSA with primary granule markers, myeloperoxidase and cathepsin D, were significantly reduced upon CF sputum exposure, that of a third azurophilic granule marker, neutrophil elastase, remained unaffected. This suggests that CF sputum does not compromise the fusion of primary granules with phagosomes but diminishes phagosomal ROS levels via another, likely more specific, mechanism. Overall, we identified the airway environment as an important factor that restricts neutrophils' oxidative microbicidal activities in CF against MRSA. These results deliver new details of the complex host–pathogen interactions present in the CF lung. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Bi-functional particles for real-time phagosome acidification and proteolysis multiplex assay in macrophages.
- Author
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Méndez-Alejandre, Alba, Raymond, Benjamin Bernard Armando, Trost, Matthias, and Luis Marín-Rubio, José
- Subjects
ACIDIFICATION ,PROTEOLYSIS ,MACROPHAGES ,BIOLOGICAL assay ,IMMUNE response - Abstract
Phagosome acidification and proteolysis are essential processes in the immune response to contain and eliminate pathogens. In recent years, there has been an increased desire for a rapid and accurate method of assessing these processes in real-time. Here, we outline the development of a multiplexed assay that allows simultaneous monitoring of phagosome acidification and proteolysis in the same sample using silica beads conjugated to pHrodo and DQ BSA. We describe in detail how to prepare the bi-functional particles and show proof of concept using differentially activated macrophages. This multiplexed spectrophotometric assay allows rapid and accurate assessment of phagosome acidification and proteolysis in real-time and could provide valuable information for understanding the immune response to pathogen invasion. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
14. Role of BCR and FNBP1 Proteins in Phagocytosis as a Model of Membrane Rearrangements with Chronic Myelogenous Leukemia.
- Author
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Antonenko, S. V., Gurianov, D. S., Kravchuk, I. V., Dybkov, M. V., Shvachko, L. P., and Telegeev, G. D.
- Abstract
Chronic myelogenous leukemia (CML) is a myeloproliferative neoplasm resulting from the emergence of abnormal hematopoietic stem cells carrying a Bcr-Abl oncoprotein as a result of a reciprocal translocation between the chromosomes 9 and 22. The main elements of the disease's pathogenesis are caused by both increased tyrosine kinase activity of the Abl protein and the role of a Bcr part of the hybrid protein. The presence of PH domain in the Bcr determines its interaction with PI(3)P of the phagosomal membrane. We demonstrated that this interaction is accompanied by a colocalization of the Bcr with the FNBPI protein in the phagosomes of J774 macrophage cells. A model of the effect of the Bcr-Abl oncoprotein on the formation of the ROS excess with CML due to uncontrolled expression of phagosomal NADP oxidase is presented. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. Bi-functional particles for real-time phagosome acidification and proteolysis multiplex assay in macrophages
- Author
-
Alba Méndez-Alejandre, Benjamin Bernard Armando Raymond, Matthias Trost, and José Luis Marín-Rubio
- Subjects
phagosome ,proteolysis ,acidification ,macrophages ,bacteria ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Phagosome acidification and proteolysis are essential processes in the immune response to contain and eliminate pathogens. In recent years, there has been an increased desire for a rapid and accurate method of assessing these processes in real-time. Here, we outline the development of a multiplexed assay that allows simultaneous monitoring of phagosome acidification and proteolysis in the same sample using silica beads conjugated to pHrodo and DQ BSA. We describe in detail how to prepare the bi-functional particles and show proof of concept using differentially activated macrophages. This multiplexed spectrophotometric assay allows rapid and accurate assessment of phagosome acidification and proteolysis in real-time and could provide valuable information for understanding the immune response to pathogen invasion.
- Published
- 2023
- Full Text
- View/download PDF
16. Rab GTPase regulation of phagosome--lysosome fusion is bypassed in the presence of micromolar Ca2+.
- Author
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Becker, Julia, Schleinitz, Ariane, Hermsen, Christina, Rappold, Sabrina, Saftig, Paul, Jeschke, Andreas, and Haas, Albert
- Subjects
- *
GUANOSINE triphosphatase , *ANNEXINS , *NUCLEAR fusion , *MEMBRANE fusion , *PROTEIN receptors , *CELL fusion , *MERGERS & acquisitions - Abstract
Several ATP- and cytosol-dependent fusion processes between membranes of the endocytic and exocytic pathways have been biochemically reconstituted. Here, we present a phagosome-lysosome fusion reaction that is driven by micromolar concentrations of Ca2+ in the absence of ATP and cytosol. Investigating classical fusion and Ca2+-driven fusion (CaFu) side-by-side in vitro, using the same membrane preparations, we show that CaFu is faster than standard fusion (StaFu), leads to larger fusion products and is not blocked by established inhibitors of StaFu. A Ca2+ concentration of ~120µM supports maximal membrane attachment, and 15µM Ca2+ supports maximal membrane fusion, indicating that Ca2+ has both a membrane-binding activity and a fusion-promoting activity. StaFu and CaFu are inhibited by a mutant form of α-SNAP (NAPA) that does not support soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) activation, and both are inhibited by a mixture of the cytosolic domains of three cognate Q-SNARE proteins, demonstrating a role of SNAREs in Ca2+-driven membrane merger. CaFu is independent of the Ca2+-regulated proteins synaptotagmin-7, calmodulin, and annexins A2 and A7. We propose that CaFu corresponds to the last step of phagosome-lysosome fusion, when a raised Ca2+ concentration from the compartment lumen activates SNAREs for fusion. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
17. Rab GTPase regulation of phagosome--lysosome fusion is bypassed in the presence of micromolar Ca2+.
- Author
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Becker, Julia, Schleinitz, Ariane, Hermsen, Christina, Rappold, Sabrina, Saftig, Paul, Jeschke, Andreas, and Haas, Albert
- Subjects
GUANOSINE triphosphatase ,ANNEXINS ,NUCLEAR fusion ,MEMBRANE fusion ,PROTEIN receptors ,CELL fusion ,MERGERS & acquisitions - Abstract
Several ATP- and cytosol-dependent fusion processes between membranes of the endocytic and exocytic pathways have been biochemically reconstituted. Here, we present a phagosome-lysosome fusion reaction that is driven by micromolar concentrations of Ca
2+ in the absence of ATP and cytosol. Investigating classical fusion and Ca2+ -driven fusion (CaFu) side-by-side in vitro, using the same membrane preparations, we show that CaFu is faster than standard fusion (StaFu), leads to larger fusion products and is not blocked by established inhibitors of StaFu. A Ca2+ concentration of ~120µM supports maximal membrane attachment, and 15µM Ca2+ supports maximal membrane fusion, indicating that Ca2+ has both a membrane-binding activity and a fusion-promoting activity. StaFu and CaFu are inhibited by a mutant form of α-SNAP (NAPA) that does not support soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) activation, and both are inhibited by a mixture of the cytosolic domains of three cognate Q-SNARE proteins, demonstrating a role of SNAREs in Ca2+ -driven membrane merger. CaFu is independent of the Ca2+-regulated proteins synaptotagmin-7, calmodulin, and annexins A2 and A7. We propose that CaFu corresponds to the last step of phagosome-lysosome fusion, when a raised Ca2+ concentration from the compartment lumen activates SNAREs for fusion. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
18. Proteomics analysis of methionine enkephalin upregulated macrophages against infection by the influenza-A virus.
- Author
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Fu, Wenrui, Xie, Zifeng, Bai, Mei, Zhang, Zhen, Zhao, Yuanlong, and Tian, Jing
- Subjects
- *
VIRUS diseases , *PROTEOMICS , *MACROPHAGES , *METHIONINE , *PROTEIN expression - Abstract
Macrophages have a vital role in phagocytosis and antiviral effect against invading influenza viruses. Previously, we found that methionine enkephalin (MENK) inhibited influenza virus infection by upregulating the "antiviral state" of macrophages. To investigate the immunoregulatory mechanism of action of MENK on macrophages, we employed proteomic analysis to identify differentially expressed proteins (DEPs) between macrophages infected with the influenza-A virus and cells infected with the influenza-A virus after pretreatment with MENK. A total of 215 DEPs were identified: 164 proteins had upregulated expression and 51 proteins had downregulated expression. Proteomics analysis showed that DEPs were highly enriched in "cytokine-cytokine receptor interaction", "phagosome", and "complement and coagulation cascades pathway". Proteomics analysis revealed that MENK could be an immune modulator or prophylactic for the prevention and treatment of influenza. MENK promoted the polarization of M1 macrophages, activated inflammatory responses, and enhanced phagocytosis and killing function by upregulating opsonizing receptors. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. Vomocytosis: Too Much Booze, Base, or Calcium?
- Author
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Cruz-Acuña, Melissa, Pacifici, Noah, and Lewis, Jamal S
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Biological Sciences ,Immunology ,Infectious Diseases ,HIV/AIDS ,Sexually Transmitted Infections ,2.1 Biological and endogenous factors ,Infection ,Animals ,Calcium ,Cryptococcus neoformans ,Humans ,Hydrogen-Ion Concentration ,Macrophages ,Mice ,Phagocytosis ,Phagosomes ,Phenotype ,vomocytosis ,macrophage ,pH ,phagosome ,nonlytic exocytosis ,Microbiology ,Biochemistry and cell biology ,Medical microbiology - Abstract
Macrophages are well known for their phagocytic activity and their role in innate immune responses. Macrophages eat non-self particles, via a variety of mechanisms, and typically break down internalized cargo into small macromolecules. However, some pathogenic agents have the ability to evade this endosomal degradation through a nonlytic exocytosis process termed vomocytosis. This phenomenon has been most often studied for Cryptococcus neoformans, a yeast that causes roughly 180,000 deaths per year, primarily in immunocompromised (e.g., human immunodeficiency virus [HIV]) patients. Existing dogma purports that vomocytosis involves distinctive cellular pathways and intracellular physicochemical cues in the host cell during phagosomal maturation. Moreover, it has been observed that the immunological state of the individual and macrophage phenotype affect vomocytosis outcomes. Here we compile the current knowledge on the factors (with respect to the phagocytic cell) that promote vomocytosis of C. neoformans from macrophages.
- Published
- 2019
20. The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat
- Author
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Guo-Dong He, Xiao-Cong Liu, Xing-Hua Hou, and Ying-Qing Feng
- Subjects
Trimethylamine N-oxide ,visceral white adipose tissue ,hypertension ,fatty acids ,phagosome ,lysosome ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Cytology ,QH573-671 ,Physiology ,QP1-981 - Abstract
Strong links have been reported among trimethylamine N-oxide (TMAO), visceral white adipose tissue (vWAT), and cardiometabolic diseases. However, the effects of TMAO on vWAT in hypertension remained incompletely explored. The impact of a chronic 22-week-long treatment with 1 g/L TMAO on vWAT, and its transcriptional and metabolic changes in spontaneously hypertensive rats (SHRs) were evaluated by serum cytokine measurements, histological analysis, fatty acid determinations, and co-expression network analyses. TMAO increased the serum interleukin-6 levels and insulin secretion in SHRs. The adipocyte size was diminished in the SHR 1 g/L TMAO group. In addition, one kind of monounsaturated fatty acids (cis-15-tetracosenoate) and four kinds of polyunsaturated fatty acids (cis-11,14,17-eicosatrienoic acid, docosatetraenoate, docosapentaenoate n-3, and docosapentaenoate n-6) were elevated by TMAO treatment. Three co-expression modules significantly related to TMAO treatment were identified and pathway enrichment analyses indicated that phagosome, lysosome, fatty acid metabolism, valine, leucine, and isoleucine degradation and metabolic pathways were the most significantly altered biological pathways. This study shed new light on the metabolic roles of TMAO on the vWAT of SHRs. TMAO regulated the metabolic status of vWAT, including reduced lipogenesis and an improved specific fatty acid composition. The mechanisms underlying these effects likely involve phagosome and lysosome pathways.
- Published
- 2022
- Full Text
- View/download PDF
21. The formation and function of the neutrophil phagosome.
- Author
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Naish, Emily, Wood, Alexander JT, Stewart, Andrew P, Routledge, Matthew, Morris, Andrew Conway, Chilvers, Edwin R, and Lodge, Katharine M
- Subjects
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NICOTINAMIDE adenine dinucleotide phosphate , *NEUTROPHILS , *REACTIVE oxygen species , *PHAGOCYTOSIS - Abstract
Summary: Neutrophils are the most abundant circulating leukocyte and are crucial to the initial innate immune response to infection. One of their key pathogen‐eliminating mechanisms is phagocytosis, the process of particle engulfment into a vacuole‐like structure called the phagosome. The antimicrobial activity of the phagocytic process results from a collaboration of multiple systems and mechanisms within this organelle, where a complex interplay of ion fluxes, pH, reactive oxygen species, and antimicrobial proteins creates a dynamic antimicrobial environment. This complexity, combined with the difficulties of studying neutrophils ex vivo, has led to gaps in our knowledge of how the neutrophil phagosome optimizes pathogen killing. In particular, controversy has arisen regarding the relative contribution and integration of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase‐derived antimicrobial agents and granule‐delivered antimicrobial proteins. Clinical syndromes arising from dysfunction in these systems in humans allow useful insight into these mechanisms, but their redundancy and synergy add to the complexity. In this article, we review the current knowledge regarding the formation and function of the neutrophil phagosome, examine new insights into the phagosomal environment that have been permitted by technological advances in recent years, and discuss aspects of the phagocytic process that are still under debate. [ABSTRACT FROM AUTHOR]
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- 2023
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22. Inside the phagosome: A bacterial perspective.
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Hampton, Mark B. and Dickerhof, Nina
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BACTERIAL genes , *PHAGOSOMES , *BIOCHEMISTRY , *NEUTROPHILS - Abstract
Summary: The neutrophil phagosome is one of the most hostile environments that bacteria must face and overcome if they are to succeed as pathogens. Targeting bacterial defense mechanisms should lead to new therapies that assist neutrophils to kill pathogens, but this has not yet come to fruition. One of the limiting factors in this effort has been our incomplete knowledge of the complex biochemistry that occurs within the rapidly changing environment of the phagosome. The same compartmentalization that protects host tissue also limits our ability to measure events within the phagosome. In this review, we highlight the limitations in our knowledge, and how the contribution of bacteria to the phagosomal environment is often ignored. There appears to be significant heterogeneity among phagosomes, and it is important to determine whether survivors have more efficient defenses or whether they are ingested into less threatening environments than other bacteria. As part of these efforts, we discuss how monitoring or recovering bacteria from phagosomes can provide insight into the conditions they have faced. We also encourage the use of unbiased screening approaches to identify bacterial genes that are essential for survival inside neutrophil phagosomes. [ABSTRACT FROM AUTHOR]
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- 2023
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23. Specific preadaptations of Rhodococcus equi cooperate with its Virulence‐associated protein A during macrophage infection.
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Haubenthal, Thomas, Hansen, Philipp, Krämer, Ina, Gindt, Mélanie, Jünger‐Leif, Alexandra, Utermöhlen, Olaf, and Haas, Albert
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RHODOCOCCUS , *MACROPHAGES , *BACTERIAL proteins , *VACCINE development , *INFECTION , *PLANT growth , *FOALS - Abstract
Gram‐positive Rhodococcus equi (Prescotella equi) is a lung pathogen of foals and immunocompromised humans. Intra‐macrophage multiplication requires production of the bacterial Virulence‐associated protein A (VapA) which is released into the phagosome lumen. VapA pH‐neutralizes intracellular compartments allowing R. equi to multiply in an atypical macrophage phagolysosome. Here, we show that VapA does not support intra‐macrophage growth of several other bacterial species demonstrating that only few bacteria have the specific preadaptations needed to profit from VapA. We show that the closest relative of R. equi, environmental Rhodococcus defluvii (Prescotella defluvii), does not multiply in macrophages at 37°C even when VapA is present because of its thermosensitivity but it does so once the infection temperature is lowered providing rare experimental evidence for 'thermal restriction'. Using growth experiments with isolated macrophage lysosomes and modified infection schemes we provide evidence that R. equi resists the attack by phagolysosome contents at low pH for several hours. During this time, R. equi produces and secretes VapA which enables it to grow at the expense of lysosome constituents. We present arguments that, under natural infection conditions, R. equi is VapA‐less during the initial encounter with the host. This has important implications for vaccine development. [ABSTRACT FROM AUTHOR]
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- 2023
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24. Immunopeptidomics reveals determinants of Mycobacterium tuberculosis antigen presentation on MHC class I
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Owen Leddy, Forest M White, and Bryan D Bryson
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m. tuberculosis ,antigen presentation ,MHC-I ,secretion systems ,phagosome ,macrophages ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
CD8+ T cell recognition of Mycobacterium tuberculosis (Mtb)-specific peptides presented on major histocompatibility complex class I (MHC-I) contributes to immunity to tuberculosis (TB), but the principles that govern presentation of Mtb antigens on MHC-I are incompletely understood. In this study, mass spectrometry (MS) analysis of the MHC-I repertoire of Mtb-infected primary human macrophages reveals that substrates of Mtb’s type VII secretion systems (T7SS) are overrepresented among Mtb-derived peptides presented on MHC-I. Quantitative, targeted MS shows that ESX-1 activity is required for presentation of Mtb peptides derived from both ESX-1 substrates and ESX-5 substrates on MHC-I, consistent with a model in which proteins secreted by multiple T7SSs access a cytosolic antigen processing pathway via ESX-1-mediated phagosome permeabilization. Chemical inhibition of proteasome activity, lysosomal acidification, or cysteine cathepsin activity did not block presentation of Mtb antigens on MHC-I, suggesting involvement of other proteolytic pathways or redundancy among multiple pathways. Our study identifies Mtb antigens presented on MHC-I that could serve as targets for TB vaccines, and reveals how the activity of multiple T7SSs interacts to contribute to presentation of Mtb antigens on MHC-I.
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- 2023
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25. Lipid droplets as multifunctional organelles related to the mechanism of evasion during mycobacterial infection
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Patrícia Elaine de Almeida, Núbia Maria Pereira de Sousa, Pollianne Garbero Rampinelli, Renata Vieira de Sousa Silva, José Raimundo Correa, and Heloisa D’Avila
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lipid metabolism ,lipid droplets ,mycobacteria ,tuberculosis ,phagosome ,Microbiology ,QR1-502 - Abstract
Tuberculosis (TB) is an infectious disease caused by the bacteria of the Mycobaterium tuberculosis (Mtb) complex. The modulation of the lipid metabolism has been implicated in the immune response regulation, including the formation of lipid droplets (LD)s, LD-phagosome association and eicosanoid synthesis. Mtb, M. bovis BCG and other pathogenic mycobacteria, as well as wall components, such as LAM, can induce LDs formation in a mechanism involving surface receptors, for instance TLRs, CD36, CD14, CD11b/CD18 and others. In addition, the activation of the lipid-activated nuclear receptor PPARγ is involved in the mechanisms of LD biogenesis, as well as in the modulation of the synthesis of lipid mediators. In infected cells, LDs are sites of compartmentalized prostaglandin E2 synthesis involved in macrophage deactivation, bacterial replication and regulation of the host cytokine profile. LDs also have a function in vesicle traffic during infection. Rab7 and RILP, but not Rab5, are located on LDs of infected macrophages, suggesting that LDs and phagosomes could exchange essential proteins for phagosomal maturation, interfering in mycobacterial survival. The pharmacological inhibition of LDs biogenesis affects the bacterial replication and the synthesis of lipid mediators and cytokines, suggesting that LDs may be new targets for antimicrobial therapies. However, it is still controversial if the accumulation of LDs favors the mycobacterial survival acting as an escape mechanism, or promotes the host resistance to infection. Thus, in this mini-review we discuss recent advances in understanding the important role of LDs in the course of infections and the implications for the pathophysiology of mycobacteriosis.
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- 2023
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26. The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat.
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He, Guo-Dong, Liu, Xiao-Cong, Hou, Xing-Hua, and Feng, Ying-Qing
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WHITE adipose tissue , *TRIMETHYLAMINE , *MONOUNSATURATED fatty acids , *UNSATURATED fatty acids , *METABOLISM - Abstract
Strong links have been reported among trimethylamine N-oxide (TMAO), visceral white adipose tissue (vWAT), and cardiometabolic diseases. However, the effects of TMAO on vWAT in hypertension remained incompletely explored. The impact of a chronic 22-week-long treatment with 1 g/L TMAO on vWAT, and its transcriptional and metabolic changes in spontaneously hypertensive rats (SHRs) were evaluated by serum cytokine measurements, histological analysis, fatty acid determinations, and co-expression network analyses. TMAO increased the serum interleukin-6 levels and insulin secretion in SHRs. The adipocyte size was diminished in the SHR 1 g/L TMAO group. In addition, one kind of monounsaturated fatty acids (cis-15-tetracosenoate) and four kinds of polyunsaturated fatty acids (cis-11,14,17-eicosatrienoic acid, docosatetraenoate, docosapentaenoate n-3, and docosapentaenoate n-6) were elevated by TMAO treatment. Three co-expression modules significantly related to TMAO treatment were identified and pathway enrichment analyses indicated that phagosome, lysosome, fatty acid metabolism, valine, leucine, and isoleucine degradation and metabolic pathways were the most significantly altered biological pathways. This study shed new light on the metabolic roles of TMAO on the vWAT of SHRs. TMAO regulated the metabolic status of vWAT, including reduced lipogenesis and an improved specific fatty acid composition. The mechanisms underlying these effects likely involve phagosome and lysosome pathways. [ABSTRACT FROM AUTHOR]
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- 2022
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27. The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infection.
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Bilkei‐Gorzo, Orsolya, Heunis, Tiaan, Marín‐Rubio, José Luis, Cianfanelli, Francesca Romana, Raymond, Benjamin Bernard Armando, Inns, Joseph, Fabrikova, Daniela, Peltier, Julien, Oakley, Fiona, Schmid, Ralf, Härtlova, Anetta, and Trost, Matthias
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BACTERIAL diseases , *UBIQUITIN ligases , *KNOCKOUT mice , *INTERFERON gamma , *UBIQUITIN , *NATURAL immunity , *UBIQUITINATION - Abstract
Phagocytosis is a key process in innate immunity and homeostasis. After particle uptake, newly formed phagosomes mature by acquisition of endolysosomal enzymes. Macrophage activation by interferon gamma (IFN‐γ) increases microbicidal activity, but delays phagosomal maturation by an unknown mechanism. Using quantitative proteomics, we show that phagosomal proteins harbour high levels of typical and atypical ubiquitin chain types. Moreover, phagosomal ubiquitylation of vesicle trafficking proteins is substantially enhanced upon IFN‐γ activation of macrophages, suggesting a role in regulating phagosomal functions. We identified the E3 ubiquitin ligase RNF115, which is enriched on phagosomes of IFN‐γ activated macrophages, as an important regulator of phagosomal maturation. Loss of RNF115 protein or ligase activity enhanced phagosomal maturation and increased cytokine responses to bacterial infection, suggesting that both innate immune signalling from the phagosome and phagolysosomal trafficking are controlled through ubiquitylation. RNF115 knock‐out mice show less tissue damage in response to S. aureus infection, indicating a role of RNF115 in inflammatory responses in vivo. In conclusion, RNF115 and phagosomal ubiquitylation are important regulators of innate immune functions during bacterial infections. Synopsis: Interferon gamma activation of macrophages increases phagocytic uptake of particles, but delays phagosomal maturation via unclear mechanisms. Here, a phagosome‐localised E3 ubiquitin ligase, RNF115, is shown to control phagosomal maturation and cytokine responses to bacterial infection in vitro and in vivo. Quantitative proteomics reveal high levels of typical and atypical ubiquitin chain types on phagosomal proteins.RNF115 is enriched on phagosomes and ubiquitylates various proteins involved in immune responses and vesicle trafficking.RNF115 regulates Salmonella adaptation and replication within macrophages.Loss of RNF115 increases inflammatory responses in vitro and reduces tissue damage to infection in knock‐out mice. [ABSTRACT FROM AUTHOR]
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- 2022
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28. Cell-Level Analysis Visualizing Photodynamic Therapy with Porphylipoprotein and Talaporphyrin Sodium.
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Kamiyanagi, Mayuka, Taninaka, Atsushi, Ugajin, Shunta, Nagoshi, Yu, Kurokawa, Hiromi, Ochiai, Takahiro, Arashida, Yusuke, Takeuchi, Osamu, Matsui, Hirofumi, and Shigekawa, Hidemi
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PHOTODYNAMIC therapy , *REACTIVE oxygen species , *SODIUM , *PHAGOSOMES , *ELASTIC modulus - Abstract
We revealed the difference in the mechanism of photodynamic therapy (PDT) between two photosensitizers: porphylipoprotein (PLP), which has recently attracted attention for its potential to be highly effective in treating cancer, and talaporphyrin sodium (NPe6). (1) NPe6 accumulates in lysosomes, whereas PLP is incorporated into phagosomes formed by PLP injection. (2) PDT causes NPe6 to generate reactive oxygen species, thereby producing actin filaments and stress fibers. In the case of PLP, however, reactive oxygen species generated by PDT remain in the phagosomes until the phagosomal membrane is destroyed, which delays the initiation of RhoA activation and RhoA*/ROCK generation. (4) After the disruption of the phagosomal membrane, however, the outflow of various reactive oxygen species accelerates the production of actin filaments and stress fibers, and blebbing occurs earlier than in the case of NPe6. (5) PLP increases the elastic modulus of cells without RhoA activity in the early stage. This is because phagosomes are involved in polymerizing actin filaments and pseudopodia formation. Considering the high selectivity and uptake of PLP into cancer cells, a larger effect with PDT can be expected by skillfully combining the newly discovered characteristics, such as the appearance of a strong effect at an early stage. [ABSTRACT FROM AUTHOR]
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- 2022
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29. Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes.
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Wogram, Emile, Sümpelmann, Felix, Dong, Wentao, Rawat, Eshaan, Fernández Maestre, Inés, Fu, Dongdong, Braswell, Brandyn, Khalil, Andrew, Buescher, Joerg M., Mittler, Gerhard, Borner, Georg H.H., Vlachos, Andreas, Tholen, Stefan, Schilling, Oliver, Bell, George W., Rambold, Angelika S., Akhtar, Asifa, Schnell, Oliver, Beck, Jürgen, and Abu-Remaileh, Monther
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HOMEOSTASIS , *PLURIPOTENT stem cells , *HUMAN stem cells , *QUINOLINIC acid , *PHAGOSOMES - Abstract
Microglia are the resident macrophages of the central nervous system (CNS). Their phagocytic activity is central during brain development and homeostasis—and in a plethora of brain pathologies. However, little is known about the composition, dynamics, and function of human microglial phagosomes under homeostatic and pathological conditions. Here, we developed a method for rapid isolation of pure and intact phagosomes from human pluripotent stem cell-derived microglia under various in vitro conditions, and from human brain biopsies, for unbiased multiomic analysis. Phagosome profiling revealed that microglial phagosomes were equipped to sense minute changes in their environment and were highly dynamic. We detected proteins involved in synapse homeostasis, or implicated in brain pathologies, and identified the phagosome as the site where quinolinic acid was stored and metabolized for de novo nicotinamide adenine dinucleotide (NAD+) generation in the cytoplasm. Our findings highlight the central role of phagosomes in microglial functioning in the healthy and diseased brain. [Display omitted] • Method development for rapid isolation of human microglial phagosomes • Methodology allows for unbiased multiomic profiling in vitro and ex vivo • Microglial phagosomes contain presynaptic rather than postsynaptic proteins • Microglial de novo NAD+ metabolism goes through the phagosome Little is known about the composition, dynamics, and function of human microglial phagosomes under homeostatic and pathological conditions. Wogram et al. develop a method to isolate human microglial phagosomes for unbiased metabolomic and proteomic profiling in vitro and ex vivo. They identify a dynamic phagosomal proteome and significant enrichment of presynaptic proteins and quinolinic acid. [ABSTRACT FROM AUTHOR]
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- 2024
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30. Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species
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Kayla M. Fantone, Joanna B. Goldberg, Arlene A. Stecenko, and Balázs Rada
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cystic fibrosis ,PMN ,MRSA ,phagocytosis ,sputum ,phagosome ,Medicine - Abstract
Cystic fibrosis (CF) airway disease is characterized by chronic polymicrobial infections and an infiltration of neutrophils (PMNs). Staphylococcus aureus has been the most prevalent respiratory pathogen in CF. In particular, methicillin-resistant S. aureus (MRSA) represents a huge clinical burden in CF due to its association with lung disease and increased resistance to antibiotics. In CF, PMNs are unable to kill and clear MRSA. The reason for this remains largely unknown. Our study found that CF PMNs are as equally capable of killing MRSA as healthy PMNs. We show that the CF sputum, however, significantly impairs the ability of human PMNs to kill CF MRSA isolates. In the absence of CF sputum, PMNs kill MRSA via intracellular mechanisms mediated by phagocytosis, rather than extracellular mechanisms via NET formation. CF sputum does not affect the phagocytosis of MRSA via healthy or CF PMNs. Our results demonstrate that CF sputum exposure impairs phagosomal levels of reactive oxygen species (ROS) in MRSA-phagocytosing PMNs. While phagosomal co-localizations of MRSA with primary granule markers, myeloperoxidase and cathepsin D, were significantly reduced upon CF sputum exposure, that of a third azurophilic granule marker, neutrophil elastase, remained unaffected. This suggests that CF sputum does not compromise the fusion of primary granules with phagosomes but diminishes phagosomal ROS levels via another, likely more specific, mechanism. Overall, we identified the airway environment as an important factor that restricts neutrophils’ oxidative microbicidal activities in CF against MRSA. These results deliver new details of the complex host–pathogen interactions present in the CF lung.
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- 2023
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31. Endoplasmic reticulum—Phagosome contact sites from the cradle to the grave
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Mahlegha Ghavami and Gregory D. Fairn
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phagocytosis ,phagosome ,calcium ,cholesterol ,ER contact sites ,ORP1L ,Biology (General) ,QH301-705.5 - Abstract
Phagocytosis is a key component of the innate immune system used to ingest apoptotic cells and microorganisms for their destruction and recycling of macromolecules and the presentation of antigens to adaptive immune system cells. The newly formed vacuole or nascent phagosome undergoes a maturation process reminiscent of the classical endocytic maturation process, reaching a highly degradative phagolysosome stage before its tubulovesicular breakdown into lysosomes. The process is highly regulated and can be disrupted by various pathogenic organisms. The exchange of proteins, lipids, and other metabolites between organelles, including maturing phagosomes, is enabled by two processes, vesicular and non-vesicular transport at membrane contact sites (MCS). For decades the specific role(s) of the endoplasmic reticulum (ER) in phagocytosis has been the subject of much debate. In parallel, the last two decades have seen a burst in research on the numerous roles of ER contact sites and resident proteins in all aspects of organelle biology. Here, in this minireview, we describe ER-phagosome contact sites’ functions from the early stages of particle engulfment to the phagolysosome dissolution into lysosomes. We also discuss several aspects of ER–phagosome contact sites that remain to be explored.
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- 2022
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32. Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasite Entamoeba histolytica
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Kumiko Nakada-Tsukui, Natsuki Watanabe, Kumiko Shibata, Ratna Wahyuni, Eri Miyamoto, and Tomoyoshi Nozaki
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autophagy ,Atg8 ,Entamoeba histolytica ,phagosome ,proteome ,gene silencing ,Microbiology ,QR1-502 - Abstract
Autophagy is one of the bulk degradation systems and is conserved throughout eukaryotes. In the enteric protozoan parasite Entamoeba histolytica, the causative agent of human amebiasis, Atg8 is not exclusively involved in autophagy per se but also in other membrane traffic-related pathways such as phagosome biogenesis. We previously reported that repression of atg8 gene expression by antisense small RNA-mediated transcriptional gene silencing (gs) resulted in growth retardation, delayed endocytosis, and reduced acidification of endosomes and phagosomes. In this study, to better understand the role of Atg8 in phagocytosis and trogocytosis, we conducted a comparative proteomic analysis of phagosomes isolated from wild type and atg8-gs strains. We found that 127 and 107 proteins were detected >1.5-fold less or more abundantly, respectively, in phagosomes isolated from the atg8-gs strain, compared to the control strain. Among 127 proteins whose abundance was reduced in phagosomes from atg8-gs, a panel of proteins related to fatty acid metabolism, phagocytosis, and endoplasmic reticulum (ER) homeostasis was identified. Various lysosomal hydrolases and their receptors also tend to be excluded from phagosomes by atg8-gs, reinforcing the notion that Atg8 is involved in phagosomal acidification and digestion. On the contrary, among 107 proteins whose abundance increased in phagosomes from atg8-gs strain, ribosome-related proteins and metabolite interconversion enzymes are enriched. We further investigated the localization of several representative proteins, including adenylyl cyclase-associated protein and plasma membrane calcium pump, both of which were demonstrated to be recruited to phagosomes and trogosomes via an Atg8-dependent mechanism. Taken together, our study has provided the basis of the phagosome proteome to further elucidate molecular events in the Atg8-dependent regulatory network of phagosome/trogosome biogenesis in E. histolytica.
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- 2022
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33. Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss
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Hernandez, Michael X, Jiang, Shan, Cole, Tracy A, Chu, Shu-Hui, Fonseca, Maria I, Fang, Melody J, Hohsfield, Lindsay A, Torres, Maria D, Green, Kim N, Wetsel, Rick A, Mortazavi, Ali, and Tenner, Andrea J
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Biological Sciences ,Genetics ,Neurodegenerative ,Neurosciences ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Dementia ,Aging ,Behavioral and Social Science ,Acquired Cognitive Impairment ,Brain Disorders ,Alzheimer's Disease ,Basic Behavioral and Social Science ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Alzheimer Disease ,Animals ,Cognition ,Hippocampus ,Humans ,Inflammation ,Mice ,Mice ,Knockout ,Microglia ,Receptor ,Anaphylatoxin C5a ,Signal Transduction ,C5a ,C5a receptor ,Alzheimer's disease ,Complement ,Mouse models ,Behavior ,Gene expression ,Phagosome ,Alzheimer’s disease ,Clinical Sciences ,Neurology & Neurosurgery ,Biochemistry and cell biology - Abstract
BackgroundPharmacologic inhibition of C5aR1, a receptor for the complement activation proinflammatory fragment, C5a, suppressed pathology and cognitive deficits in Alzheimer's disease (AD) mouse models. To validate that the effect of the antagonist was specifically via C5aR1 inhibition, mice lacking C5aR1 were generated and compared in behavior and pathology. In addition, since C5aR1 is primarily expressed on cells of the myeloid lineage, and only to a lesser extent on endothelial cells and neurons in brain, gene expression in microglia isolated from adult brain at multiple ages was compared across all genotypes.MethodsC5aR1 knock out mice were crossed to the Arctic AD mouse model, and characterized for pathology and for behavior performance in a hippocampal dependent memory task. CX3CR1GFP and CCR2RFP reporter mice were bred to C5aR1 sufficient and knockout wild type and Arctic mice to enable sorting of microglia (GFP-positive, RFP-negative) isolated from adult brain at 2, 5, 7 and 10 months of age followed by RNA-seq analysis.ResultsA lack of C5aR1 prevented behavior deficits at 10 months, although amyloid plaque load was not altered. Immunohistochemical analysis showed no CCR2+ monocytes/macrophages near the plaques in the Arctic brain with or without C5aR1. Microglia were sorted from infiltrating monocytes (GFP and RFP-positive) for transcriptome analysis. RNA-seq analysis identified inflammation related genes as differentially expressed, with increased expression in the Arctic mice relative to wild type and decreased expression in the Arctic/C5aR1KO relative to Arctic. In addition, phagosomal-lysosomal gene expression was increased in the Arctic mice relative to wild type but further increased in the Arctic/C5aR1KO mice. A decrease in neuronal complexity was seen in hippocampus of 10 month old Arctic mice at the time that correlates with the behavior deficit, both of which were rescued in the Arctic/C5aR1KO.ConclusionsThese data are consistent with microglial polarization in the absence of C5aR1 signaling reflecting decreased induction of inflammatory genes and enhancement of degradation/clearance pathways, which is accompanied by preservation of CA1 neuronal complexity and hippocampal dependent cognitive function. These results provide links between microglial responses and loss of cognitive performance and, combined with the previous pharmacological approach to inhibit C5aR1 signaling, support the potential of this receptor as a novel therapeutic target for AD in humans.
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- 2017
34. Pre-exposure to Candida albicans induce trans-generational immune priming and gene expression of Musca domestica.
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Zhongxun Li, Lina Jia, Hong Yi, Guo Guo, Li Huang, Yingchun Zhang, Zhenlong Jiao, and Jianwei Wu
- Subjects
PHAGOCYTOSIS ,CANDIDA albicans ,GENE expression ,PATTERN perception receptors ,HOUSEFLY ,RNA sequencing ,CELLULAR signal transduction - Abstract
Insects have the phenomenon of immune priming by which they can have enhanced protection against reinfection with the same pathogen, and this immune protection can be passed on to their offspring, which is defined as "trans-generational immune priming (TGIP)." But whether housefly possesses TGIP is still unclear. Therefore, we used the housefly as the insect model and Candida albicans as the pathogen to explore whether the housefly is capable of eliciting TGIP, and RNA sequencing (RNA-seq) was performed to explore the molecular mechanism of TGIP of the housefly. We found that the housefly possesses TGIP, and adults pre-exposed to heat-killed C. albicans could confer protection to itself and its offspring upon reinfection with a lethal dose of C. albicans. RNA-seq results showed that 30 and 154 genes were differentially expressed after adults were primed with heat-killed C. albicans (CA-A) and after offspring larvae were challenged with a lethal dose of C. albicans (CA-CA-G), respectively. Among the differentially expressed genes (DEGs), there were 23 immune genes, including 6 pattern recognition receptors (PRRs), 7 immune effectors, and 10 immunoregulatory molecules. More importantly, multiple DEGs were involved in the Toll signaling pathway and phagosome signaling pathway, suggesting that the Toll signaling pathway and phagocytosis might play important roles in the process of TGIP of housefly to C. albicans. Our results expanded on previous studies and provided parameters for exploring the mechanism of TGIP. [ABSTRACT FROM AUTHOR]
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- 2022
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35. Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way.
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Santiago-Burgos, Emmanuel J., Stuckey, Peter V., and Santiago-Tirado, Felipe H.
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CRYPTOCOCCUS neoformans ,CANDIDA albicans ,INTRACELLULAR pathogens ,DISEASE management ,PHAGOCYTOSIS ,IMMUNITY ,PHAGOSOMES - Abstract
Understanding of how intracellular pathogens survive in their host cells is important to improve management of their diseases. This has been fruitful for intracellular bacteria, but it is an understudied area in fungal pathogens. Here we start elucidating and characterizing the strategies used by one of the commonest fungal pathogens, Cryptococcus neoformans, to survive intracellularly. The ability of the fungus to survive inside host cells is one of the main drivers of disease progression, yet it is unclear whether C. neoformans resides in a fully acidified, partially acidic, or neutral phagosome. Using a dye that only fluoresce under acidic conditions to stain C. neoformans, a hyphadefective Candida albicans mutant, and the nonpathogenic Saccharomyces cerevisiae, we characterized the fungal behaviors in infected macrophages by live microscopy. The main behavior in the C. albicans mutant strain and S. cerevisiae-phagosomes was rapid acidification after internalization, which remained for the duration of the imaging. In contrast, a significant number of C. neoformans-phagosomes exhibited alternative behaviors distinct from the normal phagosomal maturation: some phagosomes acidified with subsequent loss of acidification, and other phagosomes never acidified. Moreover, the frequency of these behaviors was affected by the immune status of the host cell. We applied the same technique to a flow cytometry analysis and found that a substantial percentage of C. neoformans-phagosomes showed impaired acidification, whereas almost 100% of the S. cerevisiae-phagosomes acidify. Lastly, using a membrane-damage reporter, we show phagosome permeabilization correlates with acidification alterations, but it is not the only strategy that C. neoformans uses to manipulate phagosomal acidification. The different behaviors described here provide an explanation to the confounding literature regarding cryptococcal-phagosome acidification and the methods can be applied to study other intracellular fungal pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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36. ارتباط اینترلوکین 17 و فاگوزوم با ترمیم بعد از انفارکتوس حاد میوکارد.
- Author
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میرزاعلی مفضل جه, کیمیا رسولی پور, زهرا استخر, حامد میر, and اباذر روستازاده
- Subjects
- *
INTERLEUKINS , *SEQUENCE analysis , *INFLAMMATION , *MYOCARDIAL infarction , *MICROARRAY technology , *GENE expression , *CELLULAR signal transduction , *DATA analysis software - Abstract
Acute myocardial infarction is one of the main causes of mortality in the worldwide. The aim of the present study is to predict the Association of interleukin (IL)-17 and phagosome with acute myocardial infarction. Methods and Materials: Microarray data were extracted from National Center for Biotechnology Information (NCBI) and then analyzed by GEO2R and R softwares. The functional analysis of up/down regulation of genes were performed using DAVID and Enrichr data bases. Results: In this study, expression of 208 genes were lower in patients group compared to the controls (Log2FC<-1). In patients’ group, PAQR8, CCR2, CCR5, and ZNF137P genes significantly had lower expression. Although 528 gens, especially NR4A2, GABARAPL1, THBD, NFIL3, and MAFB significantly had more expression compared to the controls (Log2FC>+1). KEGG analysis on gens that increase expression showed that signaling pathways of IL-17 and phagosome are two pathways in patients with acute myocardial infarction. Conclusion: According to our finding, after acute myocardial infarction, inflammatory pathway like IL-17 signaling recruiting matrix metalloproteinase 9 as a protein involve in repairing acute myocardial infarction damages. Also, the phagosome activity by major histocompatibility complex class-II (MHC-II) and CD36 signaling pathways, may be played a role in accelerating healing after acute myocardial infarction damages. [ABSTRACT FROM AUTHOR]
- Published
- 2022
37. Assessment of Non-canonical Functions of the Autophagy Proteins in LC3-Associated Phagocytosis and LC3-Associated Endocytosis.
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Mari L, Boada-Romero E, Li Z, Magné J, and Green DR
- Abstract
The identification and characterization of noncanonical functions within the autophagy pathway have unveiled intricate cellular processes, including LC3-associated phagocytosis (LAP) and LC3-associated endocytosis (LANDO). These phenomena play pivotal roles in the conjugation of ATG8 with single-membrane phagosomes and endosomes, shedding light on the dynamic interplay between autophagy and cellular homeostasis. Here, we present detailed protocols for both qualitative and quantitative assessment of LAP, including immunofluorescence, flow cytometry, and Western blotting of isolated LAPosomes. Additionally, the protocol for the evaluation of LANDO through immunofluorescent detection of receptor recycling is outlined. The methodologies presented herein serve as a practical guide for researchers seeking to unravel the intricacies of LAP and LANDO. By providing step-by-step instructions, accompanied by insights into potential challenges and optimization strategies, this chapter aims to empower investigators in the exploration of these noncanonical functions of autophagy proteins., (© 2024. Springer Science+Business Media, LLC.)
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- 2024
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38. The Role of Membrane Surface Charge in Phagocytosis
- Author
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Maxson, Michelle E., Grinstein, Sergio, Crusio, Wim E., Series Editor, Lambris, John D., Series Editor, Radeke, Heinfried H., Series Editor, Rezaei, Nima, Series Editor, and Hallett, Maurice B., editor
- Published
- 2020
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39. Iris Electron Microscopy
- Author
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Moazed, Kambiz Thomas and Moazed, Kambiz Thomas
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- 2020
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40. Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way
- Author
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Emmanuel J. Santiago-Burgos, Peter V. Stuckey, and Felipe H. Santiago-Tirado
- Subjects
Cryptococcus ,intracellular pathogen ,fungal pathogen ,phagosome ,pHrodo ,galectin-3 ,Microbiology ,QR1-502 - Abstract
Understanding of how intracellular pathogens survive in their host cells is important to improve management of their diseases. This has been fruitful for intracellular bacteria, but it is an understudied area in fungal pathogens. Here we start elucidating and characterizing the strategies used by one of the commonest fungal pathogens, Cryptococcus neoformans, to survive intracellularly. The ability of the fungus to survive inside host cells is one of the main drivers of disease progression, yet it is unclear whether C. neoformans resides in a fully acidified, partially acidic, or neutral phagosome. Using a dye that only fluoresce under acidic conditions to stain C. neoformans, a hypha-defective Candida albicans mutant, and the nonpathogenic Saccharomyces cerevisiae, we characterized the fungal behaviors in infected macrophages by live microscopy. The main behavior in the C. albicans mutant strain and S. cerevisiae-phagosomes was rapid acidification after internalization, which remained for the duration of the imaging. In contrast, a significant number of C. neoformans-phagosomes exhibited alternative behaviors distinct from the normal phagosomal maturation: some phagosomes acidified with subsequent loss of acidification, and other phagosomes never acidified. Moreover, the frequency of these behaviors was affected by the immune status of the host cell. We applied the same technique to a flow cytometry analysis and found that a substantial percentage of C. neoformans-phagosomes showed impaired acidification, whereas almost 100% of the S. cerevisiae-phagosomes acidify. Lastly, using a membrane-damage reporter, we show phagosome permeabilization correlates with acidification alterations, but it is not the only strategy that C. neoformans uses to manipulate phagosomal acidification. The different behaviors described here provide an explanation to the confounding literature regarding cryptococcal-phagosome acidification and the methods can be applied to study other intracellular fungal pathogens.
- Published
- 2022
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41. trim-21 promotes proteasomal degradation of CED-1 for apoptotic cell clearance in C. elegans
- Author
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Lei Yuan, Peiyao Li, Huiru Jing, Qian Zheng, and Hui Xiao
- Subjects
apoptosis ,apoptotic cell clearance ,phagocytic receptor ,E3 ubiquitin ligase ,phagosome ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
The phagocytic receptor CED-1 mediates apoptotic cell recognition by phagocytic cells, enabling cell corpse clearance in Caenorhabditis elegans. Whether appropriate levels of CED-1 are maintained for executing the engulfment function remains unknown. Here, we identified the C. elegans E3 ubiquitin ligase tripartite motif containing-21 (TRIM-21) as a component of the CED-1 pathway for apoptotic cell clearance. When the NPXY motif of CED-1 was bound to the adaptor protein CED-6 or the YXXL motif of CED-1 was phosphorylated by tyrosine kinase SRC-1 and subsequently bound to the adaptor protein NCK-1 containing the SH2 domain, TRIM-21 functioned in conjunction with UBC-21 to catalyze K48-linked poly-ubiquitination on CED-1, targeting it for proteasomal degradation. In the absence of TRIM-21, CED-1 accumulated post-translationally and drove cell corpse degradation defects, as evidenced by direct binding to VHA-10. These findings reveal a unique mechanism for the maintenance of appropriate levels of CED-1 to regulate apoptotic cell clearance.
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- 2022
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- View/download PDF
42. Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome.
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Hong Dong, Bao Zhao, Jianwen Chen, Zihao Liu, Xinghui Li, Lupeng Li, and Haitao Wen
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- *
NLRP3 protein , *INFLAMMASOMES , *KREBS cycle , *MITOCHONDRIA , *ADENOSINE triphosphate , *INFORMATION literacy - Abstract
Mitochondria, a highly metabolically active organelle, have been shown to play an essential role in regulating innate immune function. Mitochondrial Ca2+ uptake via the mitochondrial Ca2+ uniporter (MCU) is an essential process regulating mitochondrial metabolism by targeting key enzymes involved in the tricarboxylic acid cycle (TCA). Accumulative evidence suggests MCU-dependent mitochondrial Ca2+ signaling may bridge the metabolic reprogramming and regulation of immune cell function. However, the mechanism by which MCU regulates inflammation and its related disease remains elusive. Here we report a critical role of MCU in promoting phagocytosis-dependent activation of NLRP3 (nucleotide-binding domain, leucine-rich repeat containing family, pyrin domain-containing 3) inflammasome by inhibiting phagolysosomal membrane repair. Myeloid deletion of MCU (McuΔmye) resulted in an attenuated phagolysosomal rupture, leading to decreased caspase-1 cleavage and interleukin (IL)- 1β release, in response to silica or alum challenge. In contrast, other inflammasome agonists such as adenosine triphosphate (ATP), nigericin, poly(dA:dT), and flagellin induced normal IL-1β release in McuΔmye macrophages. Mechanistically, we demonstrated that decreased NLRP3 inflammasome activation in McuΔmye macrophages was caused by improved phagolysosomal membrane repair mediated by ESCRT (endosomal sorting complex required for transport)-III complex. Furthermore, McuΔmye mice showed a pronounced decrease in immune cell recruitment and IL-1β production in alum-induced peritonitis, a typical IL-1-dependent inflammation model. In sum, our results identify a function of MCU in promoting phagocytosis-dependent NLRP3 inflammatory response via an ESCRT-mediated phagolysosomal membrane repair mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
43. MicroRNAs as Regulators of Phagocytosis.
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Gierlikowski, Wojciech and Gierlikowska, Barbara
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- *
MICRORNA , *NON-coding RNA , *REACTIVE oxygen species , *CELL physiology - Abstract
MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression and thus act as important regulators of cellular phenotype and function. As their expression may be dysregulated in numerous diseases, they are of interest as biomarkers. What is more, attempts of modulation of some microRNAs for therapeutic reasons have been undertaken. In this review, we discuss the current knowledge regarding the influence of microRNAs on phagocytosis, which may be exerted on different levels, such as through macrophages polarization, phagosome maturation, reactive oxygen species production and cytokines synthesis. This phenomenon plays an important role in numerous pathological conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
44. Eructophagy: macrophages use autophagic machinery to burp out parts of their meal.
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Nguyen, Jenny A., Greene, Catherine J., and Yates, Robin M.
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MACROPHAGES ,LYSOSOMES ,MACHINERY ,AUTOPHAGY ,MEALS ,INFLAMMATION - Abstract
The phagolysosome is an antimicrobial and degradative organelle that plays key roles in macrophage-mediated inflammatory and homeostatic functions. Whereas mature phagolysosomes are known to sequester and degrade their contents into basic nutrients, they were not previously assigned an active role in amplifying inflammation. We have described a novel macrophage process in which partially digested immunostimulatory PAMPs are released extracellularly from the mature phagolysosome via discrete events we term eructophagy. Eructophagy is induced by proinflammatory stimuli, negatively regulated by IL4 and MTOR, and is dependent on key autophagy proteins, including fusion machinery of degradative and secretory autophagy. We propose that macrophages use eructophagy to release processed PAMPs/DAMPs to amplify local inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
45. Symbiont-Induced Phagosome Changes Rather than Extracellular Discrimination Contribute to the Formation of Social Amoeba Farming Symbiosis
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Yuehui Tian, Tao Peng, Zhenzhen He, Luting Wang, Xurui Zhang, Zhili He, and Longfei Shu
- Subjects
symbiosis ,chemotaxis ,phagosome ,discrimination ,Dictyostelium discoideum ,Burkholderia ,Microbiology ,QR1-502 - Abstract
ABSTRACT Symbiont recognition is essential in many symbiotic relationships, especially for horizontally transferred symbionts. Therefore, how to find the right partner is a crucial challenge in these symbiotic relationships. Previous studies have demonstrated that both animals and plants have evolved various mechanisms to recognize their symbionts. However, studies about the mechanistic basis of establishing protist-bacterium symbioses are scarce. This study investigated this question using a social amoeba Dictyostelium discoideum and their Burkholderia symbionts. We found no evidence that D. discoideum hosts could distinguish different Burkholderia extracellularly in chemotaxis assays. Instead, symbiont-induced phagosome biogenesis contributed to the formation of social amoeba symbiosis, and D. discoideum hosts have a higher phagosome pH when carrying symbiotic Burkholderia than nonsymbiotic Burkholderia. In conclusion, the establishment of social amoeba symbiosis is not linked with extracellular discrimination but related to symbiont-induced phagosome biogenesis, which provides new insights into the mechanisms of endosymbiosis formation between protists and their symbionts. IMPORTANCE Protists are single-celled, extremely diverse eukaryotic microbes. Like animals and plants, they live with bacterial symbionts and have complex relationships. In protist-bacterium symbiosis, while some symbionts are strictly vertically transmitted, others need to reestablish and acquire symbionts from the environment frequently. However, the mechanistic basis of establishing protist-bacterium symbioses is mostly unclear. This study uses a novel amoeba-symbiont system to show that the establishment of this symbiosis is not linked with extracellular discrimination. Instead, symbiont-induced phagosome biogenesis contributes to the formation of social amoeba-bacterium symbiosis. This study increases our understanding of the mechanistic basis of establishing protist-bacterium symbioses.
- Published
- 2022
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46. Staphylococcus aureus promotes its intracellular survival by inhibiting Rab11-Rab11FIP4-mediated vesicle trafficking.
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Xu, Huiling, Wang, Xiaozhou, Zhang, Zhizhong, Hu, Jiaqing, Yu, Yongtao, Wang, Jiandong, Liu, Yongxia, and Liu, Jianzhu
- Subjects
- *
BOVINE mastitis , *MAMMARY glands , *EPITHELIAL cells , *DAIRY cattle , *PHAGOSOMES , *LYSOSOMES - Abstract
Mastitis in dairy cows is mainly caused by bacteria, in which Staphylococcus aureus appears frequently. Epithelial cells, as a major physical barrier of mammary gland, play an important role in preventing mastitis in dairy cows. Our previous study reported that Rab11fip4 (an effector of Rab11) was significantly changed in response to stimulation by S. aureus. So, in this study, the role of Rab11A in phagocytosis of bovine mammary epithelial cells (MAC-T) against S. aureus was evaluated. First, changes of Rab11A and Rab11fip4 were analyzed in response to S. aureus by immunofluorescence and western blotting. Subsequently, the effects of Rab11A and Rab11fip4 on proliferation of S. aureus , as well as formation and function of late endosomes (LEs) and lysosomes (LYSs) were investigated. The results showed that, after infection, Rab11A and Rab11fip4 were recruited to phagosomes containing S. aureus. Rab11A promoted bacterial clearance and rescues the destruction of LEs and LYSs by S. aureus , whereas Rab11fip4 did the opposite. These findings provide new insights into phagocytosis and control of S. aureus in host cells, thus lay the foundation to elucidate the pathogenesis of S. aureus in bovine mastitis. • Rab11A and Rab11fip4 were recruited to the phagosomes containing S. aureus. • Rab11A restored the function of late endosomes and lysosomes stimulated by S.aureus and promoted the clearance of S. aureus. • Rab11fip4 blocked phagosomal biogenesis and assisted S. aureus to survive in bovine mammary epithelial cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. Proteomics analysis of differentially abundant proteins in the rohu kidney infected with Edwardsiella tarda.
- Author
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Pinto, Nevil, Nissa, Mehar Un, Yashwanth, B.S., Sathiyanarayanan, A., Pai, Medha Gayathri J., Srivastava, Sanjeeva, and Goswami, Mukunda
- Subjects
EDWARDSIELLA tarda ,ROHU ,PROTEOMICS ,METABOLIC reprogramming ,KIDNEYS ,GRAM-negative bacteria ,PROTEIN-protein interactions - Abstract
Edwardsiella tarda (Et) is a zoonotic gram-negative pathogen with a diverse host range, including fish. However, the in-depth molecular mechanisms underlying the response of Labeo rohita (rohu) kidney to Et are poorly understood. A proteomic and histopathological analysis was performed for the rohu kidney after Et infection. The histopathology of the infected rohu kidney showed vacuolation and necrosis. After LC-MS/MS analysis, ~1240 proteins were identified with ≥2 unique peptides. A total of 96 differentially abundant proteins (DAPs) were observed between the control and Et infected group (ET). Metascape and STRING analysis were used for the gene ontology (GO), and protein-protein interaction network (PPI) for the significant pathways of DAPs. In PPI, low-abundant proteins were mapped to metabolic pathways and oxidative phosphorylation (cox5ab, uqcrfs1). High-abundance proteins were mapped to ribosomes (rplp2), protein process in the ER (hspa8), and immune system (ptgdsb.1, muc2). Our label-free proteomic approach in the rohu kidney revealed abundant enriched proteins involved in vesicle coat (ehd4), complement activation (c3a.1, c9, c7a), phagosome (thbs4, mapk1), metabolic reprogramming (hao1, glud1a), wound healing (vim, alox5), and the immune system (psap) after Et infection. A targeted proteomics approach of multiple reaction monitoring (MRM) validated the DAPs (nprl3, ambp, vmo1a, hspg2, muc2, hao1 and glud1a) between control and ET. Overall, the current analysis of histology and proteome in the rohu kidney provides comprehensive data on pathogenicity and the potential immune proteins against Et. [Display omitted] • Edwardsiella tarda (Et) affects rohu and disease mechanism is poorly understood. • Label-free quantification (LFQ) approach was performed for Et infected rohu kidney. • Quantitative proteomic profiling of rohu against Et showed 96 differentially abundant proteins. • Et impaired kidney proteins involved in renal metabolic, wound healing and immune mechanism. • The altered proteins validated using MRM and may serve as valuable markers of infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
48. Cathepsin S are involved in human carotid atherosclerotic disease progression, mainly by mediating phagosomes: bioinformatics and in vivo and vitro experiments.
- Author
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Hailong Wang, Haiying Jiang, and Xian Wu Cheng
- Subjects
PHAGOSOMES ,ATHEROSCLEROTIC plaque ,FOAM cells ,DISEASE progression ,GENE expression - Abstract
Background: Atherosclerosis emerges as a result of multiple dynamic cell processes including endothelial damage, inflammatory and immune cell infiltration, foam cell formation, plaque rupture, and thrombosis. Animal experiments have indicated that cathepsins (CTSs) mediate the antigen transmission and inflammatory response involved in the atherosclerosis process, but the specific signal pathways and target cells of the CTSs involved in atherosclerosis are unknown. Methods: We used the GEO query package to download the dataset GSE28829 from the Gene Expression Omnibus (GEO) and filtered the data to check the standardization of the samples through the box chart. We then used the 'limma' package to analyze between-group differences and selected the corresponding differentially expressed genes of CTSs from the protein-protein interaction (PPI) network constructed with the STRING database, and then visualized the CTS-target genes. The best matching pathway and target cells were verified by a male mouse ligation experiment, single-sample GSEA (ssGSEA) analysis, and vitro experiment. Results: There were 275 differentially expressed genes (DEGs) selected from the GSE28829 dataset, and the DEGs were identified mainly in the PPI network; 58 core genes (APOE, CD74, CP, AIF1, etc.) target three selected CTS family members (CTSS, CTSB, and CTSC). After the enriched analysis, 15 CTS-target genes were markedly enriched in the phagosome signaling pathway. The mouse experiment results revealed that the percentages and numbers of monocytes and neutrophils and the number of CD68
+ cells in CTSS deficiency (CatS-/- ) group were lower than those in the wildtype (CatS+/+ ) group. CTSS mediating phagosome via macrophage were further verified by ssGSEA analysis and vitro experiment. Conclusions: CTSS are the main target molecules in the CTS family that are involved in atherosclerosis. The molecule participate in the progression of atherosclerosis by mediating the phagosome via macrophage. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
49. Mycobacterial response to an acidic environment: protective mechanisms.
- Author
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Rai, Rupal, Singh, Vinayak, Mathew, Bijina J, Singh, Anirudh K, and Chaurasiya, Shivendra K
- Subjects
- *
MYCOBACTERIUM tuberculosis , *HELICOBACTER pylori , *LISTERIA monocytogenes , *GLUTAMATE decarboxylase , *PHAGOSOMES , *ESCHERICHIA coli - Abstract
Given the emergence and spread of multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis (Mtb), the world faces the urgency of finding new drugs to combat tuberculosis. Understanding the biochemical/physiological processes enabling Mtb to survive the stressful environment within macrophages and acquire tolerance, resistance and persistence against the stresses are the key to developing new approaches to tackle this health problem. As Mtb gains entry into the respiratory tract and is engulfed by macrophages, lowering pH acts as a primary defence of phagosomes within macrophages and also in the centres of caseating granulomas. It becomes essential for the pathogen to maintain pH homeostasis for survival in these conditions. Acid resistance mechanisms are well known and extensively studied in other bacteria such as Escherichia coli , Lactobacillus spp. Brucella spp. Helicobacter pylori and Listeria monocytogenes. However, in the case of Mtb, acid tolerance and resistance mechanisms still need to be explored in detail. This review aims to describe the current understanding of underlying mechanisms involved in countering low pH faced by Mtb as the acid resistance/tolerance mechanisms contribute to the pathogenesis of the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
50. Leishmania donovani infection induce Extracellular signal-regulated kinase ½ (ERK½) mediated lipid droplet generation in macrophages.
- Author
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Banerjee, Somenath, Bose, Dipayan, Das, Subhadip, Chatterjee, Nabanita, Mishra, Snehasish, and Das Saha, Krishna
- Subjects
- *
LEISHMANIA donovani , *EXTRACELLULAR signal-regulated kinases , *MACROPHAGES , *LIPIDS , *CONFOCAL microscopy , *FLOW cytometry , *LYSOSOMES , *PERITONEAL macrophages - Abstract
[Display omitted] • L. donovani ag83 induce LD generation in macrophages. • ERK1/2 is responsible for LD generation. • Neutralization of LD generation restores phago-lysosome biogenesis. • Diminishing LD generation also correlated with augmentation of IL-12. Recently unfolded mechanisms showed lipid droplet helps in pathogen survival and paralyzes host immune response. In the present study, we showed the extent of lipid droplet(LD) generation in Leishmania donovani infection, the signaling involved, and their function concerning pathogenicity. RAW 264.7 and J774A.1 cells were used to infect with L. donovani and then flow cytometry and confocal microscopy were used to detect lipid droplet generation and subsequent assays. In this study, we showed that L. donovani AG83 (AG83/MHOM/1983) triggers lipid droplet formation in macrophages in a time-dependent manner. We provide novel insight into the signaling molecules which is responsible for LD accumulation. Interestingly, LPG deficient attenuated Leishmania strain UR6 (UR6/MHOM/1978) failed to fuel LD generation. But inhibition of phagosome maturation drastically stimulates LD accumulation in UR6 infected MΦs. Aspirin treatment in AG83 infected MΦs does not only lower LD load but also favors phagolysosome biogenesis and corrects cytokine balance. Employing strategies to circumvent halt in phagosome maturation using drugs that manipulate lipid droplet generation could be used as a therapeutic tool to resist parasite growth in the early hour of infection. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
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