176 results on '"Phillips WD"'
Search Results
2. Fire Resistance Tests for Fluids and Lubricants — Their Limitations and Misapplication
- Author
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Phillips, WD, primary
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- 1996
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3. Proliferation, differentiation and migration of SCA1−/CD31− cardiac side population cells in vitro and in vivo
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Wang, XZ, Gao, RL, Sun, P, Liu, S, Xu, Y, Liang, DZG, Yin, LM, Phillips, WD, and Liang, SX
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Myocardial Infarction ,Cell Culture Techniques ,Endothelial Cells ,Cell Differentiation ,Antigens, CD31 ,Mice, Inbred C57BL ,Platelet Endothelial Cell Adhesion Molecule-1 ,Mice ,Disease Models, Animal ,Cardiovascular System & Hematology ,Cell Movement ,cardiovascular system ,Animals ,Myocytes, Cardiac ,Female ,Side-Population Cells ,Ataxin-1 ,Cell Proliferation - Abstract
© 2016 Elsevier Ireland Ltd Background Side-population (SP) cells, identified by their capacity to efflux Hoechst dye, are highly enriched for stem/progenitor cell activity. They are found in many mammalian tissues, including mouse heart. Studies suggest that cardiac SP (CSP) cells can be divided into SCA1+/CD31−, SCA1+/CD31+and SCA1−/CD31−CSP subpopulations. SCA1+/CD31−were shown to be cardiac and endothelial stem/progenitors while SCA1+/CD31+CSP cells are endothelial progenitors. SCA1−/CD31−CSP cells remain to be fully characterized. In this study, we characterized SCA1−/CD31−CSP cells in the adult mouse heart, and investigated their abilities to proliferate, differentiate and migrate in vitro and in vivo. Methods and results Using fluorescence-activated cell sorting, reverse transcriptase/polymerase chain reaction, assays of cell proliferation, differentiation and migration, and a murine model of myocardial infarction we show that SCA1−/CD31−CSP cells are located in the heart mesenchyme and express genes characteristic of stem cells and endothelial progenitors. These cells were capable of proliferation, differentiation, migration and vascularization in vitro and in vivo. Following experimental myocardial infarction, the SCA1−/CD31−CSP cells migrated from non-infarcted areas to the infarcted region within the myocardium where they differentiated into endothelial cells forming vascular (tube-like) structures. We further demonstrated that the SDF-1α/CXCR4 pathway may play an important role in migration of these cells after myocardial infarction. Conclusions Based on their gene expression profile, localization and ability to proliferate, differentiate, migrate and vascularize in vitro and in vivo, we conclude that SCA1−/CD31−CSP cells may serve as endothelial progenitor cells in the adult mouse heart.
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- 2016
4. Chapter 12—Turbine Lubricating Oils and Hydraulic Fluids
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Totten, GE, primary, Westbrook, SR, additional, Shah, RJ, additional, and Phillips, WD, additional
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5. Sensitivity to the frequency of parts and kinds: two principles of organisation in semantic memory
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Jenei J, Chaffin R, and Phillips Wd
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Communication ,business.industry ,Superordinate goals ,Task (project management) ,Semantics ,Arts and Humanities (miscellaneous) ,Memory ,Semantic memory ,Humans ,Sensitivity (control systems) ,business ,Psychology ,Association (psychology) ,General Psychology ,Cognitive psychology - Abstract
Are subjects sensitive to the frequency of parts of the same whole in the same way that they are sensitive to instances of the same category? Subjects read a randomised list of words and then estimated how many words on the list had belonged to particular groups, e.g. parts of a bird, or kinds of bird. Groups of words were either parts of the same whole, e.g. feathers, beak, wing, or members of the same category, e.g. robin, sparrow, eagle. Subjects were asked, either, "How many parts of a (bird) did you see?" or "How many kinds of (bird) did you see?". Half of the subjects expected the frequency estimation task, whereas for the other half, who performed a distractor task during encoding, the task was unexpected. In Experiment 1 subjects saw either parts of kinds. Sensitivity to frequency did not differ for parts and kinds nor as a function of whether the frequency estimation test was expected. In Experiment 2 subjects saw both parts and kinds belonging to the same superordinates, e.g. parts of birds and kinds of birds, and were asked to estimate the frequency of both types of groupings for each superordinate. The results were the same as in Experiment 1. In addition, subjects were able to separate the frequency of parts and kinds of the same superordinate. The results suggest that, in frequency estimation, part-of and kind-of associations can function similarly, and that the mechanism responsible for frequency estimation is sensitive to the type of association.
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- 1996
6. A Discrepancy Between two Modes of Haptic Length Perception
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Phillips Wd, VanRomer H, and Hohmuth A
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Male ,medicine.medical_specialty ,Visual perception ,genetic structures ,media_common.quotation_subject ,Illusion ,Audiology ,Thumb ,behavioral disciplines and activities ,Education ,Fingers ,Orientation ,Perception ,medicine ,Humans ,Size Perception ,General Psychology ,media_common ,Haptic technology ,Visual comparison ,Index finger ,Illusions ,body regions ,medicine.anatomical_structure ,Touch ,Visual Perception ,Business, Management and Accounting (miscellaneous) ,Female ,Psychology ,Social psychology ,psychological phenomena and processes - Abstract
This study investigated the possibility that systematic differences exist between the perceptions resulting from the use of two different heptic modes to know the same object. In each of three experiments 24 male and female college students chose visual comparison stimuli to match the length of haptically presented standard stimuli. In each experiment significantly smaller visual matches were made when the length of the haptic standard was perceived by holding it between the thumb and index finger than when the haptic standard was perceived by stroking it from end to end with the index finger. Experiment 1 established a significant difference (p less than .05) using a horizontal visual comparison. Experiment 2 replicated this effect (p less than .001) using vertical visual comparisons. Experiment 3 again replicated the effect (p less than .001) with visual comparisons and ruled out the possibility that the effect was due to the orientation of the haptic stimuli.
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- 1976
7. Proton magnetic resonance studies of Desulfovibrio cytochromes c3
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Phillips Wd, LeGall J, and McDonald Cc
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Magnetic Resonance Spectroscopy ,Chemical Phenomena ,biology ,Chemistry ,Iron ,Temperature ,Cytochrome c Group ,Heme ,Deuterium ,biology.organism_classification ,Biochemistry ,Desulfovibrio ,Proton magnetic resonance ,Magnetics ,Nuclear magnetic resonance ,Species Specificity ,Histidine ,Protons ,Oxidation-Reduction - Published
- 1974
8. Proton magnetic resonance study of the indole NH resonances of lysozyme. Assignment, deuterium exchange kinetics, and inhibitor binding
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Phillips Wd, Glickson Jd, and Rupley Ja
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Protein Denaturation ,Indoles ,Magnetic Resonance Spectroscopy ,Ovalbumin ,Protein Conformation ,Kinetics ,Photochemistry ,Biochemistry ,Catalysis ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,X-Ray Diffraction ,Amino Acid Sequence ,Indole test ,Tryptophan ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Deuterium ,Crystallography ,chemistry ,Spectrophotometry ,X-ray crystallography ,Hydrogen–deuterium exchange ,Muramidase ,Lysozyme ,Protons ,Protein Binding - Published
- 1971
9. Role of rapsyn in agrin-mediated acetylcholine receptor clustering
- Author
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Han, H., Peter Noakes, and Phillips, Wd
10. Quality Control of Motor Unit Number Index (MUNIX) Measurements in 6 Muscles in a Single-Subject 'Round-Robin' Setup
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Markus Weber, Cristina Moglia, Maarten Schrooten, Malgorzata Gawel, James J.P. Alix, José Castro, Timothée Lenglet, Julian Grosskreutz, Taha Omer, Christian Burkhardt, Christoph Neuwirth, Mamede de Carvalho, Stephan Goedee, Phillips, William D, and Phillips, WD
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Genetics and Molecular Biology (all) ,0301 basic medicine ,Male ,Research Validity ,Physiology ,lcsh:Medicine ,Electromyography ,Medicine (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Agricultural and Biological Sciences (all) ,Biceps ,Biochemistry ,Motor Neuron Diseases ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Medicine ,Amyotrophic lateral sclerosis ,lcsh:Science ,Neurons ,Motor Neurons ,Multidisciplinary ,medicine.diagnostic_test ,Healthy subjects ,Neurodegenerative Diseases ,Muscle Analysis ,Neuromuscular Diseases ,Research Assessment ,Healthy Volunteers ,3. Good health ,Electrophysiology ,Bioassays and Physiological Analysis ,Neurology ,Female ,Cellular Types ,Muscle Electrophysiology ,Research Article ,medicine.medical_specialty ,Coefficient of variation ,education ,Neurophysiology ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,Sensitivity and Specificity ,03 medical and health sciences ,Physical medicine and rehabilitation ,Functional electrical stimulation ,Humans ,Muscle Strength ,Muscle, Skeletal ,Functional Electrical Stimulation ,business.industry ,lcsh:R ,Electrophysiological Techniques ,Amyotrophic Lateral Sclerosis ,Motor unit number ,Biology and Life Sciences ,Reproducibility of Results ,Abductor hallucis ,Cell Biology ,medicine.disease ,030104 developmental biology ,Cellular Neuroscience ,Physical therapy ,lcsh:Q ,business ,030217 neurology & neurosurgery ,Biomarkers ,Neuroscience - Abstract
Background\ud Motor Unit Number Index (MUNIX) is a neurophysiological measure that provides an index\ud of the number of lower motor neurons in a muscle. Its performance across centres in healthy\ud subjects and patients with Amyotrophic Lateral Sclerosis (ALS) has been established, but\ud inter-rater variability between multiple raters in one single subject has not been\ud investigated.\ud Objective\ud To assess reliability in a set of 6 muscles in a single subject among 12 examiners (6 experienced\ud with MUNIX, 6 less experienced) and to determine variables associated with variability\ud of measurements.\ud Methods\ud Twelve raters applied MUNIX in six different muscles (abductor pollicis brevis (APB),\ud abductor digiti minimi (ADM), biceps brachii (BB), tibialis anterior (TA), extensor dig. brevis\ud (EDB), abductor hallucis (AH)) twice in one single volunteer on consecutive days. All raters\ud visited at least one training course prior to measurements. Intra- and inter-rater variability as\ud determined by the coefficient of variation (COV) between different raters and their levels of\ud experience with MUNIX were compared.\ud Results\ud Mean intra-rater COV of MUNIX was 14.0% (±6.4) ranging from 5.8 (APB) to 30.3% (EDB).\ud Mean inter-rater COV was 18.1 (±5.4) ranging from 8.0 (BB) to 31.7 (AH). No significant differences\ud of variability between experienced and less experienced raters were detected.\ud Conclusion\ud We provide evidence that quality control for neurophysiological methods can be performed\ud with similar standards as in laboratory medicine. Intra- and inter-rater variability of MUNIX is\ud muscle-dependent and mainly below 20%. Experienced neurophysiologists can easily\ud adopt MUNIX and adequate teaching ensures reliable utilization of this method.
- Published
- 2016
11. Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease.
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Ding Q, Kesavan K, Lee KM, Wimberger E, Robertson T, Gill M, Power D, Chang J, Fard AT, Mar JC, Henderson RD, Heggie S, McCombe PA, Jeffree RL, Colditz MJ, Hilliard MA, Ng DCH, Steyn FJ, Phillips WD, Wolvetang EJ, Ngo ST, and Noakes PG
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- Humans, LDL-Receptor Related Proteins metabolism, Receptors, Cholinergic metabolism, Signal Transduction, Agrin metabolism, Motor Neuron Disease
- Abstract
A central event in the pathogenesis of motor neuron disease (MND) is the loss of neuromuscular junctions (NMJs), yet the mechanisms that lead to this event in MND remain to be fully elucidated. Maintenance of the NMJ relies upon neural agrin (n-agrin) which, when released from the nerve terminal, activates the postsynaptic Muscle Specific Kinase (MuSK) signaling complex to stabilize clusters of acetylcholine receptors. Here, we report that muscle from MND patients has an increased proportion of slow fibers and muscle fibers with smaller diameter. Muscle cells cultured from MND biopsies failed to form large clusters of acetylcholine receptors in response to either non-MND human motor axons or n-agrin. Furthermore, levels of expression of MuSK, and MuSK-complex components: LRP4, Caveolin-3, and Dok7 differed between muscle cells cultured from MND patients compared to those from non-MND controls. To our knowledge, this is the first time a fault in the n-agrin-LRP4-MuSK signaling pathway has been identified in muscle from MND patients. Our results highlight the n-agrin-LRP4-MuSK signaling pathway as a potential therapeutic target to prolong muscle function in MND., (© 2022. The Author(s).)
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- 2022
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12. Advances in the understanding of disease mechanisms of autoimmune neuromuscular junction disorders.
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Huijbers MG, Marx A, Plomp JJ, Le Panse R, and Phillips WD
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- Humans, Neuromuscular Junction, Neuromuscular Junction Diseases
- Abstract
Muscle weakness and fatigue are the hallmarks of autoimmune neuromuscular junction disorders. Although a plethora of immunosuppressive treatments exist, no cure is available to date and many patients are left with debilitating muscle weakness. Recent advances in the understanding of the structure and function of the neuromuscular junction, and the development of novel in vitro and in vivo models, have been instrumental in unravelling the pathophysiology of these autoimmune diseases. These advances are providing the rationale for the development of new therapeutic strategies. Restoration of the immune imbalance in these diseases, in parallel with symptomatic therapeutic approaches at the neuromuscular junction, will be crucial to obtain long-term remission or even cure., Competing Interests: Declaration of interests MGH reports financial support paid to her institution from Leiden University Medical Center (OIO, 2017), Top Sector Life Sciences & Health to Samenwerkende Gezondheidsfondsen via the Target to B! consortium (LSHM18055-SGF), Prinses Beatrix Spierfonds (W.OR-17.13 and W.OR-19.13), and the Dutch Science Organization NWO (VENI 0915016181 0040); reports royalties for a diagnostic MuSK ELISA; and is a coinventor on two patent applications on MuSK-related research. AM is on the medical advisory board for the German Myasthenia Gravis Association. JJP reports research support paid to his institution from Association Française contre les Myopathies, Prinses Beatrix Spierfonds, the Dutch Science Organization NWO, and Alnylam Pharmaceuticals; and is a coinventor on two patent applications on MuSK-related research. RLP reports financial support paid to their institution from the Association Française contre les Myopathies. WDP reports financial support from the Lambert Initiative for Cannabinoid Therapeutics and Bridging Grants from the University of Sydney. The funders had no role in the writing of this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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13. Vector-mediated expression of muscle specific kinase restores specific force to muscles in the mdx mouse model of Duchenne muscular dystrophy.
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Ban J, Beqaj B, and Phillips WD
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- Animals, Dystrophin metabolism, Mice, Mice, Inbred mdx, Muscle Contraction physiology, Muscle Strength, Muscle, Skeletal physiology, Muscular Dystrophy, Duchenne metabolism
- Abstract
New Findings: What is the central question of this study? The (dystrophin-deficient) muscles of mdx mice generate less contractile force per cross-sectional area (specific force) than those of healthy wild-type mice: what is the influence of muscle specific kinase (MuSK) upon the properties of the tibialis anterior (TA) muscle in mdx mice? What is the main finding and its importance? Injection of adeno-associated viral vector encoding MuSK into the TA muscle of young mdx mice increased the specific force of the muscle, suggesting the MuSK signalling system has the potential to restore healthy growth to dystrophin-deficient muscles., Abstract: In the mdx mouse model of Duchenne muscular dystrophy, muscle fibres are fragile and prone to injury and degeneration. Compared to wild-type mice, muscles of mdx mice also develop less specific force (contractile force/cross-sectional area). We recently reported that injecting adeno-associated viral vector encoding muscle specific kinase (AAV-MuSK) into muscles of mdx mice increased utrophin expression and made the muscles more resistant to acute stretch-induced injury. Here we injected AAV-MuSK unilaterally into the tibialis anterior muscle of mdx mice at a younger age (4 weeks), and recorded contraction force from the muscles in situ at 12 weeks of age. Compared to contralateral empty-vector control muscles, muscles injected with AAV-MuSK produced 28% greater specific force (P = 0.0005). They did not undergo the compensatory hypertrophy that normally occurs in muscles of mdx mice. Injection of AAV encoding rapsyn (a downstream effector of MuSK signalling) caused no such improvement in muscle strength. Muscles injected with AAV-MuSK displayed a 10% reduction in the number of fibres with centralized nuclei (P = 0.0015). Our results in mdx mice suggest that elevating the expression of MuSK can reduce the incidence of muscle fibre regeneration and improve the strength of dystrophin-deficient muscles., (© 2021 The Authors. Experimental Physiology © 2021 The Physiological Society.)
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- 2021
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14. The Safety Factor for Neuromuscular Transmission: Effects of Dimethylsulphoxide, Cannabinoids and Synaptic Homeostasis.
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Odierna GL and Phillips WD
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- Action Potentials, Animals, Diaphragm physiopathology, Mice, Motor Endplate, Muscle Contraction, Neuromuscular Junction drug effects, Receptors, Cholinergic, Synaptic Transmission drug effects, Tubocurarine pharmacology, Cannabinoids pharmacology, Dimethyl Sulfoxide pharmacology, Homeostasis drug effects, Myasthenia Gravis physiopathology
- Abstract
BackgroundIn myasthenia gravis, impaired postsynaptic sensitivity to acetylcholine results in failure of neuromuscular transmission and fatiguing muscle weakness.ObjectiveDevelop an ex vivo muscle contraction assay to test cannabinoids and other substances that might act on the myasthenic neuromuscular junction to restore control of the muscle.MethodsTubocurarine was added to an ex vivo, mouse phrenic nerve-hemidiaphragm muscle preparation to reduce acetylcholine sensitivity. This produced a myasthenia-like decrement in twitch force during a train of 10 nerve impulses (3 / sec). Endplate potential (EPP) recordings were used to confirm and extend the findings.ResultsSurprisingly, addition to the bath of dimethylsulphoxide (DMSO), at concentrations as low as 0.1%(v/v), partially reversed the decrement in nerve-evoked force. Intracellular electrophysiology, conducted in the presence of tubocurarine, showed that DMSO increased the amplitudes of both the spontaneous miniature EPP (MEPP) and the (nerve-evoked) EPP. In the absence of tubocurarine (synaptic potentials at physiological levels), an adaptive fall in quantal content negated the DMSO-induced rise in EPP amplitude. The effects of cannabinoid receptor agonists (solubilized with DMSO) in the contraction assay do not support their further exploration as useful therapeutic agents for myasthenia gravis. CP 55,940 (a dual agonist for cannabinoid receptor types 1 and 2) reversed the beneficial effects of DMSO.Conclusions:We demonstrate a powerful effect of DMSO upon quantal amplitude that might mislead pharmacological studies of synaptic function wherever DMSO is used as a drug vehicle. Our results also show that compounds targeting impaired neuromuscular transmission should be tested under myasthenic-like conditions, so as to avoid confounding effects of synaptic homeostasis.
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- 2021
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15. Corrigendum: Revision of Fothergilla (Hamamelidaceae), including resurrection of F. parvifolia and a new species, F. milleri .
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Haynes JE, Phillips WD, Krings A, Lynch NP, and Ranney TG
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[This corrects the article DOI: 10.3897/phytokeys.144.49589.]., (Jake E. Haynes, Whitney D. Phillips, Alexander Krings, Nathan P. Lynch, Thomas G. Ranney.)
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- 2020
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16. Influence of cannabinoids upon nerve-evoked skeletal muscle contraction.
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Ge D, Odierna GL, and Phillips WD
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- Animals, Cannabinoids pharmacology, Humans, Motor Neurons drug effects, Muscle Contraction drug effects, Muscle, Skeletal drug effects, Signal Transduction drug effects, Signal Transduction physiology, Synaptic Transmission drug effects, Synaptic Transmission physiology, Cannabinoids metabolism, Motor Neurons metabolism, Muscle Contraction physiology, Muscle, Skeletal innervation, Muscle, Skeletal metabolism
- Abstract
Endocannabinoids play important roles in regulating CNS synaptic function and peripheral metabolism, but cannabinoids can also act acutely to modulate contraction strength in skeletal muscle. Nerve terminals and the skeletal muscle sarcolemma express components of the cannabinoid signaling system. Endocannabinoids, N-arachidonylethanolamine (anandamide, AEA) and 2-arachidonoyl-glycerol (2-AG), are produced by skeletal muscle. They may be involved in the acute regulation of neuromuscular transmission, by adjusting the parameters for quantal acetylcholine release from the motor nerve terminal. Downstream of neuromuscular transmission, cannabinoids may also act to limit the efficiency of excitation-contraction coupling. Improved understanding of the distinct signaling actions of particular cannabinoid compounds and their receptor/transduction systems will help advance our understanding of the role of endocannabinoids in skeletal muscle physiology. Cannabinoids might also offer the potential to develop new pharmacotherapeutics to treat neuromuscular disorders that affect muscle strength., Competing Interests: Declaration of Competing Interest The authors received grant support for their cannabinoid research from the Lambert Initiative for Cannabinoid Therapeutics (to WDP)., (Copyright © 2020 Elsevier B.V. All rights reserved.)
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- 2020
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17. Revision of Fothergilla (Hamamelidaceae), including resurrection of F. parvifolia and a new species, F. milleri .
- Author
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Haynes JE, Phillips WD, Krings A, Lynch NP, and Ranney TG
- Abstract
Fothergilla is a small genus of deciduous shrubs native to the southeastern United States that depending on circumscription comprises two to four species. Recent treatments recognized only two species in the genus: F. gardenii (tetraploid) and F. major (hexaploid). Until recently, no diploid taxon of Fothergilla was known. However, recent investigations identified a number of diploid populations in Alabama, Florida, Georgia, and South Carolina. A subsequent phylogenomic analysis showed that the diploids segregated into two, well-supported lineages, corresponding to largely allopatric populations. A re-examination of the morphology of diploid plants, in combination with the genetic evidence, has led us to the recognition of two species of diploids in the genus - a resurrected F. parvifolia and a new species ( F. milleri W.D. Phillips & J.E. Haynes, sp. nov. ) - bringing the total number of recognized species in Fothergilla to four. A revised taxonomic treatment of the genus is provided., (Jake E. Haynes, Whitney D. Phillips, Alexander Krings, Nathan P. Lynch, Thomas G. Ranney.)
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- 2020
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18. Muscle specific kinase protects dystrophic mdx mouse muscles from eccentric contraction-induced loss of force-producing capacity.
- Author
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Trajanovska S, Ban J, Huang J, Gregorevic P, Morsch M, Allen DG, and Phillips WD
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- Animals, Disease Models, Animal, Dystrophin metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Inbred mdx, Muscle Strength physiology, Neuromuscular Junction metabolism, Sarcolemma metabolism, Signal Transduction physiology, Utrophin metabolism, Muscle Contraction physiology, Muscle, Skeletal metabolism, Muscle, Skeletal physiology, Muscular Dystrophy, Duchenne metabolism, Receptor Protein-Tyrosine Kinases metabolism
- Abstract
Key Points: Adeno-associated viral vector was used to elevate the expression of muscle specific kinase (MuSK) and rapsyn (a cytoplasmic MuSK effector protein) in the tibialis anterior muscle of wild-type and dystrophic (mdx) mice. In mdx mice, enhanced expression of either MuSK or rapsyn ameliorated the acute loss of muscle force associated with strain injury. Increases in sarcolemmal immunolabelling for utrophin and β-dystroglycan suggest a mechanism for the protective effect of MuSK in mdx muscles. MuSK also caused subtle changes to the structure and function of the neuromuscular junction, suggesting novel roles for MuSK in muscle physiology and pathophysiology., Abstract: Muscle specific kinase (MuSK) has a well-defined role in stabilizing the developing mammalian neuromuscular junction, but MuSK might also be protective in some neuromuscular diseases. In the dystrophin-deficient mdx mouse model of Duchenne muscular dystrophy, limb muscles are especially fragile. We injected the tibialis anterior muscle of 8-week-old mdx and wild-type (C57BL10) mice with adeno-associated viral vectors encoding either MuSK or rapsyn (a cytoplasmic MuSK effector protein) fused to green fluorescent protein (MuSK-GFP and rapsyn-GFP, respectively). Contralateral muscles injected with empty vector served as controls. One month later mice were anaesthetized with isoflurane and isometric force-producing capacity was recorded from the distal tendon. MuSK-GFP caused an unexpected decay in nerve-evoked tetanic force, both in wild-type and mdx muscles, without affecting contraction elicited by direct electrical stimulation of the muscle. Muscle fragility was probed by challenging muscles with a strain injury protocol consisting of a series of four strain-producing eccentric contractions in vivo. When applied to muscles of mdx mice, eccentric contraction produced an acute 27% reduction in directly evoked muscle force output, affirming the susceptibility of mdx muscles to strain injury. mdx muscles overexpressing MuSK-GFP or rapsyn-GFP exhibited significantly milder force deficits after the eccentric contraction challenge (15% and 14%, respectively). The protective effect of MuSK-GFP in muscles of mdx mice was associated with increased immunolabelling for utrophin and β-dystroglycan in the sarcolemma. Elevating the expression of MuSK or rapsyn revealed several distinct synaptic and extrasynaptic effects, suggesting novel roles for MuSK signalling in muscle physiology and pathophysiology., (© 2019 The Authors. The Journal of Physiology © 2019 The Physiological Society.)
- Published
- 2019
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19. Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro.
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Xu Y, Sun P, Wang JY, Li ZZ, Gao RL, Wang XZ, Phillips WD, and Liang SX
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- Animals, Biomarkers, Cell Line, Tumor, Cell Separation methods, Cells, Cultured, Colony-Forming Units Assay, Female, Fluorescent Antibody Technique, Gene Expression, Immunophenotyping, Leukocyte Common Antigens genetics, Leukocyte Common Antigens metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Platelet Endothelial Cell Adhesion Molecule-1 genetics, Platelet Endothelial Cell Adhesion Molecule-1 metabolism, Cell Differentiation genetics, Endothelial Cells cytology, Endothelial Cells metabolism, Lung cytology, Myocytes, Smooth Muscle cytology, Myocytes, Smooth Muscle metabolism, Side-Population Cells cytology, Side-Population Cells metabolism
- Abstract
Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrow‑derived) and a CD45‑ subset that can be subdivided into CD31‑ and CD31+ subpopulations. CD45‑/CD31‑ lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45‑/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45‑/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2‑ and VEGFR2+ cells. The CD45‑/CD31+/VEGFR2‑ LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45‑/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45‑/CD31+/VEGFR2‑ LSP cells differentiated into CD45‑/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45‑/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45‑/CD31+/VEGFR2‑ LSP cells. Taken together, the results suggest that CD45‑/CD31+ LSP cells can be separated into CD45‑/CD31+/VEGFR2‑ LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45‑/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.
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- 2019
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20. Cannabinoid-induced increase of quantal size and enhanced neuromuscular transmission.
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Morsch M, Protti DA, Cheng D, Braet F, Chung RS, Reddel SW, and Phillips WD
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- Animals, Benzoxazines pharmacology, Disease Models, Animal, Endocannabinoids metabolism, Endocannabinoids pharmacology, Evoked Potentials drug effects, Female, Mice, Mice, Inbred C57BL, Miniature Postsynaptic Potentials drug effects, Morpholines pharmacology, Myasthenia Gravis etiology, Myasthenia Gravis metabolism, Naphthalenes pharmacology, Neuromuscular Junction drug effects, Endocannabinoids administration & dosage, Myasthenia Gravis drug therapy, Neuromuscular Junction metabolism, Synaptic Transmission drug effects
- Abstract
Cannabinoids exert dynamic control over many physiological processes including memory formation, cognition and pain perception. In the central nervous system endocannabinoids mediate negative feedback of quantal transmitter release following postsynaptic depolarization. The influence of cannabinoids in the peripheral nervous system is less clear and might have broad implications for the therapeutic application of cannabinoids. We report a novel cannabinoid effect upon the mouse neuromuscular synapse: acutely increasing synaptic vesicle volume and raising the quantal amplitudes. In a mouse model of myasthenia gravis the cannabinoid receptor agonist WIN 55,212 reversed fatiguing failure of neuromuscular transmission, suggesting future therapeutic potential. Our data suggest an endogenous pathway by which cannabinoids might help to regulate transmitter release at the neuromuscular junction.
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- 2018
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21. The mouse passive-transfer model of MuSK myasthenia gravis: disrupted MuSK signaling causes synapse failure.
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Ghazanfari N, Trajanovska S, Morsch M, Liang SX, Reddel SW, and Phillips WD
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- Animals, Cholinesterase Inhibitors pharmacology, Female, Humans, Immunization, Passive, Mice, Muscle Proteins metabolism, Myasthenia Gravis, Autoimmune, Experimental immunology, Myasthenia Gravis, Autoimmune, Experimental physiopathology, Receptor Protein-Tyrosine Kinases physiology, Receptors, Cholinergic immunology, Receptors, Cholinergic metabolism, Synapses immunology, Synapses physiology, Myasthenia Gravis, Autoimmune, Experimental etiology, Receptor Protein-Tyrosine Kinases antagonists & inhibitors, Receptor Protein-Tyrosine Kinases immunology
- Abstract
While the majority of myasthenia gravis patients express antibodies targeting the acetylcholine receptor, the second most common cohort instead displays autoantibodies against muscle-specific kinase (MuSK). MuSK is a transmembrane tyrosine kinase found in the postsynaptic membrane of the neuromuscular junction. During development, MuSK serves as a signaling hub, coordinating the alignment of the pre- and postsynaptic components of the synapse. Adult mice that received repeated daily injections of IgG from anti-MuSK
+ myasthenia gravis patients developed muscle weakness, associated with neuromuscular transmission failure. MuSK autoantibodies are predominantly of the IgG4 type. They suppress the kinase activity of MuSK and the phosphorylation of target proteins in the postsynaptic membrane. Loss of postsynaptic acetylcholine receptors is the primary cause of neuromuscular transmission failure. MuSK autoantibodies also disrupt the capacity of the motor nerve terminal to adaptively increase acetylcholine release in response to the reduced postsynaptic responsiveness to acetylcholine. The passive IgG transfer model of MuSK myasthenia gravis has been used to test candidate treatments. Pyridostigmine, a first-line cholinesterase inhibitor drug, exacerbated the disease process, while 3,4-diaminopyridine and albuterol were found to be beneficial in this mouse model., (© 2017 New York Academy of Sciences.)- Published
- 2018
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22. Proliferation, differentiation and migration of SCA1 - /CD31 - cardiac side population cells in vitro and in vivo.
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Wang XZ, Gao RL, Sun P, Liu S, Xu Y, Liang DZ, Yin LM, Phillips WD, and Liang SX
- Subjects
- Animals, Cell Culture Techniques, Cell Differentiation, Cell Movement, Cell Proliferation, Disease Models, Animal, Female, Mice, Mice, Inbred C57BL, Myocardial Infarction etiology, Ataxin-1 physiology, Endothelial Cells physiology, Myocardial Infarction pathology, Myocytes, Cardiac physiology, Platelet Endothelial Cell Adhesion Molecule-1 physiology, Side-Population Cells physiology
- Abstract
Background: Side-population (SP) cells, identified by their capacity to efflux Hoechst dye, are highly enriched for stem/progenitor cell activity. They are found in many mammalian tissues, including mouse heart. Studies suggest that cardiac SP (CSP) cells can be divided into SCA1
+ /CD31- , SCA1+ /CD31+ and SCA1- /CD31- CSP subpopulations. SCA1+ /CD31- were shown to be cardiac and endothelial stem/progenitors while SCA1+ /CD31+ CSP cells are endothelial progenitors. SCA1- /CD31- CSP cells remain to be fully characterized. In this study, we characterized SCA1- /CD31- CSP cells in the adult mouse heart, and investigated their abilities to proliferate, differentiate and migrate in vitro and in vivo., Methods and Results: Using fluorescence-activated cell sorting, reverse transcriptase/polymerase chain reaction, assays of cell proliferation, differentiation and migration, and a murine model of myocardial infarction we show that SCA1- /CD31- CSP cells are located in the heart mesenchyme and express genes characteristic of stem cells and endothelial progenitors. These cells were capable of proliferation, differentiation, migration and vascularization in vitro and in vivo. Following experimental myocardial infarction, the SCA1- /CD31- CSP cells migrated from non-infarcted areas to the infarcted region within the myocardium where they differentiated into endothelial cells forming vascular (tube-like) structures. We further demonstrated that the SDF-1α/CXCR4 pathway may play an important role in migration of these cells after myocardial infarction., Conclusions: Based on their gene expression profile, localization and ability to proliferate, differentiate, migrate and vascularize in vitro and in vivo, we conclude that SCA1- /CD31- CSP cells may serve as endothelial progenitor cells in the adult mouse heart., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)- Published
- 2017
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23. Postnatal Development of Spasticity Following Transgene Insertion in the Mouse βIV Spectrin Gene (SPTBN4).
- Author
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Kichkin E, Visvanathan A, Lovicu FJ, Shu DY, Das SJ, Reddel SW, McCann EP, Zhang KY, Williams KL, Blair IP, and Phillips WD
- Subjects
- Animals, Body Weight physiology, Brain metabolism, Female, Gene Expression, Hindlimb, Male, Mice, Transgenic, Motor Endplate metabolism, Motor Endplate pathology, Muscle Spasticity pathology, Paresis metabolism, Paresis pathology, Phenotype, Spectrin genetics, Transgenes, Muscle Spasticity metabolism, Mutagenesis, Insertional, Spectrin metabolism
- Abstract
Background: The L25 mouse line was generated by random genomic insertion of a lens-specific transgene. Inbreeding of L25 hemizygotes revealed an unanticipated spastic phenotype in the hind limbs., Objective: The goals were to characterize the motor phenotype in the L25 mice and to map the transgene insert site within the mouse genome., Methods: Six pairs of L25+/- mice were repeatedly mated. Beginning at weaning, all progeny were inspected for body weight and motor signs twice weekly until they displayed predefined ethical criteria for termination. The transgene insert site was determined by whole genome sequencing. Western blotting was used to compare the expression levels of beta-IV spectrin protein in the brain., Results: Matings of hemizygous L25+/- × L25+/- mice yielded 20% (29/148) affected weanlings, identified by an abnormal retraction of the hind limbs when lifted by the tail, and a fine tremor. Affected mice were less mobile and grew more slowly than wild-type littermates. All affected mice required termination due to >15% loss of body weight (50% survival age 92 days). At the endpoint, mice showed varying degrees of spastic paresis or spastic paralysis localised to the hind limbs. Motor endplates remained fully innervated. Genome sequencing confirmed that the transgene was inserted in the locus of βIV spectrin of L25 mice. Western blotting indicated that this random insertion had greatly reduced the expression of βIV spectrin protein in the affected L25 mice., Conclusions: The results confirm the importance of βIV spectrin for maintaining central motor pathway control of the hind limbs, and provide a developmental time course for the phenotype.
- Published
- 2017
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24. Pathogenesis of myasthenia gravis: update on disease types, models, and mechanisms.
- Author
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Phillips WD and Vincent A
- Abstract
Myasthenia gravis is an autoimmune disease of the neuromuscular junction (NMJ) caused by antibodies that attack components of the postsynaptic membrane, impair neuromuscular transmission, and lead to weakness and fatigue of skeletal muscle. This can be generalised or localised to certain muscle groups, and involvement of the bulbar and respiratory muscles can be life threatening. The pathogenesis of myasthenia gravis depends upon the target and isotype of the autoantibodies. Most cases are caused by immunoglobulin (Ig)G1 and IgG3 antibodies to the acetylcholine receptor (AChR). They produce complement-mediated damage and increase the rate of AChR turnover, both mechanisms causing loss of AChR from the postsynaptic membrane. The thymus gland is involved in many patients, and there are experimental and genetic approaches to understand the failure of immune tolerance to the AChR. In a proportion of those patients without AChR antibodies, antibodies to muscle-specific kinase (MuSK), or related proteins such as agrin and low-density lipoprotein receptor-related protein 4 (LRP4), are present. MuSK antibodies are predominantly IgG4 and cause disassembly of the neuromuscular junction by disrupting the physiological function of MuSK in synapse maintenance and adaptation. Here we discuss how knowledge of neuromuscular junction structure and function has fed into understanding the mechanisms of AChR and MuSK antibodies. Myasthenia gravis remains a paradigm for autoantibody-mediated conditions and these observations show how much there is still to learn about synaptic function and pathological mechanisms.
- Published
- 2016
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25. Rifampicin-dependent antibodies target glycoprotein IIb/IIIa and cause clearance of human platelets in NOD/SCID mice.
- Author
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Sun P, Wu G, Gao RL, Liu S, Phillips WD, and Liang SX
- Subjects
- Animals, Glucocorticoids therapeutic use, Humans, Immunoglobulins, Intravenous therapeutic use, Methylprednisolone therapeutic use, Mice, Inbred NOD, Mice, SCID, Blood Platelets immunology, Platelet Glycoprotein GPIIb-IIIa Complex immunology, Purpura, Thrombocytopenic, Idiopathic immunology, Rifampin immunology
- Published
- 2016
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26. Forced expression of muscle specific kinase slows postsynaptic acetylcholine receptor loss in a mouse model of MuSK myasthenia gravis.
- Author
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Ghazanfari N, Linsao EL, Trajanovska S, Morsch M, Gregorevic P, Liang SX, Reddel SW, and Phillips WD
- Abstract
We investigated the influence of postsynaptic tyrosine kinase signaling in a mouse model of muscle-specific kinase (MuSK) myasthenia gravis (MG). Mice administered repeated daily injections of IgG from MuSK MG patients developed impaired neuromuscular transmission due to progressive loss of acetylcholine receptor (AChR) from the postsynaptic membrane of the neuromuscular junction. In this model, anti-MuSK-positive IgG caused a reduction in motor endplate immunolabeling for phosphorylated Src-Y418 and AChR β-subunit-Y390 before any detectable loss of MuSK or AChR from the endplate. Adeno-associated viral vector (rAAV) encoding MuSK fused to enhanced green fluorescent protein (MuSK-EGFP) was injected into the tibialis anterior muscle to increase MuSK synthesis. When mice were subsequently challenged with 11 daily injections of IgG from MuSK MG patients, endplates expressing MuSK-EGFP retained more MuSK and AChR than endplates of contralateral muscles administered empty vector. Recordings of compound muscle action potentials from myasthenic mice revealed less impairment of neuromuscular transmission in muscles that had been injected with rAAV-MuSK-EGFP than contralateral muscles (empty rAAV controls). In contrast to the effects of MuSK-EGFP, forced expression of rapsyn-EGFP provided no such protection to endplate AChR when mice were subsequently challenged with MuSK MG IgG. In summary, the immediate in vivo effect of MuSK autoantibodies was to suppress MuSK-dependent tyrosine phosphorylation of proteins in the postsynaptic membrane, while increased MuSK synthesis protected endplates against AChR loss. These results support the hypothesis that reduced MuSK kinase signaling initiates the progressive disassembly of the postsynaptic membrane scaffold in this mouse model of MuSK MG., (© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.)
- Published
- 2015
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27. Guidelines for pre-clinical animal and cellular models of MuSK-myasthenia gravis.
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Phillips WD, Christadoss P, Losen M, Punga AR, Shigemoto K, Verschuuren J, and Vincent A
- Subjects
- Animals, Autoantibodies immunology, Guidelines as Topic, Humans, Receptor Protein-Tyrosine Kinases immunology, Receptors, Cholinergic immunology, Myasthenia Gravis, Myasthenia Gravis, Autoimmune, Experimental, Research Design standards
- Abstract
Muscle-specific tyrosine kinase (MuSK) autoantibodies are the hallmark of a form of myasthenia gravis (MG) that can challenge the neurologist and the experimentalist. The clinical disease can be difficult to treat effectively. MuSK autoantibodies affect the neuromuscular junction in several ways. When added to muscle cells in culture, MuSK antibodies disperse acetylcholine receptor clusters. Experimental animals actively immunized with MuSK develop MuSK autoantibodies and muscle weakness. Weakness is associated with reduced postsynaptic acetylcholine receptor numbers, reduced amplitudes of miniature endplate potentials and endplate potentials, and failure of neuromuscular transmission. Similar impairments have been found in mice injected with IgG from MG patients positive for MuSK autoantibody (MuSK-MG). The active and passive models have begun to reveal the mechanisms by which MuSK antibodies disrupt synaptic function at the neuromuscular junction, and should be valuable in developing therapies for MuSK-MG. However, translation into new and improved treatments for patients requires procedures that are not too cumbersome but suitable for examining different aspects of MuSK function and the effects of potential therapies. Study design, conduct and analysis should be carefully considered and transparently reported. Here we review what has been learnt from animal and culture models of MuSK-MG, and offer guidelines for experimental design and conduct of studies, including sample size determination, randomization, outcome parameters and precautions for objective data analysis. These principles may also be relevant to the increasing number of other antibody-mediated diseases that are now recognized., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2015
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28. Electrophysiological analysis of neuromuscular synaptic function in myasthenia gravis patients and animal models.
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Plomp JJ, Morsch M, Phillips WD, and Verschuuren JJ
- Subjects
- Animals, Disease Models, Animal, Electrophysiology, Humans, Synaptic Transmission physiology, Myasthenia Gravis physiopathology, Neuromuscular Junction anatomy & histology, Neuromuscular Junction physiology
- Abstract
Study of the electrophysiological function of the neuromuscular junction (NMJ) is instrumental in the understanding of the symptoms and pathophysiology of myasthenia gravis (MG), an autoimmune disorder characterized by fluctuating and fatigable muscle weakness. Most patients have autoantibodies to the acetylcholine receptor at the NMJ. However, in recent years autoantibodies to other crucial postsynaptic membrane proteins have been found in previously 'seronegative' MG patients. Electromyographical recording of compound and single-fibre muscle action potentials provides a crucial in vivo method to determine neuromuscular transmission failure while ex vivo (miniature) endplate potential recordings can reveal the precise synaptic impairment. Here we will review these electrophysiological methods used to assess NMJ function and discuss their application and typical results found in the diagnostic and experimental study of patients and animal models of the several forms of MG., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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29. Mouse models of myasthenia gravis.
- Author
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Ban J and Phillips WD
- Subjects
- Animals, Autoantibodies immunology, Humans, Mice, Myasthenia Gravis pathology, Disease Models, Animal, Myasthenia Gravis immunology
- Abstract
Myasthenia gravis is a muscle weakness disease characterized by autoantibodies that target components of the neuromuscular junction, impairing synaptic transmission. The most common form of myasthenia gravis involves antibodies that bind the nicotinic acetylcholine receptors in the postsynaptic membrane. Many of the remaining cases are due to antibodies against muscle specific tyrosine kinase (MuSK). Recently, autoantibodies against LRP4 (another component of the MuSK signaling complex in the postsynaptic membrane) were identified as the likely cause of myasthenia gravis in some patients. Fatiguing weakness is the common symptom in all forms of myasthenia gravis, but muscles of the body are differentially affected, for reasons that are not fully understood. Much of what we have learnt about the immunological and neurobiological aspects of the pathogenesis derives from mouse models. The most widely used mouse models involve either passive transfer of autoantibodies, or active immunization of the mouse with acetylcholine receptors or MuSK protein. These models can provide a robust replication of many of the features of the human disease. Depending upon the protocol, acute fatiguing weakness develops 2 - 14 days after the start of autoantibody injections (passive transfer) or might require repeated immunizations over several weeks (active models). Here we review mouse models of myasthenia gravis, including what they have contributed to current understanding of the pathogenic mechanisms and their current application to the testing of therapeutics.
- Published
- 2015
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30. The neuromuscular junction: measuring synapse size, fragmentation and changes in synaptic protein density using confocal fluorescence microscopy.
- Author
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Tse N, Morsch M, Ghazanfari N, Cole L, Visvanathan A, Leamey C, and Phillips WD
- Subjects
- Animals, Female, Fluorescence Resonance Energy Transfer, Mice, Mice, Inbred C57BL, Motor Endplate metabolism, Neuromuscular Junction anatomy & histology, Neuromuscular Junction metabolism, Receptors, Cholinergic metabolism, Synapses metabolism, Microscopy, Confocal methods, Nerve Tissue Proteins metabolism, Neuromuscular Junction physiology, Synapses physiology
- Abstract
The neuromuscular junction (NMJ) is the large, cholinergic relay synapse through which mammalian motor neurons control voluntary muscle contraction. Structural changes at the NMJ can result in neurotransmission failure, resulting in weakness, atrophy and even death of the muscle fiber. Many studies have investigated how genetic modifications or disease can alter the structure of the mouse NMJ. Unfortunately, it can be difficult to directly compare findings from these studies because they often employed different parameters and analytical methods. Three protocols are described here. The first uses maximum intensity projection confocal images to measure the area of acetylcholine receptor (AChR)-rich postsynaptic membrane domains at the endplate and the area of synaptic vesicle staining in the overlying presynaptic nerve terminal. The second protocol compares the relative intensities of immunostaining for synaptic proteins in the postsynaptic membrane. The third protocol uses Fluorescence Resonance Energy Transfer (FRET) to detect changes in the packing of postsynaptic AChRs at the endplate. The protocols have been developed and refined over a series of studies. Factors that influence the quality and consistency of results are discussed and normative data are provided for NMJs in healthy young adult mice.
- Published
- 2014
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31. Clinical and scientific aspects of muscle-specific tyrosine kinase-related myasthenia gravis.
- Author
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Reddel SW, Morsch M, and Phillips WD
- Subjects
- Animals, Disease Models, Animal, Humans, Immunosuppression Therapy, Myasthenia Gravis epidemiology, Myasthenia Gravis immunology, Plasma Exchange, Autoantibodies blood, Myasthenia Gravis diagnosis, Myasthenia Gravis therapy, Neuromuscular Junction physiopathology, Receptor Protein-Tyrosine Kinases immunology
- Abstract
Purpose of Review: Antibodies to muscle-specific tyrosine kinase (MuSK) characterize up to 5% of myasthenia gravis patients. This review focuses on the differences to clinical antiacetylcholine receptor-myasthenia gravis, and on the physiology and animal studies that elucidate the role of MuSK and help explain the clinical disease., Recent Findings: MuSK forms the core of a protein complex in the postsynaptic membrane at the neuromuscular junction. During development, MuSK tyrosine kinase signaling is vital for the formation and stabilization of the postsynaptic endplate; it is now clear that long-term homeostasis of mature neuromuscular junctions requires MuSK function. Patient MuSK-antibodies are largely of the IgG4 type and in cell culture block the assembly and activation of MuSK kinase. Active immunization and passive transfer mouse models show reduced postsynaptic acetylcholine receptors and disturbed synaptic alignment, diminished synaptic potentials and impaired muscle activation.MuSK myasthenia gravis patients display particular bulbar and respiratory muscle involvement, with a high rate of myasthenic crises. Plasma exchange and immunosuppression with corticosteroids and rituximab appear to be most effective in treating MuSK myasthenia gravis. In contrast, the cholinesterase inhibitors, such as pyridostigmine, appear less suitable for this form of myasthenia gravis., Summary: MuSK myasthenia gravis has distinct clinical and pathophysiological features.
- Published
- 2014
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32. Muscle-specific kinase (MuSK) autoantibodies suppress the MuSK pathway and ACh receptor retention at the mouse neuromuscular junction.
- Author
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Ghazanfari N, Morsch M, Reddel SW, Liang SX, and Phillips WD
- Subjects
- Animals, Female, Humans, Mice, Mice, Inbred C57BL, Motor Endplate drug effects, Motor Endplate physiology, Muscle Proteins metabolism, Myasthenia Gravis immunology, Protein Transport, Receptor Protein-Tyrosine Kinases immunology, src-Family Kinases metabolism, Autoantibodies pharmacology, Immunoglobulin G pharmacology, Motor Endplate metabolism, Receptor Protein-Tyrosine Kinases metabolism, Receptors, Cholinergic metabolism
- Abstract
Muscle-specific kinase (MuSK) autoantibodies from myasthenia gravis patients can block the activation of MuSK in vitro and/or reduce the postsynaptic localization of MuSK. Here we use a mouse model to examine the effects of MuSK autoantibodies upon some key components of the postsynaptic MuSK pathway and upon the regulation of junctional ACh receptor (AChR) numbers. Mice became weak after 14 daily injections of anti-MuSK-positive patient IgG. The intensity and area of AChR staining at the motor endplate was markedly reduced. Pulse-labelling of AChRs revealed an accelerated loss of pre-existing AChRs from postsynaptic AChR clusters without a compensatory increase in incorporation of (newly synthesized) replacement AChRs. Large, postsynaptic AChR clusters were replaced by a constellation of tiny AChR microaggregates. Puncta of AChR staining also appeared in the cytoplasm beneath the endplate. Endplate staining for MuSK, activated Src, rapsyn and AChR were all reduced in intensity. In the tibialis anterior muscle there was also evidence that phosphorylation of the AChR β-subunit-Y390 was reduced at endplates. In contrast, endplate staining for β-dystroglycan (through which rapsyn couples AChR to the synaptic basement membrane) remained intense. The results suggest that anti-MuSK IgG suppresses the endplate density of MuSK, thereby down-regulating MuSK signalling activity and the retention of junctional AChRs locally within the postsynaptic membrane scaffold., (© 2014 The Authors. The Journal of Physiology © 2014 The Physiological Society.)
- Published
- 2014
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33. Hysteresis in a quantized superfluid 'atomtronic' circuit.
- Author
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Eckel S, Lee JG, Jendrzejewski F, Murray N, Clark CW, Lobb CJ, Phillips WD, Edwards M, and Campbell GK
- Abstract
Atomtronics is an emerging interdisciplinary field that seeks to develop new functional methods by creating devices and circuits where ultracold atoms, often superfluids, have a role analogous to that of electrons in electronics. Hysteresis is widely used in electronic circuits-it is routinely observed in superconducting circuits and is essential in radio-frequency superconducting quantum interference devices. Furthermore, it is as fundamental to superfluidity (and superconductivity) as quantized persistent currents, critical velocity and Josephson effects. Nevertheless, despite multiple theoretical predictions, hysteresis has not been previously observed in any superfluid, atomic-gas Bose-Einstein condensate. Here we directly detect hysteresis between quantized circulation states in an atomtronic circuit formed from a ring of superfluid Bose-Einstein condensate obstructed by a rotating weak link (a region of low atomic density). This contrasts with previous experiments on superfluid liquid helium where hysteresis was observed directly in systems in which the quantization of flow could not be observed, and indirectly in systems that showed quantized flow. Our techniques allow us to tune the size of the hysteresis loop and to consider the fundamental excitations that accompany hysteresis. The results suggest that the relevant excitations involved in hysteresis are vortices, and indicate that dissipation has an important role in the dynamics. Controlled hysteresis in atomtronic circuits may prove to be a crucial feature for the development of practical devices, just as it has in electronic circuits such as memories, digital noise filters (for example Schmitt triggers) and magnetometers (for example superconducting quantum interference devices).
- Published
- 2014
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34. Effects of the ß2-adrenoceptor agonist, albuterol, in a mouse model of anti-MuSK myasthenia gravis.
- Author
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Ghazanfari N, Morsch M, Tse N, Reddel SW, and Phillips WD
- Subjects
- Animals, Autoantibodies immunology, Female, Humans, Mice, Muscle, Skeletal immunology, Muscle, Skeletal pathology, Myasthenia Gravis, Autoimmune, Experimental chemically induced, Myasthenia Gravis, Autoimmune, Experimental immunology, Myasthenia Gravis, Autoimmune, Experimental pathology, Neuromuscular Junction immunology, Neuromuscular Junction pathology, Receptor Protein-Tyrosine Kinases immunology, Adrenergic beta-2 Receptor Agonists pharmacology, Albuterol pharmacology, Autoantibodies toxicity, Myasthenia Gravis, Autoimmune, Experimental drug therapy
- Abstract
The β2-adrenergic receptor agonist, albuterol, has been reported beneficial in treating several forms of congenital myasthenia. Here, for the first time, we examined the potential benefit of albuterol in a mouse model of anti-Muscle Specific Kinase (MuSK) myasthenia gravis. Mice received 15 daily injections of IgG from anti-MuSK positive patients, which resulted in whole-body weakness. At neuromuscular junctions in the tibialis anterior and diaphragm muscles the autoantibodies caused loss of postsynaptic acetylcholine receptors, and reduced the amplitudes of the endplate potential and spontaneous miniature endplate potential in the diaphragm muscle. Treatment with albuterol (8 mg/kg/day) during the two-week anti-MuSK injection series reduced the degree of weakness and weight loss, compared to vehicle-treated mice. However, the compound muscle action potential recorded from the gastrocnemius muscle displayed a decremental response in anti-MuSK-injected mice whether treated with albuterol or vehicle. Ongoing albuterol treatment did not increase endplate potential amplitudes compared to vehicle-treated mice nor did it prevent the loss of acetylcholine receptors from motor endplates. On the other hand, albuterol treatment significantly reduced the degree of fragmentation of endplate acetylcholine receptor clusters and increased the extent to which the remaining receptor clusters were covered by synaptophysin-stained nerve terminals. The results provide the first evidence that short-term albuterol treatment can ameliorate weakness in a robust mouse model of anti-MuSK myasthenia gravis. The results also demonstrate that it is possible for albuterol treatment to reduce whole-body weakness without necessarily reversing myasthenic impairment to the structure and function of the neuromuscular junction.
- Published
- 2014
- Full Text
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35. Sequence of age-associated changes to the mouse neuromuscular junction and the protective effects of voluntary exercise.
- Author
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Cheng A, Morsch M, Murata Y, Ghazanfari N, Reddel SW, and Phillips WD
- Subjects
- Age Factors, Animals, Female, Mice, Motor Neurons physiology, Muscle, Skeletal physiology, Nerve Endings, Neuromuscular Junction metabolism, Neuromuscular Junction pathology, Receptors, Cholinergic metabolism, Synaptophysin metabolism, Aging physiology, Neuromuscular Junction physiology, Physical Conditioning, Animal
- Abstract
Loss of connections between motor neurons and skeletal muscle fibers contribute to motor impairment in old age, but the sequence of age-associated changes that precede loss of the neuromuscular synapse remains uncertain. Here we determine changes in the size of neuromuscular synapses within the tibialis anterior muscle across the life span of C57BL/6J mice. Immunofluorescence, confocal microscopy and morphometry were used to measure the area occupied by nerve terminal synaptophysin staining and postsynaptic acetylcholine receptors at motor endplates of 2, 14, 19, 22, 25 and 28 month old mice. The key findings were: 1) At middle age (14-months) endplate acetylcholine receptors occupied 238 ± 11 µm(2) and nerve terminal synaptophysin 168 ± 14 µm(2) (mean ± SEM). 2) Between 14-months and 19-months (onset of old age) the area occupied by postsynaptic acetylcholine receptors declined 30%. At many endplates the large acetylcholine receptor plaque became fragmented into multiple smaller acetylcholine receptor clusters. 3) Between 19- and 25-months, the fraction of endplate acetylcholine receptors covered by synaptophysin fell 21%. By 28-months, half of the endplates imaged retained ≤ 50 µm(2) area of synaptophysin staining. 4) Within aged muscles, the degree to which an endplate remained covered by synaptophysin did not depend upon the total area of acetylcholine receptors, nor upon the number of discrete receptor clusters. 5) Voluntary wheel-running exercise, beginning late in middle-age, prevented much of the age-associated loss of nerve terminal synaptophysin. In summary, a decline in the area of endplate acetylcholine receptor clusters at the onset of old age was followed by loss of nerve terminal synaptophysin from the endplate. Voluntary running exercise, begun late in middle age, substantially inhibited the loss of nerve terminal from aging motor endplates.
- Published
- 2013
- Full Text
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36. Pyridostigmine but not 3,4-diaminopyridine exacerbates ACh receptor loss and myasthenia induced in mice by muscle-specific kinase autoantibody.
- Author
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Morsch M, Reddel SW, Ghazanfari N, Toyka KV, and Phillips WD
- Subjects
- 4-Aminopyridine analogs & derivatives, 4-Aminopyridine pharmacology, Amifampridine, Animals, Autoantibodies pharmacology, Cholinesterase Inhibitors pharmacology, Evoked Potentials, Female, Humans, Immunoglobulin G pharmacology, Mice, Mice, Inbred C57BL, Muscle, Skeletal physiology, Neuromuscular Junction drug effects, Neuromuscular Junction physiology, Pyridostigmine Bromide pharmacology, Muscle Weakness physiopathology, Myasthenia Gravis, Autoimmune, Experimental physiopathology, Receptor Protein-Tyrosine Kinases physiology, Receptors, Cholinergic physiology
- Abstract
In myasthenia gravis, the neuromuscular junction is impaired by the antibody-mediated loss of postsynaptic acetylcholine receptors (AChRs). Muscle weakness can be improved upon treatment with pyridostigmine, a cholinesterase inhibitor, or with 3,4-diaminopyridine, which increases the release of ACh quanta. The clinical efficacy of pyridostigmine is in doubt for certain forms of myasthenia. Here we formally examined the effects of these compounds in the antibody-induced mouse model of anti-muscle-specific kinase (MuSK) myasthenia gravis. Mice received 14 daily injections of IgG from patients with anti-MuSK myasthenia gravis. This caused reductions in postsynaptic AChR densities and in endplate potential amplitudes. Systemic delivery of pyridostigmine at therapeutically relevant levels from days 7 to 14 exacerbated the anti-MuSK-induced structural alterations and functional impairment at motor endplates in the diaphragm muscle. No such effect of pyridostigmine was found in mice receiving control human IgG. Mice receiving smaller amounts of MuSK autoantibodies did not display overt weakness, but 9 days of pyridostigmine treatment precipitated generalised muscle weakness. In contrast, one week of treatment with 3,4-diaminopyridine enhanced neuromuscular transmission in the diaphragm muscle. Both pyridostigmine and 3,4-diaminopyridine increase ACh in the synaptic cleft yet only pyridostigmine potentiated the anti-MuSK-induced decline in endplate ACh receptor density. These results thus suggest that ongoing pyridostigmine treatment potentiates anti-MuSK-induced AChR loss by prolonging the activity of ACh in the synaptic cleft.
- Published
- 2013
- Full Text
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37. Profile of David Wineland and Serge Haroche, 2012 Nobel laureates in physics.
- Author
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Phillips WD
- Subjects
- History, 20th Century, History, 21st Century, Quantum Theory, United States, Nobel Prize, Physics history
- Published
- 2013
- Full Text
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38. Migration of resident cardiac stem cells in myocardial infarction.
- Author
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Liang SX and Phillips WD
- Subjects
- Animals, Biomarkers metabolism, Cell Lineage, Humans, Myocardial Infarction metabolism, Myocardium metabolism, Phenotype, Signal Transduction, Stem Cells metabolism, Cell Movement, Myocardial Infarction pathology, Myocardium pathology, Stem Cells pathology
- Abstract
Ischemic heart disease is a major cause of morbidity and mortality worldwide. Stem cell-based therapy, which aims to restore cardiac structure and function by regeneration of functional myocardium, has recently been proposed as a novel alternative treatment modality. Resident cardiac stem cells (CSCs) in adult hearts are a key cell type under investigation. CSCs have been shown to be able to repair damaged myocardium and improve myocardial function in both human and animal studies. This approach relies not only on the proliferation of the CSCs, but also upon their migration to the site of injury within the heart. Here, we briefly review reported CSC populations and discuss signaling factors and pathways required for the migration of CSCs., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2013
- Full Text
- View/download PDF
39. Driving phase slips in a superfluid atom circuit with a rotating weak link.
- Author
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Wright KC, Blakestad RB, Lobb CJ, Phillips WD, and Campbell GK
- Abstract
We have observed well-defined phase slips between quantized persistent current states around a toroidal atomic (23Na) Bose-Einstein condensate. These phase slips are induced by a weak link (a localized region of reduced superfluid density) rotated slowly around the ring. This is analogous to the behavior of a superconducting loop with a weak link in the presence of an external magnetic field. When the weak link is rotated more rapidly, well-defined phase slips no longer occur, and vortices enter into the bulk of the condensate. A noteworthy feature of this system is the ability to dynamically vary the current-phase relation of the weak link, a feature which is difficult to implement in superconducting or superfluid helium circuits.
- Published
- 2013
- Full Text
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40. Muscle specific kinase autoantibodies cause synaptic failure through progressive wastage of postsynaptic acetylcholine receptors.
- Author
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Morsch M, Reddel SW, Ghazanfari N, Toyka KV, and Phillips WD
- Subjects
- Action Potentials physiology, Animals, Autoantigens immunology, Electromyography, Female, Mice, Mice, Inbred C57BL, Microscopy, Confocal, Myasthenia Gravis immunology, Myasthenia Gravis physiopathology, Neuromuscular Junction immunology, Neuromuscular Junction physiopathology, Synapses, Synaptic Transmission physiology, Autoantibodies immunology, Myasthenia Gravis metabolism, Neuromuscular Junction metabolism, Receptor Protein-Tyrosine Kinases immunology, Receptors, Cholinergic metabolism
- Abstract
In myasthenia gravis muscle weakness is caused by autoantibodies against components of the neuromuscular junction. Patient autoantibodies against muscle specific kinase (MuSK) deplete MuSK from the postsynaptic membrane and reproduce signs of myasthenia gravis when injected into mice. Here we have examined the time-course of structural and functional changes that lead up to synaptic failure. C57Bl6J mice received daily injections of anti-MuSK patient IgG for 15 days. Mice began to lose weight from day 12 and demonstrated whole-body weakness by day 14. Electromyography indicated synaptic impairment from day 6 in the gastrocnemius muscle and from day 10 in the diaphragm muscle. Confocal microscopy revealed linear declines in the area and density of postsynaptic acetylcholine receptors (3-5% per day) from day 1 through day 15 of the injection series in all five muscles examined. Intracellular recordings from the diaphragm muscle revealed comparable progressive declines in the amplitude of the endplate potential and miniature endplate potential of 3-4% per day. Neither quantal content nor the postsynaptic action potential threshold changed significantly over the injection series. The inverse relationship between the quantal amplitude of a synapse and its quantal content disappeared only late in the injection series (day 10). Our results suggest that the primary myasthenogenic action of anti-MuSK IgG is to cause wastage of postsynaptic acetylcholine receptor density. Consequent reductions in endplate potential amplitudes culminated in failure of neuromuscular transmission., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
41. Partial-transfer absorption imaging: a versatile technique for optimal imaging of ultracold gases.
- Author
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Ramanathan A, Muniz SR, Wright KC, Anderson RP, Phillips WD, Helmerson K, and Campbell GK
- Abstract
Partial-transfer absorption imaging is a tool that enables optimal imaging of atomic clouds for a wide range of optical depths. In contrast to standard absorption imaging, the technique can be minimally destructive and can be used to obtain multiple successive images of the same sample. The technique involves transferring a small fraction of the sample from an initial internal atomic state to an auxiliary state and subsequently imaging that fraction absorptively on a cycling transition. The atoms remaining in the initial state are essentially unaffected. We demonstrate the technique, discuss its applicability, and compare its performance as a minimally destructive technique to that of phase-contrast imaging.
- Published
- 2012
- Full Text
- View/download PDF
42. Observation of a superfluid Hall effect.
- Author
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LeBlanc LJ, Jiménez-García K, Williams RA, Beeler MC, Perry AR, Phillips WD, and Spielman IB
- Subjects
- Electric Conductivity, Electrons, Evaluation Studies as Topic, Hydrodynamics, Cold Temperature, Magnetics methods, Quantum Theory, Semiconductors
- Abstract
Measurement techniques based upon the Hall effect are invaluable tools in condensed-matter physics. When an electric current flows perpendicular to a magnetic field, a Hall voltage develops in the direction transverse to both the current and the field. In semiconductors, this behavior is routinely used to measure the density and charge of the current carriers (electrons in conduction bands or holes in valence bands)--internal properties of the system that are not accessible from measurements of the conventional resistance. For strongly interacting electron systems, whose behavior can be very different from the free electron gas, the Hall effect's sensitivity to internal properties makes it a powerful tool; indeed, the quantum Hall effects are named after the tool by which they are most distinctly measured instead of the physics from which the phenomena originate. Here we report the first observation of a Hall effect in an ultracold gas of neutral atoms, revealed by measuring a Bose-Einstein condensate's transport properties perpendicular to a synthetic magnetic field. Our observations in this vortex-free superfluid are in good agreement with hydrodynamic predictions, demonstrating that the system's global irrotationality influences this superfluid Hall signal.
- Published
- 2012
- Full Text
- View/download PDF
43. Neuregulin-1 potentiates agrin-induced acetylcholine receptor clustering through muscle-specific kinase phosphorylation.
- Author
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Ngo ST, Cole RN, Sunn N, Phillips WD, and Noakes PG
- Subjects
- Animals, Cell Differentiation physiology, Cell Line, Female, Humans, Mice, Mice, Inbred C57BL, Myoblasts cytology, Myoblasts enzymology, Phosphorylation physiology, Pregnancy, Primary Cell Culture, Rats, Agrin physiology, Neuregulin-1 physiology, Receptor Protein-Tyrosine Kinases metabolism, Receptors, Cholinergic metabolism
- Abstract
At neuromuscular synapses, neural agrin (n-agrin) stabilizes embryonic postsynaptic acetylcholine receptor (AChR) clusters by signalling through the muscle-specific kinase (MuSK) complex. Live imaging of cultured myotubes showed that the formation and disassembly of primitive AChR clusters is a dynamic and reversible process favoured by n-agrin, and possibly other synaptic signals. Neuregulin-1 is a growth factor that can act through muscle ErbB receptor kinases to enhance synaptic gene transcription. Recent studies suggest that neuregulin-1-ErbB signalling can modulate n-agrin-induced AChR clustering independently of its effects on transcription. Here we report that neuregulin-1 increased the size of developing AChR clusters when injected into muscles of embryonic mice. We investigated this phenomenon using cultured myotubes, and found that in the ongoing presence of n-agrin, neuregulin-1 potentiates AChR clustering by increasing the tyrosine phosphorylation of MuSK. This potentiation could be blocked by inhibiting Shp2, a postsynaptic tyrosine phosphatase known to modulate the activity of MuSK. Our results provide new evidence that neuregulin-1 modulates the signaling activity of MuSK and hence might function as a second-order regulator of postsynaptic AChR clustering at the neuromuscular synapse. Thus two classic synaptic signalling systems (neuregulin-1 and n-agrin) converge upon MuSK to regulate postsynaptic differentiation.
- Published
- 2012
- Full Text
- View/download PDF
44. Synthetic partial waves in ultracold atomic collisions.
- Author
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Williams RA, LeBlanc LJ, Jiménez-García K, Beeler MC, Perry AR, Phillips WD, and Spielman IB
- Abstract
Interactions between particles can be strongly altered by their environment. We demonstrate a technique for modifying interactions between ultracold atoms by dressing the bare atomic states with light, creating an effective interaction of vastly increased range that scatters states of finite relative angular momentum at collision energies where only s-wave scattering would normally be expected. We collided two optically dressed neutral atomic Bose-Einstein condensates with equal, and opposite, momenta and observed that the usual s-wave distribution of scattered atoms was altered by the appearance of d- and g-wave contributions. This technique is expected to enable quantum simulation of exotic systems, including those predicted to support Majorana fermions.
- Published
- 2012
- Full Text
- View/download PDF
45. Caveolae respond to cell stretch and contribute to stretch-induced signaling.
- Author
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Gervásio OL, Phillips WD, Cole L, and Allen DG
- Subjects
- Animals, Biomechanical Phenomena, Caveolin 3 genetics, Caveolin 3 metabolism, Cell Size, Fluorescence Resonance Energy Transfer, Mice, Myoblasts cytology, Myoblasts metabolism, Caveolae chemistry, Caveolae metabolism, Myoblasts chemistry, Signal Transduction
- Abstract
Caveolae are invaginations of the plasma membrane that are formed by caveolins. Caveolar membranes are also enriched in cholesterol, glycosphingolipids and signaling enzymes such as Src kinase. Here we investigate the effect of cell stretch upon caveolar dynamics and signaling. Transfection of C2 myoblasts with caveolin-3-YFP led to the formation of caveolae-like membrane pits 50-100 nm in diameter. Glycosphingolipids became immobilized and tightly packed together within caveolin-rich regions of the plasma membrane. Fluorescence resonance energy transfer (FRET) was used to assess the degree of glycosphingolipid packing. Myoblasts were subjected to a brief (1 minute) stretch on an elastic substratum. Stretch caused a reduction in glycosphingolipid FRET, consistent with a reversible unfolding of caveolar pits in response to membrane tension. Cells expressing caveolin-3-YFP also displayed an enhanced stretch-induced activation of Src kinase, as assessed by immunofluorescence. Repeated stretches resulted in the trafficking and remodeling of caveolin-3-rich membrane domains and accelerated turnover of membrane glycosphingolipids. The stretch-induced unfolding of caveolae, activation of Src and redistribution of caveolin and glycosphingolipids might reflect mechanisms of the cellular adaptation to mechanical stresses.
- Published
- 2011
- Full Text
- View/download PDF
46. Ultracold atoms and precise time standards.
- Author
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Campbell GK and Phillips WD
- Abstract
Experimental techniques of laser cooling and trapping, along with other cooling techniques, have produced gaseous samples of atoms so cold that they are, for many practical purposes, in the quantum ground state of their centre-of-mass motion. Such low velocities have virtually eliminated effects such as Doppler shifts, relativistic time dilation and observation-time broadening that previously limited the performance of atomic frequency standards. Today, the best laser-cooled, caesium atomic fountain, microwave frequency standards realize the International System of Units (SI) definition of the second to a relative accuracy of ≈3×10(-16). Optical frequency standards, which do not realize the SI second, have even better performance: cold neutral atoms trapped in optical lattices now yield relative systematic uncertainties of ≈1×10(-16), whereas cold-trapped ions have systematic uncertainties of 9×10(-18). We will discuss the current limitations in the performance of neutral atom atomic frequency standards and prospects for the future.
- Published
- 2011
- Full Text
- View/download PDF
47. Pulsed Sisyphus scheme for laser cooling of atomic (anti)hydrogen.
- Author
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Wu S, Brown RC, Phillips WD, and Porto JV
- Abstract
We propose a laser cooling technique in which atoms are selectively excited to a dressed metastable state whose light shift and decay rate are spatially correlated for Sisyphus cooling. The case of cooling magnetically trapped (anti)hydrogen with the 1S-2S-3P transitions by using pulsed ultraviolet and continuous-wave visible lasers is numerically simulated. We find a number of appealing features including rapid three-dimensional cooling from ∼1 K to recoil-limited, millikelvin temperatures, as well as suppressed spin-flip loss and manageable photoionization loss., (© 2011 American Physical Society)
- Published
- 2011
- Full Text
- View/download PDF
48. Superflow in a toroidal Bose-Einstein condensate: an atom circuit with a tunable weak link.
- Author
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Ramanathan A, Wright KC, Muniz SR, Zelan M, Hill WT 3rd, Lobb CJ, Helmerson K, Phillips WD, and Campbell GK
- Abstract
We have created a long-lived (≈40 s) persistent current in a toroidal Bose-Einstein condensate held in an all-optical trap. A repulsive optical barrier across one side of the torus creates a tunable weak link in the condensate circuit, which can affect the current around the loop. Superflow stops abruptly at a barrier strength such that the local flow velocity at the barrier exceeds a critical velocity. The measured critical velocity is consistent with dissipation due to the creation of vortex-antivortex pairs. This system is the first realization of an elementary closed-loop atom circuit., (© 2011 American Physical Society)
- Published
- 2011
- Full Text
- View/download PDF
49. Muscle specific kinase: organiser of synaptic membrane domains.
- Author
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Ghazanfari N, Fernandez KJ, Murata Y, Morsch M, Ngo ST, Reddel SW, Noakes PG, and Phillips WD
- Subjects
- Animals, Autoimmune Diseases enzymology, Humans, Organ Specificity, Receptor Protein-Tyrosine Kinases chemistry, Membrane Microdomains metabolism, Muscles enzymology, Receptor Protein-Tyrosine Kinases metabolism, Synaptic Membranes metabolism
- Abstract
Muscle Specific Kinase (MuSK) is a transmembrane tyrosine kinase vital for forming and maintaining the mammalian neuromuscular junction (NMJ: the synapse between motor nerve and skeletal muscle). MuSK expression switches on during skeletal muscle differentiation. MuSK then becomes restricted to the postsynaptic membrane of the NMJ, where it functions to cluster acetylcholine receptors (AChRs). The expression, activation and turnover of MuSK are each regulated by signals from the motor nerve terminal. MuSK forms the core of an emerging signalling complex that can be acutely activated by neural agrin (N-agrin), a heparin sulfate proteoglycan secreted from the nerve terminal. MuSK activation initiates complex intracellular signalling events that coordinate the local synthesis and assembly of synaptic proteins. The importance of MuSK as a synapse organiser is highlighted by cases of autoimmune myasthenia gravis in which MuSK autoantibodies can deplete MuSK from the postsynaptic membrane, leading to complete disassembly of the adult NMJ., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
50. Phases of a two-dimensional bose gas in an optical lattice.
- Author
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Jiménez-García K, Compton RL, Lin YJ, Phillips WD, Porto JV, and Spielman IB
- Abstract
Ultracold atoms in optical lattices realize simple condensed matter models. We create an ensemble of ≈60 harmonically trapped 2D Bose-Hubbard systems from a 87Rb Bose-Einstein condensate in an optical lattice and use a magnetic resonance imaging approach to select a few 2D systems for study, thereby eliminating ensemble averaging. Our identification of the transition from superfluid to Mott insulator, as a function of both atom density and lattice depth, is in excellent agreement with a universal state diagram [M. Rigol, Phys. Rev. A 79 053605 (2009)] suitable for our trapped system. In agreement with theory, our data suggest a failure of the local density approximation in the transition region.
- Published
- 2010
- Full Text
- View/download PDF
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