1. Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
- Author
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Massimo Tacchini, Ferruccio Poli, Alessandra Guerrini, Patrizia Hrelia, Gianni Sacchetti, Carmela Fimognari, Maurizio Brigotti, Manuela Mandrone, Eleonora Turrini, Francesca Maffei, Elena Catanzaro, Valentina Pellicioni, Piero Sestili, Guglielmo Paganetto, Lorenzo Ferruzzi, and Turrini E, Catanzaro E, Ferruzzi L, Guerrini A, Tacchini M, Sacchetti G, Paganetto G, Maffei F, Pellicioni V, Poli F, Hrelia P, Mandrone M, Sestili P, Brigotti M, Fimognari C.
- Subjects
0301 basic medicine ,Antineoplastic Agents, PhytogenicCaco-2 Cells, Cell Differentiation, Cell Proliferation, Cell Survival, Gene Expression Regulation, Neoplastic, Hemidesmus, Humans, Intestinal Absorption, Jurkat Cells, MicroRNAs, Plant Extracts, Plant Roots, Proteasome Endopeptidase Complex, Proteasome Inhibitors, Reactive Oxygen Species ,lcsh:Medicine ,Pharmacology ,Jurkat cells ,Plant Roots ,Intestinal absorption ,Jurkat Cells ,0302 clinical medicine ,intestinal epithelium model ,Leukaemia ,Cytotoxicity ,lcsh:Science ,chemistry.chemical_classification ,Hemidesmus ,Multidisciplinary ,biology ,Cell Differentiation ,Intestinal epithelium ,CANCER ,Gene Expression Regulation, Neoplastic ,leukemia cell ,Proteasome Inhibitors ,Proteasome Endopeptidase Complex ,Cell Survival ,NOXA ,BORTEZOMIB ,Antineoplastic Agents ,CELL DEATH ,Article ,NO ,Hemidesmus indicus ,03 medical and health sciences ,Chemotherapy ,Humans ,Cell Proliferation ,Reactive oxygen species ,Neoplastic ,Proteasome ,Plant Extracts ,lcsh:R ,Hemidesmus indicu ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,PhytogenicCaco-2 Cells ,MicroRNAs ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,Intestinal Absorption ,Apoptosis ,lcsh:Q ,Caco-2 Cells ,MCL-1 ,Reactive Oxygen Species ,030217 neurology & neurosurgery - Abstract
Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus’s phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area.
- Published
- 2019
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