12 results on '"Pilli, Hema Prakash Kumari"'
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2. Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study
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Datla, Venkata Divya Durga, primary, Uppalapati, Lakshman Varma, additional, Pilli, Hema Prakash Kumari, additional, Mandava, Jyothi, additional, Kantheti, Sirisha, additional, Komireddy, Sri Naagaja Krishnaveni, additional, and Chandolu, Vedamani, additional
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- 2024
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3. Study on Catheter-Associated Urinary Tract Infection (CAUTI): Incidence and Microbiological Profile in a Tertiary Care Hospital in Andhra Pradesh
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Hanumantha, Sreedevi, primary and Pilli, Hema Prakash Kumari, additional
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- 2021
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4. ISOLATION OF MULTI DRUG RESISTANT BACTERIA FROM BORE WATER SAMPLES IN AND AROUND VISAKHAPATNAM
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TIRUVAIPATI SIRISHA, SWAPNA SASAPU, PAYALA VIJAYALAKSHMI, and PILLI HEMA PRAKASH KUMARI
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Pharmaceutical Science - Abstract
Objective: The aim of the present study was to determine the levels of groundwater contamination by isolating total coliforms, fecal coliforms, and bacterial pathogens. Methods: A total of 32 water samples, comprising 5 samples from each GVMC urban area zone, Visakhapatnam were randomly collected from different areas. Evaluation of Total and fecal coliform counts was done by using multiple-tube fermentation technique and were reported as the most probable number (MPN) of organisms present per 100 ml of sample. Results: The samples collected from the zones Yendada (zone I), Marripalem (zone IV), Chinna musidiwada (zone VI) showed the highest total and fecal coliform counts (MPN>180). Nearly 30 samples (94%) yielded bacterial isolates. Among the isolates Pseudomonas spp. showed highest susceptibility to cefepime and imipenem antibiotics. All the microorganisms isolated were multidrug resistant with a multidrug antibiotic resistant index more than 0.2. Conclusion: These multidrug-resistant (MDR) organisms are considered as a major threat to the public health and should receive more attention.
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- 2023
5. Bacterial vaginosis and antibacterial susceptibility pattern of asymptomatic urinary tract infection in pregnant women at a tertiary care hospital, Visakhaptn, India
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Appikatla Madhu Bhavana, Pilli Hema Prakash Kumari, Nitin Mohan, Vijayalakshmai Chandrasekhar, Payala Vijayalakshmi, and Rongala Venkata Manasa
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Antibiotic susceptibility pattern ,Bacterial vaginosis ,Pregnant women ,Urinary tract infection ,Microbiology ,QR1-502 - Abstract
Background and Objectives: The association between bacterial vaginosis and urinary tract infection (UTI) in pregnant women is at a greater risk comparatively than patients with bacterial vaginosis or UTI. Bacterial vaginosis and asymptomatic UTI both pose risk for mother and fetus. Early diagnosis and treatment can save the life of both. The present investigation was aimed to find out the magnitude of asymptomatic bacteriuria in pregnant women with noticeable bacterial vaginitis attending antenatal outpatient and inpatient of a tertiary care hospital and to identify the organisms causing it. Materials and Methods: A total of 117 antenatal women from different age and parity groups with different gestational ages were included in the study. The samples were subjected to standard microbiological techniques for identification of microorganisms. While performing Per speculum examination, vaginal secretions were collected from the posterior fornix. Swabs from the posterior fornix were tested for pH using litmus paper. A wet mount and Gram smear was made and examined for the presence of bacteria, polymorphs and clue cells indicating bacterial vaginosis. Amsel’s criteria and Nugent scoring system were applied for diagnosis of bacterial vaginosis. Antibiotic susceptibility of the isolated bacteria was performed using Kirby-Bauer method. Results: Bacterial vaginosis infection rate (62.3%) was common in the present study followed by asymptomatic UTI (n=60, 51%). It was also observed that asymptomatic urinary tract infection (UTI) with Bacterial vaginosis prevalent rate was 49 (41.8%) in the current study. Conclusion: Bacterial vaginosis was more common than asymptomatic bacteriuria in pregnant women. It is recommended that antenatal health care facilities should incorporate screening of vaginitis among pregnant women to prevent the complications of pregnancy. And those women with Bacterial vaginosis should be screened for UTI. Proper use of antibiotics should be encouraged, abuse of antibiotics should be in check.
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- 2020
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6. Evaluation of microbiological profile and antibiogram of aerobic bacteria isolated from pus samples
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Pilli Hema Prakash Kumari, Purimitla Usha Rani, and Payala Vijaya Lakshmi
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Antibiotic susceptibility ,Carbapenemase production ,ESBL detection ,Multidrug resistance ,Wound infection ,Medicine - Abstract
Purulent wound infections are quite complicated to manage because of multidrug resistant bacteria. The main purpose of the present study was to assess the prevalence, microbiological profile and antibiograms of aerobic bacteria isolated from pus samples. One hundred pus sam-ples sent to the microbiology laboratory from surgical departments over a period of six months were analyzed. Isolation and detection of culture isolates was done by using standard bacteriological techniques and antibiotic susceptibility testing was performed by disc diffusion method by following CLSI guidelines on Muller-Hinton (MH) agar. Highest number of pus samples were from incision and drainage (23.8%) followed by chronic non-healing ulcer (19.04%). Twenty one different bacterial isolates were obtained from one hundred pus samples. S. aureus was the predominant bacteria (28.5%) followed by coagulase-negative Staphylococci (23.8%). The results of the antibiotics susceptibility testing illustrated that majority of the isolated organisms were MDR. S. aureus showed highest sensitivity to antibiotics like linezolid (83.3%) and teicoplanin (50%). Among the five isolated strains of coagulase-negative Staphylococci (CONS), three of them were MDR and the other two showed sensitivity to antibiotics cefaperazone, co-trimoxazole and ticarcillin/clavulanic acid (20%). Among the 9 isolates of Enterobacteriaceae, one isolate of E. coli (11.1%), two isolates of Klebsiella species (22.2%) showed ESBL pro-duction. The isolated four strains of P. aeruginosa showed ESBL pro-duction (44.4%) by CAZ/CA antibiotic susceptibility testing. These or-ganisms were screened for carbapenemase production through Modified Hodge test, 5 strains possess carbapenemase enzyme pro-duction (71.4%) and thus acquire resistance to carbapenem antibiotics. Carbapenemase production was not detected in the 2 isolates of P. aeruginosa among the 4 tested strains. The overall results showed that 85% of the isolated strains showed MDR to certain classes of antibiotics. [J Med Allied Sci 2018; 8(1.000): 26-35]
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- 2018
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7. Point Prevalence of Hepatitis B Virus Infection among Adolescents in Visakhapatnam, India
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Vijayalakshmi Payala, Sreedevi Hanumantha, and Pilli Hema Prakash Kumari
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hepatitis b virus ,viral dna ,hbsag ,pcr ,Pathology ,RB1-214 - Abstract
Introduction: Occurrence of detectable amounts of viral antigen or viral particles in the blood of infected patients with Hepatitis B virus (HBV) is a significant characteristic of HBV infection. Detection of HBV antigen or its DNA among individuals of a community is a crucial factor to know the burden of HBV infection. Hepatitis B surface antigen (HBsAg) is a suitable marker of HBV infection but a poor indicator of infectivity since its presence is not a direct measure of the presence of viable virions. Hence the tests for the detection of HBV DNA or HBsAg are used. The measurement of HBV DNA in serum has become the main tool to identify viral load, to monitor patients’ therapy and to predict whether antiviral therapy would be successful or not. Methods: The present study was designated to identify HBV infected individuals among adolescent age group by using combined methods namely, rapid immunoassay technique for HBsAg detection (HEPACARD) and Conventional Polymerase Chain reaction (PCR) analysis for HBV DNA detection. Results: Serum samples from 39 patients suspected of HBV infection were tested for the presence of HBsAg and HBV DNA. Eight specimens (20%) were positive for HBsAg as well as HBV DNA using PCR reaction. The ratio of spectrophotometric analysis of the extracted DNA samples was between 1.80-1.92 indicating a highly purified DNA. The gel electrophoresis of amplified PCR products of HBV DNA revealed a single 524 bp band in the test samples. Conclusion: Screening for HBV infection among adolescents by HEPACARD and further confirmation by PCR is recommended to monitor the progression of the disease and antiviral treatment.
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- 2015
8. Study of Bacterial Agents Causing Febrile Illnesses in Children between 1 Month to 12 Years of Age in a Tertiary Care Hospital in Sikkim
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Krishna Agarwal, Jyotsna Kapil, and Pilli Hema Prakash Kumari
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Pediatrics ,medicine.medical_specialty ,business.industry ,medicine ,Tertiary care hospital ,business - Published
- 2020
9. Infections of Biliary Tract
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Pilli, Hema Prakash Kumari and Payala, Vijayalakshmi
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Medical - Abstract
Biliary tract infections include cholangitis and cholecystitis. They are associated with high morbidity and mortality in elderly patients with comorbid disease. The most common infecting organisms are Enterobacteriaceae ascending from the gastrointestinal tract, Gram-positive pathogens like Enterococci spp.; the infections are rarely caused by fungi, viruses, and parasites. The prime reason for biliary tract infections is the ascending infection due to the reflux of duodenal contents and also the blood-borne infection or infection spreading through the portal-venous channels. The other predisposing conditions causing biliary tract infections include critical illnesses such as trauma, burns, sepsis, HIV infection, immunosuppression, diabetes, non-biliary surgery, and childbirth. The infection is reduced by β-lactam antibiotics or their derivatives, cephalosporins, carbapenems, fluoroquinolones, etc. Empiric treatment with piperacillin/tazobactam or a cephalosporin with or without metronidazole is recommended for moderate and severe acute cholecystitis irrespective of whether there is growth by culture. Patients with severe cholecystitis are unfortunately difficult to identify properly, both clinically and radiologically, because clinical symptoms are unexpected, and imaging investigations are frequently ambiguous. However, there are significant differences in morbidity and death rates between individuals with mild cholecystitis and those with severe cholecystitis. Preventing related consequences requires early identification and effective therapy of individuals at risk of severe cholecystitis.
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- 2022
10. Vector-borne and zoonotic infections and their relationships with regional and socioeconomic statuses: An ID-IRI survey in 24 countries of Europe, Africa and Asia
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Nesrin Ghanem-Zoubi, Meltem Taşbakan, Handan Ankarali, Md. Tanvir Rahman, Natalia Pshenichnaya, Taufiquer Rahman, Marta Vecchi, Alma Tokayeva, Maria Pishmisheva-Peleva, Pilli Hema Prakash Kumari, Roxana Carmen Cernat, Mumtaz Ali Khan, Fatma Nurhayat Saydam, Meliha Meric-Koc, Sholpan Kulzhanova, Edmond Puca, Roman Stebel, Kumar Angamuthu, Yasemin Cag, Fahad Almajid, Hakan Erdem, Aleksandra Barac, Nagwa Mostafa El-Sayed, Ravilya Yegemberdiyeva, Zarema Obradović, Gorana Dragovac, Nasim Akhtar, Manar Ezz El-Arab Ramadan, Fatemeh Moradi Mahmuodabad, Ayse Batirel, Antonio Cascio, Dumitru Irina Magdalena, Hakan Evren, Emanuele Nicastri, Marion Le Maréchal, Lenka Fasanekova, Mustafa Dogan, Serhat Uysal, Andrea Marino, Natalia Dirani, Nenad Pandak, Marija Dimzova, Alfonso J. Rodriguez-Morales, Jurica Arapović, Amangul Duisenova, Ilkay Bozkurt, Aamer Ikram, Stanislav Kotsev, Ejaz Ahmed Khan, Rosa Fontana Del Vecchio, Agah Victor Maduka, Ainur Sadykova, Safak Ozer-Balin, Ruxandra Moroti, Rok Čivljak, Amjad Mahboob, Samir Al-Moghazi, Yvon Ruch, MERİÇ KOÇ, MELİHA, Saydam F.N., Erdem H., Ankarali H., El-Arab Ramadan M.E., El-Sayed N.M., Civljak R., Pshenichnaya N., Moroti R.V., Mahmuodabad F.M., Maduka A.V., Mahboob A., Prakash Kumari P.H., Stebel R., Cernat R., Fasanekova L., Uysal S., Tasbakan M., Arapovic J., Magdalena D.I., Angamuthu K., Ghanem-Zoubi N., Meric-Koc M., Ruch Y., Marino A., Sadykova A., Batirel A., Khan E.A., Kulzhanova S., Al-Moghazi S., Yegemberdiyeva R., Nicastri E., Pandak N., Akhtar N., Ozer-Balin S., Cascio A., Dimzova M., Evren H., Puca E., Tokayeva A., Vecchi M., Bozkurt I., Dogan M., Dirani N., Duisenova A., Khan M.A., Kotsev S., Obradovic Z., Del Vecchio R.F., Almajid F., Barac A., Dragovac G., Pishmisheva-Peleva M., Rahman M.T., Rahman T., Le Marechal M., Cag Y., Ikram A., and Rodriguez-Morales A.J.
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Asia ,Surveillance data ,030231 tropical medicine ,education ,Economic statu ,Tick ,Socioeconomic Factor ,Zoonosis ,03 medical and health sciences ,0302 clinical medicine ,Economic status ,Zoonosi ,Multidisciplinary approach ,Zoonoses ,Environmental health ,medicine ,Animals ,Humans ,Hemorrhagic Fever, Crimean ,economic status ,infection ,tick ,vector ,zoonosis ,Socioeconomic status ,health care economics and organizations ,Cross-Sectional Studie ,0303 health sciences ,Zoonotic Infection ,biology ,Animal ,030306 microbiology ,Significant difference ,Public Health, Environmental and Occupational Health ,An ID-IRI survey in 24 countries of Europe, Africa and Asia-, TRAVEL MEDICINE AND INFECTIOUS DISEASE, cilt.44, 2021 [Saydam F. N. , Erdem H., ANKARALI H., Ramadan M. E. E. , El-Sayed N. M. , Civljak R., Pshenichnaya N., Moroti R. V. , Mahmuodabad F. M. , Maduka A. V. , et al., -Vector-borne and zoonotic infections and their relationships with regional and socioeconomic statuses] ,medicine.disease ,biology.organism_classification ,3. Good health ,Europe ,Cross-Sectional Studies ,Infectious Diseases ,Geography ,Socioeconomic Factors ,Vector (epidemiology) ,Africa ,Hemorrhagic Fever Virus, Crimean-Congo ,Vector ,Infection - Abstract
Background: In this cross-sectional, international study, we aimed to analyze vector-borne and zoonotic infections (VBZI), which are significant global threats. Method: VBZIs’ data between May 20–28, 2018 was collected. The 24 Participatingcountries were classified as lower-middle, upper-middle, and high-income. Results: 382 patients were included. 175(45.8%) were hospitalized, most commonly in Croatia, Egypt, and Romania(P = 0.001). There was a significant difference between distributions of VBZIs according to geographical regions(P < 0.001). Amebiasis, Ancylostomiasis, Blastocystosis, Cryptosporidiosis, Giardiasis, Toxoplasmosis were significantly more common in the Middle-East while Bartonellosis, Borreliosis, Cat Scratch Disease, Hantavirus syndrome, Rickettsiosis, Campylobacteriosis, Salmonellosis in Central/East/South-East Europe; Brucellosis and Echinococcosis in Central/West Asia; Campylobacteriosis, Chikungunya, Tick-borne encephalitis, Visceral Leishmaniasis, Salmonellosis, Toxoplasmosis in the North-Mediterranean; CCHF, Cutaneous Leishmaniasis, Dengue, Malaria, Taeniasis, Salmonellosis in Indian Subcontinent; Lassa Fever in West Africa. There were significant regional differences for viral hemorrhagic fevers(P < 0.001) and tick-borne infections(P < 0.001), and according to economic status for VBZIs(P < 0.001). The prevalences of VBZIs were significantly higher in lower-middle income countries(P = 0.001). The most similar regions were the Indian Subcontinent and the Middle-East, the Indian Subcontinent and the North-Mediterranean, and the Middle-East and North-Mediterranean regions. Conclusions: Regional and socioeconomic heterogeneity still exists for VBZIs. Control and eradication of VBZIs require evidence-based surveillance data, and multidisciplinary efforts.
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- 2021
11. Detection of Carbapenem and Colistin Resistant Gram-negative Bacteria in a Tertiary Care Hospital, Visakhapatnam, India
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Ratna Harika Dusi, Alugupalli Amar Sahas, Pilli Hema Prakash Kumari, Payala Vijayalakshmi, Swapna Sasapu, and R. V. Manasa
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Carbapenem ,Gram-negative bacteria ,biology ,business.industry ,Colistin ,Medicine ,General Medicine ,Tertiary care hospital ,biology.organism_classification ,business ,medicine.drug ,Microbiology - Published
- 2018
12. THE COVID-19 VACCINATION ACCEPTANCE/HESITANCY RATE AND ITS DETERMINANTS AMONG HEALTHCARE WORKERS OF 91 COUNTRIES: A MULTICENTER CROSS-SECTIONAL STUDY.
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Askarian, Mehrdad, Semenov, Aleksandr, Llopis, Ferran, Rubulotta, Francesca, Dragovac, Gorana, Pshenichnaya, Natalia, Assadian, Ojan, Ruch, Yvon, Shayan, Zahra, Padilla Fortunatti, Cristobal, Lucey, Daniel, Almohaizeie, Abdullah, Kamal, Abu Hena Mostafa, Ogunshe, Adenike, Konkayev, Aidos, Beg, Asim, Primerano, Enzo, Amer, Fatma, Pilli, Hema Prakash Kumari, and Hung, Ivan
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MEDICAL personnel ,COVID-19 vaccines ,PHYSICIANS ,DETERMINANTS (Mathematics) ,VACCINATION coverage ,VACCINE safety ,VACCINATION ,HUMAN Development Index - Abstract
The aim of this study was to investigate the COVID-19 vaccination acceptance rate and its determinants among healthcare workers in a multicenter study. This was a cross-sectional multi-center survey conducted from February 5 to April 29, 2021 . The questionnaire consisted of 26 items in 6 subscales. The English version of the questionnaire was translated into seven languages and distributed through Google Forms using snowball sampling; a colleague in each country was responsible for the forward and backward translation, and also the distribution of the questionnaire. A forward stepwise logistic regression was utilized to explore the variables and questionnaire factors tied to the intention to COVID-19 vaccination. 4630 participants from 91 countries completed the questionnaire. According to the United Nations Development Program 2020, 43.6 % of participants were from low Human Development Index (HDI) regions, 48.3 % high and very high, and 8.1 % from medium. The overall vaccination hesitancy rate was 37 %. Three out of six factors of the questionnaire were significantly related to intention to the vaccination. While 'Perceived benefits of the COVID-19 vaccination' (OR: 3.82, p-value<0.001) and 'Prosocial norms' (OR: 5.18, p-value<0.001) were associated with vaccination acceptance, 'The vaccine safety/cost concerns' with OR: 3.52, p-value<0.001 was tied to vaccination hesitancy. Medical doctors and pharmacists were more willing to take the vaccine in comparison to others. Importantly, HDI with OR: 12.28, 95 % CI: 6.10-24.72 was a strong positive determinant of COVID-19 vaccination acceptance. This study highlighted the vaccination hesitancy rate of 37 % in our sample among HCWs. Increasing awareness regarding vaccination benefits, confronting the misinformation, and strengthening the prosocial norms would be the primary domains for maximizing the vaccination coverage. The study also showed that the HDI is strongly associated with the vaccination acceptance/ hesitancy, in a way that those living in low HDI contexts are more hesitant to receive the vaccine. [ABSTRACT FROM AUTHOR]
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- 2022
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- View/download PDF
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