10 results on '"Ping-Sheng Leng"'
Search Results
2. Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
- Author
-
Yun-Yao Yang, Bo Ma, Ying-Ying Li, Ming-Zheng Han, Jing Wu, Xiao-Feng Zhou, Ji Tian, Wen-He Wang, Ping-Sheng Leng, and Zeng-Hui Hu
- Subjects
Lilium ‘siberia’ ,flower fragrance ,monoterpenes ,RNA-Seq ,LiMYB305 ,Plant culture ,SB1-1110 - Abstract
Lilium is a popular cut flower that is highly favored by consumers due to its snowy white color and strong fragrance, which originates from the release of monoterpenes. However, the underlying molecular mechanism of monoterpene synthesis remains poorly understood. In this study, the content of three main monoterpenes (linalool, ocimene, and myrcene) was examined in Lilium ‘Siberia’, and RNA sequencing of the 11 stages of flower development was conducted. The biosynthesis of the three monoterpenes increased with flower development, reaching their peak levels at the full flowering stage. Transcriptome data revealed 257,140 unigenes, with an average size of 794 bp, from which 43,934 differentially expressed genes were identified and enriched in the KEGG pathways partly involved in plant hormone signal transduction and monoterpenoid biosynthesis. Furthermore, the essential factor LiMYB305 was identified by WGCNA after the release of the flower fragrance. The transient silencing of LiMYB305 in petals using VIGS technology showed that the mRNA expression levels of LiLiS, LiOcS, and LiMyS were significantly downregulated and that the release of linalool, ocimene, and myrcene had decreased significantly. Y1H, LUC, and EMSA experiments revealed that LiMYB305 directly bound and activated the LiOcS promoter to increase the synthesis of monoterpenes. Taken together, these results provide insight into the molecular mechanism of monoterpene synthesis and provide valuable information to investigate the formation of the flower fragrance in Lilium.
- Published
- 2022
- Full Text
- View/download PDF
3. Ectomycorrhizal Inoculation Enhances the Salt Tolerance of Quercus mongolica Seedlings
- Author
-
Xiao-Ning Bai, Han Hao, Zeng-Hui Hu, and Ping-Sheng Leng
- Subjects
Quercus mongolica ,ectomycorrhiza ,NaCl ,stress ,Botany ,QK1-989 - Abstract
Salt stress harms the growth and development of plants, and the degree of soil salinization in North China is becoming increasingly severe. Ectomycorrhiza (ECM) is a symbiotic system formed by fungi and plants that can improve the growth and salt tolerance of plants. No studies to date have examined the salt tolerance of Quercus mongolica, a typical ectomycorrhizal tree species of temperate forests in the northern hemisphere. Here, we inoculated Q. mongolica with two ectomycorrhizal fungi (Gomphidius viscidus; Suillus luteus) under NaCl stress to characterize the effects of ECM. The results showed that the symbiotic relationship of Q. mongolica with G. viscidus was more stable than that with S. luteus. The cross-sectional area of roots increased after inoculation with the two types of ectomycorrhizal fungi. Compared with the control group, plant height, soluble sugar content, and soluble protein content of leaves were 1.62, 2.41, and 2.04 times higher in the G. viscidus group, respectively. Chlorophyll (Chl) content, stomatal conductance (Gs), and intracellular CO2 concentration (Ci) were significantly higher in Q. mongolica inoculated with ectomycorrhizal fungi than in the control, but differences in the net photosynthetic rate (Pn), transpiration rate (Tr), and photosystem II maximum photochemical efficiency (Fv/Fm) were lower. The relative conductivity of Q. mongolica inoculated with the two ectomycorrhizal fungi was consistently lower than that of non-mycorrhizal seedlings, with the effect of G. viscidus more pronounced than that of S. luteus. The malondialdehyde (MDA) content showed a similar pattern. Peroxidase (POD) and catylase (CAT) levels in mycorrhizal seedlings were generally higher than those of non-mycorrhizal seedlings under normal conditions, and were significantly higher than those of non-mycorrhizal seedlings on the 36th and 48th day after salt treatment, respectively. Overall, the results indicated that the salt tolerance of Q. mongolica seedlings was improved by ectomycorrhizal inoculation.
- Published
- 2021
- Full Text
- View/download PDF
4. LiMYB108 is involved in floral monoterpene biosynthesis induced by light intensity in Lilium ‘Siberia’
- Author
-
Yun-Yao, Yang, Xi, Zhang, Ming-Zheng, Han, Zeng-Hui, Hu, Jing, Wu, Nan, Ma, Ping-Sheng, Leng, and Xiao-Feng, Zhou
- Published
- 2023
- Full Text
- View/download PDF
5. Chromosome‐scale genome assembly and insights into the metabolome and gene regulation of leaf color transition in an important oak species, Quercus dentata
- Author
-
Wen‐Bo Wang, Xiang‐Feng He, Xue‐Mei Yan, Bo Ma, Cun‐Fu Lu, Jing Wu, Yi Zheng, Wen‐He Wang, Wen‐Bo Xue, Xue-Chan Tian, Jing-Fang Guo, Yousry A. El‐Kassaby, Ilga Porth, Ping‐Sheng Leng, Zeng‐Hui Hu, and Jian‐Feng Mao
- Subjects
co-expression network ,Quercus ,Genetics and Breeding in Agricultural Sciences ,Physiology ,flavonoids ,leaf color transition ,Plant Science ,gene regulation ,Genetik och förädling inom lantbruksvetenskap - Abstract
Quercus dentata Thunb., a dominant forest tree species in northern China, has significant ecological and ornamental value due to its adaptability and beautiful autumn coloration, with color changes from green to yellow into red resulting from the autumnal shifts in leaf pigmentation. However, the key genes and molecular regulatory mechanisms for leaf color transition remain to be investigated. First, we presented a high-quality chromosome-scale assembly for Q. dentata. This 893.54 Mb sized genome (contig N50 = 4.21 Mb, scaffold N50 = 75.55 Mb; 2n = 24) harbors 31 584 protein-coding genes. Second, our metabolome analyses uncovered pelargonidin-3-O-glucoside, cyanidin-3-O-arabinoside, and cyanidin-3-O-glucoside as the main pigments involved in leaf color transition. Third, gene co-expression further identified the MYB-bHLH-WD40 (MBW) transcription activation complex as central to anthocyanin biosynthesis regulation. Notably, transcription factor (TF) QdNAC (QD08G038820) was highly co-expressed with this MBW complex and may regulate anthocyanin accumulation and chlorophyll degradation during leaf senescence through direct interaction with another TF, QdMYB (QD01G020890), as revealed by our further protein–protein and DNA–protein interaction assays. Our high-quality genome assembly, metabolome, and transcriptome resources further enrich Quercus genomics and will facilitate upcoming exploration of ornamental values and environmental adaptability in this important genus.
- Published
- 2023
6. [Ectomycorrhizal fungi enhance salt tolerance of
- Author
-
Wei, Guo, Han, Hao, Wei-Hao, Zhang, Zeng-Hui, Hu, and Ping-Sheng, Leng
- Abstract
We explored the effects of salt stress on the growth of为探究盐胁迫对蒙古栎生长的影响以及外生菌根真菌(ECMF)对蒙古栎离子平衡的调节作用,对蒙古栎幼苗接种4种ECMF(铆钉菇、褐环乳牛肝菌、厚环粘盖牛肝菌和美味牛肝菌)后,以1年生非菌根化与菌根化幼苗为试验材料,进行36 d的NaCl胁迫(0、100、200、300 mmol·L
- Published
- 2023
7. Lilac (Syringa oblata) genome provides insights into its evolution and molecular mechanism of petal color change
- Author
-
Bo Ma, Jing Wu, Tian-Le Shi, Yun-Yao Yang, Wen-Bo Wang, Yi Zheng, Shu-Chai Su, Yun-Cong Yao, Wen-Bo Xue, Ilga Porth, Yousry A. El-Kassaby, Ping-Sheng Leng, Zeng-Hui Hu, and Jian-Feng Mao
- Subjects
Light ,Pigmentation ,Metabolome ,Medicine (miscellaneous) ,Flowers ,Syringa ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Color change during flower opening is common; however, little is understood on the biochemical and molecular basis related. Lilac (Syringa oblata), a well-known woody ornamental plant with obvious petal color changes, is an ideal model. Here, we presented chromosome-scale genome assembly for lilac, resolved the flavonoids metabolism, and identified key genes and potential regulatory networks related to petal color change. The genome assembly is 1.05 Gb anchored onto 23 chromosomes, with a BUSCO score of 96.6%. Whole-genome duplication (WGD) event shared within Oleaceae was revealed. Metabolome quantification identified delphinidin-3-O-rutinoside (Dp3Ru) and cyanidin-3-O-rutinoside (Cy3Ru) as the major pigments; gene co-expression networks indicated WRKY an essential regulation factor at the early flowering stage, ERF more important in the color transition period (from violet to light nearly white), while the MBW complex participated in the entire process. Our results provide a foundation for functional study and molecular breeding in lilac.
- Published
- 2021
8. Ectomycorrhizal Inoculation Enhances the Salt Tolerance of Quercus mongolica Seedlings
- Author
-
Zeng-Hui Hu, Han Hao, Ping-Sheng Leng, and Xiao-Ning Bai
- Subjects
Stomatal conductance ,Ecology ,biology ,Inoculation ,Communication ,fungi ,Gomphidius ,Botany ,Suillus luteus ,Plant Science ,biology.organism_classification ,Photosynthesis ,ectomycorrhiza ,Quercus mongolica ,Ectomycorrhiza ,stress ,chemistry.chemical_compound ,Horticulture ,NaCl ,chemistry ,QK1-989 ,Chlorophyll ,Ecology, Evolution, Behavior and Systematics ,Transpiration - Abstract
Salt stress harms the growth and development of plants, and the degree of soil salinization in North China is becoming increasingly severe. Ectomycorrhiza (ECM) is a symbiotic system formed by fungi and plants that can improve the growth and salt tolerance of plants. No studies to date have examined the salt tolerance of Quercus mongolica, a typical ectomycorrhizal tree species of temperate forests in the northern hemisphere. Here, we inoculated Q. mongolica with two ectomycorrhizal fungi (Gomphidius viscidus; Suillus luteus) under NaCl stress to characterize the effects of ECM. The results showed that the symbiotic relationship of Q. mongolica with G. viscidus was more stable than that with S. luteus. The cross-sectional area of roots increased after inoculation with the two types of ectomycorrhizal fungi. Compared with the control group, plant height, soluble sugar content, and soluble protein content of leaves were 1.62, 2.41, and 2.04 times higher in the G. viscidus group, respectively. Chlorophyll (Chl) content, stomatal conductance (Gs), and intracellular CO2 concentration (Ci) were significantly higher in Q. mongolica inoculated with ectomycorrhizal fungi than in the control, but differences in the net photosynthetic rate (Pn), transpiration rate (Tr), and photosystem II maximum photochemical efficiency (Fv/Fm) were lower. The relative conductivity of Q. mongolica inoculated with the two ectomycorrhizal fungi was consistently lower than that of non-mycorrhizal seedlings, with the effect of G. viscidus more pronounced than that of S. luteus. The malondialdehyde (MDA) content showed a similar pattern. Peroxidase (POD) and catylase (CAT) levels in mycorrhizal seedlings were generally higher than those of non-mycorrhizal seedlings under normal conditions, and were significantly higher than those of non-mycorrhizal seedlings on the 36th and 48th day after salt treatment, respectively. Overall, the results indicated that the salt tolerance of Q. mongolica seedlings was improved by ectomycorrhizal inoculation.
- Published
- 2021
9. [Influences of municipal sludge applied in slope vegetation restoration on surface water environment]
- Author
-
Chen Guang, Zhen, Ping Sheng, Leng, Li Juan, Liu, De Quan, Dou, and Zeng Hui, Hu
- Subjects
Soil ,Coal ,Sewage ,Nitrogen ,Metals, Heavy ,Plant Development ,Soil Pollutants ,Phosphorus ,Plants ,Polycyclic Aromatic Hydrocarbons - Abstract
The application of municipal sludge in ecological restoration has a good prospect for avoiding the food chain of grain crops, but its influences on surface water environmental are unclear. The municipal sludge and construction waste were mixed with 1:1 (V/V) as growth media, which were covered over simulation coal gangue slopes. Eight native woody species were sowed in the mixed media. The plant growth and coverage, as well as conductivity, pH, the concentrations of nitrogen (N), phosphorus (P), potassium (K), heavy metal and polycyclic aromatic hydrocarbon (PAHs) of surface and underground runoff of the slopes in the growing season were investigated. The results showed that plants grew well on the mixed media. The average plant coverage reached 60%. The pH of the surface and underground runoff changed little and near to neutral. The conductivity, N, P, K, heavy metal and PAHs contents of the slope runoff were high. The N and P contents in the growing season were above the National Standards of Surface Water Quality (GB 3838-2002) V. The contents of heavy metal were the highest in July. The contents of As lied at the GB IV-V, whereas other heavy metal contents up to GB II-IV. With strong rain leaching in the summer as well as the absorption, degrading and fix effect of plant-soil system on chemical substrates, the conductivity and N, P, K, heavy metal and PAHs contents of the slope runoff significantly decreased. The contents of heavy metal in late stage of growing season arrived at GB 2-3. The contents of PAHs reduced by about 50%. The direct application of municipal sludge in ecological restoration of coal gangue slope were beneficial to plant growth. The plant-soil system might gradually decrease the harmful substance concentrations in the growth media. The negative influences on surface water environment mainly came from eutrophication of N and P. Generally, the environmental safety is manageable.将城市污泥应用于生态恢复因能避开粮食作物食物链而具有良好前景,但其对地表水环境影响仍不十分清楚.本文将城市污泥与建筑垃圾按1∶1体积比混合为生长基质,覆盖在模拟的煤矸石边坡上,播种8种乡土木本植物,对生长季植物生长情况以及坡面地表水和下渗水的电导率、pH、氮磷钾、重金属、多环芳烃等含量进行分析.结果表明: 坡面植物生长良好,平均覆盖度达60%;地表水和下渗水的pH值近中性,变化不大,而电导率、氮磷钾、重金属和多环芳烃含量均较高,其中,氮、磷含量在整个生长季超过国家地表水环境质量Ⅴ类水质标准;7月重金属含量最高,其中,As含量只达地表水环境质量Ⅳ~Ⅴ类标准,其余重金属含量多达Ⅱ~Ⅳ标准;随着夏季雨水淋洗增加以及植物-土壤系统对化学物质的吸收、降解和固着作用,地表水和下渗水的电导率、氮磷钾、重金属和多环芳烃含量均显著下降,生长季后期重金属含量达到地表水环境质量Ⅱ~Ⅲ类标准,多环芳烃含量减少约一半.将城市污泥直接应用在煤矸石边坡生态恢复中有利于植物生长,植物-土壤系统使得生长基质中的有害物含量逐渐降低,对地表水环境的负影响主要表现为氮、磷的富营养化,但总体上其环境安全性可控.
- Published
- 2018
10. Simultaneous Determination of L-Ascorbic Acid and L-Ascorbylpalmitate Using RP-HPLC Method
- Author
-
Ye Lin Tian, Ping Sheng Leng, Yun Liu, and Jun Kai Wang
- Subjects
Detection limit ,Chromatography ,Methyl palmitate ,Chemistry ,General Engineering ,Transesterification ,Uv detection ,Ascorbic acid - Abstract
A rapid reversed phase highperformance liquid chromatography (RP-HPLC) method was developed for the simultaneous analysis of L-ascorbic acid and L-ascorbylpalmitate (AP). The chromatography was performed on a SSI model 2300-525 high performance liquid chromatographequipped with anAlltechApollo C18 column at 30 oC.The mobile phase was acetonitrile-water (90:10,v/v) with the flow rate of 1.0 mL/min. UV detection wavelength was 250 nm. This method permits the simultaneous determination of ascorbic acid and AP in the synthesis of AP transesterified with methyl palmitate and ascorbic acid. The detection limit of ascorbic acid and AP was 0.07μg/mL and 0.12μg/mL, respectively. The recovery was 90.59 ± 3.04% for ascorbic acid and 101.3 ± 4.81% for AP. The linearity range for ascorbic acid and AP was in the range of 0.1 - 0.7 mg/mL and 0.4 - 4.0 mg/mL, respectively. Correlation coefficients (R2) were 0.9910 for ascorbic acid and 0.9986 for AP. The proposed method could be used for routine quality control of AP synthesis with methyl palmitate and ascorbic acidby enzymatic transesterification.
- Published
- 2013
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.