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1. Combination drug screen targeting glioblastoma core vulnerabilities reveals pharmacological synergisms

Author

Ariey-Bonnet, Jérémy, Berges, Raphael, Montero, Marie-Pierre, Mouysset, Baptiste, Piris, Patricia, Muller, Kevin, Pinna, Guillaume, Failes, Tim W., Arndt, Greg M., Morando, Philippe, Baeza-Kallee, Nathalie, Colin, Carole, Chinot, Olivier, Braguer, Diane, Morelli, Xavier, André, Nicolas, Carré, Manon, Tabouret, Emeline, Figarella-Branger, Dominique, Le Grand, Marion, and Pasquier, Eddy

Published
2023
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3. Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1

Author

Bakail, May, Gaubert, Albane, Andreani, Jessica, Moal, Gwenaëlle, Pinna, Guillaume, Boyarchuk, Ekaterina, Gaillard, Marie-Cécile, Courbeyrette, Regis, Mann, Carl, Thuret, Jean-Yves, Guichard, Bérengère, Murciano, Brice, Richet, Nicolas, Poitou, Adeline, Frederic, Claire, Le Du, Marie-Hélène, Agez, Morgane, Roelants, Caroline, Gurard-Levin, Zachary A., Almouzni, Geneviève, Cherradi, Nadia, Guerois, Raphael, and Ochsenbein, Françoise

Published
2019
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5. Tumor-Targeted Perfluorinated Micelles as Efficient Theranostic Agents Combining Positron Emission Tomography and Radiosensitization

Author

Godel-Pastre, Sophia, Porcel, Erika, Pinna, Guillaume, Vandamme, Marie, Denis, Caroline, Leterrier, Claire, Doris, Eric, Truillet, Charles, and Gravel, Edmond

Abstract

We report the synthesis of biocompatible perfluorinated micelles designed to improve radiotherapeutic efficacy in a radioresistant tumor environment. In vitro and in vivo behaviors of perfluorinated micelles were assessed at both cellular and tissular levels. The micellar platform offers key advantages as theranostic tool: (i) small size, allowing deep tissue penetration; (ii) oxygen transport to hypoxic tissues; (iii) negligible toxicity in the absence of ionizing radiation; (iv) internalization into cancer cells; (v) potent radiosensitizing effect; and (vi) excellent tumor-targeting properties, as monitored by positron emission tomography. We have demonstrated strong in vitro radiosensitizing effects of the micelle and in vivo tumor targeting, making this nanometric carrier a promising tool for the potentiation of focused radiotherapy.

Published
2024
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7. Supplementary Table 5 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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8. Supplementary Table 3 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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9. Supplementary Table 4 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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10. Supplementary Table 7 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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11. Supplementary Methods from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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12. Supplementary Table 6 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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13. Supplementary Figures 1 - 14 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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14. Supplementary Figure Legends from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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15. Supplementary Methods and Materials from A Novel Epidermal Growth Factor Receptor Inhibitor Promotes Apoptosis in Non–Small Cell Lung Cancer Cells Resistant to Erlotinib

Author

de La Motte Rouge, Thibault, primary, Galluzzi, Lorenzo, primary, Olaussen, Ken A., primary, Zermati, Yael, primary, Tasdemir, Ezgi, primary, Robert, Thomas, primary, Ripoche, Hugues, primary, Lazar, Vladimir, primary, Dessen, Philippe, primary, Harper, Francis, primary, Pierron, Gerard, primary, Pinna, Guillaume, primary, Araujo, Natalia, primary, Harel-Belan, Annick, primary, Armand, Jean-Pierre, primary, Wong, Tai Wai, primary, Soria, Jean Charles, primary, and Kroemer, Guido, primary

Published
2023
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16. Supplementary Table 2 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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17. Supplementary Table 1 from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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18. Data from ALDH1-Positive Cancer Stem Cells Predict Engraftment of Primary Breast Tumors and Are Governed by a Common Stem Cell Program

Author

Charafe-Jauffret, Emmanuelle, primary, Ginestier, Christophe, primary, Bertucci, François, primary, Cabaud, Olivier, primary, Wicinski, Julien, primary, Finetti, Pascal, primary, Josselin, Emmanuelle, primary, Adelaide, José, primary, Nguyen, Tien-Tuan, primary, Monville, Florence, primary, Jacquemier, Jocelyne, primary, Thomassin-Piana, Jeanne, primary, Pinna, Guillaume, primary, Jalaguier, Aurélie, primary, Lambaudie, Eric, primary, Houvenaeghel, Gilles, primary, Xerri, Luc, primary, Harel-Bellan, Annick, primary, Chaffanet, Max, primary, Viens, Patrice, primary, and Birnbaum, Daniel, primary

Published
2023
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19. Data from A Novel Epidermal Growth Factor Receptor Inhibitor Promotes Apoptosis in Non–Small Cell Lung Cancer Cells Resistant to Erlotinib

Author

de La Motte Rouge, Thibault, primary, Galluzzi, Lorenzo, primary, Olaussen, Ken A., primary, Zermati, Yael, primary, Tasdemir, Ezgi, primary, Robert, Thomas, primary, Ripoche, Hugues, primary, Lazar, Vladimir, primary, Dessen, Philippe, primary, Harper, Francis, primary, Pierron, Gerard, primary, Pinna, Guillaume, primary, Araujo, Natalia, primary, Harel-Belan, Annick, primary, Armand, Jean-Pierre, primary, Wong, Tai Wai, primary, Soria, Jean Charles, primary, and Kroemer, Guido, primary

Published
2023
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20. Supplementary Table 1 from A Novel Epidermal Growth Factor Receptor Inhibitor Promotes Apoptosis in Non–Small Cell Lung Cancer Cells Resistant to Erlotinib

Author

de La Motte Rouge, Thibault, primary, Galluzzi, Lorenzo, primary, Olaussen, Ken A., primary, Zermati, Yael, primary, Tasdemir, Ezgi, primary, Robert, Thomas, primary, Ripoche, Hugues, primary, Lazar, Vladimir, primary, Dessen, Philippe, primary, Harper, Francis, primary, Pierron, Gerard, primary, Pinna, Guillaume, primary, Araujo, Natalia, primary, Harel-Belan, Annick, primary, Armand, Jean-Pierre, primary, Wong, Tai Wai, primary, Soria, Jean Charles, primary, and Kroemer, Guido, primary

Published
2023
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21. A genome‐wide RNAi screen reveals essential therapeutic targets of breast cancer stem cells

Author

Arfaoui, Abir, Rioualen, Claire, Azzoni, Violette, Pinna, Guillaume, Finetti, Pascal, Wicinski, Julien, Josselin, Emmanuelle, Macario, Manon, Castellano, Rémy, Léonard‐Stumpf, Candi, Bal, Anthony, Gros, Abigaelle, Lossy, Sylvain, Kharrat, Maher, Collette, Yves, Bertucci, Francois, Birnbaum, Daniel, Douik, Hayet, Bidaut, Ghislain, Charafe‐Jauffret, Emmanuelle, and Ginestier, Christophe

Published
2019
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22. OGG1 competitive inhibitors show important off-target effects by directly inhibiting efflux pumps and disturbing mitotic progression

Author

Tanushi, Xhaferr, primary, Pinna, Guillaume, additional, Vandamme, Marie, additional, Siberchicot, Capucine, additional, D’Augustin, Ostiane, additional, Di Guilmi, Anne-Marie, additional, Radicella, J. Pablo, additional, Castaing, Bertrand, additional, Smith, Rebecca, additional, Huet, Sebastien, additional, Leteurtre, François, additional, and Campalans, Anna, additional

Published
2023
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23. Combination drug screen targeting glioblastoma core vulnerabilities reveals pharmacological synergisms

Author

Ariey-Bonnet, Jérémy, primary, Berges, Raphael, additional, Montero, Marie-Pierre, additional, Mouysset, Baptiste, additional, Piris, Patricia, additional, Muller, Kevin, additional, Pinna, Guillaume, additional, Failes, Tim W., additional, Arndt, Greg M., additional, Baeza-Kallee, Nathalie, additional, Colin, Carole, additional, Chinot, Olivier, additional, Braguer, Diane, additional, Morelli, Xavier, additional, André, Nicolas, additional, Carré, Manon, additional, Tabouret, Emeline, additional, Figarella-Branger, Dominique, additional, Grand, Marion Le, additional, and Pasquier, Eddy, additional

Published
2022
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24. Small Regulatory RNAs and Skeletal Muscle Cell Differentiation

Author

Polesskaya, Anna, Naguibneva, Irina, Ameyar-Zazoua, Maya, Degerny, Cindy, Kropp, Jeremie, Nonne, Nora, Mercatelli, Neri, Souidi, Mouloud, Kratassiouk, Gueorgui, Pinna, Guillaume, Pritchard, Linda L., Harel-Bellan, Annick, Capasso, Vincenzo, editor, Gromov, Misha, editor, Harel-Bellan, Annick, editor, Morozova, Nadya, editor, and Pritchard, Linda Louise, editor

Published
2013
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27. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity

Author

Mbianda, Johanne, primary, Bakail, May, additional, André, Christophe, additional, Moal, Gwenaëlle, additional, Perrin, Marie E., additional, Pinna, Guillaume, additional, Guerois, Raphaël, additional, Becher, Francois, additional, Legrand, Pierre, additional, Traoré, Seydou, additional, Douat, Céline, additional, Guichard, Gilles, additional, and Ochsenbein, Françoise, additional

Published
2021
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28. Chromatin recruitment of OGG1 requires cohesin and mediator and is essential for efficient 8-oxoG removal

Author

Lebraud, Emilie, Pinna, Guillaume, Siberchicot, Capucine, Depagne, Jordane, Busso, Didier, Fantini, Damiano, Irbah, Lamya, Robeska, Elena, Kratassiouk, Gueorgui, Ravanat, Jean-Luc, Epe, Bernd, Radicella, J Pablo, Campalans, Anna, Stabilité génétique, Cellules Souches et Radiations (SCSR (U_967)), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), PARi (PARI), Département Plateforme (PF I2BC), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Plateforme de protéomique, Institut de radiobiologie cellulaire et moléculaire (iRCM), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Radiobiologie Cellulaire et Moléculaire (IRCM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Département Interfaces pour l'énergie, la Santé et l'Environnement (DIESE), Institute of Pharmacy and Biochemistry [Mainz, Germany], Johannes Gutenberg - Universität Mainz (JGU), Laboratoire de Recherche sur l'Instabilité Génétique (LRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), ANR-18-CE92-0013,TG-TOX,Mécanismes de réparation et toxicité des glycoles de thymine, une des principales lésions oxydatives de l'ADN(2018), and Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)

Subjects
Guanine, DNA Repair, AcademicSubjects/SCI00010, Chromosomal Proteins, Non-Histone, Cell Cycle Proteins, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Genome Integrity, Repair and Replication, Transfection, Chromatin, Genomic Instability, DNA Glycosylases, Euchromatin, Oxidative Stress, Humans, RNA, Small Interfering, ComputingMilieux_MISCELLANEOUS, HeLa Cells
Abstract

One of the most abundant DNA lesions induced by oxidative stress is the highly mutagenic 8-oxoguanine (8-oxoG), which is specifically recognized by 8-oxoguanine DNA glycosylase 1 (OGG1) to initiate its repair. How DNA glycosylases find small non-helix-distorting DNA lesions amongst millions of bases packaged in the chromatin-based architecture of the genome remains an open question. Here, we used a high-throughput siRNA screening to identify factors involved in the recognition of 8-oxoG by OGG1. We show that cohesin and mediator subunits are required for re-localization of OGG1 and other base excision repair factors to chromatin upon oxidative stress. The association of OGG1 with euchromatin is necessary for the removal of 8-oxoG. Mediator subunits CDK8 and MED12 bind to chromatin and interact with OGG1 in response to oxidative stress, suggesting they participate in the recruitment of the DNA glycosylase. The oxidative stress-induced association between the cohesin and mediator complexes and OGG1 reveals an unsuspected function of those complexes in the maintenance of genomic stability.

Published
2020

29. Additional file 1 of MasterPATH: network analysis of functional genomics screening data

Author

Rubanova, Natalia, Pinna, Guillaume, Kropp, Jeremie, Campalans, Anna, Radicella, Juan Pablo, Polesskaya, Anna, Harel-Bellan, Annick, and Morozova, Nadya

Abstract

Additional file 1: Supplementary note 1. Table S1. Hit genes for miRNA loss-of-function screen of human muscle differentiation process. Table S2. Hit genes for transcriptome profiling of human muscle differentiation process. Table S3. Hit genes for human oxidative DNA damage recognition loss-of-function screen. Table S4. Topological features of the integrated and PPI networks.

Published
2020
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31. Small Regulatory RNAs and Skeletal Muscle Cell Differentiation

Author

Polesskaya, Anna, primary, Naguibneva, Irina, additional, Ameyar-Zazoua, Maya, additional, Degerny, Cindy, additional, Kropp, Jeremie, additional, Nonne, Nora, additional, Mercatelli, Neri, additional, Souidi, Mouloud, additional, Kratassiouk, Gueorgui, additional, Pinna, Guillaume, additional, Pritchard, Linda L., additional, and Harel-Bellan, Annick, additional

Published
2012
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32. CPEB1 orchestrates a fine-tuning of miR-145-5p tumor-suppressive activity on TWIST1 translation in prostate cancer cells

Author

Rajabi, Fatemeh, primary, Liu-Bordes, Win-Yan, additional, Pinskaya, Marina, additional, Dominika, Foretek, additional, Kratassiouk, Gueorgui, additional, Pinna, Guillaume, additional, Nanni, Simona, additional, Farsetti, Antonella, additional, Gespach, Christian, additional, Londoño-Vallejo, Arturo, additional, and Groisman, Irina, additional

Published
2020
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35. Conception sur une base rationnelle de peptides de haute affinité inhibant l'histone chaperon ASF1

Author

Bakail, May, Gaubert, Albane, Andreani, Jessica, Moal, Gwenaëlle, Pinna, Guillaume, Boyarchuk, Ekaterina, Gaillard, Marie-Cécile, Courbeyrette, Régis, Mann, Carl, Thuret, Jean-Yves, Guichard, Berengère, Murciano, Brice, Richet, Nicolas, Poitou, Adeline, Frederic, Claire, Le Du, Marie-Hélène, Agez, Morgane, Roelants, Caroline, Gurard-Levin, Zachary, Almouzni, Geneviève, Cherradi, Nadia, Guérois, Raphaël, Ochsenbein, Françoise, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Assemblage moléculaire et intégrité du génome (AMIG), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Structurale et Radiobiologie (LBSR), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), PARi (PARI), Département Plateforme (PF I2BC), Sorbonne Université - Faculté de Médecine (SU FM), Sorbonne Université (SU), Sénescence et stabilité génomique (SEN), Département Biologie des Génomes (DBG), Vaccination Antiparasitaire : Laboratoire de Biologie Cellulaire et Moléculaire (LBCM), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Enveloppe Nucléaire, Télomères et Réparation de l’ADN (INTGEN), Institut de Biologie et de Technologies de Saclay (IBITECS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Invasion mechanisms in angiogenesis and cancer (IMAC), Biologie du Cancer et de l'Infection (BCI ), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut National de la Santé et de la Recherche Médicale (INSERM), Dynamique du noyau [Institut Curie], and Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Rosetta Design, Drug Design, [SDV]Life Sciences [q-bio], X-Ray Crystallography, Peptide Inhibitor, Epigenetics, MESH: Amino Acid Sequence, Binding Sites, Histones / metabolism, Neoplasms / drug therapy, Transplantation, Homologous, Cell Penetrating Peptide, Chromatin, Protein-Protein Interaction, Protein Binding, Cancer, [SHS]Humanities and Social Sciences
Abstract

International audience; Anti-silencing function 1 (ASF1) is a conserved H3-H4 histone chaperone involved in histone dynamics during replication, transcription, and DNA repair. Overexpressed in proliferating tissues including many tumors, ASF1 has emerged as a promising therapeutic target. Here, we combine structural, computational, and biochemical approaches to design peptides that inhibit the ASF1-histone interaction. Starting from the structure of the human ASF1-histone complex, we developed a rational design strategy combining epitope tethering and optimization of interface contacts to identify a potent peptide inhibitor with a dissociation constant of 3 nM. When introduced into cultured cells, the inhibitors impair cell proliferation, perturb cell-cycle progression, and reduce cell migration and invasion in a manner commensurate with their affinity for ASF1. Finally, we find that direct injection of the most potent ASF1 peptide inhibitor in mouse allografts reduces tumor growth. Our results open new avenues to use ASF1 inhibitors as promising leads for cancer therapy.; La fonction anti-silencing 1 (ASF1) est un chaperon d'histone H3-H4 conservé, impliqué dans la dynamique des histones pendant la réplication, la transcription et la réparation de l'ADN. Surexprimée dans les tissus en prolifération, y compris dans de nombreuses tumeurs, l'ASF1 est devenue une cible thérapeutique prometteuse. Ici, nous combinons des approches structurelles, informatiques et biochimiques pour concevoir des peptides qui inhibent l'interaction ASF1-histone. En partant de la structure du complexe ASF1-histone humain, nous avons mis au point une stratégie de conception rationnelle combinant la fixation des épitopes et l'optimisation des contacts d'interface pour identifier un puissant inhibiteur peptidique avec une constante de dissociation de 3 nM. Lorsqu'ils sont introduits dans des cellules en culture, les inhibiteurs entravent la prolifération cellulaire, perturbent la progression du cycle cellulaire et réduisent la migration et l'invasion des cellules d'une manière proportionnelle à leur affinité pour l'ASF1. Enfin, nous constatons que l'injection directe du plus puissant inhibiteur du peptide ASF1 dans les allogreffes de souris réduit la croissance des tumeurs. Nos résultats ouvrent de nouvelles voies pour utiliser les inhibiteurs de l'ASF1 comme des pistes prometteuses pour le traitement du cancer.

Published
2019

36. Ouabain and chloroquine trigger senolysis of BRAF‐V600E‐induced senescent cells by targeting autophagy.

Author

L'Hôte, Valentin, Courbeyrette, Régis, Pinna, Guillaume, Cintrat, Jean‐Christophe, Le Pavec, Gwenaëlle, Delaunay‐Moisan, Agnès, Mann, Carl, and Thuret, Jean‐Yves

Subjects
OUABAIN, CHLOROQUINE, AUTOPHAGY, ION transport (Biology), CELLULAR aging, CELL death
Abstract

The expression of BRAF‐V600E triggers oncogene‐induced senescence in normal cells and is implicated in the development of several cancers including melanoma. Here, we report that cardioglycosides such as ouabain are potent senolytics in BRAF senescence. Sensitization by ATP1A1 knockdown and protection by supplemental potassium showed that senolysis by ouabain was mediated by the Na,K‐ATPase pump. Both ion transport inhibition and signal transduction result from cardioglycosides binding to Na,K‐ATPase. An inhibitor of the pump that does not trigger signaling was not senolytic despite blocking ion transport, demonstrating that signal transduction is required for senolysis. Ouabain triggered the activation of Src, p38, Akt, and Erk in BRAF‐senescent cells, and signaling inhibitors prevented cell death. The expression of BRAF‐V600E increased ER stress and autophagy in BRAF‐senescent cells and sensitized the cell to senolysis by ouabain. Ouabain inhibited autophagy flux, which was restored by signaling inhibitors. Consequently, we identified autophagy inhibitor chloroquine as a novel senolytic in BRAF senescence based on the mode of action of cardioglycosides. Our work underlies the interest of characterizing the mechanisms of senolytics to discover novel compounds and identifies the endoplasmic reticulum stress‐autophagy tandem as a new vulnerability in BRAF senescence that can be exploited for the development of further senolytic strategies. [ABSTRACT FROM AUTHOR]

Published
2021
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40. A genome‐wide RNA i screen reveals essential therapeutic targets of breast cancer stem cells

Author

Arfaoui, Abir, primary, Rioualen, Claire, additional, Azzoni, Violette, additional, Pinna, Guillaume, additional, Finetti, Pascal, additional, Wicinski, Julien, additional, Josselin, Emmanuelle, additional, Macario, Manon, additional, Castellano, Rémy, additional, Léonard‐Stumpf, Candi, additional, Bal, Anthony, additional, Gros, Abigaelle, additional, Lossy, Sylvain, additional, Kharrat, Maher, additional, Collette, Yves, additional, Bertucci, Francois, additional, Birnbaum, Daniel, additional, Douik, Hayet, additional, Bidaut, Ghislain, additional, Charafe‐Jauffret, Emmanuelle, additional, and Ginestier, Christophe, additional

Published
2019
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42. Glucocorticoids delay RAF-induced senescence promoted by EGR1

Author

Carvalho, Cyril, primary, L'Hôte, Valentin, additional, Courbeyrette, Régis, additional, Kratassiouk, Gueorgui, additional, Pinna, Guillaume, additional, Cintrat, Jean-Christophe, additional, Denby-Wilkes, Cyril, additional, Derbois, Céline, additional, Olaso, Robert, additional, Deleuze, Jean-François, additional, Mann, Carl, additional, and Thuret, Jean-Yves, additional

Published
2019
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47. miR-600 Acts as a Bimodal Switch that Regulates Breast Cancer Stem Cell Fate through WNT Signaling

Author

El Helou, Rita, primary, Pinna, Guillaume, additional, Cabaud, Olivier, additional, Wicinski, Julien, additional, Bhajun, Ricky, additional, Guyon, Laurent, additional, Rioualen, Claire, additional, Finetti, Pascal, additional, Gros, Abigaelle, additional, Mari, Bernard, additional, Barbry, Pascal, additional, Bertucci, Francois, additional, Bidaut, Ghislain, additional, Harel-Bellan, Annick, additional, Birnbaum, Daniel, additional, Charafe-Jauffret, Emmanuelle, additional, and Ginestier, Christophe, additional

Published
2017
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