24 results on '"Pique, Claudine"'
Search Results
2. HBZ upregulates myoferlin expression to facilitate HTLV-1 infection.
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Polakowski, Nicholas, Sarker, Md Abu Kawsar, Hoang, Kimson, Boateng, Georgina, Rushing, Amanda W., Kendle, Wesley, Pique, Claudine, Green, Patrick L., Panfil, Amanda R., and Lemasson, Isabelle
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LYSOSOMES , *HTLV-I , *GENE expression , *PLANT viruses - Abstract
The complex retrovirus, human T-cell leukemia virus type 1 (HTLV-1), primarily infects CD4+ T-cells in vivo. Infectious spread within this cell population requires direct contact between virally-infected and target cells. The HTLV-1 accessory protein, HBZ, was recently shown to enhance HTLV-1 infection by activating intracellular adhesion molecule 1 (ICAM-1) expression, which promotes binding of infected cells to target cells and facilitates formation of a virological synapse. In this study we show that HBZ additionally enhances HTLV-1 infection by activating expression of myoferlin (MyoF), which functions in membrane fusion and repair and vesicle transport. Results from ChIP assays and quantitative reverse transcriptase PCR indicate that HBZ forms a complex with c-Jun or JunB at two enhancer sites within the MYOF gene and activates transcription through recruitment of the coactivator p300/CBP. In HTLV-1-infected T-cells, specific inhibition of MyoF using the drug, WJ460, or shRNA-mediated knockdown of MyoF reduced infection efficiency. This effect was associated with a decrease in cell adhesion and an intracellular reduction in the abundance of HTLV-1 envelope (Env) surface unit (SU) and transmembrane domain (TM). Lysosomal protease inhibitors partially restored SU levels in WJ460-treated cells, and SU localization to LAMP-2 sites was increased by MyoF knockdown, suggesting that MyoF restricts SU trafficking to lysosomes for degradation. Consistent with these effects, less SU was associated with cell-free virus particles. Together, these data suggest that MyoF contributes to HTLV-1 infection through modulation of Env trafficking and cell adhesion. Author summary: Human T-cell leukemia virus type 1 (HTLV-1) infection can cause a progressive neuroinflammatory disease and a fatal form of leukemia and is also linked to other inflammatory-based pathologies. These clinical manifestations stem, in part, from viral spread within the CD4+ T-cell population of the host. Once infected with HTLV-1, a T-cell adopts a novel gene expression program that renders it competent for infecting other T-cells. This reprogramming event relies on the functions of specific HTLV-1 proteins. Here, we show that induction of myoferlin (MyoF) expression by the viral protein HBZ is important for HTLV-1 infection. Our data support a model in which HBZ activates transcription of the MYOF gene by associating with c-Jun or JunB at two sites within the gene and recruiting the cellular coactivator p300/CBP to these sites. We found that, in connection with its known role in vesicle trafficking, MyoF reduces transfer of the HTLV-1 envelope (Env) protein to lysosomes for degradation, leading to more Env incorporation into virions. Additionally, we found that MyoF enhances the adhesion properties of the HTLV-1-infected T-cells, which is important, as cell-to-cell contact is essential for viral infection. Our study provides new insights into the events required for HTLV-1 infection. [ABSTRACT FROM AUTHOR]
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- 2023
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3. New insights into HTLV entry.
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Pique, Claudine, Hermine, Olivier, Ruscetti, Francis W., and Jones, Kathryn S.
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HTLV , *MOLECULAR biology - Abstract
An abstract of the research paper titled," New insights into the Human T-cell leukemia virus (HTLV) entry," is presented.
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- 2011
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4. Loss of Infectivity of HIV-1 Particles Produced by Mobile Lymphocytes.
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Chazal, Maxime, Nzounza, Patrycja, Pique, Claudine, and Ramirez, Bertha Cecilia
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LYMPHOCYTES , *HIV infections , *INFECTIOUS disease transmission , *T cells , *CLONING , *GENETIC engineering - Abstract
HIV-1 spreads by cell-free particles and through direct cell contacts. To discriminate between these two modes of dissemination, an assay in which the cells are cultured under shaking conditions impairing cell-to-cell transmission has been described. We addressed the impact of shaking on HIV-1 particle infectivity. Kinetics of HIV-1 infection in static or shaking conditions confirmed that HIV-1 replication is reduced in mobile lymphocyte T cells. Strikingly, the infectivity of viruses produced by mobile lymphocytes was dramatically reduced. In parallel, the amount of envelope protein present on these particles showed a continuous decrease over time. We conclude that inefficient HIV-1 replication in mobile lymphocytes in this experimental system is not only due to avoidance of viral cell-to-cell transfer but also to the loss of infectivity of the viral particles due to the alteration of the composition and functionality of the particles produced by these lymphocytes. It is important to take these observations into account when studying viral transmission under shaking conditions. [ABSTRACT FROM AUTHOR]
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- 2014
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5. Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription.
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Martella, Christophe, Tollenaere, Armelle Inge, Waast, Laetitia, Lacombe, Benoit, Groussaud, Damien, Margottin-Goguet, Florence, Ramirez, Bertha Cecilia, and Pique, Claudine
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HTLV-I , *HTLV - Abstract
Human T-cell lymphotropic virus type 1 (HTLV-1) Tax oncoprotein is required for viral gene expression. Tax transactivates the viral promoter by recruiting specific transcription factors but also by interfering with general transcription factors involved in the preinitiation step, such as TFIIA and TFIID. However, data are lacking regarding Tax interplay with TFIIH, which intervenes during the last step of preinitiation. We previously reported that XPB, the TFIIH subunit responsible for promoter opening and promoter escape, is required for Tat-induced human-immunodeficiency virus promoter transactivation. Here, we investigated whether XPB may also play a role in HTLV-1 transcription. We report that Tax and XPB directly interact in vitro and that endogenous XPB produced by HTLV-1-infected T cells binds to Tax and is recruited on proviral LTRs. In contrast, XPB recruitment at the LTR is not detected in Tax-negative HTLV-1-infected T cells and is strongly reduced when Tax-induced HTLV-1 LTR transactivation is blocked. XPB overexpression does not affect basal HTLV-1 promoter activation but enhances Tax-mediated transactivation in T cells. Conversely, downregulating XPB strongly reduces Tax-mediated transactivation. Importantly, spironolactone (SP)-mediated inhibition of LTR activation can be rescued by overexpressing XPB but not XPD, another TFIIH subunit. Furthermore, an XPB mutant defective for the ATPase activity responsible for promoter opening does not show rescue of the effect of SP. Finally, XPB downregulation reduces viability of Tax-positive but not Tax-negative HTLV-1-transformed T cell lines. These findings reveal that XPB is a novel cellular cofactor hijacked by Tax to facilitate HTLV-1 transcription. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.
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Groussaud, Damien, Khair, Mostafa, Tollenaere, Armelle I., Waast, Laetitia, Kuo, Mei-Shiue, Mangeney, Marianne, Martella, Christophe, Fardini, Yann, Coste, Solène, Souidi, Mouloud, Benit, Laurence, Pique, Claudine, and Issad, Tarik
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HTLV-I , *T cells , *HTLV , *ENZYMES , *PHOSPHORYLATION , *CREB protein , *VIRAL replication - Abstract
The viral Tax oncoprotein plays a key role in both Human T-cell lymphotropic virus type 1 (HTLV-1)-replication and HTLV-1-associated pathologies, notably adult T-cell leukemia. Tax governs the transcription from the viral 5’LTR, enhancing thereby its own expression, via the recruitment of dimers of phosphorylated CREB to cAMP-response elements located within the U3 region (vCRE). In addition to phosphorylation, CREB is also the target of O-GlcNAcylation, another reversible post-translational modification involved in a wide range of diseases, including cancers. O-GlcNAcylation consists in the addition of O-linked-N-acetylglucosamine (O-GlcNAc) on Serine or threonine residues, a process controlled by two enzymes: O-GlcNAc transferase (OGT), which transfers O-GlcNAc on proteins, and O-GlcNAcase (OGA), which removes it. In this study, we investigated the status of O-GlcNAcylation enzymes in HTLV-1-transformed T cells. We found that OGA mRNA and protein expression levels are increased in HTLV-1-transformed T cells as compared to control T cell lines while OGT expression is unchanged. However, higher OGA production coincides with a reduction in OGA specific activity, showing that HTLV-1-transformed T cells produce high level of a less active form of OGA. Introducing Tax into HEK-293T cells or Tax-negative HTLV-1-transformed TL-om1 T cells is sufficient to inhibit OGA activity and increase total O-GlcNAcylation, without any change in OGT activity. Furthermore, Tax interacts with the OGT/OGA complex and inhibits the activity of OGT-bound OGA. Pharmacological inhibition of OGA increases CREB O-GlcNAcylation as well as HTLV-1-LTR transactivation by Tax and CREB recruitment to the LTR. Moreover, overexpression of wild-type CREB but not a CREB protein mutated on a previously described O-GlcNAcylation site enhances Tax-mediated LTR transactivation. Finally, both OGT and OGA are recruited to the LTR. These findings reveal the interplay between Tax and the O-GlcNAcylation pathway and identify new key molecular actors involved in the assembly of the Tax-dependent transactivation complex. [ABSTRACT FROM AUTHOR]
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- 2017
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7. ALPK1 controls TIFA/TRAF6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria.
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Milivojevic, Milica, Dangeard, Anne-Sophie, Kasper, Christoph Alexander, Tschon, Therese, Emmenlauer, Mario, Pique, Claudine, Schnupf, Pamela, Guignot, Julie, and Arrieumerlou, Cécile
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NATURAL immunity , *GRAM-negative bacteria , *CYTOKINES , *CELL communication , *EPITHELIAL cells , *OLIGOMERIZATION - Abstract
During infection by invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. These are directly produced by infected cells or by uninfected bystanders via connexin-dependent cell-cell communication. However, the cellular pathways underlying this process remain largely unknown. Here we perform a genome-wide RNA interference screen and identify TIFA and TRAF6 as central players of Shigella flexneri and Salmonella typhimurium-induced interleukin-8 expression. We show that threonine 9 and the forkhead-associated domain of TIFA are necessary for the oligomerization of TIFA in both infected and bystander cells. Subsequently, this process triggers TRAF6 oligomerization and NF-κB activation. We demonstrate that TIFA/TRAF6-dependent cytokine expression is induced by the bacterial metabolite heptose-1,7-bisphosphate (HBP). In addition, we identify alpha-kinase 1 (ALPK1) as the critical kinase responsible for TIFA oligomerization and IL-8 expression in response to infection with S. flexneri and S. typhimurium but also to Neisseria meningitidis. Altogether, these results clearly show that ALPK1 is a master regulator of innate immunity against both invasive and extracellular gram-negative bacteria. [ABSTRACT FROM AUTHOR]
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- 2017
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8. A Non-SUMOylated Tax Protein Is Still Functional for NF-B Pathway Activation.
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Pène, Sabrina, Waast, Laetitia, Bonnet, Amandine, Bénit, Laurence, and Pique, Claudine
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SMALL ubiquitin-related modifier proteins , *SYNTAXINS , *NF-kappa B , *HTLV-I , *SMALL interfering RNA , *GENE expression - Abstract
Whether NF-κB promoter transactivation by the human T-cell leukemia virus type 1 (HTLV-1) Tax protein requires Tax SUMOylation is still a matter of debate. In this study, we revisited the role of Tax SUMOylation using a strategy based on the targeting of Ubc9, the unique E2 SUMO-conjugating enzyme. We show that either a catalytically inactive form of Ubc9 (Ubc9- C93S) or Ubc9 small interfering RNA (siRNA) dramatically reduces Tax conjugation to endogenous SUMO-1 or SUMO-2/3, demonstrating that as expected, Tax SUMOylation is under the control of the catalytic activity of Ubc9. We further report that a non-SUMOylated Tax protein produced in 293T cells is still able to activate either a transfected or an integrated NF-κB reporter promoter and to induce expression of an NF-κB-regulated endogenous gene. Importantly, blocking Ubc9 activity in T cells also results in the production of a non-SUMOylated Tax that is still fully functional for the activation of a NF-κB promoter. These results provide the definitive evidence that Tax SUMOylation is not required for NF-κB-driven gene induction. [ABSTRACT FROM AUTHOR]
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- 2014
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9. The Scaffolding Protein Dlg1 Is a Negative Regulator of Cell-Free Virus Infectivity but Not of Cell-to-Cell HIV-1 Transmission in T Cells.
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Nzounza, Patrycja, Chazal, Maxime, Guedj, Chloé, Schmitt, Alain, Massé, Jean-Marc, Randriamampita, Clotilde, Pique, Claudine, and Ramirez, Bertha Cecilia
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TISSUE scaffolds , *NEGATIVE regulatory factor , *T cells , *HIV , *CELL membranes - Abstract
Background: Cell-to-cell virus transmission of Human immunodeficiency virus type-1 (HIV-1) is predominantly mediated by cellular structures such as the virological synapse (VS). The VS formed between an HIV-1-infected T cell and a target T cell shares features with the immunological synapse (IS). We have previously identified the human homologue of the Drosophila Discs Large (Dlg1) protein as a new cellular partner for the HIV-1 Gag protein and a negative regulator of HIV-1 infectivity. Dlg1, a scaffolding protein plays a key role in clustering protein complexes in the plasma membrane at cellular contacts. It is implicated in IS formation and T cell signaling, but its role in HIV-1 cell-to-cell transmission was not studied before. Methodology/Principal Findings: Kinetics of HIV-1 infection in Dlg1-depleted Jurkat T cells show that Dlg1 modulates the replication of HIV-1. Single-cycle infectivity tests show that this modulation does not take place during early steps of the HIV-1 life cycle. Immunofluorescence studies of Dlg1-depleted Jurkat T cells show that while Dlg1 depletion affects IS formation, it does not affect HIV-1-induced VS formation. Co-culture assays and quantitative cell-to-cell HIV-1 transfer analyses show that Dlg1 depletion does not modify transfer of HIV-1 material from infected to target T cells, or HIV-1 transmission leading to productive infection via cell contact. Dlg1 depletion results in increased virus yield and infectivity of the viral particles produced. Particles with increased infectivity present an increase in their cholesterol content and during the first hours of T cell infection these particles induce higher accumulation of total HIV-1 DNA. Conclusion: Despite its role in the IS formation, Dlg1 does not affect the VS and cell-to-cell spread of HIV-1, but plays a role in HIV-1 cell-free virus transmission. We propose that the effect of Dlg1 on HIV-1 infectivity is at the stage of virus entry. [ABSTRACT FROM AUTHOR]
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- 2012
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10. Low nuclear body formation and tax SUMOylation do not prevent NF-kappaB promoter activation.
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Bonnet, Amandine, Randrianarison-Huetz, Voahangy, Nzounza, Patrycja, Nedelec, Martine, Chazal, Maxime, Waast, Laetitia, Pene, Sabrina, Bazarbachi, Ali, Mahieux, Renaud, B‚nit, Laurence, and Pique, Claudine
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PROTEINS , *BIOSYNTHESIS , *AMINO acids , *LYMPHOCYTES , *B cells - Abstract
Background: The Tax protein encoded by Human T-lymphotropic virus type 1 (HTLV-1) is a powerful activator of the NF-κB pathway, a property critical for HTLV-1-induced immortalization of CD4+ T lymphocytes. Tax permanently stimulates this pathway at a cytoplasmic level by activating the I-κB kinase (IKK) complex and at a nuclear level by enhancing the binding of the NF-κB factor RelA to its cognate promoters and by forming nuclear bodies, believed to represent transcriptionally active structures. In previous studies, we reported that Tax ubiquitination and SUMOylation play a critical role in Tax localization and NF-κB activation. Indeed, analysis of lysine Tax mutants fused or not to ubiquitin or SUMO led us to propose a two-step model in which Tax ubiquitination first intervenes to activate IKK while Tax SUMOylation is subsequently required for promoter activation within Tax nuclear bodies. However, recent studies showing that ubiquitin or SUMO can modulate Tax activities in either the nucleus or the cytoplasm and that SUMOylated Tax can serve as substrate for ubiquitination suggested that Tax ubiquitination and SUMOylation may mediate redundant rather than successive functions. Results: In this study, we analyzed the properties of a new Tax mutant that is properly ubiquitinated, but defective for both nuclear body formation and SUMOylation. We report that reducing Tax SUMOylation and nuclear body formation do not alter the ability of Tax to activate IKK, induce RelA nuclear translocation, and trigger gene expression from a NF-κB promoter. Importantly, potent NF-κB promoter activation by Tax despite low SUMOylation and nuclear body formation is also observed in T cells, including CD4+ primary T lymphocytes. Moreover, we show that Tax nuclear bodies are hardly observed in HTLV-1-infected T cells. Finally, we provide direct evidence that the degree of NF-κB activation by Tax correlates with the level of Tax ubiquitination, but not SUMOylation. Conclusions: These data reveal that the formation of Tax nuclear bodies, previously associated to transcriptional activities in Tax-transfected cells, is dispensable for NF-κB promoter activation, notably in CD4+ T cells. They also provide the first evidence that Tax SUMOylation is not a key determinant for Tax-induced NF-κB activation. [ABSTRACT FROM AUTHOR]
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- 2012
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11. Molecular Aspects of HTLV-1 Entry: Functional Domains of the HTLV-1 Surface Subunit (SU) and Their Relationships to the Entry Receptors.
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Jones, Kathryn S., Lambert, Sophie, Bouttier, Manuella, Bénit, Laurence, Ruscetti, Frank W., Hermine, Olivier, and Pique, Claudine
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HTLV-I , *RETROVIRUSES , *PROTEOGLYCANS , *GLYCOPROTEINS , *NEUROPILINS , *CELL adhesion molecules - Abstract
The initial step in retroviral infection involves specific interactions between viral envelope proteins (Env) and specific receptors on the surface of target cells. For many years, little was known about the entry receptors for HTLV-1. During this time, however, functional domains of the HTLV-1 Env were identified by analyzing the effects of neutralizing antibodies and specific mutations in Env on HTLV-1 infectivity. More recent studies have revealed that HTLV-1 infectivity involves interactions with three different molecules: heparan sulfate proteoglycans (HSPG), the VEGF-165 receptor Neuropilin 1 (NRP-1) and glucose transporter type 1 (GLUT1). Here, we revisit previously published data on the functional domains of Env in regard to the recent knowledge acquired about this multi-receptor complex. We also discuss the similarities and differences between HTLV-1 and other deltaretroviruses in regards to receptor usage. [ABSTRACT FROM AUTHOR]
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- 2011
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12. Current concepts regarding the HTLV-1 receptor complex.
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Ghez, David, Lepelletier, Yves, Jones, Kathryn S., Pique, Claudine, and Hermine, Olivier
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HTLV-I , *GLUCOSE , *NEUROPILINS , *CELL adhesion molecules , *PROTEOGLYCANS - Abstract
The identity of the Human T lymphotropic Virus type 1 (HTLV-1) receptor remained an unsolved puzzle for two decades, until the recent demonstration that three molecules, Glucose Transporter 1, Neuropilin-1 and Heparan Sulfate Proteoglycans are involved in HTLV-1 binding and entry. Despite these advances, several questions remain unanswered, including the precise role of each of these molecules during virus entry. In light of the most recent data, we propose a model of the HTLV-1 receptor complex and discuss its potential impact on HTLV-1 infection. [ABSTRACT FROM AUTHOR]
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- 2010
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13. A role for CD81 on the late steps of HIV-1 replication in a chronically infected T cell line.
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Grigorov, Boyan, Attuil-Audenis, Valérie, Perugi, Fabien, Nedelec, Martine, Watson, Sarah, Pique, Claudine, Darlix, Jean-Luc, Conjeaud, Hélène, and Muriaux, Delphine
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HIV , *VIRAL replication , *VIRION , *IMMUNOGLOBULINS , *CELL membranes , *T cells , *RNA - Abstract
Background: HIV-1 uses cellular co-factors for virion formation and release. The virus is able to incorporate into the viral particles host cellular proteins, such as tetraspanins which could serve to facilitate HIV-1 egress. Here, we investigated the implication of several tetraspanins on HIV-1 formation and release in chronically infected T-lymphoblastic cells, a model that permits the study of the late steps of HIV-1 replication. Results: Our data revealed that HIV-1 Gag and Env structural proteins co-localized with tetraspanins in the form of clusters. Co-immunoprecipitation experiments showed that Gag proteins interact, directly or indirectly, with CD81, and less with CD82, in tetraspanin-enriched microdomains composed of CD81/CD82/CD63. In addition, when HIV-1 producing cells were treated with anti-CD81 antibodies, or upon CD81 silencing by RNA interference, HIV-1 release was significantly impaired, and its infectivity was modulated. Finally, CD81 downregulation resulted in Gag redistribution at the cell surface. Conclusion: Our findings not only extend the notion that HIV-1 assembly can occur on tetraspanin-enriched microdomains in T cells, but also highlight a critical role for the tetraspanin CD81 on the late steps of HIV replication. [ABSTRACT FROM AUTHOR]
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- 2009
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14. Alteration of Blood-Brain Barrier Integrity by Retroviral Infection.
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Afonso, Philippe V., Ozden, Simona, Cumont, Marie-Christine, Seilhean, Danielle, Cartier, Luis, Rezaie, Payam, Mason, Sarah, Lambert, Sophie, Huerre, Michel, Gessain, Antoine, Couraud, Pierre-Olivier, Pique, Claudine, Ceccaldi, Pierre-Emmanuel, and Romero, Ignacio A.
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BLOOD-brain barrier disorders , *RETROVIRUS diseases , *NEURODEGENERATION , *ENDOTHELIUM diseases , *DISEASE susceptibility , *ASTROCYTES - Abstract
The blood-brain barrier (BBB), which forms the interface between the blood and the cerebral parenchyma, has been shown to be disrupted during retroviral-associated neuromyelopathies. Human T Lymphotropic Virus (HTLV-1) Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is a slowly progressive neurodegenerative disease associated with BBB breakdown. The BBB is composed of three cell types: endothelial cells, pericytes and astrocytes. Although astrocytes have been shown to be infected by HTLV-1, until now, little was known about the susceptibility of BBB endothelial cells to HTLV-1 infection and the impact of such an infection on BBB function. We first demonstrated that human cerebral endothelial cells express the receptors for HTLV-1 (GLUT-1, Neuropilin-1 and heparan sulfate proteoglycans), both in vitro, in a human cerebral endothelial cell line, and ex vivo, on spinal cord autopsy sections from HAM/TSP and non-infected control cases. In situ hybridization revealed HTLV-1 transcripts associated with the vasculature in HAM/TSP. We were able to confirm that the endothelial cells could be productively infected in vitro by HTLV-1 and that blocking of either HSPGs, Neuropilin 1 or Glut1 inhibits this process. The expression of the tight-junction proteins within the HTLV-1 infected endothelial cells was altered. These cells were no longer able to form a functional barrier, since BBB permeability and lymphocyte passage through the monolayer of endothelial cells were increased. This work constitutes the first report of susceptibility of human cerebral endothelial cells to HTLV-1 infection, with implications for HTLV-1 passage through the BBB and subsequent deregulation of the central nervous system homeostasis. We propose that the susceptibility of cerebral endothelial cells to retroviral infection and subsequent BBB dysfunction is an important aspect of HAM/TSP pathogenesis and should be considered in the design of future therapeutics strategies. [ABSTRACT FROM AUTHOR]
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- 2008
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15. The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking.
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Blot, Vincent, Lopez-Vergès, Sandra, Breton, Marie, Pique, Claudine, Berlioz-Torrent, Clarisse, and Grange, Marie-Pierre
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GLYCOPROTEINS , *RETROVIRUSES , *PROTEINS , *CYTOPLASM , *MOUSE leukemia viruses , *CELLS - Abstract
Background: Retrovirus particles emerge from the assembly of two structural protein components, Gag that is translated as a soluble protein in the cytoplasm of the host cells, and Env, a type I transmembrane protein. Because both components are translated in different intracellular compartments, elucidating the mechanisms of retrovirus assembly thus requires the study of their intracellular trafficking. Results: We used a CD25 (Tac) chimera-based approach to study the trafficking of Moloney murine leukemia virus and Mason-Pfizer monkey virus Env proteins. We found that the cytoplasmic tails (CTs) of both Env conserved two major signals that control a complex intracellular trafficking. A dileucine-based motif controls the sorting of the chimeras from the trans-Golgi network (TGN) toward endosomal compartments. Env proteins then follow a retrograde transport to the TGN due to the action of a tyrosine-based motif. Mutation of either motif induces the mis-localization of the chimeric proteins and both motifs are found to mediate interactions of the viral CTs with clathrin adaptors. Conclusion: This data reveals the unexpected complexity of the intracellular trafficking of retrovirus Env proteins that cycle between the TGN and endosomes. Given that Gag proteins hijack endosomal host proteins, our work suggests that the endosomal pathway may be used by retroviruses to ensure proper encountering of viral structural Gag and Env proteins in cells, an essential step of virus assembly. [ABSTRACT FROM AUTHOR]
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- 2006
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16. Stable Ubiquitination of Human T-Cell Leukemia Virus Type 1 Tax Is Required for Proteasome Binding.
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Chiari, Estelle, Lamsoul, Isabelle, Lodewick, Julie, Chopin, Cécile, Bex, Françoise, and Pique, Claudine
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UBIQUITIN , *LEUKEMIA , *T cells , *VIROLOGY , *MICROBIOLOGY , *MICROORGANISMS - Abstract
Human T-cell leukemia virus type 1 (HTLV-1) is the retrovirus responsible for adult T-cell leukemia and HTLV-1-associated myelopathy. Adult T-cell leukemia development is mainly due to the ability of the viral oncoprotein Tax to promote T-cell proliferation, whereas the appearance of HTLV-1-associated myelopathy involves the antigenic properties of Tax. Understanding the events regulating the intracellular level of Tax is therefore an important issue. How Tax is degraded has not been determined, but it is known that Tax binds to proteasomes, the major sites for degradation of intracellular proteins, generally tagged through polyubiquitin conjugation. In this study, we investigated the relationship between Tax, ubiquitin, and proteasomes. We report that mono- and polyubiquitinated Tax proteins can be recovered from both transfected 293T cells and T lymphocytes. We also show that lysine residues located in the carboxy-terminal domain of Tax are the principal targets of this process. Remarkably, we further demonstrate that mutation of lysine residues in the C-terminal part of Tax, which massively reduces Tax ubiquitination, impairs proteasome binding, and conversely, that a Tax mutant that binds poorly to this particle (M22) is faintly ubiquitinated, suggesting that Tax ubiquitination is required for association with cellular proteasomes. Finally, we document that comparable amounts of ubiquitinated species were found whether proteasome activities were inhibited or not, providing evidence that they are not directly addressed to proteasomes for degradation. These findings indicate that although it is ubiquitinated and binds to proteasomes, Tax is not massively degraded via the ubiquitin-proteasome pathway and therefore reveal that Tax conjugation to ubiquitin mediates a nonproteolytic function. [ABSTRACT FROM AUTHOR]
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- 2004
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17. Direct Evidence for a Chronic CD8+-T-Cell-Medicated Immune Reaction to Tax within the Muscle of a Human T-Cell Leukemia/Lymphoma Virus Type 1-Infected Patient with Sporadic Inclusion Body Myositis.
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Ozden, Simona, Cochet, Madeleine, Mikol, Jacqueline, Teixeira, Antonio, Gessain, Antoine, and Pique, Claudine
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HTLV diseases , *ANTINEOPLASTIC agents , *HTLV , *RETROVIRUSES , *MYOSITIS , *T cells , *VIROLOGY , *IMMUNOLOGY - Abstract
Human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection can lead to the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), concomitantly with or without other inflammatory disorders such as myositis. These pathologies are considered immune-mediated diseases, and it is assumed that migration within tissues of both HTLV-1-infected CD4+ T cells and anti-HTLV-1 cytotoxic T cells represents a pivotal event. However, although HTLV-1-infected T cells were found in inflamed lesions, the antigenic specificity of coinfiltrated CD8+ T cells remains to be determined. In this study, we performed both ex vivo and in situ analyses using muscle biopsies obtained from an HTLV-1-infected patient with HAM/TSP and sporadic inclusion body myositis. We found that both HTLV-1-infected CD4+ T cells and CD8+ T cells directed to the dominant Tax antigen can be amplified from muscle cell cultures. Moreover, we were able to detect in two successive muscle biopsies both tax mRNA-positive mononuclear cells and T cells recognized by the Tax11-19/HLA-A*02 tetramer and positive for perforin. These findings provide the first direct demonstration that anti-Tax cytotoxic T cells are chronically recruited within inflamed tissues of an HTLV-1 infected patient, which validates the cytotoxic immune reaction model for the pathogenesis of HTLV-1-associated inflammatory disease. [ABSTRACT FROM AUTHOR]
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- 2004
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18. Human Dlg protein binds to the envelope glycoproteins of human T-cell leukemia virus type 1 and regulates envelope mediated cell-cell fusion in T lymphocytes.
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Blot, Vincent, Delamarre, Lélia, Perugi, Fabien, Pham, Danielle, Bénichou, Serge, Benarous, Richard, Hanada, Toshihiko, Chishti, Athar H., Dokhélar, Marie-Christine, and Pique, Claudine
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TUMOR suppressor proteins , *CELL fusion , *T cells , *RETROVIRUSES , *GLYCOPROTEINS - Abstract
Human homologue of the Drosophila Dig tumor suppressor (hDlg) is a widely expressed scaffold protein implicated in the organization of multi-protein complexes at cell adhesion sites such as the neuronal synapse, hDlg contains three PDZ domains that mediate its binding to the consensus motifs present at the C-termini of various cell surface proteins, thus inducing their clustering and/or stabilization at the plasma membrane. Using a yeast two- hybrid screen, we identified hDlg as a cellular binding partner of a viral membrane integral protein, the envelope glycoprotein (Env) of human T-cell leukemia virus type 1 (HTLV-1). HTLV-1 is a human retrovirus that infects CD4+ T lymphocytes and is preferentially transmitted via direct contacts between infected and target cells, through a structure referred to as the virological synapse. Here, we demonstrate that hDlg interacts with a classical PDZ domain-binding motif present at the C-terminus of the cytoplasmic domain of HTLV-1 Env and conserved in the related HTLV-2 virus. We further document that, in HTLV- 1 infected primary T cells, hDlg and Env are concentrated in restricted areas of the plasma membrane, enriched in molecules involved in T-cell contacts. The presence of Gag proteins responsible for viral assembly and budding in these areas indicated that they constitute platforms for viral assembly and transmission. Finally, a mutant virus unable to bind hDlg exhibited a decreased ability to trigger Env mediated cell fusion between T lymphocytes. We thus propose that hDlg stabilizes HTLV-1 envelope glycoproteins at the virological synapse formed between infected and target cells, hence assisting the cell-to-cell transmission of the virus. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
19. Nedd4.1-mediated ubiquitination and subsequent recruitment of Tsg101 ensure HTLV-1 Gag trafficking toward the multivesicular body pathway prior to virus budding.
- Author
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Blot, Vincent, Perugi, Fabien, Gay, Bernard, Prévost, Marie-Christine, Briant, Laurence, Tangy, Fréderic, Abriel, Hugues, Staubb, Olivier, Dokhélar, Marie-Christine, and Pique, Claudine
- Subjects
- *
RETROVIRUSES , *PROTEINS , *CELL membranes , *BUDDING (Zoology) , *UBIQUITIN , *HIV - Abstract
One of the most exciting recent developments in the field of retroviruses is the finding that their Gag proteins hijack cellular proteins from the mutivesicular body (MVB) pathway during the budding process. The Gag proteins of oncoretroviruses possess a PPxY motif that recruits a ubiquitin ligase from the Nedd4 family, whereas those of the human immunodeficiency virus interact through a TAP motif with Tsgl01, a protein of the ESCRT-1 complex. It is currently assumed that Nedd4 and Tsg101 represent equivalent entry gates towards the same cellular process leading to budding, and that both partners are recruited to the plasma membrane where viral budding occurs. However, we report here that the budding of the fireman oncoretrovirus HTLV-1, the Gag proteins of which Hess tandem PPPY/PTAP motifs, requires both Nedd4 and Tsgl01. We show that Nedd4.1, but not Nedd4.2, is recruited by the PPPY motif of Gag and subsequently catalyzes Gag ubiquitination. We also demonstrate that Gag interacts first with Nedd4.1 at the plasma membrane and then with Tsg101 in late endosomes/MVBs. Consistently, we found that HTLV-1 particles mutated in the PPPY motif remain underneath the plasma membrane, blocked at an early step of the budding process, whereas PTAP-mutated viruses accumulate in intracellular vesicles, blocked at a later step. Our findings indicate that Nedd4.1 and Tsg101 act successively in the assembly process of HTLV-1 to ensure proper Gag trafficking through the endocytic pathway up to late endosomes where the late steps of retroviral release occur. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
20. Xylosyltransferase 2, a protein encoded on chromosome 17q, is involved in HTLV entry.
- Author
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Jensen, Stig M. R., Jones, Kathryn S., Bénit, Laurence, Bertolette, Daniel C., Petrow-Sadowski, Cari, Ruscetti, Francis W., and Pique, Claudine
- Subjects
- *
HTLV , *PROTEIN analysis - Abstract
An abstract of the research paper titled,"Xylosyltransferase 2, a protein encoded on chromosome 17q, is involved in the Human T-cell leukemia virus (HTLV) entry," is presented.
- Published
- 2011
- Full Text
- View/download PDF
21. Predominant role of Tax sumoylation in Tax-induced NF-kB activation in T cells.
- Author
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Bonnet, Amandine, Favre-Bonvin, Arnaud, Nzounza, Patrycja, Nedelec, Martine, Chazal, Maxime, Waast, Laetitia, Randrianarison, Voahangy, Bazarbachi, Ali, Mahieux, Renaud, Benit, Laurence, and Pique, Claudine
- Subjects
- *
NF-kappa B , *T cells - Abstract
An abstract of the research paper titled,"Predominant role of Tax sumoylation in Tax-induced NF-kB activation in T cells," is presented.
- Published
- 2011
- Full Text
- View/download PDF
22. Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome.
- Author
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Kfoury, Youmna, Setterblad, Niclas, El-Sabban, Marwan, Zamborlini, Alessia, Dassouki, Zeina, Hajj, Hiba El, Hermine, Olivier, Pique, Claudine, de Thé, Hugues, Saïb, Ali, and Bazarbachi, Ali
- Subjects
- *
HTLV , *PROTEIN synthesis - Abstract
An abstract of the research paper titled,"Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome ," is presented.
- Published
- 2011
- Full Text
- View/download PDF
23. Exclusion from the Golgi and very low levels of HTLV-2 Tax ubiquitination do not prevent IKK-gamma/NEMO relocalization and NF-κB activation.
- Author
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Journo, Chloé, Bonnet, Amandine, Favre-Bonvin, Arnaud, Turpin, Jocelyn, Chevalier, Sébastien, Vinera, Jennifer, Côté, Emilie, Pique, Claudine, and Mahieux, Renaud
- Subjects
- *
HTLV , *NF-kappa B - Abstract
An abstract of the research paper titled, "Exclusion from the Golgi and very low levels of Human T-cell leukemia virus type 2( HTLV-2) Tax ubiquitination do not prevent IKK-gamma/NEMO relocalization and NF-kB activation," is presented.
- Published
- 2011
- Full Text
- View/download PDF
24. Neuropilin-1 Is Involved in Human T-Cell Lymphotropic Virus Type 1 Entry.
- Author
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Ghez, David, Lepelletier, Yves, Lambert, Sophie, Fourneau, Jean-Marie, Blot, Vincent, Janvier, Sébastien, Arnulf, Bertrand, Van Endert, Peter M., Heveker, Nikolaus, Pique, Claudine, and Hermine, Olivier
- Subjects
- *
T cells , *VIRUSES , *GLUCOSE , *NEUROPILINS , *PROTEINS - Abstract
Human T-cell lymphotropic virus type 1 (HTLV-1) is transmitted through a viral synapse and enters target cells via interaction with the glucose transporter GLUT1. Here, we show that Neuropilin-1 (NRP1), the receptor for semaphorin-3A and VEGF-A165 and a member of the immune synapse, is also a physical and functional partner of HTLV-1 envelope (Env) proteins. HTLV-1 Env and NRP1 complexes are formed in cotransfected cells, and endogenous NRP1 contributes to the binding of HTLV-1 Env to target cells. NRP1 overexpression increases HTLV-1 Env-dependent syncytium formation. Moreover, overexpression of NRP1 increases both HTLV-1 and HTLV-2 Env-dependent infection, whereas down-regulation of endogenous NRP1 has the opposite effect. Finally, overexpressed GLUT1, NRP1, and Env form ternary complexes in transfected cells, and endogenous NRP1 and GLUT1 colocalize in membrane junctions formed between uninfected and HTLV-1-infected T cells. These data show that NRP1 is involved in HTLV-1 and HTLV-2 entry, suggesting that the HTLV receptor has a multicomponent nature. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
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