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302 results on '"Pirazzini, Chiara"'

1. Plasma proteomics identify biomarkers predicting Parkinson’s disease up to 7 years before symptom onset

Author

Hällqvist, Jenny, Bartl, Michael, Dakna, Mohammed, Schade, Sebastian, Garagnani, Paolo, Bacalini, Maria-Giulia, Pirazzini, Chiara, Bhatia, Kailash, Schreglmann, Sebastian, Xylaki, Mary, Weber, Sandrina, Ernst, Marielle, Muntean, Maria-Lucia, Sixel-Döring, Friederike, Franceschi, Claudio, Doykov, Ivan, Śpiewak, Justyna, Vinette, Héloїse, Trenkwalder, Claudia, Heywood, Wendy E., Mills, Kevin, and Mollenhauer, Brit

Published
2024
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2. Centenarian clocks: epigenetic clocks for validating claims of exceptional longevity

Author

Dec, Eric, Clement, James, Cheng, Kaiyang, Church, George M, Fossel, Michael B, Rehkopf, David H, Rosero-Bixby, Luis, Kobor, Michael S, Lin, David TS, Lu, Ake T, Fei, Zhe, Guo, Wei, Chew, Yap Ching, Yang, Xiaojing, Putra, Sulistyo E Dwi, Reiner, Alex P, Correa, Adolfo, Vilalta, Adrian, Pirazzini, Chiara, Passarino, Giuseppe, Monti, Daniela, Arosio, Beatrice, Garagnani, Paolo, Franceschi, Claudio, and Horvath, Steve

Subjects
Biological Sciences, Genetics, Aging, Clinical Research, Human Genome, Aged, 80 and over, Humans, Longevity, Centenarians, DNA Methylation, Epigenesis, Genetic, Centenarian clocks, Epigenetic clocks, Clinical sciences
Abstract

Claims surrounding exceptional longevity are sometimes disputed or dismissed for lack of credible evidence. Here, we present three DNA methylation-based age estimators (epigenetic clocks) for verifying age claims of centenarians. The three centenarian clocks were developed based on n = 7039 blood and saliva samples from individuals older than 40, including n = 184 samples from centenarians, 122 samples from semi-supercentenarians (aged 105 +), and 25 samples from supercentenarians (aged 110 +). The oldest individual was 115 years old. Our most accurate centenarian clock resulted from applying a neural network model to a training set composed of individuals older than 40. An epigenome-wide association study of age in different age groups revealed that age effects in young individuals (age  90). We present a chromatin state analysis of age effects in centenarians. The centenarian clocks are expected to be useful for validating claims surrounding exceptional old age.

Published
2023

3. Development and validation of cardiometabolic risk predictive models based on LDL oxidation and candidate geromarkers from the MARK-AGE data

Author

Valeanu, Andrei, Margina, Denisa, Weber, Daniela, Stuetz, Wolfgang, Moreno-Villanueva, María, Dollé, Martijn E.T., Jansen, Eugène HJM, Gonos, Efstathios S., Bernhardt, Jürgen, Grubeck-Loebenstein, Beatrix, Weinberger, Birgit, Fiegl, Simone, Sikora, Ewa, Mosieniak, Grazyna, Toussaint, Olivier, Debacq-Chainiaux, Florence, Capri, Miriam, Garagnani, Paolo, Pirazzini, Chiara, Bacalini, Maria Giulia, Hervonen, Antti, Slagboom, P. Eline, Talbot, Duncan, Breusing, Nicolle, Frank, Jan, Bürkle, Alexander, Franceschi, Claudio, Grune, Tilman, and Gradinaru, Daniela

Published
2024
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6. CAR+ extracellular vesicles predict ICANS in patients with B cell lymphomas treated with CD19-directed CAR T cells

Author

Storci, Gianluca, primary, De Felice, Francesco, additional, Ricci, Francesca, additional, Santi, Spartaco, additional, Messelodi, Daria, additional, Bertuccio, Salvatore Nicola, additional, Laprovitera, Noemi, additional, Dicataldo, Michele, additional, Rossini, Lucrezia, additional, De Matteis, Serena, additional, Casadei, Beatrice, additional, Vaglio, Francesca, additional, Ursi, Margherita, additional, Barbato, Francesco, additional, Roberto, Marcello, additional, Guarino, Maria, additional, Asioli, Gian Maria, additional, Arpinati, Mario, additional, Cortelli, Pietro, additional, Maffini, Enrico, additional, Tomassini, Enrica, additional, Tassoni, Marta, additional, Cavallo, Carola, additional, Iannotta, Francesco, additional, Naddeo, Maria, additional, Tazzari, Pier Luigi, additional, Dan, Elisa, additional, Pellegrini, Cinzia, additional, Guadagnuolo, Serafina, additional, Carella, Matteo, additional, Sinigaglia, Barbara, additional, Pirazzini, Chiara, additional, Severi, Caterina, additional, Garagnani, Paolo, additional, Kwiatkowska, Katarzyna Malgorzata, additional, Ferracin, Manuela, additional, Zinzani, Pier Luigi, additional, Bonafè, Massimiliano, additional, and Bonifazi, Francesca, additional

Published
2024
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8. Early downregulation of hsa-miR-144-3p in serum from drug-naïve Parkinson’s disease patients

Author

Zago, Elisa, Dal Molin, Alessandra, Dimitri, Giovanna Maria, Xumerle, Luciano, Pirazzini, Chiara, Bacalini, Maria Giulia, Maturo, Maria Giovanna, Azevedo, Tiago, Spasov, Simeon, Gómez-Garre, Pilar, Periñán, María Teresa, Jesús, Silvia, Baldelli, Luca, Sambati, Luisa, Calandra-Buonaura, Giovanna, Garagnani, Paolo, Provini, Federica, Cortelli, Pietro, Mir, Pablo, Trenkwalder, Claudia, Mollenhauer, Brit, Franceschi, Claudio, Liò, Pietro, and Nardini, Christine

Published
2022
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11. A geroscience approach for Parkinson’s disease: Conceptual framework and design of PROPAG-AGEING project

Author

Adarmes-Gómez, Astrid, Azevedo, Tiago, Bacalini, Maria Giulia, Baldelli, Luca, Bartoletti-Stella, Anna, Bhatia, Kailash P., Bonilla-Toribio, Marta, Boninsegna, Claudia, Broli, Marcella, Buiza-Rueda, Dolores, Calandra-Buonaura, Giovanna, Capellari, Sabina, Carrión-Claro, Mario, Cilea, Rosalia, Clayton, Robert, Cortelli, Pietro, Molin, Alessandra Dal, De Luca, Silvia, De Massis, Patrizia, Dimitri, Giovanna Maria, Doykov, Ivan, Escuela-Martin, Rocio, Fabbri, Giovanni, Franceschi, Claudio, Gabellini, Anna, Garagnani, Paolo, Giuliani, Cristina, Gómez-Garre, Pilar, Guaraldi, Pietro, Hägg, Sara, Hällqvist, Jenny, Halsband, Claire, Heywood, Wendy, Houlden, Henry, Huertas, Ismae, Jesús, Silvia, Jylhävä, Juulia, Labrador-Espinosa, Miguel A., Licari, Cristina, Liò, Pietro, Luchinat, Claudio, Macias, Daniel, Macrì, Stefania, Magrinelli, Francesca, Rodríguez, Juan Francisco Martín, Delledonne, Massimo, Maturo, Maria Giovanna, Mengozzi, Giacomo, Meoni, Gaia, Mignani, Francesco, Milazzo, Maddalena, Mills, Kevin, Mir, Pablo, Mollenhauer, Brit, Nardini, Christine, Nassetti, Stefania Alessandra, Pedersen, Nancy L., Periñán-Tocino, Maria Teresa, Pirazzini, Chiara, Provini, Federica, Ravaioli, Francesco, Sala, Claudia, Sambati, Luisa, Scaglione, Cesa Lorella Maria, Schade, Sebastian, Schreglmann, Sebastian, Spasov, Simeon, Strom, Stephen, Tejera-Parrado, Cristina, Tenori, Leonardo, Trenkwalder, Claudia, Turano, Paola, Valzania, Franco, Ortega, Rosario Vigo, Williams, Dylan, Xumerle, Luciano, Zago, Elisa, Dal Molin, Alessandra, and Kwiatkowska, Katarzyna Malgorzata

Published
2021
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12. Adipo-Epithelial Transdifferentiation in In Vitro Models of the Mammary Gland.

Author

Perugini, Jessica, Smorlesi, Arianna, Acciarini, Samantha, Mondini, Eleonora, Colleluori, Georgia, Pirazzini, Chiara, Kwiatkowska, Katarzyna Malgorzata, Garagnani, Paolo, Franceschi, Claudio, Zingaretti, Maria Cristina, Dani, Christian, Giordano, Antonio, and Cinti, Saverio

Subjects
MAMMARY glands, MULTIPOTENT stem cells, ADIPOGENESIS, MILK proteins, WHEY proteins, EXTRACELLULAR matrix, PROTEIN expression
Abstract

Subcutaneous adipocytes are crucial for mammary gland epithelial development during pregnancy. Our and others' previous data have suggested that adipo-epithelial transdifferentiation could play a key role in the mammary gland alveolar development. In this study, we tested whether adipo-epithelial transdifferentiation occurs in vitro. Data show that, under appropriate co-culture conditions with mammary epithelial organoids (MEOs), mature adipocytes lose their phenotype and acquire an epithelial one. Interestingly, even in the absence of MEOs, extracellular matrix and diffusible growth factors are able to promote adipo-epithelial transdifferentiation. Gene and protein expression studies indicate that transdifferentiating adipocytes exhibit some characteristics of milk-secreting alveolar glands, including significantly higher expression of milk proteins such as whey acidic protein and β-casein. Similar data were also obtained in cultured human multipotent adipose-derived stem cell adipocytes. A miRNA sequencing experiment on the supernatant highlighted mir200c, which has a well-established role in the mesenchymal–epithelial transition, as a potential player in this phenomenon. Collectively, our data show that adipo-epithelial transdifferentiation can be reproduced in in vitro models where this phenomenon can be investigated at the molecular level. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Accelerated epigenetic aging in Down syndrome

Author

Horvath, Steve, Garagnani, Paolo, Bacalini, Maria Giulia, Pirazzini, Chiara, Salvioli, Stefano, Gentilini, Davide, Di Blasio, Anna Maria, Giuliani, Cristina, Tung, Spencer, Vinters, Harry V, and Franceschi, Claudio

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Genetics, Intellectual and Developmental Disabilities (IDD), Down Syndrome, Neurosciences, Brain Disorders, Aging, Congenital, Adult, Aged, Biomarkers, DNA Methylation, Epigenesis, Genetic, Epigenomics, Humans, Middle Aged, Young Adult, biomarker of aging, DNA methylation, Down syndrome, epigenetics, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Down Syndrome (DS) entails an increased risk of many chronic diseases that are typically associated with older age. The clinical manifestations of accelerated aging suggest that trisomy 21 increases the biological age of tissues, but molecular evidence for this hypothesis has been sparse. Here, we utilize a quantitative molecular marker of aging (known as the epigenetic clock) to demonstrate that trisomy 21 significantly increases the age of blood and brain tissue (on average by 6.6 years, P = 7.0 × 10(-14)).

Published
2015

15. Proteomics and machine learning identify a distinct biomarker panel to detect prodromal and early Parkinson’s disease

Author

Bartl, Michael, primary, Hällqvist, Jenny, additional, Dakna, Mohammed, additional, Schade, Sebastian, additional, Garagani, Paolo, additional, Bacalini, Maria-Giulia, additional, Pirazzini, Chiara, additional, Bhatia, Kailash, additional, Schreglmann, Sebastian, additional, Xylaki, Mary, additional, Weber, Sandrina, additional, Ernst, Marielle, additional, Muntean, Maria-Lucia, additional, Sixel-Döring, Friederike, additional, Franceschi, Claudio, additional, Doykov, Ivan, additional, Trenkwalder, Claudia, additional, Heywood, Wendy, additional, Mills, Kevin, additional, and Mollenhauer, Brit, additional

Published
2023
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16. Genomic history of the Italian population recapitulates key evolutionary dynamics of both Continental and Southern Europeans

Author

Sazzini, Marco, Abondio, Paolo, Sarno, Stefania, Gnecchi-Ruscone, Guido Alberto, Ragno, Matteo, Giuliani, Cristina, De Fanti, Sara, Ojeda-Granados, Claudia, Boattini, Alessio, Marquis, Julien, Valsesia, Armand, Carayol, Jerome, Raymond, Frederic, Pirazzini, Chiara, Marasco, Elena, Ferrarini, Alberto, Xumerle, Luciano, Collino, Sebastiano, Mari, Daniela, Arosio, Beatrice, Monti, Daniela, Passarino, Giuseppe, D’Aquila, Patrizia, Pettener, Davide, Luiselli, Donata, Castellani, Gastone, Delledonne, Massimo, Descombes, Patrick, Franceschi, Claudio, and Garagnani, Paolo

Published
2020
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19. Heterogeneity of Cellular Senescence: Cell Type-Specific and Senescence Stimulus-Dependent Epigenetic Alterations

Author

Kwiatkowska, Katarzyna Malgorzata, primary, Mavrogonatou, Eleni, additional, Papadopoulou, Adamantia, additional, Sala, Claudia, additional, Calzari, Luciano, additional, Gentilini, Davide, additional, Bacalini, Maria Giulia, additional, Dall’Olio, Daniele, additional, Castellani, Gastone, additional, Ravaioli, Francesco, additional, Franceschi, Claudio, additional, Garagnani, Paolo, additional, Pirazzini, Chiara, additional, and Kletsas, Dimitris, additional

Published
2023
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20. Regulatory T cells from patients with end-stage organ disease can be isolated, expanded and cryopreserved according good manufacturing practice improving their function

Author

Ulbar, Francesca, Montemurro, Tiziana, Jofra, Tatiana, Capri, Miriam, Comai, Giorgia, Bertuzzo, Valentina, Lavazza, Cristiana, Mandelli, Alessandra, Viganò, Mariele, Budelli, Silvia, Bacalini, Maria Giulia, Pirazzini, Chiara, Garagnani, Paolo, Giudice, Valeria, Sollazzo, Daria, Curti, Antonio, Arpinati, Mario, La Manna, Gaetano, Cescon, Matteo, Pinna, Antonio Daniele, Franceschi, Claudio, Battaglia, Manuela, Giordano, Rosaria, Catani, Lucia, and Lemoli, Roberto Massimo

Published
2019
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22. A Targeted Epigenetic Clock for the Prediction of Biological Age

Author

Gensous, Noémie, primary, Sala, Claudia, additional, Pirazzini, Chiara, additional, Ravaioli, Francesco, additional, Milazzo, Maddalena, additional, Kwiatkowska, Katarzyna Malgorzata, additional, Marasco, Elena, additional, De Fanti, Sara, additional, Giuliani, Cristina, additional, Pellegrini, Camilla, additional, Santoro, Aurelia, additional, Capri, Miriam, additional, Salvioli, Stefano, additional, Monti, Daniela, additional, Castellani, Gastone, additional, Franceschi, Claudio, additional, Bacalini, Maria Giulia, additional, and Garagnani, Paolo, additional

Published
2022
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26. Transcriptomic analysis reveals an association of FCGBP with Parkinson’s disease

Author

Gómez Garre, Pilar, Periñán Tocino, María Teresa, Jesús, Silvia, Bacalini, Maria Giulia, Garagnani, Paolo, Mollenhauer, Brit, Pirazzini, Chiara, Provini, Federica, Trenkwalder, Claudia, Franceschi, Claudio, Mir Rivera, Pablo, Universidad de Sevilla. Departamento de Medicina, Programa 'Juan Rodes' del Instituto de Salud Carlos III. Fondos FEDER, Programa 'Miguel Servet' del Instituto de Salud Carlos III. Fondos FEDER, Programa 'Nicolas Monardes' de la Consejería de Salud de la Junta de Andalucía, Programa Marco Horizonte 2020, and Ministerio de Educación, Cultura y Deporte (MECD). España

Abstract

Transcriptomics in Parkinson’s disease (PD) offers new insights into the molecular mechanism of PD pathogenesis. Several pathways, such as inflammation and protein degradation, have been identified by differential gene expression analysis. Our aim was to identify gene expression differences underlying the disease etiology and the discovery of pre-symptomatic risk biomarkers for PD from a multicenter study in the context of the PROPAG-AGEING project. We performed RNA sequencing from 47 patients with de novo PD, 10 centenarians, and 65 healthy controls. Using identified differentially expressed genes, functional annotations were assigned using gene ontology to unveil significant enriched biological processes. The expression of 16 selected genes was validated using OpenArray® assays and samples from independent cohorts of 201 patients with advanced PD, 340 healthy siblings of PD patients, and 177 healthy controls. Differential gene expression analysis identified higher FCGBP expression in patients with de novo PD compared with healthy controls and compared with centenarians. Furthermore, FCGBP showed no differences in terms of population origin or aging process. The increased FCGBP expression was validated in patients with advanced PD and their siblings. Thus, we provided evidence for an upregulation of FCGBP mRNA levels not only in patients with PD but also in individuals at putative higher risk of PD, suggesting that it could be important in gut–brain PD interaction, mediating the connection between microbiota and intestinal inflammatory processes, as well as neuroinflammation and neurodegeneration.

Published
2022

27. High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid

Author

Booiman, Thijs, Wit, Ferdinand W., Maurer, Irma, De Francesco, Davide, Sabin, Caroline A., Harskamp, Agnes M., Prins, Maria, Garagnani, Paolo, Pirazzini, Chiara, Franceschi, Claudio, Fuchs, Dietmar, Gisslén, Magnus, Winston, Alan, Reiss, Peter, Kootstra, Neeltje A., Reiss, P., Wit, F. W. N. M., Schouten, J., Kooij, K. W., van Zoest, R. A., Elsenga, B. C., Janssen, F. R., Heidenrijk, M., Zikkenheiner, W., van der Valk, M., Kootstra, N. A., Booiman, T., Harskamp-Holwerda, A. M., Boeser-Nunnink, B., Maurer, I., Mangas Ruiz, M. M., Girigorie, A. F., Villaudy, J., Frankin, E., Pasternak, A., Berkhout, B., van der Kuyl, T., Portegies, P., Schmand, B. A., Geurtsen, G. J., ter Stege, J. A., Klein Twennaar, M., Majoie, C. B. L. M., Caan, M. W. A., Su, T., Weijer, K., Bisschop, P. H. L. T., Kalsbeek, A., Wezel, M., Visser, I., Ruhé, H. G., Franceschi, C., Garagnani, P., Pirazzini, C., Capri, M., Dall’Olio, F., Chiricolo, M., Salvioli, S., Hoeijmakers, J., Pothof, J., Prins, M., Martens, M., Moll, S., Berkel, J., Totté, M., Kovalev, S., Gisslén, M., Fuchs, D., Zetterberg, H., Winston, A., Underwood, J., McDonald, L., Stott, M., Legg, K., Lovell, A., Erlwein, O., Doyle, N., Kingsley, C., Sharp, D. J., Leech, R., Cole, J. H., Zaheri, S., Hillebregt, M. M. J., Ruijs, Y. M. C., Benschop, D. P., Burger, D., de Graaff-Teulen, M., Guaraldi, G., Bürkle, A., Sindlinger, T., Moreno-Villanueva, M., Keller, A., Sabin, C., de Francesco, D., Libert, C., and Dewaele, S.

Published
2017
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28. Slug/β-Catenin–Dependent Proinflammatory Phenotype in Hypoxic Breast Cancer Stem Cells

Author

Storci, Gianluca, Bertoni, Sara, De Carolis, Sabrina, Papi, Alessio, Nati, Marina, Ceccarelli, Claudio, Pirazzini, Chiara, Garagnani, Paolo, Ferrarini, Alberto, Buson, Genny, Delledonne, Massimo, Fiorentino, Michelangelo, Capizzi, Elisa, Gruppioni, Elisa, Taffurelli, Mario, Santini, Donatella, Franceschi, Claudio, Bandini, Giuseppe, Bonifazi, Francesca, and Bonafé, Massimiliano

Published
2013
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29. DNA Methylation Analysis of Ribosomal DNA in Adults With Down Syndrome

Author

Ravaioli, Francesco, primary, Zampieri, Michele, additional, Morandi, Luca, additional, Pirazzini, Chiara, additional, Pellegrini, Camilla, additional, De Fanti, Sara, additional, Gensous, Noémie, additional, Pirazzoli, Gian Luca, additional, Sambati, Luisa, additional, Ghezzo, Alessandro, additional, Ciccarone, Fabio, additional, Reale, Anna, additional, Monti, Daniela, additional, Salvioli, Stefano, additional, Caiafa, Paola, additional, Capri, Miriam, additional, Bürkle, Alexander, additional, Moreno-Villanueva, Maria, additional, Garagnani, Paolo, additional, Franceschi, Claudio, additional, and Bacalini, Maria Giulia, additional

Published
2022
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31. Heterogeneity of prodromal Parkinson symptoms in siblings of Parkinson disease patients

Author

Baldelli, Luca, Schade, Sebastian, Jesús, Silvia, Schreglmann, Sebastian R., Sambati, Luisa, Gómez-Garre, Pilar, Halsband, Claire, Calandra-Buonaura, Giovanna, Adarmes-Gómez, Astrid Daniela, Sixel-Döring, Friederike, Zenesini, Corrado, Pirazzini, Chiara, Garagnani, Paolo, Bacalini, Maria Giulia, Bhatia, Kailash P., Cortelli, Pietro, Mollenhauer, Brit, Franceschi, Claudio, Mir, Pablo, Trenkwalder, Claudia, Provini, Federica, Houlden, Henry, Liò, Pietro, Luchinat, Claudio, Delledonne, Massimo, Mills, Kevin, Pedersen, Nancy L., Azevedo, Tiago, Bartoletti-Stella, Anna, Bonilla-Toribio, Marta, Buiza-Rueda, Dolores, Capellari, Sabina, Carriòn-Claro, Mario, Clayton, Robert, Dal Molin, Alessandra, Dimitri, Giovanna Maria, Doykov, Ivan, Giuliani, Cristina, Hägg, Sara, Hällqvist, Jenny, Heywood, Wendy, Huertas, Ismael, Jylhävä, Juulia, Labrador-Espinosa, Miguel A., Licari, Cristina, Macias, Daniel, Magrinelli, Francesca, Rodríguez, Juan Francisco Martín, Maturo, Maria Giovanna, Mengozzi, Giacomo, Meoni, Gaia, Milazzo, Maddalena, Nardini, Christine, Periñán-Tocino, Maria Teresa, Ravaioli, Francesco, Sala, Claudia, Spasov, Simeon, Tejera-Parrado, Cristina, Tenori, Leonardo, Paola, Turano, Williams, Dylan, Xumerle, Luciano, Zago, Elisa, Broli, Marcella, De Massis, Patrizia, Escuela-Martin, Rocio, Fabbri, Giovanni, Gabellini, Anna, Guaraldi, Pietro, Macrì, Stefania, Nassetti, Stefania Alessandra, Scaglione, Cesa Lorella Maria, Valzania, Franco, Rosaria, Cilea, Mignani, Francesco, Ortega, Rosario Vigo, Boninsegna, Claudia, De Luca, Silvia, Schade, Sebastian [0000-0002-6316-6804], Gómez-Garre, Pilar [0000-0002-0437-6182], Mir, Pablo [0000-0003-1656-302X], Provini, Federica [0000-0001-9063-2658], and Apollo - University of Cambridge Repository

Subjects
692/617/375/1718, 692/53/2423, article, 692/499
Abstract

A prodromal phase of Parkinson’s disease (PD) may precede motor manifestations by decades. PD patients’ siblings are at higher risk for PD, but the prevalence and distribution of prodromal symptoms are unknown. The study objectives were (1) to assess motor and non-motor features estimating prodromal PD probability in PD siblings recruited within the European PROPAG-AGEING project; (2) to compare motor and non-motor symptoms to the well-established DeNoPa cohort. 340 PD siblings from three sites (Bologna, Seville, Kassel/Goettingen) underwent clinical and neurological evaluations of PD markers. The German part of the cohort was compared with German de novo PD patients (dnPDs) and healthy controls (CTRs) from DeNoPa. Fifteen (4.4%) siblings presented with subtle signs of motor impairment, with MDS-UPDRS-III scores not clinically different from CTRs. Symptoms of orthostatic hypotension were present in 47 siblings (13.8%), no different to CTRs (p = 0.072). No differences were found for olfaction and overall cognition; German-siblings performed worse than CTRs in visuospatial-executive and language tasks. 3/147 siblings had video-polysomnography-confirmed REM sleep behavior disorder (RBD), none was positive on the RBD Screening Questionnaire. 173/300 siblings had

Published
2021

32. A geroscience approach for Parkinson’s disease: Conceptual framework and design of PROPAG-AGEING project

Author

Pirazzini, Chiara, primary, Azevedo, Tiago, additional, Baldelli, Luca, additional, Bartoletti-Stella, Anna, additional, Calandra-Buonaura, Giovanna, additional, Dal Molin, Alessandra, additional, Dimitri, Giovanna Maria, additional, Doykov, Ivan, additional, Gómez-Garre, Pilar, additional, Hägg, Sara, additional, Hällqvist, Jenny, additional, Halsband, Claire, additional, Heywood, Wendy, additional, Jesús, Silvia, additional, Jylhävä, Juulia, additional, Kwiatkowska, Katarzyna Malgorzata, additional, Labrador-Espinosa, Miguel A., additional, Licari, Cristina, additional, Maturo, Maria Giovanna, additional, Mengozzi, Giacomo, additional, Meoni, Gaia, additional, Milazzo, Maddalena, additional, Periñán-Tocino, Maria Teresa, additional, Ravaioli, Francesco, additional, Sala, Claudia, additional, Sambati, Luisa, additional, Schade, Sebastian, additional, Schreglmann, Sebastian, additional, Spasov, Simeon, additional, Tenori, Leonardo, additional, Williams, Dylan, additional, Xumerle, Luciano, additional, Zago, Elisa, additional, Bhatia, Kailash P., additional, Capellari, Sabina, additional, Cortelli, Pietro, additional, Garagnani, Paolo, additional, Houlden, Henry, additional, Liò, Pietro, additional, Luchinat, Claudio, additional, Delledonne, Massimo, additional, Mills, Kevin, additional, Mir, Pablo, additional, Mollenhauer, Brit, additional, Nardini, Christine, additional, Pedersen, Nancy L., additional, Provini, Federica, additional, Strom, Stephen, additional, Trenkwalder, Claudia, additional, Turano, Paola, additional, Bacalini, Maria Giulia, additional, Franceschi, Claudio, additional, Adarmes-Gómez, Astrid, additional, Bonilla-Toribio, Marta, additional, Boninsegna, Claudia, additional, Broli, Marcella, additional, Buiza-Rueda, Dolores, additional, Carrión-Claro, Mario, additional, Cilea, Rosalia, additional, Clayton, Robert, additional, Molin, Alessandra Dal, additional, De Luca, Silvia, additional, De Massis, Patrizia, additional, Escuela-Martin, Rocio, additional, Fabbri, Giovanni, additional, Gabellini, Anna, additional, Giuliani, Cristina, additional, Guaraldi, Pietro, additional, Huertas, Ismae, additional, Macias, Daniel, additional, Macrì, Stefania, additional, Magrinelli, Francesca, additional, Rodríguez, Juan Francisco Martín, additional, Mignani, Francesco, additional, Nassetti, Stefania Alessandra, additional, Pirazzini, Chiara, additional, Scaglione, Cesa Lorella Maria, additional, Tejera-Parrado, Cristina, additional, Valzania, Franco, additional, and Ortega, Rosario Vigo, additional

Published
2021
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34. Do people living with HIV experience greater age advancement than their HIV-negative counterparts?

Author

De Francesco, Davide, Wit, Ferdinand W., Burkle, Alexander, Oehlke, Sebastian, Kootstra, Neeltje A., Winston, Alan, Franceschi, Claudio, Garagnani, Paolo, Pirazzini, Chiara, Libert, Claude, Grune, Tilman, Weber, Daniela, Jansen, Eugene H. J. M., Sabin, Caroline A., Reiss, Peter, Reiss, P., Winston, A., Wit, F. W., Prins, M., van der Loeff, M. F. Schim, Schouten, J., Schmand, B., Geurtsen, G. J., Sharp, D. J., Caan, M. W. A., Majoie, C., Villaudy, J., Berkhout, B., Kootstra, N. A., Gisslen, M., Pasternak, A., Sabin, C. A., Guaraldi, G., Burkle, A., Libert, C., Franceschi, C., Kalsbeek, A., Fliers, E., Hoeijmakers, J., Pothof, J., van der Valk, M., Bisschop, P. H., Portegies, P., Zaheri, S., Burger, D., Cole, J. H., Biirkle, A., Zikkenheiner, W., Janssen, F. R., Underwood, J., Kooij, K. W., van Zoest, R. A., Doyle, N., van der Loeff, M. Schim, Schmand, B. A., Verheij, E., Verboeket, S. O., Elsenga, B. C., Hillebregt, M. M. J., Ruijs, Y. M. C., Benschop, D. P., Tembo, L., McDonald, L., Stott, M., Legg, K., Lovell, A., Erlwein, O., Kingsley, C., Norsworthy, P., Mullaney, S., Kruijer, T., del Grande, L., Olthof, V, Visser, G. R., May, L., Verbraak, F., Demirkaya, N., Visser, I, Majoie, C. B. L. M., Su, T., Leech, R., Huguet, J., Frankin, E., van der Kuyl, A., Weijer, K., Siteur-Van Rijnstra, E., Harskamp-Holwerda, A. M., Maurer, I, Ruiz, M. M. Mangas, Girigorie, A. F., Boeser-Nunnink, B., Kals-Beek, A., Bisschop, P. H. L. T., de Graaff-Teulen, M., Dewaele, S., Garagnani, P., Pirazzini, C., Capri, M., Dall'Olio, F., Chiricolo, M., Salvioli, S., Fuchs, D., Zetterberg, H., Weber, D., Grune, T., Jansen, E. H. J. M., De Francesco, D., Sindlinger, T., Oehlke, S., Global Health, AII - Infectious diseases, APH - Aging & Later Life, Experimental Immunology, ANS - Neurodegeneration, AMS - Restoration & Development, Medical Psychology, and APH - Mental Health

Subjects
Male, 0301 basic medicine, CYTOMEGALOVIRUS, HIV Infections, DISEASE, 0302 clinical medicine, Biomarkers of aging, Medicine and Health Sciences, Immunology and Allergy, 030212 general & internal medicine, the Co-morBidity in Relation to AIDS (COBRA) Collaboration, POPULATION, Immunodeficiency, education.field_of_study, premature aging, virus diseases, 11 Medical And Health Sciences, Middle Aged, Hepatitis B, SOUTH-AFRICA, Infectious Diseases, Anti-Retroviral Agents, Cohort, Female, Life Sciences & Biomedicine, medicine.drug, Adult, Premature aging, medicine.medical_specialty, BIOMARKERS, Immunology, Population, biomarkers of aging, 17 Psychology And Cognitive Sciences, 03 medical and health sciences, Acquired immunodeficiency syndrome (AIDS), Virology, ddc:570, Internal medicine, medicine, Humans, accelerated aging, education, Aged, accelerated aging, aging, biological age, biomarkers of aging, HIV, premature aging, Science & Technology, business.industry, aging, Biology and Life Sciences, HIV, 06 Biological Sciences, medicine.disease, COMORBIDITIES, biological age, INFECTED INDIVIDUALS, IMMUNOGLOBULIN-G ANTIBODY, PROTEASE INHIBITORS, Cross-Sectional Studies, 030104 developmental biology, RISK-FACTORS, business, Saquinavir
Abstract

Objectives: Despite successful antiretroviral (ARV) therapy, people living with HIV (PLWH) may show signs of premature/accentuated aging. We compared established biomarkers of aging in PLWH, appropriately-chosen HIV-negative individuals, and blood donors, and explored factors associated with biological age advancement.Design: Cross-sectional analysis of 134 PLWH on suppressive ARV therapy, 79 lifestyle-comparable HIV-negative controls aged ≥45 years from the Co-morBidity in Relation to AIDS (COBRA) cohort, and 35 age-matched blood donors (BD).Methods: Biological age was estimated using a validated algorithm based on ten biomarkers. Associations between ‘age advancement’ (biological minus chronological age) and HIV status/parameters, lifestyle, cytomegalovirus (CMV), hepatitis B (HBV) and hepatitis C virus (HCV) infections were investigated using linear regression.Results: The average (95% CI) age advancement was greater in both HIV-positive [13.2 (11.6, 14.9) years] and HIV-negative [5.5 (3.8, 7.2) years] COBRA participants compared to BD [-7.0 (-4.1, -9.9) years, both p's < 0.001)], but also in HIV-positive compared to HIV-negative participants (p < 0.001). Chronic HBV, higher anti-CMV IgG titer and CD8+ T-cell count were each associated with increased age advancement, independently of HIV-status/group. Among HIV-positive participants, age advancement was increased by 3.5 (0.1, 6.8) years among those with nadir CD4+ < 200 cells/μL and by 0.1 (0.06, 0.2) years for each additional month of exposure to saquinavir.Conclusions: Both treated PLWH and lifestyle-comparable HIV-negative individuals show signs of age advancement compared to BD, to which persistent CMV, HBV co-infection and CD8+ T-cell activation may have contributed. Age advancement remained greatest in PLWH and was related to prior immunodeficiency and cumulative saquinavir exposure. published

Published
2019

35. New DNA Methylation Signals for Malignant Pleural Mesothelioma Risk Assessment

Author

Cugliari, Giovanni, primary, Allione, Alessandra, additional, Russo, Alessia, additional, Catalano, Chiara, additional, Casalone, Elisabetta, additional, Guarrera, Simonetta, additional, Grosso, Federica, additional, Ferrante, Daniela, additional, Sculco, Marika, additional, La Vecchia, Marta, additional, Pirazzini, Chiara, additional, Libener, Roberta, additional, Mirabelli, Dario, additional, Magnani, Corrado, additional, Dianzani, Irma, additional, and Matullo, Giuseppe, additional

Published
2021
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36. Whole-genome sequencing analysis of semi-supercentenarians

Author

Garagnani, Paolo, primary, Marquis, Julien, additional, Delledonne, Massimo, additional, Pirazzini, Chiara, additional, Marasco, Elena, additional, Kwiatkowska, Katarzyna Malgorzata, additional, Iannuzzi, Vincenzo, additional, Bacalini, Maria Giulia, additional, Valsesia, Armand, additional, Carayol, Jerome, additional, Raymond, Frederic, additional, Ferrarini, Alberto, additional, Xumerle, Luciano, additional, Collino, Sebastiano, additional, Mari, Daniela, additional, Arosio, Beatrice, additional, Casati, Martina, additional, Ferri, Evelyn, additional, Monti, Daniela, additional, Nacmias, Benedetta, additional, Sorbi, Sandro, additional, Luiselli, Donata, additional, Pettener, Davide, additional, Castellani, Gastone, additional, Sala, Claudia, additional, Passarino, Giuseppe, additional, De Rango, Francesco, additional, D'Aquila, Patrizia, additional, Bertamini, Luca, additional, Martinelli, Nicola, additional, Girelli, Domenico, additional, Olivieri, Oliviero, additional, Giuliani, Cristina, additional, Descombes, Patrick, additional, and Franceschi, Claudio, additional

Published
2021
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37. A Meta-Analysis of Brain DNA Methylation Across Sex, Age, and Alzheimer's Disease Points for Accelerated Epigenetic Aging in Neurodegeneration

Author

Pellegrini, Camilla, primary, Pirazzini, Chiara, additional, Sala, Claudia, additional, Sambati, Luisa, additional, Yusipov, Igor, additional, Kalyakulina, Alena, additional, Ravaioli, Francesco, additional, Kwiatkowska, Katarzyna M., additional, Durso, Danielle F., additional, Ivanchenko, Mikhail, additional, Monti, Daniela, additional, Lodi, Raffaele, additional, Franceschi, Claudio, additional, Cortelli, Pietro, additional, Garagnani, Paolo, additional, and Bacalini, Maria Giulia, additional

Published
2021
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38. Author response: Whole-genome sequencing analysis of semi-supercentenarians

Author

Garagnani, Paolo, primary, Marquis, Julien, additional, Delledonne, Massimo, additional, Pirazzini, Chiara, additional, Marasco, Elena, additional, Kwiatkowska, Katarzyna Malgorzata, additional, Iannuzzi, Vincenzo, additional, Bacalini, Maria Giulia, additional, Valsesia, Armand, additional, Carayol, Jerome, additional, Raymond, Frederic, additional, Ferrarini, Alberto, additional, Xumerle, Luciano, additional, Collino, Sebastiano, additional, Mari, Daniela, additional, Arosio, Beatrice, additional, Casati, Martina, additional, Ferri, Evelyn, additional, Monti, Daniela, additional, Nacmias, Benedetta, additional, Sorbi, Sandro, additional, Luiselli, Donata, additional, Pettener, Davide, additional, Castellani, Gastone, additional, Sala, Claudia, additional, Passarino, Giuseppe, additional, De Rango, Francesco, additional, D'Aquila, Patrizia, additional, Bertamini, Luca, additional, Martinelli, Nicola, additional, Girelli, Domenico, additional, Olivieri, Oliviero, additional, Giuliani, Cristina, additional, Descombes, Patrick, additional, and Franceschi, Claudio, additional

Published
2021
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39. Insights From Liver-Humanized Mice on Cholesterol Lipoprotein Metabolism and LXR-Agonist Pharmacodynamics in Humans

Author

Minniti, Mirko E., Pedrelli, Matteo, Vedin, Lise-Lotte, Delbes, Anne-Sophie, Denis, Raphael G. P., Öörni, Katariina, Sala, Claudia, Pirazzini, Chiara, Thiagarajan, Divya, Nurmi, Harri J., Grompe, Markus, Mills, Kevin, Garagnani, Paolo, Ellis, Ewa C. S., Strom, Stephen C., Luquet, Serge H., Wilson, Elizabeth M., Bial, John, Steffensen, Knut R., Parini, Paolo, Kari Alitalo / Principal Investigator, CAN-PRO - Translational Cancer Medicine Program, University of Helsinki, Research Programs Unit, and HUS Helsinki and Uusimaa Hospital District

Subjects
HUMAN HEPATOCYTES, ENGRAFTMENT, PLASMA, ATHEROSCLEROSIS, X-RECEPTOR AGONIST, 3121 General medicine, internal medicine and other clinical medicine, CETP, APOLIPOPROTEINS, lipids (amino acids, peptides, and proteins), PHOSPHOLIPID TRANSFER PROTEIN, MOUSE, LDL
Abstract

Background and Aims Genetically modified mice have been used extensively to study human disease. However, the data gained are not always translatable to humans because of major species differences. Liver-humanized mice (LHM) are considered a promising model to study human hepatic and systemic metabolism. Therefore, we aimed to further explore their lipoprotein metabolism and to characterize key hepatic species-related, physiological differences. Approach and Results Fah(-/-), Rag2(-/-), and Il2rg(-/-) knockout mice on the nonobese diabetic (FRGN) background were repopulated with primary human hepatocytes from different donors. Cholesterol lipoprotein profiles of LHM showed a human-like pattern, characterized by a high ratio of low-density lipoprotein to high-density lipoprotein, and dependency on the human donor. This pattern was determined by a higher level of apolipoprotein B100 in circulation, as a result of lower hepatic mRNA editing and low-density lipoprotein receptor expression, and higher levels of circulating proprotein convertase subtilisin/kexin type 9. As a consequence, LHM lipoproteins bind to human aortic proteoglycans in a pattern similar to human lipoproteins. Unexpectedly, cholesteryl ester transfer protein was not required to determine the human-like cholesterol lipoprotein profile. Moreover, LHM treated with GW3965 mimicked the negative lipid outcomes of the first human trial of liver X receptor stimulation (i.e., a dramatic increase of cholesterol and triglycerides in circulation). Innovatively, LHM allowed the characterization of these effects at a molecular level. Conclusions LHM represent an interesting translatable model of human hepatic and lipoprotein metabolism. Because several metabolic parameters displayed donor dependency, LHM may also be used in studies for personalized medicine.

Published
2020

40. Complex sex-specific age-related changes in DNA methylation including variability, epimutations and entropy

Author

Yusipov, Igor, Bacalini, Maria Giulia, Kalyakulina, Alena, Krivonosov, Mikhail, Pirazzini, Chiara, Gensous, Noémie, Ravaioli, Francesco, Milazzo, Maddalena, Vedunova, Maria, Fiorito, Giovanni, Gagliardi, Amedeo, Polidoro, Silvia, Garagnani, Paolo, Ivanchenko, Mikhail, and Franceschi, Claudio

Abstract

Current epidemiological data indicate that, in humans, females live longer than males but experience a worse quality of life in advanced age. The reasons for this sex disparity are still unknown, but it is likely that it derives from a strict interplay between biological and cultural factors. Epigenetic modifications likely contribute to shape sex gap in aging and longevity, and genome-wide DNA methylation differences between males and females in autosomal chromosomes have been reported. Several studies showed that DNA methylation patterns are profoundly remodelled during aging, modulated in part by environmental exposures. However, few studies have specifically investigated if DNA methylation is differently affected by aging in males and females. Here we performed a meta-analysis of 4 large whole blood datasets including males and females of different ages and we compared 4 aspects of epigenetic age-dependent remodelling between males and females: normative changes, variability, epimutations, and entropy. While we did not find differences in the age-associated increase in epimutations and in entropy, we reported a list of highly reproducible sex-specific age-associated differentially methylated positions (saDMPs) and sex-specific age-associated variably methylated positions (saVMPs). We investigated the enrichment in saDMPs and saVMPs in genomic regions, imprinted and sex hormone-related genes and Reactome pathways. Furthermore, we experimentally validated the most robust saDMPs, mapping in FIGN and PRR4 genes, and showed sex-specific deviations of their methylation patterns in models of successful (centenarians) and unsuccessful (Down syndrome) aging. In conclusion, we provided a comprehensive description of sex-differences in DNA methylation changes with aging in whole blood. Our results can pave the way to the identification of possible molecular triggers of the sex gap in aging and longevity.

Published
2020
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41. New insights from liver‐humanized mice on cholesterol lipoprotein metabolism and LXR‐agonist pharmacodynamics in humans

Author

Minniti, Mirko, Pedrelli, Matteo, Vedin, Lise‐Lotte, Delbès, Anne‐Sophie, Denis, Raphaël G.P., Öörni, Katariina, Sala, Claudia, Pirazzini, Chiara, Thiagarajan, Divya, Nurmi, Harri, Grompe, Markus, Mills, Kevin, Garagnani, Paolo, Ellis, Ewa C.S., Strom, Stephen, Luquet, Serge, Wilson, Elizabeth, Bial, John, Steffensen, Knut, Parini, Paolo, Department of Laboratory Medicine [Karolinska Institutet], Karolinska Institutet [Stockholm], University of Helsinki, Physics Laboratory (NIST), National Institute of Standards and Technology [Gaithersburg] (NIST), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Karolinska Institut, and Karolinska University Hospital [Stockholm]

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2019

42. Age-related DNA methylation changes are sex-specific: a comprehensive assessment

Author

Yusipov, Igor, primary, Bacalini, Maria Giulia, additional, Kalyakulina, Alena, additional, Krivonosov, Mikhail, additional, Pirazzini, Chiara, additional, Gensous, Noémie, additional, Ravaioli, Francesco, additional, Milazzo, Maddalena, additional, Giuliani, Cristina, additional, Vedunova, Maria, additional, Fiorito, Giovanni, additional, Gagliardi, Amedeo, additional, Polidoro, Silvia, additional, Garagnani, Paolo, additional, Ivanchenko, Mikhail, additional, and Franceschi, Claudio, additional

Published
2020
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43. DNA Methylation of FKBP5 as Predictor of Overall Survival in Malignant Pleural Mesothelioma

Author

Cugliari, Giovanni, primary, Catalano, Chiara, additional, Guarrera, Simonetta, additional, Allione, Alessandra, additional, Casalone, Elisabetta, additional, Russo, Alessia, additional, Grosso, Federica, additional, Ferrante, Daniela, additional, Viberti, Clara, additional, Aspesi, Anna, additional, Sculco, Marika, additional, Pirazzini, Chiara, additional, Libener, Roberta, additional, Mirabelli, Dario, additional, Magnani, Corrado, additional, Dianzani, Irma, additional, and Matullo, Giuseppe, additional

Published
2020
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44. Methylation of ELOVL2 gene as a new epigenetic marker of age

Author

Garagnani, Paolo, Bacalini, Maria G., Pirazzini, Chiara, Gori, Davide, Giuliani, Cristina, Mari, Daniela, Di Blasio, Anna M., Gentilini, Davide, Vitale, Giovanni, Collino, Sebastiano, Rezzi, Serge, Castellani, Gastone, Capri, Miriam, Salvioli, Stefano, and Franceschi, Claudio

Published
2012
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45. Analysis of Epigenetic Age Predictors in Pain-Related Conditions

Author

Kwiatkowska, Katarzyna Malgorzata, primary, Bacalini, Maria Giulia, additional, Sala, Claudia, additional, Kaziyama, Helena, additional, de Andrade, Daniel Ciampi, additional, Terlizzi, Rossana, additional, Giannini, Giulia, additional, Cevoli, Sabina, additional, Pierangeli, Giulia, additional, Cortelli, Pietro, additional, Garagnani, Paolo, additional, and Pirazzini, Chiara, additional

Published
2020
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46. Insights From Liver‐Humanized Mice on Cholesterol Lipoprotein Metabolism and LXR‐Agonist Pharmacodynamics in Humans

Author

Minniti, Mirko E., primary, Pedrelli, Matteo, additional, Vedin, Lise‐Lotte, additional, Delbès, Anne‐Sophie, additional, Denis, Raphaël G.P., additional, Öörni, Katariina, additional, Sala, Claudia, additional, Pirazzini, Chiara, additional, Thiagarajan, Divya, additional, Nurmi, Harri J., additional, Grompe, Markus, additional, Mills, Kevin, additional, Garagnani, Paolo, additional, Ellis, Ewa C.S., additional, Strom, Stephen C., additional, Luquet, Serge H., additional, Wilson, Elizabeth M., additional, Bial, John, additional, Steffensen, Knut R., additional, and Parini, Paolo, additional

Published
2020
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47. Aging and Caloric Restriction Modulate the DNA Methylation Profile of the Ribosomal RNA Locus in Human and Rat Liver

Author

Gensous, Noémie, primary, Ravaioli, Francesco, additional, Pirazzini, Chiara, additional, Gramignoli, Roberto, additional, Ellis, Ewa, additional, Storci, Gianluca, additional, Capri, Miriam, additional, Strom, Stephen, additional, Laconi, Ezio, additional, Franceschi, Claudio, additional, Garagnani, Paolo, additional, Marongiu, Fabio, additional, and Bacalini, Maria Giulia, additional

Published
2020
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48. MOESM1 of Regulatory T cells from patients with end-stage organ disease can be isolated, expanded and cryopreserved according good manufacturing practice improving their function

Author

Ulbar, Francesca, Montemurro, Tiziana, Jofra, Tatiana, Capri, Miriam, Comai, Giorgia, Bertuzzo, Valentina, Lavazza, Cristiana, Mandelli, Alessandra, Viganò, Mariele, Budelli, Silvia, Bacalini, Maria, Pirazzini, Chiara, Garagnani, Paolo, Giudice, Valeria, Sollazzo, Daria, Curti, Antonio, Arpinati, Mario, Manna, Gaetano La, Cescon, Matteo, Pinna, Antonio, Franceschi, Claudio, Battaglia, Manuela, Giordano, Rosaria, Catani, Lucia, and Lemoli, Roberto

Subjects
chemical and pharmacologic phenomena
Abstract

Additional file 1: Table S1. Antibodies for flow cytometry. Figure S1. Design of the in vivo study. A. Steady state leukapheresis from 2 patients (1 KT and 1 LT patient) were processed using GMP-compliant devices and reagents. Tregs positive fraction (CD8−CD25+ cells) was purified using the CliniMACS System. Forty millions of CD8−CD25 + cells were expanded in vitro for 3 weeks. At day 21 all the cultured cells were collected and the beads were removed using the CliniMACS device, according to manufacturer’s instructions. Negative fraction (CD8−CD25− T cells) after GMP selection at day 0 and the final product after GMP expansion at day 21 were cryopreserved and thawed as described in “Materials and methods” section. B. Irradiated NSG mice were infused with the KT or the LT CD8−CD25− T cells, either alone or in combination with autologous expanded Tregs at 1:1 ratio, to assess their ability to ameliorate GVHD. C. Mice were bled 4/7 weeks after transplantation and sacrificed 7 weeks after transplantation. FACS analysis of the injected cells (day 1), of PB (4 weeks ± 3 days after transplantation) and of PB and spleen (7 weeks ± 3 days after transplantation) was performed. Figure S2. Circulating Tregs in KT and LT patients. Mean absolute number of circulating CD4+CD25+CD127−FoxP3+ Tregs from healthy controls and selected LT and KT patients (p = NS).

Published
2019
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49. Genomics and epigenomics

Author

Garagnani, Paolo, Terlizzi, Rossana, Cevoli, Sabina, Capellari, Sabina, Pierangeli, Giulia, Pirazzini, Chiara, Bacalini, Maria Giulia, Franceschi, Claudio, and Cortelli, Pietro

Published
2015
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50. Effect of Extremely Low-Frequency Pulsed Magnetic Field Exposure on DNA methylation

Author

GIORGI, GIANFRANCO, PIRAZZINI, CHIARA, BACALINI, MARIA GIULIA, GIULIANI, CRISTINA, GARAGNANI, PAOLO, CAPRI, MIRIAM, BERSANI, FERDINANDO, DEL RE, BRUNELLA, Salvatore Caorsi, Paolo Ravazzani, Marta Pirazzini, Giorgi, Gianfranco, Pirazzini, Chiara, Bacalini, Maria Giulia, Giuliani, Cristina, Garagnani, Paolo, Capri, Miriam, Bersani, Ferdinando, and Del Re, Brunella

Subjects
Extremely low frequency magnetic field, retrotransposition, LINE-1, epigenetics, DNA methylation
Abstract

We investigated the question of whether exposure to extremely low frequency pulsed magnetic fields (PMF) can affect DNA methylation levels. We analyzed the methylation levels of 5’untraslated region (5’UTR) of long interspersed nuclear element-1s in BE(2)C cells exposed to PMF or sham. Results show that PMF induces changes of DNA methylation, but these are not probably biologically relevant.

Published
2016

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