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The use of arsenic (As) tolerant plants and those with a well-developed root system have shown great relevance in phytoremediation campaigns. Corymbia citriodora presents these characteristics that can be enhanced by humic substances (HS) from organic matter. The objective of the present study was to determine if HS extracted from vermicompost can modify the root system architecture, stimulate growth, increase robustness and relieve stress promoted by As in C. citriodora seedlings. Two preliminary tests were carried out to obtain the best concentration-response for HS and the toxic As concentration. Subsequently, an experiment was carried out in nursery pots with nutrient solution and four treatments: Control, HS, As and As + HS. HS alleviated the reduced growth caused by As with regards to dry mass, root collar diameter and height of the aerial part. The Dickson quality index (DQI) was reduced by the action of As and this negative affect was mitigated by HS. Our results show the positive effect of the HS in alleviating As abiotic stress in C. citriodora seedlings, demonstrating its potential to mitigate the toxicity of this chemical element. [ABSTRACT FROM AUTHOR]

Effects of traditional salt placement ( TS) and strategically placed mineral mix supplements ( MMS) on cattle distribution were compared in the Italian Alps. Salt was placed within flat and herbaceous sites following the traditional method of farmers, whereas MMS blocks were placed on steep and shrub-encroached sites within three large pastures (44 ha on average). Each TS and MMS site was paired with a control site, and eleven cows were tracked with GPS collars. Within 10 and 50 m of TS and MMS, treatment sites were used more than control sites ( P < 0·05). No differences were detected ( P > 0·05) in the number of visits by cows within 10 and 50 m of TS and MMS placements. No differences were detected in time spent within 10 m of TS and MMS placements, but cows spent more time ( P < 0·05) within 50 m of TS. With either TS or MMS, cattle preferred areas with gentler terrain, higher forage pastoral value and closer to water ( P < 0·001). When MMS was available cattle also preferred areas near MMS ( P < 0·001). Shrub and herbaceous cover decreased near MMS placements ( P < 0·05) from cattle grazing and trampling. Placement of MMS is a promising tool to enhance cattle distribution and potentially reduce shrub encroachment in rugged mountain pastures. [ABSTRACT FROM AUTHOR]

Pediatric high-grade glioma (pHGG) encompasses a wide range of gliomas with different genomic, epigenomic, and transcriptomic features. Almost 50% of pHGGs present a mutation in genes coding for histone 3, including the subtype harboring the H3.3-G34 mutation. In this context, histone mutations are frequently associated with mutations in TP53 and ATRX , along with PDGFRA and NOTCH2NL amplifications. Moreover, the H3.3-G34 histone mutation induces epigenetic changes in immune-related genes and exerts modulatory functions on the microenvironment. Also, the functionality of the blood-brain barrier (BBB) has an impact on treatment response. The prognosis remains poor with conventional treatments, thus eliciting the investigation of additional and alternative therapies. Promising molecular targets include PDGFRA amplification, BRAF mutation, EGFR amplification, NF1 loss, and IDH mutation. Considering that pHGGs harboring the H3.3-G34R mutation appear to be more susceptible to immunotherapies (ITs), different options have been recently explored, including immune checkpoint inhibitors, antibody mediated IT, and Car-T cells. This review aims to summarize the knowledge concerning cancer biology and cancer-immune cell interaction in this set of pediatric gliomas, with a focus on possible therapeutic options.

Background: Technological advancements have been rapidly integrated within the neurosurgical education track since it is a high-risk specialty with little margin for error. Indeed, simulation and virtual reality during training can improve surgical performance and technical skills. Our study aims to investigate the impact of neurosurgical technology-based simulation activities on medical students., Methods and Materials: The "Suturing Mission-The Symposium" was a three-day event held at Humanitas University. Participants had access to live-streamed conferences held by worldwide experts in several fields of neurosurgery and practical simulations of dura mater sutures, microvascular anastomosis, and augmented reality neurosurgical approaches. An anonymous survey was conducted at the beginning and end of the event., Results: 141 medical students with a mean age of 21 participated. After the course, 110 participants (77.5%) showed interest in pursuing a surgical path, with a great prevalence in those who had planned to have a surgical career before the event (88.7% vs. 41.4%, p < 0.001). Participants were also asked about their comfort levels while handling surgical instruments, and a good outcome was reached in 72.7% of participants, with a significant difference between those who had previously attended a suture course (87.8% vs. 66.3%, p = 0.012)., Conclusion: Training sessions on surgical simulators were effective in increasing participants' interest in pursuing a surgical path, improving their understanding of postgraduate orientation, and boosting their confidence with surgical instruments.

Knowledge of how grazing cattle utilize heterogeneous landscapes in Mediterranean silvopastoral areas is scarce. Global positioning systems (GPS) to track animals, together with geographic information systems (GIS), can relate animal distribution to landscape features. With the aim to develop a general spatial model that provides accurate prediction of cattle resource selection patterns within a Mediterranean mountainous silvopastoral area, free-roaming Sarda cows were fitted with GPS collars to track their spatial behaviors. Resource selection function models (RSF) were developed to estimate the probability of resource use as a function of environmental variables. A set of over 500 candidate RSF models, composed of up to five environmental predictor variables, were fitted to data. To identify a final model providing a robust prediction of cattle resource selection pattern across the different seasons, the 10 best models (ranked on the basis of the AIC score) were fitted to seasonal data. Prediction performance of the models was evaluated with a Spearman correlation analysis using the GPS position data sets previously reserved for model validation. The final model emphasized that watering point, elevation, and distance to fences were important factors affecting cattle resource-selection patterns. The prediction performances (as Spearman rank correlation scores) of the final model, when fitted to each season, ranged between 0.7 and 0.94. The cows were more likely to select areas lower in elevation and farther from the watering point in winter than in summer (693 ± 1 m and 847 ± 13 m vs. 707 ± 1 m and 635 ± 21 m, respectively), and in spring opted for the areas furthest from the water (963 ± 12). Although caution should be exercised in generalizing to other silvopastoral areas, the satisfactory Spearman correlations scores from the final RSF model applied to different seasons indicate resource selection function is a powerful predictive model. The relative importance of the individual predictors within the model varied among the different seasons, demonstrating the RSF model's ability to interpret changes in animal behavior at different times of the year. The RSF model has proven to be a useful tool to interpret the spatial behaviors of cows grazing in Mediterranean silvopastoral areas and could therefore be helpful in managing and preserving ecosystem services of these areas., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer MB declared a shared parent affiliation with the author PC to the handling editor at the time of review., (Copyright © 2024 Acciaro, Pittarello, Decandia, Sitzia, Giovanetti, Lombardi and Clark.)

Gliomas are diffusely infiltrating brain tumors whose prognosis is strongly influenced by their extent of invasion into the surrounding brain tissue. While lower-grade gliomas present more circumscribed borders, high-grade gliomas are aggressive tumors with widespread brain infiltration and dissemination. Glioblastoma (GBM) is known for its high invasiveness and association with poor prognosis. Its low survival rate is due to the certainty of its recurrence, caused by microscopic brain infiltration which makes surgical eradication unattainable. New insights into GBM biology at the single-cell level have enabled the identification of mechanisms exploited by glioma cells for brain invasion. In this review, we explore the current understanding of several molecular pathways and mechanisms used by tumor cells to invade normal brain tissue. We address the intrinsic biological drivers of tumor cell invasion, by tackling how tumor cells interact with each other and with the tumor microenvironment (TME). We focus on the recently discovered neuronal niche in the TME, including local as well as distant neurons, contributing to glioma growth and invasion. We then address the mechanisms of invasion promoted by astrocytes and immune cells. Finally, we review the current literature on the therapeutic targeting of the molecular mechanisms of invasion.

Trees and shrubs expanded in the last decades in European mountains due to land abandonment and the decrease in grazing pressure, and are expected to further spread also due to climate change. As a consequence of low forage quality and topographic constraints, the management of mountain environments dominated by woody vegetation with livestock is often challenging. Silvopastoral systems based on cattle hardy breeds able to forage on woody plants, such as Highland cattle, could be a suitable option for the management and restoration of such environments. In this study, we used direct observations to explore the foraging behavior of Highland cattle in four study areas across the western Alps. In particular, we assessed: (1) cattle diet composition, (2) the selection for more than 30 tree and shrub species, and (3) the relationships between species consumption and their abundance in the environment. Highland cattle fed on a mixture of both woody and herbaceous species, including between 15 and 46% of woody plants in the diet. Some trees (e.g., Celtis australis , Fraxinus spp., and Populus tremula ) and shrubs (e.g., Frangula alnus , Rhamnus spp., and Rubus idaeus ) were positively selected by cattle, thus they could be an important forage supplement to their diet. Moreover, the results highlighted that relative species consumption generally increased with increasing species abundance in the environment, suggesting that this cattle breed could be suitable to control shrub expansion in highly encroached areas. The outcomes of this study can support the development of targeted silvopastoral systems in the Alps., Supplementary Information: The online version contains supplementary material available at 10.1007/s10457-023-00926-z., Competing Interests: Competing interestsThe authors declare no competing interests.Conflict of interestCorresponding author, on behalf of all the co-authors, discloses any financial and personal relationships with other people or organizations that could inappropriately influence the work., (© The Author(s) 2023.)

Objective: Blue light (410-430nm) has been suggested to be effective in the healing process of hard-to-heal wounds. The aim of this study was to test this hypothesis., Method: This single-centre observational study assessed the efficacy of photobiomodulation with blue light (120 seconds at a distance of 4cm from the wound bed once a week for four weeks) in activating healing in patients with hard-to-heal skin lesions (mean duration 23.9 months) of the lower limb that had not responded to four weeks of standard treatment., Results: A total of 59 patients were assessed. Wounds were divided into groups according to aetiology: hard-to-heal venous wound (30.5%); hard-to-heal arterial and mixed wounds (16.9%); hard-to-heal inflammatory wound (22.0%); other hard-to-heal wounds (13.6%); and acute wound (16.9%). The mean reduction in wound area at the end of the four-week treatment period with blue light compared with baseline was 51.38% (p<0.001) across all wounds. Among subgroups, the best performance was obtained for hard-to-heal venous wounds, achieving a 63.36% (p<0.001) mean reduction in wound size, and acute wounds, achieving a mean reduction of 82.76% (p<0.001). The greatest mean change in wound bed score was seen at the end of the four-week treatment period with blue light, with an increase in the mean score from 8.6 at baseline to 12.8 at week four (77.2%; p<0.001). There was a clear decrease in pain during blue light treatment, achieving a reduction in the average numeric rating scale (NRS) from 4.3 at baseline to 1.8 at week 4 (53.23%; p <0.001). At week 4, the highest proportion of complete healing was seen in acute (100.0%) and venous wounds (83.3%)., Conclusion: Based on these results, photobiomodulation with blue light appears to reactivate the healing process in acute and hard-to-heal wounds that do not respond to standard treatment. Photobiomodulation with blue light treatment is easy to perform and safe, with no reported adverse events or side-effects., Competing Interests: Declaration of interest: The authors have no conflicts of interest to declare.

Background: In order to improve the biological control agent (BCA) efficacy, stress factors threatening the viability of microorganisms during spray application need to be determined. The effect of spray mixture temperature and exposure time on Trichoderma harzianum T 22 and Bacillus amyloliquefaciens QST713 viability were tested. Concurrently the combined effect of mechanical and thermal stress effect on BCA viability were tested at two initial spray mixture temperatures (14 and 25 °C) by simulating a spray application using airblast sprayers featured by different tank capacity and a spray liquid circuit (without and with hydraulic agitation system). To assess the BCA microorganism viability, spray mixture samples were collected at time intervals along trials and plated to count the colony forming units (CFU)., Results: The critical temperature threshold that inhibited BCA viability was 35 °C with 30 min of exposure. The sprayer type, the initial temperature of the spray mixture and the temperature increment during the trials significantly decreased the number of CFU recovered. When simulating a spray application, the spray mixture temperature increase rate was determined mainly by the residual amount of spray mixture in the tank. Even if the tank capacity does not substantially affect the final temperature reached by the spray mixture, the higher residual spray mixture in bigger tanks can expose the BCAs for a longer time to critical temperatures., Conclusions: Experimental trials allowed us to identify the effect of factors affecting the viability of tested BCAs, providing information about the actual chance to guarantee the biological efficacy of BCA treatments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry., (© 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)

During the last decades, Alnus viridis has expanded over former montane pastures and meadows, due to land use and abandonment. This nitrogen-fixing woody species has triggered negative agro-environmental impacts, such as nitrogen (N) leaching, soil acidification and a reduced biodiversity. The aim of this study was to estimate the N translocation from A. viridis -encroached areas to adjacent open pastures by Highland cattle. In 2019 and 2020, Highland cattle herds equipped with GPS collars were placed in four A. viridis -encroached paddocks across Italy and Switzerland. The N content was measured in A. viridis leaves, herbaceous vegetation, and cattle dung pats, which were collected throughout the grazing season. Using GPS locations and collar activity sensors, livestock activity phases were discriminated. The N ingested by cattle was estimated through the N content of herbaceous vegetation and A. viridis leaves of vegetation patches visited by cattle during 24 h before dung sampling (N 24H ). The N content of herbaceous vegetation significantly increased with increasing A. viridis cover. The average N content in dung pats (31.2 ± 3.4 g.kg -1 DM) was higher than average values from literature on grazing cattle. Moreover, it was positively related to the N 24H . Most of this N (29.5 ± 10.3 kg ha -1  yr -1 ) was translocated towards resting areas, which generally occurred on flat open pastures. Our results highlight the potential of Highland cattle to effectively translocate part of the ingested N from A. viridis -encroached towards targeted open areas, thus bringing new perspective for forage yield and quality improvement in the long-term., Supplementary Information: The online version contains supplementary material available at 10.1007/s10705-023-10282-0., Competing Interests: Competing interestsThe authors declare no competing interests., (© The Author(s) 2023.)

The beef livestock system in Sardinia is based on suckler cows, often belonging to autochthonous breeds, such as the Sarda breed, and they often graze silvopastoral areas. Besides beef meat, silvopastoral systems (SPSs) provide several Ecosystem Services (ESs), such as timber provision, harvested as wood, and watershed protection. Livestock distribution is a critical factor for the sustainable use of SPSs (e.g., to avoid uneven grazing patterns) and information on patterns of spatial use are required. A study was conducted to determine: (i) the spatial distribution and (ii) the habitat selection of Sarda cattle grazing in a Mediterranean silvopastoral area. Over different seasons, 12 free-roaming adult Sarda cows were fitted with Global Positioning System (GPS) Knight tracking collars to calculate an index mapping of the incidence of livestock in the landscape (LRI) and a preference index (PI) for different areas. Since the PI data were not normally distributed, the Aligned Rank Transform (ART) procedure was used for the analysis. LRI was able to represent the spatial variability in resource utilization by livestock as a LRI map. Overall, the areas where the animals drank and received supplementation were strongly preferred by the cows, reaching PI values in the summer of 19.3 ± 4.9 (median ± interquartile range), whereas areas with predominantly rocks were strongly avoided (the worst PI value in the spring was 0.2 ± 0.6). Grasslands were, in general, used in proportion to their presence in the area, with slightly increased use in the spring (PI 1.1 ± 0.5). Forest area was avoided by cows, except in the spring when it was used in proportion to their presence in the area.

Arsenic (As) represents an environmental risk and phytoremediation has been identified as a good technique to recover contaminated soils. Plants defense mechanisms needed to be enhanced against As stress-promoting action by biostimulants such as humic materials. This work sought to determine the effectiveness of an alkaline vermicompost extract (AEV) and in mitigating stresses promoted by As in maize plants, increasing their potential use for phytoextraction. The AEV were extracted from vermicompost and two preliminary assays in Leonard pots were carried out: the first one to define the best AEV concentration-response dose and the second to point out the toxic As concentration. The second step was to set up a 28-day long experiment with the following four treatments: control, AEV, As, As + AEV. AEV attenuated As-induced stress in maize plants. Maize dry biomass was reduced in the As treatment and rebalanced to values similar to the control in the As + HS treatment while the plants treated only with HS showed the highest biomass among the treatments. The concentrations of P, Fe, Cu, Mn and Ni, and catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) antioxidant activity increased in the As treatment and decreased in the As + AEV treatment. The rate of photosynthesis decreased, and the internal CO 2 concentration increased with stress induced by As, where both effects were attenuated by AEV. Our results show the positive effect of the AEV in alleviating As abiotic stress on maize growth, offering new options of employment of humic substances in phytoremediation process., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Regardless of the issue, most of the research carried out on summer pastures of European Alps had to consider the effects of grazing management, as it is an intrinsic component of alpine environment. The management intensity of grazing livestock is measured in terms of livestock stocking rate, but not always a direct measure of it is easily retrievable. Therefore, the aim of the research was to test the reliability of proxies easily retrievable from open data sources (i.e. slope and distance from buildings) in approximating the pastoral site-use intensity. To test the proxies' effectiveness two different approaches were used. With the first one, the proxies' reliability was assessed in a case-study conducted at farm scale by using the number of positions gathered with GPS collars, which are a reliable measure of livestock site-use intensity. With the second, the proxies' reliability was assessed by means of five Vegetation Ecological Groups (VEGs), used as a tool for indirect quantification of livestock site-use intensity at regional scale (thirty-two alpine valleys of the Western Italian Alps, Piedmont Region-Italy). At farm scale, distance from buildings and slope were both reliable predictors of the number of GPS locations as assessed with a Generalized Additive Model. Results of Generalized Linear Models at the regional scale showed that the values of both the slope and the distance from buildings were able to separate VEGs along the same site-use intensity gradient assessed by modelling the number of GPS locations at farm scale. By testing proxies' reliability both with a direct (i.e. GPS collar positions) and indirect (i.e. VEGs) measurement of livestock site-use intensity, results indicated that slope and distance from buildings can be considered effective surrogates of site-use intensity gradient in alpine grasslands managed under livestock grazing. Therefore, when the level of site-use intensity in research carried out in alpine summer pastures is not directly available, a reliable solution consists in the use of the terrain slope and the distance from buildings, which are also easily retrievable from open data sources or computable., Competing Interests: The authors have declared that no competing interests exist.

Objective: This prospective case series aims to evaluate the clinical impact of a hypochlorous acid oxidising solution (AOS) in association with usual standard of care (SoC) on wound bed preparation (WBP) in patients with hard-to-heal ulcers of various aetiologies. The AOS (Nexodyn, APR Applied Pharma Research S.A., Switzerland) comprises three main features: highly pure and stabilised hypochlorous acid, acidic pH and high reduction-oxidation potential., Method: Between February 2015 and February 2017, patients who met the inclusion criteria were treated with AOS and usual SoC. Data collection ran for 70 days: T0-T70. A baseline assessment was undertaken at T0; parameters assessed at fortnightly visits included: WBP score, area and depth of ulcer, duration, pain, Bates-Jensen score and infection status., Results: A total of 60 patients took part in the study. By T70, 68.3% of wounds had healed or improved and a significant wound size reduction of 21% was observed (p<0.001), despite a mean wound duration of 20.6 months. All wounds were free of local infection and cellulitis; 10% were colonised. WBP scores improved, while Bates-Jensen and pain scores fell significantly over time., Conclusion: This evaluation suggests that AOS might represent a valuable therapeutic addition for an optimal WBP in the routine management of hard-to-heal ulcers of different aetiologies., Declaration of Interest: ER worked as a consultant for APR Applied Pharma Research S.A. The authors have no other conflicts of interest.

Objective: Immediately following a two-year prospective case series in which the wounds of 60 patients with hard-to-heal ulcers were treated with a hypochlorous acid oxidising solution (AOS) in addition to standard of care (SoC) for 70 days (T0-T70), a subset of 31 patients (51.7%) whose wounds had not fully healed by T70 opted to continue with treatment for a further 22 weeks (days T70-T224, a total treatment time of 32 weeks (224 days). The objective was to provide long-term evidence on the clinical performance and safety of AOS when used in association with the usual SoC in patients with stalled, hard-to-heal ulcers of various aetiologies., Method: As per the main study, wounds were formally assessed by the study lead at 28 days (±14 days, depending on patient attendance). Parameters assessed at fortnightly visits included area, depth and duration of ulcer; pain; wound bed preparation (WBP) score; and infection status. Wounds were managed in accordance with the SoC protocol., Results: By T224, 35.5% (n=11) of wounds healed completely and 83.9% showed some types of improvement. All wounds were free of infection and colonisation, the WBP score improved (100% A1-A2 at T196), and pain scores fell. Use of AOS in combination with several types of dressing (SoC) for such a long period confirmed a good safety profile., Conclusion: This follow-up evaluation, coupled with the primary study, suggests that AOS might represent a valuable therapeutic addition for the management of hard-to-heal ulcers for long periods of treatment., Declaration of Interest: ER worked as a consultant for APR Applied Pharma Research S.A. The authors have no other conflicts of interest.

Objective: To report the clinical validation of an innovative, artificial intelligence (AI)-powered, portable and non-invasive medical device called Wound Viewer. The AI medical device uses dedicated sensors and AI algorithms to remotely collect objective and precise clinical data, including three-dimensional (3D) wound measurements, tissue composition and wound classification through the internationally recognised Wound Bed Preparation (WBP) protocol; this data can then be shared through a secure General Data Protection Regulation (GDPR)- and Health Insurance Portability and Accountability Act (HIPAA)-compliant data transfer system. This trial aims to test the reliability and precision of the AI medical device and its ability to aid health professionals in clinically evaluating wounds as efficiently remotely as at the bedside., Method: This non-randomised comparative clinical trial was conducted in the Clinica San Luca (Turin, Italy). Patients were divided into three groups: (i) patients with venous and arterial ulcers in the lower limbs; (ii) patients with diabetes and presenting with diabetic foot syndrome; and (iii) patients with pressure ulcers. Each wound was evaluated for area, depth, volume and WBP wound classification. Each patient was examined once and the results, analysed by the AI medical device, were compared against data obtained following visual evaluation by the physician and research team. The area and depth were compared with a Kruskal-Wallis one-way analysis of variations in the obtained distribution (expected p-value>0.1 for both tests). The WBP classification and tissue segmentation were analysed by directly comparing the classification obtained by the AI medical device against that of the testing physician., Results: A total of 150 patients took part in the trial. The results demonstrated that the AI medical device's AI algorithm could acquire objective clinical parameters in a completely automated manner. The AI medical device reached 97% accuracy against the WBP classification and tissue segmentation analysis compared with that performed in person by the physician. Moreover, data regarding the measurements of the wounds, as analysed through the Kruskal-Wallis technique, showed that the data distribution proved comparable with the other methods of measurement previously clinically validated in the literature (p=0.9)., Conclusion: These findings indicate that remote wound assessment undertaken by physicians is as effective through the AI medical device as bedside examination, and that the device was able to assess wounds and provide a precise WBP wound classification. Furthermore, there was no need for manual data entry, thereby reducing the risk of human error while preserving high-quality clinical diagnostic data.

Mangrove areas are among most threatened tropical ecosystems worldwide. Among polluting agents Cadmium is often found in high concentrations in mangrove sediments. Humic substances, complex biomolecules formed in soil and sediments during animal and plant residuals decomposition, have a known biostimulant activity and can be adopted to counteract various plant stresses. This study explores, in controlled conditions, the effect of humic substances on Avicennia germinans seedlings, with or without cadmium contamination. Humic compounds significantly changed plant root architecture, and, when coupled with cadmium, root anatomy and Cortex to Vascular Cylinder diameter ratio. These modifications led to lower Cd uptake by humic substances-treated plants. Humic substances amendment could be effective, depending on their concentrations, on improving plant health in mangrove areas, for forest recuperation and/or dredged sediments phytoremediation purposes., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

One of the industrial pillars of Espírito Santo state, South East of Brazil, is iron-mining products processing. This activity brings to a high level of coastal pollution due to deposition of iron particulate on fragile ecosystems as mangroves and restinga. Schinus therebinthifolius (aroeira) is a widespread restinga species. This work tested iron toxicity alleviation by vermicompost humic substances (HS) added to aroeira seedlings in hydroponic conditions. Catalase, peroxidase, and ascorbate peroxidase are antioxidant enzymes that work as reactive oxygen species (ROS) scavengers: they increase their activity as an answer to ROS concentration rise that is the consequence of metal accumulation or humic substance stimulation. S. terebinthifolius seedlings treated with HS and Fe augmented their antioxidant enzyme activities significantly less than seedlings treated separately with HS and Fe; their significantly lower Fe accumulation and the slight increase of root and leaf area confirm the biostimulating effect of HS and their role in blocking Fe excess outside the roots. The use of HS can be useful for the recovery of areas contaminated by heavy metals.

The growing problem of remediation of contaminated sediments dredged from harbor channels needs to be resolved by a cost effective and sustainable technology. Phytoremediation, by ex situ remediation plants, seems to have the potential to replace traditional methods in case of moderately contaminated sediments. On the other side, the need to mix sediments with soil and/or sand to allow an easier establishment of most employed species causes an increase of the volume of the processed substrate up to 30%. Moreover the majority of phytoremediating species are natives of temperate climate belt. Mangroves, with a special focus on the genus Avicennia - a salt secreting species - should represent an effective alternative in terms of adaptation to salty, anoxic sediments and an opportunity to develop ex situ phytoremediation plants in tropical and subtropical regions. The use of humic acid to increase root development, cell antioxidant activity and the potential attenuation of the "heavy metals exclusion strategy" to increase phytoextraction potentials of mangroves will be reviewed., (Copyright © 2017. Published by Elsevier Ltd.)

Premise of the Study: Are there dimensions of symbiotic root interactions that are overlooked because plant mineral nutrition is the foundation and, perhaps too often, the sole explanation through which we view these relationships? In this paper we investigate how the root nodule symbiosis in selenium (Se) hyperaccumulator and nonaccumulator Astragalus species influences plant selenium (Se) accumulation., Methods: In greenhouse studies, Se was added to nodulated and nonnodulated hyperaccumulator and nonaccumulator Astragalus plants, followed by investigation of nitrogen (N)-Se relationships. Selenium speciation was also investigated, using x-ray microprobe analysis and liquid chromatography-mass spectrometry (LC-MS)., Key Results: Nodulation enhanced biomass production and Se to S ratio in both hyperaccumulator and nonaccumulator plants. The hyperaccumulator contained more Se when nodulated, while the nonaccumulator contained less S when nodulated. Shoot [Se] was positively correlated with shoot N in Se-hyperaccumulator species, but not in nonhyperaccumulator species. The x-ray microprobe analysis showed that hyperaccumulators contain significantly higher amounts of organic Se than nonhyperaccumulators. LC-MS of A. bisulcatus leaves revealed that nodulated plants contained more γ-glutamyl-methylselenocysteine (γ-Glu-MeSeCys) than nonnodulated plants, while MeSeCys levels were similar., Conclusions: Root nodule mutualism positively affects Se hyperaccumulation in Astragalus. The microbial N supply particularly appears to contribute glutamate for the formation of γ-Glu-MeSeCys. Our results provide insight into the significance of symbiotic interactions in plant adaptation to edaphic conditions. Specifically, our findings illustrate that the importance of these relationships are not limited to alleviating macronutrient deficiencies., (© 2014 Botanical Society of America, Inc.)

Plastocyanin, which requires copper (Cu) as a cofactor, is an electron carrier in the thylakoid lumen and essential for photoautotrophic growth of plants. The Cu microRNAs, which are expressed during Cu deprivation, down-regulate several transcripts that encode for Cu proteins. Since plastocyanin is not targeted by the Cu microRNAs, a cofactor economy model has been proposed in which plants prioritize Cu for use in photosynthetic electron transport. However, defects in photosynthesis are classic symptoms of Cu deprivation, and priorities in Cu cofactor delivery have not been determined experimentally. Using hydroponically grown Populus trichocarpa (clone Nisqually-1), we have established a physiological and molecular baseline for the response to Cu deficiency. An integrated analysis showed that Cu depletion strongly reduces the activity of several Cu proteins including plastocyanin, and consequently, photosynthesis and growth are decreased. Whereas plastocyanin mRNA levels were only mildly affected by Cu depletion, this treatment strongly affected the expression of other Cu proteins via Cu microRNA-mediated transcript down-regulation. Polyphenol oxidase was newly identified as Cu regulated and targeted by a novel Cu microRNA, miR1444. Importantly, a spatiotemporal analysis after Cu resupply to previously depleted plants revealed that this micronutrient is preferentially allocated to developing photosynthetic tissues. Plastocyanin and photosynthetic electron transport efficiency were the first to recover after Cu addition, whereas recovery of the other Cu-dependent activities was delayed. Our findings lend new support to the hypothesis that the Cu microRNAs serve to mediate a prioritization of Cu cofactor use. These studies also highlight poplar as an alternative sequenced model for spatiotemporal analyses of nutritional homeostasis.