5,593 results on '"Plant Extract"'
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2. Notoginsenoside R1, a metabolite from Panax notoginseng (Burkill) F.H.Chen, stimulates insulin secretion through activation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway.
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Al-Romaiyan, Altaf, Barakat, Ahmad, Marafie, Sulaiman K., and Masocha, Willias
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Background: For ages, botanical medicine has been used in the treatment of diabetes mellitus (DM). Notoginsenoside R1 (NGR1), a Panax notoginseng (Burkill) F.H.Chen metabolite, has been documented to possess antidiabetic action in vivo. However, its precise molecular mechanism of action is not clear. Objectives: We evaluated NGR1's effects on blood glucose in vivo and then evaluated in vitro whether NGR1 has effects on insulin secretion and the probable molecular pathways involved in NGR1-induced insulin secretion. Methods: Diabetes was induced in mice by streptozotocin. Glucose tolerance test was performed before and after NGR1 was administered intraperitoneally to diabetic animals for 4 weeks. Static and perifusion experiments were performed using isolated female BALB/c mouse islets. Preproinsulin (Ins) mRNA expression was measured using q-PCR. Protein expression of PI3K/Akt pathway was assessed using the fully automated Wes• capillary-based protein electrophoresis. Results: Treatment of diabetic mice with NGR1 improved their glucose intolerance. In vitro, NGR1 increased insulin secretion in a concentration-dependent manner. NGR1 initiated the secretion of insulin at 2 mM glucose and augmented glucose-stimulated insulin secretion which was sustained throughout NGR1 perifusion. NGR1-induced insulin secretion was not altered by a voltage gated calcium channel blocker or protein kinase A inhibitor. NGR1 did not significantly modulate Ins mRNA expression. However, NGR1 significantly increased the levels of phospho-Akt and phopho-p-85. Conclusion: In conclusion, this study has shown that NGR1 ameliorates hyperglycemia in diabetic mice. NGR1 has a direct insulin secretagogue activity on mouse islets, stimulates insulin secretion at both basal and postprandial glucose concentrations, and activates PI3K/Akt pathway to induce insulin secretion. These results suggest that NGR1 may provide an alternative therapy to manage DM. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Cryptocarya moschata extract decreases single and mixed biofilms on acrylic resins.
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Tasso, Camilla Olga, Ribas, Beatriz Ribeiro, Ferrisse, Túlio Morandin, de Oliveira, Jonatas Silva, and Jorge, Janaina Habib
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ANTIBIOTICS , *BIOFILMS , *DATA analysis , *RESEARCH funding , *STREPTOCOCCUS mutans , *DENTURES , *NYSTATIN , *KRUSKAL-Wallis Test , *DESCRIPTIVE statistics , *PLANT extracts , *CANDIDA albicans , *ANTI-infective agents , *ANALYSIS of variance , *STATISTICS , *INFERENTIAL statistics , *MICROSCOPY , *CELL survival , *DATA analysis software , *ACRYLIC resins , *PENICILLIN - Abstract
Objective: This study proposed to assess the effect of Cryptocarya moschata extract on single and mixed biofilms formed on denture base and reline acrylic resin. Materials and Methods: Single and mixed biofilms of Candida albicans and Streptococcus mutans were formed on the samples and treated with C. moschata extract; Nystatin solution at 100,000 IU/mL or Penicillin antibiotic solution at 100,000 IU/mL; or PBS solution. Antimicrobial activity was analyzed by counting colony‐forming units, metabolism assay, assessment of protein components of the biofilm matrix, and of cell viability using confocal laser scanning microscopy (CLSM). Data were submitted to ANOVA and Tukey's post‐test (α = 0.05). Results: Cryptocarya moschata extract reduced cell viability of C. albicans and S. mutans single and mixed biofilms formed on samples. For all types of biofilms in the C. moschata group, there was a log reduction of the biofilm, proven by the Alamar Blue assay. Analyzing the extracellular matrix protein components, groups treated with the extract exhibited a lower level of fluorescence compared to the PBS groups. Reduction in thickness biofilm and viable cells was perceptible in the C. moschata group when assessing through CLSM. Conclusion: Cryptocarya moschata extract reduced the single and mixed biofilms of C. albicans and S. mutans on acrylic resins. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Evaluation of efficacy of four Cinnamomum species extracts and cinnamaldehyde to control anthracnose of mango fruit.
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Leesutthiphonchai, Wiphawee, Piasai, Onuma, Vajrodaya, Srunya, Umrung, Sarinna, Schinnerl, Johann, Steinkellner, Siegrid, and Khewkhom, Netnapis
- Abstract
Anthracnose of mango is one of the major postharvest diseases of mango fruit caused by members of the Colletotrichum gloeosporioides species complex such as Colletotrichum siamense. Crude extracts from dry trunk bark of four Cinnamomum species (C. burmanni, C. iners, C. loureiroi, and C. verum), a commercial cinnamon powder, cinnamaldehyde, eugenol, and cinnamon oil were assayed for their antifungal activity against Colletotrichum siamense. The crude extract of C. verum at 500 mg L
−1 showed the highest inhibition of mycelial growth. At a concentration above 10 g L−1 cinnamaldehyde, eugenol, and cinnamon oil showed 100% mycelial inhibition. Using the microdilution assay, C. burmanni and C. verum crude extracts were effective against Colletotrichum siamense spore germination and showed a minimum inhibitory concentration (MIC) value of 625 mg L−1 while the MIC value of cinnamaldehyde was 50 mg L−1 . The direct bioautography of the C. verum extract and the fractions obtained by column chromatography over silica gel against Cladosporium herbarum revealed clear inhibition zones on TLC plates. The treatment of Colletotrichum siamense spores with this active fraction led to severe membrane damage which was observed by scanning electron microscopy. Comparative HPLC analyses of the Cinnamomum extracts and the active fraction of C. verum, cinnamon power, and the cinnamaldehyde and eugenol as standards indicated cinnamaldehyde as the major compound. The C. verum fraction reduced disease severity and disease incidence on inoculated mango fruit. Moreover, uninoculated mango dipped into C. burmanni and C. verum extracts reduced the naturally occurring disease while total soluble solid, titratable acidity, and weight loss of dipped mango were insignificantly different from the untreated fruit control. [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. Green Synthesis and Evaluation of ZnO NPs and study the effect of Their toxic on Honey Bee (Apis mellifera).
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Karim, Ayoub Sabir, Abdullah, Fuad Othman, and Mohammad, Najat Zaid
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HONEYBEES ,FOURIER transform infrared spectroscopy ,PARSLEY ,METALLIC oxides ,PLANT extracts - Abstract
Copyright of Baghdad Science Journal is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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6. Herbal Amara extract induces gastric fundus relaxation via inhibition of the M2 muscarinic receptor.
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Piqué‐Borràs, Maria‐Riera, Röhrl, Johann, and Künstle, Gerald
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Background Methods Key Results Conclusion and Inferences Impaired gastric accommodation is one of the most frequent symptoms of functional dyspepsia. The safety and efficacy of conventional treatments remain to be proven and alternative herbal therapies have been proposed to alleviate gastrointestinal symptoms. This preclinical study examined the role of herbal Amara extract (containing Artemisia absinthium, Centaurium erythraea, Cichorium intybus, Gentiana lutea, Juniperus communis, Achillea millefolium, Peucedanum ostruthium, Salvia officinalis, and Taraxacum extracts) on gastric (fundus) accommodation and the possible implication of muscarinic receptors in its regulation.The effect of Amara extract on fundus motility was investigated in organ baths of smooth muscle strips isolated from the fundus of guinea pigs, and the role of the muscarinic receptor pathway was evaluated using functional and radioligand binding assays in cell lines expressing the M2 or M3 muscarinic receptor.Amara extract inhibited carbachol‐induced contraction of guinea pig smooth muscle strips in a dose‐dependent manner. This relaxant effect was not affected by the M3 antagonist J‐104129. Amara extract also inhibited M2, but not M3, receptor activity in CHO‐K1 cells (IC50 219 μg mL−1), and specifically bound the M2 receptor (IC50 294 μg mL−1). Of the nine herbal components of Amara extract, Juniperus communis, P. ostruthium, and Salvia officinalis inhibited M2 receptor activity (IC50 32.0, 20.8, and 20.1 μg mL−1, respectively), and P. ostruthium was sufficient to reverse carbachol‐induced ex vivo contraction of guinea pig fundic smooth muscles.Amara extract relaxes gastric smooth muscles by inhibiting the M2 muscarinic receptor. This study suggests the potential benefit of Amara extract for patients with impaired gastric accommodation. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Effect of UV Radiation and Temperature on Radical Scavenging Activity of Hippophaë rhamnoides L. and Vaccinium oxycoccos L. Fruit Extracts.
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Michalak, Monika, Pilawa, Barbara, Ramos, Paweł, and Glinka, Ryszard
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ELECTRON paramagnetic resonance , *FREE radicals , *ELECTRON paramagnetic resonance spectroscopy , *ULTRAVIOLET radiation , *SKIN aging - Abstract
New active ingredients, including those of plant origin, which could protect the skin against various harmful factors, such as UV radiation and free radicals responsible for skin ageing, are still being sought. The present study was focused on the antioxidant activity of Hippophaë rhamnoides L. and Vaccinium oxycoccos L. fruit glycolic extracts. Investigations were also carried out to evaluate the effect of UVA radiation and the storage of the sea buckthorn and European cranberry extracts at an elevated temperature of 50 °C on their interactions with free radicals. The kinetics of the interactions of the extracts with DPPH were assessed using electron paramagnetic resonance (EPR) spectroscopy. The sea buckthorn and European cranberry extracts quench the EPR signal of DPPH free radicals, which indicates their antioxidant potential. The EPR method further showed that a mixture of sea buckthorn and cranberry extracts in a volume ratio of 2:1 was more potent in quenching free radicals compared to a mixture of these extracts in a ratio of 1:2. Our findings demonstrate that long-term UVA radiation exposure reduces the ability of sea buckthorn and cranberry extracts to interact with free radicals. Moreover, storage at elevated temperatures does not affect the interaction of sea buckthorn extract with free radicals, while it alters the ability of cranberry extract to interact with free radicals. This study has demonstrated that an important factor in maintaining the ability to scavenge radicals is the storage of raw materials under appropriate conditions. H. rhamnoides and V. oxycoccos extracts can be used as valuable raw materials with antioxidant properties in the pharmaceutical and cosmetic industries. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Key role of Phyllanthus emblica L. fruit extract promotes ZVI/H2O2 process: rich titratable acid, suitable chelating ability, and antioxidant capacity.
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Lu, Baizhou, Fang, Zhanqiang, and Tsang, Pokeung Eric
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OXIDANT status ,FRUIT extracts ,HETEROGENEOUS catalysts ,PLANT extracts ,SURFACE reactions - Abstract
Phyllanthus emblica L. fruit extract (PFE) was introduced to improve ZVI/H
2 O2 technology, and the efficiency and mechanism of PFE promoting ZVI/H2 O2 technology were explored. With the introduction of PFE, the Norfloxacin (NOR) removal rate and kobs of the process were improved by 41.17% and 5.08 times, respectively. In the ZVI/H2 O2 /PFE process, the degradation of NOR by the attack of ROS is the main pathway for decontamination and is dominated by the heterogeneous reaction on the catalyst surface. PFE contains 13.92 g/L titratable acid and has good complexing ability and antioxidant ability. The mechanism of PFE promoting ZVI/H2 O2 technology was based on lowering the pH, complemented by chelation and antioxidant capacity. With the introduction of PFE, the utilization rate of the reagent was significantly increased (7.56 times for ZVI and 3.21 times for H2 O2 ), the applicable pH range was widened (6–9) and the iron sludge was reduced (32.80%). Meanwhile, the concept of UPR is proposed for the first time. The result is the key role to the selection of green promoters in the ZVI/H2 O2 process depends on the abundance of titratable acid, followed by a certain chelating ability and antioxidant capacity. [ABSTRACT FROM AUTHOR]- Published
- 2024
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9. Evaluation of inhibitory activity of essential oils and natural extracts on foodborne viruses.
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Amri, Mariem, Jubinville, Éric, Goulet-Beaulieu, Valérie, Fliss, Ismail, and Jean, Julie
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GRAPE seed extract , *PLANT products , *PLANT viruses , *ESSENTIAL oils , *PLANT extracts - Abstract
Aims Enteric viruses are recognized as a major concern in health care and in the food sector in Canada. Novel clean-label strategies for controlling enteric viruses are sought in the food industry. In this study, we examined the antiviral potential of plant extracts and essential oils on murine norovirus 1 (MNV-1), hepatitis A virus (HAV), and herpes simplex virus 1 (HSV-1). Methods and results Inactivation of the viruses by grape seed, blueberry, green tea, and cranberry extracts and by rosemary and thyme essential oils was measured using plaque formation assay. Concentrations ranging from 50 to 200 000 ppm with a contact time of 90 min were tested. Grape seed extract at 10 000 ppm was the most effective (P < 0.05) at reducing MNV-1 and HAV infectious titers, respectively, by 2.85 ± 0.44 log10 and 1.94 ± 0.17 log10. HSV-1 titer was reduced by 3.81 ± 0.40 log10 at 1000 ppm grape seed extract. Conclusions Among the plant products tested, grape seed extract was found the most effective at reducing the infectious titers of MNV-1, HAV, and HSV. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Use of Conyza canadensis L. Extracts as Biostimulant in Cyclamen persicum Mill.
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Batista, Eunice R., May, Andre, Procópio, Sergio O., Assalin, Marcia R., Quevedo, Helio D., Binhardi, Nicole, and Queiroz, Sonia C. N.
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PLANT extracts , *GAS exchange in plants , *WATER efficiency , *SUSTAINABILITY , *SUSTAINABLE agriculture - Abstract
Cyclamen (Cyclamen persicum Mill.) is an ornamental plant that is highly susceptible to pathogens, requiring high amounts of phytosanitary products. Therefore, the development of more sustainable alternatives has been required. The present study aimed to analyze the effect of C. canadensis root extract (aqueous and with dichloromethane) applied via foliar or soil, in C. persicum, on gas exchange and the SPAD index and on the biomass of cyclamen. The aqueous extract treatment increased net CO2 assimilation, the transpiration rates, and instantaneous carboxylation efficiency. The water use efficiency values were reduced in the treatments with both extracts. The greatest increases in the SPAD index were provided by the aqueous extract. The cyclamens that received the aqueous extract applied in soil or the dichloromethane extract applied in leaves showed an increase in total biomass and number of leaves. To identify the compounds present in the extracts, CG-MS and LC-MS/MS analyses were performed. The positive effects obtained indicated a high biostimulant effect of C. Canadensis. Thus, the root extracts of C. Canadensis, particularly the aqueous extracts, have the potential to be used to reduce the use of mineral fertilizers and pesticides, promoting agroecological practices and contributing to sustainable agriculture. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Biosynthesis of Zinc Oxide Nanoparticles Using Garlic Peel Extract and Their Antibacterial Potential.
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Abdelmoteleb, Ali, Valdez-Salas, Benjamín, Beltran-Partida, Ernesto, Mendez-Trujillo, Vianey, González-Mendoza, Daniel, Tzintzun-Camacho, Olivia, and Roumia, Ahmed F.
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FOURIER transform infrared spectroscopy , *ABSORPTION spectra , *ZINC oxide , *X-ray spectroscopy , *BACILLUS cereus - Abstract
Zinc oxide nanoparticles (ZnO NPs) have gathered interest because of their unique characteristics and potential applications. In the current work, ZnO NPs underwent an eco-friendly biosynthesis process using garlic peel extract. The biosynthesized ZnO NPs were characterized using different analyses including Ultraviolet-visible (UV-vis) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). The produced ZnO NPs exhibited a UV–vis spectrum absorption peak at 365 nm, thus indicating the formation of ZnO NPs. The SEM showed that the biosynthesized ZnO NPs had an irregular surface morphological shape with an average size of 17 nm, according to the DLS analysis. Based on the FTIR findings, the bioactive functional groups responsible for stabilizing and capping the ZnO-NPs were confirmed. The biosynthesized ZnO NPs exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and antimicrobial activities against Gram-positive (Bacillus cereus) and Gram-negative bacteria (Klebsiella pneumonia). Therefore, the plant-mediated biosynthesized ZnNPs can be considered a promising candidate as an antioxidant and antimicrobial agent against pathogenic microbes found in different areas such as food safety and agriculture. Through the utilization of bioinformatics, we identified six potential targets for drug development in K. pneumonia and B. cereus, along with their corresponding interacting residues with zinc oxide nanoparticles. Additionally, our research revealed that the zinc oxide nanoparticles exhibited binding capabilities with the sulfiredoxin domain located at the specific targets of K. pneumonia, a crucial mechanism responsible for the repair of bacterial cells under oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Phytochemical Screening and Evaluation of Antibacterial, Anticandidal, and Sporicidal Properties of Euphorbia tirucalli Extract in Terengganu, Malaysia.
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Wahab, Noor Zarina Abd, Malza, Nur Maizatul Najwa, and Rukayadi, Yaya
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EUPHORBIA , *CARDIAC glycosides , *GRAM-negative bacteria , *GRAM-positive bacteria , *EXTRACTS , *SHRUBS - Abstract
Euphorbia tirucalli, commonly known as the pencil cactus or milk bush plant, is used as an alternative medicine. The current study evaluated the phytochemical contents, antibacterial, anticandidal, and antisporicidal potential of the E. tirucalli methanolic extract. The antibacterial and anticandidal activity of E. tirucalli methanolic extract was determined by performing a disc diffusion assay, MIC, MBC, and MCC. The sporicidal activity was tested at different concentrations of extract and exposure times. Phytochemical analyses revealed the presence of alkaloids, saponins, cardiac glycosides, terpenoids, and tannins in E. tirucalli methanolic extract. Results demonstrated inhibition zones of the extract against Gram-positive bacteria were in range of 22.00-7.00 mm. Meanwhile, inhibition zones of the extract against Gram-negative bacteria were in range of 13.00-7.00 mm. All bacteria were inhibited with MIC values at 1.56-25.0 mg/ml and can be completely killed with MBC values of 20-200 mg/ml. Inhibition zones of E. tirucalli methanolic extract against Candida spp. were in the range of 20.00-8.00 mm. All Candida spp. were inhibited with MIC values at 1.56-100.00 mg/ml and MBC values of 100-300 mg/ml. All concentrations of the extract inhibited all Bacillus spp. spores at different exposure times. In conclusion, the methanolic extract of E. tirucalli exhibits antibacterial, anticandidal, and sporicidal activities. The findings indicated that the methanolic extract of E. tirucalli has good potential for prospective nature-based antimicrobial agents. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Antimicrobial Effect of Curcumin Nanoparticles and Ferulago angulate Boiss Extract Against Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Wound Infections.
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Vatani, Elnaz, Shayestehpour, Mohammad, Motallebi, Mitra, Razmjoue, Damoun, Moosavi, Gholam Abbas, Khaledi, Azad, and Rahimi, Mohammadreza
- Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of burn infections. Due to the increase of resistance in this bacterium, it is necessary to find new antimicrobial compounds. This study aimed to determine the antimicrobial effect of Curcumin nanoparticles and Ferulago angulate Boiss extract MRSA isolated from burns and other skin infections. Firstly, in addition to phenotypic microbial tests, the PCR technique for identification of the mecA gene was used to confirm MRSA strains. The MIC and checkerboard methods were used to investigate curcumin nanoparticles and Ferulago angulate Boiss extract effects against bacterial growth rate alone and in combination. The biofilm inhibitory effect was determined through the microtitre plate method. Also, the real-time PCR technique examined relative changes in eno gene expression expression. The MTT assay evaluated the cellular toxicity. The data obtained was analyzed through SPSS software and suitable statistical tests. Curcumin nanoparticles and F. angulate extract inhibited the growth of MRSA strains at a concentration of 312.5–625 µg/mL, (MIC50 = 312.5). About 76.92% and 23.07% of clinical isolates showed indifferent and additive effects in combination with curcumin and F. angulate extract. The combination of curcumin and the mentioned extract decreased the eno gene expression compared to the 16S rRNA housekeeping gene. These two substances in combination and alone did not show any significant cytotoxic effects on HDF skin cell lines. In conclusion, curcumin nanoparticles and F.angulate extract showed some antibacterial effects against MRSA. Therefore, this compound should be considered with further tests against MRSA strains isolated from burn infections. [ABSTRACT FROM AUTHOR]
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- 2024
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14. The Role of Plant Extracts in Enhancing Nutrition and Health for Dogs and Cats: Safety, Benefits, and Applications.
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Guo, Xinzi, Wang, Yifei, Zhu, Zhaoxuan, and Li, Lian
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DIETARY patterns ,ANIMAL welfare ,ESSENTIAL oils ,PLANT extracts ,PETS ,TERPENES - Abstract
Simple Summary: Companion animals, like dogs and cats, are vital to modern families, and their health is greatly influenced by their diet. Plant extracts, including polyphenols and enzymes, are increasingly used in pet food for their health benefits, such as improving metabolism and gut health. Pet owners are now adding supplements like tea polyphenols, quercetin, and curcumin to their pets' diets. These natural extracts can replace harmful synthetic antioxidants, extending the shelf life and quality of pet food. Additionally, medical plants offer therapeutic benefits and can help prevent diseases, potentially reducing drug resistance. However, it is crucial to ensure the safety and proper metabolism of these extracts in pets. This review highlights the health benefits of plant extracts in pet diets, aiming to improve dietary practices and overall well-being of companion animals. Plant extracts, derived from various natural sources, encompass primary and secondary metabolites, which include plant polysaccharides, polyphenols, alkaloids, flavonoids, glycosides, terpenes, and volatile oils. These compounds exhibit a range of biological activities such as antioxidant, anti-inflammatory, and antimicrobial functions. Currently, polyphenols and other bioactive compounds are being incorporated into the diets of farm animals, fish, and pets to promote health benefits. Despite this, the application and potential of plant extracts in canine and feline nutrition have not been comprehensively explored. Many aspects of the mechanisms underlying the action of these plant metabolites remain to be analyzed and elucidated. Furthermore, leveraging natural plant extracts for the treatment of clinical conditions in dogs and cats is a crucial component of clinical nutrition. Consequently, this review aims to highlight the impact of plant extracts on overall health, gastrointestinal health, immune health, cardiovascular health, redox balance, and pathology in dogs and cats. [ABSTRACT FROM AUTHOR]
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- 2024
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15. The Production of Marandu Grass (Urochloa brizantha) Extracts as a Natural Modifier of Rumen Fermentation Kinetics Using an In Vitro Technique.
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Freitas, Rafaela Scalise Xavier de, da Silva, Janaina Silveira, Furtado, Althieres José, Perna Junior, Flavio, de Oliveira, Alessandra Lopes, and Bueno, Ives Cláudio da Silva
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PROPIONIC acid ,PLANT extracts ,SIGNALGRASS ,FERMENTATION ,FACTORIALS ,RUMEN fermentation - Abstract
The ethanolic (EE) and hydroalcoholic (HE) extracts of Urochloa brizantha concentrations were developed with the aim of evaluating their effect on rumen fermentation using an in vitro gas production technique. The EE and HE presented 3.62 and 5.38 mg protodioscin/mL, respectively. Ten treatments were evaluated in a completely randomized factorial arrangement (2 × 4 + 2), where the main effects were two extracts (EE and HE) and four levels (50, 100, 150, and 200 mL of extract/kg of DM) plus two controls: one positive (25 ppm of monensin–MON) and one (with no additives–CTL). The extract treatments (EXT, EE, and HE) reduced colonization time by 33.59% compared to the MON. IVDMD (p < 0.001) and IVOMD (p < 0.0001) were negatively affected by EXT addition when compared to CTL. Additionally, EXT reduced the proportion of propionic acid and increased the proportion of butyric acid in relation to CTL and MON treatments. Both EE and HE extracts of U. brizantha were able to alter rumen fermentation kinetic, with HE showing a higher concentration of protodioscin. Further research is needed to optimize extraction methodologies, comprehensively profile secondary compounds, and conduct trials with varying doses to effectively assess the viability of U. brizantha extract as an additive. [ABSTRACT FROM AUTHOR]
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- 2024
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16. First Report: Senna multijuga Subsp. multijuga (Fabales: Fabaceae) as an Attractant and Bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae).
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Wandri, Ruli, Alam, Samsu, Ayundra, Shervinia Dwi, Apriansa, Azharudin, Asmono, Dwi, Subeki, Subeki, Fitriana, Yuyun, Hasibuan, Rosma, and Suharjo, Radix
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PLANT identification ,PLANT extracts ,SEQUENCE analysis ,RHINOCEROSES ,SCARABAEIDAE ,PHEROMONE traps - Abstract
In 2020, a plant displaying promising attributes as both an attractant and bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae), known locally as "Hujan Emas", was discovered at PT Sampoerna Agro Tbk, Indonesia. Despite its potential, the identity of this plant remains unknown. This study aims to reveal the plant's identity and ascertain its potential as an attractant and bioinsecticide against O. rhinoceros. Identification was performed using morphological and sequence analyses of the MaturaseK, chloroplast rps16, and chloroplast rpl16 genes. Field observations were conducted over 9 months, focusing on the number of adult O. rhinoceros visiting the plant and the number captured using synthetic pheromone (ethyl 4-methyloctanoate) traps. Bioinsecticidal activity assessments focused on extracts from the plant's bark, stem, and leaves. Morphological characterization and sequence analysis conclusively affirmed the plant's identity as Senna multijuga (Fabales: Fabaceae). The total number of O. rhinoceros specimens obtained from S. multijuga was 1.6 times higher (836 beetles) than that obtained using the pheromone trap (512 beetles), suggesting that S. multijuga has better attractant capability than ethyl 4-methyloctanoate. The leaf extract exhibited insecticidal activity against larvae, while the stem and bark extracts demonstrated insecticidal activity against both larvae and adults. Larval mortality ranged from 1.25% to 3.75%, while adult mortality was notably higher, ranging from 12.5% to 25%. This is the first report on the capability of S. multijuga as an attractant and bioinsecticide against O. rhinoceros. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Phytochemical profiling and antibacterial activities of Ziziphora tenuior root extracts: a molecular docking against VanA of vancomycin-resistant enterococci.
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Hatami, Asma
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PLANT extracts , *TREATMENT effectiveness , *ESCHERICHIA coli , *MOLECULAR docking , *METABOLITES - Abstract
Medicinal plants, renowned for their antibacterial phytocompounds and secondary metabolites, hold significant promise in addressing antibiotic-resistant bacterial strains. This study aimed to conduct phytochemical profiling of the methanolic and dichloromethane extracts of Ziziphora tenuior root using the GC–MS technique. These extracts' antioxidant potential was assessed via DPPH assay and their antibacterial activity was evaluated against S. aureus, E. coli, and VRE bacterial strains. Furthermore, the drug–ligand interactions between the extracts' biocompounds and d-alanyl-d-lactate ligase (VanA) protein of vancomycin-resistant enterococci strains (VRE) were analyzed using molecular docking. Based on the results, 74% of methanolic extract consisted of (3methyl, 24S)-stigmast-5-en-3-ol (which is a β-sitosterol), followed by Tetrasiloxane, decamethyl (15.5%), and 1-methyl-4-phenyl-5-thioxo-1,2,4-triazolidin-3-one (10.5%). Also, the only predominant compound identified in the dichloromethane extract was Benzo[h]quinoline, 2,4-dimethyl-. Both extracts showed antioxidant activity, while the antioxidant activity of the methanolic extract (IC50 = 95.33 μg/ml) was significantly higher than that of the dichloromethane extract (IC50 = 934.23 μg/ml). Also, both extracts displayed substantial antibacterial efficacy against the tested pathogens, particularly against VRE. Moreover, the in silico analysis revealed that (3methyl, 24S)-stigmast-5-en-3-ol and Benzo[h]quinoline,2,4-dimethyl- exhibited notable interactions with VanA through docking energy values of − 9.0 and − 9.1 kcal/mol, respectively. Furthermore, these compounds formed 2 and 1 hydrogen bonds with VanA, respectively, highlighting their potential as effective interactants. These findings provide valuable visions into the therapeutic potentials of these plant-derived biocompounds in combating antibiotic-resistant bacterial infections. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Antimicrobial Activity against Cronobacter of Plant Extracts and Essential Oils in a Matrix of Bacterial Cellulose.
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Stasiak-Różańska, Lidia, Berthold-Pluta, Anna, Aleksandrzak-Piekarczyk, Tamara, Koryszewska-Bagińska, Anna, and Garbowska, Monika
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PLANT extracts , *ESSENTIAL oils , *PACKAGING materials , *BIOACTIVE compounds , *CRONOBACTER , *THYMES - Abstract
Bacterial cellulose (BC) is a biodegradable polymer resembling paper after being dried. It finds a growing number of applications in many branches of industry and in medicine. In the present study, BC was produced after Gluconacetobacter hansenii ATCC 23769 strain culture and used as a matrix for plant extracts (tulsi, brahmi, lemon, blackberry, nettle root, and nettle leave) and essential oils (cinnamon, sage, clove, mint, thyme, lemongrass, rosemary, lemon, anise, tea tree, lime, grapefruit, and tangerine), and the antimicrobial properties of these biomaterials was determined. The growth-inhibiting effects of plant extracts and essential oils combined with BC were analyzed against five Cronobacter species isolated from food matrix and two reference strains from the ATCC (513229 and 29544). Additional analyses were conducted for BC water activity and for its capability to absorb biologically active plant compounds. The cellulose matrix with a 50% extract from brahmi was found to effectively inhibit the growth of the selected Cronobacter strains. The other plant water extracts did not show any antimicrobial activity against the tested strains. It was demonstrated that BC soaked with thyme essential oil was characterized with the strongest antimicrobial activity in comparison to the other tested EOs. These study results indicate the feasibility of deploying BC impregnated with natural plant components as an active and environmentally-friendly packaging material. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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19. Neuroprotective and antioxidant activities of Colombian plants against paraquat and C2-ceramide exposure in SH-SY5Y cells.
- Author
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Bustos-Rangel, Angie, Muñoz-Cabrera, Jonathan, Cuca, Luis, Arboleda, Gonzalo, Ávila Murillo, Mónica, and Sandoval-Hernández, Adrián G.
- Subjects
NEUROPROTECTIVE agents ,ANTIOXIDANTS ,CERAMIDES ,REACTIVE oxygen species ,PHYTOCHEMICALS - Abstract
Abnormal production of reactive oxygen species (ROS) has been implicated in the physiopathology of neuronal cell death. Increased ROS levels are associated with exacerbated peptide aggregation, inflammation, and mitochondrial dysfunction, which facilitate the triggering of specific cell death pathways. Antioxidant molecules are potentially useful in the amelioration of neurodegeneration. In this regard, natural products are an invaluable source of antioxidants. Therefore, we investigate the antioxidant and neuroprotective activities of four Colombian angiosperm extracts. Antioxidant activity was evaluated by phytochemical assays using TLC techniques with Dragendorff reagent, ninhydrin, and chloranil in dioxane, NH3, Fast Blue, and FeCl3, together with bioautography using DPPH and β-carotene. In vitro neuroprotective activity, cell death, and ROS accumulation were evaluated by MTT and flow cytometry in the SH-SY5Y cell line exposed to paraquat and C2-ceramide. We found that Zanthoxylum rhoifolium Lam, Zanthoxylum martinicense, Nectandra membranacea, and Nectandra reticulata extracts have antioxidant activity higher than quercetin under a β-carotene bleaching assay and protect SH-SY5Y cells against paraquat and C2-ceramide associated with a reduction in ROS. In conclusion, these extracts have a strong neuroprotective potential, and the precise mechanism requires more evaluation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
20. Evaluation of a new plant-based formulation to control Varroa mite ( Varroa destructor) in honey bee ( Apis mellifera) colonies.
- Author
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Rahimi, Ataollah and Parichehreh, Shabnam
- Subjects
- *
VARROA destructor , *HONEYBEES , *MITE control , *POLLINATION by insects , *OXALIC acid , *ACARICIDES - Abstract
Varroa destructor is a dangerous pest directly for beekeeping and indirectly for crops that require insect pollination. In recent years, the application of chemical pesticides has led to the occurrence of mite resistance and contamination of hive products. Plant extracts are considered as a suitable alternative to chemical acaricides for the Varroa mite control. Therefore, the present study aimed to investigate the acaricidal and insecticidal activity of extract of Prangos ferulacea plant against the Varroa mite and its host in the honey bee colonies in the climatic conditions of Kurdistan province from 2022 to 2024. The P. ferulacea plant was collected in June 2022 from different regions of Kurdistan province in the flowering stage, extracted and then its chemical compounds were identified using GC-MS. The present study was conducted in the form of a factorial experiment based on a completely random basic design with four treatments and seven replications. Before conducting the bioassay experiments, the experimental colonies were homogenized in terms of queen age, population (adults and brood), and honey storage. Also, the initial infestation rate of colonies with Varroa mites was evaluated for adult and brood. The results showed that the extract of 50% of P. ferulacea was relatively favorable acaricidal properties. So that, there was no significant difference with oxalic acid and Apistan treatments in terms of mite mortality percentage in the first time of spraying. Also, the result showed that the bee's mortality percentage was not more than 7% in any of the times of spraying with the 50% extract of the P. ferulacea, and there was observed a significant difference compared to the oxalic acid and Apistan treatments in terms of bee's mortality percentage (P<0.01). The queen superseding was not observed in any of the experimental treatments. According to the findings of the present research, the concentration of 50% of the extract P. ferulaceous can be suggested as a suitable alternative to synthetic acaricides and organic acids to control the Varroa mite in honey bee colonies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
21. Turning Waste into Treasure: Invasive Plant Ambrosia trifida L Leaves as a High-Efficiency Inhibitor for Steel in Simulated Pickling Solutions.
- Author
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Sun, Xin, Tian, Huiwen, Zou, Fangxin, Li, Weihua, Qiang, Yujie, and Hou, Baorong
- Subjects
- *
FOURIER transform infrared spectroscopy , *WETLAND plants , *SCANNING electron microscopes , *STEEL corrosion , *PLANT extracts - Abstract
High toxicity is the main reason for the limited application of traditional corrosion inhibitors. Herein, it is critical to find a green, efficient, and long-term stable alternative substitute for the hazardous and conventional corrosion inhibitor. Ambrosia trifida L is widely distributed in fields and riverside wetlands as an invasive plant in China. According to the concept of turning waste into treasure, the extract of Ambrosia trifida L leaves (ATL) has the potential to address this issue due to its natural origin and abundant presence of heterocyclic organics. Therefore, ATL, as a green corrosion inhibitor, is prepared for the first time via a simple water-based extraction method. FT-IR (Fourier transform infrared spectroscopy) and UV-Vis (UV-visible) indicate that ATL extract contains abundant heterocyclic organics with conjugated structures, which exhibit the potential to become a high-efficiency inhibitor. Notably, the active sites of ATL molecules and their interaction with Q235 steel at the molecular/atomic level are revealed via theoretical calculations. The highest Ebinding value observed for the major components in the ATL extract is 259.66 kcal/mol, implying a significant adsorption capacity. The electrochemical results verify that microdose ATL extract can prominently inhibit steel corrosion, and the highest inhibition efficiency (η) is 97.5% (1000 mg/L). Following immersion for 24 h, the η value is enhanced to 99.0%, indicating a reliable and long-term ATL extract protection film is formed on the steel surface in harsh acidic solutions. The results of the weight loss, SEM (scanning electron microscope), and LSCM (laser scanning confocal microscopy) are consistent with the above conclusions. Finally, this study anticipates providing theoretical support for developing novel green plant extract inhibitors and aiding in their application in industrial pickling environments. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
22. GREEN SYNTHESIS OF Fe/Ni/Cr OXIDE NANOPARTICLES USING COSTUS PICTUS PLANT EXTRACT: MICROSTRUCTURE AND BIOLOGICAL PROPERTIES.
- Author
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KUMAR, PRAKASH, RAMESH, M. R., DODDAMANI, MRITYUNJAY, and SURESH, JOGHEE
- Subjects
- *
PLANT extracts , *ESCHERICHIA coli , *AGAR , *SANITARY engineering , *HAZARDOUS substances , *NANOPARTICLES - Abstract
Superior qualities to bulk material and larger surface area to volume ratio are some of the primary reasons that account for the wide use of nanoparticles (NPs) in biological applications. Advancements in molecular-level engineering and environmental and health concerns are driving factors in the green synthesis of NPs. Plant-based NP synthesis provides a good alternative compared to hazardous physical and chemical techniques of synthesis of NPs. An experimental study is carried out on the green synthesis of Fe/Ni/Cr oxide NPs using Costus pictus extract. The study encompassed an array of characterization techniques, including UV–visible spectroscopy for absorption properties, X-ray diffraction (XRD) for crystallinity assessment, and Fourier-transform infrared (FTIR) spectroscopy to probe the presence of phenolic groups in flavonoids that influence NP formation. Surface morphology and composition were elucidated using scanning electron microscopy (SEM) with energy-dispersive spectroscopy (EDS), while transmission electron microscopy (TEM) affirmed the NPs' size (approximately 20 nm). The surface area of the NPs, 96.806 m2/g, was determined through BET analysis. Furthermore, the antibacterial potential of the NPs against various bacterial strains, including S. mutans, E. coli, P. aeruginosa, and S. aureus, was investigated using the agar well diffusion method. The NPs showed good antibacterial potential against bacterial species E. coli and P. aeruginosa. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
23. Utilization of Eco‐friendly Copper Oxide Nanoparticles and Iron Oxide Nanorods in Dye Removal from Real Textile Industry Effluent.
- Author
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Rather, Mohd Yousuf and Sundarapandian, Somaiah
- Subjects
- *
INDUSTRIAL wastes , *COLOR removal (Sewage purification) , *IRON oxide nanoparticles , *COPPER oxide , *RESPONSE surfaces (Statistics) - Abstract
Textile industry wastewater contaminated with dye effluents poses a significant environmental challenge. Numerous nanoparticles are used as adsorbents to treat similarly stimulated wastewater, but particularly nanomaterials synthesized through green methods have gained prominence. To assess their practical applicability in addressing real‐world textile wastewater pollution, studies on dye removal from authentic textile industrial effluents are recommended. As a result, a study focused on the removal of dye from real textile industrial effluent is conducted, and biosynthesized copper oxide nanoparticles and iron oxide nanorods are chosen as adsorbents. The investigation scrutinized the influence of adsorbent dosage, adsorbent‐adsorbate contact time, and wastewater pH on the percentage of dye adsorption. These findings indicate that increasing the adsorbent dosage and contact time leads to a higher percentage of dye removal. Notably, copper oxide nanoparticles exhibit superior dye removal efficiency at pH levels 5 and 7, outperforming the maximum dye removal efficiency of iron oxide nanorods at pH 12. The study achieved an impressive process efficiency of 95.24% for copper oxide nanoparticles and 62.5% for iron oxide nanorods. Response surface methodology (RSM) is employed for statistical data analysis and optimization of dye removal process parameters to maximize efficiency. Overall, the results demonstrate that biosynthesized nanomaterials offer a promising and effective solution for removing dyes from textile industrial wastewater. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
24. Preparation and characterization of gelatin‐pectin‐based active films incorporated with Styrax benzoin oleo gum resin.
- Author
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Bhatia, Saurabh, Jawad, Muhammad, Chinnam, Sampath, Al‐Harrasi, Ahmed, Shah, Yasir Abbas, Koca, Esra, Aydemir, Levent Yurdaer, Alam, Tanveer, Mohan, Syam, Zoghebi, Khalid, and Khalid, Asaad
- Subjects
ACTIVE food packaging ,GUMS & resins ,INFRARED spectroscopy ,SURFACE roughness ,INTERMOLECULAR interactions - Abstract
In this study, benzoin oleogum resin (BOGR) loaded antioxidant gelatin/pectin (GEPE) based films were developed and characterized for various parameters including mechanical, barrier, optical, film hydrophilicity/hydrophobicity, surface roughness, chemical, thermal, morphological and antioxidant properties. The incorporation of BOGR decreased water solubility, moisture content, tensile strength, and elongation at break. However, water permeability, opacity, thickness, and water hydrophobicity were increased. Moreover, SEM and AFM analysis confirmed that the films loaded with BOGR showed heterogeneous and rougher surfaces in comparison to blank film. The intermolecular interactions between GEPE and BOGR was confirmed by infrared spectroscopy. Thermal stability of the prepared BOGR loaded films was improved. Additionally, antioxidant activities of the film were significantly increased with increase in concentration of BOGR as indicated by ABTS and DPPH assays. Our findings indicate that BOGR loaded composite films could be used as an active material for food packaging applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
25. Applicability of edible Candelilla wax composite blended with Bryophyllumpinnatum extract to prolong shelf life of fruits/vegetables.
- Author
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Fatima, Amber, Tahir, Fatima, Mohyuddin, Ayesha, Munir, Tabassum, Nadeem, Sohail, Javed, Mohsin, Othman, Mohd Hafiz Dzarfan, Goh, Hui Hwang, Al-Hazmi, Hussein, Aziz, Faissal, Anouzla, Abdelkader, and Kurniawan, Tonni Agustiono
- Subjects
EDIBLE coatings ,COMPOSITE coating ,FOOD preservation ,MEDICINAL plants ,PLANT extracts - Abstract
Candelilla wax (CW) is a natural lipid source that can be used to make biodegradable and edible coatings and films for fruits. However, CW alone does not provide sufficient antimicrobial and antioxidant properties to prevent microbial spoilage and oxidative deterioration of fresh products. This study aims at evaluating the applicability of CW blended with Bryophyllum pinnatum extract (BPE), a plant with medicinal and phytochemical properties, to enhance the shelf life of fruits. CW and BPE were mixed at different ratios and applied as coatings on banana, cucumber, lemon, and apple. The coated fruits were stored at room temperature for several days and evaluated for weight loss (WL), pH, and physical appearance. The results showed that the edible CW coating mixed with B. pinnatum extract could extend the shelf-life of selected fruits by lowering their weight loss (WL), as compared to those without coating (p ≤ 0.05; paired t-test). It was observed that % WL of banana was significantly higher in the control group than in the coated banana (p ≤ 0.05; paired t-test) and that the highest WL (4.5%) was reached on the 4
th day. The lowest WL (2.7%) was observed in the banana with edible coating with methanolic extract (ECME) formulation. The coating formed a complex layer that blocked the movement of H2 O molecules through the selected fruits, preventing water loss. The smallest pH change was observed in the banana coated with the extract, indicating that the coating was suitable for extending the shell life of banana. Overall, CW enriched with BPE maintained the quality of their products for marketability. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
26. Potentials of roots, stems, leaves, flowers, fruits, and seeds extract for the synthesis of silver nanoparticles.
- Author
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Saifuddin, Nurul Nazirah, Matussin, Shaidatul Najihah, Fariduddin, Qazi, and Khan, Mohammad Mansoob
- Abstract
Silver nanoparticles (AgNPs) have gained significant attention in various applications due to their unique properties that differ from bulk or macro-sized counterparts. In the advancement of nanotechnology, a reliable, non-toxic, and eco-friendly green synthesis has widely been developed as an alternative method for the production of AgNPs, overcoming limitations associated with the traditional physical and chemical methods. Green synthesis of AgNPs involves the utilization of biological sources including plant extracts with silver salt as the precursor. The potential of phytochemicals in plant extracts serves as a reducing/capping and stabilizing agent to aid in the bio-reduction of Ag
+ ions into a stable nanoform, Ag0 . This review provides insights into the potentials of various plant parts like root, stem, leaf, flower, fruit, and seed extracts that have been extensively reported for the synthesis of AgNPs. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
27. Effects of phytogenic feed additives in growing and finishing pigs under different stocking density
- Author
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Hyun Ah Cho, Min Ho Song, Ji Hwan Lee, Han Jin Oh, Jae Woo An, Se Yeon Chang, Dong Cheol Song, Seung Yeol Cho, Dong Jun Kim, Mi Suk Kim, Hyeun Bum Kim, and Jin Ho Cho
- Subjects
Pig ,Robustness ,Additive ,Stress ,Plant extract ,High stocking density ,Animal culture ,SF1-1100 - Abstract
This study was to investigate effects of different phytogenic feed additives (PFA) in grower finishing pigs with stressed by high stocking density. A total of 84 growing pigs ([Landrace × Yorkshire] × Duroc) with initial body weight (BW) of 28.23 ± 0.21 kg were used for 10 weeks (4 replicate pens with 3 pigs per pen). The dietary treatment consisted of basal diets in animal welfare density (positive control [PC]), basal diet in high stocking density (negative control [NC]), NC + 0.04% bitter citrus extract (PT1), NC + 0.01% microencapsulated blend of thymol & carvacrol (PT2), NC + 0.10% mixture of 40% bitter citrus extract and 10% microencapsulated blend of thymol and carvacrol (PT3), NC + 0.04% premixture of grape seed and grape marc extract, green tea and hops (PT4), and NC + 0.10% fenugreek seed powder (PT5). The reduction of space allowance significantly decreased (p < 0.05) growth performance (average daily gain, average daily feed intake, feed efficiency) and nutrient digestibility (dry matter, crude protein). Also, the fecal score of NC group increased (p < 0.05) compared with other groups. In blood profiles, lymphocyte decreased (p < 0.05), and neutrophil, cortisol, TNF-α increased (p < 0.05) when pigs were in high stocking density. Basic behaviors (feed intake, standing, lying) were inactive (p < 0.05) and singularity behavior (biting) were increased (p < 0.05) under high stocking density. However, PFA groups alleviated the negative effects such as reducing growth performance, nutrient digestibility, increasing stress indicators in blood and animal behavior. In conclusion, PFA groups improved the health of pigs with stressed by high stocking density and PT3 is the most effective.
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- 2024
- Full Text
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28. EMPIRICAL STUDY ON TREATING COCCIDIOSIS IN BROILERS WITH EUCLEA DIVINORUM LEAF EXTRACTS: POULTRY FARM, FEDERAL COLLEGE OF FORESTRY JOS, NIGERIA
- Author
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Anthony C. MGBOJIKWE, Godfrey C. ONUWA, and Oluwafemi M. SALAMI
- Subjects
broilers ,coccidiosis ,diamond-leaved euclea ,photochemistry ,plant extract ,Food processing and manufacture ,TP368-456 - Abstract
The research investigated how the use of a water-based extract from Euclea divinorum leaves can impact the treatment of Coccidiosis in Broiler chickens. One hundred and twenty (120) broilers were experimentally infected with Eimeria tenella and split into 4 categories T1, T2, T3, and T4, each containing three (3) duplicates. Water-based extract of Euclea divinorum was administered at 5%, 10%, 20% and 0% inclusion levels, in treatments T1, T2, T3and T4 (control), respectively. Statistical methods and procedures used in this study include photochemistry, analysis of variance (ANOVA), and descriptive statistics. A study of the chemicals in Euclea divinorum found the existence of alkaloids, flavonoids, saponins, tannins, phenol, glycosides, and a steroid ring. These substances are known for their beneficial healing properties. ANOVA showed a significant difference between different inclusion levels of Euclea divinorum and their effects in the treatment of Coccidiosis. The reported mortality rates for treatments T1, T2, T3, and T4 were monitored and the estimates are 16.7% (5), 10% (3), 56.7% (17) and 66.7% (20), respectively. Thus, Euclea divinorium has great potentials for the treatment and control Coccidiosis in broilers. This study recommends increased cultivation and utilization of medicinal plants, intensive research on the therapeutic effects of plant extracts, subsidized medical supplies and improved extension services.
- Published
- 2024
29. Evaluation of some artificial food preservatives and natural plant extracts as antimicrobial agents for safety
- Author
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Jianrong Shi, Jianhong Xu, Xin Liu, Amira A. Goda, Salah H. Salem, Mohamed M. Deabes, Mohamed I. M. Ibrahim, Khayria Naguib, and Sherif R. Mohamed
- Subjects
Food preservatives ,Plant extract ,Antimicrobial ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Abstract Numerous studies have found that some food preservatives are hazardous to human health and can lead to cancer in the long run. Plant extracts are becoming increasingly popular and garnering the attention of researchers due to their important antibacterial and antioxidant components. In this review, we will focus on some chemical preservatives, the potential health risks of nitrate and nitrite, sodium benzoate, and potassium sorbate in food processing. Natural plant extracts are also discussed as antimicrobials and antioxidants, as well as their potent roles as medicinal plants and antimicrobial agents that can be employed to reduce the percentage of food preservatives. Data reveals that synthetic chemicals in food additives can promote obesity, cancer, asthma, and heart problems. Natural plant extracts have antibacterial and antioxidant characteristics, making them a partially viable alternative to synthetic preservatives. Bioactive compounds, agro-industrial processes, and green processing technologies that are environmentally friendly are important for improving food products.
- Published
- 2024
- Full Text
- View/download PDF
30. Investigations of corrosion inhibition of ethanolic extract of Dillenia suffruticosa leaves as a green corrosion inhibitor of mild steel in hydrochloric acid medium
- Author
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Ukashat Mamudu, Jose Hernandez Santos, Saviour A. Umoren, Mohamad Sahban Alnarabiji, and Ren Chong Lim
- Subjects
Plant extract ,Mild steel ,Acid corrosion ,Corrosion inhibition ,Dillenia suffruticosa ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
The utilization of plant extracts as environmentally friendly corrosion inhibitors has garnered considerable interest in the field of metallic corrosion protection. The primary objective of this study was to explore a new approach for utilizing Dillenia suffruticosa leaves extract (DSLE) to provide corrosion protection for mild steel (MS) in a molar hydrochloric acid solution. The phytocompounds responsible for corrosion prevention were extracted using ethanol with Soxhlet apparatus. The effectiveness of the extract as a corrosion inhibitor was evaluated through gravimetric and electrochemical methods, along with surface analysis of the corroded steel samples. This analysis involved the use of scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FTIR) to examine the specimens with and without addition of the extract as inhibitor. The findings demonstrate that DSLE exhibits notable efficacy as a corrosion inhibitor for MS in an acidic environment. The inhibition efficiency (IE) demonstrates a positive correlation with the concentration of the extract, ultimately reaching a maximum value of 81.4 % at the optimum concentration of 1000 mg/L of the extract investigated. Potentiodynamic polarization data revealed that the DSLE functions as a mixed-type inhibitor.
- Published
- 2024
- Full Text
- View/download PDF
31. Biosynthesis of Zinc Oxide Nanoparticles Using Garlic Peel Extract and Their Antibacterial Potential
- Author
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Ali Abdelmoteleb, Benjamín Valdez-Salas, Ernesto Beltran-Partida, Vianey Mendez-Trujillo, Daniel González-Mendoza, Olivia Tzintzun-Camacho, and Ahmed F. Roumia
- Subjects
zinc nanoparticles ,antimicrobial ,antioxidant ,green synthesis ,plant extract ,Microbiology ,QR1-502 - Abstract
Zinc oxide nanoparticles (ZnO NPs) have gathered interest because of their unique characteristics and potential applications. In the current work, ZnO NPs underwent an eco-friendly biosynthesis process using garlic peel extract. The biosynthesized ZnO NPs were characterized using different analyses including Ultraviolet-visible (UV-vis) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). The produced ZnO NPs exhibited a UV–vis spectrum absorption peak at 365 nm, thus indicating the formation of ZnO NPs. The SEM showed that the biosynthesized ZnO NPs had an irregular surface morphological shape with an average size of 17 nm, according to the DLS analysis. Based on the FTIR findings, the bioactive functional groups responsible for stabilizing and capping the ZnO-NPs were confirmed. The biosynthesized ZnO NPs exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and antimicrobial activities against Gram-positive (Bacillus cereus) and Gram-negative bacteria (Klebsiella pneumonia). Therefore, the plant-mediated biosynthesized ZnNPs can be considered a promising candidate as an antioxidant and antimicrobial agent against pathogenic microbes found in different areas such as food safety and agriculture. Through the utilization of bioinformatics, we identified six potential targets for drug development in K. pneumonia and B. cereus, along with their corresponding interacting residues with zinc oxide nanoparticles. Additionally, our research revealed that the zinc oxide nanoparticles exhibited binding capabilities with the sulfiredoxin domain located at the specific targets of K. pneumonia, a crucial mechanism responsible for the repair of bacterial cells under oxidative stress.
- Published
- 2024
- Full Text
- View/download PDF
32. Use of Conyza canadensis L. Extracts as Biostimulant in Cyclamen persicum Mill.
- Author
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Eunice R. Batista, Andre May, Sergio O. Procópio, Marcia R. Assalin, Helio D. Quevedo, Nicole Binhardi, and Sonia C. N. Queiroz
- Subjects
plant extract ,C. canadensis ,C. persicum ,gas exchanges ,SPAD index ,sustainable agriculture ,Agriculture (General) ,S1-972 ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
Cyclamen (Cyclamen persicum Mill.) is an ornamental plant that is highly susceptible to pathogens, requiring high amounts of phytosanitary products. Therefore, the development of more sustainable alternatives has been required. The present study aimed to analyze the effect of C. canadensis root extract (aqueous and with dichloromethane) applied via foliar or soil, in C. persicum, on gas exchange and the SPAD index and on the biomass of cyclamen. The aqueous extract treatment increased net CO2 assimilation, the transpiration rates, and instantaneous carboxylation efficiency. The water use efficiency values were reduced in the treatments with both extracts. The greatest increases in the SPAD index were provided by the aqueous extract. The cyclamens that received the aqueous extract applied in soil or the dichloromethane extract applied in leaves showed an increase in total biomass and number of leaves. To identify the compounds present in the extracts, CG-MS and LC-MS/MS analyses were performed. The positive effects obtained indicated a high biostimulant effect of C. Canadensis. Thus, the root extracts of C. Canadensis, particularly the aqueous extracts, have the potential to be used to reduce the use of mineral fertilizers and pesticides, promoting agroecological practices and contributing to sustainable agriculture.
- Published
- 2024
- Full Text
- View/download PDF
33. Evaluation of a new plant-based formulation to control Varroa mite (Varroa destructor) in honey bee (Apis mellifera) colonies
- Author
-
Ataollah Rahimi and Shabnam Prichrhreh
- Subjects
apiculture ,colony hygiene ,plant extract ,acaricidal activity ,insecticidal activity ,Veterinary medicine ,SF600-1100 - Abstract
Plant extracts are considered as a suitable alternative to chemical acaricides for the Varroa mite control. Therefore, the present study aimed to investigate the acaricidal and insecticidal activity of extract of Prangos ferulacea plant against Varroa mite and its host in the honey bee colonies in the climatic conditions of Kurdistan province from 2022 to 2024. The P. ferulacea plant was collected in June 2022 from different regions of Kurdistan province in the flowering stage, extracted and then its chemical compounds were identified using GC-MS. The present study was conducted in the form of a factorial experiment based on a completely random basic design with four treatments and seven replications. Before conducting the bioassay experiments, the experimental colonies were homogenized in terms of queen age, population (adults and brood), and honey storage. Also, the initial infestation rate of colonies with Varroa mites was evaluated for adult and brood. The results showed that the extract of 50% of P. ferulacea was relatively favorable acaricidal properties. So that, there was no significant difference with oxalic acid and Apistan treatments in terms of mite mortality percentage in the first time of spraying. Also, the result showed that the bee’s mortality percentage was not more than 7% in any of the times of spraying with the 50% extract of the P. ferulacea, and there was observed a significant difference compared to the oxalic acid and Apistan treatments in terms of bee’s mortality percentage (P
- Published
- 2024
- Full Text
- View/download PDF
34. The Stimulating Effect of Plant Extracts on Tomato Cultivation
- Author
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Zineb Abbad, Fatima-Zahra Rhebbar, Fatima-Zahra Mekaoui, Nor El Houda Tahiri, Rachid Bengueddour, and Lalla Aicha Lrhorfi
- Subjects
plant extract ,stimulant effect ,tomato ,ociumum basilicum ,eucalyptus globulus ,medicinal and aromatic plants ,Ecology ,QH540-549.5 - Abstract
As it might be known, organic products are increasingly in demand. Indeed, one of the most environmentally and friendly innovations are the bio stimulants. Their effect is exposed on the flowering, the plant growth, the fruits and the crop productivity and nutrient use efficiency. In this study we evaluate the effect of two aqueous plant extracts Ocimum basilicum and Eucalyptus globulus on the growth and the performances of tomato. The methodology consist on two parts of evaluation: the effect on the plant performances and the quality of the fruit. At the same time the composition of the two plant extracts have been necessary to evaluate the compound responsible on the effects. The results showed that both aqueous extracts had a positive effect on the plant performances. Indeed, Ocimum basilicum-treated seedling reached 1.35±0.05 cm at the high 50% concentration. Compared with 1.02±0.16 cm for the negative control. Same for Eucalyptus globulus that reached 1.35±0.25 cm at the same dose. For the circumference of the seedlings, the same extract reached 8±0.93cm at the highest concentration. The number of flowers per fruits had a positive effect for two plant extracts. For the quality of the fruit, a positive effect had been evaluated on the fruit weight that reached 121±24g with 8% of Ocimum basilicum and 135±15g with Eucalyptus globulus. Same for circumference; PH; the titrable acidity and the total Carbohydrate. At the same time, it had a negative effect on the total juice that has decreased by the aggregate of the concentrations. It had a neutral effect on the coloration of the fruit that didn’t change by the application of the plants extracts. The phytochemical screening of the plant extracts showed that both Ocimum basilicum and Eucalyptus globulus are high in polyphenols that might be the reason of these positive impacts. This study showed that it is possible to use the plant extracts of Ocimum basilicum and Eucalyptus globulus as biostimulant to have a better production and productivity of tomatoes.
- Published
- 2024
- Full Text
- View/download PDF
35. Effects of Juniperus root extract on wound healing, gene expression, and biochemical response in yellowtail acei Pseudotropheus sp. fish.
- Author
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Önalan, Şükrü and Kankaya, Ertuğrul
- Subjects
- *
PLANT extracts , *CICHLIDS , *GENE expression , *DRUG resistance in bacteria , *HEALING , *WOUND healing - Abstract
Injuries are an undesirable situation in fish kept in aquariums. The use of antibiotics in wound healing is not preferred due to the development of antibiotic resistance in bacteria and for economic reasons. This study was conducted to demonstrate the efficacy of Juniperus root extract in healing experimentally induced incised wounds in yellowtail acei. Wound closure time, morphology, malondialdehyde content, glutathione s-transferase activity, changes in gene expression levels, non-dispersibility of the extract in aqueous environment, and retention on the wound were analyzed. In the antibiotic group, the scarring of the wound was observed on the sixth day. On the other hand, in the root extract group, wound healing was completed on the second day. In addition, a significant decrease in malondialdehyde and glutathione s-transferase levels was observed in the extract group compared to the control group. It was found that SWS2a, RH2B, and LWS gene expression levels started to decrease during wound healing at the end of the second day in the extract-treated group. In the antibiotic group, the same effect was observed on the seventh day. Important properties of the extract used, such as not dispersing in the water environment and remaining on the wound, were also determined. It would be useful to continue the experiments with Juniperus root extract, which has been shown to be effective in wound healing, with microscopic studies on aquaculture and diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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36. The Stimulating Effect of Plant Extracts on Tomato Cultivation.
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Abbad, Zineb, Rhebbar, Fatima-Zahra, Mekaoui, Fatima-Zahra, El Houda Tahiri, Nor, Bengueddour, Rachid, and Lrhorfi, Lalla Aicha
- Subjects
TOMATO farming ,PLANT extracts ,AGRICULTURAL productivity ,FRUIT quality ,BASIL ,EUCALYPTUS globulus - Abstract
As it might be known, organic products are increasingly in demand. Indeed, one of the most environmentally and friendly innovations are the bio stimulants. Their effect is exposed on the flowering, the plant growth, the fruits and the crop productivity and nutrient use efficiency. In this study we evaluate the effect of two aqueous plant extracts Ocimum basilicum and Eucalyptus globulus on the growth and the performances of tomato. The methodology consist on two parts of evaluation: the effect on the plant performances and the quality of the fruit. At the same time the composition of the two plant extracts have been necessary to evaluate the compound responsible on the effects. The results showed that both aqueous extracts had a positive effect on the plant performances. Indeed, Ocimum basilicum-treated seedling reached 1.35 ± 0.05 cm at the high 50% concentration. Compared with 1.02 ± 0.16 cm for the negative control. Same for Eucalyptus globulus that reached 1.35 ± 0.25 cm at the same dose. For the circumference of the seedlings, the same extract reached 8 ± 0.93cm at the highest concentration. The number of flowers per fruits had a positive effect for two plant extracts. For the quality of the fruit, a positive effect had been evaluated on the fruit weight that reached 121 ± 24 g with 8% of Ocimum basilicum and 135 ± 15 g with Eucalyptus globulus. Same for circumference; PH; the titrable acidity and the total Carbohydrate. At the same time, it had a negative effect on the total juice that has decreased by the aggregate of the concentrations. It had a neutral effect on the coloration of the fruit that didn't change by the application of the plants extracts. The phytochemical screening of the plant extracts showed that both Ocimum basilicum and Eucalyptus globulus are high in polyphenols that might be the reason of these positive impacts. This study showed that it is possible to use the plant extracts of Ocimum basilicum and Eucalyptus globulus as biostimulant to have a better production and productivity of tomatoes. [ABSTRACT FROM AUTHOR]
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- 2024
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37. Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review.
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Thomas, Shijith, Gonsalves, Richard A., Jose, Jomy, Zyoud, Samer H., Prasad, Anupama R., and Garvasis, Julia
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- *
BIODEGRADABLE materials , *SUSTAINABLE chemistry , *SILVER nanoparticles , *ENDOENZYMES , *FOOD science , *PLANT extracts - Abstract
The development of an environmentally benign method for the synthesis of nanoparticles has been facilitated by green chemistry. "Green synthesis" uses a range of biological elements like microbes, plants, and other biodegradable materials to produce NPs. Active biomolecules that are secreted by natural strains and present in the plant extracts serve as both reducing and capping/stabilizing agents. Microorganisms' intracellular enzymes can reduce metal ions, which explains how NPs might potentially nucleate. Plant-based synthesis of nanomaterials is particularly promising owing to abundant resources, simplicity of synthesis, and low cost. Silver nanoparticles (AgNPs) are attracting great attention in the research community due to their wide variety of applications in chemistry, food technology, microbiology, and biomedicine. Recent years have seen a large amount of research on the bio-genic synthesis of AgNPs employing biomaterials like plant extract and bacteria as reducing agents. Herein we discuss a thorough overview of the plant-based synthesis of silver nanoparticles (AgNPs), characterization approaches, applications, and toxicity. The review covers the green chemistry and nanotechnology elements of producing AgNPs, including a thorough discussion of the plant extract mediated synthesis, detailed formation mechanism, and a well-balanced emphasis on hazards and advantages. Based on current developments, the optimisation strategies, applications, and interdisciplinary characteristics are also covered in detail. • Plant-based synthesis of nanomaterials is particularly promising. • A comprehensive review on plant-based AgNPs synthesis. • Synthesis, characterization approaches, applications and toxicity are discussed. [ABSTRACT FROM AUTHOR]
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- 2024
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38. Antibacterial, Antioxidant, and Cytotoxic Properties of Eclipta prostrata Extracts.
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Wongkattiya, Nalin, Sanguansermsri, Phanchana, Akawatchai, Chareeporn, Fraser, Ian, and Sanguansermsri, Donruedee
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- *
TREATMENT effectiveness , *CYTOTOXINS , *ASIAN medicine , *DRUG resistance in bacteria , *PLANT extracts - Abstract
Eclipta prostrata is traditionally used in Asian medicine to treat skin diseases. Given the side effects and bacterial resistance of conventional treatments, especially in aging populations, alternative therapies are needed. This study assesses the antibacterial, antioxidant, and cytotoxic properties of E. prostrata extracts, focusing on optimizing extraction and fractionation methods for better efficacy. Dried whole plants were extracted with ethanol. Antibacterial activity was tested against Staphylococcus aureus, MRSA, S. epidermidis, and Pseudomonas aeruginosa using broth microdilution to determine minimum inhibitory concentrations (MIC). Antioxidant activity was assessed using a DPPH radical scavenging assay, and cytotoxicity was tested in Vero cells using an MTT assay. The MIC values for crude ethanol extracts ranged from 1.56 to 3.12 mg/ml, and from 0.78 to 1.56 mg/ml for fractionated compounds, indicating increased antibacterial efficacy in fractionated compounds. Antioxidant assays showed an IC50 of 0.666 mg/ml for crude extracts and 0.174 mg/ml for fractionated compounds, indicating higher antioxidant activity. Total phenolic content was 23.25±2.03 mg GAE/g for crude extracts and 95.56±1.09 mg GAE/g for fractionated compounds. Cytotoxicity assays showed IC50 values of 0.10±0.02 mg/ml for crude extracts and 0.06±0.01 mg/ml for fractionated compounds. This study advances extraction and fractionation methods, yielding extracts with significantly increased antibacterial and antioxidant properties. E. prostrata extracts, particularly fractionated compounds, show promising therapeutic potential, though high cytotoxicity in some fractions requires further investigation. These findings support the potential of E. prostrata in modern pharmaceutical formulations for treating skin conditions, especially those associated with aging. [ABSTRACT FROM AUTHOR]
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- 2024
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39. Enhanced photocatalytic activity of green synthesized zinc oxide nanoparticles using low-cost plant extracts
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Sahar A. Mousa, D. A. Wissa, H. H. Hassan, A. A. Ebnalwaled, and S. A. Khairy
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Plant extract ,ZnO NPs ,Photodegradation ,Oxygen vacancies ,Green synthesis ,Methyl orange (MO) ,Medicine ,Science - Abstract
Abstract Developing stable and highly efficient metal oxide photocatalysts remains a significant challenge in managing organic pollutants. In this study, zinc oxide nanoparticles (ZnO NPs) were successfully synthesized using various plant extracts, pomegranate (P.M), beetroot roots (B.S), and seder, along with a chemical process. The produced ZnO NPs were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet–visible spectroscopy (UV–Vis), Field Emission Scanning Electron Microscope (FESEM), High-Resolution Transmission Electron Microscopy (HRTEM), and Surface Area. For all prepared samples, the results indicated that the composition of the plant extract affects several characteristics of the produced particles, such as their photocatalytic properties, energy bandgap (Eg), particle size, and the ratio of the two intensity (0 0 2) and (1 0 0) crystalline planes. The particle size of the produced NPs varies between 20 and 30 nm. To examine NPs' photocatalytic activity in the presence of UV light, Methyl Orange (MO) was utilized. The Eg of ZnO synthesized by the chemical method was 3.16 e. V, whereas it was 2.84, 2.63, and 2.59 for P.M, Seder, and B.S extracts, respectively. The most effective ZnO NPs, synthesized using Beetroots, exhibited a degradation efficiency of 87 ± 0.5% with a kinetic rate constant of 0.007 min−1. The ratio of the two intensity (0 0 2) and (1 0 0) crystalline planes was also examined to determine a specific orientation in (0 0 2) that is linked to the production of oxygen vacancies in ZnO, which enhances their photocatalytic efficiency. Furthermore, the increase in photocatalytic effectiveness can be attributed to the improved light absorption by the inter-band gap states and effective charge transfer.
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- 2024
- Full Text
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40. Chokeberry (Aronia melanocarpa) as natural antioxidant for the meat industry
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Andrea Mesárošová, Marek Bobko, Lukáš Jurčaga, Alica Bobková, Katarína Poláková, Alžbeta Demianová, Judita Lidiková, Ondřej Bučko, Andrea Mendelová, and Tomáš Tóth
- Subjects
food additive ,lipid oxidation ,meat product ,plant extract ,polyphenols ,Agriculture - Abstract
Aronia melanocarpa is one of the many fruit types rich in polyphenolic compounds. Therefore, we used this fruit in our research as a possible natural antioxidant, which was added to pork sausages. Four groups were prepared: control group, a group with ascorbic acid, and groups with 3 and 5 mL.kg-1 of chokeberry extract. During storage, we monitored changes in pH, colour, texture and oxidative stability of pork sausages and sensory evaluation. We did not observe any negative effects of the extract on the quality of pork sausages during storage. Regarding oxidative stability, the lowest increase in malondialdehyde (MDA) was observed in the group with 5 mL.kg-1 of chokeberry extract, which was comparable to the group with ascorbic acid.
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- 2024
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41. Scutellaria baicalensis Extract Inhibits the UVA-induced Photoaging in NIH3T3 Cells
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Chunmei CAI, Yuye HUANG, Liyan LI, Yue YU, Xiaojuan LIU, Shuxiong XIAO, and Li LU
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scutellaria baicalensis ,anti-aging ,photoaging ,plant extract ,Food processing and manufacture ,TP368-456 - Abstract
Objective: To investigate the protective effect of Scutellaria baicalensis extract on ultraviolet (UVA)-induced photoaging in a fibroblast cell line (NIH3T3). Methods: The cellular photoaging model was established by UVA irradiation, and the protective effects of Scutellaria baicalensis extract on photoaging fibroblasts were investigated using CCK-8 assay, β-galactosidase staining, DAPI staining, and immunofluorescence staining. Results: Scutellaria baicalensis extract significantly promoted cell proliferation and migration of NIH3T3 cells (P
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- 2024
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42. Dietary sanguinarine supplementation recovers the decrease in muscle quality and nutrient composition induced by high-fat diets of grass carp (Ctenopharyngodon idella)
- Author
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Yong Shi, Lei Zhong, Yuanxiang Liu, Shude Xu, Jihong Dai, Yaozhengtai Zhang, and Yi Hu
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Plant extract ,Muscle feature ,Myofiber characteristics ,Muscular texture ,Protein metabolism ,Animal culture ,SF1-1100 - Abstract
The intake of high-fat diets (HFD) has been shown to diminish the muscle quality of aquatic animals. Sanguinarine, as an excellent additive, exhibits the capability to reduce fat deposition and alleviate inflammation. However, its role in the muscle quality reduction caused by HFD remains unclear. An eight-week trial was conducted to investigate the impacts of dietary supplementation of sanguinarine at 1200 μg/kg (HFDS; crude fat = 10%) on the muscle quality of grass carp (Ctenopharyngodon idellus) in comparison to a basic diet (CON, crude fat = 5%). Each group had 3 replicates, with 40 fish per replicate. This experiment employed one-way ANOVA and Duncan's multiple comparisons of the means. The results showed that the HFD exhibited lower growth performance, reduced protein deposition, myofiber diameter, and muscle hardness, coupled with higher levels of fat deposition and inflammation when compared with the CON. However, HFDS improved growth performance (P < 0.05), fat metabolism (ppar-α ( P = 0.001), lpl (P < 0.001), atgl (P < 0.001), and cpt1 (P = 0.001) expression exhibited a significant elevation), protein deposition (the protein and mRNA levels of AKT (P = 0.004), PI3K (P = 0.027), TOR (P = 0.005), and P70S6K (P = 0.007) demonstrated a marked increase), myofiber diameter, muscle hardness, and the total content of eicosapentaenoic acid and docosahexaenoic acid. Furthermore, the HFDS reduced oxidative damage caused by fat deposition by significantly downregulating nf-κb (P < 0.001), il-1β (P < 0.001), il-6 (P < 0.001), il-8 (P = 0.003), and tnf-α (P < 0.001) expression and markedly upregulated nrf2 (P < 0.001), gpx4 (P < 0.001), cat (P < 0.001), sod (P < 0.001), and gr (P = 0.003) expression. The findings from this study suggest that sanguinarine has the potential to alleviate the adverse effects of HFD on growth and muscle quality, providing a theoretical foundation for its practical implementation.
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- 2024
- Full Text
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43. Enhanced photocatalytic activity of green synthesized zinc oxide nanoparticles using low-cost plant extracts.
- Author
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Mousa, Sahar A., Wissa, D. A., Hassan, H. H., Ebnalwaled, A. A., and Khairy, S. A.
- Subjects
- *
PLANT extracts , *PHOTOCATALYSTS , *FIELD emission electron microscopes , *CHEMICAL processes , *ZINC oxide , *ULTRAVIOLET-visible spectroscopy - Abstract
Developing stable and highly efficient metal oxide photocatalysts remains a significant challenge in managing organic pollutants. In this study, zinc oxide nanoparticles (ZnO NPs) were successfully synthesized using various plant extracts, pomegranate (P.M), beetroot roots (B.S), and seder, along with a chemical process. The produced ZnO NPs were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet–visible spectroscopy (UV–Vis), Field Emission Scanning Electron Microscope (FESEM), High-Resolution Transmission Electron Microscopy (HRTEM), and Surface Area. For all prepared samples, the results indicated that the composition of the plant extract affects several characteristics of the produced particles, such as their photocatalytic properties, energy bandgap (Eg), particle size, and the ratio of the two intensity (0 0 2) and (1 0 0) crystalline planes. The particle size of the produced NPs varies between 20 and 30 nm. To examine NPs' photocatalytic activity in the presence of UV light, Methyl Orange (MO) was utilized. The Eg of ZnO synthesized by the chemical method was 3.16 e. V, whereas it was 2.84, 2.63, and 2.59 for P.M, Seder, and B.S extracts, respectively. The most effective ZnO NPs, synthesized using Beetroots, exhibited a degradation efficiency of 87 ± 0.5% with a kinetic rate constant of 0.007 min−1. The ratio of the two intensity (0 0 2) and (1 0 0) crystalline planes was also examined to determine a specific orientation in (0 0 2) that is linked to the production of oxygen vacancies in ZnO, which enhances their photocatalytic efficiency. Furthermore, the increase in photocatalytic effectiveness can be attributed to the improved light absorption by the inter-band gap states and effective charge transfer. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil).
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Vongnhay, Vanessa, Shukla, Mukund R., Ayyanath, Murali-Mohan, Sriskantharajah, Karthika, and Saxena, Praveen K.
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PLANT regulators ,REGENERATION (Botany) ,OCIMUM sanctum ,GROWTH regulators ,ABSCISIC acid ,SOMATIC embryogenesis - Abstract
Plant growth regulators (PGRs) play a vital role in the induction of morphogenesis in vitro. Synthetic PGRs are commonly used to induce organogenesis and somatic embryogenesis from various explants, while natural substances are rarely utilized. This study aimed to enhance the regenerative response in Nicotiana tabacum leaf explants using Tulsi (Ocimum sanctum) leaf extract and to elucidate the biochemical interactions during modulation of endogenous plant growth regulators, including indole-3-acetic acid (IAA), abscisic acid (ABA), zeatin, and 6-(γ, γ-dimethylallylamino) purine (2iP). Tulsi leaf extract significantly improved shoot production through interactions between endogenous hormones and those present in the extract, which enhanced stress mitigation. The 20% Tulsi leaf extract treatment produced significantly more shoots than the control, coinciding with increased endogenous IAA and zeatin levels starting on day 10 in culture. Furthermore, ABA and zeatin concentrations increased on days 15 and 25, respectively, in the 20% Tulsi extract treatment, suggesting their role in the induction of somatic embryo-like structures. ABA likely acts as an activator of stress responses, encouraging the development of these structures. Additionally, 2iP was involved in the induction of both forms of regeneration in the 10% and 20% extract treatments, especially in combination with ABA. These results suggest that Tulsi leaf extract holds promising potential as a natural supplement for increasing plant regeneration in vitro and advancing our understanding of how natural extracts of plant origin can be harnessed to optimize plant regeneration processes in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
45. Effects of various extract drinks on endurance in long-distance running training.
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Yu, Zhihua and Sun, Zehao
- Subjects
- *
LONG-distance runners , *BLOOD lactate , *PLANT extracts , *TRACK & field , *ACANTHOPANAX , *LONG-distance running , *SPORTS drinks - Abstract
This paper briefly introduces plant extracts and their combinations with sports drinks. The research involved 40 sportsmen who are affiliated with the Track and Field Department of the School of Physical Education in South-Central Minzu University. This paper aimed to explore the influence of sports drinks enhanced with extracts of Codonopsis pilosula and Acanthopanax senticosus on the endurance levels of long-distance runners. The participants were divided into four groups: a placebo group, a group receiving only C. pilosula , a group receiving A. senticosus , and a composite group. Endurance performance indices for long-distance running were evaluated both before and after the training. Before training, the heart rate, blood lactate level, and maximum oxygen uptake of the placebo group were 159.53 ± 2.21 times/min, 9.7 ± 1.2 mmol/L, and 66.18 ± 2.35 mL/kg/min, respectively; the single Codonopsis pilosula group was 158.33 ± 2.24 times/min, 9.6 ± 1.1 mmol/L, and 65.87 ± 1.88 mL/kg/min, respectively; the single Acanthopanax senticosus group was 159.66 ± 3.67 times/min, 9.7 ±1.0 mmol/L, and 66.32 ± 1.47 mL/kg/min, respectively; the Codonopsis pilosulae and Acanthopanax senticosus composite group was 158.21 ± 1.28 times/min, 9.6 ± 0.9 mmol/L, and 66.87 ± 1.12 mL/kg/min, respectively. After training, the corresponding values of the placebo group were 162.53 ± 3.21 times/min, 9.6 ± 0.8 mmol/L, and 68.85 ± 3.25 mL/kg/min, respectively; the single Codonopsis pilosula group was 145.33 ± 2.25 times/min, 9.1 ± 0.5 mmol/L, and 73.69 ± 2.58 mL/kg/min, respectively; the single Acanthopanax senticosus group was 142.66 ± 3.69 times/min, 9.1 ± 0.8 mmol/L, and 73.84 ± 2.19 mL/kg/min, respectively; the composite group was 131.21 ± 1.36 times/min, 8.3 ± 0.3 mmol/L, and 77.19 ± 3.14 mL/kg/min, respectively. Sports drinks that include extracts of C. pilosula and A. senticosus significantly increased endurance levels. The concurrent use of C. pilosula and A. senticosus was more beneficial in enhancing endurance performance for long-distance running in comparison to using each extract individually. [ABSTRACT FROM AUTHOR]
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- 2024
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46. SYNTHESIS OF ACRYLIC ACID-BASED ZINC OXIDE NANOCOMPOSITE WITH ANTICANCER APPLICATION.
- Author
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Subashini, K., Chitra, G., and Sujatha, V.
- Subjects
- *
PLANT polymers , *POLYMERIC nanocomposites , *NANOPARTICLE size , *PLANT extracts , *NANOPARTICLES - Abstract
The zinc oxide nanoparticles were synthesized using Brassia actinophylla (B. actinophylla)flower extract. The ZnO nanoparticles were introduced into TDA hydrogel (T-tartaric acid, D-diethylene glycol, A-acrylic acid) to form the TDA-ZnO nanocomposite. The UV-peak was observed at 280 nm and the FT-IR peak was obtained at 685 cm-1 confirming the presence of nanoparticles in the hydrogel. The nanoparticle's size was confirmed by TEM and SEM analysis. The anticancer activity of polymer nanocomposites was performed against the A549 lung cancer cell line and the results have been recorded and compared. [ABSTRACT FROM AUTHOR]
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- 2024
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- View/download PDF
47. Evaluation of Antimicrobial Property and Phytochemicals of Local Phyllanthus niruri Plant.
- Author
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Ghazali, Muhammad Yusri, Tarmizi, Nur Hazirah, and Yusof, Hartini
- Subjects
- *
CARIOGENIC agents , *DISC diffusion tests (Microbiology) , *ESCHERICHIA coli , *PHYLLANTHUS , *GRAM-negative bacteria , *GRAM-positive bacteria , *CUTIBACTERIUM acnes - Abstract
Introduction: Employing natural plants as antibacterial agents is one strategy for combating the challenges of drug-resistant microorganisms. This research examines the antibacterial activity of methanolic Phyllanthus niruri (P. niruri) plant extract against Propionibacterium acnes (P. acnes), Staphylococcus epidermidis (S. epidermidis), Streptococcus mutans (S. mutans), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), as well as its phytochemical components. Materials and methods: The antibacterial activity was evaluated utilising agar well diffusion, broth microdilution, and the streaking technique. The phytochemical compounds were identified via qualitative testing. Results: Antimicrobial susceptibility testing (AST) revealed that the mean inhibition zone ranged between 48.00 mm and 9.33 mm. P. acnes had the highest level of inhibition (48.00±1.00 mm), whereas E. coli had the lowest (9.33±0.58 mm). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) suggested a concentration range between 1.95 mg/ml and 125 mg/ml. The extract's antibacterial activity indicated that gram-positive bacteria were more sensitive than gram-negative bacteria. Additionally, the plant extract contained alkaloids, flavonoids, glycosides, phenolics, tannins, saponins, and steroids. Conclusion: The findings of this investigation indicate that P. niruri extract might serve as a source of antibacterial agent in the development of alternative antibiotic medication. [ABSTRACT FROM AUTHOR]
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- 2024
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48. Efficient reduction of Cr(VI) by guava (Psidium guajava) leaf extract and its mitigation effect on Cr toxicity in rice seedlings.
- Author
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Ran, Maodi, Lu, Yongqing, Ren, Yanzhen, He, Li, and Li, Jiaokun
- Subjects
- *
GUAVA , *X-ray photoelectron spectroscopy , *RICE , *HEXAVALENT chromium , *GALLIC acid , *CROP yields - Abstract
• AEP acting as an effective Cr(VI) reducing agent. • Cr(VI) toxicity in rice was significantly alleviated with AEP application. • AEP promoted Cr adsorption to the root cell walls and limited the Cr transport. • AEP application actively triggered the expression of Cr defense-related genes in rice. Hexavalent chromium (Cr(VI)) is a toxic element that has negative impacts on crop growth and yield. Using plant extracts to convert toxic Cr(VI) into less toxic Cr(III) may be a more favorable option compared to chemical reducing agents. In this study, the potential effects and mechanisms of using an aqueous extract of Psidium guajava L. leaves (AEP) in reducing Cr(VI) toxicity in rice were comprehensively studied. Firstly, the reducing power of AEP for Cr(VI) was confirmed by the cyclic voltammetry combined with X-ray photoelectron spectroscopy (XPS) assays. The highest Cr(VI) reduction efficiency reached approximately 78% under 1.5 mg gallic acid equivalent (GAE)/mL of AEP and 10 mg/L Cr(VI) condition. Additionally, Cr(VI) stress had a significant inhibitory effect on rice growth. However, the exogenous application of AEP alleviated the growth inhibition and oxidative damage of rice under Cr(VI) stress by increasing the activity and level of enzymatic and non-enzymatic antioxidants. Furthermore, the addition of AEP restored the ultrastructure of root cells, promoted Cr adsorption onto root cell walls, and limited the translocation Cr to shoots. In shoots, AEP application also triggered the expression of specific genes involved in Cr defense and detoxification response, including photosynthesis pathways, antioxidant systems, flavonoids biosynthesis, and plant hormone signal transduction. These results suggest that AEP is an efficient reduction agent for Cr(VI), and exogenous application of AEP may be a promising strategy to mitigate the harm of Cr(VI) on rice, ultimately contributing to improved crop yield in Cr-contaminated environments. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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49. Antifungal properties of Cymbopogon citratus (DC.) Stapf—A scoping review.
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Falcon, Robbi Miguel G., Fahrenbach, Serina U., Feliciano, Jeremiah F., Flores, Bea Micah B., Dida-Agun, Aisha S., Domingo, Eldrin Jalen V., Domingo, Francis Kiro S., Duran, Helene Elise T., Dungala, Darryl B., Dychiao, Garret Royce K., Evangelista, Paul Emmanuel D., Facon, Haggai Elisha L., FlorCruz, Francis Elmo R., Florita, Mary Hazel B., Giron, Maria Stella T., and Yabes, Ailyn M.
- Subjects
- *
LEMONGRASS , *ANTIFUNGAL agents , *GAS chromatography/Mass spectrometry (GC-MS) , *PLANT extracts , *CANDIDA albicans , *ESSENTIAL oils - Abstract
Cymbopogon citratus (DC.) Stapf is an abundant medicinal plant, typically utilized as an antifungal herbal remedy. Despite being widely utilized for its health benefits, to our knowledge, there are no comprehensive literature reviews available on the antifungal properties of the plant. In this review, we aim to synthesize current studies on the antifungal properties of C. citratus plant extracts in order to determine the depth of research available while identifying literature gaps, to help provide focus for future investigations. We conducted a systematic search of the databases, Web of Science, MEDLINE, and EBSCO CINAHL, for studies and a thorough scan of relevant grey literature on the topic. Data on plant part utilized, type of extract and extraction method, test organism used, antifungal assay used, reported MIC or MFC, as well as the proposed mechanism of antifungal action, literature gaps, and conclusions were identified and described. Studies were screened for eligibility and data were extracted, charted, and summarized narratively. A total of 117 articles from inception to October 15, 2023 were screened, and 27 were eligible for inclusion, covering the years 1993 to 2023. Results showed the most utilized plant part of C. citratus was the leaves (55 %) and extracts were obtained mostly through hydro-distillation (33 %). Citrals were the most common chemical constituent and were characterized most frequently using Gas Chromatography-Mass Spectrometry (GC–MS). The most common test organism used was Candida albicans (55 %), with the antifungal effect tested with diffusion assays (52 %), followed by broth microdilution methods (41 %). The estimated mean for MIC of C. citratus was 1.42 mg/mL and 0.57 mg/mL for the MFC across various species. Most studies suggested that C. citratus specifically targets and disrupts the cell membrane and cell wall integrity by inhibiting specific biosynthetic pathways. In conclusion, C. citratus is a promising source for antifungal herbal remedies. However, additional studies are necessary to identify the specific bioactive metabolites responsible for the antifungal mechanism of action and to ascertain the overall effectiveness and safety of the extract for clinical use. [Display omitted] • Lemongrass leaf essential oil extracted via hydro-distillation was the most utilized. • Candida albicans was the most common test organism, tested via broth microdilution. • The cell wall and membrane are the most likely antifungal targets. • The MIC and MFC for C. albicans was 1.42 mg/mL and 0.57 mg/mL. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
- View/download PDF
50. Effect of dietary Lagnaria breviflora leaves extract on the growth performance, haematological, antioxidative and immune responses of African catfish (Clarias gariepinus) juveniles.
- Author
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Paray, Bilal Ahamad, Adeshina, Ibrahim, Bhat, Eijaz Ahmed, Raji, Abdullateef, Umma, Samuel B., Alliy, Robiat O., and Tiamiyu, Lateef O.
- Subjects
- *
LEUKOCYTE count , *CLARIAS gariepinus , *DISEASE resistance of plants , *AEROMONAS hydrophila , *FISH feeds , *WEIGHT gain , *LYSOZYMES - Abstract
The effects of dietary Lagnaria breviflora leaves extract (LBLE) on the growth performance, feed utilisation and haematological parameters of juveniles African catfish, Clarias gariepinus (mean = 8.45 ± 0.6 g) raised in a flow water (mean temperature 26.7°C, mean pH 7.52, mean dissolved oxygen 6.05 mg/L) were assessed in this study. Diets (400 g/kg crude protein; 4631.5 kcal/kg gross energy) supplemented with LBLE at levels of 0 (control), 0.5, 1.0, 1.5 or 2.0 g/kg diet were served to fish in triplicates (each replicate has 20 fish) until they appeared satiated for 12 weeks. Then, they were challenged with Aeromonas hydrophila infection for further 2 weeks. Fish served dietary LBLE had considerable higher weight gain (64.76 g) and specific growth rate (765.5% g/day) than the fish group fed with the control diet with a reduced feed conversion ratio (1.45) (p < 0.05). Compared to fish fed with the control diet, the villi height (146.30 µm) and width (284.35 µm) of the intestines increased greatly in a dose (LBLE in diets) dependent order. Similarly, dietary LBLE increased (p < 0.05) the packed cell volume (46.11%) and haemoglobin (17.03 g/dL), whereas 1.5 g of LBLE increased the counts of white blood cells. Glutathione S‐transferase (769.43 U/mg protein), glutathione peroxidase (84.14 U/mg protein) and superoxide dismutase (433.15 U/mg protein) activities were significantly higher (p < 0.05) in fish fed with diets supplemented with LBLE compared to the control one. Additionally, dietary LBLE increased phagocytic and lysozyme activities and protected C. gariepinus against bacterial infections where the lowest death was observed in the fish fed on the diet containing 1.5 g LBLE/kg feed. These findings showed that the fish fed with LBLE/kg diet improved their immune system, antioxidant and growth performance in addition to providing protection from A. hydrophila infection with the optimum dose of 1.80 g/kg diet. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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