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2 results on '"Plants, Genetically Modified - genetics, physiology"'

1. Phytochrome-controlled expression of a wheat Cab gene in transgenic tobacco seedlings

Author

UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries, Nagy, F, Kay, S A, Boutry, Marc, Hsu, M Y, Chua, N H, UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries, Nagy, F, Kay, S A, Boutry, Marc, Hsu, M Y, and Chua, N H

Abstract

We have monitored changes in the chlorophyll a/b-binding protein (Cab) mRNA levels in etiolated wheat leaves exposed to light of different wavelengths by Northern blot hybridizations and 5' S1 nuclease protection assays. Accumulation of the Cab mRNA and the specific Cab-1 transcript is regulated by phytochrome. Transcript levels are elevated by red light and the red enhancement can be partially reversed by far-red light. Moreover, far-red light alone can elicit a small increase in the transcript levels. These characteristic features of the wheat Cab-1 phytochrome response can be recapitulated in etiolated seedlings of transgenic tobacco containing the Cab-1 gene. Analyses of a chimeric construct revealed that a 1.8-kb 5'-flanking fragment (-1816 to +31) of the Cab-1 gene can confer phytochrome response as well as leaf-specific expression on a heterologous coding sequence.

Published
1986

2. Phytochrome-controlled expression of a wheat Cab gene in transgenic tobacco seedlings

Author

Steve A. Kay, Nam-Hai Chua, Mei-Yin Hsu, Marc Boutry, Ferenc Nagy, and UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries

Subjects
Nicotiana tabacum, Light-Harvesting Protein Complexes, Light-Harvesting Protein Complexes - biosynthesis, genetics, Chimeric gene, General Biochemistry, Genetics and Molecular Biology, RNA, Messenger - genetics, Gene Expression Regulation, Plant, Phytochrome - physiology, Tobacco, Gene expression, Seedling - genetics, physiology, RNA, Messenger, Northern blot, Molecular Biology, Gene, Triticum, QH506, Genetics, General Immunology and Microbiology, Phytochrome, biology, Gene Expression Regulation, Plant - physiology, Tobacco - physiology, General Neuroscience, Genetic transfer, fungi, food and beverages, Articles, Triticum - genetics, Plants, Genetically Modified, biology.organism_classification, Blotting, Northern, Molecular biology, Seedlings, Etiolation, Plants, Genetically Modified - genetics, physiology
Abstract

We have monitored changes in the chlorophyll a/b-binding protein (Cab) mRNA levels in etiolated wheat leaves exposed to light of different wavelengths by Northern blot hybridizations and 5' S1 nuclease protection assays. Accumulation of the Cab mRNA and the specific Cab-1 transcript is regulated by phytochrome. Transcript levels are elevated by red light and the red enhancement can be partially reversed by far-red light. Moreover, far-red light alone can elicit a small increase in the transcript levels. These characteristic features of the wheat Cab-1 phytochrome response can be recapitulated in etiolated seedlings of transgenic tobacco containing the Cab-1 gene. Analyses of a chimeric construct revealed that a 1.8-kb 5'-flanking fragment (−1816 to +31) of the Cab-1 gene can confer phytochrome response as well as leaf-specific expression on a heterologous coding sequence.

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