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Pherosphaera fitzgeraldii , the Dwarf Mountain Pine, is a critically endangered conifer restricted to cliff ledges within waterfall spray zones in the Greater Blue Mountains Area of New South Wales, Australia. This species is under threat from pollution, invasive weeds, and limited recruitment, which are contributing to its declining population. Using a comprehensive conservation genomic approach, we assessed the population health of P. fitzgeraldii and developed strategic recommendations for its management. Genomic analysis of P. fitzgeraldii revealed the presence of two distinct genetic groups despite the limited distribution of the species. This genetic structure aligns with prevailing wind currents, indicating potential restrictions to pollen or seed exchange between subpopulations. Furthermore, limited gene flow was observed even among nearby subpopulations, emphasizing the risk of increased genetic differentiation due to ongoing isolation. Clonality is widespread in some subpopulations, and all subpopulations displayed signs of inbreeding and genetic depletion despite subdioecy, indicative of historical constraints on gene flow and small population sizes. We offer genomic-based recommendations for prioritizing conservation sites, enhancing genetic diversity in ex situ collections, and guiding future management. Further studies to uncover the sex determination mechanism of P. fitzgeraldii could help maintain balanced sex ratios in ex situ collections and support future genetic rescue efforts. This study underscores the value of genomics in informing protection and recovery of unique threatened species. [ABSTRACT FROM AUTHOR]

Pherosphaera fitzgeraldii, the Dwarf Mountain Pine, is a critically endangered conifer restricted to cliff ledges within waterfall spray zones in the Greater Blue Mountains Area of New South Wales, Australia. This species is under threat from pollution, invasive weeds, and limited recruitment, which are contributing to its declining population. Using a comprehensive conservation genomic approach, we assessed the population health of P. fitzgeraldii and developed strategic recommendations for its management.Genomic analysis of P. fitzgeraldii revealed the presence of two distinct genetic groups despite the limited distribution of the species. This genetic structure aligns with prevailing wind currents, indicating potential restrictions to pollen or seed exchange between subpopulations. Furthermore, limited gene flow was observed even among nearby subpopulations, emphasizing the risk of increased genetic differentiation due to ongoing isolation. Clonality is widespread in some subpopulations, and all subpopulations displayed signs of inbreeding and genetic depletion despite subdioecy, indicative of historical constraints on gene flow and small population sizes.We offer genomic-based recommendations for prioritizing conservation sites, enhancing genetic diversity in ex situ collections, and guiding future management. Further studies to uncover the sex determination mechanism of P. fitzgeraldii could help maintain balanced sex ratios in ex situ collections and support future genetic rescue efforts. This study underscores the value of genomics in informing protection and recovery of unique threatened species.

We describe a new genus of Leliacladus Batista & L.Kunzmann with the type species Leliacladus (Brachyphyllum) castilhoi (Duarte, 1985) Batista & L.Kunzmann gen. nov. et comb. nov., a rare fossil conifer that has been described from the Aptian (Lower Cretaceous) Romualdo Formation of the Araripe Basin, northeastern Brazil. We decided to leave this new fossil-genus unasigned to any existing family as it does not display sufficient number of anatomical characters. We re-studied the type material of the type species as well as we described additional new material from the Aptian Crato Formation of the same basin. The more recently found fossil materials include large portions of leafy shoots excavated from laminated lacustrine limestones. In contrast to the type material, the new material shows the replacement of organic matter by iron oxide, which is suitable for investigating anatomical features of the wood and leaves. The material allowed for a reappraisal of the systematic position of the fossil plant. Together, the morphological and anatomical characters revealed sufficient evidence to separate the conifer from Brachyphyllum and accommodate it in a new fossil-genus. Leliacladus gen. nov. is defined by the presence of comparatively thick and short claviform lateral branches, and the absence of thinner ultimate order branches with gradual tapering axes. The wood is characterized by dense tracheids with uniseriate pitting in the radial walls and cross-fields that possess one or two large pits. The rays are one to three cells high. The epidermises of the densely and helically arranged scale leaves show non-papillate cyclocytic stomatal complexes slightly sunken that are mostly scattered and randomly arranged across the abaxial surface. This combination of characters suggests the material belongs to the conifer families Podocarpaceae or Cheirolepidiaceae. The arrangement of the sparsely branched, but woody shoots of Leliacladus castilhoi gen. nov. et comb. nov. suggest a hypothetical candelabra-like growth habit of the plant, and the remarkable thickness of the axes suggests a hypothetical xeromorphic adaptation to the semiarid paleoenvironment.

Premise: The Cenozoic Macquarie Harbour Formation (MHF) hosts one of the oldest and southernmost post‐Cretaceous fossil plant assemblages in Australia. Coinciding with the Early Eocene Climatic Optimum (EECO) and predating the breakup of Australia from Antarctica, it offers critical data to study the diversity and extent of the Austral Polar Forest Biome, and the floristic divergence between Australasia and South America resulting from the Gondwana breakup. Methods: The micromorphology and macromorphology of new fossil plant compressions from the MHF were described and systematically analyzed. Previously published non‐flowering plant records were reviewed and revised. Macrofossil abundance data were provided. The flora was compared with other early Paleogene assemblages from across the Southern Hemisphere. Results: Twelve species of non‐flowering plants were identified from the macrofossil record. Conifers include Araucariaceae (Araucaria macrophylla, A. readiae, A. timkarikensis sp. nov., and Araucarioides linearis), Podocarpaceae (Acmopyle glabra, Dacrycarpus mucronatus, Podocarpus paralungatikensis sp. nov., and Retrophyllum sp.), and Cupressaceae (Libocedrus microformis). Dacrycarpus linifolius was designated a junior synonym of D. mucronatus. Further components include a cycad (Bowenia johnsonii, Zamiaceae), a pteridosperm (Komlopteris cenozoicus, Umkomasiaceae), and a fern (Lygodium dinmorphyllum, Schizaeaceae). Conclusions: The fossil assemblage represents a mixed near‐polar forest with a high diversity of conifers. The morphology and preservation of several species indicate adaptations to life at high latitudes. The coexistence of large‐ and small‐leaved conifers implies complex, possibly open forest structures. Comparisons with contemporaneous assemblages from Argentina support a circumpolar biome during the EECO, reaching from southern Australia across Antarctica to southern South America. [ABSTRACT FROM AUTHOR]

We describe a new genus of Leliacladus Batista & L.Kunzmann with the type species Leliacladus (Brachyphyllum) castilhoi (Duarte, 1985) Batista & L.Kunzmann gen. nov. et comb. nov., a rare fossil conifer that has been described from the Aptian (Lower Cretaceous) Romualdo Formation of the Araripe Basin, northeastern Brazil. We decided to leave this new fossil-genus unasigned to any existing family as it does not display sufficient number of anatomical characters. We re-studied the type material of the type species as well as we described additional new material from the Aptian Crato Formation of the same basin. The more recently found fossil materials include large portions of leafy shoots excavated from laminated lacustrine limestones. In contrast to the type material, the new material shows the replacement of organic matter by iron oxide, which is suitable for investigating anatomical features of the wood and leaves. The material allowed for a reappraisal of the systematic position of the fossil plant. Together, the morphological and anatomical characters revealed sufficient evidence to separate the conifer from Brachyphyllum and accommodate it in a new fossil-genus. Leliacladus gen. nov. is defined by the presence of comparatively thick and short claviform lateral branches, and the absence of thinner ultimate order branches with gradual tapering axes. The wood is characterized by dense tracheids with uniseriate pitting in the radial walls and cross-fields that possess one or two large pits. The rays are one to three cells high. The epidermises of the densely and helically arranged scale leaves show non-papillate cyclocytic stomatal complexes slightly sunken that are mostly scattered and randomly arranged across the abaxial surface. This combination of characters suggests the material belongs to the conifer families Podocarpaceae or Cheirolepidiaceae. The arrangement of the sparsely branched, but woody shoots of Leliacladus castilhoi gen. nov. et comb. nov. suggest a hypothetical candelabra-like growth habit of the plant, and the remarkable thickness of the axes suggests a hypothetical xeromorphic adaptation to the semiarid paleoenvironment. [ABSTRACT FROM AUTHOR]

We describe a new genus of Leliacladus Batista & L.Kunzmann with the type species Leliacladus (Brachyphyllum) castilhoi (Duarte, 1985) Batista & L.Kunzmann gen. nov. et comb. nov., a rare fossil conifer that has been described from the Aptian (Lower Cretaceous) Romualdo Formation of the Araripe Basin, northeastern Brazil. We decided to leave this new fossil-genus unasigned to any existing family as it does not display sufficient number of anatomical characters. We re-studied the type material of the type species as well as we described additional new material from the Aptian Crato Formation of the same basin. The more recently found fossil materials include large portions of leafy shoots excavated from laminated lacustrine limestones. In contrast to the type material, the new material shows the replacement of organic matter by iron oxide, which is suitable for investigating anatomical features of the wood and leaves. The material allowed for a reappraisal of the systematic position of the fossil plant. Together, the morphological and anatomical characters revealed sufficient evidence to separate the conifer from Brachyphyllum and accommodate it in a new fossil-genus. Leliacladus gen. nov. is defined by the presence of comparatively thick and short claviform lateral branches, and the absence of thinner ultimate order branches with gradual tapering axes. The wood is characterized by dense tracheids with uniseriate pitting in the radial walls and cross-fields that possess one or two large pits. The rays are one to three cells high. The epidermises of the densely and helically arranged scale leaves show non-papillate cyclocytic stomatal complexes slightly sunken that are mostly scattered and randomly arranged across the abaxial surface. This combination of characters suggests the material belongs to the conifer families Podocarpaceae or Cheirolepidiaceae. The arrangement of the sparsely branched, but woody shoots of Leliacladus castilhoi gen. nov. et comb. nov. suggest a hypothetical candelabra-like growth habit of the plant, and the remarkable thickness of the axes suggests a hypothetical xeromorphic adaptation to the semiarid paleoenvironment. [ABSTRACT FROM AUTHOR]

Antecedentes: Sinaloa es uno de los estados con menor número de colectas botánicas en México, pero con mayor número de observaciones en la plataforma iNaturalist/iNaturalistMX. En 2021 se publicó el listado florístico del estado, pero desde entonces se han realizado nuevas exploraciones y descubrimientos. Preguntas: ¿Cuántas y cuáles especies de plantas vasculares deben añadirse al listado de Sinaloa? ¿Qué fuentes de información las sustentan? Especies de estudio: Plantas vasculares. Sitio y años de estudio: Sinaloa, México (2021-2024). Métodos: Se revisó literatura, herbarios, repositorios digitales, iNaturalistMX y se ejecutaron exploraciones botánicas. Se realizaron análisis de riqueza para las especies añadidas mediante exploraciones recientes y se compararon con las bases de datos de colecciones y iNaturalistMX. Resultados: Se añadieron 130 especies a la flora de Sinaloa, incluyendo tres familias (Podocarpaceae, Simaroubaceae y Symplocaceae) y 18 géneros. Podocarpaceae y Simaroubaceae se registran por primera vez para la Sierra Madre Occidental. Las principales fuentes de información fueron iNaturalistMX, colectas recientes y omisiones encontradas en la literatura. Noventa y dos de las 130 adiciones cuentan con al menos un registro en iNaturalistMX, de las cuales 31 son registros únicos. Las áreas con más especies añadidas coinciden con regiones poco representadas en las colecciones, históricamente consideradas de baja riqueza por sesgos de colectas. Conclusiones: La flora de Sinaloa está lejos de conocerse completamente y los sesgos de colectas han producido interpretaciones erróneas en los patrones de su riqueza. El uso de iNaturalistMX y exploraciones recientes permitieron identificar nuevas áreas ricas en biodiversidad.

Two undescribed dissymmetric bis -diterpenes, annamiacids A (1) and B (2), were isolated from the renewable twigs and leaves of Podocarpus annamiensis, a 'vulnerable' conifer endemic to China. Their structures and absolute configurations were determined by spectroscopic and computational methods. The homodimeric (abietane- O -abietane, 1) and heterodimeric (abietane- O -totarane, 2) dimers were constructed from an identical phenolic abietane unit through an ether linkage with a phenolic abietane acid and a phenolic totarane acid, respectively. Both comprise a rare intramolecular 6-ketal-19,6- γ -lactone motif. Compound 2 could significantly inhibit ATP-citrate lyase with an IC 50 value of 5.92 μ M. A molecular docking study disclosed alternative H-bonds/interacting amino acid residues and binding pockets between 2 and the ACL enzyme when compared with those of compound 1 , which supports their in vitro data. [Display omitted] • The endangered conifer Podocarpus annamiensis has been phytochemically studied. • Two bis -diterpenes containing a rare ketal- γ -lactone motif were identified. • Heterodimeric abietane- O -totarane (2) could significantly inhibit the ACL enzyme. • A molecular docking study disclosed the in vitro inhibitory disparity of 1 and 2. [ABSTRACT FROM AUTHOR]

Summary: The mid-Cretaceous is an important time for the diversification of forests globally, including the rise to dominance of the angiosperms and the beginning of the isolation of Zealandia. In New Zealand, little information is available on the mid-Cretaceous xyloflora. New specimens of fossil wood from the mid-Cretaceous Tupuangi Formation were collected from Waihere Bay, Pitt Island, Chatham Islands, of which 16 well-preserved samples were identified, representing Araucariaceae (Agathoxylon , 5 samples), Cupressaceae (Taxodioxylon and Cupressinoxylon , one sample each), Podocarpaceae (Protophyllocladoxylon , one sample), and the 'Group B' and 'Group C' Mesozoic conifers (four samples each) defined in Bamford & Philippe (2008). Of these, only Taxodioxylon had been identified previously from the Tupuangi Formation. Two new species are erected, Cupressoxylon dianneae sp. nov. and Protophyllocladoxylon jacobusii sp. nov. These records are important for understanding the mid-Cretaceous flora of New Zealand and the history of the unique modern flora of New Zealand. [ABSTRACT FROM AUTHOR]

Introduction: Podocarpus latifolius (Thunb.) R.Br. ex Mirb. is a large tree that is native to southern Africa. This species has been used extensively for multiple therapeutic purposes across the southern part of the African continent. Despite this, P. latifolius leaf extracts have not been rigorously examined growth inhibitory properties against many bacteria, including the bacterial triggers of autoimmune inflammatory diseases. Materials and Methods: The antimicrobial activity of P. latifolia leaf solvent extractions was investigated by disc diffusion and growth time course assays against some bacterial triggers of rheumatoid arthritis and ankylosing spondylitis. The growth inhibitory activity was further quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: Methanolic and aqueous P. latifolia leaf extracts were good inhibitors of P. mirabilis and K. pneumoniae. The inhibitory activity of both extracts was particularly noteworthy against the bacterial trigger of ankylosing spondylitis (K. pneumonia), with MICs of 343 and 557 µg/mL determined for the methanolic and aqueous extracts respectively. The extracts also displayed noteworthy activity against a bacterial trigger of rheumatoid arthritis (P. mirabilis), with MICs of 580 and 634 µg/mL respectively. The antibacterial activity of the methanolic and aqueous P. latifolia leaf extracts were further investigated by growth time course assays, which showed significant growth inhibition in cultures of P. mirabilis and K. pneumoniae within 1 hr of exposure. The extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for prophylactic use in preventing these autoimmune inflammatory diseases. Conclusion: The lack of toxicity of the P. latifolia leaf extracts and their growth inhibitory bioactivity against the bacterial triggers of rheumatoid arthritis and ankylosing spondylitis indicate their potential in the development of new therapies targeting the onset of these diseases. [ABSTRACT FROM AUTHOR]

Premise of research. The southern conifer family Podocarpaceae inhabits wet environments and is highly diverse in its vegetative and megasporangiate structures. The Podocarpaceae are today divided into a tropical clade and a prumnopityoid clade. We study the morphology and anatomy of leaves and megasporangiate cones at postpollination and the maturity of two native Patagonian Podocarpaceae, Saxegothaea conspicua (tropical clade) and Lepidothamnus fonkii (prumnopityoid clade). We aim to add to the knowledge of the South American Podocarpaceae and to interpret their unique morphologies to aid in our understanding of the deep and cryptic fossil record of the family and its relationships with other conifer families. Methodology. Specimens of S. conspicua and L. fonkii were collected in natural and cultivated populations of southern Chile and imaged using different macro- and microscopic techniques, including epifluorescence, bright-field, and scanning electron microscopy. Pivotal results. Leaf and megasporangiate cone anatomy and morphology are described in detail for both species. For S. conspicua , we observed a helical to slightly planate leaf arrangement with leaf reorientation, expanded leaves without accessory transfusion tissue, an apparent lack of vascularization of the epimatium (which is bikeeled and does not surround the ovule completely), and nonsaccate pollen germinating on the bract toward the protruding nucellus. For L. fonkii , we observed the absence of leaf hypodermis and resin canals, the presence of a micropylar hook in the ovule and seed integument, bisaccate pollen germinating within the ovule, cone bracts reduced in number and size, and a basal receptacle-like structure. Conclusions. Leaf arrangement in Saxegothaea shows an intermediate condition between the primary helical phyllotaxis common in the family and the secondary planate arrangement seen in most podocarps with expanded leaves. Lepidothamnus fonkii was determined to have a false receptacle (i.e., a receptacle-like structure originated from vegetative basal bracts rather than by sterile ovuliferous complexes). The presence of a false receptacle in L. fonkii is a morphological novelty in Podocarpaceae. [ABSTRACT FROM AUTHOR]

Introduction: Closely related plant species often share similar secondary metabolites and bioactivities and are therefore good targets for bioactivity testing when one or more species within a genus are known to possess therapeutic properties. The genus Podocarpus has a long history of medicinal usage in many areas of the world. Many species are known to have therapeutic properties, several species of which have well established antibacterial bioactivities. Materials and Methods: The ability of P. elatus leaf extracts to inhibit the growth of a panel of bacterial pathogens was investigated by disc diffusion assay. Toxicity was examined using the Artemia franciscana nauplii bioassay. Results: The P. elatus methanolic and aqueous extracts were ineffective at inhibiting the growth of gram-positive and gram-negative panels of bacteria and were nontoxic in the Artemia lethality assay following 24 hr exposure. Conclusion: Despite its reported therapeutic uses against other diseases and its close taxonomic relationship with several bioactive Podocarpus spp. with antibacterial activity, P. elatus leaf extracts were ineffective bacterial growth inhibitors. However, these extracts may have other therapeutic properties and testing against protozoa, fungi, virus and tumour cells is required. [ABSTRACT FROM AUTHOR]

Premise: Acmopyle (Podocarpaceae) comprises two extant species from Oceania that are physiologically restricted to ever‐wet rainforests, a confirmed fossil record based on leaf adpressions and cuticles in Australia since the Paleocene, and a few uncertain reports from New Zealand, Antarctica, and South America. We investigated fossil specimens with Acmopyle affinities from the early Eocene Laguna del Hunco site in Patagonia, Argentina. Methods: We studied 42 adpression leafy‐shoot fossils and included them in a total evidence phylogenetic analysis. Results: Acmopyle grayae sp. nov. is based on heterophyllous leafy shoots with three distinct leaf types. Among these, bilaterally flattened leaves uniquely preserve subparallel, linear features that we interpret as accessory transfusion tissue (ATT, an extra‐venous water‐conducting tissue). Some apical morphologies of A. grayae shoots are compatible with the early stages of ovuliferous cone development. Our phylogenetic analysis recovers the new species in a polytomy with the two extant Acmopyle species. We report several types of insect‐herbivory damage. We also transfer Acmopyle engelhardti from the middle Eocene Río Pichileufú flora to Dacrycarpus engelhardti comb. nov. Conclusions: We confirm the biogeographically significant presence of the endangered West Pacific genus Acmopyle in Eocene Patagonia. Acmopyle is one of the most drought‐intolerant genera in Podocarpaceae, possibly due to the high collapse risk of the ATT, and thus the new fossil species provides physiological evidence for the presence of an ever‐wet rainforest environment at Laguna del Hunco during the Early Eocene Climatic Optimum. [ABSTRACT FROM AUTHOR]

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Podocarpus is the most dominant genus of Podocarpaceae, with higher taxonomical proximity to the Taxaceae, having numerous pharmaceutical applications, however, scarce studies dealing with the physiological and metabolic criteria of Podocarpus in Egypt were reported. Thus, the objective of this work was to assess the physiological and metabolical patterns of the different species of Podocarpus; P. gracilior, P. elongates, P. macrophyllus and P. neriifolius. The highest terpenoids contents were reported in P. neriifolius, followed by P. elongatus, and P. macrophyllus. P. gracilior had the highest antioxidants amount, followed by P. macrophyllus, P. neriifolius and P. elongatus. From the GC/MS metabolic profiling, caryophyllene, β-cadinene, β-cuvebene, vitispirane, β-cadinene and amorphene were the most dominant metabolites in P. gracilior. β-Caryophyllene was the common in P. gracilior, P. elongatus, P. macrophyllus and P. neriifolius with an obvious fluctuation. The plant methanolic extracts have an obvious activity against the multidrug resistant bacteria; E. coli, P. aeruginosa, S. pyogenes and S. aureus, and fungi; A. fumigatus, A. flavus, A. niger and C. albicans in a concentration-dependent manner. The highest Taxol yield was assessed in the extracts of P. elongatus (16.4 μg/gdw), followed by P. macrophyllus, and P. neriifolius. The chemical identity of Taxol derived from P. elongatus was resolved by LC/MS, with molecular mass 854.6 m/z, and similar structural fragmentation pattern of the authentic one. The highest antitumor activity of P. elongatus extracted Taxol was assessed towards HCT-116 (30.2 μg/ml), HepG-2 (53.7 μg/ml) and MCF-7 (71.8 μg/ml). The ITS sequence of P. elongatus “as potent Taxol producer” was deposited on Genbank with accession #ON540734.1, that is the first record of Podocarpus species on Genbank.

Se presenta de forma resumida la situación anterior a esta contribución del conocimiento del subgénero Neophyllaphis (Aphididae, Neophyllaphidinae) en América del Sur, donde es alóctono. A partir de especímenes colectados en los últimos años se cita Neophyllaphis podocarpi por primera vez en Brasil y en Colombia, y Neophyllaphis varicolor por primera vez en América del Sur, en Argentina, provincia de Misiones. Se presentan los caracteres que diferencian a N. podocarpi de especies vecinas, y sucintamente su distribución conocida. Se discute la variabilidad intraespecífica morfológica y bionómica de N. varicolor, y se presenta su distribución conocida. Se reestructura la clave para la identificación de los pulgones que viven sobre plantas del género Podocarpus proporcionada en Aphids on World Plants.

The relevance of improving the knowledge on Triassic conifers resides in that the modern clades originated during the Late Triassic-Early Jurassic. More than 30 conifer-like fossil woods are known from the Triassic strata of Argentina. Whereas some have not been linked to any family, others have been related to the "Protopinaceae", Taxaceae, Podocarpaceae, Araucariaceae, Cupressaceae, or Hirmeriellaceae (=Cheirolepidiaceae). The diversity of woods with anatomy close to derived conifers contrasts with the scarce Triassic record of leaves and micro and megasporangiate cones, which could be explained by a combination of taphonomic and evolutionary causes. In order to provide information to resolve this controversy, the anatomy and systematic affinities of Triassic permineralized conifer-like trunks and woods described to date for Argentina were reassessed. As a result of this review, it emerged that the woods previously assigned to Podocarpaceae and Taxaceae do not have enough preserved characters to support such assignment, or they have characters that are homoplasic in the context of Mesozoic gymnosperms and therefore their affinity is ambiguous. On the contrary, the reevaluation of the woods related to the Araucariaceae, Cupressaceae and Hirmeriellaceae (=Cheirolepidiaceae) allows us to support their affinity with these families. [ABSTRACT FROM AUTHOR]

Although conifers have very homogeneous wood anatomical structure, some of their features allow differentiation of genera or groups of genera. Softwood anatomy is reviewed and the authors have added contributions, grouping features in five blocks (axial tracheids, axial parenchyma, rays, resin canals and mineral inclusions), with special attention to their diagnostic value at the family, genus or species levels. The discussion addresses, in axial tracheids: growth rings, intercellular spaces, pit arrangement, presence of scalloped tori, torus extensions, notches, warty layer, organic deposits, helical and callitroid thickenings, bars of Sanio and trabeculae; in axial parenchyma: presence or absence, arrangement and type of transverse end walls; in rays: composition, type of horizontal and end walls and cross-field pitting; in resin canals: epithelium and the presence of normal or traumatic resin canals; and in mineral inclusions: presence or absence. Wood structure is characterised at the family level, discussing the main elements that can be used for diagnosis at the genus level or in groups of genera. [ABSTRACT FROM AUTHOR]

The emblematic conifer flora of Chile has experienced wide-scale habitat loss and fragmentation. Coupled with the complex topography of the region, this leads to a prediction of high levels of genetic drift and isolation resulting in strong population differentiation, and the potential for negative genetic consequences. To address the degree to which these predictions are realised, we conducted a comparative population genetic survey in four endemic conifers from South America, each of conservation concern and with a restricted distribution area. Between seven and ten populations per species were sampled, covering their entire natural distribution in Chile. We used restriction site-associated DNA markers (RAD-seq), with de novo assembly and optimisation, to accommodate the large and complex genome of conifers. The main finding was low levels of genetic structure in all four conifer species (FST = 0.017–0.062). This pattern was strikingly consistent across all four species. These results are likely due to the extreme longevity of individuals restricting divergence due to genetic drift. Thus, despite the currently highly fragmented ranges of these conifer species, we did not detect evidence for high levels of population differentiation and genetic isolation. This suggests some resilience to negative genetic impacts of habitat fragmentation, and the longevity of the individual trees may provide considerable buffering capacity. We recommend follow-up studies focusing on genotyping the seedling generation, to assess whether there is any evidence for the early warning of genetic isolation and/or elevated inbreeding in the currently established cohorts. [ABSTRACT FROM AUTHOR]

Context: Biodiversity hotspots harbor 77% of endemic plant species. Patagonian Temperate Forest (PTF) is a part of a biodiversity hotspot, but over the past centuries, has been over-exploited, fragmented and replaced with exotic species plantations, lately also threatened by climate change. Objectives: Our aim is to better understand patterns of habitat suitability and niche overlap of nine endemic gymnosperm species, key elements of the PTF, complementing traditional approaches of biodiversity conservation. Methods: Using R packages and 3016 occurrence data, we deployed ecological niche models (ENM) in MaxEnt via kuenm, and classified species according to Rabinowitz's types of rarity. We then overlapped their niches calculating Schoener's D index, and considered types of rarity in a spatial ecological context. Finally, we overlay high species' suitability and protected areas and detected conservation priorities using GapAnalysis. Results: We generated simplified ENMs for nine Patagonian gymnosperms and found that most niches overlap, and only one species displayed a unique niche. Surprisingly, we found that three species have divergent suitability of habitats across the landscape and not related with previously published geographic structure of neutral genetic variation. We showed that the rarer a species is the smaller niche volume tend to have, that six out of nine studied species have high conservation priority, and that there are conservation gaps in the PTF. Conclusion: Our approach showed that there are unprotected suitable areas for native key species at high risk in PTF. Suggesting that integrating habitat-suitability models of multiple species, types of rarity, and niche overlap, can be a handy tool to identify potential conservation areas in global biodiversity hotspots. [ABSTRACT FROM AUTHOR]

El pino de monte (Podocarpus parlatorei Pilg.) es endémico de los bosques montanos australes. A lo largo de la historia fue sobreexplotado con fines maderables y actualmente se encuentra en peligro de extinción. En Bolivia existen vacíos de información con respecto a su estructura y dinámica poblacional. En este estudio describimos su estructura forestal (individuos adultos, ‘10 cm de diámetro) y su regeneración (individuos jóvenes,

The mountain pine (Podocarpus parlatorei Pilg.) is endemic to the southern montane forests, historically over-exploited for timber purposes and currently is in danger of extinction. In Bolivia, also presents information gaps regarding its population structure and dynamics. We describe its forest structure (adult individuals, ≥10 cm in diameter) and regeneration (young individuals, <10 cm) in the complex of the bolivian-tucuman pine forest in the Department of Santa Cruz (Bolivia). Sixteen localities were evaluated in the municipalities of Vallegrande, Postrer Valle, Pucara and Quirusillas (81 sampling plots). The densities (individuals/ha) of structure and regeneration were contrasted (Kruskal-Wallis tests) between municipalities and diameter categories; the association (Spearman correlations) of both variables was analyzed with each other, with elevation, slope and tree cover. The forest structure was classified into five diameter categories and was distributed in the form of an inverted J; Vallegrande presented the highest density (650 individuals/ha), higher than that determined for the landscape in general (200 individuals/ha). Regeneration, among its four diameter categories, also presented an inverted J distribution; the greatest regeneration was quantified in Pucara (1700 individuals/ha), which exceeded what was recorded at the landscape level (1100 individuals/ha). Only three variables presented significant associations (P<0.05); elevation (ϱ=0.23) and tree cover (ϱ=0.43) were positively related to forest structure, while regeneration was negatively related (ϱ=-0.30). The forest structure and the natural regeneration of the mountain pine populations revealed that the species is far from presenting a good conservation status. [ABSTRACT FROM AUTHOR]

Despite much interest and studies toward the genus Podocarpus, the anti-malarial evaluation of Podocarpus polystachyus's phytoconstituents remains lacking. Herein, the phytoconstituents of P. polystachyus leaves and their anti-malarial effect against Plasmodium falciparum were investigated for the first time. One new natural product, 8ß,13ß-kaur-15-en-17-al (1), along with three known compounds, 8ß,13ß-kaur-15-en-17-ol (2) and 13ß-kaur-16-ene (3), and α-tocopherol hydroquinone (4) were isolated via HR-ESI-MS and NMR analyses. Compounds 1 and 2 inhibited P. falciparum growth at 12 and 52 µM of IC50, respectively. Their anti-malarial activity was associated with the in silico P. falciparum lactate dehydrogenase (PfLDH) inhibition. Molecular docking of ligands 1 and 2 with the putative target PfLDH revealed ~−2 kcal/mol of binding energies more negative than the control. Molecular dynamic simulations (100 ns) showed equal or smaller deviation values (RMSD, RMSF, Rg) and stronger interactions of PfLDH-1 and PfLDH-2 complexes via at least one consistent H-bond than the control. Additionally, a slightly increased PfLDH H-bond profile in their interactions improved the PfLDH dynamic and structural stabilities. Overall, this study supports the relevance of 1 and 2 as plasmodial growth inhibitors with their putative anti-PfLDH activity, which could be a potential scaffold for developing anti-malarial drugs. [ABSTRACT FROM AUTHOR]

Parasitaxus usta (Podocarpaceae) is the only parasitic gymnosperm and endemic to New Caledonia. In this survey, 11 flavonoids and 6 phenolic compounds were isolated from the aerial parts. As for flavonoids, six flavones, apigenin 7-O-glucoside (1), luteolin (2), luteolin 7-O-glucoside (3), chrysoeriol (4), chrysoeriol 7-O-glucoside (5) and tricetin 3'-O-glucoside (6), one C-glycosylflavone, orientin (7), one flavonol, quercetin 3-O-glucoside (8), one anthocyanin, cyanidin 3-O-glucoside (9), and two biflavones, isoginkgetin (10) and agathisflavone (11) were identified by UV, liquid chromatograph-mass spectra (LC-MS), acid hydrolysis, NMR and/or HPLC comparisons with authentic samples. On the other hand, six phenolic compounds were identified as 5-O-E-p-coumaroyl quinic acid (12), 5-O-Z-p-coumaroyl quinic acid (13), 5-O-E-p-coumaroyl quinic acid methyl ester (14), 5-O-Z-p-coumaroyl quinic acid methyl ester (15), E-caffeic acid methyl ester 3-O-ß-glucopyranoside (16), and Z-caffeic acid methyl ester 3-O-ß-glucopyranoside (17) by UV, LC-MS and NMR. Chemical components of P usta were reported in this survey for the first time. Their chemical characters were chemotaxonomically compared with those of other Podocarpaceae species. [ABSTRACT FROM AUTHOR]

Nageia fleuryi (Hickel) de Laub. 1987 belongs to the genus Nageia in the family Podocarpaceae and is distributed throughout southeast Asia, including China, Vietnam, and Cambodia. It is a plant with high economic beneficial for food and construction industries. Here, we report on the complete chloroplast (cp) genome of N. fleuryi for the first time. The complete cp genome is similar to many gymnosperm plants, however, it lacks inverted repeat regions and does not possess a typical quadripartite structure. The complete cp genome is 133,870 bp in size and the overall guanine-cytosine (GC) content was found to be 37.27%. The total number of genes is 119, including 82 protein-coding genes, 33 tRNA genes, and 4 rRNA genes. Of these, 14 genes contain one intron, two genes contain two introns, and rps12 possessed a trans-splicing mechanism. Finally, the phylogenic tree demonstrated that N. fleuryi is closely related to Nageia nagi (AB830885.1 and LC572156.1)

The complete chloroplast genome sequence of Podocarpus macrophyllus was determined in this study. According to the results, the genome is 134,239 bp in length. The GC content of the whole chloroplast genome is 37.0%. The complete chloroplast genome of P. macrophyllus encodes a total of 120 genes, including 34 tRNA genes, 4 rRNA genes and 82 protein-coding genes. Like other conifers chloroplast genomes, P. macrophyllus has no inverted repeat sequences. To reveal the phylogenetic relationship of P. macrophyllus, we constructed phylogenetic trees using other species of Podocarpaceae, and the phylogenetic analysis showed that P. macrophyllus is evolutionarily closest to Podocarpus longifoliolatus.

We discuss a recent assessment by Dörken et al. (2021) regarding the affinities of the Eocene fossil species Huncocladus laubenfelsii from Laguna del Hunco (Patagonia, Argentina). We originally (Andruchow-Colombo et al. , 2019) assigned this species to the conifer family Podocarpaceae as the first certain South American macrofossil record of the phyllocladoid lineage (Huncocladus + Phyllocladus), based on a combination of numerous macro- and micromorphological vegetative characters. However, Dörken et al. (2021) rejected the podocarpaceous affinity of H. laubenfelsii and considered it to be more closely related to the cycad genera Bowenia or Eobowenia. Their assessment was based almost entirely on two cuticular characters, with only superficial consideration of the abundant additional evidence available that included several diagnostic macromorphological features. We review the two characters mentioned by these authors, and other features, and find that their suggestion is contradicted by the available evidence, maintaining our original assignment. Critical characters include presence/absence of a midvein, secondary venation pattern, arrangement and general morphology of the photosynthetic structures, and morphology and disposition of epidermal cells. [ABSTRACT FROM AUTHOR]

Prumnopitys andina is a South American podocarp with a restricted distribution in southern Andean temperate forests, and is severely threatened due to habitat loss. Despite much dendrochronological work on other temperate South American conifers, little is known about the dendrochronological potential of P. andina. Here, we examine the main features of P. andina tree rings and evaluate the potential of this species for yielding tree-ring chronologies sensitive to climatic variability. In a population at the southern range limit of the species, we collected tree cores and cross-section samples of P. andina for analyses following standard dendrochronological techniques. 76 tree-ring series from 45 trees were examined, 32 (42%) of which were successfully cross-dated and included in a chronology. We found that the species forms well-defined tree rings with distinct boundaries delimiting annual growth. The chronology began in 1778, with notable periods of suppression and release occurring in the late nineteenth and early twentieth centuries, respectively. The chronology showed a high inter-series correlation value of 0.51, suggesting a strong common signal among trees. December to January precipitation showed a positive relationship with radial growth. Conversely, maximum and mean temperature showed negative relationships with radial growth during the austral summer and autumn. These significant relationships of climate variables with radial growth of P. andina indicate that tree-ring series of this species are suitable for dendroclimatological studies. [ABSTRACT FROM AUTHOR]

Background and Aims The three relict genera Pherosphaera , Microcachrys and Saxegothaea in Podocarpaceae produce quite distinct seed cone types in comparison with other genera and do not form a clade along with Acmopyle. The detailed seed cone morpho-anatomy of these three relict genera and affinities with other podocarps are poorly known. This study aims to understand the seed cone morpho-anatomy and affinities among these three disjunct relict genera and with other podocarps. Methods We comparatively analysed the seed cone morpho-anatomical traits of the three podocarps genera and used ancestral state reconstruction to understand the evolution of these traits. Key Results We described the seed cone morpho-anatomical structures of the three relict genera in detail. The three genera produce aggregated multiovulate cones. Both Microcachrys and Saxegothaea have an asymmetrical free cup-like epimatium. Both species of Pherosphaera lack an epimatium. The ancestral state reconstruction implies that the presence of an epimatium is an ancestral trait in podocarps and is independently lost in Pherosphaera and Phyllocladus. The seed cones are fleshy in Microcachrys and non-fleshy in Saxegothaea and Pherosphaera. The seed cone macrofossils of both extinct and living podocarps also show the presence of an epimatium and fleshiness in podocarps. Conclusions Altogether, the morpho-anatomy suggests that Pherosphaera , Microcachrys and Saxegothaea present affinities with each other and other podocarps, but the reconstruction of the ancestral seed cone in Podcarpaceae is quite complex due to multiple convergent evolutions of several structures. These structures (e.g. epimatium, aril and receptaculum) are of low taxonomic value but of great evolutionary and ecological significance, and are responsive adaptations to ever-changing environmental conditions. [ABSTRACT FROM AUTHOR]

Eocene paleofloras of Patagonia are diverse and increasingly known. A new assemblage of fossil wood has been recovered from Eocene sediments in Corcovado, western Argentinean Patagonia. The lithological succession (formerly Arroyo Lyn Formation sensu Pesce 1979) is correlated with the Huitrera Formation. The specimens were mostly found embedded in sandstones and conglomerates. One-third of the assemblage are conifers and studied herein. We found four taxonomic types: Agathoxylon cf. antarcticum (Araucariaceae), Phyllocladoxylon antarcticum (Podocarpaceae), Podocarpoxylon dusenii (Podocarpaceae), and Cupressinoxylon hallei (Cupressaceae or Podocarpaceae). The presence of four taxonomic units among only 7 specimens suggests a significant conifer species richness in the assemblage. Araucariaceae and dominant Podocarpaceae are usually found in previously described conifer wood assemblages from the Eocene of Patagonia andAntarctica. The diversity of the conifer assemblage in Corcovado is very similar to that found at Laguna del Hunco (these two localities are 170 km distant), also from the Huitrera Formation. This is consistent with the proposal that the bearing sediments of both localities are from the same stratigraphic unit. [ABSTRACT FROM AUTHOR]

Premise: The sooty molds are a globally distributed ecological group of ascomycetes with epiphyllous, saprotrophic habit, comprising several phylogenetically distant taxa (i.e., members of the classes Dothideomycetes and Eurotiomycetes). Their fossil record extends almost continuously back to the early Cretaceous; however, they are hypothesized to have originated in the early Mesozoic. Here, we describe new specimens of sooty molds associated with conifer leaves from Jurassic hot spring deposits of Patagonia, Argentina. Methods: Thin sections of chert samples from the La Matilde Formation, Deseado Massif (Santa Cruz, Argentina) were observed using light microscopy. Results: The fungi occur on the surface and axils of leafy twigs with podocarpaceous affinities, forming dense subicula comprised by opaque moniliform hyphae. Additionally, several asexual and sexual reproductive structures are observed. On the basis of vegetative (i.e., dense subicula composed of moniliform hyphae; hyphae composed of opaque cells deeply constricted at the septa) and reproductive characters (i.e., poroconidial and sympodioconidial asexual stages and diverse spores), two morphotypes were identified with affinities within lineages of the subphylum Pezizomycotina that encompass the ecological group of sooty molds, and a third morphotype was within the phylum Ascomycota. Conclusions: This finding extends the fossil record of sooty molds to the Jurassic and their geographic fossil range to the South American continent. In particular, their association with podocarpaceous conifers is shown to be ancient, dating back to the Jurassic. This new record provides an additional reference point on the diversity of interactions that characterized Jurassic forests in Patagonia. [ABSTRACT FROM AUTHOR]

Despite much interest and studies toward the genus Podocarpus, the anti-malarial evaluation of Podocarpus polystachyus’s phytoconstituents remains lacking. Herein, the phytoconstituents of P. polystachyus leaves and their anti-malarial effect against Plasmodium falciparum were investigated for the first time. One new natural product, 8ß,13ß-kaur-15-en-17-al (1), along with three known compounds, 8ß,13ß-kaur-15-en-17-ol (2) and 13ß-kaur-16-ene (3), and α-tocopherol hydroquinone (4) were isolated via HR-ESI-MS and NMR analyses. Compounds 1 and 2 inhibited P. falciparum growth at 12 and 52 µM of IC50, respectively. Their anti-malarial activity was associated with the in silico P. falciparum lactate dehydrogenase (PfLDH) inhibition. Molecular docking of ligands 1 and 2 with the putative target PfLDH revealed ~−2 kcal/mol of binding energies more negative than the control. Molecular dynamic simulations (100 ns) showed equal or smaller deviation values (RMSD, RMSF, Rg) and stronger interactions of PfLDH-1 and PfLDH-2 complexes via at least one consistent H-bond than the control. Additionally, a slightly increased PfLDH H-bond profile in their interactions improved the PfLDH dynamic and structural stabilities. Overall, this study supports the relevance of 1 and 2 as plasmodial growth inhibitors with their putative anti-PfLDH activity, which could be a potential scaffold for developing anti-malarial drugs.

The modern genus Dacrycarpus (Endl.) de Laub. of the family Podocarpaceae, containing nine species, is mainly distributed in tropical mountain rainforests of the southwestern Pacific region, ranging from New Zealand to low‐latitude Asia. This genus has abundant fossil records in both hemispheres, but all the known megafossils were limited to Australasia and South America. Here we report on Dacrycarpus guipingensis sp. nov. from the Miocene Erzitang Formation of Guangxi, South China. This is the first megafossil of Dacrycarpus in the Northern Hemisphere. The new species is represented by mummified dimorphic foliage, ovuliferous shoots, and a male cone with in situ pollen. It resembles the extant Dacrycarpus imbricatus (Blume) de Laub., which is common in rainforests from southern China and northern Myanmar to Fiji. This paper presents the first data on the anatomical structure of seed cone and exine ultrastructure of Dacrycarpus in situ pollen grains from a fossil material by using computed tomography scanning and ultrathin sectioning. For comparative purpose, data on the pollen morphology and ultrastructure were obtained for modern D. imbricatus for the first time. The D. guipingensis fossils strongly suggest the Miocene arrival of Dacrycarpus in Asia from the Southern Hemisphere. Based on the modern ecological niche and related fossil elements, this fossil locality was probably covered by conifer–broad‐leaved mountain rainforests during the Miocene. [ABSTRACT FROM AUTHOR]

The ulcumano (Retrophyllum rospigliosii) is an important native conifer of Peru, which is currently disappearing from the areas where it was dominant, due to its excessive use and problems in its regeneration. Also, there is limited information on its vegetative propagation. For this reason, the objective was to determine the efficiency of the vegetative propagation of the ulcumano in the sub-irrigation chamber. Four concentrations of IBA were used (0, 1 000, 3 000 and 5 000 ppm), two ages of mother plants (two and eight years) and two types of cuttings (apical and middle); under a completely random factorial design. The results indicate that, at the end of 17 weeks, the T7 treatment (corresponding to medium type cuttings, from two-year-old mother plants and treated with 3 000 ppm IBA) obtained the highest rooting (40%), root length (7 mm) and number of roots per cutting (2.2). Auxin AIB didn't influence rooting. Type cuttings influenced survival, rooting, and sprouting. The interaction between the cuttings mother plant and type of s had a significant influence on rooting success, two-year-old trees and medium-type cuttings obtained higher survival (76.85%), rooting (32.5%) and sprouting (70%). It is concluded that it is possible to propagate ulcumano using middle cuttings of two-year-old trees. [ABSTRACT FROM AUTHOR]

Prumnopitys andina (lleuque) es una especie de conífera con una distribución restringida en Chile y Argentina. A pesar de su importancia ecológica, los conocimientos sobre la estructura y dinámica de los bosques donde crece P. andina son limitados. El objetivo de este trabajo fue describir la estructura y regeneración de bosques con alta presencia de P. andina. Se muestrearon bosques cercanos a Lonquimay (38°1' S, 71.3 O – 38°3'S, 71°3' O) midiendo árboles, regeneración y flora vascular a través de 24 parcelas. Se encontró que P. andina ocurre predominantemente en el estrato superior (15-18 m) acompañado por Nothofagus obliqua y/o Austrocedrus chilensis, además de Lomatia hirsuta y Maytenus boaria en estratos intermedios (4-8 m). En base a la estructura, se identificaron tres tipos de bosques de P. andina: bosques abiertos, bosques secundarios adultos y bosques puros. Los bosques puros de P. andina o “lleuquerías” alcanzaron áreas basales entre las más grandes reportadas para bosques templados del sur de Sudamérica (230 m2/ha). La regeneración de P. andina osciló entre 1.550 individuos/ha en bosques abiertos y 25.000 individuos/ha en bosques puros. P. andina exhibió una estrategia de regeneración aparentemente continua, y en ausencia de disturbios antrópicos como pastoreo de ganado o tala selectiva, sus poblaciones serían autosuficientes.

During the early Eocene, Patagonia had highly diverse floras that are primarily known from compression and pollen fossils. Fossil wood studies from this epoch are scarce in the region and largely absent from the Laguna del Hunco flora, which has a highly diverse and excellently preserved compression assemblage. A collection of 26 conifer woods from the Laguna del Hunco fossil-lake beds (early Eocene, ca. 52 Ma) from central-western Patagonia was studied, of which 12 could be identified to genus. The dominant species is Phyllocladoxylon antarcticum, which has affinity with early-diverging Podocarpaceae such as Phyllocladus and Prumnnopitys. A single specimen of Protophyllocladoxylon francisiae probably represents an extinct group of Podocarpaceae. In addition, two taxonomic units of cf. Cupressinoxylon with putative affinity to Podocarpaceae were found. Diverse Podocarpaceae taxa consistent with the affinities of these woods were previously reported from vegetative and reproductive macrofossils as well as pollen grains from the same source unit. Some of the woods have galleries filled with frass. Distinct growth ring boundaries indicate seasonality, inferred to represent seasonal light availability. Growth ring widths suggest that the woods came from mature trees, whereas the widths and types of some rings denote near-uniform temperature and water availability conditions. [ABSTRACT FROM AUTHOR]

Background and Aims Afromontane forests host a unique biodiversity distributed in isolated high-elevation habitats within a matrix of rain forests or savannahs, yet they share a remarkable flora that raises questions about past connectivity between currently isolated forests. Here, we focused on the Podocarpus latifolius–P. milanjianus complex (Podocarpaceae), the most widely distributed conifers throughout sub-Saharan African highlands, to infer its demographic history from genetic data. Methods We sequenced the whole plastid genome, mitochondrial DNA regions and nuclear ribosomal DNA of 88 samples from Cameroon to Angola in western Central Africa and from Kenya to the Cape region in eastern and southern Africa to reconstruct time-calibrated phylogenies and perform demographic inferences. Key Results We show that P. latifolius and P. milanjianus form a single species, whose lineages diverged during the Pleistocene, mostly between approx, 200 000 and 300 000 years BP, after which they underwent a wide range expansion leading to their current distributions. Confronting phylogenomic and palaeoecological data, we argue that the species originated in East Africa and reached the highlands of the Atlantic side of Africa through two probable latitudinal migration corridors: a northern one towards the Cameroon volcanic line, and a southern one towards Angola. Although the species is now rare in large parts of its range, no demographic decline was detected, probably because it occurred too recently to have left a genetic signature in our DNA sequences. Conclusions Despite the ancient and highly fluctuating history of podocarps in Africa revealed by palaeobotanical records, the extended distribution of current P. latifolius/milanjianus lineages is shown to result from a more recent history, mostly during the mid-late Pleistocene, when Afromontane forests were once far more widespread and continuous. [ABSTRACT FROM AUTHOR]

Phylogeography and species distribution models (SDMs) were integrated to test the hypothesis that the current disjunct distribution of Podocarpus lambertii, a cold-tolerant species, preserves the signature of Quaternary climatic oscillations, revealing historical barriers to gene flow. Twenty-five populations (286 individuals) of P. lambertii were screened for sequence variation at three intergenic cpDNA loci. We characterized the genetic diversity, constructed a haplotype network, identified population groups based on genetic divergence among populations and putative refugial areas, and tested the association between genetic and geographic distances. For SDMs, we used an ensemble approach based on five algorithms to estimate climatically suitable areas for the species during the Last Glacial Maximum (LGM), Holocene (HOL), and the present-day (PD). For all populations, haplotype and nucleotide diversity were 0.676 and 4.8 × 10−4, respectively. Neutrality tests indicated a recent expansion for the species. Analysis of molecular variance revealed genetic divergence among populations (FST = 0.1385), significantly correlated with geographic distances (r = 0.524, p < 0.01). Bayesian analysis suggested the formation of a Southern Group (SG) and Northern Group (NG), separated by a barrier to gene flow, with high differentiation among groups (FCT = 0.6458). The SDMs showed temporal changes in an area suitable for P. lambertii, mainly present at high altitudes and cold temperatures. The main occurrence area of P. lambertii now resides in SG, owing to a colder climate when compared with NG and the expansion of Araucaria angustifolia, a nurse plant that may have created better microclimatic conditions for P. lambertii expansion. [ABSTRACT FROM AUTHOR]

Sites recording the extinction or extirpation of tropical–subtropical and cool–cold temperate rainforest genera during the Plio–Pleistocene aridification of Australia are scattered across the continent, with most preserving only partial records from either the Pliocene or Pleistocene. The highland Lake George basin is unique in accumulating sediment over c. 4 Ma although interpretation of the plant microfossil record is complicated by its size (950 km2), neotectonic activity and fluctuating water levels. A comparison of this and other sites confirms (1) the extinction of rainforest at Lake George was part of the retreat of Nothofagus -gymnosperm communities across Australia during the Plio–Pleistocene; (2) communities of warm- and cool-adapted rainforest genera growing under moderately warm-wet conditions in the Late Pliocene to Early Pleistocene have no modern analogues; (3) the final extirpation of rainforest taxa at Lake George occurred during the Middle Pleistocene; and (4) the role of local wildfires is unresolved although topography, and, elsewhere, possibly edaphic factors allowed temperate rainforest genera to persist long after these taxa became extinct or extirpated at low elevations across much of eastern Australia. Araucaria , which is now restricted to the subtropics–tropics in Australia, appears to have survived into Middle Pleistocene time at Lake George, although the reason remains unclear. Lake George near Canberra is unique in Australia. It was created by tectonic uplift of the Lake George Range, and now preserves a 3–4-million-year microfossil history of plants growing on the Southern Tablelands. These microfossils demonstrate that rainforest species now found only in Tasmania, New Zealand, Papua New Guinea and New Caledonia grew around the shoreline and on the Range 2–3 million years ago before being replaced by dry forests and grasslands as climates across Australia became increasingly cool and arid. [ABSTRACT FROM AUTHOR]

The complete chloroplast (cp) genome sequence of Podocarpus neriifolius D. Don was determined by BGISEQ-500 platform sequencing in this study. The entire cp genome was determined to be 133,878 bp in length. The genome contains a total of 119 encoding genes, including 82 protein-coding genes, four rRNA genes, and 33 tRNA genes. The overall GC content of P. neriifolius genome is 37.1%. A phylogenetic tree reconstructed by other 18 conifer species illustrated that P. neriifolius was the nearest relative to P. totara, P. latifolius, and P. lambertii.

The five mediterranean-type climate (MTC) regions of the world, notable for their globally significant levels vascular plant diversity and endemism, fall into two groups. The Cape Region of South Africa and southwestern Australia are ancient and relatively climatically and tectonically stable landscapes with very high species richness relative to their area. This richness reflects a core of paleoendemic lineages as well as Neogene diversification. In contrast, California, the Mediterranean Basin, and central Chile are young climatically and tectonically dynamic landscapes that have developed significant floras in the Neogene. This relative pattern of species richness breaks down when only conifers are considered. Collectively the five MTC regions have a conifer flora of 122 species, almost 20% of the global total, and are rich in endemic conifers species. This diversity is heavily centered in California with 58 species (31 endemic) and the Mediterranean Basin with 40 species (32 endemic). The key to success of conifers in the Northern Hemisphere MTC regions has been the evolution of functional hydraulic traits associated with cold and/or drought tolerance, as exemplified by Neogene diversification in many clades of Pinaceae and Cupressaceae, and by ecophysiological traits that offer competitive advantages over angiosperms on oligotrophic soils. Podocarpaceae and many other Southern Hemisphere conifer lineages never developed equivalent traits and were poorly adapted to surviving increasingly cool and strongly seasonal climate regimes with associated aridity in the late Neogene. Because of extinctions, conifer lineages in the Southern Hemisphere today exhibit a significantly older distribution of divergence ages than their counterparts in the Northern Hemisphere and are home to many isolated relict taxa. This can be seen well in Chile and the Cape Region where there has been little or no Neogene diversification of conifers and a preponderance of relict Gondwanan lineages remain. Only Callitris in southwestern Australia among the Southern Hemisphere MTC floras has been able to evolve successful ecophysiological traits to adapt to aridity in a fire-prone environment and diversify. Traits of cold, drought, and low nutrient tolerance of Northern Hemisphere conifers, most notably in the Pinaceae, have led to their widespread introduction in Southern Hemisphere agroforestry.

• Podocarpus matudae , P. guatemalensis and P. oleifolius are common trees in northern Mesoamerica. • Evolutionary history was inferred using 255 Podocarpus cpDNA sequences and past distributions. • Genetic and environmental differentiation revealed highland and lowland lineages with intermingled individuals. • Long-term persistence and elevational spread of suitable habitat was predicted during the LGM. The biogeographical history of Mesoamerican cloud forests is complex, encompassing a diverse and heterogeneous mixture of species with temperate and tropical origins. The dynamic geological landscape and climate change from the Miocene to the Pleistocene affected the distributions and composition of cloud forests in the region, and contributed to divergence events at different time scales. We assessed genetic variation of 29 populations of P. matudae , and closely related P. guatemalensis and P. oleifolius (Podocarpaceae) by sequencing 255 samples of the psb A- trn H and trn L-F intergenic spacer regions across the species ranges. We conducted phylogenetic, population and spatial genetic analyses as well as divergence time estimation and ecological niche modelling (ENM) to test the generality of demographic and genetic scenarios for cloud forest-adapted species. The results revealed genetic differentiation among species, with some individuals of P. oleifolius and P. guatemalensis placed in the P. matudae group and some P. oleifolius in the P. guatemalensis group. Predictions of ENMs under past climatic conditions and a strong signal of spatial expansion suggest that the highland P. matudae and P. oleifolius populations experienced expansions into lower elevation during the Last Glacial Maximum (LGM). Contrary to predictions by the two precipitation models and elevational ups and downs for cloud forest taxa during the LGM, genetic differentiation and predicted distribution of suitable habitat support the hypotheses that P. matudae and P. oleifolius remained in situ during the LGM primarily within the current fragmented distribution of the cloud forest and spread into the lowlands during the LGM, whereas the distribution of suitable habitat for P. guatemalensis had no major changes upwards from the Last Inter Glacial (LIG) to current conditions. [ABSTRACT FROM AUTHOR]

Podocarpus latifolius [(Thunb.) R.Br. ex Mirb.], also known as real yellow wood, is a large evergreen tree with exceptionally high-quality wood. It is a member of the Podocarpaceae family, which includes many species widely grown for wood pulp as well as timber for construction. Despite its importance, studies focusing on its genetic characterization and molecular biology are limited. Therefore, this study reports the complete plastome of P. latifolius, which is a circular molecule of 134 020 base pairs (bp) in length, lacking a quadripartite structure. The P. latifolius plastome encodes 117 unique genes, consisting of 82 protein-coding genes, 31 transfer RNA genes and four ribosomal RNA genes. The analysis showed that the Podocarpaceae plastomes have experienced some intron and gene losses, inversions, and inverted repeat (IR) loss resulting in a diverse plastome organization at the species and genus levels. Therefore, to understand the extent of these genomic rearrangements, more sampling of the Podocarpaceae plastomes is necessary. A total of 149 editing sites were predicted in 28 genes, all of which were C to U conversions. Moreover, a total of 164 simple sequence repeats (SSRs) were identified in the P. latifolius plastome, the majority being mononucleotide repeat motifs with A/T sequence predominance. Overall, the data obtained in this study will be useful for population genetics, evolutionary history and phylogenetic studies of the species in this genus. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • Lagarostrobos franklinii has the largest plastome of cupressophytes known to date. • Lagarostrobos plastome is enriched with repeats and intergenic spacers. • Plastid phylogenomics divides the Podocarpaceae into two large clades. • Podocarpoid-Dacrydioid- Pherosphaera - Saxegothaea clade is sister to Prumnopityoid. • Phyllocladus is nested within the Prumnopityoid clade. Abstract Podocarpaceae is the largest family in cupressophytes (conifers II), but its plastid genomes (plastomes) are poorly studied, with plastome data currently existing for only four of the 19 Podocarpaceous genera. In this study, we sequenced and assembled the complete plastomes from representatives of eight additional genera, including Afrocarpus, Dacrydium, Lagarostrobos, Lepidothamnus, Pherosphaera, Phyllocladus, Prumnopitys, and Saxegothaea. We found that Lagarostrobos, a monotypic genus native to Tasmania, has the largest plastome (151,496 bp) among any cupressophytes studied to date. Plastome enlargement in Lagarostrobos coincides with increased intergenic spacers, repeats, and duplicated genes. Among the Podocarpaceae, Lagarostrobos has the most rearranged plastome, but its substitution rates are modest. Plastid phylogenomic analyses based on 81 plastid genes clarify the positions of previously conflicting Podocarpaceous genera. Tree topologies firmly support the division of Podocarpaceae into two sister clades: (1) the Prumnopityoid clade and (2) the clade containing Podocarpoid, Dacrydioid, Pherosphaera, and Saxegothaea. The Phyllocladus is nested within the Podocarpaceae, thus familial status of the monotypic Phyllocladaceae is not supported. [ABSTRACT FROM AUTHOR]

The first host record for Vervoortihelcon scaramozzinoi van Achterberg, 1998 is presented and additional notes on its morphology are provided and illustrated. The species is recorded as parasitizing the cerambycid beetle Stenorhopalus rubiginus in Podocarpus L'Hér ex Pers (Podocarpaceae). The metasomal carapace is shown to be sexually dimorphic and comprising 4 tergites in females but 5 in males. Some aspects of metasomal sculpture are reported as being variable and others highly consistent between individuals and differing somewhat from the original description. [ABSTRACT FROM AUTHOR]

Endophytic fungi have been considered as a repertoire for bioactive secondary metabolites with potential application in medicine, agriculture and food industry. The biosynthetic pathways by fungal endophytes raise the argument of acquisition of these machineries of such complex metabolites from the plant host. Diterpenoids “Taxol” is the most effective anticancer drug with highest annual sale, since its discovery in 1970 from the Pacific yew tree, Taxus brevifolia. However, the lower yield of Taxol from this natural source (bark of T. brevifolia), availability and vulnerability of this plant to unpredicted fluctuation with the ecological and environmental conditions are the challenges. Endophytic fungi from Taxus spp. opened a new avenue for industrial Taxol production due to their fast growth, cost effectiveness, independence on climatic changes, feasibility of genetic manipulation. However, the anticipation of endophytic fungi for industrial Taxol production has been challenged by the loss of its productivity, due to the metabolic reprograming of cells, downregulating the expression of its encoding genes with subculturing and storage. Thus, the objectives of this review were to (1) Nominate the endophytic fungal isolates with the Taxol producing potency from Taxaceae and Podocarpaceae; (2) Emphasize the different approaches such as molecular manipulation, cultural optimization, co-cultivation for enhancing the Taxol productivities; (3) Accentuate the genome mining of the rate-limiting enzymes for rapid screening the Taxol biosynthetic machinery; (4) Triggering the silenced rate-limiting genes and transcriptional factors to activates the biosynthetic gene cluster of Taxol.

In this study, the complete chloroplast genome of Dacrydium pierrei was reported. The results showed that the whole genome was 131,671 bp in length. The genome contains a total of 118 encoding genes, including 82 protein-coding genes, 32 tRNA genes, and 4 rRNA genes. Fourteen genes contain one intron and ycf3 gene contains two introns. The GC content of the entire genome is 37.25%. The maximum likelihood phylogenetic analysis based on 59 chloroplast genes from 25 gymnosperm taxa showed that D. pierrei was sister to Podocarpus imbricatus.