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The paper analyzes the static behavior and the seismic vulnerability of the “San Francesco ad Alto” building in Ancona (Italy), which is currently used as a Regional Headquarter of the Marche Region by the Italian Army and was formerly a monastery. The global static structural behavior and the dynamic properties have been evaluated using the Finite Element modeling technique, in which the nonlinear behavior of masonry has been taken into account by proper constitutive laws. The concepts of homogenized material and smeared cracking are used to evaluate the capacity of the monastery to withstand lateral loads together with the expected demands resulting from seismic actions (N2 method), using a nonlinear static analysis (pushover). The comparison of seismic demand and capacity confirms the susceptibility of these types of buildings to extensive damage and collapse, as frequently observed in similar buildings. This paper aims to point out that advanced numerical analyses can offer significant information on the understanding of the actual structural behavior of historical buildings. It is believed that the methodology and the overall conclusions of this case study are valid for many historical monasteries in Europe. [ABSTRACT FROM AUTHOR]