Search

Byline: A. I. Nikolaev (1), V. I. Dubovaya (2), D. Yu. Litvinov (2), A. B. Poltaraus (2), D. S. Ivanov (2), A. M. Amchenkova (3), A. N. Narovlyanskii (3), A. F. Panasyuk (4), V. S. Prasolov (2), K. T. Turpaev (2) Keywords: nitric oxide; gene induction; chondrocytes; subtraction hybridization; mcl1; COX2; cyclooxygenase Abstract: Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor. Author Affiliation: (1) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia (2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia (3) Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, 230981, Russia (4) Institute of Rheumatology, Russian Academy of Medical Sciences, Moscow, 115522, Russia Article History: Registration Date: 09/10/2004

Byline: L. V. Dergunova (1), N. M. Raevskaya (1), I. P. Vladychenskaya (1), A. B. Poltaraus (2), E. A. Klimov (3), I. G. Udina (3), G. E. Sulimova (3), S. A. Limborska (1) Keywords: brain-specific cDNAs; human genome; gene expression; chromosomal location; RT-PCR; 3'-untranslated region; complexin 2 Abstract: A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 cosmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBL Y15167) isolated previously from a cDNA library of the frontal cerebral cortex, the primary structure was established for genomic clone Ghfb sized more than 4 kb. A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878--933) of the human complexin 2 mRNA. Large transcripts with the 5' end corresponding to the complexin 2 mRNA and the 3' end to Ghfb were detected in total mRNA of the human brain by means of RT-PCR. The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb. Author Affiliation: (1) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia (2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia (3) Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117809, Russia Article History: Registration Date: 08/10/2004

Two copies of the 16S rRNA gene, rrnAand rrnB, of the type strain 17Tof the thermophilic sulfate-reducing bacterium Desulfotomaculum kuznetsoviiwere cloned and completely sequenced. The comparison of the determined sequences revealed considerable heterogeneity (8.3%) of the two genes, rrnAand rrnB.The main differences were associated with superlong inserts located in the variable 5"- and 3"-terminal regions of the 16S rRNA genes. Comparative analysis that involved analogous genes from the phylogenetically closest representatives of the genus Desulfotomaculumshowed that disregard of the heterogeneity of the two gene copies distorts the position of the bacterium studied in the phylogenetic tree. [ABSTRACT FROM AUTHOR]

The controversial phylogenetic position of the recently described South-East Asian endemic bovid, Pseudonovibos spiralis, was evaluated on the basis of phylogenetic analyses of originally obtained nearly complete 12S mitochondrial rDNA sequences for this species and Bubalus bubalis and 26 sequences of Bovidae from the Genbank using Cervus elaphus (Cervidae) as outgroup. In most of the phylogenetic analyses performed using PAUP 4.0 (maximum parsimony, maximum likelihood and neighbour-joining), Bovidae consisted of two major clades: Bovinae including the tribes Bovini, Tragelaphini and Boselaphini, and Antilopinae+Caprinae, incorporating all other bovids. In most trees P. spiralis fell within the buffalos (subtribe Bovina) between Bubalus and Syncerus. Therefore, our phylogenetic analyses of bovid mitochondrial 12S rRNA gene sequences suggest the close relationship of this enigmatic species with the buffalos and its placement within the subtribe Bovina. [ABSTRACT FROM AUTHOR]