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144 results on '"Polywka, Susanne"'

1. BCMA x CD3 T-cell engager in a patient with penta-refractory multiple myeloma and HIV: a clinical and immunological report

Author

Cords, Leon, primary, Schaefers, Christoph, additional, Kamili, Abdulaziz, additional, Hoffmann, Christian, additional, Cichutek, Sophia, additional, Haag, Friedrich, additional, Polywka, Susanne, additional, Bokemeyer, Carsten, additional, Leypoldt, Lisa, additional, Alsdorf, Winfried, additional, Wiesch, Julian Schulze zur, additional, and Weisel, Katja C., additional

Published
2024
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2. Proof of infectivity of hepatitis E virus particles from the ejaculate of chronically infected patients.

Author

Schemmerer, Mathias, Bock, Hans H., Schattenberg, Jörn M., Huber, Samuel, Polywka, Susanne, Mader, Maria, Lohse, Ansgar W., Todt, Daniel, Steinmann, Eike, Wenzel, Jürgen J., Horvatits, Thomas, and Pischke, Sven

Subjects
HEPATITIS E virus, VIRAL load, VIRAL shedding, CELL culture, SEXUAL partners
Abstract

Recently, hepatitis E virus (HEV, Paslahepevirus balayani) particles were detected for the first time in the ejaculate of two chronically infected patients. Since then, we have been able to detect HEV in ejaculate in five further patients, and thus in a total of seven out of nine (78%) chronically infected men (age 36–67 years, median 56 years). In five patients, the HEV RNA concentration was more than 100‐fold higher compared to the serum, while in two patients, the viral load was more than 10‐fold lower. However, it has remained unclear whether viral particles shed in the ejaculate were infectious, as a previous cell culture model had failed to demonstrate the infectivity. In the current study, we employed an optimized HEV cell culture system based on overconfluent PLC/PRF/5 cells to investigate the infectivity of HEV particles from ejaculate and other body fluids. With this approach, we were able to show for the first time that HEV particles in the ejaculate from several patients were infectious. HEV replicated to high viral loads of 1e9 HEV RNA copies per ml. This indicates that HEV‐positive ejaculate could bear a risk of infection for sexual partners. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Relevance of HEV detection in ejaculate of chronically infected patients

Author

Schemmerer, Mathias, primary, Luetgehetmann, Marc, additional, Bock, Hans, additional, Schattenberg, Jörn, additional, Huber, Samuel, additional, Polywka, Susanne, additional, Mader, M, additional, Lohse, Ansgar, additional, Todt, Daniel, additional, Steinmann, Eike, additional, Wenzel, Jürgen, additional, Horvatits, Thomas, additional, and Pischke, Sven, additional

Published
2022
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9. Clinical features of hepatitis E infections in patients with hematologic disorders

Author

Ghandili, Susanne, primary, Lindhauer, Cecilia, additional, Pischke, Sven, additional, Zur Wiesch, Julian Schulze, additional, Von Kroge, Philipp H., additional, Polywka, Susanne, additional, Bokemeyer, Carsten, additional, Fiedler, Walter, additional, Kröger, Nicolaus, additional, Ayuk, Francis, additional, Adjallé, Raissa, additional, and Modemann, Franziska, additional

Published
2022
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12. Guidelines for the treatment of hepatitis C virus infection in injection drug users: status quo in the European Union countries

Author

Reimer, Jens, Schulte, Bernd, Castells, Xavier, Schafer, Ingo, Polywka, Susanne, Hedrich, Dagmar, Wiessing, Lucas, Haasen, Christian, Backmund, Markus, and Krausz, Michael

Subjects
Europe -- Health policy, Medical protocols -- Research, Drug addicts -- Health aspects, Drug addicts -- Care and treatment, Hepatitis C virus -- Care and treatment, Hepatitis C virus -- Complications and side effects, Health, Health care industry
Published
2005

17. Significance of Anti-Nuclear Antibodies and Cryoglobulins in Patients with Acute and Chronic HEV Infection

Author

Horvatits, Thomas, primary, Schulze zur Wiesch, Julian, additional, Polywka, Susanne, additional, Buescher, Gustav, additional, Lütgehetmann, Marc, additional, Hussey, Elaine, additional, Horvatits, Karoline, additional, Peine, Sven, additional, Haag, Friedrich, additional, Addo, Marylyn M., additional, Lohse, Ansgar W., additional, Weiler-Normann, Christina, additional, and Pischke, Sven, additional

Published
2020
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20. SAT-208-Zinc/Ribavirin: A possible treatment option in chronically HEV genotype 3 infected patients without SVR under ribavirin monotherapy

Author

Horvatits, Thomas, primary, Schübel, Niels, additional, Kamar, Nassim, additional, Polywka, Susanne, additional, Lütgehetmann, Marc, additional, Rutter, Karoline, additional, Wiesch, Julian Schulze zur, additional, Manns, Michael P., additional, Lohse, Ansgar, additional, Roque-Afonso, Anne Marie, additional, Pischke, Sven, additional, Behrendt, Patrick, additional, and Mallet, Vincent, additional

Published
2019
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23. Hepatitis delta virus propagation enabled by hepatitis C virus—Scientifically intriguing, but is it relevant to clinical practice?

Author

Pflüger, Lisa Sophie, Schulze zur Wiesch, Julian, Polywka, Susanne, and Lütgehetmann, Marc

Subjects
HEPATITIS D virus, HEPATITIS C virus, HEPATITIS B virus, HEPATITIS associated antigen, PATHOGENIC viruses, DENGUE viruses
Abstract

In vitro cell culture experiments and animal models have demonstrated that hepatitis delta virus (HDV) can theoretically propagate being enveloped by human pathogenic viruses other than hepatitis B virus (HBV), namely hepatitis C virus (HCV) and dengue virus. However, the clinical relevance of these findings and whether HDV replication occurs in real‐world hepatitis B surface antigen (HBsAg)–negative HCV patient cohorts remain unknown. To this aim, we analysed 323 HCV‐RNA–positive and HBsAg‐negative sera for the presence of HDV‐RNA and anti‐HDV antibodies (anti‐HDV). All 323 (100%) samples were negative for HDV‐RNA. Interestingly, 8/316 samples tested positive for anti‐HDV. The HBV serology of these eight patients showed a positive result for HBV core antibodies (anti‐HBc) indicating a seroconversion of an acute HBV infection in the past. None of the anti‐HBc–negative patients were positive for anti‐HDV. Our results indicate a distinctly low probability of replicative HDV infection in HCV mono‐infected patients in Germany. Current German clinical guidelines rightly recommend performing HDV screening only in HBsAg‐positive patients. However, larger studies on this subject should be performed in regions that are endemic for chronic HBV/HDV as well as HCV infections. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Further characterization of bovine hepacivirus: Antibody response, course of infection, and host tropism.

Author

Baechlein, Christine, Baron, Anna Lena, Meyer, Denise, Gorriz‐Martin, Lara, Pfankuche, Vanessa Maria, Baumgärtner, Wolfgang, Polywka, Susanne, Peine, Sven, Fischer, Nicole, Rehage, Jürgen, and Becher, Paul

Subjects
FLAVIVIRUSES, VIRUS phylogeny, ANTIBODY formation, IMMUNE response, HEPATITIS C virus
Abstract

Bovine hepacivirus (BovHepV) is a recently added member to the growing genus Hepacivirus within the family Flaviviridae. Animal hepaciviruses are rarely characterized so far. Apart from norway rat hepacivirus which represents a promising HCV surrogate model, only equine hepaciviruses have been studied to some extent. BovHepV has been initially identified in bovine samples and was shown to establish persistent infections in cattle. However, consequences of those chronic infections, humoral immune response and the possibility of an extended host spectrum have not been explored so far. Therefore, we here investigated (a) the presence of anti‐NS3‐antibodies and viral RNA in cattle herds in Germany, (b) the course of infection in cattle, and (c) the host tropism including zoonotic potential of bovine hepaciviruses. Our results show that 19.9% of investigated bovine serum samples had antibodies against BovHepV. In 8.2% of investigated samples, viral RNA was detected. Subsequent genetic analysis revealed a novel genetic cluster of BovHepV variants. For 25 selected cattle in a BovHepV positive herd the presence of viral genomic RNA was monitored over one year in two to three months intervals by RT‐PCR in order to discriminate acute versus persistent infection. In persistently infected animals, no serum antibodies were detected. Biochemical analyses could not establish a link between BovHepV infection and liver injury. Apart from a single sample of a pig providing a positive reaction in the antibody test, neither BovHepV‐specific antibodies nor viral RNA were detected in porcine, equine or human samples implying a strict host specificity of BovHepV. [ABSTRACT FROM AUTHOR]

Published
2019
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26. Human liver chimeric mice as a new model of chronic hepatitis E virus infection and preclinical drug evaluation

Author

Allweiss, Lena, primary, Gass, Sofia, additional, Giersch, Katja, additional, Groth, Anne, additional, Kah, Janine, additional, Volz, Tassilo, additional, Rapp, Gianna, additional, Schöbel, Anja, additional, Lohse, Ansgar W., additional, Polywka, Susanne, additional, Pischke, Sven, additional, Herker, Eva, additional, Dandri, Maura, additional, and Lütgehetmann, Marc, additional

Published
2016
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29. Persistent hepatitis D virus mono-infection in humanized mice is efficiently converted by hepatitis B virus to a productive co-infection

Author

Giersch, Katja, primary, Helbig, Martina, additional, Volz, Tassilo, additional, Allweiss, Lena, additional, Mancke, Lida V., additional, Lohse, Ansgar W., additional, Polywka, Susanne, additional, Pollok, Jörg M., additional, Petersen, Jörg, additional, Taylor, John, additional, Dandri, Maura, additional, and Lütgehetmann, Marc, additional

Published
2014
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30. The efficiency of humoral immune transfer depends on both the graft and the immunosuppressive treatment

Author

Dahmen, Uta, Li, Jun, Gu, Yi, Doebel, Lothar, Fan, Li Ming, Polywka, Susanne, Dirsch, Olaf, and Broelsch, Christoph E

Subjects
Transplantation, Medizin, Intercellular Adhesion Molecule-1, Immunohistochemistry, Kidney Transplantation, Liver Transplantation, Rats, Rats, Inbred ACI, Rats, Inbred Lew, Transplantation Immunology, Rats, Inbred BN, Antibody Formation, Animals, Heart Transplantation, Transplantation, Homologous, Immunosuppressive Agents
Abstract

The present study was designed to compare the efficiency of adoptive transfer of humoral immunity after liver, kidney, and heart transplantation in relation to the number of passenger lymphocytes, and to estimate the risk of a detrimental effect and the chance of a beneficial effect. Hepatitis B virus surface-antigen-vaccinated brown Norway rats (BNs) and AxC 9935 Irish (ACI rats) served as donors, and naïve Lewis (LEW) rats as recipients. The liver grafts contained 100 times more passenger lymphocytes than heart grafts, and the kidney grafts approximately ten times more, indicated by monoclonal CD45 antibody staining. Transient anti-HBs immunity did occur after transplantation of all three organ grafts. In all rejecting groups, the serum recipient-to-donor anti-HBs titer ratio (R/D ratio) was below 0.10%, with heart recipients showing half the level (0.05%) of liver recipients (0.09%). Under immunosuppression, R/D ratio doubled in liver or kidney recipients, but remained unaffected in heart recipients. Immune transfer was most efficient in immune-suppressed liver recipients in the spontaneously tolerant strain combination as indicated by a significantly higher R/D ratio (0.32%) and a longer titer persistence (up to 9 weeks) than in all other groups. Therefore, mainly liver and kidney graft recipients carry a risk, but also a chance of benefiting from the transfer of donor-derived immunity.

Published
2001

36. Inter‐laboratory comparison of HCV‐RNA assay results: Implications for multi‐centre research

Author

Pembrey, Lucy, primary, Newell, Marie‐Louise, additional, Tovo, Pier‐Angelo, additional, van Drimmelen, Harry, additional, Quinti, Isabella, additional, Furlini, Giuliano, additional, Galli, Silvia, additional, Meliconi, Maria Grazia, additional, Burns, Sheila, additional, Hallam, Nick, additional, Sönnerborg, Anders, additional, Cilla, Gustavo, additional, Serrano, Esther, additional, Laccetti, Paolo, additional, Portella, Giuseppe, additional, Polywka, Susanne, additional, Icardi, Giancarlo, additional, Bruzzone, Bianca, additional, Balbo, Luciano, additional, and Alfarano, Alda, additional

Published
2002
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39. A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay.

Author

Walker, John M., Zhu, Tuofu, Polywka, Susanne, Duttmann, Hedwig, Lübben, Frank, Laufs, Rainer, and Feldner, Jürgen

Abstract

It is important to shorten the window period after acute HIV infection in which infected individuals are still antibody-negative, especially in blood donors. Newly developed fourth-generation assays detect antibodies to HIV-1, including subtype O, and to HIV-2 and, simultaneously, p24 antigen of HIV-1. To evaluate this assay for daily routine work we compared it with different third-generation assays using sera from uninfected patients and patients with known HIV infection. The most interesting sera are those drawn during seroconversion from freshly infected patients. Whenever we encounter such a patient with acute HIV infection we store the serum in aliquots at −20°C. Thus, we were able to establish our own seroconversion panel and use it in our laboratory for evaluation of new assays. The new test was shown to be able to detect all chronically HIV-infected individuals and four of six patients during seroconversion although in two of these patients conventional assays for HIV antibodies were still negative. The rate of unspecific reactivities was slightly higher as compared with third-generation assays. [ABSTRACT FROM AUTHOR]

Published
2005
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49. Virus NAT for HIV, HBV, and HCV in Post-Mortal Blood Specimens over 48 h after Death of Infected Patients - First Results.

Author

Meyer, Thomas, Polywka, Susanne, Wulff, Birgit, Edler, Carolin, Schröder, Ann Sophie, Wilkemeyer, Ina, Kalus, Ulrich, and Pruss, Axel

Subjects
*ORGAN donation, *AUTOPSY, *BLOOD microbiology, *BLOOD testing, *VIRUS diseases, *DEATH
Abstract

Objective: According to EU regulations (EU directive 2006/17/EC), blood specimens for virologic testing in the context of post-mortal tissue donation must be taken not later than 24 h post mortem. Methods: To verify validity of NAT in blood specimens collected later, viral nucleic acid concentrations were monitored in blood samples of deceased persons infected with HIV (n = 7), HBV (n = 5), and HCV (n = 17) taken upon admission and at 12 h, 24 h, 36 h and 48 h post mortem. HIV and HCV RNA were quantified using Cobas TaqMan (Roche), HBV DNA was measured by in-house PCR. Results: A more than 10-fold decrease of viral load in samples taken 36 h or 48 h post mortem was seen in one HIV-infected patient only. For all other patients tested the decrease of viral load in 36hour or 48-hour post-mortal samples was less pronounced. Specimens of 3 HIV- and 2 HBV-infected patients taken 24 h post mortem or later were even found to have increased concentrations (>10-fold), possibly due to post-mortem liberation of virus from particular cells or tissues. Conclusion: Our preliminary data indicate that the time point of blood collection for HIV, HBV and HCV testing by PCR may be extended to 36 h or even 48 h post mortem and thus improve availability of tissue donations. [ABSTRACT FROM AUTHOR]

Published
2012
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50. Inter-laboratory comparison of HCV-RNA assay results: Implications for multi-centre research.

Author

Pembrey, Lucy, Newell, Marie-Louise, Tovo, Pier-Angelo, van Drimmelen, Harry, Quinti, Isabella, Furlini, Giuliano, Galli, Silvia, Meliconi, Maria Grazia, Burns, Sheila, Hallam, Nick, Sönnerborg, Anders, Cilla, Gustavo, Serrano, Esther, Laccetti, Paolo, Portella, Giuseppe, Polywka, Susanne, Icardi, Giancarlo, Bruzzone, Bianca, Balbo, Luciano, and Alfarano, Alda

Published
2003
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