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1. Co-delivery of amphipol-conjugated adjuvant with antigen, and adjuvant combinations, enhance immune protection elicited by a membrane protein-based vaccine against a mucosal challenge with Chlamydia

Author: Tifrea, Delia F, Pal, Sukumar, Le Bon, Christel, Giusti, Fabrice, Popot, Jean-Luc, Cocco, Melanie J, Zoonens, Manuela, and de la Maza, Luis M
Subjects: Biomedical and Clinical Sciences, Immunization, Vaccine Related, Infectious Diseases, Prevention, 3.4 Vaccines, Infection, Adjuvants, Immunologic, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Chlamydia Infections, Chlamydia muridarum, Female, Mice, Mice, Inbred BALB C, Chlamydia, Vaccine, Amphipols, A8-35, Adjuvant, EP-67, MOMP, Biological Sciences, Agricultural and Veterinary Sciences, Medical and Health Sciences, Virology, Biomedical and clinical sciences, Health sciences
Abstract: IntroductionChlamydial infections are spread worldwide and a vaccine is needed to control this pathogen. The goals of this study were to determine if the delivery of an adjuvant associated to the antigen, via a derivatized amphipol, and adjuvant combinations improve vaccine protection.MethodsA novel approach, trapping the Chlamydia muridarum (Cm) native MOMP (nMOMP) with amphipols (A8-35), bearing a covalently conjugated peptide (EP67), was used. Adjuvants incorporated were: EP67 either conjugated to A8-35, which was used to trap nMOMP (nMOMP/EP67-A8-35), or free as a control, added to nMOMP/A8-35 complexes (nMOMP/A8-35+EP67); Montanide ISA 720 to enhance humoral responses, and CpG-1826 to elicit robust cell-mediated immunity (CMI). BALB/c mice were immunized by mucosal and systemic routes. Intranasal immunization with live Cm was used as positive control and three negative controls were included. Mice were challenged intranasally with Cm and changes in body weight, lungs weight and number of Cm-inclusion forming units (IFU) recovered from the lungs were evaluated to establish protection. To assess local responses levels of IFN- γ and Cm-specific IgA were determined in lungs' supernatants.ResultsStructural assays demonstrated that nMOMP secondary structure and thermal stability were maintained when A8-35 was covalently modified. Mice vaccinated with nMOMP/EP67-A8-35 were better protected than animals immunized with nMOMP/A8-35+EP67. Addition of Montanide enhanced Th2 responses and improved protection. Including CpG-1826 further broadened, intensified and switched to Th1-biased immune responses. With delivery of nMOMP and the three adjuvants, as determined by changes in body weight, lungs weight and number of IFU recovered from lungs, protection at 10 days post-challenge was equivalent to that induced by immunization with live Cm.ConclusionsCovalent association of EP67 to A8-35, used to keep nMOMP water-soluble, improves protection over that conferred by free EP67. Adjuvant combinations including EP67+Montanide+CpG-1826, by broadening and intensifying cellular and humoral immune responses, further enhanced protection.
Published: 2018

2. Optical Spectroscopy of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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3. Solution Studies of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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4. Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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5. Amphipol-Assisted Cell-Free Expression of Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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6. Amphipol-Assisted Folding of Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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7. Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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8. Formation and Properties of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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9. Membrane Proteins and Their Natural Environment

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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10. Extracting Membrane Proteins from Their Native Environment

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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11. Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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12. Biomedical Applications

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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13. The Use of Amphipols in Mass Spectrometry

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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14. The Use of Amphipols for Electron Microscopy

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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15. Amphipols and Membrane Protein Crystallization

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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16. Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
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17. Long-Term Stability of a Vaccine Formulated with the Amphipol-Trapped Major Outer Membrane Protein from Chlamydia trachomatis

Author: Eric Feinstein, H, Tifrea, Delia, Sun, Guifeng, Popot, Jean-Luc, de la Maza, Luis M, and Cocco, Melanie J
Subjects: Biochemistry and Cell Biology, Biological Sciences, Infectious Diseases, Immunization, Biotechnology, Sexually Transmitted Infections, Prevention, Vaccine Related, 3.4 Vaccines, Infection, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Chemistry, Pharmaceutical, Chlamydia trachomatis, Drug Carriers, Drug Evaluation, Preclinical, Drug Stability, Hydrophobic and Hydrophilic Interactions, Protein Conformation, Protein Denaturation, Solubility, Surface-Active Agents, Amphipols, Vaccine, Stability, MOMP, NMR, Microbiology, Medical Microbiology, Physiology, Biochemistry and cell biology
Abstract: Chlamydia trachomatis is a major bacterial pathogen throughout the world. Although antibiotic therapy can be implemented in the case of early detection, a majority of the infections are asymptomatic, requiring the development of preventive measures. Efforts have focused on the production of a vaccine using the C. trachomatis major outer membrane protein (MOMP). MOMP is purified in its native (n) trimeric form using the zwitterionic detergent Z3-14, but its stability in detergent solutions is limited. Amphipols (APols) are synthetic polymers that can stabilize membrane proteins (MPs) in detergent-free aqueous solutions. Preservation of protein structure and optimization of exposure of the most effective antigenic regions can avoid vaccination with misfolded, poorly protective protein. Previously, we showed that APols maintain nMOMP secondary structure and that nMOMP/APol vaccine formulations elicit better protection than formulations using either recombinant or nMOMP solubilized in Z3-14. To achieve a greater understanding of the structural behavior and stability of nMOMP in APols, we have used several spectroscopic techniques to characterize its secondary structure (circular dichroism), tertiary and quaternary structures (immunochemistry and gel electrophoresis) and aggregation state (light scattering) as a function of temperature and time. We have also recorded NMR spectra of (15)N-labeled nMOMP and find that the exposed loops are detectable in APols but not in detergent. Our analyses show that APols protect nMOMP much better than Z3-14 against denaturation due to continuous heating, repeated freeze/thaw cycles, or extended storage at room temperature. These results indicate that APols can help improve MP-based vaccine formulations.
Published: 2014

18. Increased Immunoaccessibility of MOMP Epitopes in a Vaccine Formulated with Amphipols May Account for the Very Robust Protection Elicited against a Vaginal Challenge with Chlamydia muridarum

Author: Tifrea, Delia F, Pal, Sukumar, Popot, Jean-Luc, Cocco, Melanie J, and de la Maza, Luis M
Subjects: Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Vaccine Related, Infectious Diseases, Immunization, Sexually Transmitted Infections, Prevention, 3.4 Vaccines, Infection, Good Health and Well Being, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Bacterial Vaccines, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Chlamydia Infections, Chlamydia muridarum, Female, Mice, Mice, Inbred BALB C, Pregnancy, Propylamines, Biochemistry and cell biology
Abstract: There is a need to implement a vaccine to protect against Chlamydia trachomatis infections. To test a new vaccine, mice were immunized with the Chlamydia muridarum native major outer membrane protein (nMOMP) solubilized with either amphipol A8-35 or the detergent Z3-14. OVA was used as a negative control, and mice were inoculated intranasally with C. muridarum as positive controls. Animals vaccinated with nMOMP mounted strong Chlamydia-specific humoral and cell-mediated immune responses. Mice vaccinated with nMOMP/A8-35 had a higher ratio of Abs to denatured elementary bodies (EB) over live EB, recognized more synthetic MOMP peptides and had higher neutralizing titers than sera from mice immunized with nMOMP/Z3-14. T cell lymphoproliferative responses and levels of IFN-γ were also higher in mice vaccinated with nMOMP/A8-35 than with nMOMP/Z3-14. Following immunization, animals were challenged intravaginally with C. muridarum. On the basis of the number of mice with positive vaginal cultures, length of vaginal shedding, total number of positive vaginal cultures, and number of Chlamydia inclusion forming units recovered, nMOMP/A8-35 elicited a more robust protection than nMOMP/Z3-14. By depleting T cells with Abs, we determined that CD4(+) and not CD8(+) T cells mediated the protection elicited by nMOMP/A8-35. Mice were subsequently mated, and based on the number of pregnant mice and number of embryos, animals that were vaccinated with nMOMP/A8-35 or nMOMP/Z3-14 had fertility rates equivalent to the positive control group immunized with live EB and the fertility controls. In conclusion, increased accessibility of epitopes in the nMOMP/A8-35 preparation may account for the very robust protection against infection and disease elicited by this vaccine.
Published: 2014

19. BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy

Author: Perry, Thomas Noe, Souabni, Hager, Rapisarda, Chiara, Fronzes, Rémi, Giusti, Fabrice, Popot, Jean-Luc, Zoonens, Manuela, and Gubellini, Francesca
Published: 2019
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20. La structure des protéines

Author: Yon-Kahn, Jeannine, primary, Hervé, Guy, additional, and Popot, Jean-Luc, additional
Published: 2020
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21. Folding and stabilizing membrane proteins in amphipol A8-35

Author: Le Bon, Christel, Marconnet, Anaïs, Masscheleyn, Sandrine, Popot, Jean-Luc, and Zoonens, Manuela
Published: 2018
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22. NMR Study of a Membrane Protein in Detergent-Free Aqueous Solution

Author: Zoonens, Manuela, Catoire, Laurent J., Giusti, Fabrice, Popot, Jean-Luc, and Engelman, Donald M.
Published: 2005

23. Amphipols: Polymers that Keep Membrane Proteins Soluble in Aqueous Solutions

Author: Tribet, Christophe, Audebert, Roland, and Popot, Jean-Luc
Published: 1996

24. Amphipols: A General Introduction and Some Protocols

Author: Zoonens, Manuela, Zito, Francesca, Martinez, Karen L., Popot, Jean-Luc, and Mus-Veteau, Isabelle, editor
Published: 2014
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25. Folding and stability of integral membrane proteins in amphipols

Author: Kleinschmidt, Jörg H. and Popot, Jean-Luc
Published: 2014
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26. Folding membrane proteins in vitro: A table and some comments

Author: Popot, Jean-Luc
Published: 2014
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27. Extracting Membrane Proteins from Their Native Environment

Author: Popot, Jean-Luc, primary
Published: 2018
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28. Amphipol-Assisted Cell-Free Expression of Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
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29. Biomedical Applications

Author: Popot, Jean-Luc, primary
Published: 2018
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30. The Use of Amphipols for Electron Microscopy

Author: Popot, Jean-Luc, primary
Published: 2018
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31. Amphipols and Membrane Protein Crystallization

Author: Popot, Jean-Luc, primary
Published: 2018
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32. Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, primary
Published: 2018
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33. Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications

Author: Popot, Jean-Luc, primary
Published: 2018
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34. Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
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35. Optical Spectroscopy of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
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36. Formation and Properties of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
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37. Membrane Proteins and Their Natural Environment

Author: Popot, Jean-Luc, primary
Published: 2018
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38. Amphipol-Assisted Folding of Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
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39. Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols

Author: Popot, Jean-Luc, primary
Published: 2018
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40. Solution Studies of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
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41. The Use of Amphipols in Mass Spectrometry

Author: Popot, Jean-Luc, primary
Published: 2018
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42. Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, primary
Published: 2018
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43. Folding of diphtheria toxin T-domain in the presence of amphipols and fluorinated surfactants: Toward thermodynamic measurements of membrane protein folding

Author: Kyrychenko, Alexander, Rodnin, Mykola V., Vargas-Uribe, Mauricio, Sharma, Shivaji K., Durand, Grégory, Pucci, Bernard, Popot, Jean-Luc, and Ladokhin, Alexey S.
Published: 2012
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44. The Use of Amphipols as Universal Molecular Adapters to Immobilize Membrane Proteins onto Solid Supports

Author: Charvolin, Delphine, Perez, Jean-Baptiste, Rouvière, Florent, Giusti, Fabrice, Bazzacco, Paola, Abdine, Alaa, Rappaport, Fabrice, Martinez, Karen L., and Popot, Jean-Luc
Published: 2009
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45. Amphipols stabilize the Chlamydia major outer membrane protein and enhance its protective ability as a vaccine

Author: Tifrea, Delia F., Sun, Guifeng, Pal, Sukumar, Zardeneta, Gustavo, Cocco, Melanie J., Popot, Jean-Luc, and de la Maza, Luis M.
Published: 2011
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