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23 results on '"Porea, Daniela"'

1. Evidence of West Nile Virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016

Author

Vasic, Ana, Oslobanu, Luanda Elena, Marinov, Mihai, Crivei, Luciana Alexandra, Ratoi, Ioana Alexandra, Anita, Adriana, Anita, Dragos, Dorosencu, Alexandru, Alexe, Vasile, Raileanu, Stefan, Simeunovic, Predrag, Raileanu, Cristian, Falcuta, Elena, Prioteasa, Florian Liviu, Bojkovski, Jovan, Pavlovic, Ivan, Mathis, Alexander, Tews, Birke Andrea, Savuta, Gheorghe, Veronesi, Eva, Silaghi, Cornelia, Cimpan, Andrei, Ciuca, Lavinia, Cojkic, Aleksandar, Draskovic, Vladimir, Djuric, Miloje, Glavinic, Uros, Ivanescu, Maria Larisa, Kavran, Mihaela, Lupu, Andrei, Mindru, Raluca, Porea, Daniela, Radanovic, Oliver, Ristanic, Marko, Roman, Constantin, Stanisic, Ljubodrag, Zdravkovic, Nemanja, and Vaselek, Slavica

Published
2019

4. Detection of west nile virus lineage 2 in eastern romania and first identification of sindbis virus rna in mosquitoes analyzed using high-throughput microfluidic real-time PCR

Author

Crivei, Luciana Alexandra, Moutailler, Sara, Gonzalez, Gaëlle, Lowenski, Steeve, Crivei, Ioana Cristina, Porea, Daniela, Anita, Dragos Constantin, Ratoi, Ioana Alexandra, Zientara, Stéphan, Oslobanu, Luanda Elena, Tomazotos, Alexandru, Savuta, Gheorghe, Lecollinet, Sylvie, Crivei, Luciana Alexandra, Moutailler, Sara, Gonzalez, Gaëlle, Lowenski, Steeve, Crivei, Ioana Cristina, Porea, Daniela, Anita, Dragos Constantin, Ratoi, Ioana Alexandra, Zientara, Stéphan, Oslobanu, Luanda Elena, Tomazotos, Alexandru, Savuta, Gheorghe, and Lecollinet, Sylvie

Abstract

The impact of mosquito-borne diseases on human and veterinary health is being exacerbated by rapid environmental changes caused mainly by changing climatic patterns and globalization. To gain insight into mosquito-borne virus circulation from two counties in eastern and southeastern Romania, we have used a combination of sampling methods in natural, urban and peri-urban sites. The presence of 37 mosquito-borne viruses in 16,827 pooled mosquitoes was analyzed using a high-throughput microfluidic real-time PCR assay. West Nile virus (WNV) was detected in 10/365 pools of Culex pipiens (n = 8), Culex modestus (n = 1) and Aedes vexans (n = 1) from both studied counties. We also report the first molecular detection of Sindbis virus (SINV) RNA in the country in one pool of Culex modestus. WNV infection was confirmed by real-time RT-PCR (10/10) and virus isolation on Vero or C6/36 cells (four samples). For the SINV-positive pool, no cytopathic effectwas observed after infection of Vero or C6/36 cells, but no amplification was obtained in conventional SINV RT-PCR. Phylogenetic analysis of WNV partial NS5 sequences revealed that WNV lineage 2 of the Central-Southeast European clade, has a wider circulation in Romania than previously known.

Published
2023

5. Detection of West Nile Virus Lineage 2 in Eastern Romania and First Identification of Sindbis Virus RNA in Mosquitoes Analyzed using High-Throughput Microfluidic Real-Time PCR

Author

CRIVEI, Luciana Alexandra, primary, MOUTAILLER, Sara, additional, GONZALEZ, Gaëlle, additional, LOWENSKI, Steeve, additional, CRIVEI, Ioana Cristina, additional, POREA, Daniela, additional, ANITA, Dragoș Constantin, additional, RATOI, Ioana Alexandra, additional, ZIENTARA, Stéphan, additional, OSLOBANU, Luanda Elena, additional, TOMAZATOS, Alexandru, additional, SAVUTA, Gheorghe, additional, and LECOLLINET, Sylvie, additional

Published
2023
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7. Genetic population structure of the critically endangered stellate sturgeon (Acipenser stellatus) in the Black Sea basin: Implications for conservation

Author

Holostenco, Daniela Nicoleta, primary, Ciorpac, Mitică, additional, Firidin, Șirin, additional, Eroglu, Oguzhan, additional, Memiș, Devrim, additional, Paraschiv, Marian, additional, Honț, Ștefan, additional, Iani, Marian, additional, Tošić, Katarina, additional, Taflan, Elena, additional, Porea, Daniela, additional, Kersten, Petra, additional, Aydın, İlhan, additional, Suciu, Radu, additional, Gessner, Jörn, additional, Rîșnoveanu, Geta, additional, and Kohlmann, Klaus, additional

Published
2022
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8. SEROLOGICAL SCREENING FOR Neospora caninum INFECTION IN A DAIRY CATTLE FARM WITH REPRODUCTIVE DISORDERS, IN SOUTH-EASTERN ROMANIA.

Author

GOTU, Vasilica, MITREA, Emanuel, POREA, Daniela, IONITA, Mariana, and MITREA, Ioan Liviu

Abstract

Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67-37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32 - 58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Common European Origin of Hepatitis E Virus in Human Population From Eastern Romania

Author

Porea, Daniela, primary, Anita, Adriana, additional, Vata, Andrei, additional, Teodor, Danut, additional, Crivei, Luciana, additional, Raileanu, Cristian, additional, Gotu, Vasilica, additional, Ratoi, Ioana, additional, Cozma, Andreea, additional, Anita, Dragos, additional, Oslobanu, Luanda, additional, Pavio, Nicole, additional, and Savuta, Gheorghe, additional

Published
2020
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14. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia

Author

Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, University of Zurich, and Silaghi, Cornelia

Subjects
10078 Institute of Parasitology, 0301 basic medicine, Entomology, Ceratopogonidae, 030231 tropical medicine, 2405 Parasitology, Biodiversity, Zoology, Capacity building, 610 Medicine & health, Biology, Real-Time Polymerase Chain Reaction, Arbovirus, Host Specificity, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, 600 Technology, medicine, Animals, lcsh:RC109-216, SBV, Host (biology), Research, Species diversity, Schmallenberg virus, 2725 Infectious Diseases, Feeding Behavior, 15. Life on land, biology.organism_classification, Culicoides, medicine.disease, Train the trainers concept, Insect Vectors, 030104 developmental biology, Infectious Diseases, Host preference, Culicoides spp, 570 Life sciences, biology, Parasitology, Serbia, Arboviruses, BTV
Abstract

Background Culicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as “no-see ums”) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia. Results In total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, “Others” group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative. Conclusions The biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project “AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges” funded by the programme SCOPES of the Swiss National Science Foundation.

Published
2019

15. Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016

Author

Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, Cristian, Simeunović, Predrag, Falcuța, E., Prioteasa, F.L., Bojkovski, Jovan, Pavlović, I., Mathis, A., Tews, B.A., Savuta, Gheorghe, Veronesi, E., Silaghi, Cornelia, Cimpan, A., Ciucă, L., Cojkić, A., Drašković, Vladimir, Đurić, M., Glavinić, Uroš, Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, Raluca, Porea, Daniela, Radanović, Oliver, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Zdravković, Nemanja, Vaselek, S., SCOPES, AMSAR training group, Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, Cristian, Simeunović, Predrag, Falcuța, E., Prioteasa, F.L., Bojkovski, Jovan, Pavlović, I., Mathis, A., Tews, B.A., Savuta, Gheorghe, Veronesi, E., Silaghi, Cornelia, Cimpan, A., Ciucă, L., Cojkić, A., Drašković, Vladimir, Đurić, M., Glavinić, Uroš, Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, Raluca, Porea, Daniela, Radanović, Oliver, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Zdravković, Nemanja, Vaselek, S., and SCOPES, AMSAR training group

Abstract

West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.

Published
2019

23. Molecular Evidence of Rickettsia spp., Anaplasma phagocytophilum, and "Candidatus Neoehrlichia mikurensis" in Ticks from Natural and Urban Habitats in Eastern Romania.

Author

Raileanu C, Moutailler S, Porea D, Oslobanu L, Anita D, Anita A, Vayssier-Taussat M, and Savuta G

Subjects
Animal Distribution, Animals, Cities, Ecosystem, Humans, Romania, Anaplasma phagocytophilum isolation & purification, Ixodidae microbiology, Rickettsia isolation & purification
Abstract

Ixodid ticks are competent vectors for multiple pathogens, several of which cause infections in human. The medical importance of tick-borne pathogens is well known, yet unanswered questions remain regarding the occurrence of pathogens such as Rickettsia spp., Anaplasma phagocytophilum, and "Candidatus Neoehrlichia mikurensis" in questing ticks in Romania. Our objectives were to identify three emerging tick-borne zoonotic pathogens in eastern Romania, to assess their prevalence, establish co-infection rates, and to compare infection levels of selected pathogens in questing ticks collected from one suburban area in the city of Iaşi and one forested area located in the Danube Delta Biosphere Reserve. We collected 490 questing nymphs or adult ticks (467 Ixodes ricinus, 4 Dermacentor reticulatus, and 19 Haemaphysalis punctata). We individually analyzed ticks for the presence of Rickettsia spp., A. phagocytophilum, and "C. N. mikurensis." Rickettsia spp. was detected in 9.4% of ticks from both sampling areas. Rickettsia spp. included R. helvetica (n = 17 I. ricinus ticks), R. monacensis (n = 28 I. ricinus ticks), and R. raoultii (n = 1 D. reticulatus). "C. N. mikurensis" had an infection rate of 4.9% while A. phagocytophilum was detected only in the forested area with a global prevalence of 1.2%. The overall prevalence of ticks infected with at least one pathogen was 15.5%, and 5.3% of infected ticks were tested positives for dual pathogen association. Our study documents the presence of pathogens in questing ticks in the urban recreational areas of Iaşi and forested areas located in the Danube Delta Biosphere Reserve. Worth mentioning, is the presence of "C. N. mikurensis" in ticks from eastern Romania, an agent just recently described in Romania, and the existence of co-infections in ticks at a similar prevalence to other European countries.

Published
2018
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