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1. Personalized Antibiotic Therapy for the Critically Ill: Implementation Strategies and Effects on Clinical Outcome of Piperacillin Therapeutic Drug Monitoring—A Descriptive Retrospective Analysis

Author

Schrader Nikolas, Riese Thorsten, Kurlbaum Max, Meybohm Patrick, Kredel Markus, Surat Güzin, Scherf-Clavel Oliver, Strate Alexander, Pospiech Andreas, and Hoppe Kerstin

Subjects
therapeutic drug monitoring, piperacillin/tazobactam, personalized antimicrobial therapy, antimicrobial stewardship, Therapeutics. Pharmacology, RM1-950
Abstract

Therapeutic drug monitoring (TDM) is increasingly relevant for an individualized antibiotic therapy and subsequently a necessary tool to reduce multidrug-resistant pathogens, especially in light of diminishing antimicrobial capabilities. Critical illness is associated with profound pharmacokinetic and pharmacodynamic alterations, which challenge dose finding and the application of particularly hydrophilic drugs such as β-lactam antibiotics. Methods: Implementation strategy, potential benefit, and practicability of the developed standard operating procedures were retrospectively analyzed from January to December 2020. Furthermore, the efficacy of the proposed dosing target of piperacillin in critically ill patients was evaluated. Results: In total, 160 patients received piperacillin/tazobactam therapy and were subsequently included in the study. Of them, 114 patients received piperacillin/tazobactam by continuous infusion and had at least one measurement of piperacillin serum level according to the standard operating procedure. In total, 271 measurements were performed with an average level of 79.0 ± 46.0 mg/L. Seventy-one piperacillin levels exceeded 100 mg/L and six levels were lower than 22.5 mg/L. The high-level and the low-level group differed significantly in infection laboratory parameters (CRP (mg/dL) 20.18 ± 11.71 vs. 5.75 ± 5.33) and renal function [glomerular filtration rate (mL/min/1.75 m2) 40.85 ± 26.74 vs. 120.50 ± 70.48]. Conclusions: Piperacillin levels are unpredictable in critically ill patients. TDM during piperacillin/tazobactam therapy is highly recommended for all patients. Although our implementation strategy was effective, further strategies implemented into the daily clinical workflow might support the health care staff and increase the clinicians’ alertness.

Published
2021
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2. Smooth trends in fermium charge radii and the impact of shell effects

Author

Warbinek, Jessica, Rickert, Elisabeth, Raeder, Sebastian, Albrecht-Schönzart, Thomas, Andelic, Brankica, Auler, Julian, Bally, Benjamin, Bender, Michael, Berndt, Sebastian, Block, Michael, Brizard, Alexandre, Chauveau, Pierre, Cheal, Bradley, Chhetri, Premaditya, Claessens, Arno, de Roubin, Antoine, Devlin, Charlie, Dorrer, Holger, Düllmann, Christoph E., Ezold, Julie, Ferrer, Rafael, Gadelshin, Vadim, Gaiser, Alyssa, Giacoppo, Francesca, Goriely, Stephane, Gutiérrez, Manuel J., Harvey, Ashley, Hasse, Raphael, Heinke, Reinhard, Heßberger, Fritz-Peter, Hilaire, Stephane, Kaja, Magdalena, Kaleja, Oliver, Kieck, Tom, Kim, EunKang, Kneip, Nina, Köster, Ulli, Kraemer, Sandro, Laatiaoui, Mustapha, Lantis, Jeremy, Lecesne, Nathalie, Loria Basto, Andrea Tzeitel, Mistry, Andrew Kishor, Mokry, Christoph, Moore, Iain, Murböck, Tobias, Münzberg, Danny, Nazarewicz, Witold, Niemeyer, Thorben, Nothhelfer, Steven, Péru, Sophie, Raggio, Andrea, Reinhard, Paul-Gerhard, Renisch, Dennis, Rey-Herme, Emmanuel, Romans, Jekabs, Romero Romero, Elisa, Runke, Jörg, Ryssens, Wouter, Savajols, Hervé, Schneider, Fabian, Sperling, Joseph, Stemmler, Matou, Studer, Dominik, Thörle-Pospiech, Petra, Trautmann, Norbert, Urquiza-González, Mitzi, van Beek, Kenneth, Van Cleve, Shelley, Van Duppen, Piet, Vandebrouck, Marine, Verstraelen, Elise, Walther, Thomas, Weber, Felix, and Wendt, Klaus

Published
2024
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4. The core of secondary level quantum education: a multi-stakeholder perspective

Author

Merzel, Avraham, Bitzenbauer, Philipp, Krijtenburg-Lewerissa, Kim, Stadermann, Kirsten, Andreotti, Erica, Anttila, Daria, Bondani, Maria, Chiofalo, Maria Luisa (Marilù), Faletič, Sergej, Frans, Renaat, Goorney, Simon, Greinert, Franziska, Jurčić, Leon, Koupilová, Zdeňka, Malgieri, Massimiliano, Müller, Rainer, Onorato, Pasquale, Pospiech, Gesche, Ubben, Malte, Woitzik, Andreas, and Pol, Henk

Published
2024
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5. Contributions from Pilot Projects in Quantum Technology Education as Support Action to Quantum Flagship

Author

Faletic, Sergej, Bitzenbauer, Philipp, Bondani, Maria, Chiofalo, Marilu, Goorney, Simon, Krijtenburg-Lewerissa, Kim, Mishina, Oxana, Muller, Rainer, Pospiech, Gesche, Ercan, Ilke, Malgieri, Massimiliano, Merzel, Avraham, Michelini, Marisa, Onorato, Pasquale, Pol, Henk, Santi, Lorenzo, Seskir, Zeki Can, Sherson, Jacob, Stadermann, Kirsten, Stefanel, Alberto, Surer, Elif, Toth, Kristof, Malo, Jorge Yago, and Zabello, Olgas

Subjects
Physics - Physics Education, Quantum Physics
Abstract

The GIREP community on teaching and learning quantum physics and the Education section of the Quantum flagship project of the European Union (QTEdu) have brought together different stakeholders in the field of teaching quantum physics on all levels, including outreach. The goal of QTEdu is to pave the way for the training of the future quantum workforce. To this end, it is necessary to understand the needs of the quantum technology (QT) field, make the general public aware of the existence and importance of QT, and introduce quantum physics already in high school, so that high school students can choose QT as their field of study and career. Finally, new university courses need to be established to support emerging specific profiles such as a quantum engineer. In this symposium, four QTEdu pilot projects were brought together to demonstrate how their complementary approaches have worked towards realising the above goals., Comment: 14 pages, 4 figures

Published
2023

6. The Influence of the Composition of Pulp and the Number of Layers on the Strength Properties of Multi-Layer Papers Intended for the Production of Corrugated Paperboard

Author

Karolina Radomska, Konrad Olejnik, and Piotr Pospiech

Subjects
paper, multi-ply papers, paper strength properties, papermaking potential, corrugated board, recycled fibers, Biotechnology, TP248.13-248.65
Abstract

This research investigated the influence of the layered structure of paper on its selected strength properties. Two types of cellulose pulp were used: unbleached kraft pine pulp (UBSK) and unrefined bleached birch kraft pulp (BHK). Multi-ply papers were obtained from layers of appropriate grammage formed separately and bonded in a wet state. Paper layers were formed from primary pulps (UBSK and BHK) or their mixtures (three-ply UBSK-BHK paper and three-ply UBSK-BHK-UBSK paper), from which various variants of laboratory paper samples were made. This work focused on three-layer papers. The obtained laboratory sheets were analysed for changes in tear resistance, tensile strength, bursting strength, short span compression strength (SCT), and corrugating medium test (CMT). In most cases, three-layer structures were characterised by higher strength parameters than single-layer papers. The tear strength and SCT of papers consisting of three layers formed from both mixed pulps were slightly lower than those of solid papers. The results demonstrate the feasibility of producing three-ply paper with significantly reduced variability in parameters such as elongation and bursting strength, which are inherently subject to high variability.

Published
2024

7. Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor traps.

Author

Remesh, Soumya G, Merz, Gregory E, Brilot, Axel F, Chio, Un Seng, Rizo, Alexandrea N, Pospiech, Thomas H, Lui, Irene, Laurie, Mathew T, Glasgow, Jeff, Le, Chau Q, Zhang, Yun, Diwanji, Devan, Hernandez, Evelyn, Lopez, Jocelyne, Mehmood, Hevatib, Pawar, Komal Ishwar, Pourmal, Sergei, Smith, Amber M, Zhou, Fengbo, QCRG Structural Biology Consortium, DeRisi, Joseph, Kortemme, Tanja, Rosenberg, Oren S, Glasgow, Anum, Leung, Kevin K, Wells, James A, and Verba, Kliment A

Subjects
QCRG Structural Biology Consortium, Humans, Antibodies, Monoclonal, Protein Binding, Antibodies, Neutralizing, COVID-19, Angiotensin-Converting Enzyme 2, SARS-CoV-2, ACE2 receptor traps, Rosetta, SARS-CoV-2 Omicron variant, Spike, cryo-EM, protein therapeutics, pseudovirus neutralization, Pneumonia, Lung, Biodefense, Vaccine Related, Prevention, Emerging Infectious Diseases, Pneumonia & Influenza, Infectious Diseases, Bioengineering, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, Chemical Sciences, Biological Sciences, Information and Computing Sciences, Biophysics
Abstract

The SARS-CoV-2 Omicron variant, with 15 mutations in Spike receptor-binding domain (Spike-RBD), renders virtually all clinical monoclonal antibodies against WT SARS-CoV-2 ineffective. We recently engineered the SARS-CoV-2 host entry receptor, ACE2, to tightly bind WT-RBD and prevent viral entry into host cells ("receptor traps"). Here we determine cryo-EM structures of our receptor traps in complex with stabilized Spike ectodomain. We develop a multi-model pipeline combining Rosetta protein modeling software and cryo-EM to allow interface energy calculations even at limited resolution and identify interface side chains that allow for high-affinity interactions between our ACE2 receptor traps and Spike-RBD. Our structural analysis provides a mechanistic rationale for the high-affinity (0.53-4.2 nM) binding of our ACE2 receptor traps to Omicron-RBD confirmed with biolayer interferometry measurements. Finally, we show that ACE2 receptor traps potently neutralize Omicron and Delta pseudotyped viruses, providing alternative therapeutic routes to combat this evolving virus.

Published
2023

9. Contributions from Pilot Projects in Quantum Technology Education as Support Action to Quantum Flagship

Author

Faletic, S., Bitzenbauer, P., Bondani, M., Chiofalo, M., Goorney, S. R., Krijtenburg-Lewerissa, K., Mishina, O., Müller, R., Pospiech, G., Ercan, İ., Malgieri, M., Merzel, A., Michelini, M., Onorato, P., Pol, H., Santi, L., Seskir, Z. C., Sherson, J., Stadermann, H. K. E., Stefanel, A., Surer, E., Tóth, K., Yago Malo, J., Zabello, O., Michelini, Marisa, Series Editor, Faletič, Sergej, editor, and Pavlin, Jerneja, editor

Published
2024
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16. EURAD state-of-the-art report: development and improvement of numerical methods and tools for modeling coupled processes in the field of nuclear waste disposal

Author

F. Claret, N. I. Prasianakis, A. Baksay, D. Lukin, G. Pepin, E. Ahusborde, B. Amaziane, G. Bátor, D. Becker, A. Bednár, M. Béreš, S. Bérešová, Z. Böthi, V. Brendler, K. Brenner, J. Březina, F. Chave, S. V. Churakov, M. Hokr, D. Horák, D. Jacques, F. Jankovský, C. Kazymyrenko, T. Koudelka, T. Kovács, T. Krejčí, J. Kruis, E. Laloy, J. Landa, T. Ligurský, T. Lipping, C. López-Vázquez, R. Masson, J. C. L. Meeussen, M. Mollaali, A. Mon, L. Montenegro, B. Pisani, J. Poonoosamy, S. I. Pospiech, Z. Saâdi, J. Samper, A.-C. Samper-Pilar, G. Scaringi, S. Sysala, K. Yoshioka, Y. Yang, M. Zuna, and O. Kolditz

Subjects
numerical tools, numerical methods, upscaling, uncertainties treatment, machine learning, coupled processes, Plasma physics. Ionized gases, QC717.6-718.8, Nuclear and particle physics. Atomic energy. Radioactivity, QC770-798
Abstract

The Strategic Research Agenda (SRA; https://www.ejp-eurad.eu/publications/eurad-sra) of the European Joint Programme on Radioactive Waste Management (EURAD; https://www.ejp-eurad.eu/) describes the scientific and technical domains and sub-domains and knowledge management needs of common interest between EURAD participant organizations. Theme number 7 is entitled “Performance assessment, safety case development and safety analyses.” A list of research and development priorities and activities of common interest to be addressed within EURAD for theme 7 have been established. Amongst others, the Understanding and modelling of multi-physical Thermo-Hydro-Mechanical-Chemical coupled processes (THMC) occurring in radioactive waste disposal is a major and permanent issue to support optimization of design and safety case abstraction. To tackle this challenge a research work package entitled “DONUT: Development and improvement of numerical methods and tools for modelling coupled processes” has been conducted within the EURAD join programming initiative. The purpose of this work package is to improve/develop methods or numerical tools in order to go a step further in development of (i) relevant, performant and cutting-edge numerical methods that can easily be implemented in existing or new tools, in order to carry out high-performance computing to facilitate the study of highly coupled processes in large systems, (ii) numerical scale transition schemes for coupled processes, (iii) innovative numerical methods to carry out uncertainty and sensitivity analyses. In this paper the work carried out within the DONUT work package is put in perspective regarding the existing concept and literature on the field. It does not pretend to be exhaustive but rather to put emphasis on particular issues tackled during the project.

Published
2024
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17. Effective treatment of actinic keratosis on the hands with red light photodynamic therapy using BF-200 ALA

Author

Martina Ulrich, Uwe Reinhold, Rolf Dominicus, Roland Aschoff, Rolf-Markus Szeimies, Ruth Schäning, Marie-Theres Zeuner, Nicole Pospiech, and Thomas Dirschka

Subjects
Actinic keratosis, Hands, Acral, Photodynamic therapy, Aminolaevulinic acid, BF-Rhodo LED, Medicine (General), R5-920
Abstract

Background: Acral actinic keratosis (AK) lesions are considered difficult to treat, and published data for photodynamic therapy (PDT) on these lesions is limited. Thus, we evaluated sustained efficacy, safety, and satisfaction after PDT for AK on the hands. Methods: We analysed subgroup data for treatment on the hands from a randomised, double-blind, intra-individual phase III study. All participants previously underwent up to two field-directed red light PDTs with 10 % 5-aminolevulinic acid nanoemulsion gel (BF-200 ALA). Assessments included pain during PDT, clearance and recurrence rates, and satisfaction. Results: 24 participants treated on the hands were included; 21 participants were analysed. Complete clearance rates with BF-200 ALA were 90.9 % (lesion-based) and 76.2 % (per participant's side), both markedly higher than with vehicle. The lesion recurrence rate with BF-200 ALA was 29.0 %. Adverse events reflected the mode of action. Mean pain intensities were 4.8 ± 3.8 (BF-200 ALA) and 0.8 ± 2.1 (vehicle) on an 11-point numeric rating scale. Most participants (81.0 %) rated their satisfaction with BF-200 ALA as very good or good. Conclusion: This subgroup analysis indicates that PDT with BF-200 ALA provides a suitable treatment for AK lesions on the hands.

Published
2024
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18. Manifestation of relativistic effects in the chemical properties of nihonium and moscovium revealed by gas chromatography studies

Author

A. Yakushev, J. Khuyagbaatar, Ch. E. Düllmann, M. Block, R. A. Cantemir, D. M. Cox, D. Dietzel, F. Giacoppo, Y. Hrabar, M. Iliaš, E. Jäger, J. Krier, D. Krupp, N. Kurz, L. Lens, S. Löchner, Ch. Mokry, P. Mošať, V. Pershina, S. Raeder, D. Rudolph, J. Runke, L. G. Sarmiento, B. Schausten, U. Scherer, P. Thörle-Pospiech, N. Trautmann, M. Wegrzecki, and P. Wieczorek

Subjects
superheavy element chemistry, element 113–nihonium, element 115–moscovium, gas phase chromatography, recoil separators, Chemistry, QD1-999
Abstract

Chemical reactivity of the superheavy elements nihonium (Nh, element 113) and moscovium (Mc, element 115) has been studied by the gas-solid chromatography method using a new combined chromatography and detection setup. The Mc isotope, 288Mc, was produced in the nuclear fusion reaction of 48Ca ions with 243Am targets at the GSI Helmholtzzentrum Darmstadt, Germany. After isolating 288Mc ions in the gas-filled separator TASCA, adsorption of 288Mc and its decay product 284Nh on silicon oxide and gold surfaces was investigated. As a result of this work, the values of the adsorption enthalpy of Nh and Mc on the silicon oxide surface were determined for the first time, −∆HadsSiO2Mc=54−5+11 kJ/mol and −∆HadsSiO2Nh=58−3+8 kJ/mol (68% c.i.). The obtained −ΔHads values are in good agreement with results of advanced relativistic calculations. Both elements, Nh and Mc, were shown to interact more weakly with the silicon oxide surface than their lighter homologues Tl and Bi, respectively. However, Nh and Mc turned out to be more reactive than the neighbouring closed-shell and quasi-closed-shell elements copernicium (Cn, element 112) and flerovium (Fl, element 114), respectively. The established trend is explained by the influence of strong relativistic effects on the valence atomic orbitals of these elements.

Published
2024
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19. Sex dependence of postoperative pulmonary complications – A post hoc unmatched and matched analysis of LAS VEGAS

Author

Kroell, Wolfgang, Metzler, Helfried, Struber, Gerd, Wegscheider, Thomas, Gombotz, Hans, Hiesmayr, Michael, Schmid, Werner, Urbanek, Bernhard, Kahn, David, Momeni, Mona, Pospiech, Audrey, Lois, Fernande, Forget, Patrice, Grosu, Irina, Poelaert, Jan, van Mossevelde, Veerle, van Malderen, Marie-Claire, Dylst, Dimitri, van Melkebeek, Jeroen, Beran, Maud, de Hert, Stefan, De Baerdemaeker, Luc, Heyse, Bjorn, Van Limmen, Jurgen, Wyffels, Piet, Jacobs, Tom, Roels, Nathalie, De Bruyne, Ann, van de Velde, Stijn, Leva, Brigitte, Damster, Sandrine, Plichon, Benoit, Juros-Zovko, Marina, Djonoviċ-Omanoviċ, Dejana, Pernar, Selma, Zunic, Josip, Miskovic, Petar, Zilic, Antonio, Kvolik, Slavica, Ivic, Dubravka, Azenic-Venzera, Darija, Skiljic, Sonja, Vinkovic, Hrvoje, Oputric, Ivana, Juricic, Kazimir, Frkovic, Vedran, Kopic, Jasminka, Mirkovic, Ivan, Karanovic, Nenad, Carev, Mladen, Dropulic, Natasa, Saric, Jadranka Pavicic, Erceg, Gorjana, Dvorscak, Matea Bogdanovic, Mazul-Sunko, Branka, Pavicic, Anna Marija, Goranovic, Tanja, Maldini, Branka, Radocaj, Tomislav, Gavranovic, Zeljka, Mladic-Batinica, Inga, Sehovic, Mirna, Stourac, Petr, Harazim, Hana, Smekalova, Olga, Kosinova, Martina, Kolacek, Tomas, Hudacek, Kamil, Drab, Michal, Brujevic, Jan, Vitkova, Katerina, Jirmanova, Katerina, Volfova, Ivana, Dzurnakova, Paula, Liskova, Katarina, Dudas, Radovan, Filipsky, Radek, el Kafrawy, Samir, Abdelwahab, Hisham Hosny, Metwally, Tarek, Abdel-Razek, Ahmed, El-Shaarawy, Ahmed Mostafa, Hasan, Wael Fathy, Ahmed, Ahmed Gouda, Yassin, Hany, Magdy, Mohamed, Abdelhady, Mahdy, Mahran, Mohamed, Herodes, Eiko, Kivik, Peeter, Oganjan, Juri, Aun, Annika, Sormus, Alar, Sarapuu, Kaili, Mall, Merilin, Karjagin, Juri, Futier, Emmanuel, Petit, Antoine, Gerard, Adeline, Marret, Emmanuel, Solier, Marc, Jaber, Samir, Prades, Albert, Krassler, Jens, Merzky, Simone, de Abreu, Marcel Gama, Uhlig, Christopher, Kiss, Thomas, Bundy, Anette, Bluth, Thomas, Gueldner, Andreas, Spieth, Peter, Scharffenberg, Martin, Thiem, Denny Tran, Koch, Thea, Treschan, Tanja, Schaefer, Maximilian, Bastin, Bea, Geib, Johann, Weiss, Martin, Kienbaum, Peter, Pannen, Benedikt, Gottschalk, Andre, Konrad, Mirja, Westerheide, Diana, Schwerdtfeger, Ben, Wrigge, Hermann, Simon, Philipp, Reske, Andreas, Nestler, Christian, Valsamidis, Dimitrios, Stroumpoulis, Konstantinos, Antholopoulos, Georgios, Andreou, Antonis, Karapanos, Dimitris, Theodoraki, Kassiani, Gkiokas, Georgios, Tasoulis, Marios-Konstantinos, Sidiropoulou, Tatiana, Zafeiropoulou, Foteini, Florou, Panagiota, Pandazi, Aggeliki, Tsaousi, Georgia, Nouris, Christos, Pourzitaki, Chryssa, Bystritski, Dmitri, Pizov, Reuven, Eden, Arieh, Pesce, Caterina Valeria, Campanile, Annamaria, Marrella, Antonella, Grasso, Salvatore, De Michele, Michele, Bona, Francesco, Giacoletto, Gianmarco, Sardo, Elena, Sottosanti, Luigi Giancarlo Vicari, Solca, Maurizio, Volta, Carlo Alberto, Spadaro, Savino, Verri, Marco, Ragazzi, Riccardo, Zoppellari, Roberto, Cinnella, Gilda, Raimondo, Pasquale, La Bella, Daniela, Mirabella, Lucia, D'antini, Davide, Pelosi, Paolo, Molin, Alexandre, Brunetti, Iole, Gratarola, Angelo, Pellerano, Giulia, Sileo, Rosanna, Pezzatto, Stefano, Montagnani, Luca, Pasin, Laura, Landoni, Giovanni, Zangrillo, Alberto, Beretta, Luigi, Di Parma, Ambra Licia, Tarzia, Valentina, Dossi, Roberto, Sassone, Marta Eugenia, Sances, Daniele, Tredici, Stefano, Spano, Gianluca, Castellani, Gianluca, Delunas, Luigi, Peradze, Sopio, Venturino, Marco, Arpino, Ines, Sher, Sara, Tommasino, Concezione, Rapido, Francesca, Morelli, Paola, Vargas, Maria, Servillo, Giuseppe, Cortegiani, Andrea, Raineri, Santi Maurizio, Montalto, Francesca, Russotto, Vincenzo, Giarratano, Antonino, Baciarello, Marco, Generali, Michela, Cerati, Giorgia, Leykin, Yigal, Bressan, Filippo, Bartolini, Vittoria, Zamidei, Lucia, Brazzi, Luca, Liperi, Corrado, Sales, Gabriele, Pistidda, Laura, Severgnini, Paolo, Brugnoni, Elisa, Musella, Giuseppe, Bacuzzi, Alessandro, Muhardri, Dalip, Gecaj-Gashi, Agreta, Sada, Fatos, Bytyqi, Adem, Karbonskiene, Aurika, Aukstakalniene, Ruta, Teberaite, Zivile, Salciute, Erika, Tikuisis, Renatas, Miliauskas, Povilas, Jurate, Sipylaite, Kontrimaviciute, Egle, Tomkute, Gabija, Xuereb, John, Bezzina, Maureen, Borg, Francis Joseph, Hemmes, Sabrine, Schultz, Marcus, Hollmann, Markus, Wiersma, Irene, Binnekade, Jan, Bos, Lieuwe, Nijbroek, Sunny, Boer, Christa, Duvekot, Anne, ’t Veld, Bas in, Werger, Alice, Dennesen, Paul, Severijns, Charlotte, De Jong, Jasper, Hering, Jens, van Beek, Rienk, Ivars, Stefan, Jammer, Ib, Breidablik, Alena, Hodt, Katharina Skirstad, Fjellanger, Frode, Avalos, Manuel Vico, Mellin-Olsen, Jannicke, Andersson, Elisabeth, Shafi-Kabiri, Amir, Molina, Ruby, Wutai, Stanley, Morais, Erick, Tareco, Glória, Ferreira, Daniel, Amaral, Joana, de Lurdes Goncalves Castro, Maria, Cadilha, Susana, Appleton, Sofia, Parente, Suzana, Correia, Mariana, Martins, Diogo, Monteirosa, Angela, Ricardo, Ana, Rodrigues, Sara, Horhota, Lucian, Grintescu, Ioana Marina, Mirea, Liliana, Grintescu, Ioana Cristina, Corneci, Dan, Negoita, Silvius, Dutu, Madalina, Garotescu, Ioana Popescu, Filipescu, Daniela, Prodan, Alexandru Bogdan, Droc, Gabriela, Fota, Ruxandra, Popescu, Mihai, Tomescu, Dana, Petcu, Ana Maria, Tudoroiu, Marian Irinel, Moise, Alida, Guran, Catalin-Traian, Gherghina, Iorel, Costea, Dan, Cindea, Iulia, Copotoiu, Sanda-Maria, Copotoiu, Ruxandra, Barsan, Victoria, Tolcser, Zsolt, Riciu, Magda, Moldovan, Septimiu Gheorghe, Veres, Mihaly, Gritsan, Alexey, Kapkan, Tatyana, Gritsan, Galina, Korolkov, Oleg, Kulikov, Alexander, Lubnin, Andrey, Ovezov, Alexey, Prokoshev, Pavel, Lugovoy, Alexander, Anipchenko, Natalia, Babayants, Andrey, Komissarova, Irina, Zalina, Karginova, Likhvantsev, Valery, Fedorov, Sergei, Lazukic, Aleksandra, Pejakovic, Jasmina, Mihajlovic, Dunja, Kusnierikova, Zuzana, Zelinkova, Maria, Bruncakova, Katarina, Polakovicova, Lenka, Sobona, Villiam, Novak-Supe, Barbka, Pekle-Golez, Ana, Jovanov, Miroljub, Strazisar, Branka, Markovic-Bozic, Jasmina, Novak-Jankovic, Vesna, Voje, Minca, Grynyuk, Andriy, Kostadinov, Ivan, Spindler-Vesel, Alenka, Moral, Victoria, Unzueta, Mari Carmen, Puigbo, Carlos, Fava, Josep, Canet, Jaume, Moret, Enrique, Nunez, Mónica Rodriguez, Sendra, Mar, Brunelli, Andrea, Rodenas, Frederic, Monedero, Pablo, Martinezv, Francisco Hidalgo, Temino, Maria Jose Yepes, Simon, Antonio Martínez, de Abajo Larriba, Ana, Lisi, Alberto, Perez, Gisela, Martinez, Raquel, Granell, Manuel, Vivo, Jose Tatay, Ruiz, Cristina Saiz, de Andrés Ibañez, Jose Antonio, Pastor, Ernesto, Soro, Marina, Ferrando, Carlos, Defez, Mario, Alvares-Santullano, Cesar Aldecoa, Perez, Rocio, Rico, Jesus, Jawad, Monir, Saeed, Yousif, Gillberg, Lars, Hedenstierma, Göran, Bengisun, Zuleyha Kazak, Kazbek, Baturay Kansu, Coskunfirat, Nesil, Boztug, Neval, Sanli, Suat, Yilmaz, Murat, Hadimioglu, Necmiye, Senturk, Nuzhet Mert, Camci, Emre, Kucukgoncu, Semra, Sungur, Zerrin, Sivrikoz, Nukhet, Ozgen, Serpil Ustalar, Toraman, Fevzi, Selvi, Onur, Senturk, Ozgur, Yildiz, Mine, Kuvaki, Bahar, Gunenc, Ferim, Kucukguclu, Semih, Ozbilgin, Şule, Maral, Jale, Canli, Seyda, Arun, Oguzhan, Saltali, Ali, Aydogan, Eyup, Akgun, Fatma Nur, Sanlikarip, Ceren, Karaman, Fatma Mine, Mazur, Andriy, Vorotyntsev, Sergiy, Rousseau, Guy, Barrett, Colin, Stancombe, Lucia, Shelley, Ben, Scholes, Helen, Limb, James, Rafi, Amir, Wayman, Lisa, Deane, Jill, Rogerson, David, Williams, John, Yates, Susan, Rogers, Elaine, Pulletz, Mark, Moreton, Sarah, Jones, Stephanie, Venkatesh, Suresh, Burton, Maudrian, Brown, Lucy, Goodall, Cait, Rucklidge, Matthew, Fuller, Debbie, Nadolski, Maria, Kusre, Sandeep, Lundberg, Michael, Everett, Lynn, Nutt, Helen, Zuleika, Maka, Carvalho, Peter, Clements, Deborah, Creagh-Brown, Ben, Watt, Philip, Raymode, Parizade, Pearse, Rupert, Mohr, Otto, Raj, Ashok, Creary, Thais, Chishti, Ahmed, Bell, Andrea, Higham, Charley, Cain, Alistair, Gibb, Sarah, Mowat, Stephen, Franklin, Danielle, West, Claire, Minto, Gary, Boyd, Nicholas, Mills, Gary, Calton, Emily, Walker, Rachel, Mackenzie, Felicity, Ellison, Branwen, Roberts, Helen, Chikungwa, Moses, Jackson, Clare, Donovan, Andrew, Foot, Jayne, Homan, Elizabeth, Montgomery, Jane, Portch, David, Mercer, Pauline, Palmer, Janet, Paddle, Jonathan, Fouracres, Anna, Datson, Amanda, Andrew, Alyson, Welch, Leanne, Rose, Alastair, Varma, Sandeep, Simeson, Karen, Rambhatla, Mrutyunjaya, Susarla, Jaysimha, Marri, Sudhakar, Kodaganallur, Krishnan, Das, Ashok, Algarsamy, Shivarajan, Colley, Julie, Davies, Simon, Szewczyk, Margaret, Smith, Thomas, Fernandez-Bustamante, Ana, Luzier, Elizabeth, Almagro, Angela, Melo, Marcos Vidal, Fernando, Luiz, Sulemanji, Demet, Sprung, Juraj, Weingarten, Toby, Kor, Daryl, Scavonetto, Federica, Tze, Yeo, Vermeulen, Tom D., Hol, Liselotte, Swart, Pien, Mills, Gary H., Putensen, Christian, Serpa Neto, Ary, Vidal Melo, Marcos F., Hollmann, Markus W., Gama de Abreu, Marcelo, Schultz, Marcus J., Hemmes, Sabrine N., and van Meenen, David M.

Published
2024
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20. Associations of intraoperative end–tidal CO2 levels with postoperative outcome–secondary analysis of a worldwide observational study

Author

Kroell, Wolfgang, Metzler, Helfried, Struber, Gerd, Wegscheider, Thomas, Gombotz, Hans, Hiesmayr, Michael, Schmid, Werner, Urbanek, Bernhard, Kahn, David, Momeni, Mona, Pospiech, Audrey, Lois, Fernande, Forget, Patrice, Grosu, Irina, Poelaert, Jan, van Mossevelde, Veerle, van Malderen, Marie-Claire, Dylst, Dimitri, van Melkebeek, Jeroen, Beran, Maud, de Hert, Stefan, De Baerdemaeker, Luc, Heyse, Bjorn, Van Limmen, Jurgen, Wyffels, Piet, Jacobs, Tom, Roels, Nathalie, De Bruyne, Ann, van de Velde, Stijn, Leva, Brigitte, Damster, Sandrine, Plichon, Benoit, JurosZovko, Marina, Djonovic-Omanoviċ, Dejana, Pernar, Selma, Zunic, Josip, Miskovic, Petar, Zilic, Antonio, Kvolik, Slavica, Ivic, Dubravka, Azenic-Venzera, Darija, Skiljic, Sonja, Vinkovic, Hrvoje, Oputric, Ivana, Juricic, Kazimir, Frkovic, Vedran, Kopic, Jasminka, Mirkovic, Ivan, Karanovic, Nenad, Carev, Mladen, Dropulic, Natasa, Saric, Jadranka Pavicic, Erceg, Gorjana, Dvorscak, Matea Bogdanovic, Mazul-Sunko, Branka, Pavicic, Anna Marija, Goranovic, Tanja, Maldini, Branka, Radocaj, Tomislav, Gavranovic, Zeljka, Mladic-Batinica, Inga, Sehovic, Mirna, Stourac, Petr, Harazim, Hana, Smekalova, Olga, Kosinova, Martina, Kolacek, Tomas, Hudacek, Kamil, Drab, Michal, Brujevic, Jan, Vitkova, Katerina, Jirmanova, Katerina, Volfova, Ivana, Dzurnakova, Paula, Liskova, Katarina, Dudas, Radovan, Filipsky, Radek, El Kafrawy, Samir, Abdelwahab, Hisham Hosny, Metwally, Tarek, Abdel-Razek, Ahmed, El-Shaarawy, Ahmed Mostafa, Hasan, Wael Fathy, Ahmed, Ahmed Gouda, Yassin, Hany, Magdy, Mohamed, Abdelhady, Mahdy, Mahran, Mohamed, Herodes, Eiko, Kivik, Peeter, Oganjan, Juri, Aun, Annika, Sormus, Alar, Sarapuu, Kaili, Mall, Merilin, Karjagin, Juri, Futier, Emmanuel, Petit, Antoine, Gerard, Adeline, Marret, Emmanuel, Solier, Marc, Jaber, Samir, Prades, Albert, Krassler, Jens, Merzky, Simone, de Abreu, Marcel Gama, Uhlig, Christopher, Kiss, Thomas, Bundy, Anette, Bluth, Thomas, Gueldner, Andreas, Spieth, Peter, Scharffenberg, Martin, Thiem, Denny Tran, Koch, Thea, Treschan, Tanja, Schaefer, Maximilian, Bastin, Bea, Geib, Johann, Weiss, Martin, Kienbaum, Peter, Pannen, Benedikt, Gottschalk, Andre, Konrad, Mirja, Westerheide, Diana, Schwerdtfeger, Ben, Wrigge, Hermann, Simon, Philipp, Reske, Andreas, Nestler, Christian, Valsamidis, Dimitrios, Stroumpoulis, Konstantinos, Antholopoulos, Georgios, Andreou, Antonis, Karapanos, Dimitris, Theodoraki, Kassiani, Gkiokas, Georgios, Tasoulis, Marios-Konstantinos, Sidiropoulou, Tatiana, Zafeiropoulou, Foteini, Florou, Panagiota, Pandazi, Aggeliki, Tsaousi, Georgia, Nouris, Christos, Pourzitaki, Chryssa, Bystritski, Dmitri, Pizov, Reuven, Eden, Arieh, Pesce, Caterina Valeria, Campanile, Annamaria, Marrella, Antonella, Grasso, Salvatore, De Michele, Michele, Bona, Francesco, Giacoletto, Gianmarco, Sardo, Elena, Giancarlo, Luigi, Sottosanti, Vicari, Solca, Maurizio, Volta, Carlo Alberto, Spadaro, Savino, Verri, Marco, Ragazzi, Riccardo, Zoppellari, Roberto, Cinnella, Gilda, Raimondo, Pasquale, La Bella, Daniela, Mirabella, Lucia, D’antini, Davide, Pelosi, Paolo, Molin, Alexandre, Brunetti, Iole, Gratarola, Angelo, Pellerano, Giulia, Sileo, Rosanna, Pezzatto, Stefano, Montagnani, Luca, Pasin, Laura, Landoni, Giovanni, Zangrillo, Alberto, Beretta, Luigi, Di Parma, Ambra Licia, Tarzia, Valentina, Dossi, Roberto, Sassone, Marta Eugenia, Sances, Daniele, Tredici, Stefano, Spano, Gianluca, Castellani, Gianluca, Delunas, Luigi, Peradze, Sopio, Venturino, Marco, Arpino, Ines, Sher, Sara, Tommasino, Concezione, Rapido, Francesca, Morelli, Paola, Vargas, Maria, Servillo, Giuseppe, Cortegiani, Andrea, Raineri, Santi Maurizio, Montalto, Francesca, Russotto, Vincenzo, Giarratano, Antonino, Baciarello, Marco, Generali, Michela, Cerati, Giorgia, Leykin, Yigal, Bressan, Filippo, Bartolini, Vittoria, Zamidei, Lucia, Brazzi, Luca, Liperi, Corrado, Sales, Gabriele, Pistidda, Laura, Severgnini, Paolo, Brugnoni, Elisa, Musella, Giuseppe, Bacuzzi, Alessandro, Muhardri, Dalip, Gecaj-Gashi, Agreta, Sada, Fatos, Bytyqi, Adem, Karbonskiene, Aurika, Aukstakalniene, Ruta, Teberaite, Zivile, Salciute, Erika, Tikuisis, Renatas, Miliauskas, Povilas, Jurate, Sipylaite, Kontrimaviciute, Egle, Tomkute, Gabija, Xuereb, John, Bezzina, Maureen, Borg, Francis Joseph, Hemmes, Sabrine, Schultz, Marcus, Hollmann, Markus, Wiersma, Irene, Binnekade, Jan, Bos, Lieuwe, Boer, Christa, Duvekot, Anne, In’t Veld, Bas, Werger, Alice, Dennesen, Paul, Severijns, Charlotte, De Jong, Jasper, Hering, Jens, van Beek, Rienk, Ivars, Stefan, Jammer, Ib, Breidablik, Alena, Hodt, Katharina Skirstad, Fjellanger, Frode, Avalos, Manuel Vico, MellinOlsen, Jannicke, Andersson, Elisabeth, Shafi-Kabiri, Amir, Molina, Ruby, Wutai, Stanley, Morais, Erick, Tareco, Gloria, Ferreira, Daniel, Amaral, Joana, de Lurdes Goncalves Castro, Maria, Cadilha, Susana, Appleton, Sofia, Parente, Suzana, Correia, Mariana, Martins, Diogo, Monteirosa, Angela, Ricardo, Ana, Rodrigues, Sara, Horhota, Lucian, Grintescu, Ioana Marina, Mirea, Liliana, Grintescu, Ioana Cristina, Corneci, Dan, Negoita, Silvius, Dutu, Madalina, Garotescu, Ioana Popescu, Filipescu, Daniela, Prodan, Alexandru Bogdan, Droc, Gabriela, Fota, Ruxandra, Popescu, Mihai, Tomescu, Dana, Petcu, Ana Maria, Tudoroiu, Marian Irinel, Moise, Alida, Guran, Catalin-Traian, Gherghina, Iorel, Costea, Dan, Cindea, Iulia, Copotoiu, Sanda-Maria, Copotoiu, Ruxandra, Barsan, Victoria, Tolcser, Zsolt, Riciu, Magda, Moldovan, Septimiu Gheorghe, Veres, Mihaly, Gritsan, Alexey, Kapkan, Tatyana, Gritsan, Galina, Korolkov, Oleg, Kulikov, Alexander, Lubnin, Andrey, Ovezov, Alexey, Prokoshev, Pavel, Lugovoy, Alexander, Anipchenko, Natalia, Babayants, Andrey, Komissarova, Irina, Zalina, Karginova, Likhvantsev, Valery, Fedorov, Sergei, Lazukic, Aleksandra, Pejakovic, Jasmina, Mihajlovic, Dunja, Kusnierikova, Zuzana, Zelinkova, Maria, Bruncakova, Katarina, Polakovicova, Lenka, Sobona, Villiam, Novak-Supe, Barbka, Pekle-Golez, Ana, Jovanov, Miroljub, Strazisar, Branka, Markovic-Bozic, Jasmina, Novak-Jankovic, Vesna, Voje, Minca, Grynyuk, Andriy, Kostadinov, Ivan, Spindler-Vesel, Alenka, Moral, Victoria, Unzueta, Mari Carmen, Puigbo, Carlos, Fava, Josep, Canet, Jaume, Moret, Enrique, Nunez, Monicá Rodriguez, Sendra, Mar, Brunelli, Andrea, Rodenas, Frederic, Monedero, Pablo, Martinez, Francisco Hidalgo, Temino, Maria Jose Yepes, Simon, Antonio Martínez, de Abajo Larriba, Ana, Lisi, Alberto, Perez, Gisela, Martinez, Raquel, Granell, Manuel, Vivo, Jose Tatay, Ruiz, Cristina Saiz, de Andrés Ibañez, Jose Antonio, Pastor, Ernesto, Soro, Marina, Ferrando, Carlos, Defez, Mario, Alvares-Santullano, Cesar Aldecoa, Perez, Rocio, Rico, Jesus, Jawad, Monir, Saeed, Yousif, Gillberg, Lars, Bengisun, Zuleyha Kazak, Kazbek, Baturay Kansu, Coskunfirat, Nesil, Boztug, Neval, Sanli, Suat, Yilmaz, Murat, Hadimioglu, Necmiye, Senturk, Nuzhet Mert, Camci, Emre, Kucukgoncu, Semra, Sungur, Zerrin, Sivrikoz, Nukhet, Ozgen, Serpil Ustalar, Toraman, Fevzi, Selvi, Onur, Senturk, Ozgur, Yildiz, Mine, Kuvaki, Bahar, Gunenc, Ferim, Kucukguclu, Semih, Ozbilgin, Şule, Maral, Jale, Canli, Seyda, Arun, Oguzhan, Saltali, Ali, Aydogan, Eyup, Akgun, Fatma Nur, Sanlikarip, Ceren, Karaman, Fatma Mine, Mazur, Andriy, Vorotyntsev, Sergiy, Rousseau, Guy, Barrett, Colin, Stancombe, Lucia, Shelley, Ben, Scholes, Helen, Limb, James, Rafi, Amir, Wayman, Lisa, Deane, Jill, Rogerson, David, Williams, John, Yates, Susan, Rogers, Elaine, Pulletz, Mark, Moreton, Sarah, Jones, Stephanie, Venkatesh, Suresh, Burton, Maudrian, Brown, Lucy, Goodall, Cait, Rucklidge, Matthew, Fuller, Debbie, Nadolski, Maria, Kusre, Sandeep, Lundberg, Michael, Everett, Lynn, Nutt, Helen, Zuleika, Maka, Carvalho, Peter, Clements, Deborah, Creagh-Brown, Ben, Watt, Philip, Raymode, Parizade, Pearse, Rupert, Mohr, Otto, Raj, Ashok, Creary, Thais, Chishti, Ahmed, Bell, Andrea, Higham, Charley, Cain, Alistair, Gibb, Sarah, Mowat, Stephen, Franklin, Danielle, West, Claire, Minto, Gary, Boyd, Nicholas, Mills, Gary, Calton, Emily, Walker, Rachel, Mackenzie, Felicity, Ellison, Branwen, Roberts, Helen, Chikungwa, Moses, Jackson, Clare, Donovan, Andrew, Foot, Jayne, Homan, Elizabeth, Montgomery, Jane, Portch, David, Mercer, Pauline, Palmer, Janet, Paddle, Jonathan, Fouracres, Anna, Datson, Amanda, Andrew, Alyson, Welch, Leanne, Rose, Alastair, Varma, Sandeep, Simeson, Karen, Rambhatla, Mrutyunjaya, Susarla, Jaysimha, Marri, Sudhakar, Kodaganallur, Krishnan, Das, Ashok, Algarsamy, Shivarajan, Colley, Julie, Davies, Simon, Szewczyk, Margaret, Smith, Thomas, Fernandez-Bustamante, Ana, Luzier, Elizabeth, Almagro, Angela, Melo, Marcos Vidal, Fernando, Luiz, Sulemanji, Demet, Sprung, Juraj, Weingarten, Toby, Kor, Daryl, Scavonetto, Federica, Tze, Yeo, Nasa, Prashant, van Meenen, David M.P., Paulus, Frederique, de Abreu, Marcelo Gama, Bossers, Sebastiaan M., Schober, Patrick, Schultz, Marcus J., Neto, Ary Serpa, and Hemmes, Sabrine N.T.

Published
2025
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21. Development of Indirect Sandwich ELLA for Detection of Insects in Food

Author

Matej Pospiech, Martina Pečová, Marie Bartlová, Zdeňka Javůrková, Anežka Kopecká, Kateřina Šebelová, Ondřej Pospíšil, Martin Kulma, Jakub Folke, Bohuslava Tremlová, Lenka Kouřimská, and Jana Hajšlová

Subjects
N-acetylglucosamine, chitin, chitosan, food adulteration, edible insect, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
Abstract

Edible insects have been officially classified as food in the European Union since 2015. Currently, four insect species are approved for food use. However, no official method for detecting insects in food has been recognized to date. To establish a simple analytical method for insect detection in food, we developed an indirect sandwich (enzyme-linked lectin sorbent assay) ELLA specific for N-acetylglucosamine in chitin and chitosan polymers. The validation of the method demonstrated that the ELLA developed in this study is reliable for insect detection. The limit of detection (LOD) and quantification (LOQ) were 0.006 and 0.028 mg/mL, respectively. Intra-day precision ranged from 2.45% to 30.29%, and inter-day precision from 0.36% to 12.87%. Significant differences in the total amount of chitin and chitosan were observed among the insect products, processed insect products, and samples without any insect addition (p < 0.05).

Published
2024
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26. Impact of Air Jet Impingement Technology on the Strength of Tissue Paper

Author

Mariusz Reczulski, Piotr Pospiech, Kinga Troszczyńska, and Maria Bieńkowska

Subjects
tissue paper, impingement air jets, tensile strength of paper, Biotechnology, TP248.13-248.65
Abstract

Impinging air jets can be used to dewater, heat, and dry the web of tissue paper. High velocities of the air jets degrade the paper, and appropriate adjustments to the jet velocity and the distance of the nozzle from the surface of the wet web are crucial to obtain the highest quality product. This work investigated the correlation between the velocity of the air jet and the strength of paper subjected to the impingement method. Papers with an initial moisture content of 20% and various pulp mixes were tested, and the physical properties of papers were explored. After impinging an air jet, different tensile strength limits were obtained in the machine and cross directions. The paper had lower apparent density and higher roughness compared to classical pressing. The dependence of tensile strength and roughness on the fibers composition also was determined. Increasing the amount of eucalyptus fibers in impingement dewatered paper resulted in a decrease in its tensile strength and roughness. The value of elongation before breaking was the highest for softwood papers after the impingement method. The maximum velocity of an air jet that can be used to dewater or dry paper without the risk of damage to the papers was determined.

Published
2024

27. MCPIP1 Inhibits Hepatic Stellate Cell Activation in Autocrine and Paracrine Manners, Preventing Liver FibrosisSummary

Author

Natalia Pydyn, Anna Ferenc, Katarzyna Trzos, Ewelina Pospiech, Mateusz Wilamowski, Olga Mucha, Piotr Major, Justyna Kadluczka, Pedro M. Rodrigues, Jesus M. Banales, Jose M. Herranz, Matias A. Avila, Tomasz Hutsch, Piotr Malczak, Dorota Radkowiak, Andrzej Budzynski, Jolanta Jura, and Jerzy Kotlinowski

Subjects
MCPIP1, Liver, Fibrosis, HSCs, Inflammation, Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

Background & Aims: Hepatic fibrosis is characterized by enhanced deposition of extracellular matrix (ECM), which results from the wound healing response to chronic, repeated injury of any etiology. Upon injury, hepatic stellate cells (HSCs) activate and secrete ECM proteins, forming scar tissue, which leads to liver dysfunction. Monocyte-chemoattractant protein-induced protein 1 (MCPIP1) possesses anti-inflammatory activity, and its overexpression reduces liver injury in septic mice. In addition, mice with liver-specific deletion of Zc3h12a develop features of primary biliary cholangitis. In this study, we investigated the role of MCPIP1 in liver fibrosis and HSC activation. Methods: We analyzed MCPIP1 levels in patients’ fibrotic livers and hepatic cells isolated from fibrotic murine livers. In vitro experiments were conducted on primary HSCs, cholangiocytes, hepatocytes, and LX-2 cells with MCPIP1 overexpression or silencing. Results: MCPIP1 levels are induced in patients’ fibrotic livers compared with their nonfibrotic counterparts. Murine models of fibrosis revealed that its level is increased in HSCs and hepatocytes. Moreover, hepatocytes with Mcpip1 deletion trigger HSC activation via the release of connective tissue growth factor. Overexpression of MCPIP1 in LX-2 cells inhibits their activation through the regulation of TGFB1 expression, and this phenotype is reversed upon MCPIP1 silencing. Conclusions: We demonstrated that MCPIP1 is induced in human fibrotic livers and regulates the activation of HSCs in both autocrine and paracrine manners. Our results indicate that MCPIP1 could have a potential role in the development of liver fibrosis.

Published
2024
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28. Influencing ionic conductivity and mechanical properties of ionic liquid polymer electrolytes by designing the chemical monomer structure

Author

Lisa Ehrlich, Doris Pospiech, Petra Uhlmann, Felix Tzschöckell, Martin D. Hager, and Brigitte Voit

Subjects
lonic liquids, chloride-conducting polymers, polymer electrolytes, polymer electrolyte networks, organic radical batteries, Polymers and polymer manufacture, TP1080-1185
Abstract

Polymeric single chloride-ion conductor networks based on acrylic imidazolium chloride ionic liquid monomers AACXImCYCl as reported previously are prepared. The chemical structure of the polymers is varied with respect to the acrylic substituents (alkyl spacer and alkyl substituent in the imidazolium ring). The networks are examined in detail with respect to the influence of the chemical structure on the resulting properties including thermal behavior, rheological behavior, swelling behavior, and ionic conductivity. The ionic conductivities increase (by two orders of magnitude from 10−6 to 10−4 S·cm−1 with increasing temperature), while the complex viscosities of the polymer networks decrease simultaneously. After swelling in water for 1 week the ionic conductivity reaches values of 10−2 S·cm−1. A clear influence of the spacer and the crosslinker content on the glass transition temperature was shown for the first time in these investigations. With increasing crosslinker content, the Tg values and the viscosities of the networks increase. With increasing spacer length, the Tg values decrease, but the viscosities increase with increasing temperature. The results reveal that the materials represent promising electrolytes for batteries, as proven by successful charging/discharging of a p(TEMPO-MA)/zinc battery over 350 cycles.

Published
2023
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30. Nuclear structure investigations of Es253−255 by laser spectroscopy

Author

Nothhelfer, S, Albrecht-Schönzart, Th E, Block, M, Chhetri, P, Düllmann, Ch E, Ezold, JG, Gadelshin, V, Gaiser, A, Giacoppo, F, Heinke, R, Kieck, T, Kneip, N, Laatiaoui, M, Mokry, Ch, Raeder, S, Runke, J, Schneider, F, Sperling, JM, Studer, D, Thörle-Pospiech, P, Trautmann, N, Weber, F, and Wendt, K

Subjects
Atomic, Molecular, Nuclear, Particle and Plasma Physics, Nuclear & Particles Physics
Abstract

Laser resonance ionization spectroscopy was performed on the rare einsteinium isotopes Es253-255 at the RISIKO mass separator in Mainz. With low sample sizes ranging down to femtograms, the prominent 352 nm-ground-state transition was measured in all three einsteinium isotopes, and four additional ground-state transitions were measured in Es254. Hyperfine-structure analysis resulted in assigned spin values of I(Es254)=7 and I(Es255)=7/2. From the extracted coupling constants, nuclear magnetic dipole moments of μI(Es254)=3.42(7)μN and μI(Es255)=4.14(10)μN as well as spectroscopic electric quadrupole moments of Qs(Es254)=9.6(1.2)eb and Qs(Es255)=5.1(1.7)eb were derived. Our value for Es254 deviates from the value of |μI(Es254)|=4.35(41)μN extracted from the angular anisotropy of α-radiation emitted by Es254.

Published
2022

31. Design and evaluation of a questionnaire to assess learners’ understanding of quantum measurement in different two-state contexts: The context matters

Author

Philipp Bitzenbauer, Sergej Faletič, Marisa Michelini, Kristóf Tóth, and Gesche Pospiech

Subjects
Special aspects of education, LC8-6691, Physics, QC1-999
Abstract

The teaching and learning of quantum physics has recently become a topic of increasing interest in physics education research. In particular, the study of two-state systems is gaining importance as a means of teaching quantum physics at various educational levels. Meanwhile, a number of approaches have been developed that are also suitable for high school students. It can be assumed that the different approaches have different degrees of effectiveness in teaching central quantum concepts. However, suitable evaluation instruments to test this are still lacking. Therefore, as a first step, a short questionnaire on quantum measurement, suitable for both research and classroom use, was developed in several steps. First, a questionnaire with open and closed items was created and piloted with a total of N=120 learners. The responses were evaluated qualitatively using a comprehensive coding manual, which provided insights into learners’ conceptions. These results led to the development of an eight-item questionnaire that could be adapted to different teaching approaches. This questionnaire was subjected to expert review and, finally, successfully tested for its psychometric properties with a sample of N=201 learners. Overall, our results provide initial empirical evidence that context (i.e., which two-state approach is used) does matter for student learning, but in general, two-state approaches appear to be particularly conducive to learning quantum concepts (specified in this article for quantum measurement) compared to traditional instruction.

Published
2024
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32. High-dose chemotherapy and autologous haematopoietic stem-cell transplantation in older, fit patients with primary diffuse large B-cell CNS lymphoma (MARTA): a single-arm, phase 2 trial

Author

Schorb, Elisabeth, Isbell, Lisa Kristina, Kerkhoff, Andrea, Mathas, Stephan, Braulke, Friederike, Egerer, Gerlinde, Röth, Alexander, Schliffke, Simon, Borchmann, Peter, Brunnberg, Uta, Kroschinsky, Frank, Möhle, Robert, Rank, Andreas, Wellnitz, Dominique, Kasenda, Benjamin, Pospiech, Lisa, Wendler, Julia, Scherer, Florian, Deckert, Martina, Henkes, Elina, von Gottberg, Philipp, Gmehlin, Dennis, Backenstraß, Matthias, Jensch, Antje, Burger-Martin, Elvira, Grishina, Olga, Fricker, Heidi, Malenica, Natalie, Orbán, András, Duyster, Justus, Ihorst, Gabriele, Finke, Juergen, and Illerhaus, Gerald

Published
2024
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34. An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Author

Schoof, Michael, Faust, Bryan, Saunders, Reuben A, Sangwan, Smriti, Rezelj, Veronica, Hoppe, Nick, Boone, Morgane, Billesbølle, Christian B, Puchades, Cristina, Azumaya, Caleigh M, Kratochvil, Huong T, Zimanyi, Marcell, Deshpande, Ishan, Liang, Jiahao, Dickinson, Sasha, Nguyen, Henry C, Chio, Cynthia M, Merz, Gregory E, Thompson, Michael C, Diwanji, Devan, Schaefer, Kaitlin, Anand, Aditya A, Dobzinski, Niv, Zha, Beth Shoshana, Simoneau, Camille R, Leon, Kristoffer, White, Kris M, Chio, Un Seng, Gupta, Meghna, Jin, Mingliang, Li, Fei, Liu, Yanxin, Zhang, Kaihua, Bulkley, David, Sun, Ming, Smith, Amber M, Rizo, Alexandrea N, Moss, Frank, Brilot, Axel F, Pourmal, Sergei, Trenker, Raphael, Pospiech, Thomas, Gupta, Sayan, Barsi-Rhyne, Benjamin, Belyy, Vladislav, Barile-Hill, Andrew W, Nock, Silke, Liu, Yuwei, Krogan, Nevan J, Ralston, Corie Y, Swaney, Danielle L, García-Sastre, Adolfo, Ott, Melanie, Vignuzzi, Marco, Walter, Peter, Manglik, Aashish, Braxton, Julian R, Lopez, Kyle E, Melo, Arthur, Paulino, Joana, Pospiech, Thomas H, Thomas, Paul V, Wang, Feng, Yu, Zanlin, Dickinson, Miles Sasha, Asarnow, Daniel, Campbell, Melody G, Li, Junrui, Tsui, Tsz Kin Martin, Trinidad, Donovan, Tse, Eric, Zhou, Fengbo, Herrera, Nadia, and Schulze-Gahmen, Ursula

Subjects
Biochemistry and Cell Biology, Biological Sciences, Infectious Diseases, Emerging Infectious Diseases, Coronaviruses, Biodefense, Infection, Angiotensin-Converting Enzyme 2, Animals, Antibodies, Neutralizing, Antibodies, Viral, Antibody Affinity, Chlorocebus aethiops, Cryoelectron Microscopy, Humans, Neutralization Tests, Protein Binding, Protein Stability, Single-Domain Antibodies, Spike Glycoprotein, Coronavirus, Vero Cells, QCRG Structural Biology Consortium, General Science & Technology
Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus enters host cells via an interaction between its Spike protein and the host cell receptor angiotensin-converting enzyme 2 (ACE2). By screening a yeast surface-displayed library of synthetic nanobody sequences, we developed nanobodies that disrupt the interaction between Spike and ACE2. Cryo-electron microscopy (cryo-EM) revealed that one nanobody, Nb6, binds Spike in a fully inactive conformation with its receptor binding domains locked into their inaccessible down state, incapable of binding ACE2. Affinity maturation and structure-guided design of multivalency yielded a trivalent nanobody, mNb6-tri, with femtomolar affinity for Spike and picomolar neutralization of SARS-CoV-2 infection. mNb6-tri retains function after aerosolization, lyophilization, and heat treatment, which enables aerosol-mediated delivery of this potent neutralizer directly to the airway epithelia.

Published
2020

38. Patients With Immunoglobulin A Nephropathy Show Abnormal Frequencies of B Cell Subsets, Unconventional T Cells, and High Levels of Galactose-Deficient IgA1–Coated Gut Bacteria

Author

Gentile, Micaela, Goerlich, Nina, Lo, I-Ju, Olson, N. Eric, McConnell, Mark, Pospiech, Johannes, Bohnenpoll, Tobias, Skroblin, Philipp, Radresa, Olivier, Andag, Uwe, Campbell, Kirk N., Meliambro, Kristin, Sanchez-Russo, Luis, Verlato, Alberto, Fiaccadori, Enrico, Kim-Schulze, Seunghee, Lanau, Maria, Fernandez-Lorente, M. Loreto, Fribourg, Miguel, Manrique, Joaquin, and Cravedi, Paolo

Published
2024
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43. CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes

Author

Gupta, Meghna, Azumaya, Caleigh M, Moritz, Michelle, Pourmal, Sergei, Diallo, Amy, Merz, Gregory E, Jang, Gwendolyn, Bouhaddou, Mehdi, Fossati, Andrea, Brilot, Axel F, Diwanji, Devan, Hernandez, Evelyn, Herrera, Nadia, Kratochvil, Huong T, Lam, Victor L, Li, Fei, Li, Yang, Nguyen, Henry C, Nowotny, Carlos, Owens, Tristan W, Peters, Jessica K, Rizo, Alexandrea N, Schulze-Gahmen, Ursula, Smith, Amber M, Young, Iris D, Yu, Zanlin, Asarnow, Daniel, Billesbølle, Christian, Campbell, Melody G, Chen, Jen, Chen, Kuei-Ho, Chio, Un Seng, Dickinson, Miles Sasha, Doan, Loan, Jin, Mingliang, Kim, Kate, Li, Junrui, Li, Yen-Li, Linossi, Edmond, Liu, Yanxin, Lo, Megan, Lopez, Jocelyne, Lopez, Kyle E, Mancino, Adamo, Moss, Frank R, Paul, Michael D, Pawar, Komal Ishwar, Pelin, Adrian, Pospiech, Thomas H, Puchades, Cristina, Remesh, Soumya Govinda, Safari, Maliheh, Schaefer, Kaitlin, Sun, Ming, Tabios, Mariano C, Thwin, Aye C, Titus, Erron W, Trenker, Raphael, Tse, Eric, Tsui, Tsz Kin Martin, Wang, Feng, Zhang, Kaihua, Zhang, Yang, Zhao, Jianhua, Zhou, Fengbo, Zhou, Yuan, Zuliani-Alvarez, Lorena, Consortium, QCRG Structural Biology, Agard, David A, Cheng, Yifan, Fraser, James S, Jura, Natalia, Kortemme, Tanja, Manglik, Aashish, Southworth, Daniel R, Stroud, Robert M, Swaney, Danielle L, Krogan, Nevan J, Frost, Adam, Rosenberg, Oren S, and Verba, Kliment A

Subjects
Biochemistry and Cell Biology, Bioinformatics and Computational Biology, Biological Sciences, Coronaviruses, Emerging Infectious Diseases, Genetics, Infectious Diseases, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, 2.2 Factors relating to the physical environment, Infection, Generic health relevance
Abstract

The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis of those functions, remain unknown. Here, we report an atomic model for full-length Nsp2 obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. The resulting structure reveals a highly-conserved zinc ion-binding site, suggesting a role for Nsp2 in RNA binding. Mapping emerging mutations from variants of SARS-CoV-2 on the resulting structure shows potential host-Nsp2 interaction regions. Using structural analysis together with affinity tagged purification mass spectrometry experiments, we identify Nsp2 mutants that are unable to interact with the actin-nucleation-promoting WASH protein complex or with GIGYF2, an inhibitor of translation initiation and modulator of ribosome-associated quality control. Our work suggests a potential role of Nsp2 in linking viral transcription within the viral replication-transcription complexes (RTC) to the translation initiation of the viral message. Collectively, the structure reported here, combined with mutant interaction mapping, provides a foundation for functional studies of this evolutionary conserved coronavirus protein and may assist future drug design.

Published
2021

44. Deletion of CD36 exhibits limited impact on normal hematopoiesis and the leukemia microenvironment

Author

Yiting Meng, Mateusz Pospiech, Atham Ali, Ritu Chandwani, Mary Vergel, Sandra Onyemaechi, George Yaghmour, Rong Lu, and Houda Alachkar

Subjects
CD36, Knockout, Hematopoiesis, T Cells, Acute myeloid leukemia, Mouse, Cytology, QH573-671
Abstract

Abstract Background CD36 has been identified as a potential therapeutic target both in leukemic cells and in the tumor immune microenvironment. In acute myeloid leukemia (AML), we found that APOC2 acts with CD36 to promote leukemia growth by activating the LYN-ERK signaling. CD36 also plays a role in lipid metabolism of cancer associated T-cells leading to impaired cytotoxic CD8+ T-cell and enhanced Treg cell function. To establish CD36 as a viable therapeutic target in AML, we investigated whether targeting CD36 has any detrimental impact on normal hematopoietic cells. Methods Differential expression data of CD36 during human and mouse normal hematopoiesis were examined and compared. Cd36 knockout (Cd36-KO) mice were evaluated for blood analysis, hematopoietic stem cells and progenitors (HSPCs) function and phenotype analyses, and T cells in vitro expansion and phenotypes in comparison with wild type (WT) mice. In addition, MLL-PTD/FLT3-ITD leukemic cells were engrafted into Cd36-KO and WT mice, and leukemia burden was compared between groups. Results RNA-Seq data showed that Cd36 expression was low in HSPCs and increased as cells matured. Phenotypic analysis revealed limited changes in blood count except for a slight yet significantly lower red blood cell count and hemoglobin and hematocrit levels in Cd36-KO mice compared with WT mice (P

Published
2023
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45. ABRAXAS1 orchestrates BRCA1 activities to counter genome destabilizing repair pathways—lessons from breast cancer patients

Author

Juliane Sachsenweger, Rebecca Jansche, Tatjana Merk, Benedikt Heitmeir, Miriam Deniz, Ulrike Faust, Cristiana Roggia, Andreas Tzschach, Christopher Schroeder, Angelika Riess, Helmut Pospiech, Hellevi Peltoketo, Katri Pylkäs, Robert Winqvist, and Lisa Wiesmüller

Subjects
Cytology, QH573-671
Abstract

Abstract It has been well-established that mutations in BRCA1 and BRCA2, compromising functions in DNA double-strand break repair (DSBR), confer hereditary breast and ovarian cancer risk. Importantly, mutations in these genes explain only a minor fraction of the hereditary risk and of the subset of DSBR deficient tumors. Our screening efforts identified two truncating germline mutations in the gene encoding the BRCA1 complex partner ABRAXAS1 in German early-onset breast cancer patients. To unravel the molecular mechanisms triggering carcinogenesis in these carriers of heterozygous mutations, we examined DSBR functions in patient-derived lymphoblastoid cells (LCLs) and in genetically manipulated mammary epithelial cells. By use of these strategies we were able to demonstrate that these truncating ABRAXAS1 mutations exerted dominant effects on BRCA1 functions. Interestingly, we did not observe haploinsufficiency regarding homologous recombination (HR) proficiency (reporter assay, RAD51-foci, PARP-inhibitor sensitivity) in mutation carriers. However, the balance was shifted to use of mutagenic DSBR-pathways. The dominant effect of truncated ABRAXAS1 devoid of the C-terminal BRCA1 binding site can be explained by retention of the N-terminal interaction sites for other BRCA1-A complex partners like RAP80. In this case BRCA1 was channeled from the BRCA1-A to the BRCA1-C complex, which induced single-strand annealing (SSA). Further truncation, additionally deleting the coiled-coil region of ABRAXAS1, unleashed excessive DNA damage responses (DDRs) de-repressing multiple DSBR-pathways including SSA and non-homologous end-joining (NHEJ). Our data reveal de-repression of low-fidelity repair activities as a common feature of cells from patients with heterozygous mutations in genes encoding BRCA1 and its complex partners.

Published
2023
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48. Exploring the Influence of Soil Types on the Mineral Profile of Honey: Implications for Geographical Origin Prediction

Author

Simona Schmidlová, Zdeňka Javůrková, Bohuslava Tremlová, Józef Hernik, Barbara Prus, Slavomír Marcinčák, Dana Marcinčáková, Pavel Štarha, Helena Čížková, Vojtěch Kružík, Zsanett Bodor, Csilla Benedek, Dalibor Titěra, Jana Boržíková, and Matej Pospiech

Subjects
traces elements, Czech beekeepers, sustainability, GIS, Chemical technology, TP1-1185
Abstract

Honey contains a wide range of inorganic substances. Their content can be influenced, i.e., by the type of soil on which the bee pasture is located. As part of this study, the mineral profile of 32 samples of honey from hobby beekeepers from the Czech Republic wasevaluated and then compared with soil types in the vicinity of the beehive location. Pearson’s correlation coefficient was used to express the relationship between mineral substances and soil type. There was a high correlation between antroposol and Zn (R = 0.98), Pb (R = 0.96), then between ranker and Mn (0.95), then regosol and Al (R = 0.97) (p < 0.05). A high negative correlation was found between regosol and Mg (R = −0.97), Cr (R = −0.98) and between redzinas and Al (R = −0.97) (p < 0.05). Both positive and negative high correlations were confirmed for phaeozem. The CART method subsequently proved that the characteristic elements for individual soil types are B, Ca, Mg, Ni, and Mn. The soil types of cambisol, fluvisol, gleysol, anthrosol, and kastanozem had the closest relationship with the elements mentioned, and it can therefore be assumed that their occurrence indicates the presence of these soil types within the range of beehive location.

Published
2024
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49. Effective Transport Recovery of Palladium(II) from Hydrochloric Acid Solutions Using Polymer Inclusion Membrane with Tetrabutylammonium Bromide

Author

Beata Pospiech

Subjects
polymer inclusion membranes (PIMs), palladium(II), tetrabutylammonium bromide (TBAB), separation processes, Organic chemistry, QD241-441
Abstract

This article reports on the extraction of palladium(II) from hydrochloric acid (HCl) solutions using polymer inclusion membranes (PIMs) containing tetrabutylammonium bromide (TBAB) as the ion carrier. The membranes were based on cellulose triacetate (CTA) as the polymer support. The main aim of this study is to determine the possibility of TBAB’s application as the effective ion carrier/extractant of Pd(II) from hydrochloric acid solutions. At first, the effect of the hydrochloric acid concentration in the aqueous phase on palladium(II) extraction was investigated. Next, cellulose triacetate membranes with TBAB as the carrier were prepared and applied for the recovery of Pd(II) from HCl solutions. As a result of the investigations, the optimal composition of the receiving phase was determined to be 0.5 M thiourea in 0.1 M hydrochloric acid. The effect of the acid concentration in the source phase was investigated. The results show a linear decrease in the permeability coefficient and initial flux of palladium(II) with an increase in the hydrochloric acid concentration in the source phase. The separation of Pd(II) from Pt(IV) from the hydrochloric acid solution was also studied. The transport rate of Pd(II) was higher than Pt(IV). The separation coefficient SPd/Pt was 1.3. The results show that transport through PIMs with TBAB can be used as an effective method to recover Pd(II) from hydrochloric acid, especially at a low concentration of this acid.

Published
2024
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50. Effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages containing chitosan, by the ELISA method

Author

L. Havlová, M. Pospiech, Z. Javůrková, M. Bartlová, K. Těšíková, D. Dordevic, S. Dordevic, J. Zemancová, and B. Tremlová

Subjects
Edible packaging, chitosan, grape extract, tropomyosin, ELISA, immunoreactivity, Agriculture (General), S1-972, Immunologic diseases. Allergy, RC581-607
Abstract

ABSTRACTThe aim of this study was evaluated the effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages based on chitosan using the ELISA. The analysed protein was the tropomyosin. The results confirmed the presence of the tropomyosin (3.77 ± 0.79–5.75 ± 0.01 µg/g) in control samples. This study demonstrated that the bioactive substances in the form of grape (0.61 ± 0.34–0.43 ± 0.16 µg/g), blueberry (0.58 ± 0.32–0.39 ± 0.27 µg/g), and parsley extracts (2.09 ± 1.28–0.79 ± 0.40 µg/g) reduces immunoreactivity (p 0.05) on immunoreactivity. ZnO and TiO2 nanoparticles also demonstrated immunoreactivity reduction (p

Published
2023
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