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22 results on '"Postnikov, Alexander B."'

1. Specific cleavage of IGFBP-4 by papp-a in nervous tissue

Author

Dya, German A., Lebedeva, Olga S., Gushchevarov, Daniil A., Volovikov, Egor A., Belikova, Lilia D., Kopylova, Irina V., Postnikov, Alexander B., Artemieva, Marina M., Medvedeva, Natalia A., Lagarkova, Maria A., Katrukha, Alexey G., and Serebryanaya, Daria V.

Published
2024
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3. Cytokine production in an ex vivo model of SARS-CoV-2 lung infection.

Author

Vorobyeva, Daria A., Potashnikova, Daria M., Maryukhnich, Elena V., Rusakovich, George I., Tvorogova, Anna V., Kalinskaya, Anna I., Pinegina, Natalia V., Kovyrshina, Anna V., Dolzhikova, Inna V., Postnikov, Alexander B., Rozov, Fedor N., Sotnikova, Tatiana N., Kanner, Dmitry Yu., Logunov, Denis Yu., Gintsburg, Alexander L., Vasilieva, Elena J., and Margolis, Leonid B.

Subjects
COVID-19, TISSUE culture, VIRUS diseases, LUNG infections, VIRAL load
Abstract

Introduction: The mechanisms of the SARS-CoV-2-triggered complex alterations in immune cell activation and production of cytokines in lung tissue remain poorly understood, in part because of the limited use of adequate tissue models that simulate the structure and cell composition of the lung in vivo. We developed a novel ex vivo model of SARS-CoV-2 infection of lung explants, that maintains the intact tissue composition and the viral load for up to 7-10 days. Using this model, we studied cytokine production during SARS-CoV-2 infection. Materials and methods: Lung tissue was monitored for viability and cell composition using flow cytometry and histological analysis. SARS-CoV-2 infection was verified immunohistochemically, viral loads in tissue and culture medium were monitored by qPCR. A panel of 41 cytokines was measured in culture medium using xMAP technology. Results: The explant lung tissue was viable and maintained viral infection that influenced the cytokine production. Elevated concentrations of G-CSF, GM-CSF, GRO-a, IFN-g, IL-6, IL-8, IP-10, MCP-3, MIP-1a, PDGF-AA, and VEGF, and decreased IL-1RA concentration were observed in infected tissue compared to non-infected tissue. Discussion: Our results generally reflect the data obtained in COVID-19 patients. GRO-a, IFN-g, IL-6, IL-8, MCP-1, MCP-3, and RANTES correlated with the viral load, forming a distinct pro-inflammatory cluster. Thus, our lung ex vivo model faithfully reproduces some aspects of cytokine alterations in COVID-19 patients at an early disease stage, making the investigation of SARS-CoV-2 infection mechanisms more accessible and providing a potential platform for antiviral drug testing. [ABSTRACT FROM AUTHOR]

Published
2024
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11. High throughput testing of drug library substances and monoclonal antibodies for capacity to reduce formation of cystatin C dimers to identify candidates for treatment of hereditary cystatin C amyloid angiopathy.

Author

Östner, Gustav, Lindström, Veronica, Postnikov, Alexander B, Solovyeva, Tatiana I, Emilsson, Ossur I, Grubb, Anders, Östner, Gustav, Lindström, Veronica, Postnikov, Alexander B, Solovyeva, Tatiana I, Emilsson, Ossur I, and Grubb, Anders

Abstract

Objective. To establish a high-throughput system for testing the ability of drugs or monoclonal antibodies to reduce the in vitro formation of cystatin C dimers to identify substances potentially useful for treatment of patients with hereditary cystatin C amyloid angiopathy (HCCAA). Methods. Various combinations of incubation temperature, time period, guanidinium chloride concentration and concentration of cystatin C monomers were tested in low-volume formats to induce dimer formation of recombinant cystatin C. The extent of dimerization was analysed by gel filtration chromatography and agarose gel electrophoresis. Results. A high-throughput system based upon agarose gel electrophoresis was developed and used to test 1040 drugs in a clinical drug library for their capacity to reduce cystatin C dimer formation in vitro. Seventeen substances reducing dimer formation by more than 30% were identified. A similar system for testing the capacity of monoclonal antibodies against cystatin C to reduce the in vitro formation of cystatin C dimers was also developed and used to test a panel of 12 monoclonal antibodies. Seven antibodies reducing dimer formation by more than 30% were identified and the two most potent, Cyst28 and HCC3, reduced dimerization by 75 and 60%, respectively. Conclusion. We constructed a simple high-throughput system for testing the capacity of drugs and monoclonal antibodies to reduce the in vitro formation of cystatin C dimers and several candidates for treatment of HCCAA could be identified.

Published
2011

19. Immunodetection of Glycosylated NT-proBNP Circulating in Human Blood

Author

Seferian, Karina R, primary, Tamm, Natalia N, primary, Semenov, Alexander G, primary, Tolstaya, Anastasia A, primary, Koshkina, Ekaterina V, primary, Krasnoselsky, Mihail I, primary, Postnikov, Alexander B, primary, Serebryanaya, Daria V, primary, Apple, Fred S, primary, Murakami, MaryAnn M, primary, and Katrukha, Alexey G, primary

Published
2008
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