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There are substantial concerns about impaired honey bee health and colony losses due to several poorly understood factors. We used MALDI profiling (MALDI BeeTyping®) analysis to investigate how some environmental and management factors were related to the haemolymph peptidome (all peptides in the circulatory fluid), which reflects the immune status of Apis mellifera, under field conditions across Europe. Honey bees were exposed to varying environmental stressors across eight European countries totalling 128 agricultural sites, reflecting two different crop systems [oilseed rape (OSR) and apple (APP)]. Molecular signatures of haemolymph and the presence/absence of molecular-related ions of three immunity markers, namely the antimicrobial peptides (AMP) Apidaecin, Abaecin and Defensin-1, allowed discrimination of bee responses by country, crop type and site. However, many sites showed no significant signature related to the presence of AMP markers. Conversely, in Sweden (SWE), molecular ion intensities were very high, including those of the AMP markers. Even the lowest values were always higher than in other countries. Furthermore, all experimental sites in SWE expressed AMPs. A machine learning model was developed to discriminate the haemolymphs of bees from APP and OSR sites. The model was 90.6 % accurate in identifying the crop type from the samples used to build the model. Overall, MALDI BeeTyping® on individual bee haemolymph represents an attractive and promising “blood test” for monitoring the impact of stressors on bee health at the landscape scale, thus providing policymakers with new monitoring and regulatory tools.

Staphylococcus lugdunensis is a coagulase-negative Staphylococcus that emerges as an important opportunistic pathogen. However, little is known about the regulation underlying the transition from commensal to virulent state. Based on knowledge of S. aureus virulence, we suspected that the agr quorum sensing system may be an important determinant for the pathogenicity of S. lugdunensis. We investigated the functions of the transcriptional regulator AgrA using the agrA deletion mutant. AgrA played a role in cell pigmentation: DargA mutant colonies were white while the parental strains were slightly yellow. Compared with the wild-type strain, the DargA mutant was affected in its ability to form biofilm and was less able to survive in mice macrophages. Moreover, the growth of DagrA was significantly reduced by the addition of 10% NaCl or 0.4 mM H2O2 and its survival after 2 h in the presence of 1 mM H2O2 was more than 10-fold reduced. To explore the mechanisms involved beyond these phenotypes, the DagrA proteome and transcriptome were characterized by mass spectrometry and RNA-Seq. We found that AgrA controlled several virulence factors as well as stress-response factors, which are well correlated with the reduced resistance of the DagrA mutant to osmotic and oxidative stresses. These results were not the consequence of the deregulation of RNAIII of the agr system, since no phenotype or alteration of the proteomic profile has been observed for the DRNAIII mutant. Altogether, our results highlighted that the AgrA regulator of S. lugdunensis played a key role in its ability to become pathogenic.

The most recent comprehensive database of Martian impact craters was the result of the work of impact crater scientists (S.J. Robbins and B.M. Hynek) who carefully examined the available high-resolution imagery of Mars. Building on this previous work, we present the result of an alternative approach involving 56 planetary scientists and trained students. A web platform was designed for this purpose. All impact craters larger than 1 km in diameter were classified according to a simplified classification scheme, recording the primary or secondary nature of the crater, and the morphology of the ejecta (single, double, or multiple layered ejecta rampart sinuous [LERS], or low-aspect-ratio layer ejecta [LARLE]). In total, 8445 LERS craters, 24,530 partially buried craters, 55,309 secondary craters, and 288,155 craters in the category “standard” were identified. Our assessment differs for 8145 entries in the original database compiled by Robbins and Hynek, which are not considered to be impact structures. In this work, ~39,000 secondary craters have been associated with 108 primary craters. Coupled to the existing database, the database we propose here offers a complementary way to investigate the geological history of Mars. More specifically, the completion of layered ejecta crater morphologies down to 1 km and the connection established between secondary and primary impact crater sources will allow the implementation of statistical studies to reveal the spatial and temporal evolution of the impacted material characteristics. Thanks to the simplified classification we performed here, this version of the database can be easily used as a training data set for crater identification algorithms based on machine-learning techniques with the aim to identify smaller impact craters and to automatically define their morphological characteristics. Since it is not possible to confirm an impact structure from remote-sensing data alone, any Martian impact database at this stage remain

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) fixes atmospheric CO2 into organic compounds and is composed of eight copies each of a large subunit (RbcL) and a small subunit (RbcS). Recent reports have revealed unusual RbcS, which are expressed in particular tissues and confer higher catalytic rate, lesser affinity for CO2, and a more acidic profile of the activity versus pH. The resulting Rubisco was proposed to be adapted to a high CO2 environment and recycle CO2 generated by the metabolism. These RbcS belong to a cluster named T (for trichome), phylogenetically distant from cluster M, which gathers well-characterized RbcS expressed in mesophyll or bundle-sheath tissues. Cluster T is largely represented in different plant phyla, including pteridophytes and bryophytes, indicating an ancient origin., SCOPUS: re.j, info:eu-repo/semantics/published

Contains fulltext : 136409.pdf (publisher's version ) (Open Access), The innate immune system differentially recognizes Candida albicans yeast and hyphae. It is not clear how the innate immune system effectively discriminates between yeast and hyphal forms of C. albicans. Glucans are major components of the fungal cell wall and key fungal pathogen-associated molecular patterns. C. albicans yeast glucan has been characterized; however, little is known about glucan structure in C. albicans hyphae. Using an extraction procedure that minimizes degradation of the native structure, we extracted glucans from C. albicans hyphal cell walls. (1)H NMR data analysis revealed that, when compared with reference (1-->3,1-->6) beta-linked glucans and C. albicans yeast glucan, hyphal glucan has a unique cyclical or "closed chain" structure that is not found in yeast glucan. GC/MS analyses showed a high abundance of 3- and 6-linked glucose units when compared with yeast beta-glucan. In addition to the expected (1-->3), (1-->6), and 3,6 linkages, we also identified a 2,3 linkage that has not been reported previously in C. albicans. Hyphal glucan induced robust immune responses in human peripheral blood mononuclear cells and macrophages via a Dectin-1-dependent mechanism. In contrast, C. albicans yeast glucan was a much less potent stimulus. We also demonstrated the capacity of C. albicans hyphal glucan, but not yeast glucan, to induce IL-1beta processing and secretion. This finding provides important evidence for understanding the immune discrimination between colonization and invasion at the mucosal level. When taken together, these data provide a structural basis for differential innate immune recognition of C. albicans yeast versus hyphae.