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12 results on '"Poulain, Florian"'

1. The APOBEC3B cytidine deaminase is an adenovirus restriction factor

Author

Lejeune, Noémie, primary, Mathieu, Sarah, additional, Decloux, Alexandra, additional, Poulain, Florian, additional, Blockx, Zoé, additional, Raymond, Kyle A., additional, Willemart, Kévin, additional, Vartanian, Jean-Pierre, additional, Suspène, Rodolphe, additional, and Gillet, Nicolas A., additional

Published
2023
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2. Human papillomavirus E6/E7 oncoproteins promote radiotherapy-mediated tumor suppression by globally hijacking host DNA damage repair

Author

Bruyere, Diane, primary, Roncarati, Patrick, additional, Lebeau, Alizee, additional, Lerho, Thomas, additional, Poulain, Florian, additional, Hendrick, Elodie, additional, Pilard, Charlotte, additional, Reynders, Celia, additional, Ancion, Marie, additional, Luyckx, Margaux, additional, Renard, Michael, additional, Jacob, Yves, additional, Twizere, Jean-Claude, additional, Peiffer, Raphael, additional, Peulen, Olivier, additional, Delvenne, Philippe, additional, Hubert, Pascale, additional, McBride, Alison, additional, Gillet, Nicolas, additional, Masson, Murielle, additional, and Herfs, Michael, additional

Published
2023
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5. SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

Author

Coupeau, Damien, primary, Burton, Nicolas, additional, Lejeune, Noémie, additional, Loret, Suzanne, additional, Petit, Astrid, additional, Pejakovic, Srdan, additional, Poulain, Florian, additional, Bonil, Laura, additional, Trozzi, Gabrielle, additional, Wiggers, Laetitia, additional, Willemart, Kévin, additional, André, Emmanuel, additional, Laenen, Lies, additional, Cuypers, Lize, additional, Van Ranst, Marc, additional, Bogaerts, Pierre, additional, Muylkens, Benoît, additional, and Gillet, Nicolas Albert, additional

Published
2020
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7. Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models

Author

Benyelles, Maname, Episkopou, Charikleia, O’Donohue, Marie-Françoise, Kermasson, Laëtitia, Frange, Pierre, Poulain, Florian, Burcu Belen, Fatma, Polat, Meltem, Bole-Feysot, Christine, Langa-Vives, Francina, Gleizes, Pierre-Emmanuel, de Villartay, Jean-Pierre, Callebaut, Isabelle, Decottignies, Anabelle, Revy, Patrick, and UCL - SSS/DDUV/GEPI - Epigénétique

Subjects
p53, Male, protein p53, TPP1 gene, Høyeraal–Hreidarsson syndrome, dyskeratosis congenita, preschool child, fibroblast, TRF1 gene, Mice, genetic stability, telomere length, animal, genetics, gene mutation, microcephaly, rRNA, telomere homeostasis, POT1 gene, poly(A)-specific ribonuclease, TRF2 gene, Mice, Knockout, child, clinical article, telomere, Fetal Growth Retardation, messenger RNA, intrauterine growth retardation, PARN gene, female, priority journal, Child, Preschool, ribosome RNA, enzyme deficiency, down regulation, shelterin, PARN, animal experiment, Article, gene knockout, exoribonuclease, Intellectual Disability, Animals, Humans, controlled study, human, gene, chromosomal parameters, mouse, nonhuman, polynucleotide adenylyltransferase, human cell, disease model, intellectual impairment, genetic transcription, biogenesis, school child, infant, Disease Models, Animal, Dyskeratosis Congenita/genetics/*metabolism/pathology, Exoribonucleases/*deficiency/metabolism, Female, Fetal Growth Retardation/genetics/*metabolism/pathology, Intellectual Disability/genetics/*metabolism/pathology, Microcephaly/genetics/*metabolism/pathology, RNA, Ribosomal/*biosynthesis/genetics, Telomere/genetics/*metabolism/pathology, Telomere Homeostasis, RAP1 gene, RNA, Ribosomal, Exoribonucleases, gene expression, pathology, biosynthesis, knockout mouse, metabolism
Abstract

PARN, poly(A)-specific ribonuclease, regulates the turnover of mRNAs and the maturation and stabilization of the hTR RNA component of telomerase. Biallelic PARN mutations were associated with Høyeraal–Hreidarsson (HH) syndrome, a rare telomere biology disorder that, because of its severity, is likely not exclusively due to hTR down-regulation. Whether PARN deficiency was affecting the expression of telomere-related genes was still unclear. Using cells from two unrelated HH individuals carrying novel PARN mutations and a human PARN knock-out (KO) cell line with inducible PARN complementation, we found that PARN deficiency affects both telomere length and stability and down-regulates the expression of TRF1, TRF2, TPP1, RAP1, and POT1 shelterin transcripts. Down-regulation of dyskerin-encoding DKC1 mRNA was also observed and found to result from p53 activation in PARN-deficient cells. We further showed that PARN deficiency compromises ribosomal RNA biogenesis in patients' fibroblasts and cells from heterozygous Parn KO mice. Homozygous Parn KO however resulted in early embryonic lethality that was not overcome by p53 KO. Our results refine our knowledge on the pleiotropic cellular consequences of PARN deficiency. © 2019 The Authors. Published under the terms of the CC BY 4.0 license

Published
2019

8. Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models

Author

Benyelles, Maname, Episkopou, Harikleia, O'Donohue, Marie-Françoise, Kermasson, Laetitia, Frange, Pierre, Poulain, Florian, Burcu Belen, Fatma, Polat, Meltem, Bole-Feysot, Christine, Langa-Vives, Francina, Gleizes, Pierre-Emmanuel, de Villartay, Jean-Pierre, Callebaut, Isabelle, Decottignies, Anabelle, Revy, Patrick, Laboratoire de Biologie Moléculaire de la Cellule (LBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Université catholique de Louvain, Institut des Sciences de la Vie, Imagine - Institut des maladies génétiques (IMAGINE - U1163), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Genetic and Epigenetic Alterations of Genomes (GEAG), Université Catholique de Louvain (UCL)-de Duve Institute, Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Genome dynamics in the immune system (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Université Catholique de Louvain = Catholic University of Louvain (UCL), Laboratoire de biologie moléculaire eucaryote (LBME), Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Infection à VIH, réservoirs, diversité génétique et résistance aux antirétroviraux (ARV) (EA 7327), Université Paris Descartes - Paris 5 (UPD5), Başkent University Hospital [Adana, Turkey], Pamukkale University, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre d'Ingénierie génétique murine - Mouse Genetics Engineering Center (CIGM), Institut Pasteur [Paris], This work has been supported by institutional grants from INSERM, Ligue Nationale contre le Cancer (Equipe Labellisée La Ligue), Institut National du Cancer INCa, GIS‐Institut des maladies rares, and FNRS (Fonds National de la Recherche Scientifique, Belgium). P.E.G. and M.F.O. are supported by Agence Nationale de la Recherche (ANR 2015 AAP générique CE12‐0001‐DBA Multigenes), and the EuroDBA project is funded by the ERA‐NET program E‐RAR3 (ANR‐15‐RAR3‐0007‐04). P.R. and M.F.O. are scientists from Centre National de la Recherche Scientifique (CNRS). H.E. and F.P. were supported by grants from the Télévie/FNRS and FRIA/FNRS. A.D. is a scientist from the FNRS., ANR-15-CE12-0001,DBA-MULTIGENES,DBA-MULTIGENES: identification et caractérisation de gènes candidats pour la découverte de nouveaux mécanismes physiopathologiques à l'origine de l'anémie de Blackfan-Diamond.(2015), ANR-15-RAR3-0007,EuroDBA,The European Diamond-Blackfan Anemia Consortium(2015), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP), O'Donohue, Marie-Françoise, DBA-MULTIGENES: identification et caractérisation de gènes candidats pour la découverte de nouveaux mécanismes physiopathologiques à l'origine de l'anémie de Blackfan-Diamond. - - DBA-MULTIGENES2015 - ANR-15-CE12-0001 - AAPG2015 - VALID, The European Diamond-Blackfan Anemia Consortium - - EuroDBA2015 - ANR-15-RAR3-0007 - E-Rare-3 - VALID, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, p53, Medicine (General), [SDV]Life Sciences [q-bio], Høyeraal–Hreidarsson syndrome, QH426-470, MESH: Mice, Knockout, Chromatin, Epigenetics, Genomics & Functional Genomics, Shelterin Complex, Mice, MESH: Animals, rRNA, ComputingMilieux_MISCELLANEOUS, Mice, Knockout, Fetal Growth Retardation, Articles, Telomere, Child, Preschool, MESH: Exoribonucleases, Microcephaly, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Female, shelterin, PARN, MESH: Fetal Growth Retardation, Telomere-Binding Proteins, Høyeraal-Hreidarsson syndrome, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH: Microcephaly, Dyskeratosis Congenita, Article, MESH: Telomere Homeostasis, MESH: Intellectual Disability, R5-920, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Intellectual Disability, Genetics, Animals, Humans, MESH: Mice, MESH: Humans, MESH: Child, Preschool, Telomere Homeostasis, MESH: Male, Disease Models, Animal, MESH: Dyskeratosis Congenita, RNA, Ribosomal, Exoribonucleases, MESH: RNA, Ribosomal, Genetics, Gene Therapy & Genetic Disease, MESH: Disease Models, Animal, MESH: Telomere, MESH: Female
Abstract

International audience; PARN, poly(A)-specific ribonuclease, regulates the turnover of mRNAs and the maturation and stabilization of the hTR RNA component of telomerase. Biallelic PARN mutations were associated with Høyeraal-Hreidarsson (HH) syndrome, a rare telomere biology disorder that, because of its severity, is likely not exclusively due to hTR down-regulation. Whether PARN deficiency was affecting the expression of telomere-related genes was still unclear. Using cells from two unrelated HH individuals carrying novel PARN mutations and a human PARN knock-out (KO) cell line with inducible PARN complementation, we found that PARN deficiency affects both telomere length and stability and down-regulates the expression of TRF1, TRF2, TPP1, RAP1, and POT1 shelterin transcripts. Down-regulation of dyskerin-encoding DKC1 mRNA was also observed and found to result from p53 activation in PARN-deficient cells. We further showed that PARN deficiency compromises ribosomal RNA biogenesis in patients' fibroblasts and cells from heterozygous Parn KO mice. Homozygous Parn KO however resulted in early embryonic lethality that was not overcome by p53 KO. Our results refine our knowledge on the pleiotropic cellular consequences of PARN deficiency.

Published
2018

9. Impaired telomere integrity and rRNA biogenesis in PARN‐deficient patients and knock‐out models

Author

Benyelles, Maname, primary, Episkopou, Harikleia, additional, O'Donohue, Marie‐Françoise, additional, Kermasson, Laëtitia, additional, Frange, Pierre, additional, Poulain, Florian, additional, Burcu Belen, Fatma, additional, Polat, Meltem, additional, Bole‐Feysot, Christine, additional, Langa‐Vives, Francina, additional, Gleizes, Pierre‐Emmanuel, additional, de Villartay, Jean‐Pierre, additional, Callebaut, Isabelle, additional, Decottignies, Anabelle, additional, and Revy, Patrick, additional

Published
2019
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10. Nuclear Respiratory Factor 1 and endurance exercise promote human telomere transcription

Author

Diman, Aurélie, Boros, Joanna, Poulain, Florian, Rodriguez, Julie, Purnelle, Marin, Episkopou, Charikleia, Deldicque, Louise, Bertrand, Luc, Francaux, Marc, Decottignies, Anabelle, Telomeres, Telomerase and Disease, UCL - SSS/DDUV - Institut de Duve, UCL - SSS/IONS - Institute of NeuroScience, and UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire

Abstract

DNA breaks activate the DNA damage response and, if left unrepaired, trigger cellular senescence. Telomeres are specialized nucleoprotein structures that protect chromosome ends from persistent DNA damage response activation. Whether protection can be enhanced to counteract the age-dependent decline in telomere integrity is a challenging question. TElomeric Repeat-containing RNA, TERRA, transcribed from telomeres, emerged as important players in telomere integrity. How human telomere transcription is regulated is however still largely unknown. Here, we identify Nuclear Respiratory Factor 1 and Peroxisome proliferator-activated receptor γ Coactivator 1α as regulators of human telomere transcription. Agreeing with an upstream regulation of these factors by AMP-activated protein kinase (AMPK), pharmacological activation of AMPK in cancer cell lines or normal non-proliferating myotubes up-regulated TERRA, thereby linking metabolism to telomere fitness. Importantly, cycling endurance exercise, associated with AMPK activation, increased TERRA levels in skeletal muscle biopsies obtained from ten healthy young volunteers. The data support the idea that exercise may protect against aging.

Published
2016

12. Etat des lieux des peuplements de poisson dans les eaux de transition du bassin Adour Garonne

Author

Lepage, Mario, Girardin, Michel, Durozoï, B., Boët, Philippe, De Maisonneuve, L., Poulain, Florian, Gonthier, Paul, Irstea Publications, Migration, Ecosystèmes estuariens et poissons migrateurs amphihalins (UR EPBX), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), irstea, and Agence de l'eau Adour Garonne

Subjects
ADOUR GARONNE BASSIN, [SDE] Environmental Sciences, [SDE]Environmental Sciences
Abstract

Dans le cadre du contrôle de surveillance imposé par la Directive Cadre européenne sur l'eau (DCE), les Etats membres doivent contrôler tous les éléments de qualité biologique, physico-chimique et hydromorphologique des eaux de transition. L'objectif de cette étude est de réaliser une première étude complète de toutes les masses d'eau de transition du district Adour Garonne ayant pour but d'établir une base quantitative d'information sur la composition et la répartition des peuplements de poisson.

Published
2007

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