Search

Your search keyword '"Pozzi, Deleana"' showing total 39 results
Start Over Author "Pozzi, Deleana"
39 results on '"Pozzi, Deleana"'

1. UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study

Author

Pozzi, Deleana, Grimaldi, Paola, Gaudenzi, Silvia, Di Giambattista, Lucia, Silvestri, Ida, Morrone, Stefania, and Castellano, Agostina Congiu

Subjects
Physics - Biological Physics
Abstract

We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290-320 nm) at a dose of 310 mJ/cm^2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I beta-sheet component and the area of the 1083 cm^-1 band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.

Published
2010

15. Ultrasound well below the intensity threshold of cavitation can promote efficient uptake of small drug model molecules in fibroblast cells

Author

Domenici, Fabio, primary, Giliberti, Claudia, additional, Bedini, Angelico, additional, Palomba, Raffaele, additional, Luongo, Fabio, additional, Sennato, Simona, additional, Olmati, Cristina, additional, Pozzi, Deleana, additional, Morrone, Stefania, additional, Congiu Castellano, Agostina, additional, and Bordi, Federico, additional

Published
2013
Full Text
View/download PDF

16. Non Ionising Radiation as a Non Chemical Strategy in Regenerative Medicine: Ca2+-ICR “In Vitro” Effect on Neuronal Differentiation and Tumorigenicity Modulation in NT2 Cells

Author

Ledda, Mario, primary, Megiorni, Francesca, additional, Pozzi, Deleana, additional, Giuliani, Livio, additional, D’Emilia, Enrico, additional, Piccirillo, Sara, additional, Mattei, Cristiana, additional, Grimaldi, Settimio, additional, and Lisi, Antonella, additional

Published
2013
Full Text
View/download PDF

17. Nonionizing Radiation as a Noninvasive Strategy in Regenerative Medicine: The Effect of Ca2+-ICR on Mouse Skeletal Muscle Cell Growth and Differentiation

Author

De Carlo, Flavia, primary, Ledda, Mario, additional, Pozzi, Deleana, additional, Pierimarchi, Pasquale, additional, Zonfrillo, Manuela, additional, Giuliani, Livio, additional, D'Emilia, Enrico, additional, Foletti, Alberto, additional, Scorretti, Riccardo, additional, Grimaldi, Settimio, additional, and Lisi, Antonella, additional

Published
2012
Full Text
View/download PDF

18. Ion Cyclotron Resonance as a Tool in Regenerative Medicine

Author

Lisi, Antonella, primary, Ledda, Mario, additional, de Carlo, Flavia, additional, Pozzi, Deleana, additional, Messina, Elisa, additional, Gaetani, Roberto, additional, Chimenti, Isotta, additional, Barile, Lucio, additional, Giacomello, Alessandro, additional, D'Emilia, Enrico, additional, Giuliani, Livio, additional, Foletti, Alberto, additional, Patti, Annamaria, additional, Vulcano, Antonella, additional, and Grimaldi, Settimio, additional

Published
2008
Full Text
View/download PDF

24. Morphological and biochemical analysis by atomic force microscopy and scanning near-field optical microscopy techniques of human keratinocytes (HaCaT) exposed to extremely low frequency 50 Hz magnetic field

Author

Rieti, Sabrina, primary, Manni, Vanessa, additional, Lisi, Antonella, additional, Grimaldi, Settimio, additional, Generosi, Renato, additional, Luce, Marco, additional, Perfetti, Paolo, additional, Cricenti, Antonio, additional, Pozzi, Deleana, additional, and Giuliani, Livio, additional

Published
2002
Full Text
View/download PDF

27. Non Ionising Radiation as a Non Chemical Strategy in Regenerative Medicine: Ca2+-ICR “In Vitro” Effect on Neuronal Differentiation and Tumorigenicity Modulation in NT2 Cells.

Author

Ledda, Mario, Megiorni, Francesca, Pozzi, Deleana, Giuliani, Livio, D’Emilia, Enrico, Piccirillo, Sara, Mattei, Cristiana, Grimaldi, Settimio, and Lisi, Antonella

Subjects
REGENERATIVE medicine, NEURONS, CELL differentiation, NEOPLASTIC cell transformation, CANCER cells, ELECTROMAGNETIC radiation, PLURIPOTENT stem cells, TRANSFORMING growth factors-beta
Abstract

In regenerative medicine finding a new method for cell differentiation without pharmacological treatment or gene modification and minimal cell manipulation is a challenging goal. In this work we reported a neuronal induced differentiation and consequent reduction of tumorigenicity in NT2 human pluripotent embryonal carcinoma cells exposed to an extremely low frequency electromagnetic field (ELF-EMF), matching the cyclotron frequency corresponding to the charge/mass ratio of calcium ion (Ca2+-ICR). These cells, capable of differentiating into post-mitotic neurons following treatment with Retinoic Acid (RA), were placed in a solenoid and exposed for 5 weeks to Ca2+-ICR. The solenoid was installed in a μ-metal shielded room to avoid the effect of the geomagnetic field and obtained totally controlled and reproducible conditions. Contrast microscopy analysis reveled, in the NT2 exposed cells, an important change in shape and morphology with the outgrowth of neuritic-like structures together with a lower proliferation rate and metabolic activity alike those found in the RA treated cells. A significant up-regulation of early and late neuronal differentiation markers and a significant down-regulation of the transforming growth factor-α (TGF-α) and the fibroblast growth factor-4 (FGF-4) were also observed in the exposed cells. The decreased protein expression of the transforming gene Cripto-1 and the reduced capability of the exposed NT2 cells to form colonies in soft agar supported these last results. In conclusion, our findings demonstrate that the Ca2+-ICR frequency is able to induce differentiation and reduction of tumorigenicity in NT2 exposed cells suggesting a new potential therapeutic use in regenerative medicine. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

31. Early Events of Fusion Between Epstein Barr Virus and Human Lymphoblastoid Cells (Raji) Detected by R18 Fluorescence Dequenching Measurements

Author

Pozzi, Deleana, Zompetta, Claudia, Faggioni, Alberto, Lisi, Antonella, De Ros, Ivan, Ravagnan, Giampietro, and Grimaldi, Settimio

Abstract

Relief of fluorescence self-quenching was used to monitor fusion (14) of Epstein Barr virus (EBV) with Raji cells after exposure of the virus to a variety of experimental conditions such as neutral or low pH, enzymatic modification of the viral spike glycoproteins, or inhibition of the protein kinase C (PKC) activity. Incubation of the virus at pH 5.9 prior to the binding to the cell membrane led to a significant enhancement of fusion with the plasma membrane. Treatment of Raji cells with an agent known to elevate the endosomal and lysosomal pH (lysosomotropic agent) (3, 12) partially prevented fusion at neutral pH. Desialylation of EBV significantly reduced the extent of fusion with Raji cells. Protein kinase C inhibitor reduced EBV fusion with Raji cells, while treatment with the tumor promoter and the PKC activator TPA caused an increase in the final extent of fusion. Our results suggest that EBV fuses with lymphoblastoid cells in the endocytic vescicles after being rapidly internalized and that protein kinase C is involved in the process of viral entry into cells.

Published
1990
Full Text
View/download PDF

37. Exposure to a 50Hz electromagnetic field induces activation of the Epstein-Barr virus genome in latently infected human lymphoid cells

Author

Lisi, Antonella, Grimaldi, Settimio, Pozzi, Deleana, Santoro, Nadia, Barbatano, Luigi, Pasquali, Elio, and Serafino, Annalucia

Subjects
DNA, MEDICAL research, ENVIRONMENTAL exposure, RADIATION exposure
Abstract

The EBV genome in latently infected lymphoid cells offers an opportunity to follow effects on the transcriptional and translational product clearly distinguishable from those of the host cell genome. Exposure of Akata cells, a human lymphoid cell line latently infected by the EBV genome, to a 50 Hz EMF resulted in an increased number of cellsexpressing the virus early antigens. This finding provides additional evidence that DNA can be modulated by a magnetic field. [ABSTRACT FROM AUTHOR]

Published
1997

38. Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

Author

Gaudenzi, Silvia, Pozzi, Deleana, Toro, Paolo, Silvestri, Ida, Morrone, Stefania, and Congiu Castellano, Agostina

Abstract

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm-1 and 2800 cm-1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non?destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologies in vivo.

Published
2004
Full Text
View/download PDF

39. Structural and permeability sensitivity of cells to low intensity ultrasound: Infrared and fluorescence evidence in vitro

Author

Fabio Domenici, Stefania Morrone, Raffaele Palomba, Federico Bordi, D. Pozzi, Angelico Bedini, Claudia Giliberti, Ion Udroiu, Agostina Congiu Castellano, Lucia Di Giambattista, Domenici, Fabio, Giliberti, Claudia, Bedini, Angelico, Palomba, Raffaele, Udroiu, Ion, Di Giambattista, Lucia, Pozzi, Deleana, Morrone, Stefania, Bordi, Federico, and Castellano Agostina, Congiu

Subjects
Cell Membrane Permeability, Spectrophotometry, Infrared, Acoustics and Ultrasonics, Membrane permeability, Cells, In Vitro Techniques, Fluorescence, Flow cytometry, chemistry.chemical_compound, Mice, Nuclear magnetic resonance, Cell uptake, FTIR spectroscopy, Fluorescence microscopy, Membrane sonoporation, Ultrasound, Animals, Cells, Cultured, Fibroblasts, Flow Cytometry, Fluoresceins, Microscopy, Fluorescence, Ultrasonics, medicine, Fluorescence microscope, membrane sonoporation, fluorescence microscopy, ftir spectroscopy, ultrasound, cell uptake, Viability assay, Fluorescein, Settore CHIM/02 - Chimica Fisica, Microscopy, Cultured, medicine.diagnostic_test, business.industry, In vitro, chemistry, Permeability (electromagnetism), Spectrophotometry, Biophysics, business, Infrared
Abstract

This work is focused on the in vitro study of the effects induced by medical ultrasound (US) in murine fibroblast cells (NIH-3T3) at a low-intensity of exposure (spatial peak temporal average intensity Ita

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results