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6. Role of the SOS response in the conjugative transfer and genome maintenance of multidrug resistance Salmonella Genomic Island 1

Author

PONS, Marine, Praud, Karine, Da Re, Sandra, Ploy, Marie-Cécile, Cloeckaert, Axel, Doublet, Benoît, DOUBLET, BENOIT, Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Société Française de Microbiologie, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), and Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges

Subjects
[SDV] Life Sciences [q-bio], SOS response, multigrud resistance, [SDV]Life Sciences [q-bio], [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, SGI1, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS, IncC plasmid
Abstract

International audience

Published
2021

12. NGS characterization of expanded-spectrum cephalosporins resistant Morganellaceae spp. from animal and human origins

Author

Laval, Lucie, Praud, Karine, Leclercq, Sébastien, Cloeckaert, Axel, Ploy, Marie-Cécile, Barraud, Olivier, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), INRA, UMR Infectiologie et Santé Publique (ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and DOUBLET, BENOIT

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

16. Identification and Characterization of oriT and Two Mobilization Genes Required for Conjugative Transfer of Salmonella Genomic Island 1

Author

Szabó, Mónika, Hegyi, Anna, Douard, Gregory, Praud, Karine, Nagy, István, Olasz, Ferenc, Cloeckaert, Axel, Kiss, János, and Doublet, Benoît

Subjects
transfert de gène, Microbiology and Parasitology, salmonella, plasmide, résistance aux antibiotiques, ilot génomique, Microbiologie et Parasitologie, salmonella genomic island 1, integrative mobilizable element, IncA/C plasmids, origin of transfer (oriT), horizontal gene transfer, mobile genetic element (MGE), antibiotic resistance (AR)
Abstract

The integrative mobilizable elements of SGI1-family considerably contribute to the spread of resistance to critically important antibiotics among enteric bacteria. Even though many aspects of SGI1 mobilization by IncA and IncC plasmids have been explored, the basic transfer elements such as oriT and self-encoded mobilization proteins remain undiscovered. Here we describe the mobilization region of SGI1 that is well conserved throughout the family and carries the oriTSGI1 and two genes, mpsA and mpsB (originally annotated as S020 and S019, respectively) that are essential for the conjugative transfer of SGI1. OriTSGI1, which is located in the vicinity of the two mobilization genes proved to be a 125-bp GC-rich sequence with several important inverted repeat motifs. The mobilization proteins MpsA and MpsB are expressed from a bicistronic mRNA, although MpsB can be produced from its own mRNA as well. The protein structure predictions imply that MpsA belongs to the lambda tyrosine recombinase family, while MpsB resembles the N-terminal core DNA binding domains of these enzymes. The results suggest that MpsA may act as an atypical relaxase, which needs MpsB for SGI1 transfer. Although the helper plasmid-encoded relaxase proved not to be essential for SGI1 transfer, it appeared to be important to achieve the high transfer rate of the island observed with the IncA/IncC-SGI1 system.

Published
2019

17. Identification and characterization of oriT and two mobilization genes required for conjugative transfer of Salmonella Genomic Island 1

Author

Kiss, János, Szabó, Mónika, Hegyi, Anna, Douard, Gregory, Praud, Karine, Nagy, István, Olasz, Ferenc, Cloeckaert, Axel, Doublet, Benoît, Agricultural Biotechnology Institute, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Hungarian Scientific Research Fund K 105635 and NKFI K 128203, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
Microbiology (medical), salmonella genomic island 1, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, IncA/C plasmids, origin of transfer (oriT), integrative mobilizable element, horizontal gene transfer, antibiotic resistance (AR), Microbiology, mobile genetic element (MGE)
Abstract

International audience; The integrative mobilizable elements of SGI1-family considerably contribute to the spread of resistance to critically important antibiotics among enteric bacteria. Even though many aspects of SGI1 mobilization by IncA and IncC plasmids have been explored, the basic transfer elements such as oriT and self-encoded mobilization proteins remain undiscovered. Here we describe the mobilization region of SGI1 that is well conserved throughout the family and carries the oriTSGI1 and two genes, mpsA and mpsB (originally annotated as S020 and S019, respectively) that are essential for the conjugative transfer of SGI1. OriTSGI1, which is located in the vicinity of the two mobilization genes proved to be a 125-bp GC-rich sequence with several important inverted repeat motifs. The mobilization proteins MpsA and MpsB are expressed from a bicistronic mRNA, although MpsB can be produced from its own mRNA as well. The protein structure predictions imply that MpsA belongs to the lambda tyrosine recombinase family, while MpsB resembles the N-terminal core DNA binding domains of these enzymes. The results suggest that MpsA may act as an atypical relaxase, which needs MpsB for SGI1 transfer. Although the helper plasmid-encoded relaxase proved not to be essential for SGI1 transfer, it appeared to be important to achieve the high transfer rate of the island observed with the IncA/IncC-SGI1 system.

Published
2019
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18. Genome-based insights into the resistome and associated-mobilome of extended-spectrum cephalosporins resistant Morganellaceae spp

Author

Laval, Lucie, Praud, Karine, Leclercq, Sébastien, Cloeckaert, Axel, Ploy, Marie-Cécile, Barraud, Olivier, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), DOUBLET, BENOIT, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV] Life Sciences [q-bio], Morganella morganii, SXT, plasmides IncA/C, [SDV]Life Sciences [q-bio], blaCMY-2, blaVEB-6, élément intégratif et conjugatif, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Proteus mirabilis, SGI1, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract

International audience; Objectif - IntroductionLa résistance aux antibiotiques est une problématique majeure pour laquelle une approche « One Health » est désormais indispensable. L’arsenal génétique des bactéries pour s’échanger des gènes (plasmides et îlots génomiques transférables) est un des éléments responsables de la diffusion de la résistance au sein/entre les différents écosystèmes. Les Morganellaceae sont des bactéries ubiquistes, pathogènes opportunistes pour lesquelles les infections dues à des souches multirésistantes ne cessent de s’accroître. L’épidémiologie et les mécanismes moléculaires de la résistance de cette famille sont à ce jour peu explorée.Matériels (ou Patients) et méthodesUne collection de 83 souches de Morganellaceae constituée majoritairement de Proteus mirabilis et Morganella morganii résistantes aux céphalosporines de dernières générations, a été rassemblée avec des isolats d’origine humaine (France, Bulgarie et Taiwan), et animale (France). L’étude du résistome et du mobilome associé a été réalisée en combinant des approches phénotypiques et génotypiques (séquençage haut-débit). RésultatsToutes les souches étaient multirésistantes (> 3 familles d’antibiotiques). Une analyse phylogénétique a révélé une importante diversité à l’exception de quelques clusters proches. Les b-Lactamases à Spectre Etendu du groupe CTX-M ont été majoritairement identifiées (41/83), ainsi que la céphalosporinase acquise CMY-2 (23/83). Les éléments intégratifs et conjugatifs (ICE/IME) des familles SXT (8/83) et SGI1 (13/83) véhiculant respectivement les gènes blaCMY-2 et blaVEB-6 ont été identifiés, certaines souches possédant un ICE et un IME. Un transfert conjugatif de la résistance aux céphalosporines de dernières générations n’a été observé que pour un tiers des souches. Des analyses bioinformatiques approfondies sont en cours afin de confirmer ces premiers résultats et localiser les gènes de résistance.ConclusionContrairement aux principales espèces d'Entérobactéries, les gènes de résistance BLSE/AmpC ne semblent pas principalement localisés sur des plasmides conjugatifs chez les Morganellaceae. Les plasmides conjugatifs IncA/C ainsi que les éléments intégratifs et conjugatifs appartenant aux familles SXT et SGI1 jouent un rôle important dans leur diffusion parmi les espèces de Morganellaceae.

Published
2018

19. Résistome et mobilome des Morganellaceae résistantes aux céphalosporines de dernières générations

Author

Laval, Lucie, Praud, Karine, Leclercq, Sébastien, Cloeckaert, Axel, Ploy, Marie-Cecile, Barraud, Olivier, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

20. Escherichia coli producteurs de β-lactamases à spectre étendu chez le cheval en France

Author

de Lagarde, Maud, Larrieu, Caroline, Praud, Karine, Lallier, Nathalie, Trotereau, Angélina, Schouler, Catherine, Salle, Guillaume, Arsenault, Julie, Fairbrother, John M, Doublet, Benoît, Laboratoire de référence OIE pour Escherichia coli, Université de Montréal (UdeM), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Faculté de médecine vétérinaire, école de Saint-Hyacinthe, Département de pathologie et microbiologie-épidémiologie, Animaux de la ferm, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, microbiote intestinal, BLSE, [SDV]Life Sciences [q-bio], Cheval
Abstract

International audience; Objectif-Introduction : Les animaux de compagnie sont considérés comme un réservoir potentiel de bactéries résistantes aux antibiotiques notamment aux céphalosporines de dernières générations. L’objectif de cette étude était de déterminer le portage intestinal de souches d’Escherichia coli résistantes aux céphalosporines de dernières générations (C3/4G) chez le cheval en France et de caractériser les déterminants génétiques de cette résistance.Matériels et méthodes : Sept-cent-trente-huit chevaux de 41 élevages et centres équestres français ont été échantillonnés pendant l’été 2015. La présence d’E. coli résistants aux céphalosporines de dernières générations a été recherchée dans les crottins. Les gènes de résistance aux C3/4G ont été déterminéss par PCR et séquençage. Les plasmides porteurs de gènes BLSE et/ou céphalosporinase AmpC ont été transférés par conjugaison ou transformation afin d’être caractérisés par typage de réplicon, profil de restriction et multilocus sequence typing. Les souches d’E. coli resistantes aux C3/4G rations ont été caractérisées par champs pulsé, détermination du phylogroupe et du contenu en gènes de virulence.Results: Seize structures équestres hébergeaient au minimum un cheval porteur d’E. coli résistants aux C3/4G, ce qui représentait une prévalence de 7,9% de portage parmi les chevaux échantillonnés. Cinquante-une souches distinctes d’E. coli résistantes aux C3/4G ont été incluses dans l’analyse moléculaire de la résistance. Toutes ces souches présentaient une grande diversité de profil de macrorestriction en champs pulsé XbaI, appartenaient majoritairement au phylogroupe B1 et ne possédaient pas les gènes majeurs de virulence testés pour les animaux suggérant qu’il s’agit de souches commensales non pathogènes. Les BLSE du groupe CTX-M étaient largement prédominante (blaCTX-M-1, n=35; blaCTX-M-2, n=8; blaCTX-M-14, n=2). Les gènes BLSE/AmpC ont été localisés sur différents plasmides appartenant aux groupes d’incompatibilité IncHI1, IncI1, IncN, et IncY. Les gènes BLSE blaCTX-M-1 et blaCTX-M-2 étaient principalement portés par des plasmides IncHI1. Conclusion: Cette étude originale a mis en évidence un portage significatif d’E. coli producteur de BLSE chez le cheval sain en France. Les plasmides porteurs de gènes BLSE sont les acteurs majeurs de la dissémination de la résistance aux C3/4G dans le microbiote intestinal du cheval.

Published
2017

21. Fecal carriage and characterization of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Escherichia coli from healthy horses in France

Author

de Lagarde, Maud, Larrieu, Caroline, Praud, Karine, Lallier, Nathalie, Trotereau, Angélina, Schouler, Catherine, Salle, Guillaume, Arsenault, Julie, Fairbrother, John M, Doublet, Benoît, Laboratoire de référence OIE pour Escherichia coli, Université de Montréal, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Département d’épidémiologie, Université de Montréal (UdeM), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), University of Veterinary Medicine [Hannover]. DEU., and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, poultry droppings, bacteria, cheval, biochemical phenomena, metabolism, and nutrition, escherichia coli, fecès, horse, équin, beta lactamase
Abstract

Fecal carriage and characterization of extended-spectrum β-lactamase- and AmpC β-lactamase-producing [i]Escherichia coli[/i] from healthy horses in France. 7. Symposium on Antimicrobial Résistance in Animals and the Environment (ARAE)

Published
2017

23. Le microbiote intestinal des chevaux, en France, est-il un réservoir de gènes d’antibiorésistance ? Caractérisation de souches de Escherichia coli multirésistantes aux antibiotiques et productrices de béta-lactamases à spectre étendu (BLSE) et de céphalosporinases (AmpC)

Author

de Lagarde, Maud, Larrieu, Caroline, Praud, Karine, Lallier, Nathalie, Trotereau, Angélina, Schouler, Catherine, Salle, Guillaume, Arsenault, Julie, Fairbrother, John M., Doublet, Benoît, Laboratoire de référence OIE pour Escherichia coli, Université de Montréal, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Département d’épidémiologie, Projet national BIOREQUI financé par l’IFCE, Hippolia, Fond Eperon, Université de Montréal (UdeM), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut Français du Cheval et de l'Equitation (IFCE). FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, BLSE/AmpC, microbiote, ESBL/AmpC, E. coli, antibiorésistance, France, antimicrobialresistance, equine, équin
Abstract

Le microbiote intestinal des chevaux, en France, est-il un réservoir de gènes d’antibiorésistance ? Caractérisation de souches de [i]Escherichia coli[/i] multirésistantes aux antibiotiques et productrices de béta-lactamases à spectre étendu (BLSE) et de céphalosporinases (AmpC). 43. Journée de la Recherche Equine

Published
2017

24. Dominant plasmids carrying extended spectrum β-lactamases blaCTX-M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters

Author

Dupouy-Guiraute, Véronique, Doublet, Benoît, Arpaillange, Nathalie, Praud, Karine, Bibbal, Delphine, Brugère, Hubert, Oswald, Eric, Cloeckaert, Axel, Toutain, Pierre-Louis, Bousquet‐Mélou, Alain, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut de Recherche en Santé Digestive (IRSD ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées, CHU Toulouse [Toulouse], ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ESBL-producing E. coli, plasmid, Escherichia coli, blaCTX-M, slaughterhouse, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, wastewater, WWTP
Abstract

International audience; Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of ESBL-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant E. coli strains, CTX-M distributions and the genetic lineage of blaCTX-M -carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared to that of urban wastewater. The blaCTX-M-1 gene was predominant in slaughterhouse wastewater whereas diverse blaCTX-M genes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying blaCTX-M genes revealed that blaCTX-M-1 and blaCTX-M-15 genes were harbored by the predominant blaCTX-M-1 IncI1/ST3 and blaCTX-M-15 F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant blaCTX-M-1 and blaCTX-M-15 plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation.

Published
2016
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25. A specific SGI1 multidrug resistance region in ciprofloxacin-resistant Salmonella enterica serotype Kentucky from human and poultry sources in Belgium

Author

Doublet, Benoît, Ceyssens, Pieter-Jan, Jasson, Vicky, Mattheus, Wesley, Praud, Karine, Butaye, Patrick, Wattiau, Pierre, Vanhoof, Raymond, Bertrand, Sophie, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Scientific Institute for Public Health, Sciensano [Bruxelles], Réseau International des Instituts Pasteur (RIIP), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

26. Prevalence, risk factors, and characterization of multidrug resistant and extended spectrum β‐lactamase/AmpC β‐lactamase producing Escherichia coli in healthy horses in France in 2015.

Author

Lagarde, Maud, Larrieu, Caroline, Praud, Karine, Schouler, Catherine, Doublet, Benoît, Sallé, Guillaume, Fairbrother, John M., and Arsenault, Julie

Subjects
MULTIDRUG resistance, BETA lactamases, ESCHERICHIA coli, HORSE diseases, AMOXICILLIN
Abstract

Background: Although antimicrobial resistance is increasingly common in equine medicine, molecular and epidemiological data remains scarce. Objectives: We estimated the prevalence of, and risk factors for, shedding of multidrug resistant (MDR), extended spectrum β‐lactamase (ESBL)‐producing, and AmpC β‐lactamase‐producing, or some combination of these in Escherichia coli in horses in France. We characterized ESBL/AmpC isolates for antimicrobial susceptibility and the presence of virulence and ESBL/AmpC‐associated resistance genes. Animals Fecal samples from healthy adult horses at 41 premises were collected. A questionnaire was completed by each premises manager. A subset of these samples was tested to build 2 bacterial collections. Methods: Indicator (without enrichment) and specific (enrichment with ceftriaxone) E. coli tested for antimicrobial susceptibility. Prevalence of isolates nonsusceptible to antimicrobials was estimated at the horse and the premises level. The ESBL/AmpC and virulence genes were identified by PCR. Multivariable logistic regression was used to investigate risk factors for MDR and ESBL/AmpC isolates at premises. Results: Approximately 44% of horses shed MDR E. coli. Resistance most commonly was observed to ampicillin, streptomycin, and amoxicillin/clavulanic acid. Twenty‐nine percent of premises housed horses shedding ESBL/AmpC‐producing isolates. The ESBL/AmpC gene most commonly identified was blaCTX‐M‐1. Virulence gene iutA was identified in 1 ESBL/AmpC‐producing isolate. Medical treatment, staff numbers, and activity were identified as risk factors for housing horses shedding ESBL/AmpC‐producing E. coli isolates. Conclusions and Clinical Importance: Prevalence of healthy horses harboring ESBL/AmpC genes and MDR isolates in their intestinal microbiota is substantial. Risk factors could be used to elaborate guidelines to prevent their dissemination. [ABSTRACT FROM AUTHOR]

Published
2019
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27. Dramatic increase of AmpC- and ESBL- producing d-Tartrate-positive Salmonella enterica serotype Paratyphi B from broilers in Belgium, 2008-2010

Author

Doublet, Benoît, Praud, Karine, Cloeckaert, Axel, Butaye, Patrick, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Sciensano [Bruxelles], Réseau International des Instituts Pasteur (RIIP), Institut National de la Recherche Agronomique (INRA). FRA., ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

28. Complete nucleotide sequence analysis of multidrug-resistance IncA/C plasmid PR55 from Klebsiella pneumoniae isolated in 1969

Author

Doublet, Benoît, Boyd, D., Douard, Gregory, Praud, Karine, Cloeckaert, Axel, Mulvey, M.R., Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Public Health Agency of Canada, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
klebsiella pneumoniae, Bactériologie, plasmide de résistance, Bacteriology, résistance aux antibiotiques, séquence nucléotidique, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract

National audience; Objectives: To determine the complete nucleotide sequence of the multidrug-resistant IncA/C plasmid pR55 from a clinical Klebsiella pneumoniae strain that was isolated from urinary tract infection in 1969 in a French hospital and compare it with those of contemporary emerging IncA/C plasmids. Methods: The plasmid was purified and sequenced using a 454 sequencing approach. After draft assembly, additional PCRs and walking reads were performed for gap closure. Sequence comparisons and multiple alignments with other IncA/C plasmids were done using BLAST algorithm and CLUSTAL W, respectively. Conclusions: IncA/C2 multidrug resistance plasmids possess a broad host-range and have been largely implicated in the dissemination of antibiotic resistance among Enterobacteriaceae from humans and animals. This typical IncA/C2 genetic scaffold appears to carry various multidrug resistance gene arrays and is now also a successful vehicle for spreading of AmpC-like cephalosporinase and metallo-β-lactamase genes such as blaCMY and blaNDM, respectively.

Published
2012

29. Salmonella genomic island 1-J variants associated with Salmonella enterica serovar Kentucky isolates from Asia

Author

Doublet, Benoît, Le Hello, Simon, Praud, Karine, Weill, François-Xavier, Cloeckaert, Axel, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and Institut Pasteur [Paris]

Subjects
[SDV]Life Sciences [q-bio], ILOT GENOMIQUE, VARIANT, ComputingMilieux_MISCELLANEOUS, SEROTYPE KENTUCKY
Abstract

National audience

Published
2011

32. Comparative analysis of IncHI2 plasmids carrying bla(CTX-M-2) or bla(CTX-M-9) from Escherichia coli and salmonella enterica strains isolated from poultry and humans

Author

Fernandez, Aurora Garcia, Cloeckaert, Axel, Bertini, Alessia, Praud, Karine, Doublet, Benoît, Weill, François-Xavier, Carattolil, Alessandra, Istituto Superiore di Sanità (ISS), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Biodiversité des Bactéries Pathogènes Émergentes, Institut Pasteur [Paris] (IP), This work was supported by the MED-VET-NET Contract with the European Commission (FOOD-CT-2004-506122) and the Specific Targeted Research Project 'Role of mobile genetic element in the spread of antimicrobial drug resistance' (contract no. LSHM-CT-2005-018705)., Istituto Superiore di Sanità, and Institut Pasteur [Paris]

Subjects
COMPARATIVE GENOMICS, [SDV]Life Sciences [q-bio], COMPLETE NUCLEOTIDE-SEQUENCE, SPECTRUM BETA-LACTAMASES, CTX-M, FRANCE, MESH: beta-Lactamases, Poultry, beta-Lactamases, EMERGENCE, Belgium, MESH: Belgium, Mechanisms of Resistance, MESH: Plasmids, MESH: Poultry, Escherichia coli, polycyclic compounds, Humans, MESH: Animals, COMMUNITY, RESISTANCE, MESH: Humans, MESH: Escherichia coli, ANIMALS, Salmonella enterica, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Isoenzymes, MESH: France, MESH: Salmonella enterica, MESH: Isoenzymes, VIRCHOW, Plasmids
Abstract

International audience; Salmonella enterica bla(CTX-M-2) and bla(CTX-M-9) plasmid backbones from isolates from Belgium and France were analyzed. The bla(CTX-M-2-)plasmids from both human and poultry isolates were related to the IncHI2 pAPEC-01-R plasmid, previously identified in the United States in avian Escherichia coli strains; the bla(CTX-M-9) plasmids were closely related to the IncHI2 R478 plasmid.

Published
2007
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34. Dissemination of an extended-spectrum-beta-lactamase bla(TEM-52) gene-carrying IncI1 plasmid in various Salmonella enterica serovars isolated from poultry and humans in Belgium and France between 2001 and 2005

Author

Cloeckaert, Axel, Praud, Karine, Doublet, Benoît, Bertini, Alessia, Carattoli, Alessandra, Butaye, Patrick, Imberechts, Hein, Bertrand, Sophie, Collard, Jean-Marc, Arlet, Guillaume, Weill, François-Xavier, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Istituto Superiore di Sanità, Centre d'Etudes Vétérinaires et Agrochimiques, Partenaires INRAE, Scientific Institute for Public Health, and UPRES EA 2392

Subjects
EMERGENCE, RESISTANCE, VIRCHOW, [SDV]Life Sciences [q-bio], IDENTIFICATION, ESCHERICHIA-COLI STRAINS, GENOMIC ISLAND-1, TYPHIMURIUM, biochemical phenomena, metabolism, and nutrition, HEALTHY
Abstract

International audience; We report here the dissemination of a conjugative IncI1 plasmid carrying bla (TEM-52) on a Tn3 transposon conferring resistance to extended-spectrum cephalosporins in Salmonella enterica serovar Agona, Derby, Infantis, Paratyphi B dT(+), and Typhimurium isolates from poultry and humans in Belgium and France from 2001 to 2005. The most prevalent serovar spreading this resistance was serovar Infantis.

Published
2007
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35. Variant Salmonella genomic island 1-L in Salmonella enterica serovar newport

Author

Doublet, Benoît, Praud, Karine, Demartin, M., Weill, François-Xavier, Cloeckaert, Axel, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and Institut Pasteur [Paris]

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

36. Emergence de BLSE dans différents sérotypes de salmonelles en Belgique

Author

Butaye, P, Bertrand, S, Praud, Karine, Imberechts, H, Diereick, K, Collard, Jm, Cloeckaert, Axel, Inconnu, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

37. Clonal emergence of extended-spectrum β-lactamase (CTX-M-2)-producing Salmonella enterica serovar Virchow isolates with reduced susceptibilities to ciprofloxacin among poultry and humans in Belgium and France (2000 to 2003)

Author

Bertrand, Sophie, Weill, François-Xavier, Cloeckaert, Axel, Vrints, Martine, Mairiaux, Eric, Praud, Karine, Dierick, Katlijne, Wildemauve, Christa, Godard, Claudine, Butaye, Patrick, Imberechts, Hein, Grimont, Patrick A.D., Collard, Jean-Marc, Scientific Institute for Public Health, Biologie cellulaire du parasitisme, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Department of Bacteriology and Immunology, Norwegian Institute of Public Health [Oslo] (NIPH), ProdInra, Migration, Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Réseau International des Instituts Pasteur (RIIP), Biodiversité des bactéries pathogènes émergentes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence des Salmonella - Bactéries pathogènes entériques (CNR), Institut Pasteur [Paris] (IP), and Centre d'Étude et de Recherches Vétérinaires et Agrochimiques (CODA-CERVA)

Subjects
DNA, Bacterial, Genotype, Epidemiology, Microbial Sensitivity Tests, Poultry, beta-Lactamases, ciprofloxacine, Bacterial Proteins, Belgium, Ciprofloxacin, Drug Resistance, Multiple, Bacterial, Animals, Cluster Analysis, Humans, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, belgique, Poultry Diseases, Salmonella Infections, Animal, Microbiology and Parasitology, Chromosomes, Bacterial, bacterial infections and mycoses, DNA Fingerprinting, Microbiologie et Parasitologie, Anti-Bacterial Agents, Bacterial Typing Techniques, Electrophoresis, Gel, Pulsed-Field, volaille, Meat Products, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Salmonella Infections, France, humain, salmonella enterica, Genome, Bacterial, Polymorphism, Restriction Fragment Length, Plasmids
Abstract

International audience; Antibiotic treatment is not required in cases of Salmonella enterica gastroenteritis but is essential in cases of enteric fever or invasive salmonellosis or in immunocompromised patients. Although fluoroquinolones and extended-spectrum cephalosporins are the drugs of choice to treat invasive Salmonella, resistance to these antibiotics is increasing worldwide. During the period 2000 to 2003, 90 Salmonella enterica serovar Virchow poultry and poultry product isolates and 11 serovar Virchow human isolates were found to produce an extended-spectrum beta-lactamase, CTX-M-2, concomitantly with a TEM-1 beta-lactamase. The bla(CTX-M-2) gene was located on a large conjugative plasmid (>100 kb). Pulsed-field gel electrophoresis indicated a clonal relationship between the poultry and human isolates. All these isolates displayed additional resistance to trimethoprim-sulfamethoxazole and tetracycline as well as a reduced susceptibility to ciprofloxacin (MICs of between 0.5 and 1 mu g/ml). CTX-M-2-producing Salmonella with a reduced susceptibility to fluoroquinolones constitutes a major concern, since such strains could disseminate on a large scale and jeopardize classical antibiotic therapy in immunocompromised patients.

Published
2006
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38. TolC but not AcrB is essential for multidrug-resistant Salmonella enterica serovar-Typhimurium colonisation of chicks

Author

Baucheron, Sylvie, Mouline, Christian, Praud, Karine, Chaslus-Dancla, E., Cloeckaert, Axel, Bio-Agresseurs, Santé, Environnement [Nouzilly] (UR BASE), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

39. TolC but not AcrB plays an essential role in colonisation of chicks with multi-resistant Salmonella typhimurium DT104

Author

Baucheron, Sylvie, Mouline, Christian, Praud, Karine, Chaslus-Dancla, E., Cloeckaert, Axel, Bio-Agresseurs, Santé, Environnement [Nouzilly] (UR BASE), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], MULTIRESISTANCE, SYSTEME EFFLUX, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

41. QTL analysis for fruit quality traits in octoploid strawberry (Fragaria x ananassa)

Author

Lerceteau-Köhler, E., Moing, Annick, Guérin, G., Renaud, Christel, Courlit, S., Camy, D., Praud, Karine, Parisy, V., Bellec, Fabian, Maucourt, Mickael, Rolin, Dominique, Roudeillac, P., Denoyes-Rothan, Beatrice, Centre Interrégional de Recherche et d'Expérimentation de la Fraise (CIREF), Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), and Unité de recherches Espèces Fruitières et Vigne (UREFV)

Subjects
[SDV]Life Sciences [q-bio], FRAISIER, SELECTION ASSISTEE PAR MARQUEUR, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2003

42. Réalisation d'une carte génétique partielle du fraisier Fragaria x ananassa à l'aide de marqueurs AFLP

Author

Praud, Karine, Unité de recherches Espèces Fruitières et Vigne (UREFV), Institut National de la Recherche Agronomique (INRA), and Département Génie Biologique. Institut Universitaire de Technologie (IUT), La Rochelle, FRA.

Subjects
[SDV]Life Sciences [q-bio], FRAISIER, AMELIORATION DES PLANTES, BIOLOGIE MOLECULAIRE, GENETIQUE
Abstract

*INRA CR Bordeaux, UREFV Diffusion du document : INRA CR Bordeaux, UREFV Diplôme : DUT

Published
2000

43. Dominant plasmids carrying extended-spectrum Β-lactamases blaCTX-M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters.

Author

Dupouy, Véronique, Doublet, Benoît, Arpaillange, Nathalie, Praud, Karine, Bibbal, Delphine, Brugère, Hubert, Oswald, Eric, Cloeckaert, Axel, Toutain, Pierre-Louis, and Bousquet-Mélou, Alain

Subjects
PLASMIDS, INDUSTRIAL wastes, ESCHERICHIA coli, SLAUGHTERING, SEWAGE disposal plants, DRUG resistance in bacteria, AQUATIC resources
Abstract

Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of extended-spectrum β-lactamases (ESBL)-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant Escherichia coli strains, CTX-M distributions and the genetic lineage of blaCTX-M-carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared with that of urban wastewater. The blaCTX-M-1 gene was predominant in slaughterhouse wastewater whereas diverse blaCTX-M genes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying blaCTX-M genes revealed that blaCTX-M-1 and blaCTX-M-15 genes were harboured by the predominant blaCTX-M-1 IncI1/ST3 and blaCTX-M-15 F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant blaCTX-M-1 and blaCTX-M-15 plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation. [ABSTRACT FROM AUTHOR]

Published
2016
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49. Dissemination of an Extended-Spectrum-β-Lactamase bla TEM-52 Gene-Carrying IncI1 Plasmid in Various Salmonella enterica Serovars Isolated from Poultry and Humans in Belgium and France between 2001 and 2005

Author

Cloeckaert, Axel, primary, Praud, Karine, additional, Doublet, Benoît, additional, Bertini, Alessia, additional, Carattoli, Alessandra, additional, Butaye, Patrick, additional, Imberechts, Hein, additional, Bertrand, Sophie, additional, Collard, Jean-Marc, additional, Arlet, Guillaume, additional, and Weill, François-Xavier, additional

Published
2007
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