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14 results on '"Promwee, Athakorn"'

5. Effect of Dissolved Oxygen and Chemical Scarification on Andrographis paniculata Seed Germination in Macrobubble Conditions

Author

Promwee, Athakorn, Islam, Shams Shaila, and Khomphet, Thanet

Abstract

Andrographis paniculata is used in Thai traditional medicine. This plant contains a bitter compound called andrographolide, which is highly effective in the prevention of many diseases. It is an effective treatment for infectious diseases and has a prophylactic effect owing to its powerful immunity-boosting benefits. Recently, it has been widely used to treat COVID-19. However, commercial planting of A. paniculata is performed by seeding, which leads to seed germination problems. The seed germination is relatively low and not efficient under normal conditions for various reasons, such as a combined dormancy of physical and innate nature, the diversity of the seeds in different lots, and the fact that the germination duration was not uniform in the same lot. An easily applied and inexpensive method for farmers to develop mass plantings to stimulate germination is by using macrobubble conditions by aerating seeds in sterile water in collaboration with chemical scarification, which is the idea of creating a hard seed coat that causes seed dormancy to break while root germination occurs at 25°C. Germination was completed after 16 days. The dissolved oxygen (DO) concentrations in this environment were 5, 6, 7, 8, and 9 mg·L−1. The oxygen intensity of 9 mg·L−1 showed the highest germination percentage (26.33%). It was found to be optimal for macrobubble conditions. Seedlings were treated with chemicals (PEG, NaCl, H2SO4, KCl, KNO3, NaHClO3, and GA3) after soaking in macrobubbles with optimum DO. The results showed that NaHClO3 conc. (30 min) showed a generation percentage reaching 92%, which could greatly promote up to 3.63 folds compared with the control in the macrobubble aeration system.

Published
2023
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11. Trichoderma asperellum (NST-009): A Possible Thai Native Antagonistic Fungus for Managing White Root Disease of Rubber Trees (Hevea brasiliensis).

Author

Promwee, Athakorn, Intana, Warin, and Khomphet, Thanet

Subjects
*ROOT diseases, *HEVEA, *ANTAGONISTIC fungi, *TREE diseases & pests, *TRICHODERMA, *RHIZOSPHERE
Abstract

Background: Rigidoporus microporus causes white root disease, which is one of the most harmful diseases in rubber trees in Thailand. The objectives of this study were to determine the efficacy of T. asperellum NST-009 and its antifungal metabolite in inhibiting R. microporus mycelial development and efficacy of T. asperellum NST-009 in controlling white root disease of rubber trees in an open-field house experiment. Methods: Four native strains of T. asperellum from Nakhon Si Thammarat Province and a commercial strain of Thailand were used in this study. This study was conducted at Agricultural Microbial Production and Service Center, Walailak University, Nakhon Si Thammarat, Thailand, during the period 2017-2020. Result: T. asperellum NST-009 significantly inhibited R. microporus mycelial growth by 77.07% in vitro and its antifungal metabolite from the culture filtrate of T. asperellum NST-009 inhibited mycelial growth by 92.31%. T. asperellum NST-009 reduced the disease severity index by 76.38% in the open-field house experiment compared to the inoculated control. Furthermore, T. asperellum NST-009 was found to survive in rhizosphere soil at 4.50105 CFU/g soil and colonized the roots at 100.00%. [ABSTRACT FROM AUTHOR]

Published
2022
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14. Efficacy of indigenous Trichoderma harzianumin controlling Phytophthoraleaf fall (Phytophthora palmivora) in Thai rubber trees

Author

Promwee, Athakorn, Yenjit, Punnawich, Issarakraisila, Montree, Intana, Warin, and Chamswarng, Chiradej

Abstract

Phytophthoraleaf fall disease in rubber trees is serious and is known to cause a reduction in both the quantity and quality of rubber latex in Thailand. This experiment was conducted to improve the effectiveness of controlling rubber leaf fall disease in indigenous Trichoderma harzianum. The results indicate that the T. harzianumstrain FR-NST-009, isolated from the rhizosphere soil of rubber trees, is more effective at inhibiting the mycelial growth of P. palmivora(66.22%) than the Thai commercial strain T. harzianumCB-Pin-01 (63.51%) with a dual culture technique. This result is clear from the mycoparasitism of the T. harzianumstrain FR-NST-009 under scanning electron microscope. Moreover, the ethyl acetate crude extracts of the T. harzianumstrain FR-NST-009 with a 500 µg ml−1concentration provided the most efficient mycelial inhibition of P. palmivoraby 92.42%. Four bioactive compounds isolated from crude extract and compound 3 provided the highest inhibition of sporangium germination by 98.83%. Compound 3 was then identified as 6-n-pentyl-2H-pyran-2-one using nuclear magnetic resonance spectroscopy. Under field conditions, the T. harzianumstrains of RB-NST-028 and FR-NST-009 were able to control leaf fall disease with no significant difference compared with metalaxyl fungicide after inoculation with P. palmivorafor 120 days.

Published
2017
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