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3. Enhanced differentiation of mesenchymal stem cells for osteochondral constructs

Author

Prosser, Amy

Subjects
616.02
Abstract

Novel osteochondral repair tissue engineering strategies are investigating the use of a single scaffold, with a portion for osteogenic and chondrogenic differentiation, and a single cell source, most notably the mesenchymal stem cell, to facilitate osteochondral differentiation and repair in a single construct. However, this approach requires robust differentiation protocols to ensure that the correct balance of each cell type is produced and maintained. Techniques used to analyse osteogenic and chondrogenic differentiation are well established, but many of the current methods described are qualitative, based on imaging stained cells or sections under a microscope. To facilitate higher throughput screening of chondrogenic differentiation in human MSCs, a novel culture technique using V shaped 96 well plates has been developed combining three robust and quantitative assays. Additionally, two reporter cell lines have been developed that express luciferase under the control of an osteogenic (osteocalcin) or chondrogenic (col2a1) promoter in order to streamline differentiation assays. The use of growth factors to elicit differentiation is well established; with BMP-2 used to enhance osteogenic differentiation and TGF-61 used to enhance chondrogenic differentiation. However, there are several limitations of using growth factors in regenerative medicine and consequently, the use of growth factor mimics was investigated. Two promising growth factor mimics were identified that could support both osteogenic and chondrogenic differentiation; LE135 and imperatorin. LE135, a retinoic acid receptor antagonist, significantly enhanced chondrogenesis with increased GAG production, col2a1 promoter activity and versican mRNA expression and had no significant effect on osteogenic differentiation. Imperatorin, a coumarin derivative, significantly enhanced early stage osteogenesis (alkaline phosphatase activity) and had no significant effect on late stage osteogenesis (mineralisation). Furthermore, chondrogenic differentiation was enhanced by imperatorin with significantly increased GAG production.

Published
2015

5. Data from Targeting the p300/CBP Axis in Lethal Prostate Cancer

Author

Welti, Jonathan, primary, Sharp, Adam, primary, Brooks, Nigel, primary, Yuan, Wei, primary, McNair, Christopher, primary, Chand, Saswati N., primary, Pal, Abhijit, primary, Figueiredo, Ines, primary, Riisnaes, Ruth, primary, Gurel, Bora, primary, Rekowski, Jan, primary, Bogdan, Denisa, primary, West, William, primary, Young, Barbara, primary, Raja, Meera, primary, Prosser, Amy, primary, Lane, Jordan, primary, Thomson, Stuart, primary, Worthington, Jenny, primary, Onions, Stuart, primary, Shannon, Jonathan, primary, Paoletta, Silvia, primary, Brown, Richard, primary, Smyth, Don, primary, Harbottle, Gareth W., primary, Gil, Veronica S., primary, Miranda, Susana, primary, Crespo, Mateus, primary, Ferreira, Ana, primary, Pereira, Rita, primary, Tunariu, Nina, primary, Carreira, Suzanne, primary, Neeb, Antje J., primary, Ning, Jian, primary, Swain, Amanda, primary, Taddei, David, primary, Schiewer, Matthew J., primary, Knudsen, Karen E., primary, Pegg, Neil, primary, and de Bono, Johann S., primary

Published
2023
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6. Supplementary Data from Targeting the p300/CBP Axis in Lethal Prostate Cancer

Author

Welti, Jonathan, primary, Sharp, Adam, primary, Brooks, Nigel, primary, Yuan, Wei, primary, McNair, Christopher, primary, Chand, Saswati N., primary, Pal, Abhijit, primary, Figueiredo, Ines, primary, Riisnaes, Ruth, primary, Gurel, Bora, primary, Rekowski, Jan, primary, Bogdan, Denisa, primary, West, William, primary, Young, Barbara, primary, Raja, Meera, primary, Prosser, Amy, primary, Lane, Jordan, primary, Thomson, Stuart, primary, Worthington, Jenny, primary, Onions, Stuart, primary, Shannon, Jonathan, primary, Paoletta, Silvia, primary, Brown, Richard, primary, Smyth, Don, primary, Harbottle, Gareth W., primary, Gil, Veronica S., primary, Miranda, Susana, primary, Crespo, Mateus, primary, Ferreira, Ana, primary, Pereira, Rita, primary, Tunariu, Nina, primary, Carreira, Suzanne, primary, Neeb, Antje J., primary, Ning, Jian, primary, Swain, Amanda, primary, Taddei, David, primary, Schiewer, Matthew J., primary, Knudsen, Karen E., primary, Pegg, Neil, primary, and de Bono, Johann S., primary

Published
2023
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13. P2.13: Immunosuppression Monotherapy Does Not Protect Donor Leukocytes From Depletion Post MHC-Mismatched Kidney Transplantation in Mice

Author

Dart, Sarah, primary, O’Halloran, Sean, additional, Liu, Liu, additional, Huang, Wen Hua, additional, Kaur, Jaskirat, additional, Zhang, Xiao, additional, Prosser, Amy, additional, Watson, Monalyssa, additional, Lucas, Andrew, additional, Jeffrey, Gary, additional, Joyce, David, additional, and Lucas, Michaela, additional

Published
2022
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18. Dynamic changes to tissue-resident immunity after MHC-matched and MHC-mismatched solid organ transplantation

Author

Prosser, Amy, primary, Huang, Wen Hua, additional, Liu, Liu, additional, Dart, Sarah, additional, Watson, Monalyssa, additional, de Boer, Bastiaan, additional, Kendrew, Philip, additional, Lucas, Andrew, additional, Larma-Cornwall, Irma, additional, Gaudieri, Silvana, additional, Jeffrey, Gary P., additional, Delriviere, Luc, additional, Kallies, Axel, additional, and Lucas, Michaela, additional

Published
2021
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21. Identity and emergency intervention: how social group membership and inclusiveness of group boundaries shape helping behavior

Author

Levine, Mark, Prosser, Amy, Evans, David, and Reicher, Stephen

Subjects
Helping behavior -- Research, Helping behavior -- Psychological aspects, Group identity -- Research, Assistance in emergencies -- Research, Social groups -- Services, Psychology and mental health
Abstract

Two experiments exploring the effects of social category membership on real-life helping behavior are reported. In Study 1, intergroup rivalries between soccer fans are used to examine the role of identity in emergency helping. An injured stranger wearing an in-group team shirt is more likely to be helped than when wearing a rival team shirt or an unbranded sports shirt. In Study 2, a more inclusive social categorization is made salient for potential helpers. Helping is extended to those who were previously identified as out-group members but not to those who do not display signs of group membership. Taken together the studies show the importance of both shared identity between bystander and victim and the inclusiveness of salient identity for increasing the likelihood of emergency intervention. Keywords: social identity; group membership; emergency intervention; helping

Published
2005

24. Solid Organ Transplantation Irrevocably Alters Tissue-Resident Graft Immunity

Author

Prosser, Amy Christine, primary, Huang, Wen Hua, additional, Liu, Liu, additional, Dart, Sarah Jessica, additional, Watson, Monalyssa, additional, de Boer, Bastiaan, additional, Kendrew, Philip John, additional, Lucas, Andrew Douglas, additional, Larma-Cornwall, Irma, additional, Gaudieri, Silvana, additional, Jeffrey, Gary Peter, additional, Delriviere, Luc Denis, additional, Kallies, Axel, additional, and Lucas, Michaela, additional

Published
2020
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29. The educational needs of caregivers of stroke survivors in acute and community settings

Author

O'Connell, Bev, Baker, Linda, and Prosser, Amy

Subjects
Caregivers -- Training, Caregivers -- Research, Stroke (Disease) -- Care and treatment, Stroke (Disease) -- Research
Abstract

Abstract: Stroke is a major cause of chronic illness in Australia, where it is estimated that between 200,000 and 250,000 people live with disabilities due to stroke. Given stroke's effect [...]

Published
2003

34. DC-STAMP knock-down deregulates cytokine production and T-cell stimulatory capacity of LPS-matured dendritic cells

Author

Jansen Bastiaan JH, den Brok Martijn H, Warner Kathrin, Danielski Katharina, Prosser Amy C, Ansems Marleen, Sanecka Anna, Eleveld-Trancikova Dagmar, and Adema Gosse J

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Abstract Background Dendritic cells (DCs) are the highly specialized antigen presenting cells of the immune system that play a key role in regulating immune responses. DCs can efficiently initiate immune responses or induce tolerance. Due to this dual function, DCs are studied in the context of immunotherapy for both cancer and autoimmune diseases. Characterization of DC-specific genes, leading to better understanding of DC immunobiology, will help to guide their use in clinical settings. We previously identified DC-STAMP, a multi-membrane spanning protein preferentially expressed by DCs. DC-STAMP resides in the endoplasmic reticulum (ER) of immature DCs and translocates towards the Golgi compartment upon maturation. In this study we knocked down DC-STAMP in mouse bone marrow-derived DCs (mBMDCs) to determine its function. Results We demonstrate that DC-STAMP knock-down mBMDCs secrete less IL-6, IL-12, TNF-α and IL-10 while IL-1 production is enhanced. Moreover, LPS-matured DC-STAMP knock-down mBMDCs show impaired T cell activation potential and induction of Th1 responses in an alloreaction. Conclusions We show that DC-STAMP plays an important role in cytokine production by mBMDCs following LPS exposure. Our results reveal a novel function of DC-STAMP in regulating DC-initiated immune responses.

Published
2011
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37. Tech Out Your Classroom : 6 Projects to Meet Common Core & ISTE Standards

Author

Prosser, Amy and Prosser, Amy

Subjects
Common Core State Standards (Education), Educational technology--Study and teaching--Activity programs, Project method in teaching
Abstract

This book walks the educator through six technology-rich, customizable projects aligned to Common Core and ISTE Standards. Beginning with a look at the objectives, the author then presents subject-specific recommendations for tailoring the project to fit English, math, science, social studies, foreign language or art classrooms. A discussion of applicable technology tools accompanies each project, followed by step-by-step guidance, grading considerations, examples and tips on troubleshooting.

Published
2016

38. Evidence of CD4+ T cell-mediated immune pressure on the Hepatitis C virus genome

Author

Lucas, Michaela, primary, Deshpande, Pooja, additional, James, Ian, additional, Rauch, Andri, additional, Pfafferott, Katja, additional, Gaylard, Elouise, additional, Merani, Shahzma, additional, Plauzolles, Anne, additional, Lucas, Andrew, additional, McDonnell, Wyatt, additional, Kalams, Spyros, additional, Pilkinton, Mark, additional, Chastain, Cody, additional, Barnett, Louise, additional, Prosser, Amy, additional, Mallal, Simon, additional, Fitzmaurice, Karen, additional, Drummer, Heidi, additional, Ansari, M. Azim, additional, Pedergnana, Vincent, additional, Barnes, Ellie, additional, John, Mina, additional, Kelleher, Dermot, additional, Klenerman, Paul, additional, and Gaudieri, Silvana, additional

Published
2018
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40. In vitro degradation and cytocompatibility of PLA-PCL copolymer unit cell scaffolds

Author

Scotchford Colin, Prosser Amy, Sottile Virginie, Ahmed Ifty, Grant David, Felfel Reda, and Gimeno-Fabra Miquel

Subjects
0301 basic medicine, Histology, Chemistry, Cell, Biomedical Engineering, Bioengineering, 02 engineering and technology, 021001 nanoscience & nanotechnology, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Chemical engineering, medicine, Copolymer, In vitro degradation, 0210 nano-technology, Biotechnology
Published
2016

41. Abstract 1575: Novel small molecule inhibitors of p300/CBP down-regulate AR and c-Myc for the treatment of castrate resistant prostate cancer

Author

Brooks, Nigel, primary, Pegg, Neil, additional, Worthington, Jenny, additional, Young, Barbara, additional, Prosser, Amy, additional, Lane, Jordan, additional, Taddei, David, additional, Schiewer, Matthew, additional, deLeeuw, Renee, additional, McCann, Jennifer, additional, and Knusden, Karen, additional

Published
2017
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42. Characterisation of CCS1477: A novel small molecule inhibitor of p300/CBP for the treatment of castration resistant prostate cancer.

Author

Pegg, Neil, primary, Brooks, Nigel, additional, Worthington, Jenny, additional, Young, Barbara, additional, Prosser, Amy, additional, Lane, Jordan, additional, Taddei, David, additional, Brown, Richard, additional, Harbottle, Gareth, additional, Shannon, Jonathan, additional, Paoletta, Silvia, additional, and Knudsen, Karen E., additional

Published
2017
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44. Enterovirus Exposure Uniquely Discriminates Type 1 Diabetes Patients with a Homozygous from a Heterozygous Melanoma Differentiation-Associated Protein 5/Interferon Induced with Helicase C Domain 1A946T Genotype

Author

Schulte, Barbara M., primary, Gielen, Paul R., additional, Kers-Rebel, Esther D., additional, Prosser, Amy C., additional, Lind, Katharina, additional, Flodström-Tullberg, Malin, additional, Tack, Cees J., additional, Elving, Lammy D., additional, and Adema, Gosse J., additional

Published
2016
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