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25 results on '"Puig-Basagoiti, Francesc"'

3. Prevalence and route of transmission of infection with a novel DNA virus (TTV), hepatitis C virus, and hepatitis G virus in patients infected with HIV

Author

Puig-Basagoiti, Francesc, Cabana, Marta, Guilera, Magda, Gimenez-Barcons, Mireia, Sirera, Guillem, Tural, Cristina, Clotet, Bonaventura, Sanchez-Tapias, Jose-Maria, Rodes, Juan, Saiz, Juan-Carlos, and Martinez, Miguel-Angel

Subjects
TT virus, HIV patients -- Diseases, Health
Abstract

The TT virus appears to be transmitted sexually and through blood transfusion. This newly discovered virus does not appear to adversely affect HIV patients.

Published
2000

5. Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation

Author

Puig-Basagoiti, Francesc, primary, Fukuhara, Takasuke, additional, Tamura, Tomokazu, additional, Ono, Chikako, additional, Uemura, Kentaro, additional, Kawachi, Yukako, additional, Yamamoto, Satomi, additional, Mori, Hiroyuki, additional, Kurihara, Takeshi, additional, Okamoto, Toru, additional, Aizaki, Hideki, additional, and Matsuura, Yoshiharu, additional

Published
2016
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6. Valor pronostico de la heterogeneidad genética de la proteína NS5A del virus de la hepatitis C (VHC) en la terapia combinada con interferón (IFN) y ribavirina (Rbv)

Author

Puig-Basagoiti, Francesc, Forns, Xavi, Furčić, Ivana, Ampurdanés, Sergi, Giménez-Barcons, Mireia, Rodés, Juan, Sánchez-Tapias Jose-Maria, and Saiz, Juan Carlos

Subjects
HCV, quasispecies, NS5A, terapia combinada IFN/Rbv
Abstract

Valor pronostico de la heterogeneidad genética de la proteína NS5A del virus de la hepatitis C (VHC) en la terapia combinada con interferón (IFN) y ribavirina (Rbv)

Published
2003

7. Characterization and Evolution of NS5A-HCV Quasispecies during Interferon plus Ribavirin (IFN+RIB) Therapy: the V3 Region as Predictor of the Type of Response

Author

Puig-Basagoiti, Francesc, Ampurdanes, Sergi, Furčić, Ivana, Forns, Xavi, Giménez-Barcons, Mireia, Rodes, Juan, Sanchez-Tapias, Jose-Maria, and Saiz, Juan Carlos

Subjects
viruses, virus diseases, HCV, quasispecies, V3, IFN+RIB therapy, biochemical phenomena, metabolism, and nutrition, digestive system diseases
Abstract

Characterization and Evolution of NS5A-HCV Quasispecies during Interferon plus Ribavirin (IFN+RIB) Therapy: the V3 Region as Predictor of the Type of Response

Published
2002

8. Combination Therapy in Genotype 1 Chronic Hepatitis C. Effectiveness of Induction with Interferon and Early Prediction of Response

Author

Ampurdanes, Sergi, Puig-Basagoiti, Francesc, Forns, Xavi, Saiz, Juan Carlos, Costa, Josep, Furčić, Ivana, Martinez-Bauer, Eva, Sanchez-Tapias, Jose-Maria, and Rodes, Juan

Subjects
HCV, genotype 1, chronic hepatitis, IFN
Abstract

Combination Therapy in Genotype 1 Chronic Hepatitis C. Effectiveness of Induction with Interferon and Early Prediction of Response

Published
2002

14. Triaryl Pyrazoline Compound Inhibits Flavivirus RNA Replication

Author

Puig-Basagoiti, Francesc, primary, Tilgner, Mark, additional, Forshey, Brett M., additional, Philpott, Sean M., additional, Espina, Noel G., additional, Wentworth, David E., additional, Goebel, Scott J., additional, Masters, Paul S., additional, Falgout, Barry, additional, Ren, Ping, additional, Ferguson, David M., additional, and Shi, Pei-Yong, additional

Published
2006
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21. Influence of the genetic heterogeneity of the ISDR and PePHD regions of hepatitis C virus on the response to interferon therapy in chronic hepatitis C

Author

Puig-Basagoiti, Francesc, primary, Sáiz, Juan-Carlos, additional, Forns, Xavier, additional, Ampurdanès, Sergi, additional, Giménez-Barcons, Mireia, additional, Franco, Sandra, additional, Sánchez-Fueyo, Alberto, additional, Costa, Josep, additional, Sánchez-Tapias, José-María, additional, and Rodés, Juan, additional

Published
2001
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23. Identification of Compounds with Anti-West Nile Virus Activity

Author

R. Goodell, John, Puig-Basagoiti, Francesc, M. Forshey, Brett, Shi, Pei-Yong, and M. Ferguson, David

Abstract

The lack of antiviral compounds targeting flaviviruses represents a significant problem in the development of strategies for treating West Nile Virus (WNV), Dengue, and Yellow Fever infections. Using WNV high-throughput screening techniques developed in our laboratories, we report the identification of several small molecule anti-WNV compounds belonging to four different structural classes including pyrazolines, xanthanes, acridines, and quinolines. The initial set of “hits” was further refined using cell viability-cytotoxicity assays to two 1,3,5-triaryl pyrazoline compounds:  1-(4-chlorophenylacetyl)-5-(4-nitrophenyl)-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazole and 1-benzoyl-5-(4-chlorophenyl)-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazole. On the basis of their activity and favorable therapeutic indexes, these compounds were identified as viable leads and subjected to additional evaluation using an authentic viral titer reduction assay employing an epidemic strain of WNV. The compounds were further evaluated in a transient replicon reporting system to gain insight into the mechanism of action by identifying the step at which inhibition takes place during viral replication. The results indicate the pyrazolines inhibit RNA synthesis, pointing to viral RNA polymerase, RNA helicase, or other viral replication enzymes as potential targets. Progress was also made in understanding the structural requirements for activity by synthesizing a focused chemical library of substituted pyrazolines. Preliminary SAR data are presented that show the aryl-rings are required for activity against WNV. More importantly, the results indicate WNV activity is tolerant to aryl-substitutions paving the way for the design and development of much larger combinatorial libraries with varied physicochemical properties.

Published
2006
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24. West Nile Virus Methyltransferase Catalyzes Two Methylations of the Viral RNA Cap through a Substrate-Repositioning Mechanism.

Author

Hongping Dong, Ren, Suping, Bo Zhang, Yangsheng Zhou, Puig-Basagoiti, Francesc, Hongmin Li, and Pei-Yong Shi

Subjects
*FLAVIVIRUSES, *METHYLTRANSFERASES, *METHYLATION, *BINDING sites, *RNA
Abstract

Flaviviruses encode a single methyltransferase domain that sequentially catalyzes two methylations of the viral RNA cap, GpppA-RNA → m7GpppA-RNA → m7GpppAm-RNA, by using S-adenosyl-L-methionine (SAM) as a methyl donor. Crystal structures of flavivirus methyltransferases exhibit distinct binding sites for SAM, GTP, and RNA molecules. Biochemical analysis of West Nile virus methyltransferase shows that the single SAM-binding site donates methyl groups to both N7 and 2'-O positions of the viral RNA cap, the GTP-binding pocket functions only during the 2'-O methylation, and two distinct sets of amino acids in the RNA-binding site are required for the N7 and 2'-O methylations. These results demonstrate that flavivirus methyltransferase catalyzes two cap methylations through a substrate-repositioning mechanism. In this mechanism, guanine N7 of substrate GpppA-RNA is first positioned to SAM to generate m7GpppA-RNA, after which the m7G moiety is repositioned to the GTP-binding pocket to register the 2'-OH of the adenosine with SAM, generating m7GpppAm-RNA. Because N7 cap methylation is essential for viral replication, inhibitors designed to block the pocket identified for the N7 cap methylation could be developed for flavivirus therapy. [ABSTRACT FROM AUTHOR]

Published
2008
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25. Distinct RNA Elements Confer Specificity to Flavivirus RNA Cap Methylation Events.

Author

Hongping Dong, Ray, Debashish, Suping Ren, Bo Zhang, Puig-Basagoiti, Francesc, Takagi, Yuko, Kiong Ho, C., Hongmin Li, and Pei-Yong Shi

Subjects
*FLAVIVIRUSES, *METHYLATION, *RNA viruses, *GENOMES, *NUCLEOTIDES, *WEST Nile virus, *METHYLTRANSFERASES
Abstract

The 5′ end of the flavivirus plus-sense RNA genome contains a type 1 cap (m7 GpppAmG), followed by a conserved stem-loop structure. We report that nonstructural protein 5 (NS5) from four serocomplexes of flaviviruses specifically methylates the cap through recognition of the 5′ terminus of viral RNA. Distinct RNA elements are required for the methylations at guanine N-7 on the cap and ribose 2′-OH on the first transcribed nucleotide. In a West Nile virus (WNV) model, N-7 cap methylation requires specific nucleotides at the second and third positions and a 5′ stem-loop structure; in contrast, 2′-OH ribose methylation requires specific nucleotides at the first and second positions, with a minimum 5′ viral RNA of 20 nucleotides. The cap analogues GpppA and m7 GpppA are not active substrates for WNV methytransferase. Footprinting experiments using Gppp- and m7 Gppp-terminated RNAs suggest that the 5′ termini of RNA substrates interact with NS5 during the sequential methylation reactions. Cap methylations could be inhibited by an antisense oligomer targeting the first 20 nucleotides of WNV genome. The viral RNA-specific cap methylation suggests methyltransferase as a novel target for flavivirus drug discovery. [ABSTRACT FROM AUTHOR]

Published
2007
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