Rice (O. sativa L.) is one among the necessary food crops worldwide. Due to ever-increasing demand, many are undertaking several efforts to enhance its productivity - the latest being the sequel of Rice genome sequencing projects. The accelerated developments in next-generation sequencing (NGS) has bolstered these efforts in hundreds to thousands of rice varieties, which has enabled researchers to unpack the hidden potential of vast and diverse rice germplasm. One of the important objectives of these projects is to accurately characterize the gene models, which has a major significance for the in-depth study of gene function and, thus, various applications. Bioinformatics plays a major role in gene structure identification and its biological function through various algorithms and software. Hence, this chapter aims to elucidate the approach of identifying, characterizing, and finding the function of different types of rice genes.