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9. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis

Author

Luo, Zhao-Bo, primary, Han, Shengzhong, primary, Yin, Xi-Jun, primary, Liu, Hongye, additional, Wang, Junxia, additional, Xuan, Meifu, additional, Hao, Chunyun, additional, Wang, Danqi, additional, Liu, Yize, additional, Chang, Shuangyan, additional, Li, Dongxu, additional, Gao, Kai, additional, Li, Huiling, additional, Quan, Biaohu, additional, Quan, Lin-Hu, additional, and Kang, Jin-Dan, additional

Published
2023
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10. Dynamic changes in intestinal microbiota and metabolite composition of pre-weaned beef calves

Author

Liu, Hongye, primary, Yan, Chunri, additional, Hao, Chunyun, additional, Wang, Danqi, additional, Liu, Yize, additional, Luo, Zhao-Bo, additional, Han, Sheng-Zhong, additional, Wang, Jun-Xia, additional, Li, Dongxu, additional, Zhu, Jun, additional, Chang, Shuang-Yan, additional, Yang, Liu-Hui, additional, Lin, Xuemei, additional, Yan, Changguo, additional, Kang, Jin-Dan, additional, and Quan, Lin-Hu, additional

Published
2023
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11. Ginsenoside Rg1 activates brown adipose tissue to counteract obesity in high-fat diet-fed mice by regulating gut microbes and bile acid composition

Author

Liu, Yize, primary, Jin, Zheng-Yun, additional, Wang, Jun-Xia, additional, Wang, Danqi, additional, Liu, Hongye, additional, Li, Dongxu, additional, Zhu, Jun, additional, Luo, Zhao-Bo, additional, Han, Sheng-Zhong, additional, Chang, Shuang-Yan, additional, Yang, Liu-Hui, additional, Kang, Jin-Dan, additional, and Quan, Lin-Hu, additional

Published
2023
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12. Author response: Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis

Author

Luo, Zhao-Bo, primary, Han, Shengzhong, primary, Yin, Xi-Jun, primary, Liu, Hongye, additional, Wang, Junxia, additional, Xuan, Meifu, additional, Hao, Chunyun, additional, Wang, Danqi, additional, Liu, Yize, additional, Chang, Shuangyan, additional, Li, Dongxu, additional, Gao, Kai, additional, Li, Huiling, additional, Quan, Biaohu, additional, Quan, Lin-Hu, additional, and Kang, Jin-Dan, additional

Published
2022
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13. Gastrodia elata Blume extract improves high-fat diet-induced type 2 diabetes by regulating gut microbiota and bile acid profile

Author

Wang, Danqi, primary, Wang, Jun-Xia, additional, Yan, Chunri, additional, Liu, Yize, additional, Liu, Hongye, additional, Li, Dongxu, additional, Zhu, Jun, additional, Luo, Zhao-Bo, additional, Han, Sheng-Zhong, additional, Jin, Zheng-Yun, additional, Chang, Shuang-Yan, additional, Yang, Liu-Hui, additional, Kang, Jin-Dan, additional, and Quan, Lin-Hu, additional

Published
2022
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15. Brown adipose tissue activation with ginsenoside compound K ameliorates polycystic ovary syndrome.

Author

Ye, Rongcai, Yan, Chunlong, Zhou, Huiqiao, Zhang, Chuanhai, Huang, Yuanyuan, Dong, Meng, Zhang, Hanlin, Lin, Jun, Jiang, Xiaoxiao, Yuan, Shouli, Chen, Li, Jiang, Rui, Cheng, Ziyu, Zheng, Kexin, Yu, Anni, Zhang, Qiaoli, Quan, Lin‐hu, Jin, Wanzhu, and Quan, Lin-Hu

Abstract

Background and Purpose: Polycystic ovary syndrome (PCOS) is a common metabolic and endocrine disease affecting women of reproductive age. Due to its complex aetiology, there is no currently effective cure for PCOS. Brown adipose tissue (BAT) activity is significantly decreased in PCOS patients, and BAT activation has beneficial effects in animal models of PCOS. Here, we investigated the effect of ginsenoside compound K (CK) in an animal model of PCOS and its mechanism of BAT activation.Experimental Approach: Primary brown adipocytes, Db/Db mice and dehydroepiandrosterone (DHEA)-induced PCOS rats were used. The core body temperature, oxygen consumption, energy metabolism related gene and protein expression were assessed to identify the effect of CK on overall energy metabolism. Oestrous cycle, serum sex hormone, ovarian steroidogenic enzyme gene expression and ovarian morphology were also evaluated following CK treatment.Key Results: Our results indicated that CK treatment could significantly protect against body weight gain in Db/Db mice via BAT activation. Furthermore, we found that CK treatment could normalize hyperandrogenism, oestrous cyclicity, normalize steroidogenic enzyme expression and decrease the number of cystic follicles in PCOS rats. Interestingly, as a potential endocrine intermediate, C-X-C motif chemokine ligand-14 protein (CXCL14) was significantly up-regulated following CK administration. In addition, exogenous CXC14 supplementation was found to reverse DHEA-induced PCOS in a phenotypically similar manner to CK treatment.Conclusion and Implications: In summary, CK treatment significantly activates BAT, increases CXCL14 expression and ameliorates PCOS. These findings suggest that CK might be a potential drug candidate for PCOS treatment. [ABSTRACT FROM AUTHOR]

Published
2022
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17. Myostatin gene deletion alters gut microbiota stimulating fast-twitch glycolytic muscle growth

Author

Luo, Zhao-Bo, primary, Han, Shengzhong, additional, Yin, Xi-Jun, additional, Liu, Hongye, additional, Wang, Junxia, additional, Xuan, Meifu, additional, Hao, Chunyun, additional, Wang, Danqi, additional, Liu, Yize, additional, Chang, Shuangyan, additional, Li, Dongxu, additional, Gao, Kai, additional, Li, Huiling, additional, Quan, Biaohu, additional, Quan, Lin-Hu, additional, and Kang, Jin-Dan, additional

Published
2022
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19. Dynamic Changes of Intestinal Microbiota and Metabolite Composition in Pre-Weaned Beef Calves

Author

Liu, Hongye, primary, Yan, Chunri, additional, Hao, Chunyun, additional, Wang, Danqi, additional, Liu, Yize, additional, Luo, Zhao-Bo, additional, Han, Shengzhong, additional, Wang, Junxia, additional, Li, Dongxu, additional, Zhu, Jun, additional, Chang, Shuangyan, additional, Yang, Liuhui, additional, Lin, Xuemei, additional, Yan, Changguo, additional, Kang, Jin-Dan, additional, and Quan, Lin-Hu, additional

Published
2021
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27. miR-455-3p Is Negatively Regulated by Myostatinin Skeletal Muscle and Promotes Myoblast Differentiation

Author

Han, Sheng-Zhong, Gao, Kai, Chang, Shuang-Yan, Choe, Hak-Myong, Paek, Hyo-Jin, Quan, Biao-Hu, Liu, Xin-Yue, Yang, Liu-Hui, Lv, Si-Tong, Yin, Xi-Jun, Quan, Lin-Hu, and Kang, Jin-Dan

Abstract

Myostatin (MSTN) is a growth and differentiation factor that regulates proliferation and differentiation of myoblasts, which in turn controls skeletal muscle growth. It may regulate myoblast differentiation by influencing miRNA expression, and the present study aimed to clarify its precise mechanism of action. Here, we found that MSTN–/–pigs showed an overgrowth of skeletal muscle and upregulated miR-455-3p level. Intervention of MSTNexpression using siMSTN in C2C12 myoblasts also showed that siMSTN significantly increased the expression of miR-455-3p. It was found that miR-455-3p directly targeted the 3′-untranslated region of Smad2by dual-luciferase assay. qRT-PCR, Western blotting, and immunofluorescence analyses indicated that miR-455-3p overexpression or Smad2silencing in C2C12 myoblasts significantly promoted myoblast differentiation. Furthermore, siMSTN significantly increased the expression of GATA3. The levels of miR-455-3p were considerably reduced in C2C12 myoblasts following GATA3knockdown. Consistently, GATA3knockdown also reduced the enhanced miR-455-3p expression caused by siMSTN. Finally, we illustrated that GATA3has a role in myoblast differentiation regulation. Taken together, we identified the expression profiles of miRNAs in MSTN–/–pigs and found that miR-455-3p positively regulates myoblast differentiation. In addition, we revealed that MSTN acts through the GATA3/miR-455-3p/Smad2 cascade to regulate muscle development.

Published
2022
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28. Myristoleic acid produced by enterococci reduces obesity through brown adipose tissue activation

Author

Quan, Lin-Hu, primary, Zhang, Chuanhai, additional, Dong, Meng, additional, Jiang, Jun, additional, Xu, Hongde, additional, Yan, Chunlong, additional, Liu, Xiaomeng, additional, Zhou, Huiqiao, additional, Zhang, Hanlin, additional, Chen, Li, additional, Zhong, Fei-Liang, additional, Luo, Zhao-Bo, additional, Lam, Sin-Man, additional, Shui, Guanghou, additional, Li, Donghao, additional, and Jin, Wanzhu, additional

Published
2019
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34. Myristoleic acid produced by enterococci reduces obesity through brown adipose tissue activation

Author

Quan, Lin-Hu, Zhang, Chuanhai, Dong, Meng, Jiang, Jun, Xu, Hongde, Yan, Chunlong, Liu, Xiaomeng, Zhou, Huiqiao, Zhang, Hanlin, Chen, Li, Zhong, Fei-Liang, Luo, Zhao-Bo, Lam, Sin-Man, Shui, Guanghou, Li, Donghao, and Jin, Wanzhu

Abstract

ObjectiveDietary fibre has beneficial effects on energy metabolism, and the majority of studies have focused on short-chain fatty acids produced by gut microbiota. Ginseng has been reported to aid in body weight management, however, its mechanism of action is not yet clear. In this study, we focused on the potential modulating effect of ginseng on gut microbiota, aiming to identify specific strains and their metabolites, especially long-chain fatty acids (LCFA), which mediate the anti-obesity effects of ginseng.DesignDb/db mice were gavaged with ginseng extract (GE) and the effects of GE on gut microbiota were evaluated using 16S rDNA-based high throughput sequencing. To confirm the candidate fatty acids, untargeted metabolomics analyses of the serum and medium samples were performed.ResultsWe demonstrated that GE can induce Enterococcus faecalis, which can produce an unsaturated LCFA, myristoleic acid (MA). Our results indicate that E. faecalisand its metabolite MA can reduce adiposity by brown adipose tissue (BAT) activation and beige fat formation. In addition, the gene of E. faecalisencoding Acyl-CoA thioesterases (ACOTs) exhibited the biosynthetic potential to synthesise MA, as knockdown (KD) of the ACOT gene by CRISPR-dCas9 significantly reduced MA production. Furthermore, exogenous treatment with KD E. faecaliscould not reproduce the beneficial effects of wild type E. faecalis, which work by augmenting the circulating MA levels.ConclusionsOur results demonstrated that the gut microbiota-LCFA-BAT axis plays an important role in host metabolism, which may provide a strategic advantage for the next generation of anti-obesity drug development.

Published
2020
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35. Enzymatic Biotransformation of Ginsenoside Rb1 to Compound K by Recombinant β-Glucosidase from Microbacterium esteraromaticum

Author

Quan, Lin-Hu, Min, Jin-Woo, Jin, Yan, Wang, Chao, Kim, Yeon-Ju, and Yang, Deok-Chun

Abstract

We cloned and characterized a β-glucosidase (bgp3) gene from Microbacterium esteraromaticumisolated from ginseng field. The bgp3gene consists of 2,271 bp encoding 756 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. The molecular mass of purified Bgp3 was 80 kDa, as determined by SDS–PAGE. The enzyme (Bgp3) catalyzed the conversion of ginsenoside Rb1 to the more pharmacologically active minor ginsenoside Rd and compound K. The Bgp3 hydrolyzed the outer glucose moiety attached to the C-20 position of ginsenoside Rb1, followed by hydrolysis of the inner glucose moiety attached to the C-3 position. Using 0.1 mg mL–1enzyme in 20 mM sodium phosphate buffer at 40 °C and pH 7.0, 1.0 mg mL–1ginsenoside Rb1 was transformed into 0.46 mg mL–1compound K within 60 min with a corresponding molar conversion yield of 77%. Bgp3 hydrolyzed the ginsenoside Rb1 along the following pathway: Rb1 → Rd → compound K.

Published
2024
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37. Metabolite profiling of ginsenosides in rat plasma, urine and feces by LC–MS/MS and its application to a pharmacokinetic study after oral administration of Panax ginseng extract.

Author

Dong, Wei‐Wei, Han, Xiong‐Zhe, Zhao, Jinhua, Zhong, Fei‐Liang, Ma, Rui, Wu, Songquan, Li, Donghao, Quan, Lin‐Hu, and Jiang, Jun

Abstract

Abstract: Panax ginseng is widely consumed as a functional food in the form of tea, powder, capsules, among others, and possesses a range of pharmacological activities including adaptogenic, immune‐modulatory, anti‐tumor, anti‐aging and anti‐inflammatory effects. The aim of this study was to identify and quantify the major ginsenosides and their metabolites in rat plasma, urine and feces after administration of P. ginseng extract using LC–MS/MS. We collected rat plasma samples at 0.5, 1, 2, 4, 8, 12, 24 and 48 h, and the amounts of urine and fecal samples accumulated in 24 h. Fourteen major ginsenosides and their metabolites were observed in fecal samples at high levels; however, low levels of 11 ginsenosides were detected in urine samples. The pharmacokinetics of the major ginsenosides and their metabolites was investigated in plasma. The results indicated that the maximum plasma concentration, time to maximum concentration and area under the curve of compound K were significantly greater than those of other ginsenosides. This study thus provides valuable information for drug development and clinical application of P. ginseng. [ABSTRACT FROM AUTHOR]

Published
2018
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38. Purification and Characterization of Pasteuricin Produced by Staphylococcus pasteuriRSP-1 and Active against Multidrug-Resistant Staphylococcus aureus

Author

Hong, Jisoo, Kim, Jonguk, Quan, Lin-Hu, Heu, Sunggi, and Roh, Eunjung

Abstract

Staphylococcus aureusis an important pathogen implicated in various diseases, including staphylococcal food poisoning. Bacteriocins are considered safe and effective antimicrobial substances for the prevention of the growth of pathogenic bacteria. In this article, we describe the purification and characterization of pasteuricin, a novel bacteriocin produced by Staphylococcus pasteuriRSP-1. A cell-free supernatant of S. pasteuriRSP-1 exerted strong antimicrobial activity against staphylococci, including methicillin-resistant S. aureus(MRSA), and gram-positive bacteria. The loss of antimicrobial activity upon treatment with proteolytic enzymes confirmed the proteinaceous nature of pasteuricin. A rapid and pronounced bactericidal effect of pasteuricin was confirmed by a live-dead bacterial viability assay. To our knowledge, pasteuricin is the first reported S. pasteuribacteriocin that inhibits S. aureus. Because pasteuricin is characterized by strong antimicrobial activity and high stability, it has potential as an alternative antimicrobial agent to antibiotics.

Published
2018
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39. Bioconversion of ginsenoside Rb1 into compound K by Leuconostoc citreum LH1 isolated from kimchi

Author

Quan, Lin-Hu, Piao, Jin-Ying, Min, Jin-Woo, Yang, Dong-Uk, Lee, Hee Nyeong, and Yang, Deok Chun

Subjects
bioconversion, ginsenoside Rb1, Food Microbiology, lcsh:QR1-502, compound K, Leuconostoc citreum LH1, Microbiology, lcsh:Microbiology, eye diseases, Research Paper
Abstract

About 40 different types of ginsenoside (ginseng saponin), a major pharmacological component of ginseng, have been identified along with their physiological activities. Among these, compound K has been reported to prevent the development of and the metastasis of cancer by blocking the formation of tumors and suppressing the invasion of cancerous cells. In this study, ginsenoside Rb1 was converted into compound K via interaction with the enzyme secreted by β-glucosidase active bacteria, Leuconostoc citreum LH1, extracted from kimchi. The optimum time for the conversion of Rb1 to compound K was about 72 hrs at a constant pH of 6.0 and an optimum temperature of about 30ºC. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 hrs post-reaction (99%). Both TLC and HPLC were used to analyze the enzymatic reaction. Ginsenoside Rb1 was consecutively converted to ginsenoside Rd, F2, and compound K via the hydrolyses of 20-C β-(1 → 6)-glucoside, 3-C β-(1 → 2)glucoside, and 3-C β-glucose of ginsenoside Rb1.

Published
2011

42. Biotransformation of Panax ginsengextract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry

Author

Dong, Wei-Wei, Zhao, Jinhua, Zhong, Fei-Liang, Zhu, Wen-Jing, Jiang, Jun, Wu, Songquan, Yang, Deok-Chun, Li, Donghao, and Quan, Lin-Hu

Abstract

In general, after Panax ginsengis administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginsengby microflora are important for obtaining a better understanding of their biological effects.

Published
2017
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48. Isolation and characterization of novel ginsenoside-hydrolyzing glycosidase from Microbacterium esteraromaticum that transforms ginsenoside Rb2 to rare ginsenoside 20( S)-Rg3.

Author

Quan, Lin-Hu, Wang, Chao, Jin, Yan, Wang, Ting-Rui, Kim, Yeon-Ju, and Yang, Deok

Abstract

Ginsenoside Rb2 was transformed by recombinant glycosidase (Bgp2) into ginsenosides Rd and 20( S)-Rg3. The bgp2 gene consists of 2,430 bp that encode 809 amino acids, and this gene has homology to the glycosyl hydrolase family 2 protein domain. SDS-PAGE was used to determine that the molecular mass of purified Bgp2 was 87 kDa. Using 0.1 mg ml of enzyme in 20 mM sodium phosphate buffer at 40 °C and pH 7.0, 1.0 mg ml ginsenoside Rb2 was transformed into 0.47 mg ml ginsenoside 20( S)-Rg3 within 120 min, with a corresponding molar conversion yield of 65 %. Bgp2 hydrolyzed the ginsenoside Rb2 along the following pathway: Rb2 → Rd → 20( S)-Rg3. This is the first report of the biotransformation of ginsenoside Rb2 to ginsenoside 20( S)-Rg3 using the recombinant glycosidase. [ABSTRACT FROM AUTHOR]

Published
2013
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49. Enzymatic biotransformation of ginsenoside Rb1 to 20( S)-Rg3 by recombinant β-glucosidase from Microbacterium esteraromaticum.

Author

Quan, Lin-Hu, Min, Jin-Woo, Yang, Dong-Uk, Kim, Yeon-Ju, and Yang, Deok-Chun

Subjects
BIOTRANSFORMATION (Metabolism), GINSENOSIDES, GLUCOSIDASES, MICROBACTERIUM, ESCHERICHIA coli
Abstract

Microbacterium esteraromaticum was isolated from ginseng field. The β-glucosidase gene ( bgp1) from M. esteraromaticum was cloned and expressed in Escherichia coli BL21 (DE3). The bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. The recombinant β-glucosidase enzyme (Bgp1) was purified and characterized. The molecular mass of purified Bgp1 was 87.5 kDa, as determined by SDS-PAGE. Using 0.1 mg ml enzyme in 20 mM sodium phosphate buffer at 37°C and pH 7.0, 1.0 mg ml ginsenoside Rb1 was transformed into 0.444 mg ml ginsenoside Rg3 within 6 h. The Bgp1 sequentially hydrolyzed the outer and inner glucose attached to the C-20 position of ginsenosides Rb1. Bgp1 hydrolyzed the ginsenoside Rb1 along the following pathway: Rb1 → Rd → 20( S)-Rg3. This is the first report of the biotransformation of ginsenoside Rb1 to ginsenoside 20( S)-Rg3 using the recombinant β-glucosidase. [ABSTRACT FROM AUTHOR]

Published
2012
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50. Biotransformation of Ginsenoside Rb1 to Prosapogenins, Gypenoside XVII, Ginsenoside Rd, Ginsenoside F2, and Compound K by Leuconostoc mesenteroides DC102.

Author

Quan LH, Piao JY, Min JW, Kim HB, Kim SR, Yang DU, and Yang DC

Abstract

Ginsenoside Rb1is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside Rb1 was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside F2 and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about 30℃. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by highperformance liquid chromatography, suggesting the transformation pathway: ginsenoside Rb1→ gypenoside XVII and ginsenoside Rd→ginsenoside F2→compound K.

Published
2011
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