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38 results on '"Qi, Xiaoyi"'

11. Association between schizophrenia and psoriasis: A two‐sample bidirectional Mendelian randomization study.

Author

Ou, Yanting, Qi, Xiaoyi, Zhang, Yihan, Liu, Dandan, Luo, Lin, Wu, Mengfan, Xu, Xiangwen, and Cui, Yongyan

Subjects
*SCHIZOPHRENIA, *PSORIASIS, *GENOME-wide association studies
Abstract

Background: Observational studies have showed an association between schizophrenia and risk of psoriasis and vice versa. However, whether schizophrenia is causally associated with psoriasis is unclear. Method s : A two‐sample bidirectional Mendelian randomization (MR) analysis was performed with publicly available genome‐wide association study data including schizophrenia (n = 77 096) and psoriasis (n = 462 933). The inverse‐variance weighted method was performed as the main analysis, with a complementary with the other two analyses: MR‐Egger and weighted median method. A series of sensitivity analyses were also conducted to evaluate the robustness of the results. Results: MR analyses indicated that genetically predicted schizophrenia was significantly associated with an increased risk of psoriasis [OR: 1.001, 95% confidence interval (CI): 1.000–1.002, p = 0.012]. However, no causal effect of genetically predicted psoriasis on schizophrenia (OR: 0.221, 95% CI: 0.029–1.682, p = 0.145) was detected. No pleiotropy or heterogeneity was detected in sensitivity analysis (all p > 0.05). Conclusions: Our study provides genetic evidence for the causal association between schizophrenia and psoriasis. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Sanguinarine inhibits melanoma invasion and migration by targeting the FAK/PI3K/AKT/mTOR signalling pathway.

Author

Qi, Xiaoyi, Chen, Yonglan, Liu, Sha, Liu, Li, Yu, Zehui, Yin, Ling, Fu, Lu, Deng, Mingming, Liang, Sicheng, and Lü, Muhan

Subjects
*CELLULAR signal transduction, *SANGUINARINE, *MTOR protein, *MELANOMA, *MOLECULAR docking
Abstract

Sanguinarine (SAG) is the most abundant constituent of Macleaya cordata (Willd.) R. Br. (Popaceae). SAG has shown antimammary and colorectal metastatic effects in mice in vivo, suggesting its potential for cancer chemotherapy. To determine the antimetastatic effect and underlying molecular mechanisms of SAG on melanoma. CCK8 assay was used to determine the inhibition of SAG on the proliferation of A375 and A2058 cells. Network pharmacology analysis was applied to construct a compound-target network and select potential therapeutic targets of SAG against melanoma. Molecular docking simulation was conducted for further analysis of the selected targets. In vitro migration/invasion/western blot assay with 1, 1.5, 2 μM SAG and in vivo effect of 2, 4, 8 mg/kg SAG in xenotransplantation model in nude mice. The key targets of SAG treatment for melanoma were mainly enriched in PI3K-AKT pathway, and the binding energy of SAG to PI3K, AKT, and mTOR were −6.33, −6.31, and −6.07 kcal/mol, respectively. SAG treatment inhibited the proliferation, migration, and invasion ability of A375 and A2058 cells (p < 0.05) with IC50 values of 2.378 μM and 2.719 μM, respectively. It also decreased the phosphorylation levels of FAK, PI3K, AKT, mTOR and protein expression levels of MMP2 and ICAM-2. In the nude mouse xenograft model, 2, 4, 8 mg/kg SAG was shown to be effective in inhibiting tumour growth. Our research offered a theoretical foundation for the clinical antitumor properties of SAG, further suggesting its potential application in the clinic. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol

Author

Guo, Yanjing, Zhao, Meng, Bo, Tao, Ma, Shizhan, Yuan, Zhongshang, Chen, Wenbin, He, Zhao, Hou, Xu, Liu, Jun, Zhang, Zhenhai, Zhu, Qiang, Wang, Qiangxiu, Lin, Xiaoyan, Yang, Zhongli, Cui, Min, Liu, Lu, Li, Yujie, Yu, Chunxiao, Qi, Xiaoyi, Wang, Qian, Zhang, Haiqing, Guan, Qingbo, Zhao, Lifang, Xuan, Shimeng, Yan, Huili, Lin, Yanliang, Wang, Li, Li, Qihang, Song, Yongfeng, Gao, Ling, and Zhao, Jiajun

Published
2019
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33. Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol

Author

Guo, Yanjing, primary, Zhao, Meng, additional, Bo, Tao, additional, Ma, Shizhan, additional, Yuan, Zhongshang, additional, Chen, Wenbin, additional, He, Zhao, additional, Hou, Xu, additional, Liu, Jun, additional, Zhang, Zhenhai, additional, Zhu, Qiang, additional, Wang, Qiangxiu, additional, Lin, Xiaoyan, additional, Yang, Zhongli, additional, Cui, Min, additional, Liu, Lu, additional, Li, Yujie, additional, Yu, Chunxiao, additional, Qi, Xiaoyi, additional, Wang, Qian, additional, Zhang, Haiqing, additional, Guan, Qingbo, additional, Zhao, Lifang, additional, Xuan, Shimeng, additional, Yan, Huili, additional, Lin, Yanliang, additional, Wang, Li, additional, Li, Qihang, additional, Song, Yongfeng, additional, Gao, Ling, additional, and Zhao, Jiajun, additional

Published
2018
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36. Development of an Improved Three-Dimensional In VitroIntestinal Mucosa Model for Drug Absorption Evaluation

Author

Li, Na, Wang, Dandan, Sui, Zhigang, Qi, Xiaoyi, Ji, Liyun, Wang, Xiuli, and Yang, Ling

Abstract

Human epithelial cell culture models of monolayer Caco-2 cells have been widely employed to assess the absorption of drug molecules across intestinal mucosa. However, cautions should be taken when interpreting the conclusions from those models due to their undesirable phenotype and functionality when compared with the native intestinal tissue. In the present study, an improved, more physiologically relevant three-dimensional (3D) culture model of the intestinal mucosa was developed to study drug absorption, in which a coculture of epithelial cells, including Caco-2 cells and HT29-methotrexate cells, was indirectly seeded on a Transwell filter insert with collagen gel and stromal cells (fibroblasts and immunocytes) incorporation. This setting-up provided a compatible environment to improve the phenotype and functionality of the epithelial cells. Compared with the monolayer culture of Caco-2 cells, the reconstructed 3D model displayed more physiologically relevant characteristics evidenced by its decreased TEER value and mucus-like layer formation. A decreased expression of P-gp and an increased expression of BCRP were also observed in the current 3D culture model, leading to a changed secretory permeability of their substrates. More importantly, an improved correlation (R2=0.843) was obtained between the absorptive permeability across the 3D coculture model and the human absorption fraction especially for those model compounds with moderate or high permeability. Thus, this engineered 3D coculture model presents a unique, improved opportunity to evaluate drug permeability in vitro.

Published
2013
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37. Using In Vitroand Machine Learning Approaches to Determine Species-Specific Dioxin-like Potency and Congener-Specific Relative Sensitivity among Birds for Brominated Dioxin Analogues

Author

Zhang, Rui, Wu, Qiuxuan, Qi, Xiaoyi, Wang, Xiaoxiang, Zhang, Xuesheng, Song, Chao, Peng, Ying, Crump, Doug, and Zhang, Xiaowei

Abstract

There is a paucity of experimental data regarding dioxin-like toxicity of polybrominated dibenzo-p-dioxins/dibenzofurans (PBDD/Fs) and non-orthopolybrominated biphenyls (PBBs). In this study, avian aryl hydrocarbon receptor 1 (AHR1)-luciferase reporter gene assays were used to determine their species-specific dioxin-like potencies (DLPs) and congener-specific interspecies relative sensitivities in birds. The results suggested that DLPs of the brominated congeners for chicken-like (Ile324_Ser380) species did not always follow World Health Organization toxicity equivalency factors of their chlorinated analogues. For ring-necked pheasant-like (Ile324_Ala380) and Japanese quail-like (Val324_Ala380) species, the difference in DLP for several congeners was 1 or even 2 orders of magnitude. Moreover, molecular docking and molecular dynamics simulation were performed to explore the interactions between the brominated congeners and AHR1-ligand-binding domain (LBD). The molecular mechanics energy (EMM) between each congener and each individual amino acid (AA) residue in AHR1–LBD was calculated. These EMMvalues could finely characterize the final conformation of species-specific AHR1–LBD for each brominated congener. Based on this, mechanism-driven generalized linear models were successfully built using machine learning algorithms and the spline approximation method, and these models could qualitatively predict the complex relationships between AHR1 conformations and DLPs or avian interspecies relative sensitivity to brominated dioxin-like compounds (DLCs). In addition, several AAs conserved among birds were found to potentially interact with species-specific AAs, thereby inducing species-specific interactions between AHR1 and brominated DLCs. The present study provides a novel strategy to facilitate the development of mechanism-driven computational prediction models for supporting safety assessment of DLCs, as well as a basis for the ecotoxicological risk assessment of brominated congeners in birds.

Published
2021
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38. Development of an improved three-dimensional in vitro intestinal mucosa model for drug absorption evaluation.

Author

Li N, Wang D, Sui Z, Qi X, Ji L, Wang X, and Yang L

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Animals, Biological Transport, Caco-2 Cells, Cell Proliferation, Cell Shape, Cell Survival, Coculture Techniques, Electric Impedance, Embryo, Mammalian cytology, Epithelial Cells cytology, Fibroblasts cytology, HT29 Cells, Humans, Mice, Models, Biological, Permeability, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Staining and Labeling, Zonula Occludens-1 Protein metabolism, Intestinal Absorption, Intestinal Mucosa metabolism, Pharmaceutical Preparations metabolism, Tissue Culture Techniques methods
Abstract

Human epithelial cell culture models of monolayer Caco-2 cells have been widely employed to assess the absorption of drug molecules across intestinal mucosa. However, cautions should be taken when interpreting the conclusions from those models due to their undesirable phenotype and functionality when compared with the native intestinal tissue. In the present study, an improved, more physiologically relevant three-dimensional (3D) culture model of the intestinal mucosa was developed to study drug absorption, in which a coculture of epithelial cells, including Caco-2 cells and HT29-methotrexate cells, was indirectly seeded on a Transwell filter insert with collagen gel and stromal cells (fibroblasts and immunocytes) incorporation. This setting-up provided a compatible environment to improve the phenotype and functionality of the epithelial cells. Compared with the monolayer culture of Caco-2 cells, the reconstructed 3D model displayed more physiologically relevant characteristics evidenced by its decreased TEER value and mucus-like layer formation. A decreased expression of P-gp and an increased expression of BCRP were also observed in the current 3D culture model, leading to a changed secretory permeability of their substrates. More importantly, an improved correlation (R(2)=0.843) was obtained between the absorptive permeability across the 3D coculture model and the human absorption fraction especially for those model compounds with moderate or high permeability. Thus, this engineered 3D coculture model presents a unique, improved opportunity to evaluate drug permeability in vitro.

Published
2013
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