3,148 results on '"Qian, Ma"'
Search Results
2. Terahertz spoof plasmonic neural network for diffractive information recognition and processing
- Author
-
Xinxin Gao, Ze Gu, Qian Ma, Bao Jie Chen, Kam-Man Shum, Wen Yi Cui, Jian Wei You, Tie Jun Cui, and Chi Hou Chan
- Subjects
Science - Abstract
Abstract All-optical diffractive neural networks, as analog artificial intelligence accelerators, leverage parallelism and analog computation for complex data processing. However, their low space transmission efficiency or large spatial dimensions hinder miniaturization and broader application. Here, we propose a terahertz spoof plasmonic neural network on a planar diffractive platform for direct multi-target recognition. Our approach employs a spoof surface plasmon polariton coupler array to construct a diffractive network layer, resulting in a compact, efficient, and easily integrable architecture. We designed three schemes: basis vector classification, multi-user recognition, and MNIST handwritten digit classification. Experimental results reveal that the terahertz spoof plasmonic neural network successfully classifies basis vectors, recognizes multi-user orientation information, and directly processes handwritten digits using a designed input framework comprising a metal grating array, transmitters, and receivers. This work broadens the application of terahertz plasmonic metamaterials, paving the way for terahertz on-chip integration, intelligent communication, and advanced computing systems.
- Published
- 2024
- Full Text
- View/download PDF
3. N6-methyladenosine writer METTL16-mediated alternative splicing and translation control are essential for murine spermatogenesis
- Author
-
Qian Ma, Yiqian Gui, Xixiang Ma, Bingqian Zhang, Wenjing Xiong, Shiyu Yang, Congcong Cao, Shaomei Mo, Ge Shu, Jing Ye, Kuan Liu, Xiaoli Wang, Yaoting Gui, Fengli Wang, and Shuiqiao Yuan
- Subjects
Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
Abstract Background The mitosis-to-meiosis switch during spermatogenesis requires dynamic changes in gene expression. However, the regulation of meiotic transcriptional and post-transcriptional machinery during this transition remains elusive. Results We report that methyltransferase-like protein 16 (METTL16), an N6-methyladenosine (m6A) writer, is required for mitosis-to-meiosis transition during spermatogenesis. Germline conditional knockout of Mettl16 in male mice impairs spermatogonial differentiation and meiosis initiation. Mechanistically, METTL16 interacts with splicing factors to regulate the alternative splicing of meiosis-related genes such as Stag3. Ribosome profiling reveals that the translation efficiency of many meiotic genes is dysregulated in METTL16-deficient testes. m6A-sequencing shows that ablation of METTL16 causes upregulation of the m6A-enriched transcripts and downregulation of the m6A-depleted transcripts, similar to Meioc and/or Ythdc2 mutants. Further in vivo and in vitro experiments demonstrate that the methyltransferase activity site (PP185-186AA) of METTL16 is necessary for spermatogenesis. Conclusions Our findings support a molecular model wherein the m6A writer METTL16-mediated alternative splicing and translation efficiency regulation are required to control the mitosis-to-meiosis germ cell fate decision in mice, with implications for understanding meiosis-related male fertility disorders.
- Published
- 2024
- Full Text
- View/download PDF
4. Comparative efficacy of immune checkpoint inhibitors versus chemotherapy alone in diffuse pleural mesothelioma
- Author
-
Xuemei Zhang, Lele Chang, Qian Ma, Qian Zhang, Wansu Xu, and Qingwei Li
- Subjects
chemotherapy ,immunotherapy ,pleural mesothelioma ,safety ,survival ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background This study aimed to investigate the effects of immune checkpoint inhibitors (ICIs) versus chemotherapy on the prognosis of real‐world diffuse pleural mesothelioma patients in China. Methods Clinical data of 90 patients with diffuse pleural mesothelioma from 2019 to 2022 were collected from Harbin Medical University Cancer Hospital. Patients were divided into two groups: the ICIs‐treated group (n = 46) and the chemotherapy‐only group (n = 44). The efficacy and safety of immunotherapy relative to chemotherapy at different treatment stages were explored. Results The median progression‐free survival (PFS) was 10.0 and 7.0 months, and the median overall survival (OS) was 24.7 and 15.8 months in the ICIs‐treated group and the chemotherapy group, respectively. The ICIs‐treated group showed an 11% increase in objective response rate (ORR) (52.2% vs. 41.0%) and an 8.0% increase in disease control rate (DCR) (78.3% vs. 70.0%) compared to the chemotherapy group. The Kaplan–Meier curves demonstrated significant PFS (HR: 0.61; 95% CI: 0.38–0.98; p = 0.038) and OS (HR: 0.47; 95% CI: 0.26–0.86; p = 0.011) benefits of receiving immunotherapy over chemotherapy alone. Subgroup analysis according to treatment timing showed the same trend. Conclusion In patients with nonsurgical diffuse pleural mesothelioma, immunotherapy achieved better survival benefits compared to chemotherapy in both first‐ and second‐/third‐line treatments. The early addition of immunotherapy improved survival in patients with nonsurgical diffuse pleural mesothelioma.
- Published
- 2024
- Full Text
- View/download PDF
5. Distributed Nash Equilibrium Seeking for Games with Nonlinear Players via Fuzzy Adaptive Control.
- Author
-
Ying Chen, Qian Ma 0001, and Dongrui Wu
- Published
- 2024
- Full Text
- View/download PDF
6. Smart Data-Driven Proactive Push to Edge Network for User-Generated Videos.
- Author
-
Xiaoteng Ma, Qing Li 0006, Junkun Peng, Gareth Tyson, Ziwen Ye, Shisong Tang, Qian Ma, Shengbin Meng, and Gabriel-Miro Muntean
- Published
- 2024
- Full Text
- View/download PDF
7. Profit Maximization in Edge-Enabled Multimedia Data Market: A Game-Based Pricing Approach.
- Author
-
Bo Shen, Qian Ma, Gang Yang, and Ru Wang
- Published
- 2024
- Full Text
- View/download PDF
8. KEPC-Push: A Knowledge-Enhanced Proactive Content Push Strategy for Edge-Assisted Video Feed Streaming.
- Author
-
Ziwen Ye, Qing Li 0006, Chunyu Qiao, Xiaoteng Ma, Yong Jiang 0001, Qian Ma, Shengbin Meng, Zhenhui Yuan, and Zili Meng
- Published
- 2024
9. Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats
- Author
-
Andri K. Riau, Zhuojian Look, Gary H. F. Yam, Craig Boote, Qian Ma, Evelina J. Y. Han, Nur Zahirah binte M. Yusoff, Hon Shing Ong, Tze-Wei Goh, Nuur Shahinda Humaira binte Halim, and Jodhbir S. Mehta
- Subjects
Science - Abstract
Abstract Intrastromal cell therapy utilizing quiescent corneal stromal keratocytes (qCSKs) from human donor corneas emerges as a promising treatment for corneal opacities, aiming to overcome limitations of traditional surgeries by reducing procedural complexity and donor dependency. This investigation demonstrates the therapeutic efficacy of qCSKs in a male rat model of corneal stromal opacity, underscoring the significance of cell-delivery quality and keratocyte differentiation in mediating corneal opacity resolution and visual function recovery. Quiescent CSKs-treated rats display improvements in escape latency and efficiency compared to wounded, non-treated rats in a Morris water maze, demonstrating improved visual acuity, while stromal fibroblasts-treated rats do not. Advanced imaging, including multiphoton microscopy, small-angle X-ray scattering, and transmission electron microscopy, revealed that qCSK therapy replicates the native cornea’s collagen fibril morphometry, matrix order, and ultrastructural architecture. These findings, supported by the expression of keratan sulfate proteoglycans, validate qCSKs as a potential therapeutic solution for corneal opacities.
- Published
- 2024
- Full Text
- View/download PDF
10. A spatially localized DNA linear classifier for cancer diagnosis
- Author
-
Linlin Yang, Qian Tang, Mingzhi Zhang, Yuan Tian, Xiaoxing Chen, Rui Xu, Qian Ma, Pei Guo, Chao Zhang, and Da Han
- Subjects
Science - Abstract
Abstract Molecular computing is an emerging paradigm that plays an essential role in data storage, bio-computation, and clinical diagnosis with the future trends of more efficient computing scheme, higher modularity with scaled-up circuity and stronger tolerance of corrupted inputs in a complex environment. Towards these goals, we construct a spatially localized, DNA integrated circuits-based classifier (DNA IC-CLA) that can perform neuromorphic architecture-based computation at a molecular level for medical diagnosis. The DNA-based classifier employs a two-dimensional DNA origami as the framework and localized processing modules as the in-frame computing core to execute arithmetic operations (e.g. multiplication, addition, subtraction) for efficient linear classification of complex patterns of miRNA inputs. We demonstrate that the DNA IC-CLA enables accurate cancer diagnosis in a faster (about 3 h) and more effective manner in synthetic and clinical samples compared to those of the traditional freely diffusible DNA circuits. We believe that this all-in-one DNA-based classifier can exhibit more applications in biocomputing in cells and medical diagnostics.
- Published
- 2024
- Full Text
- View/download PDF
11. Dielectric-magnetic synergistic construction of 2D FeCo/Co8FeS8/C composites for efficient electromagnetic wave capture
- Author
-
Qian Ma, Rong Qiang, Yulong Shao, Xiao Yang, Yi Chen, Rui Xue, Fangjie Ren, Yuancheng Ding, Lei Rong, Jingbo Fang, Qing Miao, and Zheng Guo
- Subjects
Microwave absorption ,Salt-template ,Carbon ,FeCo ,CST simulation ,Mining engineering. Metallurgy ,TN1-997 - Abstract
Nowadays, the issue of electromagnetic pollution has become increasingly prominent, underscoring the critical importance of developing high-performance microwave absorbers. This study put forwards the synthesis process of two-dimensional (2D) FeCo/Co8FeS8/C carbon nanosheets, wherein CoSO4 and FeCl3 serve as inorganic salt templates, and dopamine functions as the carbon source. FeCo alloy nanoparticles and Co8FeS8 nanoparticles are loaded on the surface of the carbon layer. 2D structure forms the conductive network, which proves advantages in establishing efficient electronic channels and minimizing conduction losses. The carbon component increases the dielectric constant of the composite and improves its impedance matching, and the FeCo alloy provides a certain ML. The synergistic effect between these components significantly contributes to the exceptional microwave absorption performance of the composites. Accordingly, the minimum reflection loss (RLmin) of FeCo/Co8FeS8/C composite reaches −47.4 dB, its effective absorption bandwidth (EAB) reaches 5.3 GHz. In particular, CST confirms that radar cross-section reduction value of FeCo/Co8FeS8/C composite can reach 20.2 dB m2 in the range of −90°
- Published
- 2024
- Full Text
- View/download PDF
12. Boosting organic phosphorescence in adaptive host-guest materials by hyperconjugation
- Author
-
Huili Ma, Lishun Fu, Xiaokang Yao, Xueyan Jiang, Kaiqi Lv, Qian Ma, Huifang Shi, Zhongfu An, and Wei Huang
- Subjects
Science - Abstract
Abstract Phosphorescence is ubiquitous in heavy atom-containing organic phosphors, which attracts considerable attention in optoelectronics and bioelectronics. However, heavy atom-free organic materials with efficient phosphorescence are rare under ambient conditions. Herein, we report a series of adaptive host-guest materials derived from dibenzo-heterocyclic analogues, showing host-dependent color-tunable phosphorescence with phosphorescence efficiency of up to 98.9%. The adaptive structural deformation of the guests arises from the hyperconjugation, namely the n→π* interaction, enabling them to inhabit the cavity of host crystals in synergy with steric effects. Consequently, a perfect conformation match between host and guest molecules facilitates the suppression of triplet exciton dissipation, thereby boosting the phosphorescence of these adaptive materials. Moreover, we extend this strategy to a ternary host-guest system, yielding both excitation- and time-dependent phosphorescence with a phosphorescence efficiency of 92.0%. This principle provides a concise way for obtaining efficient and color-tunable phosphorescence, making a major step toward potential applications in optoelectronics.
- Published
- 2024
- Full Text
- View/download PDF
13. Transcriptome-metabolome reveals the molecular changes in meat production and quality in the hybrid populations of Sichuan white goose
- Author
-
Zhengyang Shen, Yinjuan Lu, Yuan Bai, Junpeng Li, Huazhen Wang, Daqin Kou, Zhongbin Li, Qian Ma, Jiwei Hu, Lili Bai, Liang Li, Jiwen Wang, and Hehe Liu
- Subjects
hybrid breeding ,meat quality ,Sichuan white goose ,transcriptomics ,metabolomics ,Animal culture ,SF1-1100 - Abstract
ABSTRACT: Hybrid breeding has proven to enhance meat quality and is extensively utilized in goose breeding. Nevertheless, there is a paucity of research investigating the molecular mechanisms that underlie the meat quality of hybrid geese. In this study, we employed the Sichuan White Goose as the maternal line for hybridization with the Zhedong White Goose and Tianfu Meat Goose P3 line. We assessed the growth and slaughter meat quality performance of 10-wk-old hybrid offspring in comparison to Sichuan white goose purebred offspring. The results indicate that hybrid geese have significantly improved performance in growth and slaughter meat quality. Furthermore, we conducted a comprehensive analysis of the chest muscles of hybrid offspring through transcriptomics and metabolomics to unravel the effects of hybrid breeding on growth and meat quality. A total of 673 differentially expressed genes (DEGs), and 93 differentially expressed metabolites were identified. The joint analysis highlighted the significant enrichment of DEGs AMPD1, AMPD3, RRM2, ENTPD3, and the metabolite UMP in the nucleotide metabolism pathway. These findings underscore the crucial role of these genetic and metabolic factors in regulating muscle growth and meat quality in hybrid populations.
- Published
- 2024
- Full Text
- View/download PDF
14. Dextran sulfate inhibits proliferation and metastasis of human gastric cancer cells via miR-34c-5p
- Author
-
Yuan Zhao, Qian Ma, Wenwei Gao, Zhaojun Li, Guangfu Yu, Bing Li, Yuanyi Xu, and Yunning Huang
- Subjects
Dextran sulfate ,Gastric cancer ,miR-34c-5p ,Proliferation ,Metastasis ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Gastric cancer (GC) is a malignant tumor with a high global mortality rate that is currently difficult to treat. Dextran sulfate (DS), a safe anti-tumor agent, can effectively inhibit the malignant biological behavior of gastric cancer; however, its mechanism of action is not fully understood. Therefore, this study aimed at elucidate the potential mechanisms of action. Methods: In this study we used DS to intervene in lentivirus-transfected gastric cancer cells to observe the effect of DS on miR-34c-5p. RT-qPCR, CCK-8, clone formation assay, wound healing assay, transwell assay and western blot were used to examine whether DS affects the proliferation and metastasis of gastric cancer cells via miR-34c-5p. The results were validated using in vivo experiments. Results: Our data confirmed that DS up-regulated miR-34c-5p expression in human gastric cancer cells. Moreover, DS intervention enhanced the inhibitory effect of miR-34c-5p over-expression on the proliferation, invasion, and migration of gastric cancer cells, and partially reversed the promotive effect of miR-34c-5p on the proliferation, invasion, and migration of gastric cancer cells. In addition, DS could affect the activation of the MAP2K1/ERK signaling pathway through the up-regulation of miR-34c-5p, thereby inhibiting the malignant biological behavior of gastric cancer. Finally, it was demonstrated that DS could also inhibit the expression of MAP2K1 in vivo, which in turn inhibits the activation of the ERK signaling pathway to exert anti-cancer effects. Conclusion: DS may inhibit the proliferation and metastasis of gastric cancer cells by regulating miR-34c-5p, which may be a new option for clinical treatment.
- Published
- 2024
- Full Text
- View/download PDF
15. A Comparative Study of DC Beads, Callispheres and Multimodal Imaging Nano-Assembled Microspheres Loaded with Irinotecan in Vitro
- Author
-
Jieyu Wang MD, Shaoya Zhang MD, Yiwei He MD, Wan Sun MD, Xiaoyang Zhu MD, Zihan Xi MD, Qian Ma MD, Yuanxin Ye MD, Ziyang Song MD, Yuqing Zhang PhD, and Guoliang Shao PhD
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Introduction: In recent years, the development of drug-eluting embolization beads that can be imaged has become a hot research topic in regard to meeting clinical needs. In our previous study, we successfully developed nano-assembled microspheres (NAMs) for multimodal imaging purposes. NAMs can not only be visualized under CT/MR/Raman imaging but can also load clinically required doses of doxorubicin. It is important to systematically compare the pharmacokinetics of NAMs with those of commercially available DC Beads and CalliSpheres to evaluate the clinical application potential of NAMs. Methods: In our study, we compared NAMs with two types of drug-eluting beads (DEBs) in terms of irinotecan, drug-loading capacity, release profiles, microsphere diameter variation, and morphological characteristics. Results: Our results indicate that NAMs had an irinotecan loading capacity similar to those of DC Beads and CalliSpheres but exhibited better sustained release in vitro. Conclusion: NAMs have great potential for application in transcatheter arterial chemoembolization for the treatment of colorectal cancer liver metastases.
- Published
- 2024
- Full Text
- View/download PDF
16. Current perspectives and trends of CD39-CD73-eAdo/A2aR research in tumor microenvironment: a bibliometric analysis
- Author
-
Tian Huang, Xiangqing Ren, Xiaolong Tang, Yuping Wang, Rui Ji, Qinghong Guo, Qian Ma, Ya Zheng, Zenan Hu, and Yongning Zhou
- Subjects
CD39 ,CD73 ,adenosine ,A2aR ,tumor microenvironment ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background and objectiveExtracellular adenosine (eAdo) bridges tumor metabolism and immune regulation. CD39-CD73-eAdo/A2aR axis regulates tumor microenvironment (TME) and immunotherapy response. In the era of immunotherapy, exploring the impact of the CD39-CD73-eAdo/A2aR axis on TME and developing targeted therapeutic drugs to enhance the efficacy of immunotherapy are the current research hotspots. This study summarizes and explores the research trends and hotspots of the adenosine axis in the field of TME to provide ideas for further in-depth research.MethodsLiterature information was obtained from the Web of Science core collection database. The VOS viewer and the bibliometric tool based on R were used to quantify and identify cooperation information and individual influence by analyzing the detailed information of the global annual publication volume, country/region and institution distribution, article authors and co-cited authors, and journal distribution of these articles. At the same time, the distribution of author keywords and the co-occurrence of author keywords, highly cited articles, and highly co-cited references of CD39-CD73-eAdo/A2aR in the field of TME were analyzed to determine research hotspots and trends.Result1,721 articles published in the past ten years were included in this study. Through bibliometric analysis, we found that (1) 69 countries and regions explored the effect of the CD39-CD73-eAdo/A2aR on TME, and the research was generally on the rise. Researchers in the United States dominated research in this area, with the highest total citation rate. China had the most significant number of publications. (2) Harvard University has published the most articles in this field. (3) 12,065 authors contributed to the publication of papers in this field, of which 23 published at least eight papers. STAGG J had significant academic influence, with 24 published articles and 2,776 citations. Co-cited authors can be clustered into three categories. Stagg J, Allard B, Ohta A, and Antonioli, L occupied a central position in the network. (4) 579 scholarly journals have published articles in this field. The journal FRONTIERS IN IMMUNOLOGY published the most significant number of papers, with 97 articles and a total of 2,317 citations, and the number of publications increased year by year. (5) “The ectonucleotidases CD39 and CD73: Novel checkpoint inhibitor targets” was the most frequently local cited article (163 times). The “A2A adenosine receptor protects tumors from antitumor T cells” was the most co-cited reference (224 times). (6) Through the analysis of author keywords, we found that the relationship between adenosine and immunotherapy was a core concept for many researchers in this field. Breast cancer, melanoma, colorectal cancer, ovarian cancer, glioblastoma, pancreatic cancer, hepatocellular carcinoma, and lung cancer were the most frequent cancer types in adenosine-related tumor studies. Immunotherapy, immunosuppression, immune checkpoint, and immune checkpoint inhibitors were the hot keywords in the research, reflecting the importance of the adenosine metabolic pathway in tumor immunotherapy. The keywords such as Immunogenic cell death, T cells, Sting, regulatory T cells, innate immunity, and immune infiltration demonstrated the pathways by which adenosine affected the TME. The famous author keywords in recent years have been immunotherapy, immunogenic cell death, inflammation, lung cancer, and gastric cancer.ConclusionThe effect of CD39-CD73-eAdo/A2aR on the infiltration and function of various immune cells in TME, tumor immunotherapy response, and patient prognosis has attracted the attention of researchers from many countries/regions. American scholars still dominate the research in this field, but Chinese scholars produce the most research results. The journal FRONTIERS IN IMMUNOLOGY has published the wealthiest research in the field. Stagg J was a highly influential researcher in this field. Further exploration of targeted inhibition of CD39-CD73-eAdo/A2aR alone or in combination with other immunotherapy, radiotherapy, and chemotherapy in treating various cancer types and developing effective clinical therapeutic drugs are continuous research hotspots in this field.
- Published
- 2024
- Full Text
- View/download PDF
17. HomoR-CS: A homogeneous region-based compressed sensing method for SAR tomography
- Author
-
Qian Ma, Runzhi Jiao, Yaquan Han, Haifeng Huang, Tao Lai, Peng Shen, and Qingsong Wang
- Subjects
3-D reconstruction ,TomoSAR ,Compressed sensing ,Homogeneous region ,Urban area ,Physical geography ,GB3-5030 ,Environmental sciences ,GE1-350 - Abstract
The performance of most existing tomographic synthetic aperture radar (SAR) (TomoSAR) methods that reconstruct the scene pixel-by-pixel is degraded by speckle noise and low signal-to-noise ratio. To solve these problems, we propose a homogeneous region-based compressed sensing (HomoR-CS) method for SAR tomography. This method enhances the processing approach by transitioning from the traditional pixel-by-pixel processing to the joint processing based on multiple adjacent pixels in homogeneous regions. First, a similarity measure is designed to cluster pixels that have similar electromagnetic scattering intensities and three-dimensional (3-D) positions into homogeneous regions. Then, the homogeneous region-oriented TomoSAR signal model is proposed and the solution is given. Sparse approximation at the homogeneous region level is used to estimate the elevation for every pixel. The multiple adjacent pixels within the homogeneous region are jointly processed to determine the initial elevation centers, which are used as the prior information for the subsequent accurate elevation estimation of each pixel. Finally, the experimental analyses using both simulated and measured data validate the effectiveness of the HomoR-CS method. The results show that the HomoR-CS method reduces the outliers of 3-D reconstruction results and enhances the recovery of weak scattering targets.
- Published
- 2024
- Full Text
- View/download PDF
18. Adaptive Fuzzy State-Constrained Control Without Feasibility Conditions for Nonstrict Feedback Stochastic Nonlinear Systems With Input Delay.
- Author
-
Yanru Peng, Shengyuan Xu, Baoyong Zhang, Qian Ma 0001, and Deming Yuan
- Published
- 2024
- Full Text
- View/download PDF
19. Adaptive Event-Triggered Fixed-Time Practical Tracking Control for Uncertain Nonlinear Systems.
- Author
-
Qian Ma 0001 and Yingkang Xie
- Published
- 2024
- Full Text
- View/download PDF
20. Robust Optimal Output Regulation for Nonlinear Systems With Unknown Parameters.
- Author
-
Peng Jin, Qian Ma 0001, Frank L. Lewis, and Shengyuan Xu
- Published
- 2024
- Full Text
- View/download PDF
21. Multi-Layer NMPC for Battery Thermal Management Optimization Strategy of Connected Electric Vehicle Integrated With Waste Heat Recovery.
- Author
-
Yan Ma, Qian Ma, Hao Ding, Yunfeng Hu, and Hong Chen 0003
- Published
- 2024
- Full Text
- View/download PDF
22. Further Results on Stability Analysis for Sampled-Data Systems via Refined Semi-Looped-Functional.
- Author
-
Zhaoliang Sheng, Shengyuan Xu, Qian Ma 0001, and Baoyong Zhang
- Published
- 2024
- Full Text
- View/download PDF
23. Fuzzy Fixed-Time Prescribed-Performance Tracking Control of Nonlinear Systems With Dynamic Event-Triggered Signal.
- Author
-
Wenhui Liu, Qian Ma 0001, and Shengyuan Xu
- Published
- 2024
- Full Text
- View/download PDF
24. Fuzzy-Based Fixed-Time Attitude Control of Quadrotor Unmanned Aerial Vehicle With Full-State Constraints: Theory and Experiments.
- Author
-
Haoan Jiang, Qian Ma 0001, and Jian Guo 0007
- Published
- 2024
- Full Text
- View/download PDF
25. Nonfragile Exponential Synchronization for Delayed Fuzzy Memristive Inertial Neural Networks via Memory Sampled-Data Control.
- Author
-
Runan Guo, Shengyuan Xu, Baoyong Zhang, and Qian Ma 0001
- Published
- 2024
- Full Text
- View/download PDF
26. Event-Triggered Fixed-Time Adaptive Fuzzy Control for Nontriangular Nonlinear Systems With Unknown Control Directions.
- Author
-
Guozeng Cui, Hui Xu, Jinpeng Yu 0001, Qian Ma 0001, and Muwei Jian
- Published
- 2024
- Full Text
- View/download PDF
27. Guaranteed Cost Attitude Tracking Control for Uncertain Quadrotor Unmanned Aerial Vehicle Under Safety Constraints.
- Author
-
Qian Ma 0001, Peng Jin, and Frank L. Lewis
- Published
- 2024
- Full Text
- View/download PDF
28. Distributed Nash Equilibrium Seeking for Games in Systems With Non-Symmetric Dead-Zone Inputs.
- Author
-
Ying Chen and Qian Ma 0001
- Published
- 2024
- Full Text
- View/download PDF
29. Sampled-Data Output Feedback Control for Nonlinear Systems With High-Order Nonlinearities.
- Author
-
Zhaoming Sheng, Qian Ma 0001, and Shengyuan Xu
- Published
- 2024
- Full Text
- View/download PDF
30. Collaboration in Federated Learning With Differential Privacy: A Stackelberg Game Analysis.
- Author
-
Guangjing Huang, Qiong Wu 0009, Peng Sun 0003, Qian Ma 0002, and Xu Chen 0004
- Published
- 2024
- Full Text
- View/download PDF
31. Output-Feedback Regulation of Nonlinear Networked Systems With Input Delay Under Event-Triggered and Quantized Mechanism.
- Author
-
Wenhui Liu, Qian Ma 0001, and Shengyuan Xu
- Published
- 2024
- Full Text
- View/download PDF
32. Event-Triggered Distributed Adaptive Fixed-Time Formation Control of QUAVs With Input Constraints.
- Author
-
Hui Xu, Guozeng Cui, Qian Ma 0001, and Ze Li
- Published
- 2024
- Full Text
- View/download PDF
33. Promoting Collaboration in Cross-Silo Federated Learning: Challenges and Opportunities.
- Author
-
Chao Huang 0028, Ming Tang 0006, Qian Ma 0002, Jianwei Huang 0001, and Xin Liu 0002
- Published
- 2024
- Full Text
- View/download PDF
34. Intermittent Sampled-Data Control for Local Stabilization of Neural Networks Subject to Actuator Saturation: A Work-Interval-Dependent Functional Approach.
- Author
-
Yanyan Ni, Zhen Wang 0008, Xia Huang, Qian Ma 0001, and Hao Shen 0001
- Published
- 2024
- Full Text
- View/download PDF
35. Price Competition in Multi-Server Edge Computing Networks Under SAA and SIQ Models.
- Author
-
Ziya Chen, Qian Ma 0002, Lin Gao 0001, and Xu Chen 0004
- Published
- 2024
- Full Text
- View/download PDF
36. PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1
- Author
-
Fuqiang Huang, Jing Guo, Na Zhao, Mengjie Hou, Xiaochen Gai, Shuhui Yang, Pei Cai, Yanan Wang, Qian Ma, Qi Zhao, Li Li, Huayu Yang, Yanling Jing, Di Jin, Zhongdong Hu, Xiaojun Zha, Hongyang Wang, Yilei Mao, Fangming Liu, and Hongbing Zhang
- Subjects
Hepatocellular carcinoma ,Intrahepatic cholangiocarcinoma ,Hepatitis B virus ,Pten ,GP73 ,Medicine - Abstract
Abstract Background Although hepatitis B virus (HBV) infection is a major risk factor for hepatic cancer, the majority of HBV carriers do not develop this lethal disease. Additional molecular alterations are thus implicated in the process of liver tumorigenesis. Since phosphatase and tensin homolog (PTEN) is decreased in approximately half of liver cancers, we investigated the significance of PTEN deficiency in HBV-related hepatocarcinogenesis. Methods HBV-positive human liver cancer tissues were checked for PTEN expression. Transgenic HBV, Alb-Cre and Pten fl/fl mice were inter-crossed to generate WT, HBV, Pten −/− and HBV; Pten −/− mice. Immunoblotting, histological analysis and qRT-PCR were used to study these livers. Gp73 −/− mice were then mated with HBV; Pten −/− mice to illustrate the role of hepatic tumor biomarker golgi membrane protein 73 (GP73)/ golgi membrane protein 1 (GOLM1) in hepatic oncogenesis. Results Pten deletion and HBV transgene synergistically aggravated liver injury, inflammation, fibrosis and development of mixed hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). GP73 was augmented in HBV; Pten −/− livers. Knockout of GP73 blunted the synergistic effect of deficient Pten and transgenic HBV on liver injury, inflammation, fibrosis and cancer development. Conclusions This mixed HCC-ICC mouse model mimics liver cancer patients harboring HBV infection and PTEN/AKT signaling pathway alteration. Targeting GP73 is a promising therapeutic strategy for cancer patients with HBV infection and PTEN alteration.
- Published
- 2024
- Full Text
- View/download PDF
37. A rare non-gadolinium enhancing sarcoma brain metastasis with microenvironment dominated by tumor-associated macrophages
- Author
-
David Rogawski, Joshua Wheeler, Esther Nie, William Zhu, Eleanor Villanueva, Gwen Coffey, Qian Ma, Kristen Ganjoo, Nancy Fischbein, Michael Iv, Hannes Vogel, and Seema Nagpal
- Subjects
Sarcoma ,Brain metastasis ,Tumor-associated macrophages ,Brain tumor microenvironment ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Brain metastases occur in 1% of sarcoma cases and are associated with a median overall survival of 6 months. We report a rare case of a brain metastasis with unique radiologic and histopathologic features in a patient with low grade fibromyxoid sarcoma (LGFMS) previously treated with immune checkpoint inhibitor (ICI) therapy. The lone metastasis progressed in the midbrain tegmentum over 15 months as a non-enhancing, T2-hyperintense lesion with peripheral diffusion restriction, mimicking a demyelinating lesion. Histopathology of the lesion at autopsy revealed a rich infiltrate of tumor-associated macrophages (TAMs) with highest density at the leading edge of the metastasis, whereas there was a paucity of lymphocytes, suggestive of an immunologically cold environment. Given the important immunosuppressive and tumor-promoting functions of TAMs in gliomas and carcinoma/melanoma brain metastases, this unusual case provides an interesting example of a dense TAM infiltrate in a much rarer sarcoma brain metastasis.
- Published
- 2024
- Full Text
- View/download PDF
38. Analysis of the influence of the daily regulation of power stations on navigable flow conditions at river confluences using the LSTM model
- Author
-
Hongcheng Xue, Shihao Cui, Qian Ma, Zhongyong Li, Pengyu Zhou, Yuanyuan Li, and Lingyun Xie
- Subjects
Unsteady flow ,river confluence ,numerical simulation ,deep learning ,navigational flow conditions ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The daily operations of large hydropower stations on rivers induce frequent variations in downstream water levels and flow velocities, resulting in unsteady and complex hydraulic characteristics at the confluence of the main stream and its tributaries, which adversely affect navigation safety. The continuity and momentum equations, along with the RNG k-ϵ turbulence model and the VOF model, were employed to simulate the river flow process at the confluence under unsteady flow conditions. The simulated results for water levels and velocity vector fields are in good agreement with experimental data. Variations in hydraulic characteristics, including water level, flow velocity, and longitudinal gradient at the confluence of the main stream and its tributaries, were analysed. The results indicated that the water level at the confluence decreases when the tributary merges with the main stream. As the confluence ratio increases, fluctuations in the water level at the confluence decrease. However, an increase in the staggered period results in greater fluctuations in the water level within this region. Moreover, a crescent-shaped high-speed flow region is formed at the confluence of the tributary and the main stream. As the unsteady flow discharge of the main stream increases, the relative area of this region correspondingly enlarges, reaching its maximum at peak discharge, and subsequently gradually diminishes as the discharge decreases. Based on these simulation data, a Long Short-Term Memory (LSTM) model was developed to effectively predict water levels and flow velocities, providing a more convenient and accurate method for obtaining real-time information on confluence areas than traditional mathematical and statistical approaches. This study provides novel insights into predicting flow characteristics in confluence areas, thereby offering a basis for formulating navigation safety plans.
- Published
- 2024
- Full Text
- View/download PDF
39. Deep recycling of volatile elements in the mantle: Evidence from the heterogeneous B isotope in intra-plate basalts
- Author
-
Yan-Qing Li, Hiroshi Kitagawa, Chang-Qian Ma, Eizo Nakamura, Chie Sakaguchi, Katsura Kobayashi, and Xiang-Yun Hu
- Subjects
Intra-plate basalts ,Boron isotopes ,Serpentinite ,Volatile recycling ,Mantle heterogeneity ,Geology ,QE1-996.5 - Abstract
Volatiles in the mantle are crucial for Earth’s geodynamic and geochemical evolution. Understanding the deep recycling of volatiles is key for grasping mantle chemical heterogeneity, plate tectonics, and long-term planetary evolution. While subduction transfers abundant volatile elements from the Earth’s surface into the mantle, the fate of hydrous portions within subducted slabs during intensive dehydration processes remains uncertain. Boron isotopes, only efficiently fractionating near the Earth’s surface, are valuable for tracing volatile recycling signals. In this study, we document a notably large variation in δ11B values (−14.3‰ to +8.2‰) in Cenozoic basalts from the South China Block. These basalts, associated with a high-velocity zone beneath East China, are suggested to originate from the mantle transition zone. While the majority exhibit δ11B values (−10‰ to −5‰) resembling the normal mantle, their enriched Sr-Nd-Pb isotope compositions and fluid-mobile elements imply hydrous components in their source, including altered oceanic crust and sediments. The normal δ11B values are attributed to the dehydration processes. Remarkably high δ11B values in the basalts indicate the presence of subducted serpentinites in their mantle source. A small subset of samples with low δ11B values and radiogenic isotope enrichments suggests a contribution from recycled detrital sediments, though retaining minimal volatile elements after extensive dehydration. These findings provide compelling evidence that serpentinites within subducted slabs predominantly maintain their hydrous nature during dehydration processes in subduction zones. They may transport a considerable amount of water into deep mantle reservoirs, such as the mantle transition zone.
- Published
- 2024
- Full Text
- View/download PDF
40. Segmentation of retinal microaneurysms in fluorescein fundus angiography images by a novel three-step model
- Author
-
Jing Li, Qian Ma, Mudi Yao, Qin Jiang, Zhenhua Wang, and Biao Yan
- Subjects
diabetic retinopathy ,segmentation model ,microaneurysms ,fluorescein fundus angiography ,computer-aided diagnosis ,Medicine (General) ,R5-920 - Abstract
IntroductionMicroaneurysms serve as early signs of diabetic retinopathy, and their accurate detection is critical for effective treatment. Due to their low contrast and similarity to retinal vessels, distinguishing microaneurysms from background noise and retinal vessels in fluorescein fundus angiography (FFA) images poses a significant challenge.MethodsWe present a model for automatic detection of microaneurysms. FFA images were pre-processed using Top-hat transformation, Gray-stretching, and Gaussian filter techniques to eliminate noise. The candidate microaneurysms were coarsely segmented using an improved matched filter algorithm. Real microaneurysms were segmented by a morphological strategy. To evaluate the segmentation performance, our proposed model was compared against other models, including Otsu's method, Region Growing, Global Threshold, Matched Filter, Fuzzy c-means, and K-means, using both self-constructed and publicly available datasets. Performance metrics such as accuracy, sensitivity, specificity, positive predictive value, and intersection-over-union were calculated.ResultsThe proposed model outperforms other models in terms of accuracy, sensitivity, specificity, positive predictive value, and intersection-over-union. The segmentation results obtained with our model closely align with benchmark standard. Our model demonstrates significant advantages for microaneurysm segmentation in FFA images and holds promise for clinical application in the diagnosis of diabetic retinopathy.ConclusionThe proposed model offers a robust and accurate approach to microaneurysm detection, outperforming existing methods and demonstrating potential for clinical application in the effective treatment of diabetic retinopathy.
- Published
- 2024
- Full Text
- View/download PDF
41. Omadacycline for the treatment of patients with Legionella pneumophila pneumonia after experiencing liver dysfunction: case series
- Author
-
Ani Zhu, Qian Ma, and Zhiyan Liu
- Subjects
Legionella pneumophila ,pneumonia ,omadacycline ,liver function ,case series ,Microbiology ,QR1-502 - Abstract
IntroductionAntibiotics frequently induce abnormal liver function. Omadacycline is a novel aminomethylcycline antibiotic, which shows potent activity against Gram-positive and Gram-negative aerobic, anaerobic, and atypical (including Legionella pneumophila) bacteria. Of note, omadacycline is tolerable in most patients with liver impairment. However, evidence regarding the application of omadacycline in patients with Legionella pneumophila pneumonia after experiencing liver dysfunction is scarce.MethodsThe current study reported 6 cases of patients with Legionella pneumophila pneumonia receiving omadacycline as subsequent antibiotics after experiencing liver dysfunction.ResultsThese 6 cases were admitted to the hospital for pneumonia and received antibiotic therapy, including piperacillin-tazobactam, imipenem, meropenem, and moxifloxacin. After receiving these antibiotics, increased liver enzymes were noted. Although hepatoprotective therapy (such as magnesium isoglycyrrhizinate and glutathione) was given, the liver function was still abnormal. According to metagenomic next-generation sequencing, these patients were diagnosed with Legionella pneumophila pneumonia. Considering the abnormal liver function, the antibiotic therapy was switched to omadacycline-containing antibiotic therapy. After that, liver function was improved, and the infection was ameliorated. Ultimately, all patients discharged from the hospital, including 2 patients who achieved complete clinical symptomatic improvement and 4 patients who achieved partial clinical symptomatic improvement.DiscussionThis study emphasizes the successful treatment of switching to omadacycline after experiencing abnormal liver function in patients with Legionella pneumophila pneumonia. This study suggests that omadacycline may serve as an optional antibiotic for patients with Legionella pneumophila pneumonia, especially when occurring liver dysfunction. However, more clinical studies are required to validate our findings.
- Published
- 2024
- Full Text
- View/download PDF
42. A severe case of iatrogenic botulism associated with COVID-19 infection
- Author
-
Qian Ma, Guang-Jing Liu, Shuang Wang, Wen-Di Wang, Ning Liu, Che-Jiang Wang, and Xiao-Bing Li
- Subjects
Botulism ,Botulinum toxin ,COVID-19 infection ,Plastic surgery injection ,Respiratory failure ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: The botulinum toxin is an extremely potent substance that impacts the nervous system. There has been a rise in cases of medical poisoning associated with it, particularly in the field of plastic and aesthetic procedures, in recent years. Case description: A 51-year-old woman underwent a facial wrinkle reduction procedure with an unauthorized injection of 100 U of botulinum toxin at an unlicensed medical facility six days prior to hospitalization. Over time, her toxicity symptoms intensified, impacting her respiratory muscles, and she did not receive antitoxin treatment. She was concurrently diagnosed with a COVID-19 infection during this period. Nonetheless, she experienced a full recovery 86 days after the injection. Conclusion: Currently, there is no effective antidote for botulism. Nevertheless, the timely administration of antitoxin can contribute to reducing the duration of the illness, alleviating symptoms, and preventing its recurrence. It is essential to recognize that individual responses may vary, and in this instance, the absence of antitoxin treatment did not significantly prolong the course of the disease. Accurate diagnosis of medical poisoning can be based on injection history and clinical symptoms. Early indications like fatigue and dry mouth warrant particular attention, emphasizing the importance of immediate medical intervention. To address emergencies, the Center for Disease Control (CDC) should maintain an accessible supply of antitoxin. Patients with severe poisoning should be hospitalized until their respiratory muscle strength is fully restored.
- Published
- 2024
- Full Text
- View/download PDF
43. Clinical value of video oculomotor evaluation in the differential diagnosis of multiple system atrophy and Parkinson's disease
- Author
-
Dongxiao Zhou, Qian Ma, Haiwei Huang, and Xue Xu
- Subjects
multiple system atrophy ,Parkinson's disease ,video oculomotor evaluation ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Abstract Background Multiple system atrophy (MSA) is a neurodegenerative disease that progresses rapidly and has a poor prognosis. This study aimed to assess the value of video oculomotor evaluation (VOE) in the differential diagnosis of MSA and Parkinson's disease (PD). Methods In total, 28 patients with MSA, 31 patients with PD, and 30 age‐ and sex‐matched healthy controls (HC) were screened and included in this study. The evaluation consisted of a gaze‐holding test, smooth pursuit eye movement (SPEM), random saccade, and optokinetic nystagmus (OKN). Results The MSA and PD groups had more abnormalities and decreased SPEM gain than the HC group (64.29%, 35.48%, 10%, p
- Published
- 2024
- Full Text
- View/download PDF
44. Ultrasound modification on milk fat globule membrane and soy lecithin to improve the physicochemical properties, microstructure and stability of mimicking human milk fat emulsions
- Author
-
Qian Ma, Tao Zhou, Zhong Wang, Yanjie Zhao, Xiaodong Li, Lu Liu, Xiuxiu Zhang, Kouadio Jean Eric-Parfait Kouame, and Shuo Chen
- Subjects
Ultrasound ,Milk fat globules membrane ,Soybean lecithin ,Mimicking MFG emulsion ,Stability ,Structure ,Chemistry ,QD1-999 ,Acoustics. Sound ,QC221-246 - Abstract
Starting from the consideration of the structure of human milk fat globule (MFG), this study aimed to investigate the effects of ultrasonic treatment on milk fat globule membrane (MFGM) and soy lecithin (SL) complexes and their role in mimicking human MFG emulsions. Ultrasonic power significantly affected the structure of the MFGM-SL complex, further promoting the unfolding of the molecular structure of the protein, and then increased solubility and surface hydrophobicity. Furthermore, the microstructure of mimicking MFG emulsions without sonication was unevenly distributed, and the average droplet diameter was large. After ultrasonic treatment, the droplets of the emulsion were more uniformly dispersed, the particle size was smaller, and the emulsification properties and stability were improved to varying degrees. Especially when the ultrasonic power was 300 W, the mimicking MFG emulsion had the highest encapsulation rate and emulsion activity index and emulsion stability index were increased by 60.88 % and 117.74 %, respectively. From the microstructure, it was observed that the spherical droplets of the mimicking MFG emulsion after appropriate ultrasonic treatment remain well separated without obvious flocculation. This study can provide a reference for the screening of milk fat globules mimicking membrane materials and the further utilization and development of ultrasound in infant formula.
- Published
- 2024
- Full Text
- View/download PDF
45. Blockade of endothelial adenosine receptor 2 A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition
- Author
-
Yongfeng Cai, Yaqi Zhou, Qiuhua Yang, Jiean Xu, Qingen Da, Qian Ma, Dingwei Zhao, Tammy Lu, Ha Won Kim, David Fulton, Xuejun Jiang, Neal L. Weintraub, Kunzhe Dong, Suowen Xu, Mei Hong, Zhiping Liu, and Yuqing Huo
- Subjects
Adenosine receptor 2 A ,Endothelial to mesenchymal transition ,Atherosclerosis ,ALK5 ,CREB ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cardiovascular diseases (CVDs) are the leading cause of death worldwide, and morbidity and mortality rates continue to rise. Atherosclerosis constitutes the principal etiology of CVDs. Endothelial injury, inflammation, and dysfunction are the initiating factors of atherosclerosis. Recently, we reported that endothelial adenosine receptor 2 A (ADORA2A), a G protein-coupled receptor (GPCR), plays critical roles in neovascularization disease and cerebrovascular disease. However, the precise role of endothelial ADORA2A in atherosclerosis is still not fully understood. Here, we showed that ADORA2A expression was markedly increased in the aortic endothelium of humans with atherosclerosis or Apoe-/- mice fed a high-cholesterol diet. In vivo studies unraveled that endothelial-specific Adora2a deficiency alleviated endothelial-to-mesenchymal transition (EndMT) and prevented the formation and instability of atherosclerotic plaque in Apoe-/- mice. Moreover, pharmacologic inhibition of ADORA2A with KW6002 recapitulated the anti-atherogenic phenotypes observed in genetically Adora2a-deficient mice. In cultured human aortic endothelial cells (HAECs), siRNA knockdown of ADORA2A or KW6002 inhibition of ADORA2A decreased EndMT, whereas adenoviral overexpression of ADORA2A induced EndMT. Mechanistically, ADORA2A upregulated ALK5 expression via a cAMP/PKA/CREB axis, leading to TGFβ-Smad2/3 signaling activation, thereby promoting EndMT. In conclusion, these findings, for the first time, demonstrate that blockade of ADORA2A attenuated atherosclerosis via inhibition of EndMT induced by the CREB1-ALK5 axis. This study discloses a new link between endothelial ADORA2A and EndMT and indicates that inhibiting endothelial ADORA2A could be an effective novel strategy for the prevention and treatment of atherosclerotic CVDs.
- Published
- 2024
- Full Text
- View/download PDF
46. Geochemical characteristics and hydrocarbon generation modeling of the Paleogene source rocks in the Qinnan Depression, Bohai Bay Basin, China
- Author
-
Chuang Lei, Lingjian Meng, Zehua Tian, Qian Ma, Qi Wang, Jiang Fu, and Yaqiong Wei
- Subjects
geological analogy ,source rock ,basin modeling ,thermal history ,hydrocarbon generation history ,low-exploration area ,Science - Abstract
Introduction: The Qinnan Depression in the Bohai Bay Basin is lowly explored, where hydrocarbon generation potential of source rocks is required detailed investigation to identify hydrocarbon exploration potential and direction.Methods: This study is based on organic geochemical analysis performed on the Paleogene source rocks. After that, the burial, thermal maturity, and hydrocarbon generation histories of the Paleogene source rocks at various sags were reconstructed on BasinMod software based on reasonable geological models and geological parameters derived from geological analogy.Results: Results show that the 3rd member (Es3) and 1st member (Es1) of the Eocene Shahejie Formation are high-quality source rocks with a mixture of type I and II kerogen, whose organic matter was originated from aquatic organisms under a reducing environment. The 3rd member (Ed3) of the Oligocene Dongying Formation are fair-quality source rocks with type II kerogen and are dominated by mixed organic matter depositing in a weakly reducing environment. Clear differences in hydrocarbon generation were observed in these three source rocks owing to differential subsidence and sedimentation among the Eastern, Southeastern, and Western sags. Source rocks at the Eastern Sag were maturated early and lasted for a long time, which were currently at late mature (1.0–1.3%Ro) to high mature (1.3–2.0%Ro) stages. Two hydrocarbon generation events occurred in the Oligocene and Miocene, respectively, with intensive hydrocarbon generation capacity. Source rocks at the Southeastern Sag were maturated late and lasted for a short period, which were currently at a medium mature (0.7–1.0%Ro) stage. The major hydrocarbon generation event had occurred since the late Miocene, with intensive hydrocarbon generation capacity. Source rocks at the Western Sag were at an early mature (0.5–0.7%Ro) stage and began to generate hydrocarbon in the Pliocene, with weak hydrocarbon generation capacity. The hydrocarbon generation capacity of the Paleogene source rocks is as follows: Es3>Es1>Ed3.Discussion: The Qinnan Depression has high oil and gas exploration potential, where exploration activities should focus on effective traps around depression and slope zones located at the Eastern and Southeastern sags.
- Published
- 2024
- Full Text
- View/download PDF
47. Changes in seminal plasma microecological dynamics and the mechanistic impact of core metabolite hexadecanamide in asthenozoospermia patients
- Author
-
Baoquan Han, Yongyong Wang, Wei Ge, Junjie Wang, Shuai Yu, Jiamao Yan, Lei Hua, Xiaoyuan Zhang, Zihui Yan, Lu Wang, Jinxin Zhao, Cong Huang, Bo Yang, Yan Wang, Qian Ma, Yong Zhao, Hui Jiang, Yunqi Zhang, Shaolin Liang, Jianjuan Zhao, Zhongyi Sun, Wei Shen, and Yaoting Gui
- Subjects
16s rDNA sequencing ,asthenozoospermia ,hexadecanamide ,multi‐omics analysis ,seminal plasma metabolome ,seminal plasma microbiota ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Abstract Asthenozoospermia (AZS) is a prevalent contributor to male infertility, characterized by a substantial decline in sperm motility. In recent years, large‐scale studies have explored the interplay between the male reproductive system's microecology and its implications for reproductive health. Nevertheless, the direct association between seminal microecology and male infertility pathogenesis remains inconclusive. This study used 16S rDNA sequencing and multi‐omics analysis to conduct a comprehensive investigation of the seminal microbial community and metabolites in AZS patients. Patients were categorized into four distinct groups: Normal, mild AZS (AZS‐I), moderate AZS (AZS‐II), and severe AZS (AZS‐III). Microbiome differential abundance analysis revealed significant differences in microbial composition and metabolite profiles within the seminal plasma of these groups. Subsequently, patients were classified into a control group (Normal and AZS‐I) and an AZS group (AZS‐II and AZS‐III). Correlation and cross‐reference analyses identified distinct microbial genera and metabolites. Notably, the AZS group exhibited a reduced abundance of bacterial genera such as Pseudomonas, Serratia, and Methylobacterium‐Methylorubrum in seminal plasma, positively correlating with core differential metabolite (hexadecanamide). Conversely, the AZS group displayed an increased abundance of bacterial genera such as Uruburuella, Vibrio, and Pseudoalteromonas, with a negative correlation with core differential metabolite (hexadecanamide). In vitro and in vivo experiments validated that hexadecanamide significantly enhanced sperm motility. Using predictive metabolite‐targeting gene analysis and single‐cell transcriptome sequencing, we profiled the gene expression of candidate target genes PAOX and CA2. Protein immunoblotting techniques validated the upregulation protein levels of PAOX and CA2 in sperm samples after hexadecanamide treatment, enhancing sperm motility. In conclusion, this study uncovered a significant correlation between six microbial genera in seminal plasma and the content of the metabolite hexadecanamide, which is related to AZS. Hexadecanamide notably enhances sperm motility, suggesting its potential integration into clinical strategies for managing AZS, providing a foundational framework for diagnostic and therapeutic advancements.
- Published
- 2024
- Full Text
- View/download PDF
48. Accurate Analysis of Multi-Mode Interferometric Optical Fiber Sensor
- Author
-
Lijun Li, Congying Jia, Qian Ma, and Tianzong Xu
- Subjects
Window function ,FFT ,optical fiber interferometric sensor ,analysis method ,Applied optics. Photonics ,TA1501-1820 - Abstract
Abstract In view of the problem that the sensing characteristics of the multi-mode interferometric fiber sensors cannot be accurately analyzed, an analysis method based on the fast Fourier transform (FFT) and inverse fast Fourier transform (IFFT) is proposed and demonstrated theoretically and experimentally. The suitabilities of the rectangular window function with the narrow main lobe (high spectrum resolution) and low side lobe (high main mode energy leakage) and the Hanning window function with the wide main lobe (low spectrum resolution) and high side lobe (high energy concentration) in this kind of sensor analysis are discussed, respectively. This method can not only realize the sensing performance analysis of the various modes, but also overcome the inconsistency of the different interference wavelength (dip) sensing characteristics in the conventional analysis methods. At the same time, this method is also beneficial to solve the repetitive problem of such sensors.
- Published
- 2023
- Full Text
- View/download PDF
49. S9.6-based hybrid capture immunoassay for pathogen detection
- Author
-
Ankur Bothra, Megan L. Perry, Elena Wei, Mahtab Moayeri, Qian Ma, Marco A. Biamonte, Marina Siirin, and Stephen H. Leppla
- Subjects
Medicine ,Science - Abstract
Abstract The detection of pathogens is critical for clinical diagnosis and public health surveillance. Detection is usually done with nucleic acid-based tests (NATs) and rapid antigen tests (e.g., lateral flow assays [LFAs]). Although NATs are more sensitive and specific, their use is often limited in resource-poor settings due to specialized requirements. To address this limitation, we developed a rapid DNA-RNA Hybrid Capture immunoassay (HC) that specifically detects RNA from pathogens. This assay utilizes a unique monoclonal antibody, S9.6, which binds DNA-RNA hybrids. Biotinylated single-stranded DNA probes are hybridized to target RNAs, followed by hybrid capture on streptavidin and detection with S9.6. The HC-ELISA assay can detect as few as 104 RNA molecules that are 2.2 kb in length. We also adapted this assay into a LFA format, where captured Bacillus anthracis rpoB RNA of 3.5 kb length was detectable from a bacterial load equivalent to 107 CFU per 100 mg of mouse tissue using either HC-ELISA or HC-LFA. Importantly, we also demonstrated the versatility of HC by detecting other pathogens, including SARS-CoV-2 and Toxoplasma gondii, showing its potential for broad pathogen detection. Notably, HC does not require amplification of the target nucleic acid and utilizes economical formats like ELISA and LFA, making it suitable for use in sentinel labs for pathogen detection or as a molecular tool in basic research laboratories. Our study highlights the potential of HC as a sensitive and versatile method for RNA-based pathogen detection.
- Published
- 2023
- Full Text
- View/download PDF
50. Hypoxic tumor cell-derived small extracellular vesicle miR-152-3p promotes cervical cancer radioresistance through KLF15 protein
- Author
-
Junying Zhou, Ningjing Lei, Wanjia Tian, Ruixia Guo, Feng Gao, Hanlin Fu, Jing Zhang, Shiliang Dong, Mengyu Chen, Qian Ma, Yong Li, and Lei Chang
- Subjects
Cervical cancer ,Radioresistance ,Hypoxia ,Extracellular vesicle ,miR-152-3p ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Radiotherapy is widely used in treating cervical cancer patients, however, radioresistance unavoidably occurs and seriously affects the treatment effect. It is well known that hypoxia plays an important role in promoting radioresistance in tumor microenvironment, yet our understanding of the effect of small extracellular vesicles miRNA on cervical cancer radiosensitivity in hypoxic environment is still limited. Methods Small extracellular vesicles extracted from hypoxic and normoxic cultured cervical cancer cells were evaluated for their effects on radioresistance. miR-152-3p was found to be a potential effector in hypoxia-derived extracellular vesicles by searching the GEO database. Its downstream substrate was confirmed by double luciferase report, which was KLF15. The role of miR-152-3p and KLF15 in regulating cervical cancer radioresistance was detected by cell activity assays. The findings were confirmed in vivo by animal models. The expression of miR-152-3p was quantified by qRT-PCR and its prognostic significance was evaluated. Results Hypoxic environment promoted the secretion of small extracellular vesicles, and reduced the apoptosis and DNA damage caused by radiation, accompanied by increased expression of small extracellular vesicles miR-152-3p from hypoxic cervical cancer cells. Furthermore, small extracellular vesicles miR-152-3p promoted Hela xenograft growth and reduced the radiosensitivity vivo. Mechanism studies revealed that KLF15 protein was the downstream target of miR-152-3p in regulating radioresistance. Conclusion Our findings suggest that small extracellular vesicles miR-152-3p affects the therapeutic effect of radiotherapy and holds potential as a biomarker or therapeutic target for cervical cancer prognosis and improving radiotherapy.
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.