106 results on '"Qianfeng Xia"'
Search Results
2. Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis
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Yu Sun, Chen Chen, Chenghong Zeng, Qianfeng Xia, Chuanfei Yuan, and Hua Pei
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SFTSV ,Gut microbiota ,Ticks ,Haemaphysalis longicornis ,Sphingomonas ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Ticks serve as vectors for a diverse array of pathogens, including viruses responsible for both human and livestock diseases. Symbiotic bacteria hold significant potential for controlling tick-borne disease. However, the alteration of tick gut bacterial community in response to pathogen infection has not been analyzed for any tick-borne viruses. Here, the impact of severe fever with thrombocytopenia syndrome virus (SFTSV) infection on bacterial diversity in the gut of Haemaphysalis longicornis is investigated. Methods Unfed tick females were artificially infected with SFTSV. The gut samples were collected and the genomic DNA was extracted. We then investigated alterations in gut bacterial composition in response to SFTSV infection through 16S rRNA gene sequencing. Results The study found that a reduction in the number of operational taxonomic units (OTUs) in the tick gut following SFTSV infection. However, there were no significant changes in alpha diversity indices upon infection. Four genera, including Corynebacterium, Arthrobacter, Sphingomonas, and Escherichia, were identified as biomarkers for the tick gut without SFTSV infection. Notably, the predicted correlation network indicated that the biomarkers Sphingomonas and Escherichia exhibited positive correlations within the same subcommunity, which was altered upon viral infection. Conclusions These findings revealed that the change in tick gut bacterial composition upon SFTSV infection and could facilitate the discovery new target for tick-borne viral disease control. Graphical Abstract
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- 2024
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3. High electrochemical performance of glucose detection based on tapered gold nanostructures and MXene layers
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Xianglong Bian, Dong Yang, Yingfei Zeng, Tao Yang, Qianfeng Xia, and Tingwei Hu
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Glucose detection, Electrochemical sensor ,MXene layers ,Tapered gold nanostructures (AuTNs) ,Instruments and machines ,QA71-90 - Abstract
Diabetes disease caused by hyperglycemia has many complications, including cardiovascular disease, kidney disease and visual impairment. Effective and stable platform of enzyme-free glucose detection is significant important for the monitoring of diabetes disease. In this work, uniform single MXene layers were fabricated with large scale through HCl/LiF etching and tapered gold nanostructures (AuTNs) was electrodeposited on the MXene layers. The AuTNs with three-dimensional conical apex on the MXene layers can effectively increase the specific surface ratio and active sites. The composite materials of AuTNs and MXene layers assembled on the glassy carbon electrode (GCE) can significantly increase the electrochemical performance during glucose detection. The modified electrode of AuTNs/MXene/GCE shows good linearity from 0.1 nM to 10.0 mM, low limit of detection (LOD) of 1.43 nM and fast response time of 1.0 s, exhibiting high sensitivity, good stability and high selectivity for glucose during electrochemical detection. The high performance of the modified electrode provides promising potential application in enzyme-free sensor for the electrochemical detection of glucose.
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- 2024
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4. The efficacy and safety of remdesivir alone and in combination with other drugs for the treatment of COVID-19: a systematic review and meta-analysis
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Chuizhe Chen, Junde Fang, Shu Chen, Mamy Jayne Nelly Rajaofera, Xuemiao Li, Bo Wang, and Qianfeng Xia
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Remdesivir ,COVID-19 ,SARS-CoV-2 ,Treatment ,Meta-analysis ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Remdesivir is considered to be a specific drug for treating coronavirus disease 2019. This systematic review aims to evaluate the clinical efficacy and risk of remdesivir alone and in combination with other drugs. Research design and methods The PubMed, Embase, SCIE, Cochrane Library, and American Clinical trial Center databases were searched up to 1 April 2022 to identify. Randomized controlled trials (RCTs) and observational studies comparing the efficacy of remdesivir monotherapy and combination therapy with that of control drugs. Results Ten RCTs and 32 observational studies were included in the analysis. Regarding the primary outcome, remdesivir use reduced mortality in patients with severe COVID-19 (RR = 0.57, 95% CI (0.48,0.68)) and shortened the time to clinical improvement (MD = -2.51, 95% CI (-2.75, -2.28)). Regarding other clinical outcomes, remdesivir use was associated with improved clinical status (RR = 1.08, 95%CI (1.01, 1.17)). Regarding safety outcomes, remdesivir use did not cause liver or kidney damage (RR = 0.87, 95%CI (0.68, 1.11)) (RR = 0.88, 95%CI (0.70,1.10)). Compared with remdesivir alone, remdesivir combined with other drugs (e.g., steroids, favipiravir, and convalescent plasma) had no effect on mortality. Conclusion The use of remdesivir can help to reduce the mortality of patients with severe COVID-19 and shorten the time to clinical improvement. There was no benefit of remdesivir combination therapy for other clinical outcomes. Trial registration PROSPERO registration number: CRD42022322859.
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- 2023
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5. Potential mosquito-associated melioidosis and analysis of sample processing results in Hainan, China, 2023
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Xun Kang, Shaowen Cheng, Rui Zheng, Hengjie Zhu, and Qianfeng Xia
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Arctic medicine. Tropical medicine ,RC955-962 - Published
- 2024
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6. Association of virome dynamics with mosquito species and environmental factors
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Qing Liu, Feng Cui, Xiang Liu, Yumei Fu, Wenjing Fang, Xun Kang, Hong Lu, Siping Li, Biao Liu, Wei Guo, Qianfeng Xia, Le Kang, and Feng Jiang
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Mosquito ,Virome ,RNA virus ,Food source ,Environment ,Microbial ecology ,QR100-130 - Abstract
Abstract Background The pathogenic viruses transmitted by mosquitoes cause a variety of animal and human diseases and public health concerns. Virome surveillance is important for the discovery, and control of mosquito-borne pathogenic viruses, as well as early warning systems. Virome composition in mosquitoes is affected by mosquito species, food source, and geographic region. However, the complex associations of virome composition remain largely unknown. Results Here, we profiled the high-depth RNA viromes of 15 species of field-caught adult mosquitoes, especially from Culex, Aedes, Anopheles, and Armigeres in Hainan Island from 2018 to 2020. We detected 57 known and 39 novel viruses belonging to 15 families. We established the associations of the RNA viruses with mosquito species and their foods, indicating the importance of feeding acquisition of RNA viruses in determining virome composition. A large fraction of RNA viruses were persistent in the same mosquito species across the 3 years and different locations, showing the species-specific stability of viromes in Hainan Island. In contrast, the virome compositions of single mosquito species in different geographic regions worldwide are visibly distinct. This is consistent with the differences in food sources of mosquitoes distributed broadly across continents. Conclusions Thus, species-specific viromes in a relatively small area are limited by viral interspecific competition and food sources, whereas the viromes of mosquito species in large geographic regions may be governed by ecological interactions between mosquitoes and local environmental factors. Video Abstract
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- 2023
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7. Synchronous detection of Burkholderia pseudomallei and its ceftazidime resistance mutation based on RNase-HII hydrolysis combined with lateral flow strip assay
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Juan Yao, Zhang Zhang, Shen Tian, Nini Luo, Jun Tan, Yue Zhang, Shuo Gu, and Qianfeng Xia
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melioidosis ,Burkholderia pseudomallei ,ceftazidime resistance mutation ,rhPCR ,lateral chromatographic flow strip ,universal probes ,Microbiology ,QR1-502 - Abstract
ABSTRACT Melioidosis is a severe and often fatal infectious disease caused by Burkholderia pseudomallei, which poses significant challenges due to its high mortality rate and frequent misdiagnosis. To effectively treat infected patients, especially in epidemic regions like Hainan with a high resistance rate to ceftazidime (CAZ), early diagnosis and resistance testing are critical. Here, we designed a strategy that combines RNase-HII-dependent PCR with lateral flow strip assay (LFSA) for simultaneous detection of B. pseudomallei and its CAZ resistance mutation. In this strategy, we utilized rhPCR technology to design specific primers with a C3-spacer modification. These primers target a 115-base pair region within the ORF2 of the B. pseudomallei type III secretion system gene cluster, as well as the P174L mutation in the penA gene, which is a major mutation associated with CAZ resistance in Hainan. The primers contain complementary RNA bases that can be hydrolyzed only in the presence of thermostable RNase-HII enzymes, facilitating subsequent PCR reactions. Additionally, we introduced two pairs of universal probes carrying fluorescein isothiocyanate (FITC) and biotin, as well as digoxin and biotin. The sequences of these universal probes align with specific regions of the primers, resulting in target products labeled with different tags. The resulting amplification products are then subjected to LFSA, where the presence of specific markers is visually interpreted based on color development. These markers flow to test line 1 (T1) and are captured by a fixed anti-FITC antibody, generating a red line that indicates the presence of B. pseudomallei. Similarly, the occurrence of a red line on test line 2 (T2), which is fixed with anti-digoxin antibodies, indicates the presence of the P174L mutation in B. pseudomallei. This protocol serves as an excellent tool for the rapid diagnosis of melioidosis and provides a basis for selecting appropriate antibiotic medication. It has the potential to revolutionize the diagnosis of melioidosis, particularly in resource-limited settings. Furthermore, this technique holds promise as a versatile tool for disease diagnosis in general. IMPORTANCE This study focused on the development of a reaction system using rhPCR to amplify a specific gene, ORF2, of B. pseudomallei and to identify the P174L mutation associated with increased drug resistance to ceftazidime (CAZ). The system incorporated universal primer probes and a simple temperature cycle reaction. The amplified products were then analyzed using lateral flow strip assay (LFSA) for strain identification and mutation interpretation. The developed system provides a reliable basis for diagnosing melioidosis and selecting appropriate drugs. Its potential impact is particularly significant in resource-limited settings where access to advanced diagnostic techniques is limited. This platform stands out for its simplicity, convenience, sensitivity, specificity, and portability. It shows promise as a point-of-care testing method for detecting single nucleotide polymorphism in genes associated with other diseases. By leveraging the advantages of this platform, researchers and healthcare professionals can potentially expand its use beyond melioidosis and apply it to the rapid detection of genetic variations in other disease-related genes.
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- 2023
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8. Hedgehogs as Amplifying Hosts of Severe Fever with Thrombocytopenia Syndrome Virus, China
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Chaoyue Zhao, Xing Zhang, Xiaoxi Si, Ling Ye, Kevin Lawrence, Yajun Lu, Chunhong Du, Haidong Xu, Qian Yang, Qianfeng Xia, Guoxiang Yu, Wei Xu, Fei Yuan, Junfeng Hao, Jia-Fu Jiang, and Aihua Zheng
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severe fever with thrombocytopenia syndrome ,SFTSV ,severe fever with thrombocytopenia syndrome virus ,viruses ,bandavirus ,hedgehog ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tickborne bandavirus mainly transmitted by Haemaphysalis longicornis ticks in East Asia, mostly in rural areas. As of April 2022, the amplifying host involved in the natural transmission of SFTSV remained unidentified. Our epidemiologic field survey conducted in endemic areas in China showed that hedgehogs were widely distributed, had heavy tick infestations, and had high SFTSV seroprevalence and RNA prevalence. After experimental infection of Erinaceus amurensis and Atelerix albiventris hedgehogs with SFTSV, we detected robust but transitory viremias that lasted for 9–11 days. We completed the SFTSV transmission cycle between hedgehogs and nymph and adult H. longicornis ticks under laboratory conditions with 100% efficiency. Furthermore, naive H. longicornis ticks could be infected by SFTSV-positive ticks co-feeding on naive hedgehogs; we confirmed transstadial transmission of SFTSV. Our study suggests that the hedgehogs are a notable wildlife amplifying host of SFTSV in China.
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- 2022
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9. Identification of candidate blood biomarkers for the diagnosis of septicaemic melioidosis based on WGCNA
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Li Yin, Yuanyuan Chen, Tingting Fu, Lin Liu, and Qianfeng Xia
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Sepsis ,WGCNA ,biomarker ,B. pseudomallei ,cell killing ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Melioidosis is an infectious disease caused by Burkholderia pseudomallei (Bp), a gram-negative bacillus. Sepsis is the most prevalent type of melioidosis. Due to factors such as lack of precision and slow presentation of bacterial culture tests, the misdiagnosis rate could exceed 100 per cent. Therefore, more reliable, and adaptable diagnostic methods are urgently needed. Weighted gene co-expression network analysis (WGCNA) was employed to screen the featured modules specially expressed in sepsis patients caused by Bp. Two representative co-expression modules were selected to perform gene ontology(GO) and KEGG analysis using ClusterProfiler package based on R language. We found that antigen processing and presentation of exogenous peptide antigen via MHC class I pathway, cytosol to ER transport and cell killing related pathways enriched in darkmagenta module which significantly correlated with the sepsis caused by Bp. Eventually, a diagnostic 6-mRNA signature consisting of ASPHD2, LAP3, SEPT4, FAM26F, WARS and LGALS3BP was identified, which could discern the sepsis caused by Bp compared with other organisms. This will provide a new insight in screening markers for early detection of sepsis caused by Bp, and the interaction between pathogens and hosts. This should shed light on the early detection of Bp-caused infectious diseases.
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- 2022
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10. A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics
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Yanshuang Wang, Xuemiao Li, David A. B. Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, and Qianfeng Xia
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Phage ,Podovirus ,Burkholderia pseudomallei ,Melioidosis ,Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China. Methods Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated, in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test. Results A phage able to lyse 24/25 clinical isolates was recovered. It was classified in the Podoviridae family and was found to be amenable to propagation. Under the optimal multiplicity of infection (MOI) of 0.1, an eclipse period of around 20 min and a high titer (1012 PFU/ml) produced within 1 h were demonstrated. This phage was found stabile at a wide range of temperatures (24, 37, 40, 50, and 60 °C) and pH values (3–12). After being designated as vB_BpP_HN01, it was fully sequenced, and the 71,398 bp linear genome, containing 93 open reading frames and a tRNA-Asn, displayed a low sequence similarity with known viruses. Additionally, protective effects of applications of vB_BpP_HN01 (MOI = 0.1 and MOI = 1) alone or in combination with antibiotics were found to improve viability of infected cells (70.6 ± 6.8%, 85.8 ± 5.7%, 91.9 ± 1.8%, and 96.8 ± 1.8%, respectively). A significantly reduced mortality (10%) and a decreased pathogen load were demonstrated in infected C. elegans following the addition of this phage. Conclusions As the first B. pseudomallei phage was isolated in Hainan, China, phage vB_BpP_HN01 was characterized by promising lytic property, stability, and efficiency of bacterial elimination during the in vitro/vivo experiments. Therefore, we can conclude that it is a potential alternative agent for combating melioidosis. Graphical Abstract
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- 2022
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11. Glycometabolism change during Burkholderia pseudomallei infection in RAW264.7 cells by proteomic analysis
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Xuexia Li, Yingfei Zeng, Shengnan Guo, Chen Chen, Lin Liu, and Qianfeng Xia
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Medicine ,Science - Abstract
Abstract Burkholderia pseudomallei is a Gram-negative intracellular bacterium that causes melioidosis, a life-threatening disease. The interaction of B. pseudomallei with its host is complicated, and cellular response to B. pseudomallei infection is still largely unknown. In this study, we aimed to determine host-cell responses to B. pseudomallei at the proteomics level. We performed proteomic profiling of B. pseudomallei HNBP001-infected mouse macrophage RAW264.7 cells to characterize the cellular response dynamics during infection. Western blot analysis was utilized for the validation of changes in protein expression. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted using the clusterProfiler R package. Compared with the negative control (NC) group, 811 common proteins varied over time, with a cut-off level of two fold change and an adjusted P-value less than 0.05. The bioinformatics analysis revealed that the proteins significantly changed in the B. pseudomallei HNBP001 infection group (Bp group) were enriched in glycometabolism pathways, including glycolysis, fructose and mannose metabolism, pentose phosphate pathway, galactose metabolism, and carbon metabolism. Western blot analysis verified three selected proteins involved in glycometabolism pathways, namely PGM1, PKM, and PGK1 were increase over time post the infection. Furthermore, in vitro functional analysis revealed an increased glucose uptake and decreased ATP production and O-GlcNAcylation in the Bp group compared with control group, suggesting that B. pseudomallei HNBP001 infection induces changes in glycometabolism in RAW264.7 cells. These results indicate that glycometabolism pathways change in RAW264.7 cells post B. pseudomallei HNBP001 infection, providing important insights into the intimate interaction between B. pseudomallei and macrophages.
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- 2022
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12. The triangle relationship between human genome, gut microbiome, and COVID-19: opening of a Pandora’s box
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Jie Tong, Yuran Chen, Mei He, Wenjing Wang, Yiyang Wang, Na Li, and Qianfeng Xia
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COVID-19 ,genome ,gut microbiota ,infectious diseases ,SARS-CoV-2 ,Microbiology ,QR1-502 - Abstract
Since the pandemic started, the coronavirus disease 2019 (COVID-19) has spread worldwide. In patients with COVID-19, the gut microbiome (GM) has been supposed to be closely related to the progress of the disease. The gut microbiota composition and human genetic variation are also connected in COVID-19 patients, assuming a triangular relationship between the genome, GM, and COVID-19. Here, we reviewed the recent developments in the study of the relationship between gut microbiota and COVID-19. The keywords “COVID-19,” “microbiome,” and “genome” were used to search the literature in the PubMed database. We first found that the composition of the GM in COVID-19 patients varies according to the severity of the illness. Most obviously, Candida albicans abnormally increased while the probiotic Bifidobacterium decreased in severe cases of COVID-19. Interestingly, clinical studies have consistently emphasized that the family Lachnospiraceae plays a critical role in patients with COVID-19. Additionally, we have demonstrated the impact of microbiome-related genes on COVID-19. Specially, we focused on angiotensin-converting enzyme 2’s dual functions in SARS-CoV-2 infection and gut microbiota alternation. In summary, these studies showed that the diversity of GMs is closely connected to COVID-19. A triangular relationship exists between COVID-19, the human genome, and the gut flora, suggesting that human genetic variations may offer a chance for a precise diagnosis of COVID-19, and the important relationships between genetic makeup and microbiome regulation may affect the therapy of COVID-19.
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- 2023
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13. Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019
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Lin Liu, Xiujuan Zhao, Xingyong Wu, Sijing Li, Biao Liu, Mamy Jayne Nelly Rajaofera, Yingfei Zeng, Sufang Dong, Zheng Bei, Hua Pei, and Qianfeng Xia
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Mycobacterium tuberculosis ,DNA microarray ,Drug sensitivity test ,Molecular-characteristics ,Drug-resistant rate ,Microbiology ,QR1-502 - Abstract
Abstract Background The emergence of antimicrobial resistance against Mycobacterium tuberculosis (M. tuberculosis) has become the major concern in global tuberculosis control due to its limited therapy options and high mortality. However, the clinical and molecular characteristics of drug-resistant strains vary in different geographical areas. Hainan Island located in southern China, is a high drug-resistant tuberculosis burden area. This study aimed to determine the dynamic changes of drug-resistance patterns and drug-related gene mutation types of M. tuberculosis in Hainan from 2014 to 2019. Results A total of 1484 culture-confirmed M. tuberculosis were included in this study. It was found that the proportions of drug resistance to isoniazid and rifampin were 31.3 and 31.1% respectively. Overall the proportion of multidrug resistant M. tuberculosis was 24.9%. Multivariate logistic regression analysis showed that age and the treatment history were independent influencing factors of drug resistant tuberculosis. The proportions of drug-resistant tuberculosis in retreatment patients were considerably higher than those in new patients. The most common mutation types of isoniazid were Ser315 → Thr (66.3%), and the most common mutation types of rifampin were Ser531 → Leu (41.5%). Conclusions Our data suggests that the prevalence of drug resistant TB remains high in Hainan, and the risks for developing drug resistance with diversified mutation types increased significantly in retreatment patients. These results contribute to the knowledge of the prevalence of drug resistance in Hainan Province and expand the molecular characteristics of drug resistance in China simultaneously.
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- 2021
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14. Genetic differentiation of predominant mosquito species in Hainan province and characterization of mosquito midgut microbiota
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Xun Kang, Biao Liu, Siping Li, and Qianfeng Xia
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mosquito ,mosquito identification ,phylogenetic tree ,16s rrna ,midgut microbiota ,Infectious and parasitic diseases ,RC109-216 - Abstract
Objective: To identify the mosquito species collected in the field of 8 cities/counties of Hainan province, China and to analyze the characteristics of genetic differentiation of the predominant mosquito species. The types, contents and composition characteristics of the midgut microbiota of various mosquito species were explored to provide evidence for the control and prevention of mosquito-borne diseases in Hainan province. Methods: Adult mosquitoes were collected in the field using light traps, human lure or cattle lure methods. Morphology and DNA-barcoding technology (COI gene) were applied to identify the mosquito species. The V3-V4 hypervariable region of microbes 16S rRNA was used for high-throughput sequencing of the midgut microbiota, and SPSS 21.0 and R v3.1.1 software were employed to perform statistical analysis of the sequencing results and the Beta diversity analysis. Results: Aedes (Ae.) albopictus, Armigeres (Ar.) subalbatus and Culex (Cx.) pipiens pallpens were the three predominant species of the mosquito samples. Ae. albopictus was closely related to MK736660 (India) and JQ235749 (Yunnan), while MT541282 (DF, Dongfang) was a relatively independent population. The composition and structure of bacterial communities significantly varied among most of the samples (P
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- 2021
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15. Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat spike protein RBD protects mice against COVID-19
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Kun Xu, Yaling An, Qunlong Li, Weijin Huang, Yuxuan Han, Tianyi Zheng, Fang Fang, Hui Liu, Chuanyu Liu, Ping Gao, Senyu Xu, Xueyuan Liu, Rong Zhang, Xin Zhao, William J. Liu, Yuhai Bi, Youchun Wang, Dongming Zhou, Qinghan Wang, Wenli Hou, Qianfeng Xia, George F. Gao, and Lianpan Dai
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COVID-19 ,SARS-CoV-2 ,vaccine ,adenovirus ,antibody ,immune response ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
A safe and effective vaccine is urgently needed to control the unprecedented COVID-19 pandemic. Four adenovirus-vectored vaccines expressing spike (S) protein have been approved for use. Here, we generated several recombinant chimpanzee adenovirus (AdC7) vaccines expressing S, receptor-binding domain (RBD), or tandem-repeat dimeric RBD (RBD-tr2). We found vaccination via either intramuscular or intranasal route was highly immunogenic in mice to elicit both humoral and cellular immune responses. AdC7-RBD-tr2 showed higher antibody responses compared to either AdC7-S or AdC7-RBD. Intranasal administration of AdC7-RBD-tr2 additionally induced mucosal immunity with neutralizing activity in bronchoalveolar lavage fluid. Either single-dose or two-dose mucosal administration of AdC7-RBD-tr2 protected mice against SARS-CoV-2 challenge, with undetectable subgenomic RNA in lung and relieved lung injury. AdC7-RBD-tr2-elicted sera preserved the neutralizing activity against the circulating variants, especially the Delta variant. These results support AdC7-RBD-tr2 as a promising COVID-19 vaccine candidate.
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- 2021
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16. Prognostic Implications and Immune Infiltration Characteristics of Chromosomal Instability-Related Dysregulated CeRNA in Lung Adenocarcinoma
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Shengnan Guo, Tianhao Li, Dahua Xu, Jiankai Xu, Hong Wang, Jian Li, Xiaoman Bi, Meng Cao, Zhizhou Xu, Qianfeng Xia, Ying Cui, and Kongning Li
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chromosomal instability ,dysregulated ceRNA ,immune microenvironment ,prognosis ,lung adenocarcinoma ,Biology (General) ,QH301-705.5 - Abstract
An accumulating body of research indicates that long-noncoding RNAs (lncRNAs) regulate the target genes and act as competitive endogenous RNAs (ceRNAs) playing an indispensable role in lung adenocarcinoma (LUAD). LUAD is frequently accompanied by the feature of chromosomal instability (CIN); however, CIN-related ceRNAs have not been investigated yet. We systematically analyzed and integrated CIN-related dysregulated ceRNAs characteristics in LUAD samples for the first time. In TCGA LUAD cohort, CIN in tumor samples was significantly higher than that in those of adjacent, and patients with high CIN risk tended to have worse clinical outcomes. We constructed a double-weighted CIN-related dysregulated ceRNA network, in which edge weight and node weight represented the disorder extent of ceRNA and the correlation of RNA expression level and prognosis, respectively. After module mining and analysis, a potential prognostic biomarker composed of 12 RNAs (8 mRNAs and 4 lncRNAs) named CIN-related dysregulated ceRNAs (CRDC) was obtained. The CRDC risk score had a positive relation with clinical stage and CIN, and patients with high CRDC risk scores exhibited poor prognosis. Moreover, CRDC tended to be an independent risk factor with high robustness to overcome the effect of multicollinearity among other explanatory variables for disease-specific survival (DSS) in TCGA and two GEO cohorts. The result of functional analysis indicated that CRDC was involved in multiple cancer progresses, especially immune-related pathways. The patients with lower CRDC risk had higher B cell, T cell CD4+, T cell CD8+, neutrophil, macrophage, and myeloid dendritic cell infiltration than the patients with higher CRDC risk. Meanwhile, patients with lower CRDC risk could get more benefits from immunological therapy. The results suggested that the CRDC could be a potential prognostic biomarker and an immunotherapy predictor for lung adenocarcinoma.
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- 2022
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17. Identification of novel blood-based HCC-specific diagnostic biomarkers for human hepatocellular carcinoma
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Li Yin, Na He, Chuizhe Chen, Nan Zhang, Yingzi Lin, and Qianfeng Xia
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Peripheral blood mononuclear cells (PBMC ,human hepatocellular carcinoma (HCC) ,biomarker ,WGCNA ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Introduction Hepatocellular carcinoma (HCC) ranks fourth in global cancer mortality, accounting for 8.2% of all cancer deaths. Early detection of HCC has a significant impact on clinical outcomes. The aim of this study was to identify blood-based biomarkers which are HCC-specific.Methods Comprehensive gene expression raw data of purified RNA of peripheral blood mononuclear cells (PBMC) was downloaded from GEO and was then analyzed. Differentially expressed genes (DEGs) in HCC were screened and the method of weighted gene co-expression network analysis was applied to identify candidate blood-based biomarkers associated with HCC.Results Three modules closely related to HCC were screened using WGCNA. Nuclear localization signal (NLS)-bearing protein import into nucleus biological process was the most significant enriched physiological process identified by MCODE, and 3 genes (DICER1, GMPS and NCOR1) were selected as biomarkers.Conclusion In our study, three novel blood-based HCC-specific diagnostic biomarkers for human hepatocellular carcinoma were identified. These findings may contribute to the non-invasive detection of early HCC patients.
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- 2019
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18. Explorative analysis of the gene expression profile during liver regeneration of mouse: a microarray-based study
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Li Yin, Yuanyuan Wang, Yingzi Lin, Guoying Yu, and Qianfeng Xia
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Liver regeneration ,maSigPro ,WGCNA ,SAFE ,cytoscape ,cell cycle ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
The liver is an amazing organ due to its powerful regenerative capacity. Although many studies on liver regeneration have been documented, the detailed mechanisms remain unclear. Two-third partial hepatectomy (PH) in rodents plays a crucial role in the study of liver regeneration. In this study, the time series data of gene expression during liver regeneration in mouse were analyzed using the gene set numbered GSE6998 in GEO. A variety of bioinformatics methods, including masigPro, Weighted Gene Co-expression Network Analysis (WGCNA), spatial analysis of functional enrichment (SAFE) and ingenuity canonical pathway analysis (IPA) were used to identify and compare the significantly changed pathways, potential upstream regulators and key genes during liver regeneration. Our study showed that liver regeneration in the mouse is a coordinated process, which cell-cycle-related progress are at the centre of the interaction network involved in liver regeneration. Several candidate upstream regulators including PPARA, NFE2L2, MAD1 and CNR1 and some key genes such as Cdk1, Plk1, Cdc20, Aurka, Racgap1, Cenpa, Rrm1, Rrm2 were identified. In conclusion, these findings could contribute to revealing the molecular mechanism of liver regeneration after PH, which could provide new ideas and treatment methods for regenerative medicine, oncological drug development and oncological treatment.
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- 2019
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19. The Origin and Molecular Epidemiology of Dengue Fever in Hainan Province, China, 2019
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Lin Liu, Tao Wu, Biao Liu, Rajaofera Mamy Jayne Nelly, Yumei Fu, Xun Kang, Chuizhe Chen, Zenyan Huang, Biao Wu, Jiao Wang, Zhongyi Zhu, Jinmin Ma, Ming Liu, Yanru Zhang, Chuanyu Bao, Feng Lin, Weijun Chen, and Qianfeng Xia
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dengue fever ,molecular-characteristics ,bayesian phylogenies ,hainan province ,epidemiological ,Microbiology ,QR1-502 - Abstract
There was an outbreak of Dengue fever on September 5, 2019, in Hainan Province, which has not been endemic for 28 years. We aim to describe the clinical and epidemiological features of the 2019 outbreak in Hainan Province and identify the cause. All type 1 Dengue fever cases that occurred in this outbreak of Hainan exhibited mild clinical symptoms. The epidemiological investigations indicate that the outbreak might originate from workers in the Xiuying area, Haikou City, form a concentrated outbreak, and then spread out. Bayesian phylogenies results and epidemiological data were used to infer a likely series of events for the dengue virus’s potential spread and trace the possible sources. The strains’ sequences were close to a sequence from the nearby Guangdong province, supporting the hypothesis that the dengue virus was imported from Guangdong province and then spread across Hainan province. Furthermore, it is interesting that two other strains didn’t group with this cluster, suggesting that additional introduction pathways might exist. The study indicated that the dengue fever epidemic presented two important modes in Hainan. Firstly, epidemics prevalence was caused by imported cases, and then endogenous epidemics broke out in the natural epidemic focus.
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- 2021
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20. Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China
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Xun Kang, Yanhong Wang, Siping Li, Xiaomei Sun, Xiangyang Lu, Mamy Jayne Nelly Rajaofera, Yajun Lu, Le Kang, Aihua Zheng, Zhen Zou, and Qianfeng Xia
- Subjects
Hainan ,mosquitoes ,midgut microbiome ,bacterial community ,16S rRNA ,Microbiology ,QR1-502 - Abstract
The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits and how the microbiome influences community structure. We analyzed the composition of 501 midgut bacterial communities from field-collected adult female mosquitoes, including Aedes albopictus, Aedes galloisi, Culex pallidothorax, Culex pipiens, Culex gelidus, and Armigeres subalbatus, across eight habitats using the HiSeq 4000 system and the V3−V4 hyper-variable region of 16S rRNA gene. After quality filtering and rarefaction, a total of 1421 operational taxonomic units, belonging to 29 phyla, 44 families, and 43 genera were identified. Proteobacteria (75.67%) were the most common phylum, followed by Firmicutes (10.38%), Bacteroidetes (6.87%), Thermi (4.60%), and Actinobacteria (1.58%). The genera Rickettsiaceae (33.00%), Enterobacteriaceae (20.27%), Enterococcaceae (7.49%), Aeromonadaceae (7.00%), Thermaceae (4.52%), and Moraxellaceae (4.31%) were dominant in the samples analyzed and accounted for 76.59% of the total genera. We characterized the midgut bacterial communities of six mosquito species in Hainan province, China. The gut bacterial communities were different in composition and abundance, among locations, for all mosquito species. There were significant differences in the gut microbial composition between some species and substantial variation in the gut microbiota between individuals of the same mosquito species. There was a marked variation in different mosquito gut microbiota within the same location. These results might be useful in the identification of microbial communities that could be exploited for disease control.
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- 2020
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21. Mosquito Diversity and Population Genetic Structure of Six Mosquito Species From Hainan Island
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Siping Li, Feng Jiang, Hong Lu, Xun Kang, Yanhong Wang, Zhen Zou, Dan Wen, Aihua Zheng, Chunxiang Liu, Qiyong Liu, Le Kang, Qianfeng Xia, and Feng Cui
- Subjects
Aedes ,Armigeres ,Culex ,Mansonia ,Anopheles ,cytochrome c oxidase subunit I ,Genetics ,QH426-470 - Abstract
Hainan is a tropical island in southern China with abundant mosquito species, putting Hainan at risk of mosquito-borne virus disease outbreaks. The population genetic diversity of most mosquito species on Hainan Island remains elusive. In this study, we report the diversity of mosquito species and the genetic diversity of the predominant species on Hainan. Field populations of adults or larvae were collected from 12 regions of Hainan Island in 2018 and 2019. A fragment of the mitochondrial cytochrome c oxidase subunit I (coxI) gene was sequenced from 1,228 mosquito samples and used for species identification and genetic diversity analysis. Twenty-three known mosquito species from the genera Aedes, Armigeres, Culex, Mansonia, and Anopheles and nine unconfirmed mosquito species were identified. Aedes albopictus, Armigeres subalbatus, and Culex pipiens quinquefasciatus were the most prevalent mosquito species on Hainan. The regions north of Danzhou, Tunchang, and Qionghai exhibited high mosquito diversity (26 species). The order of the total haplotype diversity and nucleotide diversity of the populations from high to low was as follows: Culex tritaeniorhynchus, Ar. subalbatus, Culex pallidothorax, Culex gelidus, Ae. albopictus, and C. p. quinquefasciatus. Tajima’s D and Fu’s Fs tests showed that Ae. albopictus, C. p. quinquefasciatus, C. tritaeniorhynchus, and C. gelidus had experienced population expansion, while the Ar. subalbatus and C. pallidothorax populations were in genetic equilibrium. Significant genetic differentiation existed in the overall populations of Ae. albopictus, Ar. subalbatus, C. p. quinquefasciatus, and C. pallidothorax. The Ae. albopictus populations on Hainan were characterized by frequent gene exchange with populations from Guangdong and four other tropical countries, raising the risk of viral disease outbreaks in these regions. Two subgroups were reported in the Ar. subalbatus populations for the first time. Our findings may have important implications for vector control on Hainan Island.
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- 2020
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22. Pediatric suppurative parotitis caused by Burkholderia pseudomallei
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Zengzhen Fu, Yingzi Lin, Qiang Wu, and Qianfeng Xia
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Melioidosis ,Parotitis ,Burkholderia pseudomallei ,Pediatrics ,Arctic medicine. Tropical medicine ,RC955-962 ,Toxicology. Poisons ,RA1190-1270 ,Zoology ,QL1-991 - Abstract
Abstract Background: Suppurative parotitis caused by Burkholderia pseudomallei has been rarely found outside endemic areas. Case presentation: Herein, we report the recovery of Burkholderia pseudomallei from the pus of a suppurative parotitis observed in a 12-year-old boy who lived in Hainan province, China. Specimens of necrotic tissue were collected and sections were stained with hematoxylin and eosin. Pus sample was also collected for bacteriological examination. The suppurative inflammation was observed in the necrotic tissue section and Burkholderia pseudomallei were detected in the sample. Conclusion: In this adolescent, Burkholderia pseudomallei infection was present in the parotid, which consists of the first report of this bacterium in a parotitis case acquired in China.
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- 2016
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23. Endemic Melioidosis in Southern China: Past and Present
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Xiao Zheng, Qianfeng Xia, Lianxu Xia, and Wei Li
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melioidosis ,Burkholderia pseudomallei ,epidemiology ,China ,Medicine - Abstract
Melioidosis is a severe tropical infectious disease caused by the soil-dwelling bacterium Burkholderia pseudomallei, predominantly endemic to Southeast Asia and northern Australia. Between the 1970s and the 1990s, the presence of B. pseudomallei causing melioidosis in humans and other animals was demonstrated in four coastal provinces in southern China: Hainan, Guangdong, Guangxi, and Fujian, although indigenous cases were rare and the disease failed to raise concern amongst local and national health authorities. In recent years, there has been a rise in the number of melioidosis cases witnessed in the region, particularly in Hainan. Meanwhile, although China has established and maintained an effective communicable disease surveillance system, it has not yet been utilized for melioidosis. Thus, the overall incidence, social burden and epidemiological features of the disease in China remain unclear. In this context, we present a comprehensive overview of both historical and current information on melioidosis in Southern China, highlighting the re-emergence of the disease in Hainan. Surveillance and management strategies for melioidosis should be promoted in mainland China, and more research should be conducted to provide further insights into the present situation.
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- 2019
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24. Key molecules regulating the blood meals of Rhipicephalus sanguineus (Acari: Ixodidae) revealed by transcriptomics.
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Yajun Lu, Yijia Xu, Chenghang Yu, Shi Cheng, Qianfeng Xia, and Zheng Bin
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BROWN dog tick ,TRANSFER RNA ,GLYCOPROTEINS ,EPITHELIUM ,GENE ontology - Abstract
Rhipicephalus sanguineus, a repulsive obligate blood feeder, is a three-host tick inflicting tremendous damage. Blood-sucking initiates tick-pathogen-host interactions along with alterations in the expression levels of numerous bioactive ingredients. Key molecules regulating blood meals were identified using the transcriptomic approach. A total number of 744 transcripts showed statistically significantly differential expression including 309 significantly upregulated transcripts and 435 significantly downregulated transcripts in semiengorged female ticks compared to unfed ticks, all collected in 2021. The top 10 differentially upregulated transcripts with explicit functional annotations included turripeptide OL55-like protein, valine tRNA ligase-like protein and ice-structuring glycoprotein-like protein. The top 10 differentially down-regulated transcripts were uncharacterized proteins. Gene Ontology (GO) enrichment analysis revealed four associated terms in the cellular component category and 16 in the molecular function category among the top 20 terms. Differentially expressed genes (DEGs) were enriched in GO terms ID 0000323 (lytic vacuole) and ID 0005773 (vacuole). The top 20 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included metabolism, cellular processes, organismal systems and human diseases. The DEGs were enriched in the KEGG term ID: ko-04142 (lysosome pathway) associated with intracellular digestion in the tick midgut epithelium. Molecular markers annotated via comparative transcriptomic profiling were expected to be candidate markers for the purpose of tick control. [ABSTRACT FROM AUTHOR]
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- 2024
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25. Infection and transovarial transmission of severe fever with thrombocytopenia syndrome virus in Rhipicephalus sanguineus in Hainan Island, China
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Chuanfei YUAN, Yajun LU, Jinqian LI, Chen CHEN, Yanhong WANG, Aihua ZHENG, Zhen ZOU, and Qianfeng XIA
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Animal Science and Zoology - Published
- 2023
26. Screening core genes and signaling pathways after SFTSV infection by integrated transcriptome profiling analysis
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Huimin Fu, Yanhong Wang, Chuanfei Yuan, Yuhang Zhang, Aihua Zheng, Zhen Zou, and Qianfeng Xia
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Cancer Research ,Infectious Diseases ,Virology - Published
- 2023
27. Delayed ventricular septal rupture complicated with ventricular aneurysm in a case of myocardial infarction with non-obstructive coronary arteries
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Si Wang, Xu Huang, Qianfeng Xiao, Ying Xu, and Xin Wei
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Ventricular septal rupture ,Ventricular aneurysm ,Myocardial infarction ,Non-obstructive ,MINOCA ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Myocardial infarction with non-obstructive coronary arteries (MINOCA) is a rare cause of heart attack, which may not receive sufficient attention from patients during post-discharge treatment, especially among those with normal coronary angiography results. Case presentation We present the case of a 65-year-old woman who was readmitted to the hospital with ventricular septal rupture (VSR) complicated by ventricular aneurysm, occurring 2 weeks after myocardial infarction. During the initial admission, coronary angiography revealed normal coronary arteries, leading to a diagnosis of MINOCA. Epicardial coronary vasospasm or coronary embolism was considered as potential causes; however, the patient did not adhere to standardized treatment upon initial discharge. The delayed VSR led to a decline in cardiac function but did not result in severe hemodynamic impairment. Following correction of heart failure with medications, the patient underwent percutaneous VSR repair 19 days after diagnosis and was discharged with a favorable recovery. Conclusions The occurrence of delayed VSR complicated with ventricular aneurysm in patients with MINOCA is rare, highlighting the possibility of serious complications in MINOCA cases. Both cardioprotective therapies and cause-targeted therapies are essential in the management of patients with MINOCA.
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- 2024
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28. Septicemic Melioidosis Detection Using Support Vector Machine with Five Immune Cell Types
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Ke Xu, Fang Lian, Yunfan Quan, Jun Liu, Li Yin, Xuexia Li, Shen Tian, Hua Pei, and Qianfeng Xia
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Medicine (General) ,Support Vector Machine ,Article Subject ,Biochemistry (medical) ,Clinical Biochemistry ,General Medicine ,Sensitivity and Specificity ,R5-920 ,Melioidosis ,Case-Control Studies ,Sepsis ,Genetics ,Humans ,Molecular Biology ,Biomarkers ,Research Article - Abstract
Melioidosis, caused by Burkholderia pseudomallei (B. pseudomallei), predominantly occurs in the tropical regions. Of various types of melioidosis, septicemic melioidosis is the most lethal one with a mortality rate of 40%. Early detection of the disease is paramount for the better chances of cure. In this study, we developed a novel approach for septicemic melioidosis detection, using a machine learning technique—support vector machine (SVM). Several SVM models were built, and 19 features characterized by the corresponding immune cell types were generated by Cell type Identification Estimating Relative Subsets Of RNA Transcripts (CIBERSORT). Using these features, we trained a binomial SVM model on the training set and evaluated it on the independent testing set. Our findings indicated that this model performed well with means of sensitivity and specificity up to 0.962 and 0.979, respectively. Meanwhile, the receiver operating characteristic (ROC) curve analysis gave area under curves (AUCs) ranging from 0.952 to 1.000. Furthermore, we found that a concise SVM model, built upon a combination of CD8+ T cells, resting CD4+ memory T cells, monocytes, M2 macrophages, and activated mast cells, worked perfectly on the detection of septicemic melioidosis. Our data showed that its mean of sensitivity was up to 0.976 while that of specificity up to 0.993. In addition, the ROC curve analysis gave AUC close to 1.000. Taken together, this SVM model is a robust classification tool and may serve as a complementary diagnostic technique to septicemic melioidosis.
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- 2021
29. AuNPs@MoSe
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Yao, Shi, Jie, Wu, Wenxin, Wu, Nini, Luo, Hao, Huang, Yuhui, Chen, Jun, Sun, Qian, Yu, Hang, Ao, Qiqi, Xu, Xiaotian, Wu, Qianfeng, Xia, and Huangxian, Ju
- Subjects
Immunoassay ,Luminescent Measurements ,Metal Nanoparticles ,Reproducibility of Results ,Gold ,Biosensing Techniques ,Electrochemical Techniques ,DNA Methylation ,Silicon Dioxide - Abstract
Electrocheluminescence analysis amplified by coreaction accelerators has experienced breakthrough in ultrasensitive detection of biomarkers. Herein, a highly efficient coreaction accelerator, two-dimensional layered MoSe
- Published
- 2022
30. The efficacy and safety of remdesivir and its combination with other drug for the treatment of COVID-19: a systematic review and meta-analysis
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Chuizhe Chen, Junde Fang, Shu Chen, Mamy Jayne Nelly Rajaofera, Xuemiao Li, Bo Wang, and Qianfeng Xia
- Abstract
Background Remdesivir was considered to be a specific drug for Corona Virus Disease 2019. This systematic review aims to evaluate remdesivir monotherapy and combination therapy related clinical efficacy and risk. Research design and methods PubMed, Embase, SCIE, Cochrane Library, and American Clinical trial Center were searched up to 1 April 2022. We included randomized controlled trials (RCTs) comparing Remdesivir monotherapy with control drugs, or comparing different combination therapy. Results 11 RCTs and 32 observational studies were included in analysis. In the main outcome, remdesivir use reduced mortality in patients with severe COVID-19 and improve recovery in patients. In other clinical outcomes, remdesivir use was associated with improved clinical status. In safety outcomes, remdesivir use did not cause liver or kidney damage. Compared with remdesivir alone, remdesivir combined with other drugs-steroids, favipiravir, and convalescent plasma- had no effect on mortality. In addition, remdesivir combined with tocilizumab may increase mortality. Conclusion Results of the systematic review showed that remdesivir was positive in COVID-19, especially patients with severe COVID-19. The more effective treatment of COVID-19 with other drugs combined with remdesivir is urgent and challenging research. Trial registration number PROSPERO registration number: CRD42022322859.
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- 2022
31. Protective Zika vaccines engineered to eliminate enhancement of dengue infection via immunodominance switch
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Qihui Wang, Yuxuan Han, Jinghua Yan, Jinhe Li, Huabing Yang, Ping Gao, Jianxun Qi, Tianyi Zheng, Jian Song, Kun Xu, George F. Gao, Yan Chai, Xuancheng Lu, Kefang Liu, Qianfeng Xia, Qingrui Huang, and Lianpan Dai
- Subjects
Immunogen ,Immunology ,Immunodominance ,Dengue virus ,Biology ,medicine.disease ,medicine.disease_cause ,biology.organism_classification ,Virology ,Epitope ,Dengue fever ,Zika virus ,Vaccination ,Immunity ,medicine ,Immunology and Allergy - Abstract
Antibody-dependent enhancement (ADE) is an important safety concern for vaccine development against dengue virus (DENV) and its antigenically related Zika virus (ZIKV) because vaccine may prime deleterious antibodies to enhance natural infections. Cross-reactive antibodies targeting the conserved fusion loop epitope (FLE) are known as the main sources of ADE. We design ZIKV immunogens engineered to change the FLE conformation but preserve neutralizing epitopes. Single vaccination conferred sterilizing immunity against ZIKV without ADE of DENV-serotype 1-4 infections and abrogated maternal-neonatal transmission in mice. Unlike the wild-type-based vaccine inducing predominately cross-reactive ADE-prone antibodies, B cell profiling revealed that the engineered vaccines switched immunodominance to dispersed patterns without DENV enhancement. The crystal structure of the engineered immunogen showed the dimeric conformation of the envelope protein with FLE disruption. We provide vaccine candidates that will prevent both ZIKV infection and infection-/vaccination-induced DENV ADE.
- Published
- 2021
32. Atomic structure and electronic properties of the intercalated Pb atoms underneath a graphene layer
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Fei Ma, Yan Li, Tingwei Hu, Haitao Gao, Ke-Wei Xu, Xiangtai Liu, Qianfeng Xia, and Dong Yang
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Materials science ,Graphene ,Intercalation (chemistry) ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,Crystal ,Crystallography ,Zigzag ,law ,General Materials Science ,Density functional theory ,Scanning tunneling microscope ,0210 nano-technology ,Spectroscopy ,Layer (electronics) - Abstract
Determining atomic structure of intercalated metallic layer under graphene is a hot-topic due to the potential applications in electronic engineering. In this study, Pb-intercalated graphene (PbG) is fabricated on SiC substrate and the atomic structure and electronic properties of the intercalated Pb atoms underneath graphene are investigated by scanning tunneling microscopy/spectroscopy (STM/STS). As the crystal facet of intercalated Pb atoms can be arranged in (111) (unit cell, 0.35 nm × 0.35 nm) and (110) (unit cell, 0.61 nm × 0.35 nm), mottled moire pattern (period, 2.5 nm) and striated moire pattern (period, 2.1 nm) are formed. Atomic arrangement of the intercalated Pb is sensitive to tip scanning resulting in the conversion of the two moire patterns. Nevertheless, the crystal orientation of moire patterns is always aligned with zigzag direction of graphene. As revealed by STS, PbG performs the similar electronic states to those of free-standing graphene due to the shielding effects of Pb intercalation. The crystallographic relationship between graphene and Pb atoms is also confirmed by density functional theory (DFT) calculations. The study provides crucial for understanding the structure of intercalated Pb atoms underneath a graphene layer, which may enable the development of new 2D materials capped by graphene.
- Published
- 2021
33. Electrochemiluminescent (ECL) biosensor for Burkholderia pseudomallei based on cobalt-doped MOF decorated with gold nanoparticles and N-(4-aminobutyl)-N-(ethylisoluminol)
- Author
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Yuexin, Wang, Rui, Chen, Bo, Shen, Cai, Li, Junman, Chen, Yanshuang, Wang, Shen, Tian, Xuemiao, Li, Nini, Luo, Rui, Liu, Shijia, Ding, Chuanlong, Zhu, and Qianfeng, Xia
- Subjects
Burkholderia pseudomallei ,Luminescent Measurements ,Metal Nanoparticles ,Luminol ,Biosensing Techniques ,Cobalt ,Electrochemical Techniques ,Gold ,Metal-Organic Frameworks ,Analytical Chemistry - Abstract
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal-organic frameworks (Co-MOF), gold nanoparticles (AuNP), and N-(4-aminobutyl)-N-(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay of Burkholderia pseudomallei with the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination of B. pseudomallei in serum sample was as low as 9.0 CFU mL
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- 2022
34. Burkholderia pseudomallei infection manifests as mediastinal/hilar lymphadenopathy: A case report
- Author
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Xiang-Dong Zhou, Qi Li, Qiong-Fang Sun, and Qianfeng Xia
- Subjects
medicine.medical_specialty ,Melioidosis ,lcsh:Arctic medicine. Tropical medicine ,biology ,Mediastinal lymphadenopathy ,Burkholderia pseudomallei ,business.industry ,lcsh:RC955-962 ,Sulfamethoxazole ,endobronchial ultrasound-transbronchial needle aspiration ,lymphnode ,mediastinum ,melioidosis ,Ceftazidime ,Mediastinum ,General Medicine ,medicine.disease ,biology.organism_classification ,Trimethoprim ,Surgery ,Pneumonia ,medicine.anatomical_structure ,medicine ,business ,medicine.drug - Abstract
Rationale: This case report presents the diagnosis and etiology of hilar/mediastinal lymphadenopathy in a male patient. Patient concerns: A 49-year-old man presented with fever and dyspnea after physical exertion. Diagnosis: The patient was diagnosed with melioidosis by cultivation of lymph node aspirate on blood agar using the VITEK 2 compact system. Interventions: The patient was treated with ceftazidime intravenously, combined with trimethoprim/sulfamethoxazole orally for 1 week. Once the patient was discharged, he began a 12-week course of trimethoprim/sulfamethoxazole. Outcomes: The patient recovered after treatment with ceftazidime and trimethoprim/sulfamethoxazole. Conclusions: Melioidosis is an infectious disease that mainly occurs in tropical regions. It can cause severe sepsis and pneumonia, and the infection in some patients may become chronic. Endobronchial ultrasound-transbronchial needle aspiration is a useful technique in the diagnosis of patients with hilar/mediastinal lymphadenopathy.
- Published
- 2021
35. The structure and mechanism of large-scale indium-intercalated graphene transferred from SiC buffer layer
- Author
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Wei Hu, Fei Ma, Tingwei Hu, Dong Yang, Qianfeng Xia, and Ke-Wei Xu
- Subjects
Materials science ,Fabrication ,Annealing (metallurgy) ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Buffer (optical fiber) ,law.invention ,symbols.namesake ,X-ray photoelectron spectroscopy ,law ,General Materials Science ,business.industry ,Graphene ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,symbols ,Optoelectronics ,Scanning tunneling microscope ,0210 nano-technology ,business ,Raman spectroscopy ,Indium - Abstract
It is reported that indium-coated SiC buffer layer can be directly transferred into graphene layer during low temperature annealing, forming as indium-intercalated graphene (InG). Atomic structures of InG as well as transformation mechanism are investigated by scanning tunneling microscopy (STM), Raman and X-ray photoelectron spectroscopy (XPS). InG on top of one-layered intercalated indium (called O-InG) exhibits Moire patterns with period of 3.2 nm, and the atomic arrangement of indium atoms is detected. While, no Moire patterns are observed on InG intercalated by two-layer of indium atoms (called T-InG). Both the two InG regions bind perfectly with the already existing epitaxial graphene (EG), exhibiting as an unbroken sheet of quasi-free-standing graphene layer. This finding provides a simple strategy and benefits to industrial fabrication of large-scale InG with regular SiC steps.
- Published
- 2021
36. A ratiometric fluorescent biosensor for rapid detection of Burkholderia pseudomallei by dual CRISPR/Cas12a trans-cleavage assisted signal enhancement
- Author
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Shoujia Lin, Yingzi Lin, Jie Wu, Guangming Li, Xiaotian Wu, Nini Luo, Wenting Li, Chuanlong Zhu, Rui Liu, Qiqi Xu, Qianfeng Xia, and Huangxian Ju
- Subjects
Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
37. An enzyme-free electrochemical biosensor based on NiCoP@PtCu nanozyme and multi-MNAzyme junctions for ultrasensitive Uracil-DNA glycosylase detection
- Author
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Guozhen Tian, Wenting Li, Biao Liu, Meifang Xiao, and Qianfeng Xia
- Subjects
Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
38. Target-Catalyzed Assembly of Pyrene-Labeled Hairpins for Exponentially Amplified Biosensing
- Author
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Qiang Wu, Bin Qiao, Qianfeng Xia, Hua Pei, Huangxian Ju, Jie Wu, and Zhengqing He
- Subjects
Chemistry ,Biochemistry (medical) ,technology, industry, and agriculture ,Biomedical Engineering ,General Chemistry ,Combinatorial chemistry ,Isothermal process ,Catalysis ,Biomaterials ,γ cyclodextrin ,chemistry.chemical_compound ,Exponential growth ,Nucleic acid ,Pyrene ,Biosensor ,Signal amplification - Abstract
Rapid and sensitive detection of nucleic acids is vital for disease diagnosis. This work designed an enzyme-free isothermal strategy for rapid exponential signal amplification through target-triggered catalytic hairpin assembly (CHA) to induce the spatially sensitive fluorescent signal of the pyrene excimer. Functionally, this system consisted of three pyrene labelled hairpins (H1, H2, and H3) and one catalyst DNA C. In the presence of C, the CHA was activated to generate intermediate I, which contained a single-stranded region identical to the C sequence for initiating the second cycle of CHA to obtain 2I and thus achieved the exponential formation of I along with the switching of pyrene excimer. The fluorescent signal of the pyrene excimer could be further enhanced via the inclusion of γ-cyclodextrin and showed a linear increase upon increasing logarithm of C concentration. Through the introduction of a helping hairpin H4-containing C sequence and a region specific to the target, this strategy could be extended to realize the quick and sensitive detection of different analytes. Using dengue virus RNA as an analyte model, the proposed fluorescent method showed a linear range from 0.1 to 50 nM with a limit of detection of 0.048 nM at 3σ and good selectivity. The excellent performance and convenient operation demonstrated its promising application in clinical disease diagnosis.
- Published
- 2022
39. Surface Plasma Resonance Biosensing of Phosphorylated Proteins Via Ph-Adjusted Specific Binding of Phosphate Residues with Uio-66
- Author
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Nini Luo, Yue Shu, Chuanlong Zhu, Xiaoxue Cheng, Lixue Chen, Jiang Wang, Rui Liu, Huangxian Ju, Wei Cheng, and Qianfeng Xia
- Subjects
History ,Polymers and Plastics ,General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
40. A Novel Crispr/Cas14a-Based Electrochemical Biosensor for Ultrasensitive Detection of Burkholderia Pseudomallei with Ptpd@Pcn-224 Nanoenzymes for Signal Amplification
- Author
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Cai Li, Changjin Liu, Rui Liu, Yuexin Wang, Anyang Li, Shen Tian, Wei Cheng, Shijia Ding, Wenting Li, Min Zhao, and Qianfeng Xia
- Subjects
History ,Polymers and Plastics ,Electrochemistry ,Biomedical Engineering ,Biophysics ,General Medicine ,Business and International Management ,Industrial and Manufacturing Engineering ,Biotechnology - Published
- 2022
41. Assessment of Medical Laboratory Undergraduates Training in Different School Year Systems
- Author
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Jun Yang, Qianfeng Xia, and Yajun Lu
- Subjects
China ,020205 medical informatics ,Hospital setting ,Clinical Biochemistry ,Medical laboratory ,02 engineering and technology ,03 medical and health sciences ,0302 clinical medicine ,Medical Laboratory Personnel ,0202 electrical engineering, electronic engineering, information engineering ,Humans ,Training plan ,030212 general & internal medicine ,Medical education ,business.industry ,Biochemistry (medical) ,Subject (documents) ,General education ,Test (assessment) ,Coursework ,Cohort ,Curriculum ,Educational Measurement ,Psychology ,business ,Education, Medical, Undergraduate - Abstract
Objective To assess the effect of the change from the 5-year system of coursework to a 4-year system on the achievements of medical laboratory undergraduates. Methods We analyzed and then compared the topics of training and the test scores among all subject individuals in the 5-year vs the 4-year undergraduate program. Results Five-year-program undergraduates and 4-year-program undergraduates were required to complete 50 courses. The average weekly education time in the 4-year program (27.05 lesson-hours/week) was greater than that in the 5-year program (22.99 lesson-hours/week). The proportion of clinical practice in the hospital setting in the 4-year program (26.8%) was higher than that in the 5-year program (24.5%). The average, excellent, and good scores among 4-year-program undergraduates in general education courses, professional basic courses, professional courses, all courses, and common courses were lower than those scores among the 5-year-program undergraduates. Conclusions The 5-year undergraduate program should be adapted to help boost the achievements and practical skills among its students, in helping them adapt quickly to the new, 4-year training plan (which presented a serious challenge in our cohort).
- Published
- 2020
42. An anchored monopodial DNA walker triggered by proximity hybridization for amplified amperometric biosensing of nucleic acid and protein
- Author
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Qiang Wu, Li Yin, Qianfeng Xia, Jinbo Liu, Jie Wu, Yi Man, Huangxian Ju, and Hua Pei
- Subjects
Aptamer ,Immobilized Nucleic Acids ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Limit of Detection ,Environmental Chemistry ,Electrodes ,Spectroscopy ,Detection limit ,Base Sequence ,Chemistry ,Inverted Repeat Sequences ,010401 analytical chemistry ,Thrombin ,Nucleic Acid Hybridization ,DNA walker ,DNA ,Electrochemical Techniques ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,Amperometry ,0104 chemical sciences ,Electrochemical gas sensor ,Nucleic acid ,Biophysics ,0210 nano-technology ,Biosensor - Abstract
This work designed an anchored monopodial DNA walker to amplify amperometric biosensing signal for sensitive detection of nucleic acid and protein. The biosensing surface was constructed by self-assembling hairpin DNA1 (H1) and small amount of P1-W (probe DNA1 hybridized with walking DNA) on a gold electrode. In the presence of target molecule, the walker could be triggered by the surface proximity hybridization product of P1, target and P2 to induce the cyclic hybridization of H1 with ferrocene modified hairpin DNA2 (H2-Fc), which took electroactive Fc to the electrode surface for amplified amperometric detection of the target. By linking P1 and P2 with dual specific DNA strands, aptamers or antibodies to recognize the target for proximity hybridization of P1 and P2, the walker amplified amperometric strategy could be used for highly sensitive biosensing of different targets. Using DNA and thrombin as the target models, the proposed biosensing methods achieved the linear range from 0.2 pM to 2 nM with a detection limit of 0.11 pM and 1.0 pM to 10 nM with a detection limit of 0.61 pM, respectively. The specific recognition process endowed the strategy with high selectivity and potential applications.
- Published
- 2020
43. Antibacterial activity of bacillomycin D-like compounds isolated from Bacillus amyloliquefaciens HAB-2 against Burkholderia pseudomallei
- Author
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Li Yin, Xin Chen, Mamy Jayne Nelly Rajaofera, Qing-hui Sun, Weiguo Miao, Nan Zhang, Chen-Chu Li, Qianfeng Xia, Lin Liu, Xun Kang, Chuizhe Chen, Pengfei Jin, and Na He
- Subjects
lcsh:Arctic medicine. Tropical medicine ,biology ,Bacillus amyloliquefaciens ,Burkholderia pseudomallei ,lcsh:RC955-962 ,bacillomycin dc ,bioactive compound ,burkholderia pseudomallei ,biology.organism_classification ,Antimicrobial ,bacterial infections and mycoses ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Microbiology ,law.invention ,Bacillomycin ,chemistry.chemical_compound ,Minimum inhibitory concentration ,chemistry ,lcsh:Biology (General) ,law ,bacteria ,Agar diffusion test ,Antibacterial activity ,lcsh:QH301-705.5 ,Polymerase chain reaction - Abstract
Objective: To investigate the inhibitory effect on Burkholderia pseudomallei (B. pseudomallei) strain HNBP001 of a bacillomycin D-like cyclic lipopeptide compound named bacillomycin DC isolated from Bacillus amyloliquefaciens HAB-2. Methods: The antibacterial effect of bacillomycin DC on B. pseudomallei was determined using the disk diffusion method. The minimum inhibitory concentrations were evaluated by microdilution assay. In addition, transmission electron microscopy was performed and quantitative real-time polymerase chain reaction assay was carried out to determine the expression of MexB, OprD2, and qnrS genes. Results: Bacillomycin DC produced an inhibition zone against B. pseudomallei with minimum inhibitory concentration values of 12.5 μg/mL 24 h after treatment and 50 μg/mL at 48 and 72 h. Transmission electron microscopy showed that bacillomycin DC resulted in roughening cell surface and cell membrane damage. Quantitative real-time polymerase chain reaction analysis showed low expression of MexB, OprD2 and qnrS genes. Conclusions: Bacillomycin DC inhibits the growth of B. pseudomallei and can be a new candidate for antimicrobial agents of B. pseudomallei.
- Published
- 2020
44. Multifunctional MOF-based Electrochemiluminescent Nanocubes for an Ultrasensitive Biosensing Strategy of B. Pseudomallei Determination Coupled With 3D Magnetic Walking Nanomachine
- Author
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Yuexin Wang, Bo Shen, Cai Li, Haiping Wu, Yanshuang Wang, Shen Tian, Xuemiao Li, Nini Luo, Rui Liu, Xinmin Li, Junman Chen, Wei Cheng, Shijia Ding, Chuanlong Zhu, and Qianfeng Xia
- Abstract
Burkholderia pseudomallei (B. pseudomallei) can cause melioidosis that is usually fatal. A reliable and rapid detection method is greatly needed for disease surveillance and diagnosis. Herein, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed for accurate determination of B. pseudomallei coupled with multifunctional Au@Co-MOF@ABEI nanocubes and 3D magnetic walking nanomachine. The synthesized nanocubes could not only immobilize enormous ABEI but exhibit superior peroxidase-like activity to decompose H2O2 to produce plentiful reactive oxygen species (ROSs) that could further react with ABEI, so that the enhanced ECL signals were achieved. Meanwhile, the target-activated walking nanomachine was efficiently driven by Exonuclease III (Exo III) for further improving the sensitivity of the biosensor. As a result, the fabricated ECL biosensor could detect pathogenic gene down to 60.3 aM with a linear range from 100.0 aM to 100.0 pM. Moreover, the biosensing platform successfully achieved the determination of B. pseudomallei down to 9.0 CFU mL−1 in serum samples. This work exhibited an ultrasensitive and specific performance for B. pseudomallei detection, which would become a versatile tool in the early diagnosis and treatment of melioidosis.
- Published
- 2021
45. Proteomics reveals the hemolymph components of partially fed Hyalomma asiaticum ticks
- Author
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Chuanfei Yuan, Qingtai Yang, Jia Wu, Yun Peng, Yufeng Li, Shirui Xiong, Jinlin Zhou, Manli Wang, Zhihong Hu, Zhen Zou, and Qianfeng Xia
- Subjects
Infectious Diseases ,Insect Science ,Parasitology ,Microbiology - Abstract
Hemolymph infection facilitates pathogen invasion of internal tick tissues. However, the overall protein composition of the hemolymph has not been analyzed for any tick species. Here, a gel based liquid chromatography tandem mass spectrometry method was used to characterize the hemolymph proteome of Hyalomma asiaticum females during blood feeding. A total of 311 proteins were identified. Hemelipoglyco-carrier proteins, apolipophorin-like proteins, and intracellular proteins were the most abundant proteins. Thirteen immunity-related proteins were identified, including peptidoglycan recognition protein (PGRP), Thioester-containing proteins (TEPs), clip‑serine proteinases, serpins and Dome. The presence of hemocytin, proclotting enzyme homologs, serpins, TEPs, factor D-like protein and the lack of coagulin, hemocyanin, and prophenoloxidase suggest ticks may possess a unique coagulation system, which is largely different from that of insects. Taken together, the study revealed the constitution, level, and possible functions of global hemolymph proteins in H. asiaticum and could facilitate the discovery of new targets for control of tick-borne pathogens.
- Published
- 2022
46. A sensitive electrochemical method for rapid detection of dengue virus by CRISPR/Cas13a-assisted catalytic hairpin assembly
- Author
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Yingzi Lin, Jiaojiao Wang, Jie Wu, Qianfeng Xia, and Huangxian Ju
- Subjects
Detection limit ,Chemistry ,RNA ,Nucleic Acid Hybridization ,Biosensing Techniques ,Electrochemical Techniques ,Dengue virus ,Dengue Virus ,Cleavage (embryo) ,medicine.disease_cause ,medicine.disease ,Biochemistry ,Virology ,Rapid detection ,Amperometry ,Catalysis ,Analytical Chemistry ,Dengue fever ,Limit of Detection ,medicine ,Environmental Chemistry ,CRISPR ,Clustered Regularly Interspaced Short Palindromic Repeats ,Spectroscopy - Abstract
Dengue fever caused by Dengue virus (DENV) infection has been widely popular, especially in tropical and subtropical areas. Rapid and sensitive diagnosis is the first priority for treatment of DENV infection. This work designed a signal amplification strategy for sensitive electrochemical detection of DENV by using a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system for catalytic hairpin assembly on electrode surface. The presence of target RNA could activate the cleavage activity of the CRISPR/Cas13a system to release the blocker silenced swing arms, which then hybridized with hairpin 1 (H1) immobilized on electrode surface to expose the pre-locked toehold domain of H1 for the hybridization of ferrocene-labeled hairpin 2 (H2-Fc). Eventually, a large number of H2-Fc were captured to the electrode to produce amperometric signal for achieving signal amplification. This method showed a linear detection range from 5 fM to 50 nM with a detection limit of 0.78 fM. The proposed assay was successfully used to detect DENV type 1 in total RNA sample extracted, indicating great potential for application in early clinical diagnostic.
- Published
- 2021
47. Whole Blood Transcriptome Analysis Reveals the Correlation between Specific Immune Cells and Septicemic Melioidosis
- Author
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Qianfeng Xia, Kongning Li, Ke Xu, Jun Liu, Hua Pei, Dahua Xu, ShenTian, Xuexia Li, Li Yin, and Yunfan Quan
- Subjects
CD4-Positive T-Lymphocytes ,Medicine (General) ,Melioidosis ,Article Subject ,Clinical Biochemistry ,Bacteremia ,CD8-Positive T-Lymphocytes ,Major histocompatibility complex ,Transcriptome ,Immune system ,R5-920 ,Genetics ,medicine ,Humans ,Cytokine binding ,Molecular Biology ,biology ,Burkholderia pseudomallei ,Gene Expression Profiling ,Macrophages ,Biochemistry (medical) ,General Medicine ,Blood Proteins ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,biology.organism_classification ,Blood Physiological Phenomena ,Blood ,Gene Ontology ,Case-Control Studies ,Immunology ,biology.protein ,bacteria ,Cell adhesion molecule binding ,CD8 ,Research Article - Abstract
Melioidosis is a serious infectious disease caused by the environmental Gram-negative bacillus Burkholderia pseudomallei. It has been shown that the host immune system, mainly comprising various types of immune cells, fights against the disease. The present study was to specify correlation between septicemic melioidosis and the levels of multiple immune cells. First, the genes with differential expression patterns between patients with septicemic melioidosis (B. pseudomallei) and health donors (control/healthy) were identified. These genes being related to cytokine binding, cell adhesion molecule binding, and MHC relevant proteins may influence immune response. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed 23 enriched immune response pathways. We further leveraged the microarray data to investigate the relationship between immune response and septicemic melioidosis, using the CIBERSORT analysis. Comparison of the percentages of 22 immune cell types in B. pseudomallei vs. control/healthy revealed that those of CD4 memory resting cells, CD8+ T cells, B memory cells, and CD4 memory activated cells were low, whereas those of M0 macrophages, neutrophils, and gamma delta T cells were high. The multivariate logistic regression analysis further revealed that CD8+ T cells, M0 macrophages, neutrophils, and naive CD4+ cells were strongly associated with the onset of septicemic melioidosis, and M2 macrophages and neutrophils were associated with the survival in septicemic melioidosis. Taken together, these data point to a complex role of immune cells on the development and progression of melioidosis.
- Published
- 2021
48. Paper-based detection of Epstein-Barr virus using asymmetric polymerase chain reaction and gold silicon particles
- Author
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Haoze Chen, Shoujia Lin, Yongxia Wang, Shuqi Fu, Yueting Ma, Qianfeng Xia, and Yingzi Lin
- Subjects
Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Limit of Detection ,Environmental Chemistry ,Humans ,Metal Nanoparticles ,Biosensing Techniques ,Gold ,Silicon Dioxide ,Biochemistry ,Polymerase Chain Reaction ,Spectroscopy ,Analytical Chemistry - Abstract
A new paper-based lateral flow nucleic acid (LFNA) test platform was established in this study using asymmetric polymerase chain ceaction (A-PCR) for signal amplification. This new method allowed a visual detection of Epstein-Barr virus (EBV) nucleic acids with high specificity and low cost. In addition, as part of our strategy we employed a sandwich system of capture probe (CP)/gold nanoparticles (AuNPS) and silicon dioxide (SiO2) (AuNPS@SiO2) nanospheres/target DNA/avidin complexes as the sensing platform. Biotin-labeled target DNA was obtained by A-PCR and later introduced in the LF device. The CP/target DNA/AuNPS@SiO2 complexes were captured on the test zone by the specific reaction between biotin and avidin, and the remaining CP/AuNPS@SiO2 particles were captured on the quality control zone by the hybridization between CP and a quality control probe. Au@SiO2 accumulation in the test and quality control zones of the device enabled a visual detection of the specific target sequences. The method detection limit was 50 nM of the target DNA, which was lower than that of the LFNA biosensor (LFNAB) without PCR amplification and Au@SiO2 particles. In conclusion, the novel paper-based platform described here is a low cost, efficient and fast visual detection method that offers high sensitivity and other benefits compared to alternative methods in use.
- Published
- 2021
49. Proximity sequence enhanced CRISPR-Cas12a connected through hybridization chain reaction for sensitive biosensing of dengue virus
- Author
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Min Zhong, Jinbo Liu, Jie Wu, Jinqian Li, Nini Luo, Chuanlong Zhu, Rui Liu, Qianfeng Xia, and Huangxian Ju
- Subjects
Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
50. First Report of
- Author
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Le, Li, Vítor Manuel Pinto, Várzea, Qianfeng, Xia, Wei, Xiang, Tianle, Tang, Mengfeng, Zhu, Chunping, He, Ana Paula, Pereira, Maria do Céu Machado, Lavado da Silva, Weihuai, Wu, and Kexian, Yi
- Subjects
Basidiomycota ,Coffea ,Coffee ,Plant Diseases - Published
- 2021
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