277 results on '"Qiannan Wang"'
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2. Author Correction: Comprehensive transcriptional and functional analyses of melatonin synthesis genes in cassava reveal their novel role in hypersensitive-like cell death
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Yunxie Wei, Wei Hu, Qiannan Wang, Wei Liu, Chunjie Wu, Hongqiu Zeng, Yu Yan, Xiaolin Li, Chaozu He, and Haitao Shi
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Medicine ,Science - Published
- 2024
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3. Predictive value of 18F-FDG PET/CT radiomics for EGFR mutation status in non-small cell lung cancer: a systematic review and meta-analysis
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Ning Ma, Weihua Yang, Qiannan Wang, Caozhe Cui, Yiyi Hu, and Zhifang Wu
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non-small cell lung cancer ,EGFR mutation ,18F-FDG PET/CT ,meta-analysis ,radiomics ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveThis study aimed to evaluate the value of 18F-FDG PET/CT radiomics in predicting EGFR gene mutations in non-small cell lung cancer by meta-analysis.MethodsThe PubMed, Embase, Cochrane Library, Web of Science, and CNKI databases were searched from the earliest available date to June 30, 2023. The meta-analysis was performed using the Stata 15.0 software. The methodological quality and risk of bias of included studies were assessed using the Quality Assessment of Diagnostic Accuracy Studies 2 and Radiomics Quality Score criteria. The possible causes of heterogeneity were analyzed by meta-regression.ResultsA total of 17 studies involving 3763 non-small cell lung cancer patients were finally included. We analyzed 17 training cohorts and 10 validation cohorts independently. Within the training cohort, the application of 18F-FDG PET/CT radiomics in predicting EGFR mutations in NSCLC demonstrated a sensitivity of 0.76 (95% CI: 0.70-0.81) and a specificity of 0.78 (95% CI: 0.74-0.82), accompanied by a positive likelihood ratio of 3.5 (95% CI:3.0-4.2), a negative likelihood ratio of 0.31 (95% CI: 0.24-0.39), a diagnostic odds ratio of 11.0 (95% CI: 8.0-16.0), and an area under the curve (AUC) of 0.84 (95% CI: 0.80-0.87). In the validation cohort, the values included a sensitivity of 0.76 (95% CI: 0.67-0.83), a specificity of 0.75 (95% CI: 0.68-0.80), a positive likelihood ratio of 3.0 (95% CI:2.4-3.8), a negative likelihood ratio of 0.32 (95% CI: 0.24-0.44), a diagnostic odds ratio of 9 (95% CI: 6-15), and an AUC of 0.82 (95% CI: 0.78-0.85). The average Radiomics Quality Score (RQS) across studies was 10.47 ± 4.72. Meta-regression analysis identifies the application of deep learning and regions as sources of heterogeneity.Conclusion18F-FDG PET/CT radiomics may be useful in predicting mutation status of the EGFR gene in non-small cell lung cancer.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO, identifier CRD42022385364.
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- 2024
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4. Influence of Polypropylene Fiber on Concrete Permeability under Freeze-Thaw Conditions and Mechanical Loading
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Wei Zeng, Weiqi Wang, Qiannan Wang, Mengya Li, Lining Zhang, and Yunyun Tong
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polypropylene fiber ,permeability ,freeze-thaw damage ,tensile load ,compressive load ,concrete structure ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Polypropylene fiber reinforcement is an effective method to enhance the durability of concrete structures. With the increasing public interest in the widespread use of polypropylene fiber reinforced concrete (PFRC), the necessity of evaluating the mechanism of polypropylene fiber (PF) on the permeability of concrete has become prominent. This paper describes the influence of PF on the concrete permeability exposed to freeze-thaw cycles under compressive and tensile stress. The permeability of PFRC under compressive and tensile loads is accurately measured by a specialized permeability setup. The permeability of PFRC under compressive and tensile loads, the volume change of PFRC under compressive load, and the relationship between compressive stress levels at minimum permeability and minimum volume points of PFRC are discussed. The results indicate that the addition of PF adversely affects the permeability of concrete without freeze-thaw damage and cracks. However, it decreases the permeability of concrete specimens exposed to freeze-thaw cycles and cracking. Under compressive load, the permeability of PFRC initially decreases slowly and follows by a significant increase as the compressive stress level increases. This phenomenon correlates with the volume change of the specimen. The compressive stress level of the minimum permeability point and compressive stress level of the minimum volume point of PFRC exhibit a linear correlation, with a fitted proportional function parameter γ ≈ 0.98872. Under tensile load, the permeability of PFRC increases gradually with radial deformation and follows by a significant increase. The strain-permeability curves of PFRC under loading are studied and consist of two stages. In stage I, the permeability of PFRC gradually decreases with the increase of strain under compressive load, while the permeability increases with the increase of strain under tensile load. In stage II, under compressive load, the permeability of PFRC increases with the increase of freeze-thaw cycles, whereas under tensile load, the permeability gradually decreases with the increase of freeze-thaw cycles. The reduction of PF on the permeability of PFRC under tensile load is greater than that under compressive load. In future research, the relationship between strain and permeability of PFRC can be integrated with its constitutive relationship between stress and strain to provide a reference for the application of PF in the waterproofing of concrete structures.
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- 2024
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5. The Epidemiological Particularities of Malignant Hemopathies in French Guiana: 2005–2014
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Mathieu Nacher, Qiannan Wang, Beatrice Cenciu, Alolia Aboikoni, Florin Santa, Fabrice Quet, Fanja Vergeade, Antoine Adenis, Nathalie Deschamps, and Kinan Drak Alsibai
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malignant hemopathies ,multiple myeloma ,adult t-cell lymphoma ,HTLV-1 ,incidence ,mortality ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
French Guiana is a French Overseas territory with singular features: it has a high prevalence of HIV and HTLV-1, its population is ethnically mixed, with widespread poverty, and up to 20% of the population lives in geographic isolation. In this context, we used registry data to estimate incidence and mortality due to hematological malignancies and to compare them with France and tropical Latin America. ICD codes C90 and C88 were compiled between 2005 and 2014. The direct standardization of age structure was performed using the world population. Survival analysis was performed, and Kaplan–Meier curves were drawn. The overall standardized incidence rate was 32.9 per 100,000 male years and 24.5 per 100,000 female years. Between 2005 and 2009, the standardized incidence rate was 29.6 per 100,000 among men and 23.6 per 100,000 among women, and between 2010 and 2014, it was 35.6 per 100,000 among men and 25.2 per 100,000 among women. Multiple myeloma/plasmocytoma and mature t/NK cell lymphomas, notably adult t-cell lymphoma/leukemia due to HTLV-1 infection, were the two most common hematologic malignancies and causes of death. Non-Hodgkin’s lymphoma incidence estimates were greater than global estimates. After adjusting for age, sex, and type of malignancy, people born in a foreign country independently had a poorer case-fatality rate, presumably reflecting difficulties in accessing care. The epidemiology of hematological malignancies in French Guiana has features that distinguish it from mainland France or from Latin America. The incidence of multiple myeloma and adult t-cell lymphoma/leukemia was significantly greater in French Guiana than in France or other Latin American countries.
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- 2024
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6. Incidence of Pediatric Cancers in French Guiana: How Does It Compare to Global Estimates?
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Mathieu Nacher, Qiannan Wang, Lindsay Osei, Benjamin Faivre, Narcisse Elenga, Antoine Adenis, Nathalie Deschamps, and Kinan Drak Alsibai
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pediatric cancers ,leukemia ,central nervous system tumors ,sarcoma ,lymphoma ,French Guiana ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
French Guiana is a French territory in South America. The exposome of persons living there is quite different from that in mainland France and the ethnic make-up of the population is also quite different. Poverty is also widespread with difficulties in accessing care magnified by the low medical-professional density. In this singular context, we aimed to measure the incidence of pediatric cancers and to compare it with other continents. We used French Guiana’s certified cancer registry to study this between 2003 and 2017. Incidences were standardized using the world population with three strata: 0–4 years, 5–9 years, and 10–14 years. There were 164 solid tumors or hematologic malignancies diagnosed in children under the age of 15 (92 in boys and 72 in girls). Over the study period, the standardized incidence rate was 14.1 per 100,000 among children aged under 15 years. There was no significant trend during the study period. The three most common causes of cancer were leukemias—mostly lymphoblastic—CNS tumors, and sarcoma. The standardized incidence of pediatric cancers in French Guiana was similar to those in Western Europe and North America. As others have discovered, we found that males tended to be more likely to develop cancer, notably leukemia, CNS tumors, sarcoma, and retinoblastoma. As elsewhere, the predominant cancer types changed with age. Our initial assumption was that given the singular context of French Guiana, there may have been differences in pediatric cancer incidences. Here we showed that overall, contrary to our assumption and to trends in tropical countries, the incidence of pediatric cancers was in a range between Western Europe and North America with some apparent but non-significant differences in the main types of cancers observed in global statistics. Quality cancer registry data in this tropical region confirm the suspicion that lower incidences in tropical low- and middle-income countries are likely to result from incomplete diagnosis and data collection.
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- 2024
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7. Effect of Macro Fibers on the Permeability and Crack Surface Topography of Layered Fiber Reinforced Concrete
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Wei Zeng, Weiqi Wang, Qiannan Wang, Mengya Li, Lining Zhang, and Yunyun Tong
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permeability ,crack surface topography ,layered fiber-reinforced concrete ,layering ratio ,uniaxial tensile load ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
This paper describes the effects of macro fibers on permeability and crack surface topography of layered fiber-reinforced concrete (FRC) specimens with different layering ratios under uniaxial tensile load. The crack permeability of layered FRC specimens is investigated by a self-designed permeability setup. The topographical analysis of crack surfaces is investigated by a custom-designed laser scanning setup. The results show that when the fiber volume content and layering ratio of the FRC layer are constant, the tensile toughness of layered FRC specimens depends on the proportion of steel fiber in macro fibers, and with an increase in the proportion of steel fiber, the tensile toughness of layered FRC specimens increases. For the layered FRC specimens, the crack permeability is much lower than that of the normal concrete (NC) specimen. A significant positive synergistic effect on crack impermeability can be achieved by the combination of steel fiber and polypropylene fiber in the SF80PP2.3 specimen. The crack surface roughness parameter (Rn) values of the NC layer in layered FRC specimens are all higher than those of the NC specimen, and the crack surface Rn of the FRC layer in layered FRC specimens is higher than that of the unlayered FRC specimens. This can effectively increase the head loss of cracks and reduce the crack permeability of layered FRC specimens.
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- 2024
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8. Hybrid Fiber Influence on the Crack Permeability of Cracked Concrete Exposed to Freeze–Thaw Cycles
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Wei Zeng, Weiqi Wang, Qiannan Wang, Mengya Li, Lining Zhang, and Yunyun Tong
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crack permeability ,hybrid fiber ,crack surface ,positive synergistic effect ,splitting tensile load ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
This paper describes hybrid fiber’s influence on the crack permeability of cracked concrete exposed to freeze–thaw cycles. A permeability setup and a laser-scanning setup have been designed to measure the crack permeability and the fractured surface roughness of cracked hybrid fiber-reinforced concrete, containing polypropylene fiber and steel fiber, under a splitting tensile load. The results show that, when the effective crack width of the specimens is less than 25 μm, the rough crack surface significantly reduces the concrete’s crack permeability. As the crack width increases, the effect of the concrete crack surface on crack permeability gradually decreases, and the crack permeability of the concrete is closer to the Poiseuille flow model. The permeability parameter α derived from the Poiseuille flow model is effective for assessing the crack permeability of concrete. Compared to the modified factor ξ of crack permeability, the permeability parameter α can effectively evaluate and quantify the development trend of crack permeability within a certain range of crack widths. The permeability parameter α of SF20PP2.3, subjected to the same freeze–thaw cycles, decreases by 16.3–94.8% compared to PP4.6 and SF40, and SF20PP2.3 demonstrates a positive synergistic effect on the crack impermeability of cracked concrete. The crack impermeability of SF40PP2.3, subjected to the same freeze–thaw cycles, lies between that of PP6.9 and SF60. The roughness of crack surface (X) and the crack permeability (Y) are highly correlated and follow an exponential curve (Y = 1.0415 × 107·e−6.025·X) in concrete. This demonstrates that hybrid fibers enhance crack impermeability by increasing the crack surface roughness. Furthermore, the combination of polypropylene fiber and steel fiber effectively promotes the formation of micro-cracks and facilitates the propagation of multiple cracks in the concrete matrix. This combination increases the head loss of water flow through the concrete and decreases the crack permeability.
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- 2024
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9. Promotion methods, social learning and environmentally friendly agricultural technology diffusion: A dynamic perspective
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Yang Gao, Qiannan Wang, Chen Chen, Liqun Wang, Ziheng Niu, Xue Yao, Haoran Yang, and Jinlong Kang
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Environmentally friendly technology ,New extension pattern of agricultural technology ,Social learning ,Fertigation technology ,Duration analysis ,Ecology ,QH540-549.5 - Abstract
Encouraging farmers to adopt environmentally friendly technology through the rational use of social learning and agricultural technology extension is an effective way to overcome the bottleneck caused by the slow diffusion of environmentally friendly technology. Based on expanding the existing objects of research on farmers’ technology adoption behavior, this paper examines the influence of social learning and agricultural technology extension on farmers’ environmentally friendly technology adoption behavior from a dynamic perspective. In doing so, it enriches theoretical and empirical research on farmers’ technology adoption behavior. Specifically, this paper takes fertigation technology as an example, constructs a dynamic analysis framework that is independent of the case study, and finds that social learning and agricultural technology extension, as the main channels for farmers to obtain technical information, can shorten the duration from awareness to the adoption of fertigation technology. Then, based on survey data, this paper uses the discrete-time cloglog model to conduct an empirical test. The empirical analysis supports the theoretical analysis results, and there is a complementary effect between social learning and traditional and new agricultural technology extension. Heterogeneity analysis shows that social learning and new agricultural technology extension have a greater marginal improvement effect on farmers’ fertigation technology adoption behavior in the middle-aged to young group, middle and high education degree group and above median land scale group. This paper provides not only new empirical evidence to explain farmers' technology adoption behavior under the background of the internet revolution but also a decision-making reference for how to accelerate the construction of multivariate complementary, collaborative and efficient agricultural socialized service systems.
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- 2023
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10. Ethanolamines corrosion inhibition effect on steel rebar in simulated realkalized concrete environments
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Yunyun Tong, Jinjian Ye, Qiannan Wang, Hui Zhang, Hui Yu, and Abdel-Okash Seibou
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steel rebars ,realkalization ,ethanolamines corrosion inhibitors ,electrochemical measurements ,Architecture ,NA1-9428 ,Building construction ,TH1-9745 - Abstract
This paper reports the corrosion inhibition effect of the realkalization and corrosion inhibitors combination treatment on steel rebars. First, the corrosion was studied in simulated carbonated concrete and simulated realkalized concrete environments and then compared with the corrosion, while realkalization-corrosion inhibitor treatment was applied. The corrosion inhibitors studied are monoethanolamine (MEA), dimethylethanolamine (DEMA) and triethylenetetramine (TETA). Through the electrochemical measurement; the potential of steel rebars in realkalized environment containing MEA and DMEA shifted in positive direction by 84 mV and 66 mV; the current destiny of each group decreased by 3.76 and 2.33 μA/cm2, respectively. Unlike the addition of TETA shifted the potential in negative direction by 281 mV and increased the current density by 27.72 μA/cm2. Comparing steel rebars surfaces, the realkalization-corrosion inhibitors groups showed a better inhibition effect than the two control groups (simulated carbonated concrete and simulated realkalized environments). Among the corrosion inhibitors used, MEA has the best corrosion inhibition effect in the electrochemical realkalized concrete environment, it can form a protective film on the steel rebars through physical and chemical adsorption, which can effectively inhibit the corrosion of steel rebars. The result confirms that, by the combination of MEA and electrochemical realkalization, the corrosion inhibition in carbonated reinforced concrete can improve significantly.
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- 2023
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11. Roles of CgEde1 and CgMca in Development and Virulence of Colletotrichum gloeosporioides
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Dan Wang, Bang An, Hongli Luo, Chaozu He, and Qiannan Wang
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Colletotrichum gloeosporioides ,Ede1 ,metacaspase ,conidiation ,polar establishment ,pathogenicity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Anthracnose, induced by Colletotrichum gloeosporioides, poses a substantial economic threat to rubber tree yields and various other tropical crops. Ede1, an endocytic scaffolding protein, plays a crucial role in endocytic site initiation and maturation in yeast. Metacaspases, sharing structural similarities with caspase family proteases, are essential for maintaining cell fitness. To enhance our understanding of the growth and virulence of C. gloeosporioides, we identified a homologue of Ede1 (CgEde1) in C. gloeosporioides. The knockout of CgEde1 led to impairments in vegetative growth, conidiation, and pathogenicity. Furthermore, we characterized a weakly interacted partner of CgEde1 and CgMca (orthologue of metacaspase). Notably, both the single mutant ΔCgMca and the double mutant ΔCgEde1/ΔCgMca exhibited severe defects in conidiation and germination. Polarity establishment and pathogenicity were also disrupted in these mutants. Moreover, a significantly insoluble protein accumulation was observed in ΔCgMca and ΔCgEde1/ΔCgMca strains. These findings elucidate the mechanism by which CgEde1 and CgMca regulates the growth and pathogenicity of C. gloeosporioides. Their regulation involves influencing conidiation, polarity establishment, and maintaining cell fitness, providing valuable insights into the intricate interplay between CgEde1 and CgMca in C. gloeosporioides.
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- 2024
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12. CgCFEM1 Is Required for the Full Virulence of Colletotrichum gloeosporioides
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Liping Feng, Meixia Dong, Zhirui Huang, Qian Wang, Bang An, Chaozu He, Qiannan Wang, and Hongli Luo
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Colletotrichum gloeosporioides ,CgCFEM1 ,appressorium morphogenesis ,plant immunity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Colletotrichum gloeosporioides is widely distributed and causes anthracnose on many crops, resulting in serious economic losses. Common fungal extracellular membrane (CFEM) domain proteins have been implicated in virulence and their interaction with the host plant, but their roles in C. gloeosporioides are still unknown. In this study, a CFEM-containing protein of C. gloeosporioides was identified and named as CgCFEM1. The expression levels of CgCFEM1 were found to be markedly higher in appressoria, and this elevated expression was particularly pronounced during the initial stages of infection in the rubber tree. Absence of CgCFEM1 resulted in impaired pathogenicity, accompanied by notable perturbations in spore morphogenesis, conidiation, appressorium development and primary invasion. During the process of appressorium development, the absence of CgCFEM1 enhanced the mitotic activity in both conidia and germ tubes, as well as compromised conidia autophagy. Rapamycin was found to basically restore the appressorium formation, and the activity of target of rapamycin (TOR) kinase was significantly induced in the CgCFEM1 knockout mutant (∆CgCFEM1). Furthermore, CgCFEM1 was proved to suppress chitin-triggered reactive oxygen species (ROS) accumulation and change the expression patterns of defense-related genes. Collectively, we identified a fungal effector CgCFEM1 that contributed to pathogenicity by regulating TOR-mediated conidia and appressorium morphogenesis of C. gloeosporioides and inhibiting the defense responses of the rubber tree.
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- 2024
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13. Structural basis of human TREX1 DNA degradation and autoimmune disease
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Wen Zhou, Desmond Richmond-Buccola, Qiannan Wang, and Philip J. Kranzusch
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Science - Abstract
TREX1 is a cytosolic DNA nuclease and the genetic mutations of it are linked to autoimmune diseases. Here the authors determine the first structure of the human TREX1–DNA complex, and provide a new foundation to explain how patient TREX1 mutations cause autoimmune disease.
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- 2022
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14. Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.
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Qiannan Wang, Yanan Xu, Shuangshuang Zhao, Yuxiang Jiang, Ran Yi, Yan Guo, and Shanjin Huang
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Biology (General) ,QH301-705.5 - Abstract
As the stimulus-responsive mediator of actin dynamics, actin-depolymerizing factor (ADF)/cofilin is subject to tight regulation. It is well known that kinase-mediated phosphorylation inactivates ADF/cofilin. Here, however, we found that the activity of Arabidopsis ADF7 is enhanced by CDPK16-mediated phosphorylation. We found that CDPK16 interacts with ADF7 both in vitro and in vivo, and it enhances ADF7-mediated actin depolymerization and severing in vitro in a calcium-dependent manner. Accordingly, the rate of actin turnover is reduced in cdpk16 pollen and the amount of actin filaments increases significantly at the tip of cdpk16 pollen tubes. CDPK16 phosphorylates ADF7 at Serine128 both in vitro and in vivo, and the phospho-mimetic mutant ADF7S128D has enhanced actin-depolymerizing activity compared to ADF7. Strikingly, we found that failure in the phosphorylation of ADF7 at Ser128 impairs its function in promoting actin turnover in vivo, which suggests that this phospho-regulation mechanism is biologically significant. Thus, we reveal that CDPK16-mediated phosphorylation up-regulates ADF7 to promote actin turnover in pollen.
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- 2023
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15. The Singular Epidemiology of Plasmacytoma and Multiple Myeloma in French Guiana
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Laure Manuella Imounga, Kinan Drak Alsibai, Juliette Plenet, Qiannan Wang, Beatrice Virjophe-Cenciu, Pierre Couppie, Nadia Sabbah, Antoine Adenis, and Mathieu Nacher
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multiple myeloma ,plasmacytoma ,French Guiana ,incidence ,mortality ,Latin America ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: The objective was to review a decade of plasmacytoma (PC) and multiple myeloma (MM) data from French Guiana, and to study its spatial and temporal trends. Methods: This was a retrospective study of MM and PC between January 2005 and December 2014 using cancer registry data, including age-standardized incidence and mortality rates. Results: There were 110 cases of PC and MM (62 women and 48 men), representing the eighth most frequent malignancy in French Guiana. PC and MM were much more common in females. In men, 79% of cases occurred at ≥55 years, and in women, 90% of cases occurred at ≥50 years. The median age at diagnosis was 60 years for men and 66 years for women, while it was 72 years for men and 75 years for women in mainland France. The incidence rate standardized to the world population was 5.9 patients of PC and MM per 100,000 men/year and 7.8 per 100,000 women/year. Conclusions: In our territory, the incidence of PC and MM was higher and patients were diagnosed at a substantially younger age than in mainland France. Women had a greater incidence than men, and there was an increasing temporal trend of incidence among women. African ancestry and the frequency of obesity, notably among women, could have contributed to this observation.
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- 2023
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16. AGE/RAGE axis regulates reversible transition to quiescent states of ALK-rearranged NSCLC and pancreatic cancer cells in monolayer cultures
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Tetsuya Kadonosono, Kotaro Miyamoto, Shiori Sakai, Yoshiyuki Matsuo, Shojiro Kitajima, Qiannan Wang, Minori Endo, Mizuho Niibori, Takahiro Kuchimaru, Tomoyoshi Soga, Kiichi Hirota, and Shinae Kizaka-Kondoh
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Medicine ,Science - Abstract
Abstract Cancer recurrence due to tumor cell quiescence after therapy and long-term remission is associated with cancer-related death. Previous studies have used cell models that are unable to return to a proliferative state; thus, the transition between quiescent and proliferative states is not well understood. Here, we report monolayer cancer cell models wherein the human non-small cell lung carcinoma cell line H2228 and pancreatic cancer cell line AsPC-1 can be reversibly induced to a quiescent state under hypoxic and serum-starved (HSS) conditions. Transcriptome and metabolome dual-omics profiles of these cells were compared with those of the human lung adenocarcinoma cell line A549, which was unable to enter a quiescent state under HSS conditions. The quiescence-inducible cells had substantially lower intracellular pyruvate and ATP levels in the quiescent state than in the proliferative state, and their response to sudden demand for energy was dramatically reduced. Furthermore, in quiescence-inducible cells, the transition between quiescent and proliferative states of these cells was regulated by the balance between the proliferation-promoting Ras and Rap1 signaling and the suppressive AGE/RAGE signaling. These cell models elucidate the transition between quiescent and proliferative states, allowing the development of drug-screening systems for quiescent tumor cells.
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- 2022
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17. The histone deacetylase HOS2 controls pathogenicity through regulation of melanin biosynthesis and appressorium formation in Colletotrichum gloeosporioides
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Shike Liu, Qiannan Wang, Na Liu, Hongli Luo, Chaozu He, and Bang An
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Histone deacetylase HOS2 ,Colletotrichum gloeosporioides ,Pathogenicity ,Melanin ,Appressorium ,Plant culture ,SB1-1110 - Abstract
Abstract The reversible acetylation of histones is effective for controlling chromatin dynamics and plays crucial roles in eukaryotes. In the present study, we identified and characterized a histone deacetylase HOS2 ortholog, designated CgHOS2, in Colletotrichum gloeosporioides. Knocking out CgHOS2 resulted in decreased vegetative growth, impaired conidiation, and reduced stress tolerance. Moreover, the ΔCgHOS2 mutant failed to form appressoria and lost pathogenicity on intact plant leaves. Western blot analysis revealed that CgHOS2 is responsible for the deacetylation of histone H3. Via transcriptomic analysis, a series of candidate genes controlled by CgHOS2 were predicted. Of these candidate genes, the expression of melanin biosynthesis-related enzymes was significantly reduced in vegetative hyphae and especially in appressoria, which led to a decrease in melanin content and failure of appressorium formation. Taken together, these results highlight the role of CgHOS2 in pathogenicity via regulation of melanin biosynthesis in C. gloeosporioides.
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- 2022
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18. TaBAS1 encoding a typical 2-Cys peroxiredoxin enhances salt tolerance in wheat
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Guilian Xiao, Mingming Zhao, Qinghua Liu, Junzhi Zhou, Zhaohui Cheng, Qiannan Wang, Guangmin Xia, and Mengcheng Wang
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wheat ,salt ,peroxiredoxin ,ROS ,yield ,Plant culture ,SB1-1110 - Abstract
Efficient antioxidant enzymatic system contributes to salt tolerance of plants via avoiding ROS over-accumulation. Peroxiredoxins are crucial components of the reactive oxygen species (ROS) scavenging machinery in plant cells, but whether they offer salt tolerance with potential for germplasm improvement has not been well addressed in wheat. In this work, we confirmed the role of a wheat 2-Cys peroxiredoxin gene TaBAS1 that was identified through the proteomic analysis. TaBAS1 overexpression enhanced the salt tolerance of wheat at both germination and seedling stages. TaBAS1 overexpression enhanced the tolerance to oxidative stress, promoted the activities of ROS scavenging enzymes, and reduced ROS accumulation under salt stress. TaBAS1 overexpression promoted the activity of ROS production associated NADPH oxidase, and the inhibition of NADPH oxidase activity abolished the role of TaBAS1 in salt and oxidative tolerance. Moreover, the inhibition of NADPH-thioredoxin reductase C activity erased the performance of TaBAS1 in the tolerance to salt and oxidative stress. The ectopic expression of TaBAS1 in Arabidopsis exhibited the same performance, showing the conserved role of 2-Cys peroxiredoxins in salt tolerance in plants. TaBAS1 overexpression enhanced the grain yield of wheat under salt stress but not the control condition, not imposing the trade-offs between yield and tolerance. Thus, TaBAS1 could be used for molecular breeding of wheat with superior salt tolerance.
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- 2023
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19. Colletotrichum gloeosporioides Cg2LysM contributed to virulence toward rubber tree through affecting invasive structure and inhibiting chitin-triggered plant immunity
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Li Zhao, Zhiwen Liao, Liping Feng, Bang An, Chaozu He, Qiannan Wang, and Hongli Luo
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Colletotrichum gloeosporioides ,Cg2LysM ,pathogenesis ,chitin binding ,rubber tree ,plant immunity ,Microbiology ,QR1-502 - Abstract
Fungal chitin, as a typical microorganism-associated molecular pattern (PAMP), was recognized by plant LysM-containing protein to induce immunity called pattern-triggered immunity (PTI). To successfully infect host plant, fungal pathogens secreted LysM-containing effectors to inhibit chitin-induced plant immunity. Filamentous fungus Colletotrichum gloeosporioides caused rubber tree anthracnose which resulted in serious loss of natural rubber production worldwide. However, little is known about the pathogenesis mediated by LysM effector of C. gloeosporioide. In this study, we identified a two LysM-containing effector in C. gloeosporioide and named as Cg2LysM. Cg2LysM was involved not only in conidiation, appressorium formation, invasion growth and the virulence to rubber tree, but also in melanin synthesis of C. gloeosporioides. Moreover, Cg2LysM showed chitin-binding activity and suppression of chitin-triggered immunity of rubber tree such as ROS production and the expression of defense relative genes HbPR1, HbPR5, HbNPR1 and HbPAD4. This work suggested that Cg2LysM effector facilitate infection of C. gloeosporioides to rubber tree through affecting invasive structure and inhibiting chitin-triggered plant immunity.
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- 2023
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20. Systematic analysis of the role and significance of target genes of active ingredients of traditional Chinese medicine injections in the progression and immune microenvironment of hepatocellular carcinoma
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Chao Wang, Lili Yang, Shaoheng Xu, Hui Guo, Hewen Guan, Qiannan Wang, Xueyan Jiang, Mingyang Fei, and Jinbao Zhang
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hepatocellular carcinoma ,traditional Chinese medicine ,immune microenvironment ,injection ,adjuvant therapy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Traditional Chinese medicine in China is an important adjuvant therapy for the treatment of hepatocellular carcinoma (HCC) and traditional Chinese medicines injections have a wide range of clinical applications. The purpose of this study was to identify the active ingredients and related genes of traditional Chinese medicine injections that can treat hepatocellular carcinoma.Methods: Effective small molecule components were extracted from 14 types of traditional Chinese medicines from 8 injections and the main gene targets were identified. The 968 patients with HCC were classified based on the target gene set, and the characteristics of patients with different subtypes were analyzed. Patients with two subtypes of HCC were compared with normal tissues and cirrhosis to identify important gene targets related to traditional Chinese medicines in HCC progression.Results: In this study, 138 important genes associated with traditional Chinese medicines were identified and two HCC subtypes were identified. By analyzing the differences between the two subtypes, 25 related genes were associated with HCC subtypes. Through clinical and pharmacological analysis, this study identified quercetin as an important traditional Chinese medicines small molecule and secreted phosphoprotein 1 (SPP1) as an important oncogene in HCC.Conclusion: Traditional Chinese medicines injection is an important adjuvant treatment modality for HCC. SPP1 is an important oncogene in HCC.
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- 2023
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21. Efficient Solution-Processed Blue and Yellow Phosphorescent Organic Light-emitting Diodes Using Binary Blend Hosts
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Yuxin GUAN, Wenjing LIN, Qiannan WANG, Pengchao ZHOU, Bin WEI, and Yingjie LIAO
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organic light-emitting diodes ,solution-processed ,binary blend ,Mining engineering. Metallurgy ,TN1-997 - Abstract
The appropriate hosts of emitting layers (EMLs) play an important role in determining the overall performance of solution-processed phosphorescent organic light emitting diodes (PhOLEDs). We have investigated the effect of three species of host molecules, 1,3-bis(carbazol-9-yl)benzene (mCP), 10-(4-(5,5dimethylbenzofuro[3,2-c]acridin-13(5H)-yl)phenyl)-10-phenylanthracen-9(10H)-one (DpAn-5BzAc) and poly(9-vinylcarbazole) (PVK), on the performance of solution-processed blue and yellow PhOLEDs. We have found that compared to the widely used single-host EMLs, the devices using the binary blend of mCP: DpAn5BzAc as hosts, can achieve more efficient optoelectrical characteristics. The maximum current efficiencies of 11.84 and 16.61 have been realized for blue and yellow OLEDs, respectively. The superior electroluminescence performance for binary blend host-based PhOLEDs was attributed to the enhanced charge carrier balance and multi-component miscibility, which has a dramatic influence on the morphology of the emissive layer. These results demonstrate the great potential of the multi-hosts in solution-processed organic optoelectronic devices. The development of complementary colour OLEDs with blue and yellow can provide a simple approach to fabricate solution-processed white PhOLEDs.
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- 2022
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22. Shear band mediated ω phase transformation in Nb single crystals deformed at 77 K
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Xiyao Li, Qingkun Zhao, Qiannan Wang, Yanzhong Tian, Haofei Zhou, and Jiangwei Wang
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body-centered cubic ,niobium ,ω phase ,shear band ,bcc-to-ω phase transformation ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Phase transformation is rare in pure body-centered cubic (BCC) metals due to the associated high energy barrier. Here, omega (ω) phase transformation in single-crystal niobium (Nb) under compression at 77 K was studied by atomistic characterization and density functional theory (DFT) calculation. The ω phases were found to nucleate preferentially at the boundaries of 112 shear bands, via a sequence of BCC-to-ω structural transformation induced by shear stress. Theoretically, calculations reveal that the formation of a shear transition region can reduce the energy barrier of BCC-to-ω phase transformation. These findings provide insights into the phase transformation involved in the cryogenic deformation of bulk BCC metals.
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- 2021
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23. CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis
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Na Liu, Qiannan Wang, Chaozu He, and Bang An
- Subjects
Colletotrichum gloeosporioides ,major facilitator superfamily ,oxidative resistance ,pathogenicity ,Biology (General) ,QH301-705.5 - Abstract
Colletotrichum gloeosporioides is the main causal agent of anthracnose in various plant species. Determining the molecular mechanisms underlying the pathogenicity and fungicide resistance of C. gloeosporioides could help build new strategies for disease control. The major facilitator superfamily (MFS) has multiple roles in the transport of a diverse range of substrates. In the present study, an MFS protein CgMFS1 was characterized in C. gloeosporioides. This protein contains seven transmembrane domains, and its predicted 3D structure is highly similar to the reported hexose transporters. To investigate the biological functions of CgMFS1, the gene knock-out mutant ΔCgMFS1 was constructed. A colony growth assay showed that the mutant was remarkably decreased in vegetative growth in minimal medium supplemented with monosaccharides and oligosaccharides as the sole carbon sources, whereas it showed a similar growth rate and colony morphology as wild types when using soluble starch as the carbon source. A stress assay revealed that CgMFS1 is involved in oxidative stress but not in the fungicide resistance of C. gloeosporioides. Furthermore, its pathogenicity was significantly impaired in the mutant, although its appressorium formation was not affected. Our results demonstrate that CgMFS1 is required for sugar transport, resistance to oxidative stress, and the pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.
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- 2021
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24. The role and mechanism of NLRP3 inflammasome-mediated astrocyte activation in dehydrocorydaline against CUMS-induced depression
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Yu Fang, Hong Guo, Qiannan Wang, Congcong Liu, Shuyi Ge, and Bohua Yan
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depression ,CUMS ,NLRP3 inflammasome ,astrocyte activation ,dehydrocorydaline ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Depression is a common and potentially life-threatening mental illness, and currently, there is a lack of effective treatment. It has been reported that dehydrocorydaline (DHC) can inhibit monoamine transporter uptake in depressed CUMS mice, but more possible mechanisms of action remain to be further studied.Methods: C57BL/6 mice were exposed to chronic unpredictable mild stress (CUMS) for five consecutive weeks. The mice were administrated with dehydrocorydaline or fluoxetine (FLU) for four consecutive weeks. Behavioral tests including sucrose preference test (SPT), tail suspension test (TST), and forced swimming test (FST) were applied. In parallel, hematoxylin and eosin (H&E) staining and Nissl staining were used to explore the effect of DHC on pathological changes in the hippocampus. The concentrations of depression-related factors (5-HT and DA) and inflammatory factors (TNF-α, IL-6, and IL-1β) in the hippocampus and serum were assessed by ELISA assay. NLRP3 inflammasome pathway-related proteins (NLRP3, IL-18, IL-1 IL-1α, and caspase-1) were detected by western blot. The activation of microglia and astrocytes was subjected to immunofluorescent staining. Additionally, microglia were treated with DHC (100 mg/L) for 24 h following incubation with 100 ng/ml LPS for 12 h. ov-NC or ov-NLRP3 plasmid was transfected into microglia 6 h before LPS induction for exploring the effect of NLRP3 overexpression on DHC-inhibited microglia activation. Then, conditioned media of microglia were collected from each group, followed by intervention of astrocytes for 24 h to explore the effect of NLRP3 overexpression of microglia on astrocyte activation.Results:In vivo administration of DHC was found to ameliorate depressive-like behaviors and attenuate neuron damage of CUMS mice. DHC increased neurotransmitter concentration, reduced the proinflammatory factor levels, attenuated NLRP3 inflammasome activation, and decreased A1 astrocyte and microglia activation in the hippocampus of CUMS mice. Furthermore, in vivo results showed that activated microglia induced activation of A1 astrocytes but not A2 astrocytes.Conclusion: Taken together, we provided evidence that DHC exhibited antidepressive effects on CUMS mice possibly via NLRP3 inflammasome-mediated astrocyte activation.
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- 2022
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25. A Novel Effector, FSE1, Regulates the Pathogenicity of Fusarium oxysporum f. sp. cubense Tropical Race 4 to Banana by Targeting the MYB Transcription Factor MaEFM-Like
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Yongbao Yang, Bang An, Yunfeng Guo, Hongli Luo, Chaozu He, and Qiannan Wang
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Fusarium oxysporum ,banana wilt disease ,effector ,interaction ,hypersensitive reaction ,Biology (General) ,QH301-705.5 - Abstract
Phytopathogenic fungi secretes a range of effectors to manipulate plant defenses. Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a soil-borne pathogen that causes destructive banana wilt disease. Understanding the molecular mechanisms behind Foc TR4 effectors and their regulation of pathogenicity is helpful for developing disease control strategies. In the present study, we identified a novel effector, Fusarium special effector 1 (FSE1), in Foc TR4. We constructed FSE1 knock-out and overexpression mutants and investigated the functions of this effector. In vitro assays revealed that FSE1 was not required for vegetative growth and conidiation of Foc TR4. However, inoculation analysis of banana plantlets demonstrated that knock-out of FSE1 increased the disease index, while overexpression of FSE1 decreased it. Microscope analysis suggested that FSE1 was distributed in the cytoplasm and nuclei of plant cells. Furthermore, we identified an MYB transcription factor, MaEFM-like, as the target of FSE1, and the two proteins physically interacted in the nuclei of plant cells. In addition, Transient expression of MaEFM-like induced cell death in tobacco leaves. Our findings suggest that FSE1 is involved in the pathogenicity of Foc TR4 by targeting MaEFM-like.
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- 2023
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26. Hyperemesis gravidarum induced refeeding syndrome causes blood cell destruction: a case report and literature review
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Xiyu Pan, Ran Chu, Jinyu Meng, Qiannan Wang, Yue Zhang, Kun Song, Xingsheng Yang, and Beihua Kong
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Hyperemesis gravidarum ,Refeeding syndrome ,Hypophosphatemia ,Intravascular hemolytic anemia ,Thrombocytopenia ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Background Hyperemesis gravidarum (HG) is a common complication during pregnancy, however, HG associated simultaneous onset of blood cell destruction due to electrolyte abnormalities is rare. In this case, a woman with refeeding syndrome (RFS) secondary to electrolyte abnormalities caused by severe HG was diagnosed and managed in our hospital. Case presentation A 29-year old woman was sent to the local hospitals because of severe HG with appetite loss, weight reduction, general fatigue, and she was identified to have severe electrolyte abnormalities. However, the electrolyte abnormalities were not corrected promptly, and then she had the symptoms of stillbirth, altered mental status, visual hallucination, hemolytic anemia and thrombocytopenia. After transferred to our hospital, we continued to correct the electrolyte abnormalities and the labor induction was performed as soon as possible. The symptoms of blood cell destruction were relieved obviously, and the patient discharged four days later. The electrolyte disturbances and physio-metabolic abnormalities caused by HG helped us diagnose this case as RFS. Conclusions This case emphasizes that patients with RFS should be diagnosed appropriately and intervened promptly in order to prevent electrolyte imbalance induced blood cell destruction.
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- 2021
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27. NADPH Oxidases Play a Role in Pathogenicity via the Regulation of F-Actin Organization in Colletotrichum gloeosporioides
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Na Liu, Wenfeng Wang, Chaozu He, Hongli Luo, Bang An, and Qiannan Wang
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actin cytoskeleton ,appressorium ,Colletotrichum gloeosporioides ,NADPH oxidases ,polarized growth ,Microbiology ,QR1-502 - Abstract
Multiunit-flavoenzyme NADPH oxidases (NOXs) play multiple roles in living cells via regulating signaling pathways. In several phytopathogenic fungi, NOXs are required for the polarized growth of hyphal tips and pathogenicity to host plants, but the possible mechanisms are still elusive. In our previous study, CgNOXA, CgNOXB, and CgNOXR were identified as components of the NOX complex in Colletotrichum gloeosporioides. The growth and the inoculation assays revealed that CgNOXA/B and CgNOXR regulate vegetative growth and are required for the full pathogenicity of C. gloeosporioides to Hevea leaves. We further demonstrated that the vital roles of CgNOXB and CgNOXR in appressorium formation and the development of invasion hyphae account for their functions in pathogenicity. Moreover, CgNOXB and CgNOXR regulate the production and distribution of ROS in hyphal tips and appressoria, control the specialized remodeling of F-actin in hyphal tips and appressoria, and are involved in fungal cell wall biosynthesis. Taken together, our findings highlight the role of NOXs in fungal pathogenicity through the organization of the actin cytoskeleton.
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- 2022
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28. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
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Guangyong Yang, Jie Yang, Qiwei Zhang, Wenfeng Wang, Liping Feng, Li Zhao, Bang An, Qiannan Wang, Chaozu He, and Hongli Luo
- Subjects
Colletotrichum gloeosporioides ,CgNLP1 ,pathogenic mechanism ,Hevea brasiliensis ,HbMYB8-like ,Microbiology ,QR1-502 - Abstract
Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like protein (NLP) effector gene, CgNLP1, which contributed to conidial germination, appressorium formation, invasive growth, and virulence of Colletotrichum gloeosporioides to the rubber tree. Transient expression of CgNLP1 in the leaves of Nicotiana benthamiana induced ethylene production in plants. Ectopic expression of CgNLP1 in Arabidopsis significantly enhanced the resistance to Botrytis cinerea and Alternaria brassicicola. An R2R3 type transcription factor HbMYB8-like of rubber tree was identified as the target of CgNLP1.HbMYB8-like, localized on the nucleus, and induced cell death in N. benthamiana. CgNLP1 disrupted nuclear accumulation of HbMYB8-like and suppressed HbMYB8-like induced cell death, which is mediated by the salicylic acid (SA) signal pathway. This study suggested a new strategy whereby C. gloeosporioides exploited the CgNLP1 effector to affect invasion and suppress a host defense regulator HbMYB8-like to facilitate infection.
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- 2022
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29. A SIMPLE MODEL FOR THE CHLORIDE DIFFUSION COEFFICIENT OF STEEL FIBRE REINFORCED CONCRETE
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Mwila Chitakwa, Qiannan Wang, Peng Yuxiang, Gao Zesu, Zhang Weibin, Yang Lixia, and Gu Chunping
- Subjects
chloride ,porosity ,paste ,interfacial transition zone ,general self-consistent scheme ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
The chloride diffusion coefficient is the most important parameter when describing the chloride ingress in concrete. This paper proposes a simple model to predict the chloride diffusion coefficient of steel fibre reinforced concrete (SFRC). The chloride diffusion coefficients of a cement paste and an interfacial transition zone (ITZ) were calculated with their porosities, respectively. The ITZ porosity was obtained with a w/b ratio based on a porosity model. The chloride diffusion coefficient of the matrix was then determined according to the general self-consistent scheme, and the influence of the steel fibre was simplified with its volume fraction. The calculated chloride diffusion coefficient of the SFRC was verified with the results from the bulk diffusion tests and the accuracy was acceptable.
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- 2021
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30. Two cerato-platanin proteins FocCP1 interact with MaPR1 and contribute to virulence of Fusarium oxysporum f. sp. cubense to banana
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Qingdeng Feng, Xuesheng Gao, Bang An, Chaozu He, and Qiannan Wang
- Subjects
fusarium oxysporum f. sp. cubense ,cerato-platanin ,virulence ,interaction ,pr1 ,Plant culture ,SB1-1110 ,Plant ecology ,QK900-989 - Abstract
Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) causes destructive wilt disease to banana cultivation. However, knowledge of mechanisms in virulence regulation of Foc TR4 is still inadequate. In our previous work, two hypothetical FocCP1 proteins that belong to the cerato-platanin (CP) family were identified in the secretome of Foc TR4. In the present study, knock-out mutants of two FocCP1 were constructed and functions of two proteins were investigated. The results showed that the two FocCP1 play important roles in regulation of full virulence but are not required for vegetative growth or conidiation of the pathogen; besides, there is a functional redundancy between the two FocCP1. Moreover, we found that FocCP1 proteins interact with MaPR1 proteins through yeast two-hybrid and BiFC test. Our results suggest that FocCP1 proteins are important for the virulence of Foc TR4 and might inhibit the antifungal activity of MaPR1 by directly interacting with them.
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- 2021
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31. Investigation on the Carbonation Behavior of Alkali-Activated Pastes Served under Windy Environments
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Dong Cui, Lingshu Shen, Yidong Shen, Guantong Han, Xiaoying Xie, Qianfei Cao, Jing Wang, Hao Wei, Qiannan Wang, and Keren Zheng
- Subjects
alkali-activated slag ,carbonation ,wind environment ,cementitious materials ,durability ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Most reinforced concrete structures serve under windy environments, and the carbonation resistance under that circumstance exhibits significant difference from that under the steady (no wind) environment. In this study, a windy environment was simulated using one self-developed wind tunnel, and alkali-activated slag/fly ash paste specimens were adopted for the carbonation under variant windy environments. Meanwhile, to reveal the effect of inner humidity on the carbonation, sliced alkali-activated materials (AAM) were mass-balanced first to variant humidity, and were then carbonated under a 2.5 m/s windy environment. With the assistance of computed tomography (CT), the structure of AAM at variant carbonation ages was rendered. The experimental result showed that wind is capable of promoting the exchange of moisture between the sample inside and the outer atmosphere, leading to faster carbonation as compared to that under no wind environment. When preconditioned to lower inner humidity, the carbonation rate of AAM was faster because the larger gaseous space benefited the intrusion of both CO2 and moisture. Furthermore, when preconditioned to lower humidity, the cracking extent of AAM was severer, which also contributed to the faster carbonation. Moreover, compared with ordinary Portland cement (OPC), the carbonation front on each instant 1D gray-scale value profile was broader, which suggested that the carbonation progress of AAM under windy environments was no longer controlled solely by diffusion. In addition, the gray-scale value on instant 1D profile fluctuated drastically, which verified cracking in AAM carbonated under windy environments. The current work not only deepens the understanding of the carbonation mechanism in-site (mostly under windy environments), but also helps to develop more environment-friendly construction material, with better durability performance.
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- 2023
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32. Label free proteomics and systematic analysis of secretome reveals effector candidates regulated by SGE1 and FTF1 in the plant pathogen Fusarium oxysporum f. sp. cubense tropical race 4
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Shixue Zhao, Bang An, Yanhua Guo, Xingrong Hou, Hongli Luo, Chaozu He, and Qiannan Wang
- Subjects
F. oxysporum f. sp. cubense ,Secretome ,Label free proteomics ,Effectors ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Phytopathogens secreted effectors during host colonization to suppress or trigger plant immunity. Identification of new effectors is one of the research focuses in recent years. There is only a limited knowledge about effectors of Fusarium oxysporum f. sp. Cubense tropical race 4 (Foc TR4), the causal agent of wilt disease in Cavendish banana. Results Two transcription factors, SGE1 and FTF1, were constitutively over-expressed in Foc TR4 to partially mimic the in-planta state. Secreted proteins with high purity were prepared through a two-round extraction method. Then the secretome were analyzed via label free proteomics method. A total of 919 non-redundant proteins were detected, of which 74 proteins were predicted to be effector candidates. Among these candidates, 29 were up-regulated and 13 down-regulated in the strain over-expressing SGE1 and FTF1, 8 were up-regulated and 4 down-regulated in either SGE1 or FTF1 over expression strain. Conclusions Through label free proteomics analysis, a series of effector candidates were identified in secretome of Foc TR4. Our work put a foundation for functional research of these effectors.
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- 2020
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33. Research on Integrated Optimization Design Method of High-Efficiency Motor Propeller System for UAVs With Multi-States
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Dengyan Duan, Zhigang Wang, Qiannan Wang, and Jianbo Li
- Subjects
Electric aircraft ,motor ,propeller ,integrated design ,improved PSODE optimization ,tilt quad rotor ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
With the popularity of electric multi-rotor unmanned aerial vehicles (EMRUAV), the problem of low efficiency of the motor propeller system (MPS) becomes serious especially for EMRUAVs which have multiple working states. To solve this problem, an integrated optimization design method for high-efficiency MPS is proposed using an improved parallel particle swarm optimization and differential evolution (PSODE) hybrid algorithm. First, the brushless motor as well as the variable-speed and the adjustable-pitch propellers is modelled. Then considering the characteristics of the motor and the propeller sufficiently, an integrated optimization method for variable-speed and adjustable-pitch MPS is proposed by utilizing the improved PSODE. Specially, the self-organizing feature map theory is added to substitute the traditional “winner-take-all” method in the interactive study of the improved PSODE. Next, the optimization of a small electric-powered tilt quad rotor (TQR) which has four states: hovering, cruising, transition and maximum speed is carried out. At last, the wind-tunnel experiments are implemented to verify the feasibility and effectiveness of the proposed optimization method. The results indicate that the adjustable-pitch MPS is more suitable for EMRUAVs which have multiple working states, and that the improved PSODE has faster convergence speed and better global search ability than other optimization methods.
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- 2020
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34. Role of Abdominal Aortic Balloon Placement in Planned Conservative Management of Placenta Previa With Placenta Increta or Percreta
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Ruihui Lu, Ran Chu, Qiannan Wang, Yintao Xu, Ying Zhao, Guowei Tao, Qi Li, and Yuyan Ma
- Subjects
balloon placement in the abdominal aorta ,placenta accreta spectrum ,cesarean delivery ,planned conservative management ,adverse maternal event ,Medicine (General) ,R5-920 - Abstract
Background: We investigated the role of balloon placement in the abdominal aorta (BPAA) in planned conservative management of placenta previa with placenta increta or percreta and the effects of BPAA on perinatal adverse maternal events.Methods: This retrospective case-control study included women with placenta previa (increta or percreta), who underwent pregnancy termination at the Qilu Hospital of Shandong University between January 2016 and June 2019. Patients were categorized into the BPAA and non-BPAA groups based on the BPAA placement before delivery. The Chi-square and non-parametric rank-sum tests were used for the intergroup comparison of patient characteristics. The propensity score matching algorithm was used to minimize the intergroup differences in clinical characteristics. Logistic regression analysis was used to identify the factors associated with a high risk of adverse pregnancy outcomes. The area under the receiver operating characteristic curve [area under the curve (AUC)] was used to evaluate the classification of the selected high-risk factors.Results: The study included 260 patients, and 104 patients were identified after propensity score matching. In the post-matched cohort, intraoperative blood loss was significantly lower in the BPAA than in the non-BPAA group (median 1,000 vs. 2,250 ml, P < 0.001). Intraoperative B-Lynch suture was performed in fewer patients in the BPAA (15.4 vs. 34.6%, P = 0.024) than in the non-BPAA group. The packed red blood cell (PRBC) transfusion rate was lower in the BPAA group (median 4 vs. 8 units, P < 0.001). Overall, 46 (45.1%) patients developed adverse maternal events; however, the rate of adverse maternal events was lower in the BPAA group (19.6 vs. 80.4%, P < 0.001). No ligation of the ascending branch of the uterine artery (P = 0.034), no BPAA (P < 0.001), intraplacental vascular lacunae (P = 0.046), and cervical hypervascularity (P = 0.001) were associated with a high risk of adverse perinatal maternal events. The AUC of the high-risk factors was 0.89 in the post-matched and 0.76 in the pre-matched cohorts.Conclusion: Planned conservative management using BPAA significantly minimized the intraoperative blood loss, the need for a B-Lynch suture, and PRBC transfusion in patients with severe placenta accreta spectrum and placenta previa.
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- 2021
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35. Two Facile Aniline-Based Hypercrosslinked Polymer Adsorbents for Highly Efficient Iodine Capture and Removal
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Biying Liu, Chaochao Mao, Zian Zhou, Qiannan Wang, Xiong Zhou, Zhijie Liao, Ran Deng, Defei Liu, Jingzi Beiyuan, Daofei Lv, Jiesen Li, Liyun Huang, Xin Chen, and Wenbing Yuan
- Subjects
hypercrosslinked polymer ,aniline ,iodine capture ,iodine water ,iodine vapor ,adsorption mechanism ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Effective capture and safe disposal of radioactive iodine (129I or 131I) during nuclear power generation processes have always been a worldwide environmental concern. Low-cost and high-efficiency iodine removal materials are urgently needed. In this study, we synthesized two aniline-based hypercrosslinked polymers (AHCPs), AHCP-1 and AHCP-2, for iodine capture in both aqueous and gaseous phases. They are obtained by aniline polymerization through Friedel–Crafts alkylation and Scholl coupling reaction, respectively, with high chemical and thermal stability. Notably, AHCP-1 exhibits record-high static iodine adsorption (250 wt%) in aqueous solution. In the iodine vapor adsorption, AHCP-2 presents an excellent total iodine capture (596 wt%), surpassing the most reported amorphous polymer adsorbents. The rich primary amine groups of AHCPs promote the rapid physical capture of iodine from iodine water and iodine vapor. Intrinsic features such as low-cost preparation, good recyclability, as well as excellent performance in iodine capture indicate that the AHCPs can be used as potential candidates for the removal of iodine from radioactive wastewater and gas mixtures.
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- 2022
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36. High-Throughput Preparation and Characterization of ZrMoTaW Refractory Multi-Principal Element Alloy Film
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Qiannan Wang, Hongwang Yang, Xiaojiao Zuo, Yinxiao Wang, and Jiahao Yao
- Subjects
refractory multi-principal element alloys ,high-throughput screening technology ,multi-gradient deposition method ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
In this work, high-throughput screening technology is applied to four-member refractory multi-principal element alloys (RMPEAs) films with high W content. The exploration of refractory metals such as W is strictly limited by the high melting temperature in this work; a multi-gradient deposition method was introduced to overcome this obstacle. By adjusting the power and distance from the target to the sample, component Zr11Mo11Ta25W53 with the best hardening performance was successfully obtained. The uniformity of the material library was analyzed from the perspectives of phase structure and micromorphology. With the help of Hume-Rothery theory and XRD analysis, it is shown that the film has a stable bcc structure. It is believed that film uniformity, nanoscale size, preferential orientation, surface roughness, and solution mechanism are the pivotal factors to improve hardness performance, especially for high W components. The hardness and modulus of elasticity can reach 20 GPa and 300 GPa, respectively, and the H/Er and H3/Er2 values are 0.067 and 0.065, showing the best wear resistance in many samples.
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- 2022
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37. Bending-induced deformation twinning in body-centered cubic tungsten nanowires
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Siyuan Wei, Qiannan Wang, Hua Wei, and Jiangwei Wang
- Subjects
Bending ,body-centered cubic ,twinning ,dislocation ,tungsten nanowire ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
The competition between dislocation slip and deformation twinning in body-centered cubic (BCC) nanocrystals can be strongly influenced by the deformation conditions. In this study, we investigate the deformation of [112]-oriented BCC tungsten nanowires under different loading modes. It shows that dislocation plasticity is a predominant deformation mode under uniaxial tension, while deformation twinning prevails when non-uniaxial stress is applied. The interfaces of bending-induced twinning are composed of numerous stepwise {112} twin boundaries. These findings shed light on the deformation mechanism of BCC nanocrystals under complex loading conditions.
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- 2019
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38. EFFECT OF NANO-TiO₂ ON THE DURABILITY OF ULTRA-HIGH PERFORMANCE CONCRETE WITH AND WITHOUT A FLEXURAL LOAD
- Author
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Chunping Gu, Qiannan Wang, Liu Jintao, and Sun Wei
- Subjects
Ultra-high performance concrete ,Nano-TiO₂ ,Mechanical properties ,Durability ,Flexural load ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
In this study, the durability of nano-TiO₂ modified ultra-high performance concrete (UHPC) with and without a flexural load was experimentally investigated. Firstly, the mechanical properties of UHPC with various nano-TiO₂ contents were tested, and the results showed that UHPC with 1 wt% nano-TiO₂ exhibited the best mechanical properties. Then, 1wt% nano-TiO2 was added into UHPC to evaluate its effects on dry shrinkage, chloride ingress resistance, freeze-thaw resistance and carbonation resistance of UHPC. The effect of the flexural load on the durability of UHPC was also studied. The dry shrinkage of nano-TiO₂ modified UHPC was reduced compared with the control UHPC. The flexural load accelerated the chloride penetration process in the tensile region of the UHPC specimens, and the addition of nano-TiO₂ mitigated the negative influence of flexural load on the chloride ingress resistance of UHPC. Moreover, the addition of nano-TiO2 particles also improved the freeze-thaw resistance of the flexural loaded UHPC by reducing the mass loss under 800 freeze-thaw cycles. Carbonation was not detected in all UHPC specimens after being exposed to 60% CO₂ for 180 days. Furthermore, the MIP results indicated that the addition of nano-TiO₂ refined the pore structure of the UHPC, which improved the mechanical properties and durability of the UHPC.
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- 2018
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39. Curvature domains in V4 of macaque monkey
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Jia Ming Hu, Xue Mei Song, Qiannan Wang, and Anna Wang Roe
- Subjects
V4 ,curvature ,hypercolumn ,optical imaging ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
An important aspect of visual object recognition is the ability to perceive object shape. Two basic components of complex shapes are straight and curved contours. A large body of evidence suggests a modular hierarchy for shape representation progressing from simple and complex orientation in early areas V1 and V2, to increasingly complex stages of curvature representation in V4, TEO, and TE. Here, we reinforce and extend the concept of modular representation. Using intrinsic signal optical imaging in Macaque area V4, we find sub-millimeter sized modules for curvature representation that are organized from low to high curvatures as well as domains with complex curvature preference. We propose a possible ‘curvature hypercolumn’ within V4. In combination with previous studies, we suggest that the key emergent functions at each stage of cortical processing are represented in systematic, modular maps.
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- 2020
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40. Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity in Colletotrichum gloeosporioides
- Author
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Xuesheng Gao, Qiannan Wang, Qingdeng Feng, Bei Zhang, Chaozu He, Hongli Luo, and Bang An
- Subjects
HSF1 ,melanin ,appressorium ,pathogenicity ,Colletotrichum gloeosporioides ,Biology (General) ,QH301-705.5 - Abstract
Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. In this study, we analyze the role of CgHSF1 in the pathogenicity of Colletotrichum gloeosporioides and investigate the underlying mechanism. Failure to generate the Cghsf1 knock-out mutant suggested that the gene is essential for the viability of the fungus. Then, genetic depletion of the Cghsf1 was achieved by inserting the repressive promoter of nitrite reductase gene (PniiA) before its coding sequence. The mutant showed significantly decrease in the pathogenicity repression of appressorium formation, and severe defects in melanin biosynthesis. Moreover, four melanin synthetic genes were identified as direct targets of CgHSF1. Taken together, this work highlights the role of CgHSF1 in fungal pathogenicity via the transcriptional activation of melanin biosynthesis. Our study extends the understanding of fungal HSF1 proteins, especially their involvement in pathogenicity.
- Published
- 2022
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41. Chemokine Receptor 5, a Double-Edged Sword in Metabolic Syndrome and Cardiovascular Disease
- Author
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Zhongwen Zhang, Qiannan Wang, Jinming Yao, Xiaojun Zhou, Junyu Zhao, Xiaoqian Zhang, Jianjun Dong, and Lin Liao
- Subjects
CCR5 ,inflammation ,endothelial dysfunction ,cardiovascular disease ,metabolic syndrome ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The key characteristic of cardiovascular disease (CVD) is endothelial dysfunction, which is likely the consequence of inflammation. It is well demonstrated that chemokines and their receptors play a crucial role in regulating inflammatory responses, and recently, much attention has been paid to chemokine receptor 5 (CCR5) and its ligands. For example, CCR5 aggravates the inflammatory response in adipose tissue by regulating macrophage recruitment and M1/M2 phenotype switch, thus causing insulin resistance and obesity. Inhibition of CCR5 expression reduces the aggregation of pro-atherogenic cytokines to the site of arterial injury. However, targeting CCR5 is not always effective, and emerging evidence has shown that CCR5 facilitates progenitor cell recruitment and promotes vascular endothelial cell repair. In this paper, we provide recent insights into the role of CCR5 and its ligands in metabolic syndrome as related to cardiovascular disease and the opportunities and roadblocks in targeting CCR5 and its ligands.
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- 2020
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42. Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
- Author
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Xiaolu Qu, Qiannan Wang, Haiyan Wang, and Shanjin Huang
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we describe the protocol of revealing and quantifying the spatial organization of actin using fluorescent phalloidin-staining in fixed Arabidopsis pollen grains and pollen tubes. We also introduce the method of assessing the stability and/or turnover rate of actin filaments in pollen cells using the treatment of latrunculin B.
- Published
- 2020
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43. A Relationship Model between Top Management Team Cognitive Heterogeneity and Strategic Decision Quality and Its Implications for Sustainability
- Author
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Qiannan Wang, Yuhui Ge, and Cuihua Hu
- Subjects
Electronic computers. Computer science ,QA75.5-76.95 - Abstract
To achieve sustainable development, a top management team (TMT) and the quality of its strategic decisions on sustainability are critical. This paper builds a relationship model between top management team (TMT) cognitive heterogeneity and the quality of strategic decisions. This research is important because TMT cognitive heterogeneity can comport decision-making schemes with complex and changing environments. Specifically, this paper explores the intermediary role of the team fusion process composed of different shareholders’ agents in integrating TMT heterogeneous cognition to improve the quality of strategic decisions. It adopts the upper echelon theory, which proposes that decision-makers have limited rationality and face difficulty in collecting and analyzing information in complex and changing environments. A questionnaire survey of 107 Chinese enterprises was conducted. Through the research framework of “cognitive heterogeneity–team fusion–strategic decision quality,” this paper constructs a moderated mediator model with entrepreneurial spirit as the moderating variable. Based on statistical analysis, the following results and contributions are obtained. First, TMT cognitive heterogeneity positively affected strategic decision quality. Second, two dimensions of team fusion—information integration and emotional fusion—mediated the relationship between TMT cognitive heterogeneity and strategic decision quality. Third, entrepreneurial spirit positively moderated the relationship between information integration and strategic decision quality, as well as the relationship between emotional fusion and strategic decision quality. The implications of our results related to sustainability are also discussed.
- Published
- 2020
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44. Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development
- Author
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Donghong Chen, Qiannan Wang, Jing Feng, Ying Ruan, and Wen-Hui Shen
- Subjects
ZRF1 ,root meristem ,embryogenesis ,cell layer organization ,cell identity ,Plant culture ,SB1-1110 - Abstract
The H2A/UBIQUITIN-binding proteins AtZRF1a/b have been reported as key regulators involved in multiple processes of Arabidopsis plant growth and development. Yet, the cellular and molecular mechanisms underlying the mutant phenotype remain largely elusive. Here we show that loss-of-function of AtZRF1a/b causes defective root elongation and deformed root apical meristem organization in seedlings. The premature termination of the primary root in the atzrf1a;atzrf1b double mutant is associated with an advanced onset of endoreduplication and subsequent consumption of reservoir stem cells. Cytological analyses using cell type-specific markers and florescent dyes indicate that AtZRF1a/b are involved in maintenance of proper cell layer organization, determinacy of cell identity, and establishment of auxin gradient and maximum at the root tip. During embryogenesis AtZRF1a/b act dominantly in regulating the maintenance of ground tissue initial cells and production of lateral root cap. Lastly, quantitative real-time polymerase chain reaction analysis shows mis-expression of some key genes involved in regulating cell patterning, cell proliferation and/or hormone pathways. Our results provide important insight into AtZRF1a/b function in cell fate determinacy and in establishment and maintenance of proper stem cell reservoir during embryonic and post-embryonic root development.
- Published
- 2019
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45. PREDICTION OF CHLORIDE INGRESS IN STEEL FIBRE REINFORCED CONCRETE UNDER BENDING LOAD
- Author
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Qiannan Wang, Wei Sun, Guo Liping, Gu Chunping, and Zong Jinyu
- Subjects
Steel fibre reinforced concrete ,Chloride ,Time-dependent diffusion coefficient ,Corrosion initiation ,Stress ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
Chloride induced corrosion is an important reason for the deterioration of reinforced concrete structures. The chloride transport properties of steel fibre reinforced concrete (SFRC) coupled with and without bending load (stress level of 0.5) were investigated through bulk diffusion test. A prediction model for chloride ingress in SFRC under bending load was established based on the Fick's second law. The time-dependent chloride diffusion coefficient was discussed and calculated. Apparent chloride diffusion coefficient was used to predict the corrosion initiation of SFRC structures. The experimental results showed that SFRC had better chloride resistance than plain concrete, especially when specimens were under bending load. The chloride diffusion coefficient of SFRC under tension was 30∼38% lower than that of plain concrete. For unstressed concrete, the calculated corrosion initiation of SFRC was 6∼40% longer compared with plain concrete. For concrete under bending load, the corrosion initiation of SFRC was 2.2∼3.6 times of that for plain concrete, varying with fibres dosage and cover thickness.
- Published
- 2017
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46. Privacy-Preserving and Lightweight Selective Aggregation with Fault-Tolerance for Edge Computing-Enhanced IoT
- Author
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Qiannan Wang and Haibing Mu
- Subjects
Internet of Things (IoT) ,edge computing ,selective aggregation ,privacy-preserving ,fault tolerance ,Chemical technology ,TP1-1185 - Abstract
Edge computing has been introduced to the Internet of Things (IoT) to meet the requirements of IoT applications. At the same time, data aggregation is widely used in data processing to reduce the communication overhead and energy consumption in IoT. Most existing schemes aggregate the overall data without filtering. In addition, aggregation schemes also face huge challenges, such as the privacy of the individual IoT device’s data or the fault-tolerant and lightweight requirements of the schemes. In this paper, we present a privacy-preserving and lightweight selective aggregation scheme with fault tolerance (PLSA-FT) for edge computing-enhanced IoT. In PLSA-FT, selective aggregation can be achieved by constructing Boolean responses and numerical responses according to specific query conditions of the cloud center. Furthermore, we modified the basic Paillier homomorphic encryption to guarantee data privacy and support fault tolerance of IoT devices’ malfunctions. An online/offline signature mechanism is utilized to reduce computation costs. The system characteristic analyses prove that the PLSA-FT scheme achieves confidentiality, privacy preservation, source authentication, integrity verification, fault tolerance, and dynamic membership management. Moreover, performance evaluation results show that PLSA-FT is lightweight with low computation costs and communication overheads.
- Published
- 2021
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47. A Numerical Study on Chloride Diffusion in Cracked Concrete
- Author
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Qiannan Wang, Guoshuai Zhang, Yunyun Tong, and Chunping Gu
- Subjects
cracked concrete ,crack width ,crack depth ,tortuosity ,numerical simulation ,Crystallography ,QD901-999 - Abstract
The cracks in concrete are a fast transport path for chlorides and influence the service life of concrete structures in chloride environments. This study aimed to reveal the effect of crack geometry on chloride diffusion in cracked concrete. The chloride diffusion process in cracked concrete was simulated with the finite difference method by solving Fick’s law. The results showed that the apparent chloride diffusivity was lower in more tortuous cracks, and the cracks with more narrow points also showed lower apparent chloride diffusivity. For tortuous cracks, a higher crack width meant relatively more straight cracks, and consequently, higher apparent chloride diffusivity, while a lower crack width resulted in more tortuous cracks and lower apparent chloride diffusivity. The crack depth showed a more significant influence on the chloride penetration depth in cracked concrete than crack geometry did. Compared with rectangular and V-shaped cracks, the chloride diffusion process in cracked concrete with a tortuous crack was slower at the early immersion age. At the same crack depth, the crack geometry showed a marginal influence on the chloride penetration depth in cracked concrete during long-term immersion.
- Published
- 2021
- Full Text
- View/download PDF
48. Prediction on Permeability of Engineered Cementitious Composites
- Author
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Qiannan Wang, Guoshuai Zhang, Yunyun Tong, and Chunping Gu
- Subjects
engineered cementitious composites ,permeability ,model ,porosity ,paste ,Crystallography ,QD901-999 - Abstract
Permeability of concrete is regarded as a basic indicator of its durability. This paper proposed a simple model to predict the permeability of engineered cementitious composites (ECC), which are fiber reinforced cementitious composites with extremely high ductility and toughness. The permeability of cement paste in ECC was firstly determined based on the general effective media theory. The needed microstructure information of cement paste was obtained from a simulated microstructure. Porosity of the interfacial transition zone (ITZ) was obtained with an ITZ porosity model, and then used to calculate the permeability of ITZ. The permeability of the matrix was determined according to the general self-consistent scheme, and the influence of fiber was simplified with its volume fraction. The calculated permeability of ECC was verified with results from water permeability tests and the accuracy of the model was acceptable for cement-based materials.
- Published
- 2021
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49. Integrated Analysis of the Transcriptome and Metabolome Revealed Candidate Genes Involved in GA3-Induced Dormancy Release in Leymus chinensis Seeds
- Author
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Bing Li, Pan Zhang, Fengdan Wang, Ran Li, Jian Liu, Qiannan Wang, Wei Liu, Bo Wang, and Guofu Hu
- Subjects
Leymus chinensis ,seed dormancy ,transcriptome ,metabolome ,GA3 ,dormancy release ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. Here, we performed transcriptomic and metabolomic analysis of hulled and de-hulled seeds of L. chinensis treated with or without GA3 to investigate the changes in gene and metabolites associated with dormancy release induced by GA3. The germination test revealed that the optimum concentration of GA3 for disruption of L. chinensis seed dormancy was 577 μM. A total of 4327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA3, respectively, compared with seeds soaked in sterile water. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in providing carbon skeletons and energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose. This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release induced by GA3 in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies.
- Published
- 2021
- Full Text
- View/download PDF
50. Comprehensive transcriptional and functional analyses of melatonin synthesis genes in cassava reveal their novel role in hypersensitive-like cell death
- Author
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Yunxie Wei, Wei Hu, Qiannan Wang, Wei Liu, Chunjie Wu, Hongqiu Zeng, Yu Yan, Xiaolin Li, Chaozu He, and Haitao Shi
- Subjects
Medicine ,Science - Abstract
Abstract Melatonin is a widely known hormone in animals. Since melatonin was discovered in plants, more and more studies highlight its involvement in a wide range of physiological processes including plant development and stress responses. Many advances have been made in the terms of melatonin-mediated abiotic stress resistance and innate immunity in plants, focusing on model plants such as rice and Arabidopsis. In this study, 7 melatonin synthesis genes were systematically analyzed in cassava. Quantitative real-time PCR showed that all these genes were commonly regulated by melatonin, flg22, Xanthomonas axonopodis pv manihotis (Xam) and hydrogen peroxide (H2O2). Transient expression in Nicotiana benthamiana revealed the subcellular locations and possible roles of these melatonin synthesis genes. Notably, we highlight novel roles of these genes in hypersensitive-like cell death, as confirmed by the results of several physiological parameters. Moreover, transient expression of these genes had significant effects on the transcripts of reactive oxygen species (ROS) accumulation and defense-related genes, and triggered the burst of callose depositions and papillae-associated plant defense, indicating the possible role of them in plant innate immunity. Taken together, this study reveals the comprehensive transcripts and putative roles of melatonin synthesis genes as well as melatonin in immune responses in cassava.
- Published
- 2016
- Full Text
- View/download PDF
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