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355 results on '"Qiao, Lulu"'

9. Spray‐induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Author

Qiao, Lulu, Lan, Chi, Capriotti, Luca, Ah‐Fong, Audrey, Sanchez, Jonatan Nino, Hamby, Rachael, Heller, Jens, Zhao, Hongwei, Glass, N Louise, Judelson, Howard S, Mezzetti, Bruno, Niu, Dongdong, and Jin, Hailing

Subjects
Emerging Infectious Diseases, Infectious Diseases, Genetics, 2.2 Factors relating to the physical environment, Aetiology, Infection, Zero Hunger, Ascomycota, Botrytis, Colletotrichum, Gene Silencing, Plant Diseases, RNA Interference, RNA, Double-Stranded, Rhizoctonia, spray&#8208, induced gene silencing, small RNA, RNA interference, double&#8208, stranded RNA, uptake efficiency, double-stranded RNA, spray-induced gene silencing, Biological Sciences, Technology, Medical and Health Sciences, Biotechnology
Abstract

Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray-Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. We first examined the efficiency of RNA uptake in multiple pathogenic and non-pathogenic fungi, and an oomycete pathogen. We observed efficient double-stranded RNA (dsRNA) uptake in the fungal plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Aspergillus niger and Verticillium dahliae, but no uptake in Colletotrichum gloeosporioides, and weak uptake in a beneficial fungus, Trichoderma virens. For the oomycete plant pathogen, Phytophthora infestans, RNA uptake was limited and varied across different cell types and developmental stages. Topical application of dsRNA targeting virulence-related genes in pathogens with high RNA uptake efficiency significantly inhibited plant disease symptoms, whereas the application of dsRNA in pathogens with low RNA uptake efficiency did not suppress infection. Our results have revealed that dsRNA uptake efficiencies vary across eukaryotic microbe species and cell types. The success of SIGS for plant disease management can largely be determined by the pathogen's RNA uptake efficiency.

Published
2021

26. In Situ Reconstructed Cu/β‐Co(OH)2 Tandem Catalyst for Enhanced Nitrate Electroreduction to Ammonia in Ampere‐Level.

Author

Qiao, Lulu, Zhu, Anquan, Liu, Di, An, Keyu, Feng, Jinxian, Liu, Chunfa, Ng, Kar Wei, and Pan, Hui

Subjects
*ELECTROLYTIC reduction, *COPPER, *REDUCTION potential, *ATOMIC hydrogen, *AMMONIA, *DENITRIFICATION, *NITRITES
Abstract

The electrochemical nitrate reduction for green ammonia production is attracting increasing attention, where the catalysts are widely investigated by controlling the compositions or structures to achieve high performance. However, their reconstructions under reduction potentials are inevitable and uncontrollable, leading to uncertain performance, and a confused understanding of the mechanism. In this work, a strategy is proposed by controlling the pre‐catalyst's reconstruction chemistry toward electrochemical nitrate reduction reaction (e‐NO3RR) with superior activity and stability. To demonstrate the idea, a pre‐catalyst is fabricated with α‐Co(OH)2 and Cu(OH)2 (α‐Co(OH)2/Cu(OH)2), which is in situ reconstructed to a tandem catalyst with Cu and β‐Co(OH)2 (Cu/β‐Co(OH)2) under working potential. Cu/β‐Co(OH)2 achieves an optimal Faraday efficiency for ammonia of 97.7% with a yield rate of 3.9 mmol h−1 cm−2 at −0.5 V, outperforming other reported metal‐hydroxide catalysts. The experimental and theoretical results demonstrate that a tandem catalytic mechanism is responsible for the exceptional performance: 1) Cu functions as the donor of nitrite; and 2) β‐Co(OH)2 serves as active sites for generating active hydrogen and reducing nitrogen‐containing groups. This work highlights that the controllable reconstruction toward improved performance can be realized, and provides an insightful understanding of the mechanism, which is helpful for developing active and stable catalysts for various catalytic applications. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Oxygen vacancy redistribution and ferroelectric polarization relaxation on epitaxial perovskite films during an electrocatalytic process

Author

An, Keyu, primary, Yu, Zhichao, additional, Bai, Haoyun, additional, Liu, Di, additional, Qiao, Lulu, additional, Lv, Xingshuai, additional, Shao, Lianyi, additional, Feng, Jinxian, additional, Cao, Youpeng, additional, Li, Lun, additional, Wen, Zhaorui, additional, Chen, Shi, additional, Pan, Zhongbin, additional, Wang, Shuangpeng, additional, and Pan, Hui, additional

Published
2024
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34. Red Dual-Emissive Carbon Dots for Cu2+ Selective Detection and Dynamical Monitoring.

Author

Pan, Yatao, Xu, Mingyue, Cai, Lin, Qiao, Lulu, Wang, Shihong, Zhang, Kun, Ran, Xia, and Guo, Lijun

Abstract

Monitoring the copper ion (Cu2+) levels has attracted considerable attention due to its significance in environmental and physiological applications. Here, we report on red dual-emission carbon dots (CDs) with a 43% quantum yield and without excitation wavelength dependence for probing Cu2+ in vitro and in vivo. The solid coordination of Cu2+ with the surface groups of CDs generates an asynchronous fluorescent quenching of CDs and enables ultrasensitive detection of Cu2+ in vitro. The intensity ratio of fluorescence at 605 and 650 nm (F605/F650) shows a linear response to Cu2+ concentration ranging from 0 to 1.1 μM, with an ultralow detection sensitivity of 0.58 nM. Moreover, the as-prepared CDs show good stability, anti-interference capability, low cellular toxicity, and exceptional ability to penetrate plant cell walls, endowing the CDs with excellent bioimaging potentials and tracking the dynamic levels of Cu2+ in living cells. [ABSTRACT FROM AUTHOR]

Published
2024
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