Search

Your search keyword '"Qin, Xiangying"' showing total 6 results
Start Over Author "Qin, Xiangying"
6 results on '"Qin, Xiangying"'

6. Stathmin expression in glioma-derived microvascular endothelial cells: a novel therapeutic target.

Author

Dong B, Mu L, Qin X, Qiao W, Liu X, Yang L, Xue L, Rainov NG, and Liu X

Subjects
Adolescent, Adult, Aged, Apoptosis genetics, Cell Movement genetics, Cell Proliferation, Cell Survival genetics, Down-Regulation genetics, Endothelial Cells pathology, Female, Glioma pathology, Humans, Male, Microvessels metabolism, Microvessels pathology, Middle Aged, Neoplasm Invasiveness, Neoplasm Staging, Neovascularization, Pathologic genetics, Neovascularization, Pathologic pathology, RNA, Messenger genetics, RNA, Small Interfering genetics, Stathmin genetics, Young Adult, Endothelial Cells metabolism, Glioma blood supply, Stathmin biosynthesis
Abstract

The purpose of this study was to investigate stathmin expression and its mechanisms of action in GDMEC. Microvascular endothelial cells were isolated from human gliomas (n=68) and normal brain specimans (n=20), and purified by magnetic beads coated with anti-CD105 antibody. The expression of stathmin mRNA and protein were detected by RT-PCR and western blotting, respectively. Stathmin expression was silenced by application of specific siRNA in high grade GDMEC. The proliferation, apoptosis and invasion behavior of GDMEC were investigated. The stathmin positive rate of endothelial cells in normal brain, grade I-II glioma and grade III-IV glioma was 20, 66 and 95.5%, respectively (P<0.05). When cells were treated with siRNA to silence stathmin, cell viability was reduced, the apoptosis rate increased and the migration of vascular endothelial cells was suppressed significantly (P<0.05). Down-regulation of stathmin suppressed neoangiogenesis of glioma and provides a potential target for glioma treatment.

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results