Search

Background: Portal vein thrombosis (PVT) after adult liver transplantation (LT) is a rare but serious complication with no consensus on the ideal treatment. We report a case series and a comprehensive review of the literature on PVT after LT to discuss the therapeutic options. Methods: The clinical data of 360 adult patients (≥18 years of age) who underwent LT from January 2017 to January 2020 were reviewed, and a comprehensive search of PubMed and Web of Science was conducted. Patients diagnosed with PVT after LT were identified, and relevant risk factors and therapies were analyzed. Results: Among the 360 patients, 7 (1.94%) developed PVT after LT. Onset of PVT within one week after LT was found in six patients (85.71%). Four of the seven patients with PVT received systemic anticoagulation (low molecular weight heparin and warfarin) therapy. Minimally invasive interventional therapies combined with systemic anticoagulation (heparin and warfarin) were applied for three patients, two of whom died because of severe abdominal hemorrhage and liver failure. Of the 33 cases reported in the literature, minimally invasive interventional therapy combined with systematic anticoagulation or sclerotherapy were the most-used methods (20/33). Systemic anticoagulation was administered to four patients, and surgical operation (thrombectomy; portosystemic shunt and retransplantation) was performed for nine patients. Among these 33 patients, 4 eventually died. Conclusions: Interventional therapy combined with systemic anticoagulation is a good choice for the management of PVT after LT, and in our experience, systemic anticoagulation alone can also have a positive effect for early PVT patients.

The aim of the present study was to identify the appropriate DNA sequence and design high-quality primers for methylation-specific polymerase chain reaction (MSP). These primers may be used to examine and identify patients with early-stage epithelial ovarian carcinoma (EOC). Opioid binding protein/cell adhesion molecule like (OPCML), Runt-related transcription factor 3 and tissue factor pathway inhibitor 2 were selected as possible molecular markers. MSP primer sets were designed to monitor the methylation of the three markers. Free circulating DNA (fcDNA) from 194 patients with epithelial ovarian carcinoma and healthy donors were templates in the nested MSP. OPCML MSP was effective with respect to screening methylated fcDNA. One-way ANOVA P-values indicated that the difference in cancer antigen 125 (CA125), a biomarker for EOC diagnosis, level between early EOC and healthy donors was not significant. The methylation of OPCML was significantly altered in early-stage EOC compared with healthy donors (P

The urban underground pipe network plays a vital role in the city development, the construction of unreasonable urban pipe networks restricts the city development, even has a disastrous effect on the city. However, it is not desirable to overstate the investment of the urban underground pipe network excessively. It is an important subject of the city construction that how to establish reasonable urban pipe networks to meet service conditions of underground pipe networks. Because the construction of underground drainage pipe networks of most of the major cities in our country mainly comes from the 50s of last century and there are more pipe network system with the development of the city, it is the main content of our research that how to reform and construct them under the condition of existing underground pipe networks.

The influence of length of chord and depth of truss on displacement and bearing capacity of truss construction platform was evaluated based on the results of the finite element analysis on eight different diameters of the top steel-tube truss construction platform of silo. The bearing capacity increases with increasing of depth of truss. The bearing capacity increases with decreasing of length of chord. The maximal displacement increases with increasing of the diameter in the same joint loads, and the displacement curve of model fits better.

The influence of diameters and loads on the displacement of truss supporting system is evaluated based on the results of the finite element analysis on the steel-tube truss supporting system of silo. The vertical displacement increases with the increasing of the load. The displacement increases with the increasing of the diameter in the same loads. The maximal displacement appears in the intermediate position of the truss, and the displacements of symmetric nodes are similar under the symmetric loads. The member yields before 30m of truss supporting system reaches the allowable deflection.

With the development of society, the demand for mining resources is increasing. Associated with underground mining of mining resources, inhomogeneous deformation such as lever tension, compression, tilt, curvature of the surface has been aroused, which generate additional forces to the building foundations, causing damage and destruction to the surface housing. This paper describes the impact on surface buildings by the surface movement and deformation due to underground mining, explores the anti-mining structural system of the surface housing, the structural design theory and the anti-mining reinforcement of new technologies. The aim is to make the building more effectively absorb and resist surface movement and deformation. The measures and recommendations of mine surface housing design and reinforcement are proposed.

Based on the problem that tilt and convergence of some cylindrical structures in the building regularly need monitoring after the construction is completed. 3 D laser scan technology was applied and 3 D laser scanner was used to get the point-cloud data of a cylindrical structure which was deformed for external extrusion in the course of construction. The slope of the structure was calculated by the method of calculating the elliptical circle through more adjustment and proved that the method of using the 3 D laser scan technology to monitor the tilt and convergence of some cylindrical structures in the building is feasible and stable. 3 D laser scan technology can be used to calculate the slope of the cylindrical structure with minor error.

Objective:To study the effect of hesperidin a nd rutin on oxidative modification of high density lipoprotein (HDL) in vitro .Methods:HDL was isolated from healthy human plasma by sequen tial ultracentrifugation, and was oxidized by copper ions. The inhibitory effect s of hesperidin and rutin on HDL oxidative modification were valued by the forma tion of malondialdehyde (MDA). Results:Hesperidin and rutin sig nificantly inhibited copper-induced oxidation of HDL in a dose-dependent manne r.Conclusion: Both hesperidin and rutin can prevent HDL from cop per-induced oxidative modification in vitro. This result suggests that they might have antiatherogenic effect.

To identify the specific protein interactions involved in Bat3-mediated apoptosis.Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.TAP studies showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.TAP was successfully established and is suitable for isolating the binding partners of Bat3.

The present study was aimed to investigate whether Bcl-2, Fas and Bax are involved in monocyte chemotacitic protein-1 (MCP-1)-induced apoptosis of human umbilical vein endothelial cells (hUVECs). hUVECs were cultured, and the purity was identified by immunofluorescence and immunohistochemistry with specific anti-von Willebrand factor (vWF) and anti-VEGF receptor-2 (KDR) antibodies. With 90% confluence hUVECs were serum-starved for 12 h, and then treated with different concentrations of MCP-1 (0.1, 1.0, 10, 100 ng/mL) for 24 and 48 h respectively. The expressions of apoptosis related proteins Fas, Bcl-2, Bax were detected by flow cytometry (FACS) and Western blot. As shown in our preliminary study, MCP-1 induced apoptosis of hUVECs in a dose-dependent manner at both 24 h and 48 h. FACS and Western blot analysis results in the present study indicated that MCP-1 promoted the expression of proapoptotic proteins Bax and Fas and inhibited the expression of antiapoptotic protein Bcl-2. These results suggest that MCP-1 may induce the apoptosis of hUVECs through evoking the imbalance between proapoptotic Fas/Bax and antiapoptotic Bcl-2 protein.