63 results on '"Qing-Quan Zu"'
Search Results
2. Low Psoas-Muscle index is associated with decreased survival in hepatocellular carcinoma treated with transarterial chemoembolization
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Jin-Xing Zhang, Hai-Tao Yan, Ye Ding, Jin Liu, Sheng Liu, Qing-Quan Zu, and Hai-Bin Shi
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Carcinoma ,hepatocellular ,chemoembolization ,therapeutic ,sarcopenia ,survival analysis ,Medicine - Abstract
Purpose Skeletal muscle index (SMI) is a promising predictor of clinical outcomes in patients with malignant diseases. As a simpler surrogate of sarcopenia-psoas muscle index (PMI), its predict value for overall survival (OS) after transarterial chemoembolization (TACE) for hepatocellular carcinoma (HCC) has not been reported. To determine if changes in the PMI predicted OS in individuals with HCC treated with TACE.Patients and Methods A retrospective analysis was performed in HCC patients treated with TACE between January 2018 and March 2019. The survival curve according to PMI was estimated by the Kaplan–Meier method and then compared by the log-rank test. Cox proportional hazards models were conducted to identify the prognostic factors for OS. Furthermore, the predictive abilities of PMI and SMI were compared by using Harrell’s concordance index (C-index).Results Two hundred and twenty-eight patients (175 men, mean age 59 ± 11 years) were analysed. The OS was less in patients with low PMI than those with high PMI (median OS: 16.9 vs. 38.5 months, p
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- 2022
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3. Bronchial artery embolization for the management of frequent hemoptysis caused by bronchiectasis
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Guang-Dong Lu, Hai-Tao Yan, Jin-Xing Zhang, Sheng Liu, Hai-Bin Shi, and Qing-Quan Zu
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Bronchial arteries ,Bronchiectasis ,Embolization ,Therapeutic ,Hemoptysis ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background To retrospectively evaluate the effectiveness of bronchial artery embolization (BAE) compared with conservative therapy for the treatment of frequent hemoptysis caused by bronchiectasis. Methods From January 2015 to December 2019, consecutive patients who were admitted due to frequent (more than three times per year) bronchiectasis-related hemoptysis were retrospectively reviewed. Those who were treated with either BAE (n = 69) or conservative therapy (n = 47) were enrolled for analysis. The technical success, clinical success, and complications of the BAE procedure were evaluated. Long-term hemoptysis-free survival rates and clinical success were compared between patients in the BAE group and patients in the conservative group. A Cox proportional hazard regression model was used to identify the predictors of recurrent hemoptysis. Results The technical success rate was 100% for the BAE procedure, and clinical success was achieved in 92.8% (64 of 69) of cases. No major procedure-related complications occurred, and minor complications were observed in 16 cases (23.2%). The 1-, 2-, and 3-year hemoptysis-free survival rates were 88.3, 71.3, and 66.2%, respectively, for the BAE group and 31.9, 17.6, and 2.5%, respectively, for the conservative treatment group (P
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- 2022
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4. Development of a model to predict recurrence after bronchial artery embolization for non-cancer related hemoptysis
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Hai-Tao Yan, Guang-Dong Lu, Xiang-Zhong Huang, Da-Zhong Zhang, Kun-Yuan Ge, Jin-Xing Zhang, Jin Liu, Sheng Liu, Hai-Bin Shi, and Qing-Quan Zu
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Bronchial arteries ,Embolization ,Therapeutic ,Hemoptysis ,Nomograms ,Recurrence ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Relapse after effective bronchial arterial embolization (BAE) for controlling hemoptysis is not uncommon. Studies reported diverse predictors of recurrence. However, a model to assess the probability of recurrence in non-cancer related hemoptysis patients after BAE has not been reported. This study was to develop a model to predict recurrence after BAE for non-cancer related hemoptysis. Methods The study cohort included 487 patients who underwent BAE for non-cancer-related hemoptysis between January 2015 and December 2019. We derived the model’s variables from univariate and multivariate Cox regression analyses. The model presented as a nomogram scaled by the proportional regression coefficient of each predictor. Model performance was assessed with respect to discrimination and calibration. Results One-month and 1-, 2-, 3- and 5-year recurrence-free rates were 94.5%, 88.0%, 81.4%, 76.2% and 73.8%, respectively. Risk factors for recurrence were underlying lung diseases and the presence of systemic arterial-pulmonary circulation shunts. This risk prediction model with two risk factors provided good discrimination (area under curve, 0.69; 95% confidence interval, 0.62–0.76), and lower prediction error (integrated Brier score, 0.143). Conclusion The proposed model based on routinely available clinical and imaging features demonstrates good performance for predicting recurrence of non-cancer-related hemoptysis after BAE. The model may assist clinicians in identifying higher-risk patients to improve the long-term efficacy of BAE.
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- 2021
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5. Treatment strategies and prognosis for initially unresectable ruptured hepatocellular carcinoma: a single-center experience in 94 patients
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Chun Zhou, Qing-Quan Zu, Xing-Long Liu, Bin Wang, Chun-Gao Zhou, Hai-Bin Shi, and Sheng Liu
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Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
PURPOSE:To identify the treatment options and prognostic factors for patients with initially unresectable ruptured hepatocellular carcinoma (HCC).METHODS:Between June 2012 to December 2016, 94 consecutive patients with initially unresectable ruptured HCC were analyzed retrospectively in this study. Patients were followed until December 2017. Predictors of short-term (≤30 days) and long-term (>30 days) survival were identified by using logistic regression model and Cox proportional hazard model, respectively.RESULTS:Of the 94 patients, initial hemostasis treatment was achieved by transarterial embolization (TAE) in 59 patients, surgical hemostasis in 14, and conservative treatment in 21. Twenty-five (26.6%) patients died within 30 d after tumor rupture. In the multivariate analysis, patients treated with aggressive initial treatment strategies (TAE or surgical hemostasis) (P < 0.001) or those with better Child-Pugh class (P = 0.003) and absence of shock on admission (P = 0.001) had a better chance of short-term survival. Of the 69 patients who survived more than 30 days after initial treatment, the median survival time was 268 d. In the multivariate analysis, among the 69 who survived, early modified LCSGJ stage (P = 0.003) and staged hepatectomy as definitive treatment (P < 0.001) were significant predictors of increased long-term survival.CONCLUSION:Short-term survival of patients with initially unresectable ruptured HCC could achieve with better Child-Pugh class, absence of shock and aggressive initial treatment strategies. After survived the emergency phase of tumor rupture, long-term survival was significantly increased with early modified LCSGJ stage and staged hepatectomy therapy.
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- 2020
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6. Efficacy and Safety of the Combination of Transarterial Chemoembolization with Camrelizumab plus Apatinib for Advanced Hepatocellular Carcinoma: A Retrospective Study of 38 Patients from a Single Center
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Jin-Xing Zhang, Yu-Xing Chen, Chun-Gao Zhou, Jin Liu, Sheng Liu, Hai-Bin Shi, and Qing-Quan Zu
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Objective. To evaluate the effectiveness and safety of transarterial chemoembolization (TACE) combined with immune checkpoint inhibition (camrelizumab) plus an antiangiogenic agent (apatinib) for advanced hepatocellular carcinoma (HCC). Methods. Between March 2019 and April 2021, the clinical data of 38 patients diagnosed with advanced HCC who initially received TACE combined with camrelizumab plus apatinib were reviewed retrospectively. The objective response rate (ORR) and disease control rate (DCR) according to modified response evaluation criteria in solid tumors, progression-free survival (PFS), overall survival (OS), and adverse events (AEs) were evaluated. Results. At 2-3 months after initial therapy, the ORR and DCR was 50.0% (19/38) and 76.3% (29/38), respectively. The median PFS and OS were 7.3 months (range: 1.0–22.6 months) and 13.5 months (range: 2.3–24.3 months), respectively. Treatment-related AEs (grades 3-4) were observed in 25 patients (67.8%). No treatment-related deaths occurred. Conclusion. The combination of TACE with camrelizumab plus apatinib for the treatment of patients with advanced HCC showed promising efficacy and a manageable safety profile.
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- 2022
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7. Differences in efficacy of uncovered self-expandable metal stent in relation to placement in the management of malignant distal biliary obstruction
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Jin-Xing Zhang, Qing-Quan Zu, Sheng Liu, Chun-Gao Zhou, Jin-Guo Xia, and Hai-Bin Shi
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Distal biliary obstruction ,metal stent ,outcome ,sphincter of Oddi ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background/Aims: Metal stent insertion is a common palliative treatment for distal malignant biliary obstruction (MBO) but whether placement across the sphincter of Oddi (SO) causes more complications or shorter survival is in question. The aim of this study was to compare the clinical outcomes of percutaneous uncovered self-expandable metal stent placement above and across the SO in patients with distal MBO. Patients and Methods: We retrospectively studied 59 patients who underwent uncovered metal stent placement for distal MBO between January 2012 and March 2016. Stents were placed above the SO for 22 subjects (group A) and across the SO for 37 subjects (group B). Early cholangitis, stent occlusion, and overall survival time were compared between the two groups. Results: Clinical success was 90.9 and 86.5% for groups A and B, respectively. Early cholangitis occurred in 2 patients (9.1%) in group A and in 6 patients (16.2%) in group B (P = 0.645). Median stent patency and median survival time was 105 and 140 days for group A, 120 and 160 days for group B, respectively. The stent occlusion (31.8% in group A and 21.6% in group B, P = 0.454) and the cumulative stent patency times (P = 0.886) did not differ between the two groups. There was no significant difference in cumulative patient survival between the two groups (P = 0.810). Conclusion: Uncovered metal stent placement across the SO did not significantly affect early cholangitis, stent patency, or patient survival.
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- 2018
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8. A Canine Model of Hemorrhagic Transformation Using Recombinant Tissue Plasminogen Activator Administration After Acute Ischemic Stroke
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Run-Hao Jiang, Qing-Quan Zu, Xiao-Quan Xu, Bin Wang, Ye Ding, Jun Wang, Sheng Liu, and Hai-Bin Shi
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blood-brain barrier ,canines ,hemorrhagic transformation ,ischemic stroke ,MMP-9 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Early reperfusion of occluded arteries via recombinant tissue plasminogen activator (rtPA) administration is considered to be an effective strategy for the treatment of acute ischemic stroke. However, delayed administration of rtPA may cause severe hemorrhagic transformation (HT) and undesirable neurological outcomes. The current study aims to establish a canine HT model using rtPA administration and to investigate the potential mechanisms underlying HT. Following anesthesia, two autologous clots were injected into the middle cerebral artery (MCA) to induce ischemic stroke. To induce reperfusion, rtPA (2 mg/kg) was administrated intravenously 4.5 h after the establishment of stroke. The occurrence of HT was determined by computed tomography (CT) and by pathological assessment. Transmission electron microscopy was utilized to assess blood-brain barrier (BBB) damage. The expression of matrix metalloprotein 9 (MMP-9) was analyzed by enzyme linked immunosorbent assay (ELISA), immunofluorescence (IF), and western blot. Administration of rtPA 4.5 h after stroke induced reperfusion in 73.9% of the canines, caused evident HT, and did not improve neurological outcomes compared to canines that did not receive rtPA. There was a significant increase in expression of MMP-9 after rtPA administration, accompanied by BBB disruption. We have established a canine HT model that closely mimics human HT by using rtPA administration after the induction of middle cerebral artery occlusion (MCAO) with autologous clots. Our data suggest that a potential mechanism underlying rtPA-caused HT may be related to BBB dysfunction induced by an increase in MMP-9 expression.
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- 2019
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9. In vivo MR imaging of intraarterially delivered magnetically labeled mesenchymal stem cells in a canine stroke model.
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Shan-Shan Lu, Sheng Liu, Qing-Quan Zu, Xiao-Quan Xu, Jing Yu, Jian-Wei Wang, Yu Zhang, and Hai-Bin Shi
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Medicine ,Science - Abstract
BACKGROUND: This study aimed to evaluate the feasibility of intraarterial (IA) delivery and in vivo MR imaging of superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) in a canine stroke model. METHODOLOGY: MSCs harvested from beagles' bone marrow were labeled with home-synthesized SPIO. Adult beagle dogs (n = 12) were subjected to left proximal middle cerebral artery (MCA) occlusion by autologous thrombus, followed by two-hour left internal carotid artery (ICA) occlusion with 5 French vertebral catheter. One week later, dogs were classified as three groups before transplantation: group A: complete MCA recanalization, group B: incomplete MCA recanalization, group C: no MCA recanalization. 3×10(6) labeled-MSCs were delivered through left ICA. Series in vivo MRI images were obtained before cell grafting, one and 24 hours after transplantation and weekly thereafter until four weeks. MRI findings were compared with histological studies at the time point of 24 hours and four weeks. PRINCIPAL FINDINGS: Home-synthesized SPIO was useful to label MSCs without cell viability compromise. MSCs scattered widely in the left cerebral hemisphere in group A, while fewer grafted cells were observed in group B and no cell was detected in group C at one hour after transplantation. A larger infarction on the day of cell transplantation was associated with more grafted cells in the brain. Grafted MSCs could be tracked effectively by MRI within four weeks and were found in peri-infarction area by Prussian blue staining. CONCLUSION: It is feasible of IA MSCs transplantation in a canine stroke model. Both the ipsilateral MCA condition and infarction volume before transplantation may affect the amount of grafted cells in target brain. In vivo MR imaging is useful for tracking IA delivered MSCs after SPIO labeling.
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- 2013
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10. Comparison of Bronchial Artery Embolisation Versus Conservative Treatment for Bronchiectasis-Related Nonmassive Haemoptysis: A Single-Centre Retrospective Study
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Hai-Tao Yan, Guang-Dong Lu, Jin-Xing Zhang, Chun-Gao Zhou, Jin Liu, Sheng Liu, Hai-Bin Shi, and Qing-Quan Zu
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Radiology, Nuclear Medicine and imaging ,Cardiology and Cardiovascular Medicine - Published
- 2023
11. Non-negligible clinical relevance of haemorrhagic transformation after endovascular thrombectomy with successful reperfusion in acute ischaemic stroke
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Xiao Quan Xu, J. Liu, Yong Wang, Kai Qiu, Qing-Quan Zu, Song Liu, Hongwei Shi, and Lin-Bo Zhao
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Male ,medicine.medical_specialty ,Infarction ,Gastroenterology ,Stroke onset ,Text mining ,Modified Rankin Scale ,Internal medicine ,Occlusion ,Ischaemic stroke ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Clinical significance ,Aged ,Cerebral Hemorrhage ,Ischemic Stroke ,Retrospective Studies ,Thrombectomy ,business.industry ,Endovascular Procedures ,Retrospective cohort study ,General Medicine ,medicine.disease ,Treatment Outcome ,Reperfusion ,Female ,Tomography, X-Ray Computed ,business - Abstract
AIM To explore the association between haemorrhagic transformation (HT) subtypes and functional outcome in acute ischaemic stroke (AIS) patients with successful recanalization treated by endovascular thrombectomy (EVT). MATERIALS and METHODS Consecutive patients with AIS due to large-vessel occlusion in the anterior circulation, who were treated between January 2015 and June 2019 and achieved successful EVT, were enrolled in this retrospective study. HT was categorized according to the Heidelberg Bleeding Classification. Functional outcome was evaluated using the 90-day modified Rankin Scale (mRS) after stroke onset. Ordinal logistic regression analysis was performed to determine the association of HT subtypes with functional outcomes. RESULTS A total of 243 patients were included for further analysis. Among them, 121 (49.8%) had HT. Ten (4.1%) patients were classified as haemorrhagic infarction (HI) subtype 1, 61 (25.1%) as HI subtype 2, 17 (7.0%) as parenchymal haematoma (PH) subtype 1, and 33 (13.6%) as PH subtype 2. Ordinal logistic regression analysis suggested that HI subtype 2 (adjusted common OR 0.357, 95% CI: 0.192–0.667), PH1 (adjusted common OR 0.254, 95% CI: 0.093–0.696) and PH subtype 2 (adjusted common OR 0.017, 95% CI: 0.006–0.051) were significantly associated with poor functional outcomes. CONCLUSION The present study shows that HI subtype 2, PH subtype 1, and PH subtype 2 are independently associated with poor clinical outcomes in AIS patients with successful recanalization after EVT.
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- 2022
12. Comparison of the Efficacy and Safety of Transarterial Chemoembolization with or without Lenvatinib for Unresectable Hepatocellular Carcinoma: A Retrospective Propensity Score–Matched Analysis
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Yu-Xing Chen, Jin-Xing Zhang, Chun-Gao Zhou, Jin Liu, Sheng Liu, Hai-Bin Shi, and Qing-Quan Zu
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Journal of Hepatocellular Carcinoma - Abstract
Yu-Xing Chen,1,* Jin-Xing Zhang,1,* Chun-Gao Zhou,1 Jin Liu,2 Sheng Liu,1 Hai-Bin Shi,1 Qing-Quan Zu1 1Department of Interventional Radiology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, Peopleâs Republic of China; 2Department of Clinical Medicine Research Institution, The First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Qing-Quan Zu, Department of Interventional Radiology, The First Affiliated Hospital with Nanjing Medical University, No. 300 Guangzhou Road, Nanjing, 210029, Peopleâs Republic of China, Tel +86-25-68306316 ; +86-13913807470, Fax +86-25-83724440, Email zuqingquan@njmu.edu.cnBackground: Combination of angiogenesis inhibitor may achieve better therapeutic synergistic efficacy, considering of tumor hypoxia and promoted angiogenesis after transarterial chemoembolization (TACE). This study aimed to compare the safety and efficacy of TACE plus lenvatinib (TACE-lenvatinib) with TACE alone for patients with unresectable hepatocellular carcinoma (HCC).Methods: Between June 2019 and September 2021, a total of 215 patients diagnosed with unresectable HCC were retrospectively reviewed, including 53 patients who received TACE-lenvatinib and 162 patients who received TACE alone. The patient selection bias between the TACE-lenvatinib group and the TACE group was balanced by propensity score matching analysis at a 1:2 ratio. Progression-free survival (PFS), overall survival (OS) and tumor response were evaluated in the two groups.Results: After propensity score matching analysis, 34 patients receiving TACE-lenvatinib and 68 patients receiving TACE alone were enrolled. The median PFS and OS times in the TACE-lenvatinib group were significantly greater than those in the TACE group (PFS: 8.3 months vs 4.6 months, P = 0.008; OS: 27.7 months vs 18.4 months, P = 0.043). The objective response rate (ORR) in the TACE-lenvatinib group was higher than that in the TACE alone group (64.1% vs 36.5%, P = 0.002). Univariate and multivariate analyses revealed that TACE-lenvatinib treatment was an independent favorable prognostic factor for both PFS and OS.Conclusion: For unresectable HCC patients, the TACE-lenvatinib appeared superior to TACE alone regarding tumor control, PFS, and OS. However, considering the limitations of this study, these results should be interpreted as preliminary and warrant further confirmation.Keywords: carcinoma, hepatocellular, chemoembolization, therapeutic, lenvatinib, survival analysis, propensity score
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- 2022
13. Risk factor for vital signs elevation during percutaneous microwave ablation of liver tumor under deep sedation
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Qing-Quan Zu, Wei-Zhong Zhou, Sheng Liu, Wen-Tao Wu, Zhen-Yu Jia, Yu Chen, Hai-Bin Shi, and Bin Leng
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medicine.medical_specialty ,Respiratory rate ,Sedation ,Vital signs ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Heart rate ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Risk factor ,Microwaves ,Retrospective Studies ,Univariate analysis ,Vital Signs ,business.industry ,Liver Neoplasms ,Microwave ablation ,Middle Aged ,Treatment Outcome ,030220 oncology & carcinogenesis ,Catheter Ablation ,Cardiology ,Deep Sedation ,medicine.symptom ,Propofol ,business ,medicine.drug - Abstract
To evaluate the frequency and the degree of vital signs elevation, as well as to elucidate the risk factors for elevation of cardiopulmonary parameters. We retrospectively evaluated the medical records of 101 patients who received microwave ablation (MWA) under deep sedation with propofol. Univariate analysis followed by multivariate linear regression analysis was performed to determine the risk factors associated with the elevation of cardiopulmonary parameters. The heart rate (HR), mean blood pressure (BP) and respiratory rate (RR) were elevated in 53.5%, 45.5% and 30.7%. Hyperhemodynamic state (mean BP or HR increased > 30% of the baseline) and high RR (RR > 20 times/min) were detected in 23.8% and 13.9%. Age ≤ 50 years was signifiant for mean BP and HR elevation (p = 0.032; p = 0.027), ablation zone abutting the parietal peritoneum (p = 0.001; p = 0.001; p
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- 2021
14. Transarterial chemoembolization combined with lenvatinib versus transarterial chemoembolization combined with sorafenib for unresectable hepatocellular carcinoma: A comparative retrospective study
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Jin‐Xing Zhang, Yu‐Xing Chen, Chun‐Gao Zhou, Jin Liu, Sheng Liu, Hai‐Bin Shi, and Qing‐Quan Zu
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Infectious Diseases ,Hepatology - Abstract
Tyrosine kinase inhibitors target transarterial chemoembolization (TACE)-mediated vascular endothelial growth factor to inhibit tumor revascularization and to slow tumor progression. The present study aimed to compare the clinical outcomes of TACE combined with lenvatinib (TACE-lenvatinib) and TACE combined with sorafenib (TACE-sorafenib) in patients with unresectable hepatocellular carcinoma (HCC).The clinical data of patients diagnosed with unresectable HCC who received TACE-lenvatinib or TACE-sorafenib between January 2018 and April 2021 were retrospectively reviewed. The tumor response, progression-free survival (PFS), overall survival (OS), and adverse events (AEs) were evaluated and compared between the two groups.A total of 112 patients were enrolled and classified into the TACE-lenvatinib group (n = 53) and the TACE-sorafenib group (n = 59). The objective response rates of patients in the TACE-lenvatinib and TACE-sorafenib groups were 54.7% and 44.1%, respectively (p = 0.260), and the disease control rates (DCRs) were 81.1% and 61.0% (p = 0.020). The median PFS time was significantly longer in the TACE-lenvatinib group than in the TACE-sorafenib group (10.7 vs. 6.0 months; p = 0.002). The median OS time between the TACE-lenvatinib and TACE-sorafenib groups also showed a significant difference (30.5 vs. 20.5 months, p = 0.018). All treatment-related AEs and grade 3/4 AEs were comparable between the two groups (p 0.05).Compared to TACE-sorafenib, TACE-lenvatinib was associated with better DCR, PFS and OS outcomes in patients with unresectable HCC. In subgroups of Barcelona Clinic Liver Cancer B stage or TACE-refractory patients, TACE-lenvatinib also showed a trend of superiority.
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- 2022
15. Association of thrombus histopathology with mechanical thrombectomy: an in vitro study tested by thromboelastography and magnetic resonance imaging
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Xing-Long Liu, Bin Wang, Qing-Quan Zu, Hai-Bin Shi, and Sheng Liu
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circulatory and respiratory physiology - Abstract
Objective: To detect the association between the content of red blood cell (RBC) within thrombi and thromboelastography, magnetic resonance imaging and retrieving strength during thrombectomy in vitro.Methods: Arterial and venous blood samples, considered as RBC-rich groups, were collected from twenty healthy beagle dogs under general anesthesia. RBCs were diminished by gathering the plasma and buffy coat layer after centrifugation. Then samples were grouped as: arterial whole blood group, venous whole blood group, arterial plasma group and venous plasma group. Blood routine test and coagulation test were used to quantify the content of RBC, platelet and fibrin. After tested by thromboelastography, these four groups of blood samples were proceed to clots and compared by magnetic resonance imaging (MRI), HE staining and mechanical thrombectomy in vitro. Results: TEG assay showed MA of whole blood groups with high RBCs was significantly higher than that of plasma groups. MRI demonstrated the plasma clots were much more homogeneous than whole blood samples. T2 value of plasma clots on GRE T2*MR imaging sequence were higher than that of whole blood groups (PConclusion: RBC-rich clots showed lower T2 value, higher retrieving strength but more fragile during mechanical thrombectomy in vitro compared with those of plasma clots. Further, the retrieving strength was positively correlated with MA.
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- 2022
16. Skeletal-muscle index predicts survival after percutaneous transhepatic biliary drainage for obstructive jaundice due to perihilar cholangiocarcinoma
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Hai-Tao Yan, Qing-Quan Zu, Hai-Bin Shi, Chun-Gao Zhou, Jin Liu, Ye Ding, Sheng Liu, and Jin-Xing Zhang
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Male ,medicine.medical_specialty ,education ,030230 surgery ,Gastroenterology ,Cholangiocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Perihilar Cholangiocarcinoma ,Muscle, Skeletal ,Survival analysis ,Aged ,Retrospective Studies ,business.industry ,Hazard ratio ,Cancer ,Middle Aged ,Hepatology ,medicine.disease ,Confidence interval ,Jaundice, Obstructive ,Bile Duct Neoplasms ,Sarcopenia ,Drainage ,030211 gastroenterology & hepatology ,Surgery ,business ,Klatskin Tumor ,Abdominal surgery - Abstract
Sarcopenia is emerging as a prognostic factor in patients with malignant diseases. The prognostication of perihilar cholangiocarcinoma (PHC) with obstructive jaundice was complex, because these patients suffered compete mortality events beyond cancer itself. Our study was to investigate the association between low skeletal-muscle index and overall survival (OS) after percutaneous transhepatic biliary drainage (PTBD) for obstructive jaundice due to PHC. We performed a retrospective survival analysis of patients undergoing PTBD for PHC-related obstructive jaundice between January 2016 and March 2019. Using computed tomography, we measured skeletal-muscle mass at the third lumbar vertebra (L3) to obtain a skeletal-muscle index (SMI). Then, we compared OS between low- and high-SMI groups. Furthermore, factors that could potentially affect OS were assessed. One hundred and four patients (56 males; mean age 66 ± 12 years) were analyzed. Median OS after PTBD was 150 days. OS was shorter in patients with low SMI than in those with high SMI (median OS, 120 vs. 270 days; P
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- 2020
17. Emergency transarterial embolization followed by staged hepatectomy versus emergency hepatectomy for ruptured hepatocellular carcinoma: a single-center, propensity score matched analysis
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Qing-Quan Zu, Sheng Liu, Bin Wang, Hai-Bin Shi, Chu Zhang, Chun-Gao Zhou, and Chun Zhou
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Adult ,Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Blood transfusion ,medicine.medical_treatment ,Single Center ,Disease-Free Survival ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Transarterial embolization ,medicine ,Hepatectomy ,Humans ,Radiology, Nuclear Medicine and imaging ,Propensity Score ,Aged ,Retrospective Studies ,Rupture, Spontaneous ,business.industry ,Liver Neoplasms ,Middle Aged ,medicine.disease ,Combined Modality Therapy ,Embolization, Therapeutic ,Surgery ,Perioperative blood loss ,Radiation therapy ,Treatment Outcome ,Liver ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Propensity score matching ,Feasibility Studies ,Female ,Emergencies ,business - Abstract
To compare the feasibility and efficacy of emergency transarterial embolization (TAE) followed by staged hepatectomy (SH) with emergency hepatectomy (EH) for ruptured hepatocellular carcinoma (HCC). Between January 2012 and December 2017, 102 patients with HCC rupture received EH or emergency TAE followed by SH in our center. Patients were followed until April 2019. Propensity score matching (PSM) analysis was used at a 1:2 ratio, resulting in 20 patients in the SH group and 40 patients in the EH group. We retrospectively compared the operative variables, recurrence status, disease-free survival (DFS), and overall survival (OS) of patients between the two matched groups. Compared with the matched EH group, the SH group showed significantly decreased perioperative blood loss or blood transfusion, shortened intraoperative duration of clamping and postoperative hospital stay (P
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- 2020
18. Treatment strategies and prognosis for initially unresectable ruptured hepatocellular carcinoma: a single-center experience in 94 patients
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Bin Wang, Chun-Gao Zhou, Xing-Long Liu, Chun Zhou, Hai-Bin Shi, Qing-Quan Zu, and Sheng Liu
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Adult ,Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,medicine.medical_treatment ,Single Center ,Interventional Radiology ,medicine ,Carcinoma ,Hepatectomy ,Humans ,Radiology, Nuclear Medicine and imaging ,Embolization ,Survival analysis ,Aged ,Retrospective Studies ,Rupture ,Proportional hazards model ,business.industry ,Liver Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,Embolization, Therapeutic ,Magnetic Resonance Imaging ,Survival Analysis ,Hemostasis, Surgical ,Surgery ,Treatment Outcome ,Hemostasis ,Hepatocellular carcinoma ,Multivariate Analysis ,Female ,Tomography, X-Ray Computed ,Cardiology and Cardiovascular Medicine ,business - Abstract
PURPOSE: We aimed to identify the treatment options and prognostic factors for patients with initially unresectable ruptured hepatocellular carcinoma (HCC). METHODS: Between June 2012 and December 2016, 94 consecutive patients with initially unresectable ruptured HCC were analyzed retrospectively. Patients were followed until December 2017. Predictors of short-term (≤30 days) and long-term (>30 days) survival were identified by using logistic regression model and Cox proportional hazard model, respectively. RESULTS: Of the 94 patients, initial hemostasis was achieved by transarterial embolization (TAE) in 59 patients, surgical hemostasis in 14 patients, and conservative treatment in 21 patients. Twenty-five (26.6%) patients died within 30 days of tumor rupture. In the multivariate analysis, patients treated with aggressive initial treatment strategies (TAE or surgical hemostasis) (P < 0.001) or those with better Child-Pugh class (P = 0.003) and absence of shock on admission (P = 0.001) had a better chance of short-term survival. For 69 patients who survived more than 30 days after initial treatment, the median survival time was 268 days. In the multivariate analysis, among the 69 who survived, early modified LCSGJ stage (P = 0.003) and staged hepatectomy as definitive treatment (P < 0.001) were significant predictors of increased long-term survival. CONCLUSION: Short-term survival of patients with initially unresectable ruptured HCC could be achieved in the presence of better Child-Pugh class, absence of shock, and with the use of aggressive initial treatment strategies. Among survivors of the emergency phase of tumor rupture, long-term survival was significantly increased with early modified LCSGJ stage and staged hepatectomy therapy.
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- 2020
19. Bronchial Artery Embolization for the Management of Frequent Hemoptysis Caused by Bronchiectasis
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Guang-Dong, Lu, Hai-Tao, Yan, Jin-Xing, Zhang, Sheng, Liu, Hai-Bin, Shi, and Qing-Quan, Zu
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Pulmonary and Respiratory Medicine ,Hemoptysis ,Treatment Outcome ,Recurrence ,Humans ,Bronchial Arteries ,Embolization, Therapeutic ,Retrospective Studies ,Bronchiectasis - Abstract
Background To retrospectively evaluate the effectiveness of bronchial artery embolization (BAE) compared with conservative therapy for the treatment of frequent hemoptysis caused by bronchiectasis. Methods From January 2015 to December 2019, consecutive patients who were admitted due to frequent (more than three times per year) bronchiectasis-related hemoptysis were retrospectively reviewed. Those who were treated with either BAE (n = 69) or conservative therapy (n = 47) were enrolled for analysis. The technical success, clinical success, and complications of the BAE procedure were evaluated. Long-term hemoptysis-free survival rates and clinical success were compared between patients in the BAE group and patients in the conservative group. A Cox proportional hazard regression model was used to identify the predictors of recurrent hemoptysis. Results The technical success rate was 100% for the BAE procedure, and clinical success was achieved in 92.8% (64 of 69) of cases. No major procedure-related complications occurred, and minor complications were observed in 16 cases (23.2%). The 1-, 2-, and 3-year hemoptysis-free survival rates were 88.3, 71.3, and 66.2%, respectively, for the BAE group and 31.9, 17.6, and 2.5%, respectively, for the conservative treatment group (P Conclusions BAE may provide an effective option for patients with frequent bronchiectasis-related hemoptysis, especially for those without cystic bronchiectasis.
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- 2022
20. Safety and Efficacy of Transarterial Chemoembolization and Immune Checkpoint Inhibition with Camrelizumab for Treatment of Unresectable Hepatocellular Carcinoma
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Jin-Xing Zhang, Pei Chen, Sheng Liu, Qing-Quan Zu, Hai-Bin Shi, and Chun-Gao Zhou
- Subjects
Journal of Hepatocellular Carcinoma - Abstract
Jin-Xing Zhang,1,* Pei Chen,2,* Sheng Liu,1 Qing-Quan Zu,1 Hai-Bin Shi,1 Chun-Gao Zhou1 1Department of Interventional Radiology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, Peopleâs Republic of China; 2Department of Basic Medicine, Jiangsu College of Nursing, Huaiâan, 223005, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Hai-Bin Shi; Chun-Gao Zhou, Department of Interventional Radiology, The First Affiliated Hospital with Nanjing Medical University, No. 300 Guangzhou Road, Nanjing, 210029, Peopleâs Republic of China, Tel +86-25-68306316 ; +86-13770598792, Fax +86-25-83724440, Email shihb@njmu.edu.cn; 1062874094@qq.comBackground: The clinical outcomes of hepatocellular carcinoma (HCC) patients who receive transarterial chemoembolization (TACE) and immunotherapy are not well characterized. The present study evaluates the safety and efficacy of TACE in combination with immune checkpoint inhibitor treatment for unresectable HCC.Methods: A retrospective analysis of 34 HCC patients who received TACE and treatment with the immune checkpoint inhibitor (ICI), Camrelizumab, between July 2019 and May 2021, was performed. This included 21 patients who developed progressive disease and eight who remained stable after several sessions of TACE, along with five patients who were initially diagnosed with advanced HCC. Adverse events (AEs), objective response rate (ORR) according to modified response evaluation criteria in solid tumors, progression-free survival (PFS), and overall survival (OS) were evaluated.Results: The median follow-up from ICI initiation was 10.6 months (range: 2.4â 25.0 months). Grade I/II and grade III/IV AEs from ICI treatment occurred in 20 (58.8%) and 2 patients (5.9%), respectively. Two to three months after ICI therapy, the ORR was 35.3% (12/34) and the median PFS and OS was 6.1 months (range: 1.1â 19.3 months) and 13.3 months (range: 2.4â 25.0 months), respectively.Conclusion: TACE in combination with ICI could be a promising treatment approach for unresectable HCC patients.Keywords: efficacy, hepatocellular carcinoma, immune checkpoint inhibition, safety, transarterial chemoembolization
- Published
- 2022
21. Outcomes between in-hospital stroke and community-onset stroke after thrombectomy: Propensity-score matching analysis
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Sheng Liu, Hai-Bin Shi, Lin-Bo Zhao, Kai Qiu, and Qing-Quan Zu
- Subjects
medicine.medical_specialty ,business.industry ,Endovascular Procedures ,Original Articles ,medicine.disease ,Hospitals ,Brain Ischemia ,Stroke ,Treatment Outcome ,Emergency medicine ,Propensity score matching ,medicine ,Humans ,cardiovascular diseases ,In hospital stroke ,business ,Propensity Score ,Community onset ,Ischemic Stroke ,Thrombectomy - Abstract
Background The benefit of endovascular thrombectomy for patients with in-hospital stroke remains unclear. Thus, the aim of this study was to compare the endovascular thrombectomy outcomes between in-hospital stroke and community-onset stroke among patients with acute ischemic stroke. Methods From January 2015 to July 2019, 362 consecutive patients with acute ischemic stroke with large vessel occlusion in the anterior circulation received endovascular thrombectomy in our centre. After propensity score matching with a ratio of 1:2 (in-hospital stroke:community-onset stroke), clinical characteristics and functional outcomes were compared between in-hospital stroke and community-onset stroke groups. Results Thirty-six patients with in-hospital stroke and 72 patients with community-onset stroke were enrolled. The number of patients with New York Heart Association classification III/IV (41.7% vs. 6.9%, p Conclusion Patients with in-hospital stroke had more disadvantageous comorbidities than those with community-onset stroke. Cardiac dysfunction seems to be associated with poor outcomes after thrombectomy. Nevertheless, endovascular thrombectomy still appears to be safe and effective for patients with in-hospital stroke.
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- 2021
22. Retrospective comparison of different percutaneous approaches to manage occluded primary uncovered self-expandable metal stents in patients with unresectable malignant hilar biliary obstruction
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Jin-Xing Zhang, Qing-Quan Zu, Hai-Bin Shi, Sheng Liu, Bin Wang, and Jin Liu
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Adult ,Male ,medicine.medical_specialty ,Percutaneous ,Self Expandable Metallic Stents ,03 medical and health sciences ,0302 clinical medicine ,Self-expandable metallic stent ,medicine ,Humans ,In patient ,Aged ,Retrospective Studies ,Aged, 80 and over ,Biliary tract neoplasm ,Cholestasis ,business.industry ,Gastroenterology ,Middle Aged ,Self Expandable Metal Stents ,surgical procedures, operative ,Bile Duct Neoplasms ,030220 oncology & carcinogenesis ,Drainage ,Equipment Failure ,Female ,030211 gastroenterology & hepatology ,Radiology ,business - Abstract
Purpose: To compare different percutaneous approaches to manage occluded primary uncovered self-expandable metal stents (SEMS) in patients with unresectable malignant hilar biliary obstruct...
- Published
- 2019
23. Prognostic value of elevated high-sensitivity cardiac troponin T levels in patients with acute ischemic stroke treated with endovascular thrombectomy
- Author
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Hai-Bin Shi, Chun-Gao Zhou, Qing-Quan Zu, Lei Jiang, Yue-Zhou Cao, Jin-Guo Xia, Qiang-Hui Liu, Wen-Tao Wu, Shan-Shan Lu, Zhen-Yu Jia, Sheng Liu, Xiao-Quan Xu, Lin-Bo Zhao, and Wei-Zhong Zhou
- Subjects
Male ,medicine.medical_specialty ,Cardiac troponin ,Brain Ischemia ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Troponin T ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,In patient ,Stroke ,Acute ischemic stroke ,Aged ,Retrospective Studies ,Thrombectomy ,Univariate analysis ,business.industry ,Endovascular Procedures ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Coronary heart disease ,Neurology ,030220 oncology & carcinogenesis ,Heart failure ,Cardiology ,Female ,Surgery ,Neurology (clinical) ,business ,Biomarkers ,030217 neurology & neurosurgery ,Large vessel occlusion - Abstract
Our objective was to assess the impact of hs-cTnT elevation on functional outcome and mortality in AIS patients with large vessel occlusion (LVO) in the anterior circulation 3 months after ET and explore factors affecting hs-cTnT elevation. A total of 143 consecutive AIS patients with large vessel occlusion (LVO) in the anterior circulation following ET in a single stroke center were enrolled between January 2015 and November 2017. Hs-cTnT was quantitated on admission. Demographic characteristics, clinical data, functional outcome and all-cause mortality were compared between patients with elevated hs-cTnT levels (14 ng/L) and those with normal hs-cTnT levels (≤14 ng/L). 58/143(40.6%) patients showed elevated hs-cTnT levels before ET. Factors independently associated with hs-cTnT elevation were admission NIHSS score (OR = 1.08, 95% CI 1.01-1.16, p = 0.032), coronary heart disease (OR = 4.89, 95% CI 1.82-13.11, p = 0.002) and congestive heart failure (OR = 4.10, 95% CI 1.07-15.68, p = 0.039). In the univariate analysis, patients with elevated hs-cTnT levels were at significantly higher risk of 3-month poor outcome (p = 0.029) and mortality (p 0.001) than those with normal hs-cTnT levels. After multivariable analysis, hs-cTnT elevation remained an independent predictor of 3-month mortality (OR = 4.49, 95% CI 1.68-11.98, p = 0.003). In this cohort of AIS patients with LVO in the anterior circulation undergoing ET, hs-cTnT elevation is an independent predictor of 3-month mortality. Admission NIHSS score, coronary heart disease and congestive heart failure are independently associated with elevated hs-cTnT levels.
- Published
- 2019
24. Efficacy and prognostic factors of transarterial embolization as initial treatment for spontaneously ruptured hepatocellular carcinoma: a single-center retrospective analysis in 57 patients
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Sheng Liu, Bin Wang, Chun-Gao Zhou, Hai-Bin Shi, Chun Zhou, and Qing-Quan Zu
- Subjects
Adult ,Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,medicine.medical_treatment ,Single Center ,Gastroenterology ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Embolization ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Rupture, Spontaneous ,business.industry ,Proportional hazards model ,Liver Neoplasms ,Hazard ratio ,Middle Aged ,Prognosis ,medicine.disease ,Embolization, Therapeutic ,Confidence interval ,Survival Rate ,Radiation therapy ,Treatment Outcome ,Liver ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Multivariate Analysis ,Lipiodol ,Female ,business ,medicine.drug - Abstract
To evaluate the efficacy and prognostic factors following transarterial embolization (TAE) as initial treatment for spontaneously ruptured hepatocellular carcinoma (HCC). From June 2012 to June 2016, a total of 57 spontaneously ruptured HCC patients who received TAE as initial therapy were retrospectively studied at our institution. Patients were followed until October 2016. Survival rates were estimated using the Kaplan–Meier method, and prognostic factors related to survival were evaluated using Cox regression analysis. Among them, 8 (14.0%) patients died within 30 days after initial TAE. The median overall survival time was 208 days. The 6-month, 1-year, and 2-year cumulative overall survival rates were 50.3%, 35.9%, and 14.7%, respectively. Multivariate analysis indicated that tumor size [HR (hazard ratio) 1.12; 95% CI (confidence interval) 1.02–1.23, P = 0.022] Child–Pugh class (HR 1.78; 95% CI 1.13–2.80; P = 0.013), absence of shock on admission (HR 0.40; 95% CI 0.17–0.95; P = 0.039), and embolization with lipiodol and gelatin sponge particles (HR 0.33; 95% CI 0.16–0.70; P = 0.004) were independent prognostic factors for survival. TAE appeared to be an effective initial treatment for spontaneously ruptured HCC. The results indicated that large tumors, poor Child–Pugh class, and shock on admission were associated with poor prognosis. For these patients, embolization with lipiodol and gelatin sponge particles could achieve better survival than PVA particles used.
- Published
- 2018
25. Ischemic Stroke Increased Gadolinium Deposition in the Brain and Aggravated Astrocyte Injury After Gadolinium-Based Contrast Agent Administration: Linear Versus Macrocyclic Agents
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Qing-Quan Zu, Fei-Yun Wu, Hai-Bin Shi, Run-Hao Jiang, Sheng Liu, Xin-Xin Huang, and Xiao-Quan Xu
- Subjects
Gadolinium DTPA ,Male ,medicine.medical_specialty ,Gadolinium ,chemistry.chemical_element ,Contrast Media ,030218 nuclear medicine & medical imaging ,Gadobutrol ,Brain Ischemia ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Organometallic Compounds ,Animals ,Radiology, Nuclear Medicine and imaging ,Viability assay ,Stroke ,Ischemic Stroke ,chemistry.chemical_classification ,Reactive oxygen species ,Cerebrum ,business.industry ,Brain ,medicine.disease ,Magnetic Resonance Imaging ,Rats ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Astrocytes ,Analysis of variance ,business ,medicine.drug ,Astrocyte - Abstract
BACKGROUND Gadolinium (Gd)-based contrast agents (GBCAs) have been widely used in MRI. However, several studies have reported Gd deposition in the brain, which has raised concerns about safety. PURPOSE To investigate the effects of ischemic stroke on Gd deposition in the brain after repeated administration of linear or macrocyclic GBCAs and to determine whether GBCAs aggravate astrocyte injury after stroke. STUDY TYPE Animal study. ANIMAL MODEL Twenty-seven male Sprague-Dawley rats were randomized to an exposure group (n = 24) and a healthy control group (n = 3). Half of the exposure group (n = 12) underwent transient middle cerebral artery occlusion (tMCAO) and half (n = 12) had a sham procedure. In each subgroup (tMCAO or sham), the rats had repeated gadopentetate (n = 6) or gadobutrol (n = 6) injections. Oxygen-glucose deprivation and reoxygenation (OGD/R) was used as an in vitro model of stroke. ASSESSMENT On day 3 and day 28 after the last injection (p.i.), the Gd concentration in the cerebrum was quantified by inductively coupled plasma mass spectrometry. Cell viability, reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were analyzed in vitro. STATISTICAL TESTS One-way analysis of variance and two-sample t-tests were performed. RESULTS The Gd concentration in the ipsilateral hemisphere homogenates of tMCAO group was significantly higher than that in the brain homogenates of the sham group on day 3 p.i. of either gadobutrol (0.065 ± 0.006 vs. 0.042 ± 0.007 μg/g, P
- Published
- 2020
26. Arterial embolization for hemoptysis in patients with chronic pulmonary tuberculosis and in patients with bronchiectasis
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Qing-Quan Zu, Chun-Gao Zhou, Jin-Xing Zhang, Guang-Dong Lu, Hai-Bin Shi, Sheng Liu, and Jin-Guo Xia
- Subjects
Adult ,Male ,Hemoptysis ,medicine.medical_specialty ,Tuberculosis ,Computed Tomography Angiography ,medicine.medical_treatment ,Bronchial Arteries ,Recurrence ,Pulmonary tuberculosis ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,In patient ,Embolization ,Tuberculosis, Pulmonary ,Aged ,Retrospective Studies ,Aged, 80 and over ,Bronchiectasis ,Radiological and Ultrasound Technology ,business.industry ,Arterial Embolization ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Embolization, Therapeutic ,Surgery ,Female ,business ,Chronic pulmonary tuberculosis - Abstract
BackgroundPrevious studies suggest that recurrence of hemoptysis after arterial embolization is associated with the underlying pulmonary disease.PurposeTo compare the baseline information and imagi...
- Published
- 2018
27. Inhibition of the cyclophilin A–CD147 interaction attenuates right ventricular injury and dysfunction after acute pulmonary embolism in rats
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Lin-Bo Zhao, Hai-Bin Shi, Zhen-Yu Jia, Qing-Quan Zu, Jin-Xing Zhang, and Guang-Dong Lu
- Subjects
Male ,0301 basic medicine ,Heart Ventricles ,Cardiomyopathy ,Blood Pressure ,Inflammation ,030204 cardiovascular system & hematology ,Pharmacology ,Biochemistry ,Rats, Sprague-Dawley ,03 medical and health sciences ,Cyclophilin A ,0302 clinical medicine ,stomatognathic system ,Western blot ,Troponin I ,polycyclic compounds ,medicine ,Animals ,Humans ,Molecular Biology ,Cyclophilin ,Metalloproteinase ,medicine.diagnostic_test ,business.industry ,Cell Biology ,medicine.disease ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Echocardiography ,Ventricle ,Basigin ,medicine.symptom ,Pulmonary Embolism ,business ,Protein Binding ,Signal Transduction - Abstract
Acute pulmonary embolism (APE)-induced inflammation contributes to cardiomyocyte injury and dysfunction in the right ventricle (RV) of the heart. The interactions of cyclophilin A with its ligand extracellular matrix metalloproteinase inducer (EMMPRIN or CD147) may be involved in this inflammatory process. To this end, here we induced APE by intravenous injections of microspheres in Sprague-Dawley rats. We found that after the APE, cyclophilin A and CD147 levels increased synchronously in RV tissue following APE and peaked at 24 h. The cyclophilin A inhibitor cyclosporine A attenuated the APE-induced cyclophilin A levels, and a monoclonal antibody of CD147 (anti-CD147) abrogated the elevation of CD147 in the RV but not the increase of cyclophilin A. Importantly, treatment with cyclosporine A, anti-CD147, or both attenuated APE-induced increases in RV systolic pressure, plasma cardiac troponin-I (cTnI) concentrations, the RV/left ventricle diameter ratio, and the Tei index, measured by echocardiography 24 h after APE induction. These beneficial effects were associated with reduced RV neutrophil infiltration and prevention of matrix metalloproteinase 9 (MMP-9) and MMP-2 activation. These findings suggested that inhibiting the cyclophilin A–CD147 interaction attenuates APE-associated RV cardiomyocyte injury and dysfunction by suppressing inflammation. We further proposed that cyclophilin A and CD147 might participate in APE-induced pathological processes by partly activating the ERK1/2 kinase–nuclear factor-κB pathway. We conclude that the cyclophilin A–CD147 interaction may represent a potential therapeutic target for managing APE.
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- 2018
28. Risk factors contributing to early and late recurrence of haemoptysis after bronchial artery embolisation
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Jin-Xing Zhang, Guang-Dong Lu, Hai-Bin Shi, Sheng Liu, Chun-Gao Zhou, Wei Ye, Jin-Guo Xia, and Qing-Quan Zu
- Subjects
Male ,Pulmonary and Respiratory Medicine ,Hemoptysis ,medicine.medical_specialty ,Time Factors ,Tuberculosis ,medicine.medical_treatment ,Bronchial Arteries ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Recurrence ,Risk Factors ,medicine.artery ,Late Recurrence ,medicine ,Humans ,030212 general & internal medicine ,Embolization ,Retrospective Studies ,Lung ,Proportional hazards model ,business.industry ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Collateral circulation ,Embolization, Therapeutic ,Surgery ,Treatment Outcome ,Infectious Diseases ,medicine.anatomical_structure ,030228 respiratory system ,Regression Analysis ,Female ,Bronchial artery ,business - Abstract
Objective To identify the risk factors for early and late relapse of haemoptysis after bronchial artery embolisation (BAE). Design We performed a retrospective study of 255 patients with haemoptysis who underwent BAE from January 2009 to June 2016 at the First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Using a Cox regression model, risk factors contributing to early (within the first month) and late (>1 month) recurrence were analysed. We censored recurrence-free patients at 1 month and patients without rebleeding after 1 month. Results The cumulative recurrence rate at 1, 6, 12, 24 and 48 months was respectively 14.9%, 16.9%, 20.6%, 31.0% and 39.9%, with a median follow-up of 574 days. Early relapse occurred in 38 of 255 patients, while late recurrence was found in 45 of the remaining 217 patients. The risk factors related to early recurrence were lung destruction on computed tomography of the chest and involvement of non-bronchial systemic arteries. The risk factors associated with late recurrence were tuberculosis sequelae, the presence of shunts and the use of gelfoam as an embolisation material. Conclusions The variables related to incomplete embolisation were the risk factors for early rebleeding. The variables associated with same-vessel recanalisation and formation of a new collateral circulation influenced late recurrence.
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- 2018
29. Differences in efficacy of uncovered self-expandable metal stent in relation to placement in the management of malignant distal biliary obstruction
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Qing-Quan Zu, Jin-Guo Xia, Hai-Bin Shi, Jin-Xing Zhang, Sheng Liu, and Chun-Gao Zhou
- Subjects
Male ,medicine.medical_specialty ,Percutaneous ,metal stent ,medicine.medical_treatment ,Self Expandable Metallic Stents ,Stent patency ,Group B ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Sphincter of Oddi ,medicine ,Humans ,In patient ,lcsh:RC799-869 ,Aged ,Retrospective Studies ,Distal biliary obstruction ,Cholangiopancreatography, Endoscopic Retrograde ,Cholestasis ,Self expandable ,business.industry ,sphincter of Oddi ,Gastroenterology ,Stent ,Patient survival ,Middle Aged ,equipment and supplies ,Survival Analysis ,Surgery ,Bile Duct Neoplasms ,outcome ,Original Article ,Female ,030211 gastroenterology & hepatology ,lcsh:Diseases of the digestive system. Gastroenterology ,business - Abstract
Background/Aims: Metal stent insertion is a common palliative treatment for distal malignant biliary obstruction (MBO) but whether placement across the sphincter of Oddi (SO) causes more complications or shorter survival is in question. The aim of this study was to compare the clinical outcomes of percutaneous uncovered self-expandable metal stent placement above and across the SO in patients with distal MBO. Patients and Methods: We retrospectively studied 59 patients who underwent uncovered metal stent placement for distal MBO between January 2012 and March 2016. Stents were placed above the SO for 22 subjects (group A) and across the SO for 37 subjects (group B). Early cholangitis, stent occlusion, and overall survival time were compared between the two groups. Results: Clinical success was 90.9 and 86.5% for groups A and B, respectively. Early cholangitis occurred in 2 patients (9.1%) in group A and in 6 patients (16.2%) in group B (P = 0.645). Median stent patency and median survival time was 105 and 140 days for group A, 120 and 160 days for group B, respectively. The stent occlusion (31.8% in group A and 21.6% in group B, P = 0.454) and the cumulative stent patency times (P = 0.886) did not differ between the two groups. There was no significant difference in cumulative patient survival between the two groups (P = 0.810). Conclusion: Uncovered metal stent placement across the SO did not significantly affect early cholangitis, stent patency, or patient survival.
- Published
- 2018
30. Recovery of oculomotor nerve palsy after endovascular treatment of ruptured posterior communicating artery aneurysm
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Qing-Quan Zu, Chun-Gao Zhou, Xing-Long Liu, Hai-Bin Shi, Jin-Guo Xia, Lin-Bo Zhao, Bin Wang, and Sheng Liu
- Subjects
Adult ,Male ,medicine.medical_specialty ,Subarachnoid hemorrhage ,medicine.medical_treatment ,Aneurysm, Ruptured ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Aneurysm ,medicine.artery ,Oculomotor Nerve Diseases ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Embolization ,Posterior communicating artery ,Oculomotor nerve palsy ,Aged ,Neuroradiology ,Aged, 80 and over ,business.industry ,Intracranial Aneurysm ,Retrospective cohort study ,Recovery of Function ,Middle Aged ,medicine.disease ,Embolization, Therapeutic ,Cerebral Angiography ,Treatment Outcome ,cardiovascular system ,Female ,Neurology (clinical) ,Radiology ,Neurosurgery ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
Oculomotor nerve palsy (ONP) may result from Posterior communicating artery (Pcom) aneurysms. Endovascular treatment of ruptured Pcom aneurysms generally is a safe procedure, but the effect of this therapy on ONP is incompletely elucidated. This retrospective study evaluates outcomes of ONP after endovascular treatment for ruptured Pcom aneurysm and with the intention to clarify predictors of recovery. From May 2010 to October 2015, 210 patients with Pcom aneurysms underwent endovascular treatment at our institution. Among them, 34 patients with ruptured aneurysms and either complete or incomplete ONP were identified. The outcomes and predictors of ONP recovery were analyzed. At the last available clinical follow-up, ONP resolution was complete in 21 (61.8%) patients and incomplete in 8 (23.5%) patients. The mean resolution time after embolization was 24.5 days. Five patients showed no signs of ONP recovery. In no case was an initial incomplete ONP observed to worsen. There was a statistically insignificant trend toward complete recovery among patients with initial incomplete ONP (OR = 4.17; 95% CI, 0.75–23.18; P = 0.103). Endovascular treatment appears to be an effective treatment modality for ruptured Pcom aneurysm and related ONP. The initial incomplete ONP might encourage complete ONP recovery after endovascular treatment.
- Published
- 2017
31. A Canine Model of Hemorrhagic Transformation Using Recombinant Tissue Plasminogen Activator Administration After Acute Ischemic Stroke
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Sheng Liu, Bin Wang, Xiao-Quan Xu, Ye Ding, Hai-Bin Shi, Run-Hao Jiang, Qing-Quan Zu, and Jun Wang
- Subjects
0301 basic medicine ,Pharmacology ,Blood–brain barrier ,Immunofluorescence ,lcsh:RC346-429 ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,medicine.artery ,ischemic stroke ,medicine ,canines ,Recombinant tissue plasminogen activator ,Acute ischemic stroke ,Stroke ,lcsh:Neurology. Diseases of the nervous system ,Original Research ,medicine.diagnostic_test ,business.industry ,blood-brain barrier ,medicine.disease ,hemorrhagic transformation ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Middle cerebral artery ,Ischemic stroke ,Neurology (clinical) ,MMP-9 ,business ,030217 neurology & neurosurgery - Abstract
Early reperfusion of occluded arteries via recombinant tissue plasminogen activator (rtPA) administration is considered to be an effective strategy for the treatment of acute ischemic stroke. However, delayed administration of rtPA may cause severe hemorrhagic transformation (HT) and undesirable neurological outcomes. The current study aims to establish a canine HT model using rtPA administration and to investigate the potential mechanisms underlying HT. Following anesthesia, two autologous clots were injected into the middle cerebral artery (MCA) to induce ischemic stroke. To induce reperfusion, rtPA (2 mg/kg) was administrated intravenously 4.5 h after the establishment of stroke. The occurrence of HT was determined by computed tomography (CT) and by pathological assessment. Transmission electron microscopy was utilized to assess blood-brain barrier (BBB) damage. The expression of matrix metalloprotein 9 (MMP-9) was analyzed by enzyme linked immunosorbent assay (ELISA), immunofluorescence (IF), and western blot. Administration of rtPA 4.5 h after stroke induced reperfusion in 73.9% of the canines, caused evident HT, and did not improve neurological outcomes compared to canines that did not receive rtPA. There was a significant increase in expression of MMP-9 after rtPA administration, accompanied by BBB disruption. We have established a canine HT model that closely mimics human HT by using rtPA administration after the induction of middle cerebral artery occlusion (MCAO) with autologous clots. Our data suggest that a potential mechanism underlying rtPA-caused HT may be related to BBB dysfunction induced by an increase in MMP-9 expression.
- Published
- 2019
32. High b value DWI in evaluation of the hyperacute cerebral ischemia at 3T: A comparative study in an embolic canine stroke model
- Author
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Xiao-Quan Xu, Sheng Liu, Qiguang Cheng, Bin Wang, Hai-Bin Shi, Gaojun Teng, Shan-Shan Lu, Xing-Long Liu, Jing Yu, and Qing-Quan Zu
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Ischemia ,high b value ,cerebral ischemia ,030218 nuclear medicine & medical imaging ,onset time ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Internal medicine ,diffusion weighted imaging ,Medicine ,Effective diffusion coefficient ,Temporal change ,cardiovascular diseases ,Stroke ,relative signal intensity ,business.industry ,Area under the curve ,General Medicine ,Articles ,medicine.disease ,High B-Value ,Ischemic stroke ,Cardiology ,business ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
Previous studies have indicated that the temporal change of relative diffusion weighted imaging (rDWI) signal intensity may help to determine the onset time of a stroke. Furthermore, several studies have indicated that high b value DWI offered improved detection rates for hyper-acute ischemic lesions compared with standard b value DWI. However, the temporal changes of the rDWI on high b value DWI remain unclear. Therefore, based on our embolic canine stroke model, we evaluated the temporal evolution of rDWI on high b value DWI, and further compared its diagnostic value in predicting the onset time of ischemic stroke with rDWI on standard b value DWI. Twelve canine MCAO models were established, and DWI was performed at 1, 2, 3, 4, 5 and 6 h after MCAO, with 3 b values of 1,000, 2,000 and 3,000. High b value DWI detected all ischemic lesions after 1 h, while standard b value did not detect the ischemic lesions in one dog at 1 h. With all three of the tested b values, rDWIs increased continuously within 6 h, while relative apparent diffusion coefficient (rADC) values rapidly decreased in 1 h, then became relatively stable. The area under the curve values for rDWI with b value of 1,000, 2,000 and 3,000, in predicting ischemic lesions within 3 h were 0.897, 0.929 and 0.938, while for rADC were 0.645, 0.583 and 0.599, respectively. Therefore, the results indicated that the rDWI was helpful in aging hyper-acute ischemic stroke, while rADC appeared not to be. High b value DWI had a higher detection rate for ischemic lesions and better predictive efficacy in determining the onset time of hyper-acute stroke.
- Published
- 2016
33. Transarterial embolization in the management of intractable epistaxis: the angiographic findings and results based on etiologies
- Author
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Hai-Bin Shi, Jin-Guo Xia, Lin-Bo Zhao, Bin Wang, Qing-Quan Zu, Sheng Liu, Chun-Gao Zhou, and Xing-Long Liu
- Subjects
Adult ,Male ,Interventional therapy ,medicine.medical_specialty ,Adolescent ,030218 nuclear medicine & medical imaging ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Negative angiography ,Transarterial embolization ,Humans ,Medicine ,Effective treatment ,Child ,030223 otorhinolaryngology ,Aged ,Retrospective Studies ,medicine.diagnostic_test ,Benign disease ,business.industry ,Angiography ,General Medicine ,Middle Aged ,Embolization, Therapeutic ,Surgery ,Nasal bleeding ,Epistaxis ,Otorhinolaryngology ,Etiology ,Female ,Radiology ,business - Abstract
Transarterial embolization (TAE) appears to be a safe and effective treatment for patients with intractable epistaxis, despite different etiologies or angiography findings. Idiopathic epistaxis is prone to present with negative angiographic findings.To retrospectively evaluate the safety and effectiveness of TAE for intractable epistaxis, and focus on the factors of etiology and angiographic findings.From March 2008 to December 2014, the data of 43 patients with intractable bleeding undergoing TAE were reviewed. The outcomes of interventional therapy were assessed according to different etiology (malignant or benign disease) and angiographic finding (positive or negative angiogram).Positive angiographic findings were found in 11 of 12 cases with malignant diseases and 22 of 31 cases with benign diseases, respectively (p = 0.237). Among the 10 cases with negative angiographic findings, the negative angiography rate of idiopathic epistaxis was higher than that of epistaxis with definite etiology (p = 0.003). Bleeding was controlled successfully in all of the 43 patients after embolization. During the mean follow-up period of 24.0 ± 16.7 months, five patients relapsed. No significant difference was found in recurrence rates between malignant and benign diseases or between positive and negative angiography (p = 0.241, p = 0.704, respectively).
- Published
- 2016
34. Embolisation for life-threatening haemoptysis complicated by systemic artery–pulmonary circulation shunts
- Author
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Guang-Dong Lu, Hai-Bin Shi, Sheng Liu, Qing-Quan Zu, Bin Wang, Xing‑Long Liu, Chun-Gao Zhou, and Jin-Guo Xia
- Subjects
Adult ,Lung Diseases ,Male ,Pulmonary and Respiratory Medicine ,Hemoptysis ,Pulmonary Circulation ,Disease free survival ,medicine.medical_specialty ,Time Factors ,Tertiary referral centre ,medicine.medical_treatment ,education ,Treatment outcome ,Bronchial Arteries ,Pulmonary Artery ,Disease-Free Survival ,030218 nuclear medicine & medical imaging ,Tertiary Care Centers ,03 medical and health sciences ,0302 clinical medicine ,Recurrence ,medicine.artery ,medicine ,Humans ,Embolization ,Referral and Consultation ,Aged ,Retrospective Studies ,business.industry ,Retrospective cohort study ,Middle Aged ,equipment and supplies ,Embolization, Therapeutic ,Surgery ,Treatment Outcome ,Infectious Diseases ,medicine.anatomical_structure ,Pulmonary Veins ,Polyvinyl Alcohol ,030220 oncology & carcinogenesis ,Female ,Bronchial artery ,business ,Shunt (electrical) ,Artery - Abstract
Setting Tertiary referral centre. Objective To retrospectively observe the characteristics of bronchial angiograms in the setting of systemic artery-pulmonary circulation shunts (SPS), and to evaluate the safety and effectiveness of bronchial artery embolisation (BAE) for these patients with life-threatening haemoptysis. Design The records of life-threatening haemoptysis patients with SPS who presented to a tertiary referral centre from January 2009 to March 2014 were reviewed. Results SPS consisted of bronchial artery-pulmonary artery shunt (AAS) in 30 cases, bronchial artery-pulmonary vein shunt (AVS) in 4 cases, non-bronchial systemic artery-pulmonary circulation shunt (n-BPS) in 7 cases and more than one type of SPS in 4 cases (AAS and AVS in 3 cases, three types of SPS in 1 case). BAE using polyvinyl alcohol (PVA) was successful in 97.8% (44/45) of the patients. Cumulative rates of freedom from recurrence at 1 month, 1 year and 2 years were respectively 97.8%, 93.2% and 85.4%. No major procedure-related complications occurred. No significant differences were found in recurrence rates or cumulative haemoptysis control rates among patients with different types of SPS complications (P = 0.55 and 0.46, respectively). Conclusion BAE with PVA was safe and effective for life-threatening haemoptysis complicated by SPS.
- Published
- 2016
35. Percutaneous transpapillary biliary stent placement for distal malignant biliary obstruction: Outcomes and survival analysis
- Author
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Wei Ye, Qing-Quan Zu, Bin Wang, Sheng Liu, Jin-Xing Zhang, and Hai-Bin Shi
- Subjects
Male ,medicine.medical_specialty ,Percutaneous ,medicine.medical_treatment ,Self Expandable Metallic Stents ,Metastatic carcinoma ,Self-expandable metallic stent ,Risk Factors ,medicine ,Humans ,Survival rate ,Survival analysis ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Cholestasis ,business.industry ,Gastroenterology ,Stent ,Middle Aged ,medicine.disease ,Surgery ,Survival Rate ,Biliary Tract Surgical Procedures ,Treatment Outcome ,Bile Duct Neoplasms ,Pancreatitis ,Original Article ,Female ,business ,Complication - Abstract
BACKGROUND/AIMS: For distal malignant biliary obstruction (MBO), a percutaneous metal stent is usually inserted by the transpapillary method. However, stent-related complications and recurrent biliary obstruction following transpapillary stent placement are concerns, and survival analysis of patients with distal MBO has rarely been done. MATERIALS AND METHODS: From January 2012 to March 2016, 104 patients underwent transpapillary uncovered metal stent placement for distal MBO at our institution. Clinical success, complications, recurrent biliary obstruction rates, and predictors of survival were analyzed. RESULTS: Of the total 104 patients, clinical success after stent insertion was achieved in 93 patients (90.3%). Major complications were observed in 24 patients (23.1%), which were as follows: cholangitis in 19 patients; pancreatitis in four patients; and biloma in one patient. Recurrent biliary occlusion was observed in 28 patients (26.9%). The median overall survival period was 162 days. The 3-, 6-, and 12-month overall survival rates after stent insertion were 64.4%, 41.3%, and 10.6%, respectively. Results of multivariate analysis indicated that metastatic carcinoma compared with ampullary carcinoma (HR=3.82; 95% CI, 1.30–11.24; p=0.015) and longer biliary stricture (HR=1.04; 95% CI, 1.02–1.06; p
- Published
- 2018
36. Predictors of Recurrent Biliary Obstruction Following Percutaneous Uncovered Metal Stent Insertion in Patients with Distal Malignant Biliary Obstruction: An Analysis Using a Competing Risk Model
- Author
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Qing-Quan Zu, Bin Wang, Hai-Bin Shi, Jin-Xing Zhang, and Sheng Liu
- Subjects
Male ,medicine.medical_specialty ,Percutaneous ,Multivariate analysis ,030218 nuclear medicine & medical imaging ,Metastatic carcinoma ,03 medical and health sciences ,0302 clinical medicine ,Self-expandable metallic stent ,Recurrence ,Risk Factors ,Sphincter of Oddi ,Medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Biliary tract neoplasm ,Cholestasis ,business.industry ,Hazard ratio ,Survival Analysis ,Confidence interval ,Surgery ,Biliary Tract Neoplasms ,Metals ,Female ,Stents ,Cardiology and Cardiovascular Medicine ,business - Abstract
To evaluate predictive factors of recurrent biliary obstruction (RBO) following percutaneous uncovered metal stent placement for unresectable distal malignant biliary obstruction (MBO) by using a competing risk model. Between March 2012 and March 2016, 119 patients underwent percutaneous uncovered metal stent placement with distal MBO at our institution. Univariate and multivariate analyses were conducted to identify the prognostic factors for RBO using a competing risk model. The median overall survival period was 170 days (range 19–775 days). Recurrent biliary obstruction before death was observed in 34 patients (28.6%). The 3-, 6-, and 12-month cumulative incidences of RBO were 14.3%, 21.0%, and 27.7%, respectively. A multivariate analysis indicated that ampullary carcinoma compared with metastatic carcinoma (hazard ratio [HR] = 4.86; 95% confidence interval [CI], 1.74–13.54; P = 0.003) and a stent insertion above the sphincter of Oddi (HR 2.49; 95% CI, 1.11–5.62; P = 0.028) were the independent risk factors for RBO when we considered death to be a competing risk. Ampullary carcinoma and stent insertion above the sphincter of Oddi were risk factors for RBO in these patients who received percutaneous transhepatic stent placement.
- Published
- 2018
37. Effects of Diabetes Mellitus and Admission Glucose in Patients Receiving Mechanical Thrombectomy: A Systematic Review and Meta-analysis
- Author
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Hai-Bin Shi, Qing-Quan Zu, Zi-Qi Ren, Guang-Dong Lu, and Jin-Xing Zhang
- Subjects
medicine.medical_specialty ,Neurology ,Mechanical Thrombolysis ,Comorbidity ,030204 cardiovascular system & hematology ,Critical Care and Intensive Care Medicine ,Brain Ischemia ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,Outcome Assessment, Health Care ,medicine ,Clinical endpoint ,Diabetes Mellitus ,Humans ,In patient ,business.industry ,Odds ratio ,medicine.disease ,Confidence interval ,Mechanical thrombectomy ,Stroke ,Meta-analysis ,Hyperglycemia ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
We performed a meta-analysis to evaluate the outcomes of acute ischemic stroke (AIS) in patients treated with mechanical thrombectomy (MT), according to diabetes mellitus and admission glucose level (AGL). We systematically reviewed previous studies in PubMed that reported outcomes of MT in AIS patients and their relationships with diabetes mellitus or AGL. We used functional independence (modified Rankin score ≤ 2 at 3 months) as the primary end point. Data from 12,653 patients in 47 articles that evaluated the effect of diabetes mellitus or AGL on outcomes after MT were included. Compared with patients without a history of diabetes mellitus, patients with a diabetes mellitus history had significantly lower odds of functional independence in both the unadjusted meta-analysis (odds ratio [OR] 0.64; 95% confidence interval [CI] 0.54–0.75) and the multivariable analysis (OR 0.48; 95% CI 0.33–0.71). Similarly, higher AGL was associated with an unfavorable functional outcome in the unadjusted meta-analysis (pooled effect size − 0.38; 95% CI − 0.45 to − 0.31), and the adjusted OR (95% CI) per 1 mmol/L increase in AGL was 0.87 (0.83–0.92) for functional independence according to the combined multivariable results. Recanalization rate and symptomatic intracranial hemorrhage were neither related to AGL nor different in patients with or without diabetes mellitus. The present study confirms that a history of diabetes mellitus and high AGL are associated with unfavorable functional outcomes at 3 months after MT in AIS patients. However, the causal relationship between hyperglycemia and poor prognosis remains undetermined, and further investigations are required to ascertain whether AIS patients receiving MT could benefit from intensive glucose control.
- Published
- 2018
38. The CD40/CD40L system regulates rat cerebral microvasculature after focal ischemia/reperfusion via the mTOR/S6K signaling pathway
- Author
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Run-Hao Jiang, Shan-Shan Lu, Hai-Bin Shi, Sheng Liu, Chen-Jiang Wu, Xiao-Quan Xu, Lin-Bo Zhao, and Qing-Quan Zu
- Subjects
Blood Platelets ,Male ,CD40 Ligand ,Focal ischemia ,chemical and pharmacologic phenomena ,P70-S6 Kinase 1 ,Brain Edema ,Capillary Permeability ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,immune system diseases ,Medicine ,Animals ,CD40 Antigens ,PI3K/AKT/mTOR pathway ,CD40 ,biology ,Microvascular thrombosis ,business.industry ,Ribosomal Protein S6 Kinases ,TOR Serine-Threonine Kinases ,Brain ,Endothelial Cells ,hemic and immune systems ,Infarction, Middle Cerebral Artery ,General Medicine ,stomatognathic diseases ,Disease Models, Animal ,Neurology ,030220 oncology & carcinogenesis ,Cd40 cd40l ,Reperfusion Injury ,Microvessels ,biology.protein ,Cancer research ,Neurology (clinical) ,Signal transduction ,business ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
The role of CD40/CD40 ligand (CD40L) in microvascular thrombosis is now widely accepted. However, the exact mechanisms linking the CD40/CD40L system and the soluble form of CD40L (sCD40L) with microvascular thrombosis are currently a topic of intensive research. The objective of this study was to assess the potential mechanisms in CD40/CD40L system-regulated microvascular thrombosis after focal ischemia/reperfusion (I/R).Rats were subjected to 60-min transient middle cerebral artery occlusion (MCAO). The experiments were divided into three groups: sham operation, MCAO, and MCAO + CD40 antagonist. Dynamic changes of serum-free sCD40L levels for 0, 1, 3, 5, 6, and 12 h by ELISA detecting kit after focal I/R were observed, and the CD40 expression levels in both platelet surface and vascular endothelial cell surface were measured by flow cytometry and immunofluorescence, respectively. Cerebral infarct volume was analyzed 12 h after reperfusion. mTOR/S6K signaling was determined by Western blot.A comparison of thrombus formation between MCAO and CD40 antagonist treatment rats revealed a role for CD40 and/or CD40L in the inflammation-enhanced thrombosis responses in both of the platelet and vascular endothelial cell. MCAO rats yielded an acceleration of thrombus formation that was accompanied by increased CD40 levels in serum. The brain infarction was significantly decreased in CD40 antagonist treatment group compared to MCAO model group. The mTOR/S6K signaling was activated in MACO model than that of CD40 antagonist treatment group.Our findings indicate that CD40/CD40L system contributes to microvascular thrombosis and brain infarction induced by MCAO and reperfusion. The mTOR/S6K signaling pathway is involved in the regulation of cerebral microvasculature after focal I/R by CD40/CD40L.AKT: protein kinase B; CD40L: CD40 ligand; CSF: cerebrospinal fluid; FITC: fluorescein isothiocyanate; I/R: ischemia/reperfusion; MCAO: middle cerebral artery occlusion; mTOR: mechanistic target of rapamycin; PE: P-phycoerythrin; sCD40L: soluble form of CD40L; TNF-a: tumor necrosis factor-alpha; WT: wild type.
- Published
- 2018
39. Hypoxic conditioned medium derived from bone marrow mesenchymal stromal cells protects against ischemic stroke in rats
- Author
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Hai-Bin Shi, Qing-Quan Zu, Lin-Bo Zhao, Xiao-Quan Xu, Run-Hao Jiang, Chen-Jiang Wu, Shan-Shan Lu, Jun Wang, and Sheng Liu
- Subjects
0301 basic medicine ,Male ,Physiology ,Angiogenesis ,medicine.medical_treatment ,Clinical Biochemistry ,Neovascularization, Physiologic ,Apoptosis ,Bone Marrow Cells ,Pharmacology ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Animals ,Phosphorylation ,Stroke ,Cells, Cultured ,business.industry ,Mesenchymal stem cell ,Brain ,Infarction, Middle Cerebral Artery ,Mesenchymal Stem Cells ,Cell Biology ,Recovery of Function ,medicine.disease ,Cell Hypoxia ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Cytokine ,Neuroprotective Agents ,030220 oncology & carcinogenesis ,Culture Media, Conditioned ,Cytokines ,Bone marrow ,Stem cell ,Phosphatidylinositol 3-Kinase ,business ,Proto-Oncogene Proteins c-akt - Abstract
In recent years, studies have shown that the secretome of bone marrow mesenchymal stromal cells (BMSCs) contains many growth factors, cytokines, and antioxidants, which may provide novel approaches to treat ischemic diseases. Furthermore, the secretome may be modulated by hypoxic preconditioning. We hypothesized that conditioned medium (CM) derived from BMSCs plays a crucial role in reducing tissue damage and improving neurological recovery after ischemic stroke and that hypoxic preconditioning of BMSCs robustly improves these activities. Rats were subjected to ischemic stroke by middle cerebral artery occlusion and then intravenously administered hypoxic CM, normoxic CM, or Dulbecco modified Eagle medium (DMEM, control). Cytokine antibody arrays and label-free quantitative proteomics analysis were used to compare the differences between hypoxic CM and normoxic CM. Injection of normoxic CM significantly reduced the infarct area and improved neurological recovery after stroke compared with administering DMEM. These outcomes may be associated with the attenuation of apoptosis and promotion of angiogenesis. Hypoxic preconditioning significantly enhanced these therapeutic effects. Fourteen proteins were significantly increased in hypoxic CM compared with normoxic CM as measured by cytokine arrays. The label-free quantitative proteomics analysis revealed 163 proteins that were differentially expressed between the two groups, including 107 upregulated proteins and 56 downregulated proteins. Collectively, our results demonstrate that hypoxic CM protected brain tissue from ischemic injury and promoted functional recovery after stroke in rats and that hypoxic CM may be the basis of a potential therapy for stroke patients.
- Published
- 2018
40. The Safety and Efficacy of Oxycodone Versus Fentanyl in Percutaneous Microwave Ablation of a Liver Tumour Abutting the Capsule
- Author
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Zheng-Qiang Yang, Hai-Bin Shi, Zhen-Yu Jia, Qing-Quan Zu, Qi-Feng Chen, Sheng Liu, Yu Chen, and Wen-Tao Wu
- Subjects
Ablation Techniques ,Male ,030218 nuclear medicine & medical imaging ,Fentanyl ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,General anaesthesia ,Prospective Studies ,Microwaves ,Pain, Postoperative ,business.industry ,Microwave ablation ,Liver Neoplasms ,Body movement ,Middle Aged ,Dezocine ,Analgesics, Opioid ,Treatment Outcome ,Opioid ,Liver ,Anesthesia ,Female ,Cardiology and Cardiovascular Medicine ,Propofol ,business ,Oxycodone ,medicine.drug - Abstract
The present study compared the safety and efficacy of oxycodone with those of fentanyl under non-intubated general anaesthesia in percutaneous microwave ablation (MWA) of a liver tumour abutting the capsule. Thirty-eight patients underwent MWA of liver cancers abutting the capsule. Patients received 0.1 mg/kg oxycodone (O group) or 1 μg/kg fentanyl (F group) prior to the start of ablation. Both groups received continuous infusions of propofol for non-intubated general anaesthesia during ablation. The primary outcomes were the pain scores (11-point numeric rating scale, NRS) within 24 h after MWA. Vital signs, body movement during ablation, and opioid side effects after ablation were recorded. The need for additional analgesics was recorded 24 h after MWA. The pain NRS scores were lower in the O group than in the F group at 0.5 (P = 0.035), 3 (P = 0.002), and 6 h (P = 0.001) after MWA, and fewer patients required additional analgesics in the O group (6 of 20 vs. 13 of 18, P = 0.022) within 24 h. The average 24-h dose of dezocine was 5.5 ± 4.1 mg in the F group and 2.1 ± 3.3 mg in the O group (P = 0.008). A significant reduction in the respiratory rate (P = 0.020) and more body movements were observed in the F group (P = 0.027) during ablation with non-intubated general anaesthesia. No differences in post-operative nausea and vomiting (PONV) were observed between the two groups, but dizziness occurred significantly more often in the O group (P = 0.033). No significant differences in other vital signs were observed before, during, and after the procedure. Oxycodone provides better analgesia and reduces post-operative opioid consumption without significant respiratory or hemodynamic instability.
- Published
- 2018
41. Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural‑like cells
- Author
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Sheng Liu, Xing‑Long Liu, Qing‑Quan Zu, Hai‑Bin Shi, Shan‑Shan Lu, Bin Wang, and Xiao‑Quan Xu
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Cellular differentiation ,Biochemistry ,Ferric Compounds ,Cell therapy ,03 medical and health sciences ,Paracrine signalling ,0302 clinical medicine ,Dogs ,Neurotrophic factors ,Genetics ,Animals ,Viability assay ,Magnetite Nanoparticles ,Molecular Biology ,Brain-derived neurotrophic factor ,Chemistry ,Brain-Derived Neurotrophic Factor ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,Transfection ,Cell biology ,030104 developmental biology ,Phenotype ,Oncology ,030220 oncology & carcinogenesis ,Molecular Medicine ,Female ,Biomarkers - Abstract
The use of mesenchymal stem cells (MSCs) has been reported to improve outcomes in various types of nervous system diseases, primarily based on their neural regenerative differentiation ability and paracrine effect on different neuroprotective cytokines. Genetically modified MSCs may enhance the paracrine effect and may further improve the cell‑based therapeutic outcome of nervous system diseases. Magnetic resonance imaging has been used to monitor distribution and migration of cells labeled with superparamagnetic iron oxide (SPIO) nanoparticles. However, few studies have described the neural differentiation ability of genetically modified and SPIO‑labeled MSCs, which is the foundation for cell tracking and cell therapy in vivo. In this study, canine bone marrow‑derived MSCs (BMSCs) were initially labeled with SPIO, by culturing with 20 µg/ml SPIO for 24 h, and transfected with the brain‑derived neurotrophic factor (BDNF) gene using lentivirus transfection at different multiplicities of infection (MOI) values. The optimized MOI value was demonstrated by cellular viability and enhanced green fluorescent protein (eGFP) rate. Subsequently, the BMSCs were induced to differentiate into neuron‑like cells by chemical induction. The results demonstrated that BDNF‑overexpressing BMSCs labeled with SPIO can be induced into neuron‑like cells with high efficiency and minimal effects on cell viability. Additionally, following neural differentiation, the cells transfected with BDNF and labeled with SPIO expressed significantly higher levels of BDNF and neural markers. The overexpression of BDNF may contribute to neural differentiation of BDNFs, and may have potential benefits for further BMSC‑based therapy in vivo.
- Published
- 2017
42. Comparative study of the relative signal intensity on DWI, FLAIR, and T2 images in identifying the onset time of stroke in an embolic canine model
- Author
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Qing-Quan Zu, Hai-Bin Shi, Sheng Liu, Shan-Shan Lu, Xiao-Quan Xu, Jing Yu, Qiguang Cheng, and Ye Sheng
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Time Factors ,Neurology ,Dermatology ,Fluid-attenuated inversion recovery ,Dogs ,Image Interpretation, Computer-Assisted ,medicine ,Animals ,Stroke ,Neuroradiology ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Angiography, Digital Subtraction ,Retrospective cohort study ,Magnetic resonance imaging ,General Medicine ,medicine.disease ,Magnetic Resonance Imaging ,Disease Models, Animal ,Psychiatry and Mental health ,Intracranial Embolism ,ROC Curve ,Sample size determination ,Female ,Neurology (clinical) ,Nuclear medicine ,business - Abstract
In acute stroke magnetic resonance imaging, many attempts have been made to identify the onset time of ischemic events using the simply quantitative judgment of relative signal intensity (rSI) from various MR images. However, no uniform opinion has been achieved broadly till now. The controversy might derive from the potential patients’ selection bias of clinical retrospective study, the discrepant MR parameters, and the various sample sizes among different studies. Thus, we evaluated the temporal change of the relative DWI signal intensity (rDWI), relative ADC value (rADC), relative FLAIR signal intensity (rFLAIR), and relative T2 signal intensity (rT2), and further compare their diagnostic value in identifying the hyperacute lesions based on our embolic canine model with clear onset time. Twenty ischemic models were successfully established. All rSI values were linearly correlated to time with significance until 24 h after model establishment (P < 0.05). Paired comparison of ROC curves showed that significant difference was found between rADC and other three rSIs (P < 0.0001). However, no significant difference was found among rDWI, rT2 and rFLAIR. Our results indicated that rDWI, rFLAIR and rT2 may be helpful to predict the onset time of ischemic events with the similar diagnostic value. However, the rADC does not have comparable predictive value in our embolic canine model.
- Published
- 2014
43. Temporal evolution of the signal intensity of hyper-acute ischemic lesions in a canine stroke model: influence of hyperintense acute reperfusion marker
- Author
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Hai-Bin Shi, Qing-Quan Zu, Chen-Jiang Wu, Qian-Qian Gao, Xing-Long Liu, Xiao-Quan Xu, Sheng Liu, and Shan-Shan Lu
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Fluid-attenuated inversion recovery ,030218 nuclear medicine & medical imaging ,Brain Ischemia ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,Dogs ,medicine ,Effective diffusion coefficient ,Animals ,Radiology, Nuclear Medicine and imaging ,Embolization ,Middle cerebral artery occlusion ,Stroke ,business.industry ,Infarction, Middle Cerebral Artery ,medicine.disease ,Surgery ,Clinical Practice ,Disease Models, Animal ,Diffusion Magnetic Resonance Imaging ,Reperfusion Injury ,Signal intensity ,Nuclear medicine ,business ,030217 neurology & neurosurgery - Abstract
To assess the influence of the hyperintense acute reperfusion marker (HARM) on the relative signal intensity (rSI) and apparent diffusion coefficient (ADC) of hyper-acute ischemic lesions in a canine stroke model. Middle cerebral artery occlusion models were established using autologous clot embolization. Diffusion-weighted (DW) and fluid-attenuated inversion recovery (FLAIR) imaging was performed at 1, 2, 3, 4.5 and 6 h after embolization. HARM was defined as the delayed enhancement of cerebrospinal fluid on the subsequent FLAIR images after contrast media used. Twenty-four stroke models were successfully established and divided into the HARM (n = 16) and No-HARM group (n = 8). No significant differences were found in the rSI on DWI (b0 and b1000 map) and relative ADC between the two groups at each time point after embolization (all P > 0.05). There were no significant differences in the rSI on FLAIR at 1 and 2 h after embolization between the two groups (P > 0.05), while the HARM group showed significantly higher rSI on FLAIR than the No-HARM group at 3, 4.5 and 6 h after embolization (P = 0.044, 0.036 and 0.001, respectively). HARM should be noted during the quantitative analysis of FLAIR images in future clinical practice.
- Published
- 2016
44. An endovascular canine stroke model: middle cerebral artery occlusion with autologous clots followed by ipsilateral internal carotid artery blockade
- Author
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Qing-Quan Zu, Lei Sun, Hai-Bin Shi, Shan-Shan Lu, Sheng Liu, and Xiao-Quan Xu
- Subjects
Male ,Middle Cerebral Artery ,Pathology and Forensic Medicine ,Dogs ,medicine.artery ,Edema ,Occlusion ,medicine ,Animals ,cardiovascular diseases ,Molecular Biology ,Stroke ,Neurologic Examination ,medicine.diagnostic_test ,business.industry ,Angiography ,Brain ,Magnetic resonance imaging ,Cell Biology ,medicine.disease ,Magnetic Resonance Imaging ,Disease Models, Animal ,medicine.anatomical_structure ,Cerebral cortex ,Anesthesia ,Middle cerebral artery ,cardiovascular system ,Internal carotid artery ,medicine.symptom ,business ,Carotid Artery, Internal - Abstract
Stroke is one of the leading causes of death worldwide and the main reason for long-term disability. An appropriate animal model of stroke is urgently required for understanding the exact pathophysiological mechanism of stroke and testing any new therapeutic regimen. Our work aimed to establish a canine stroke model occluding the middle cerebral artery (MCA) and blocking the ipsilateral internal carotid artery (ICA), and to assess the infarct lesions by magnetic resonance imaging. The stroke model was generated by injecting two autologous clots into each MCA, followed by 2-h ipsilateral ICA blockade (ilICAB) using a catheter in 15 healthy adult beagles. Outcome measurements included 24-h and 7-day postocclusion T2-weighted imaging (T2WI)-based infarct volume calculation. In addition, pial collateral score, canine neurobehavioral score and histopathologic results were documented. Out of 15 dogs, 12 with successful MCA occlusion (MCAO) and ilICAB survived 7 days without complications or casualties and MCA were reperfused at 7 days after occlusion. High signal intensity in the basal ganglia and cerebral cortex on T2WI was initially observed in each dog at 6 h after the procedure. The mean percentage hemispherical infarct volume corrected for edema in all dogs on T2WI at 24 h after occlusion was 12.99±1.57%, and the degree of variability was 12.08%. The infarct volumes at 24 h after occlusion correlated with pial collateral scores and canine neurobehavioral scores well. This canine stroke model with combined MCAO and ilICAB reported here were proven to be highly feasible and reproducible.
- Published
- 2013
45. Minimally invasive salvage therapy for transplanted renal allografts
- Author
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Hai-Bin Shi, Jin-Guo Xia, Bin Wang, Sheng Liu, Lin-Bo Zhao, Qing-Quan Zu, Min Gu, Chun-Gao Zhou, and Xing-Long Liu
- Subjects
Adult ,Male ,medicine.medical_specialty ,Percutaneous ,Adolescent ,medicine.medical_treatment ,Renal Artery Obstruction ,Salvage therapy ,urologic and male genital diseases ,Critical Care and Intensive Care Medicine ,chemistry.chemical_compound ,Young Adult ,Postoperative Complications ,medicine ,Humans ,Transplantation, Homologous ,Kidney transplantation ,Nephrostomy, Percutaneous ,Retrospective Studies ,Salvage Therapy ,Creatinine ,business.industry ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Kidney Transplantation ,Surgery ,surgical procedures, operative ,Treatment Outcome ,chemistry ,Percutaneous nephrostomy ,Nephrology ,Female ,Stents ,Radiology ,Renal vein ,business ,Aneurysm, False ,Angioplasty, Balloon - Abstract
To evaluate the effectiveness of interventional therapy for complications of transplanted renal allografts.Between January 2009 and March 2014, 14 patients underwent interventional therapy for complications of renal allografts. Complications included transplant renal artery stenosis (TRAS), TRAS combined with pseudoaneurysms, transplant renal venous kinking and ureteral obstruction (UO). Serum creatinine (S.Cr) levels were evaluated before and after procedure. The characteristics and procedure outcomes of these patients with vascular and nonvascular complications were also analyzed.All primary procedures were successfully performed, which included percutaneous transluminal angioplasty (PTA) for TRAS (n = 4), stenting and coil embolization for TRAS combined with pseudoaneurysms (n = 1), stenting for renal vein kinking (n = 2), and percutaneous nephrostomy (PCN) for UO (n = 7) and secondary antegrade stent placement in six UO patients after 1 week of PCN. No major procedure related complications occurred. S.Cr level subsequently improved from 6.0 ± 3.6 to 2.6 ± 2.1 mg/dL (p 0.001), as well as patients' clinical features within 1 week after procedure. In our study, the onset time of vascular complications was earlier (6 months) than nonvascular complications with significant difference (p 0.001). During follow-up, the patient with TRAS and pseudoaneurysms suffered acute rejection 1 month after treatment and received transplant renal artery embolization. One patient with TRAS showed restenosis 4 months after procedure, and was retreated successfully with stenting. Thirteen cases reserved their transplanted renal allografts.Interventional therapy could be prior considered for transplanted renal allograft complications as its effectiveness and minimal invasiveness in saving the transplanted renal grafts.
- Published
- 2015
46. Simple quantitative measurement based on DWI to objectively judge DWI-FLAIR mismatch in a canine stroke model
- Author
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Hai-Bin Shi, Gaojun Teng, Qiguang Cheng, Bin Wang, Xiaoquan Xu, Qing-Quan Zu, Shan-Shan Lu, Jing Yu, Xing-Long Liu, and Sheng Liu
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,Fluid-attenuated inversion recovery ,medicine.disease ,Surgery ,Stroke ,Disease Models, Animal ,Diffusion Magnetic Resonance Imaging ,Dogs ,Left middle cerebral artery ,Neuroradiology ,Ischemic stroke ,medicine ,Effective diffusion coefficient ,Animals ,Radiology, Nuclear Medicine and imaging ,Signal intensity ,Cardiology and Cardiovascular Medicine ,Nuclear medicine ,business ,Retrospective Studies - Abstract
PURPOSE Diffusion-weighted imaging (DWI) - fluid attenuated inversion recovery (FLAIR) mismatch was proven useful to time the onset of wake-up stroke; however, identifying the status of FLAIR imaging has been mostly subjective. We aimed to evaluate the value of relative DWI signal intensity (rDWI), and relative apparent diffusion coefficient (rADC) in identifying the FLAIR status in the acute period. METHODS Autologous clot was used to embolize left middle cerebral artery in 20 dogs. Magnetic resonance imaging was performed 3-6 hours and 24 hours after embolization. DWI-FLAIR mismatch was defined as hyperintense signal detected on DWI, but not on FLAIR. The mean values of rDWI or rADC of FLAIR- and FLAIR+ lesions were compared and the critical cutoff values of rDWI and rADC for identifying the FLAIR status were determined. RESULTS Stroke models were successfully established in all animals. DWI+ lesions were found in all 20 dogs from three hours, while FLAIR+ lesions were found in three, 11, 16, 19, and 20 dogs at five time points after embolization, respectively. The mean rDWI values were significantly different between FLAIR- and FLAIR+ lesions (P < 0.001), but rADC values were not (P = 0.73). Using rDWI=1.90 as the threshold value, excellent diagnostic efficacy was achieved (AUC, 0.88; sensitivity, 0.77; specificity, 0.88). However, rADC appeared not useful (AUC, 0.48; sensitivity, 0.52; specificity, 0.58) in identifying the FLAIR status. CONCLUSION In our embolic canine stroke model, rDWI was useful to identify FLAIR imaging status in the acute period, while rADC was not.
- Published
- 2015
47. G-CSF and hypoxic conditioning improve the proliferation, neural differentiation and migration of canine bone marrow mesenchymal stem cells
- Author
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Shan‑Shan Lu, Qi‑Guang Cheng, Xiao‑Quan Xu, Jing Yu, Sheng Liu, Xing‑Long Liu, and Qing‑Quan Zu
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,Ischemia ,Regenerative medicine ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,stomatognathic system ,Medicine ,Viability assay ,business.industry ,hemic and immune systems ,General Medicine ,Articles ,medicine.disease ,Granulocyte colony-stimulating factor ,Transplantation ,030104 developmental biology ,medicine.anatomical_structure ,Apoptosis ,Immunology ,Cancer research ,business ,030217 neurology & neurosurgery ,Homing (hematopoietic) - Abstract
Transplantation using bone marrow mesenchymal stem cells (BMSCs) is emerging as a potential regenerative therapy after ischemic attacks in the brain. However, it has been questioned because very few transplanted BMSCs are detected homing to and survived in the ischemic region. Improving the cell viability and migration ability under the complex ischemic condition seems very important. The aim of our study is to identify whether hypoxic condition and granulocyte colony-stimulating factor (G-CSF) could improve the cell survival and migration ability of transplanted cells or hypoxic condition could promote BMSC's neural differentiation. BMSCs were treated under either normoxic (21% O2) or hypoxic (1% O2) (HP-BMSCs) conditions, no significant apoptosis was observed in hypoxic precondition (HP) group, our study confirmed that HP improves BMSCs proliferation and migration. Meanwhile, neural induction of BMSCs under hypoxic condition exhibited significant superior results than normoxic condition. Additionally, the addition of G-CSF in HP-BMSCs culture media promoted HP efficiency on BMSCs. These findings shed light on novel efficient strategy on the prosperity of BMSCs. Hypoxic preconditioning and cultured with G-CSF may become a promising therapeutics for cell-based therapy in the treatments of ischemia stroke.
- Published
- 2015
48. Percutaneous transpapillary biliary stent placement for distal malignant biliary obstruction: Outcomes and survival analysis.
- Author
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Qing-Quan Zu, Jin-Xing Zhang, Bin Wang, Wei Ye, Sheng Liu, and Hai-Bin Shi
- Published
- 2019
- Full Text
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49. Correlation between Intravoxel Incoherent Motion Magnetic Resonance Imaging Derived Metrics and Serum Soluble CD40 Ligand Level in an Embolic Canine Stroke Model
- Author
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Sheng Liu, Qing-Quan Zu, Chen-Jiang Wu, Qian-Qian Gao, Xing-Long Liu, Xiao-Quan Xu, Hai-Bin Shi, and Shan-Shan Lu
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Male ,medicine.medical_specialty ,Imaging biomarker ,CD40 Ligand ,030218 nuclear medicine & medical imaging ,Neuroimaging and Head & Neck ,Correlation ,03 medical and health sciences ,Dogs ,Magnetic resonance imaging ,0302 clinical medicine ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Stroke ,Intravoxel incoherent motion ,Experimental study ,medicine.diagnostic_test ,business.industry ,Brain ,medicine.disease ,Ligand (biochemistry) ,IVIM ,Disease Models, Animal ,Diffusion Magnetic Resonance Imaging ,Female ,Original Article ,Diffusion-weighted imaging ,Radiology ,Nuclear medicine ,business ,Perfusion ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
Objective To determine the relationship between intravoxel incoherent motion (IVIM) imaging derived quantitative metrics and serum soluble CD40 ligand (sCD40L) level in an embolic canine stroke model. Materials and Methods A middle cerebral artery occlusion model was established in 24 beagle dogs. Experimental dogs were divided into low- and high-sCD40L group according to serum sCD40L level at 4.5 hours after establishing the model. IVIM imaging was scanned at 4.5 hours after model establishment using 10 b values ranging from 0 to 900 s/mm2. Quantitative metrics diffusion coefficient (D), pseudodiffusion coefficient (D*), and perfusion fraction (f) of ischemic lesions were calculated. Quantitative metrics of ischemic lesions were normalized by contralateral hemisphere using the following formula: normalized D = Dstroke / Dcontralateral. Differences in IVIM metrics between the low- and high-sCD40L groups were compared using t test. Pearson's correlation analyses were performed to determine the relationship between IVIM metrics and serum sCD40L level. Results The high-sCD40L group showed significantly lower f and normalized f values than the low-sCD40L group (f, p < 0.001; normalized f, p < 0.001). There was no significant difference in D*, normalized D*, D, or normalized D value between the two groups (All p > 0.05). Both f and normalized f values were negatively correlated with serum sCD40L level (f, r = −0.789, p < 0.001; normalized f, r = −0.823, p < 0.001). However, serum sCD40L level had no significant correlation with D*, normalized D*, D, or normalized D (All p > 0.05). Conclusion The f value derived from IVIM imaging was negatively correlated with serum sCD40L level. f value might serve as a potential imaging biomarker to assess the formation of microvascular thrombosis in hyperacute period of ischemic stroke.
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- 2017
50. Multimodal magnetic resonance imaging for assessing lacunar infarction after proximal middle cerebral artery occlusion in a canine model
- Author
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Shan-Shan, Lu, Sheng, Liu, Qing-Quan, Zu, Xiao-Quan, Xu, Jian-Wei, Wang, Jing, Yu, Lei, Sun, and Hai-Bin, Shi
- Subjects
Male ,Diffusion Magnetic Resonance Imaging ,Dogs ,Stroke, Lacunar ,Animals ,Infarction, Middle Cerebral Artery ,Magnetic Resonance Angiography - Abstract
A new lacunar infarction model was recently established in beagle dogs through proximal middle cerebral artery (MCA) occlusion by thrombus. This study aimed to characterize the model by multimodal magnetic resonance imaging (MRI) and to investigate its potential role for the future stroke research.The left proximal MCA was embolized with an autologous thrombus in six beagles. Diffusion-weighted imaging (DWI) and T2-weighted imaging (T2WI) were performed every half hour during the first six hours after occlusion, followed by three time points at 12 hours, 24 hours, and one week. Perfusion-weighted imaging (PWI) and magnetic resonance angiography (MRA) were carried out at six hours, 24 hours and one week. The PWI-DWI mismatch ratio was defined as (PWI-DWI)/DWI ischemic volume.Lacunar infarcts induced by MCA occlusion were located in the left caudate nucleus and internal capsule. All the lesions could be detected within two hours by DWI. Lesion volume on DWI increased in a time dependent manner, from (87.19 ± 67.16) mm(3) at one hour up to (368.98 ± 217.05) mm(3) at 24 hours (P = 0.009), while that on PWI gradually decreased from (7315.00 ± 2054.38) mm(3) at six hours to (4900.33 ± 1319.71) mm(3) at 24 hours and (3334.33 ± 1195.11) mm(3) at one week (P = 0.002). The mismatch ratio was 41.93 ± 22.75 at six hours after ischemia, showing "extensive mismatch", and decreased to 18.10 ± 13.74 at 24 hours (P = 0.002). No MCA recanalization was observed within 24 hours after MCA occlusion.Lacunar infarction induced by proximal MCA occlusion could be detected early by DWI and was characterized by extensive PWI-DWI mismatch. Multimodal MRI is useful to demonstrate the natural evolution of PWI-DWI mismatch. This ischemic model could be further used for investigating early thrombolysis in lacunar stroke showing extensive mismatch.
- Published
- 2013
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