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In this study, we investigated the growth performance and early sex differentiation in Chinese longsnout catfish (Leiocassis longirostris), and further systematically evaluated its feminization by oral administration of various doses and treatment durations of 17β-estradiol (E2). Under the same breeding condition, the growth rate of male Chinese longsnout catfish was 20.8 % and 33.9 % faster than that of the female sibling at one- and two-year-old, respectively. The ovarian cavity began forming on 15 dph and completed by 21 dph, while the primary oocytes were at chromatin-nucleolus stage by 33 dph and at the peri-nucleolus stage oocytes by 65 dph. In presumptive testis, the seminiferous lobule appeared on 15 dph and the efferent duct anlage presented on 53 dph. Subsequently, germ cells entered into mitosis and formed the spermatogonia at 69 dph. Compared with the control group (no E2 diet), the fries treated with E2 at 10, 50, 100 or 150 mg/kg diet from 10 to 90 dph were 100 % female by morphology and histology observation. However, the growth and survival rate rapidly decreased as the E2 dosage rose. Furthermore, fries treated with10 mg/kg E2 during 15–85 dph, 20–80 dph, 25–75 dph, 30–70 dph, 35–65 dph and 40–60 dph were explored. 3.33 ± 3.33 % and 45.55 ± 5.09 % sex reversal ratio was identified in 30–70 dph and 35–65 dph group, respectively. All female populations were observed in 15–85 dph, 20–80 dph and 25–75 dph groups, despite slow development of ovary among them compared with the control group. Additionally, the growth performance of fries in 25–75 dph group was closest to that of the control group, while their serum E2 and 11-KT levels were consistent with XX females. Transcriptomic analyses were performed to assess gene expression difference among the XX females, XY males and feminized XY females, and most DEGs were shared between XY male vs XX female and XY male vs XY female. In addition, qRT-PCR validation of 12 DEGs associated with gonadal differentiation were consistent with RNA-Seq analysis (r=0.942, P

AimThe Triglyceride-Glucose (TyG) index, an indicator of insulin resistance, has been proposed as a predictor of cardiovascular diseases. However, its role in predicting stroke risk, particularly in low-income populations, is not well understood. This study aimed to investigate the predictive value of the TyG index for stroke incidence in a low-income Chinese population, with a focus on gender and age-specific differences.MethodsThis 10-year prospective cohort study included 3,534 participants aged ≥45 years from rural areas in northern China. Baseline data on demographic characteristics, lifestyle factors, and clinical measurements were collected. Participants were followed for stroke incidence, categorized into ischemic and hemorrhagic stroke. Multivariate logistic regression models were used to assess the association between the TyG index and stroke incidence, adjusting for potential confounders.ResultsDuring the follow-up period, 368 participants (10.4%) experienced a stroke, with 327 ischemic and 31 hemorrhagic strokes. TyG index was significantly associated with total and ischemic stroke incidence but not hemorrhagic stroke. After adjusting for confounding factors, for every one standard deviation increase in TyG index, the risk of stroke increased by 32% for overall stroke (RR: 1.32; 95% CI: 1.08-1.61; P=0.006) and 39% for ischemic stroke (RR: 1.39; 95% CI: 1.12-1.73; P=0.003). The risk of stroke in the highest TyG tertile levels (tertile 3) increased by 49% (RR: 1.49; 95% CI 1.11-1.99; P=0.007) for overall stroke, compared to those in the lowest tertile levels (tertile 1). For ischemic stroke, the risk of stroke increased by 53% (RR: 1.53; 95% CI 1.12-2.11; P=0.008) in the highest TyG tertile levels (tertile 3) compared to those in the lowest tertile levels (tertile 1).ConclusionThis 10-year prospective cohort study has established the TyG index as an independent predictor of both total and ischemic stroke incidence in a low-income Chinese population. The findings indicate that the TyG index is particularly effective in predicting stroke risk among women and older adults (≥60 years), but not for hemorrhagic stroke. These insights are crucial for improving clinical practice and stroke prevention strategies.

Background: Hydrogen gas and microalgae both exist in the natural environment. We aimed to integrate hydrogen gas and biology nano microalgae together to expand the treatment options in sepsis. Methods: Phosphoproteomics, metabolomics and proteomics data were obtained from mice undergoing cecum ligation and puncture (CLP) and inhalation of hydrogen gas. All omics analysis procedure were accordance with standards. Multi R packages were used in single cell and spatial transcriptomics analysis to identify primary cells expressing targeted genes, and the genes’ co-expression relationships in sepsis related lung landscape. Then, network pharmacology method was used to identify candidate drugs. We used hydrophobic-force-driving self-assembly method to construct dihydroquercetin (DQ) nanoparticle. To cooperate with molecular hydrogen, ammonia borane (B) was added to DQ surface. Then, Chlorella vulgaris (C) was used as biological carrier to improve self-assembly nanoparticle. Vivo and vitro experiments were both conducted to evaluate anti-inflammation, anti-ferroptosis, anti-infection and organ protection capability. Results: As a result, we identified Esam and Zo-1 were target phosphorylation proteins for molecular hydrogen treatment in lung. Ferroptosis and glutathione metabolism were two target pathways. Chlorella vulgaris improved the dispersion of DQB and reconstructed morphological features of DQB, formed DQB@C nano-system (size = 307.3 nm, zeta potential = −22mv), with well infection-responsive hydrogen release capability and biosafety. In addition, DQB@C was able to decrease oxidative stress and inflammation factors accumulation in lung cells. Through increasing expression level of Slc7a11/xCT and decreasing Cox2 level to participate with the regulation of ferroptosis. Also, DQB@C played lung and multi organ protection and anti-inflammation roles on CLP mice. Conclusion: Our research proposed DQB@C as a novel biology nano-system with enormous potential on treatment for sepsis related acute lung injury to solve the limitation of hydrogen gas utilization in clinics.

In this research, a pesticide-fertilizer combination (PFC) was fabricated using chlorantraniliprole and biochar. The PFC had a spherical shape, smooth surface, uniform particles, relatively dense structure and good compression resistance. The adsorption propertie of biochar to chlorantraniliprole was investigated. During the preparation of pelleted granules, the types and concentrations of core fillers and adhesives were screened and optimized. Besides, the safety to maize and effectiveness against fall armyworms of PFC had been investigated. Meanwhile, The PFC could promoted growth of maize significantly and control fall armyworm effectively. This work provides a promising approach to slow the release of chlorantraniliprole, which has a potential application to enhance the pesticides efficiency.

The lap welding process for 304L stainless steel welded using the pulsed gas tungsten arc welding (P-GTAW) procedure was studied, and the effects of the pulse welding parameters (the peak current, background current, duty cycle, pulse frequency, and welding speed) on the macroscopic morphology, microstructure, and mechanical properties of the resultant lap joints were investigated. Tensile tests, hardness measurements, and SEM/EDS/XRD analyses were conducted to reveal the characterization of the joint. The relationships between the welding parameters; certain joint characteristic dimensions (the weld width, D; the weld width on the lower plate, La; the weld depth on the lower plate, P; and the minimum fusion radius, R); and the maximum tensile bearing capacity were studied. The weld zone was primarily composed of vermicular ferrite, skeletal ferrite, and austenite, and no obvious welding defects, precipitation, or phase transformations were evident in the weld. Microhardness tests demonstrated that the weld microhardness was highest in the base metal zone and lowest in the weld zone. As the heat input increased, the average microhardness decreased. The hardness difference reached 17.6 Hv10 due to the uneven grain size and the transformation of the structure to ferrite in the weld. The fracture location in welded joints varied as the heat input changed. In some parameter combinations, the weld tensile strength was significantly higher than that of the base material, with fractures occurring in the weld. Scanning electron microscopy results exhibited an obvious dimple morphology, which is a typical form of ductile fracture. XRD revealed no significant phase changes in the weld zone, with a higher intensity of the austenite diffraction peaks compared to the ferrite diffraction peaks.

Abstract Background The excellent physicochemical and biomedical properties make silk fibroin (SF) suitable for the development of biomedical materials. In this research, the silk fibroin microspheres (SFMS) were customized in two size ranges, and then carried gold nanoparticles or doxorubicin to evaluate the performance of drug loading and releasing. Embolization efficiency was evaluated in rat caudal artery and rabbit auricular artery, and the in vivo distribution of iodinated SFMS (125I/131I-SFMS) after embolization of rat hepatic artery was dynamically recorded by SPECT. Transhepatic arterial radioembolization (TARE) with 131I-SFMS was performed on rat models with liver cancer. The whole procedure of selective internal radiation was recorded with SPECT/CT, and the therapeutic effects were evaluated with 18 F-FDG PET/CT. Lastly, the enzymatic degradation was recorded and followed with the evaluation of particle size on clearance of sub-micron silk fibroin. Results SFMS were of smooth surface and regular shape with pervasive pores on the surface and inside the microspheres, and of suitable size range for TAE. Drug-loading functionalized SFMS with chemotherapy or radio-sensitization, and the enhanced therapeutic effects were proved in treating HUH-7 cells as lasting doxorubicin release or more lethal radiation. For artery embolization, SFMS effectively blocked the blood supply; when 131I-SFMS serving as the embolic agent, the good labeling stability and embolization performance guaranteed the favorable therapeutic effects in treating in situ liver tumor. At the 5th day post TARE with 37 MBq/3 mg 131I-SFMS per mice, tumor activity was quickly inhibited to a comparable glucose metabolism level with surrounding normal liver. More importantly, for the fragments of biodegradable SFMS, smaller sized SF ( 800 nm) entered the liver within 72 h for further metabolism. Conclusion The feasibility of SFMS as degradable TARE agent for liver cancer was primarily proved as providing multiple therapeutic potentials. Graphical abstract

The security and stability control system is the guarantee of the security and stability operation of the power grid. With the increasing scale of distributed new energy access to the power grid, the security and stability control strategy of the power grid is becoming more complex, and it is becoming increasingly important to correctly analyze and implement the security and stability control strategy. In order to ensure the correctness of the security and stability control strategy implemented by the security and stability control device, it is necessary to analyze the security and stability control strategy in detail. Therefore, this article proposes an intelligent analysis method of the security and stability control strategy based on the knowledge graph. First, this article introduces the ontology design method of the security and stability control strategy based on the knowledge graph, combines the characteristics and applications of the knowledge graph, analyzes the relationship between the elements of the strategy, and designs a clear-structured knowledge network. Second, this article analyzes the automatic construction technology of the graph, constructs the six-element ontology model of the security and stability control strategy, and realizes the human–computer interaction functions such as auxiliary decision making, strategy reasoning, and intelligent search based on the knowledge graph. Using artificial intelligence technology, this article takes the security and stability control strategy of a certain area’s security and stability control system as an example to model and manage. The results show that it can assist the tester to quickly retrieve the strategy, effectively improve the detection efficiency of the security and stability control strategy, avoid the omission and ambiguity caused by the manual understanding of the strategy, and ensure the accuracy and comprehensiveness of the security and stability control strategy detection.

As a typical micro/nano processing technique, femtosecond laser fabrication provides the opportunity to achieve delicate microstructures. The outstanding advantages, including nanoscale feature size and 3D architecting, can bridge the gap between the complexity of the central nervous system in virto and in vivo. Up to now, various types of microstructures made by femtosecond laser are widely used in the field of neurobiological research. In this mini review, we present the recent advancement of femtosecond laser fabrication and its emerging applications in neurobiology. Typical structures are sorted out from nano, submicron to micron scale, including nanoparticles, micro/nano-actuators, and 3D scaffolds. Then, several functional units applied in neurobiological fields are summarized, such as central nervous system drug carriers, micro/nano robots and cell/tissue scaffolds. Finally, the current challenges and future perspective of integrated neurobiology research platform are discussed.

Biomimetic switchable adhesion interfaces (BSAIs) with dynamic adhesion states have demonstrated significant advantages in micro-manipulation and bio-detection. Among them, gecko-inspired adhesives have garnered considerable attention due to their exceptional adaptability to extreme environments. However, their high adhesion strength poses challenges in achieving flexible control. Herein, we propose an elegant and efficient approach by fabricating three-dimensional mushroom-shaped polydimethylsiloxane (PDMS) micropillars on a flexible PDMS substrate to mimic the bending and stretching of gecko footpads. The fabrication process that employs two-photon polymerization ensures high spatial resolution, resulting in micropillars with exquisite structures and ultra-smooth surfaces, even for tip/stem ratios exceeding 2 (a critical factor for maintaining adhesion strength). Furthermore, these adhesive structures display outstanding resilience, enduring 175% deformation and severe bending without collapse, ascribing to the excellent compatibility of the micropillar’s composition and physical properties with the substrate. Our BSAIs can achieve highly controllable adhesion force and rapid manipulation of liquid droplets through mechanical bending and stretching of the PDMS substrate. By adjusting the spacing between the micropillars, precise control of adhesion strength is achieved. These intriguing properties make them promising candidates for various applications in the fields of microfluidics, micro-assembly, flexible electronics, and beyond.

The probability of rhabdomyosarcoma occurring in the cervix is less than 0.5% and may be associated with a pathogenic dicer 1, ribonuclease III ( DICER1 ) gene variation. Tumour-induced hypercoagulability and high levels of cancer antigen (CA) 125 are risk factors for cerebral venous sinus thrombosis (CVST). In addition, although nonpuerperal uterine inversion is very rare and is usually caused by leiomyomas from the uterus, large cervical masses can also be the cause. This case report describes a 24-year-old woman with uterine inversion caused by an embryonic cervical rhabdomyosarcoma that presented with CVST as her first symptom. The patient underwent laparoscopic total uterus and bilateral salpingectomy, during which the uterus was found to be completely inverted. Postoperative pathology confirmed embryonic cervical rhabdomyosarcoma. The patient quickly developed lung and para-aortic lymph node metastases. Two months later, the patient died of complications. When coagulation indices in patients with tumours are abnormal, especially when the levels of D-dimer and CA125 increase, it is recommended that anticoagulant therapy is administered in a timely manner to prevent the occurrence of CVST. Furthermore, for large cervical tumours, physicians should also be alert to the occurrence of uterine inversion.

In order to further study the properties of boundary value problems for fractional differential equations with non-linear terms,we discusses the existence of positive solutions for eigenvalue problems of (n-1,1) fractional differential equations with sign-changing nonlinear terms, where the fractional derivative is Riemann-Liouville. First we use the Green function for a given boundary value problem and convert the differential equation into an equivalent integral equation. Then, under the condition that the nonlinear term f(t, x) satisfies Caratheodory conditions (that is to say, the non-linear term f(t,x) is measurable function when the variable x was chosen arbitrarily, and the non-linear term f(t,x) is continuous function of x when the variable t was fixed). By constructing a suitable Banach space, we use the cone-stretching and compression fixed point theorem and Leray-Schauder nonlinear selection to obtain the sufficient conditions for the existence of positive solutions of boundary value problems.The results show that the nonlinear term f(t,x) can be singular at any point t. At the same time, the range of the eigenvalue which makes the solution of the boundary value problem exist was changed.The results lay a foundation for further study of the existing conclusions.

To ensure the communication safety of unmanned underwater vehicle,a physical layer key generation scheme of underwater acoustic channel based on orthogonal frequency division multiplexing (OFDM) system was proposed.First,a local pilot assistance channel sounding protocol was proposed to solve the reciprocity impaired caused by the great propagation delay in the underwater acoustic channel,ensure high key randomness and enhance the defenses against nearby eavesdroppers.Secondly,double-layer compensation centralized combined with adaptive guard interval quantization method was proposed to improve key agreement rate and key generation rate.Simulation results show that the scheme effectively overcomes the problems of impaired reciprocity and ensures high key generation rate and high randomness on the premise that the key agreement is superior to the existing schemes.

A gelator 1 containing functional quinoline and Schiff base groups that could form organogels in DMF, DMSO, acetone, ethanol and 1,4-dioxane was designed and synthesized. The self-assembly process of geator 1 was characterized by field emission scanning electron microscopy (FESEM), UV-vis absorption spectroscopy, fluorescence emission spectroscopy, Fourier transform infrared spectroscopy(FTIR), X-ray powder diffraction (XRD) and water contact angle. Under non-covalent interactions, gelator 1 self-assembled into microbelts and nanofiber structures with different surface wettability. Weak fluorescence was emitted from the solution and gel state of 1. Interestingly, gelator 1 exhibited good selectivity and sensitivity towards Zn2+ in solution and gel states along with its emission enhancement and change. The emission intensity at 423 nm of solution 1 in 1,4-dioxane was slightly enhanced, and a new emission peak appeared at 545 nm along with its intensity sequentially strengthened in the titration process. The obvious ratiometric detection process was presented with a limit of detection (LOD) of 5.51 μM. The detection mechanism was revealed by a theoretical calculation and NMR titration experiment, which was that Zn2+ induced the transition from trans- to cis- of molecule 1 and further coordinated with 1. This study will introduce a new method for the construction of functional self-assembly gel sensors for the detection of Zn2+.

A novel gelator (1) based on a bis-pyridine derivative was designed and synthesized, which could form stable gels in methanol, ethanol, acetonitrile, ethyl acetate, DMF/H2O (4/1, v/v) and DMSO/H2O (4/1, v/v). The self-assembly process of gelator 1 was studied by field emission scanning electron microscopy (FESEM), UV–vis absorption spectroscopy, fluorescence emission spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction and a water contact angle experiment. Gelator 1 exhibited obvious AIE behavior. On the base of its AIE, the gel of 1 could detect Hg2+, which resulted in fluorescence quenching and a gel–sol transition. 1H NMR titration experiments with Hg2+ revealed that the metal coordination interaction induced the fluorescence quenching and the breakdown of the noncovalent interaction in the gel system. This research provides a new molecular mode for designing a functional self-assembly gel system.

Characterization of the structure and pharmacological activity of Berberis dasystachya Maxim., a traditional Tibetan medicinal and edible fruit, has not yet been reported. In this study, central composite design (CCD) combined with response surface methodology (RSM) was applied to optimize the extraction conditions of B. dasystachya oil (BDSO) using the supercritical carbon dioxide (SC-CO2) extraction method, and the results were compared with those obtained by the petroleum ether extraction (PEE) method. The chemical characteristics of BDSO were analyzed, and its antioxidant activity and in vitro cellular viability were studied by DPPH, ABTS, reducing power assay, and MTT assay. The results showed that the maximum yield of 12.54 ± 0.56 g/100 g was obtained at the optimal extraction conditions, which were: pressure, 25.00 MPa; temperature 59.03 °C; and CO2 flow rate, 2.25 SL/min. The Gas chromatography (GC) analysis results showed that BDSO extracted by the SC-CO2 method had higher contents of unsaturated fatty acids (85.62%) and polyunsaturated fatty acids (57.90%) than that extracted by the PEE method. The gas chromatography used in conjunction with ion mobility spectrometry (GC–IMS) results showed that the main volatile compounds in BDSO were aldehydes and esters. BDSO also exhibited antioxidant ability in a dose-dependent manner. Moreover, normal and cancer cells incubated with BDSO had survival rates of more than 85%, which indicates that BDSO is not cytotoxic. Based on these results, the BDSO extracted by the SC-CO2 method could potentially be used in other applications, e.g., those that involve using berries of B. dasystachya.

The protection of video data has become a hot topic of research. Researchers have proposed a series of coding algorithms to ensure the safe and efficient transmission of video information. We propose an encryption scheme that can protect video information with higher security by combining the video coding algorithm with encryption algorithm. The H.264/AVC encoding algorithm encodes the video into multiple slices, and the slices are independent of each other. With this feature, we encrypt each slice while using the cipher feedback (CFB) mode of the advanced encryption standard (AES) with the dynamic key. The key is generated by the pseudo-random number generator (PRNG) and updated in real time. The encryption scheme goes through three phases: constructing plaintext, encrypting plaintext, and replacing the original bitstream. In our scheme, we encrypt the code stream after encoding, so it does not affect the coding efficiency. The purpose of the CFB mode while using the AES encryption algorithm is to maintain the exact same bit rate and produce a format compatible bitstream. This paper proposes a new four-dimensional (4-D) hyperchaotic algorithm to protect data privacy in order to further improve the security of video encryption. Symmetric encryption requires that the same key is used for encryption and decoding. In this paper, the symmetry method is used to protect the privacy of video data due to the large amount of video encrypted data. In the experiment, we evaluated the proposed algorithm while using different reference video sequences containing motion, texture, and objects.

Cytophaga hutchinsonii is a gliding cellulolytic bacterium that degrades cellulose in a substrate contact-dependent manner. Specific proteins are speculated to be translocated to its extracellular milieu or outer membrane surface to participate in adhesion to cellulose and further digestion. In this study, we show that three orthologous genes encoding the major components (T2S-D, -F, and -G) of type II secretion system (T2SS) are involved in cellulose degradation but not in cell motility. The individual disruption of the three t2s genes results in a significantly retarded growth on cellobiose, regenerated amorphous cellulose, and Avicel cellulose. Enzymatic analyses demonstrate that, whereas the endoglucanase activity of the t2s mutant cells is increased, the β-glucosidase activity is remarkably reduced compared to that of WT cells. Further analyses reveal that the t2s mutant cells not only exhibit a different profile of cellulose-bound outer membrane proteins from that of wild-type cells, but also display a significant decrease in their capability to adhere to cellulose. These results indicate that a functional link exits between the putative T2SS and cellulose utilization in C. hutchinsonii, and thus provide a conceptual framework to understand the unique strategy deployed by C. hutchinsonii to assimilate cellulose.