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Mengqi Zhang,1,* Shengxian Bao,2,* Guanhua Qiu,2,* Jingchen Liang,2 Qin Wang,2 Xiaoqi Zhu,2 Guchun Qin,1 Junjie Liu,2 Chang Zhao1 1Department of Interventional Therapy, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Ultrasound and Department of Radiology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junjie Liu; Chang Zhao, Email liujunjie197806@163.com; 710519137@qq.comPurpose: In this experiment, we constructed a magnetic targeting nano-diagnosis and treatment platform of doxorubicin (DOX) combined with iron nanoparticles, and explored their application value and mechanism in the treatment of Triple Negative Breast Cancer (TNBC), as well as its new diagnosis and treatment mode in Magnetic Resonance Imaging (MRI).Patients and Methods: Hollow mesoporous nanoparticles (HFON) were synthesized by solvothermal method, and loaded the drug DOX (DOX@HFON) to treat TNBC. The experiments in vivo and in vitro were carried out according to the characteristics of the materials. In vitro experiments, the killing effect of the drug on cells was verified by cell viability CCK8, ROS generation level, LPO evaluation and flow cytometry; the MRI effect and targeted anti-tumor therapy effect were studied by in vivo experiments; then the tumor tissue sections were detected by Ki-67, CD31, ROS, LPO and TUNEL immunofluorescence detection; H&E staining and blood biochemical tests were used to evaluate the biosafety of the materials.Results: Through a series of characterization tests, it is confirmed that the nano-materials prepared in this experiment have positive drug loading properties. MDA-MB-231 cells had great phagocytic ability to DOX@HFON under Confocal Laser Scanning Microscope (CLSM). Experiments in vitro confirmed that DOX and Fe were released and concentrated in cells, an

Cunqing Kong,1,* Banghao Xu,2,* Guanhua Qiu,1 Meng Wei,2 Mengqi Zhang,3 Shengxian Bao,1 Jiali Tang,1 Lequn Li,2 JunJie Liu1 1Department of Ultrasound, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, 530021, People’s Republic of China; 2Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, 530021, People’s Republic of China; 3Department of Interventional Therapy Affiliated Tumor Hospital of Guangxi Medical University, Nanning, 530021, People’s Republic of China*These authors contributed equally to this workCorrespondence: JunJie Liu, Department of ultrasound, Affiliated Tumor Hospital of Guangxi Medical University, 71 Hedi Road, Nanning, 530021, People’s Republic of China, Email liujunjie@gxmu.edu.cn Lequn Li, Department of hepatobiliary surgery, Affiliated Tumor Hospital of Guangxi Medical University, 71 Hedi Road, Nanning, 530021, People’s Republic of China, Email li_lequn@263.netIntroduction: Photoimmunotherapy is a breakthrough treatment for malignant tumors. Its uniqueness is that it uses antibody mediated targeted delivery to achieve high tumor specificity and uses laser-activated biophysical mechanism to accurately induce the rapid death of cancer cells and avoid damaging the surrounding normal tissues.Methods: In this paper, an iron-based micelle was designed to encapsulate the photothermal agent indocyanine green (ICG) and a cyclic tripeptide of arginine-glycine-aspartic acid (cRGD) as targeted multifunctional ICG@SANPs-cRGD nanoparticles for combined photothermal/photodynamic/immune therapy of breast cancer.Results: The experimental results show that ICG@SANPs-cRGD nanoparticles have good biocompatibility and photothermal conversion ability. Photothermal therapy (PTT) and photodynamic therapy (PDT) based on ICG@SANPs-cRGD can not only inhibit the proliferation, invasion and migration of tumor cells, but also directly kill tumor cells by inducing apoptosis or

Zhijun Jiang,1,* Yu Zhang,1,* Xinyu Liu,1 Jingchen Liang,2 Guanhua Qiu,2 Xiaoqi Zhu,2 Jie Chen,1 Lequn Li1 1Department of Hepatobiliary Surgery, Guangxi Medical University Cancer Hospital, Nanning, People’s Republic of China; 2Department of Ultrasound, Guangxi Medical University Cancer Hospital, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lequn Li; Jie Chen Department of Hepatobiliary SurgeryGuangxi Medical University Cancer Hospital, Nanning, People’s Republic of ChinaTel +86 771 533 0855Fax +86 771 531 2000Email lilequn82@163.com; jiechen185@163.comPurpose: To establish a novel circRNA–miRNA–mRNA network associated with the poor prognosis of hepatocellular carcinoma (HCC).Materials and Methods: Quantitative real-time PCR was used to verify the differentially expressed circRNA. Moreover, the competing endogenous RNA networks were established using bioinformatics methods. Meanwhile, the prognostic value and potential mechanism of ceRNA network in hepatocellular carcinoma (HCC) were analyzed.Results: This work found that circ_0130911 was highly expressed in HCC tissues and early recurring HCC. Further, we effectively constructed a ceRNA network. The ceRNA network regulated by circ_0130911 might influence the prognosis of HCC by regulating cell cycle-related pathways.Conclusion: The ceRNA network proposed here can be used as a novel biomarker for the prognosis of HCC, thereby providing new insights for the targeted therapy of HCC.Keywords: circRNAs, ceRNAs, biomarker, hepatocellular carcinoma

This thesis constructs two Computable General Equilibrium (CGE) world trade models, and examines the empirical evidence on whether the European Union is more governed by a Classical model of comparative advantage or a Gravity model of new trade theory . It applies time series data between 1970 and 2018 and aims to test the models using Indirect Inference Wald (IIW) on EU trade facts. It extends the Minford and Xu s (2018) UK study, treats EU as a large open economy and simulates worl d variables by using a reduced form VAR of the unknown true world model , applying a Part of Model test. Empirically , the Classical model pass es the tests comfortably whilst the Gravity modelpass es when test power and gravity effect is low, but cannot surv ive in the full test power . Based on a Monte Carlo experiment , we find that the auxiliary model creates substantial but not excessive power for the test . The policy implication of our work is that both models suggest that European Union policymakers should liberaliz e trade policy, because under both model tariff simulations indicate that protection results in general welfare loss.

This thesis constructs two Computable General Equilibrium (CGE) world trade models, and examines the empirical evidence on whether the European Union is more governed by a Classical model of comparative advantage or a Gravity model of new trade theory . It applies time series data between 1970 and 2018 and aims to test the models using Indirect Inference Wald (IIW) on EU trade facts. It extends the Minford and Xu s (2018) UK study, treats EU as a large open economy and simulates worl d variables by using a reduced form VAR of the unknown true world model , applying a Part of Model test. Empirically , the Classical model pass es the tests comfortably whilst the Gravity modelpass es when test power and gravity effect is low, but cannot surv ive in the full test power . Based on a Monte Carlo experiment , we find that the auxiliary model creates substantial but not excessive power for the test . The policy implication of our work is that both models suggest that European Union policymakers should liberaliz e trade policy, because under both model tariff simulations indicate that protection results in general welfare loss.